Masanobu Obara

Last Updated :2019/11/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, ., Professor
E-mail
msobarahiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 1979/04/01, 1985/12/31, University of Occupational and Environmental Health\,Japan, Research Associate
  • 1985/10/01, 1988/05/31, National Cancer Institute, Postdoctoral Fellow
  • 1988/06/01, 1991/03/31, The University of Tokyo, Research Associate
  • 1991/04/01, 1993/03/31, Science and Technology Agency, Special Postdoctoral Researcher
  • 1994/07/01, 2000/03/31, Hiroshima University, Faculty of Science, Assistant Professor
  • 2000/04/01, 2011/06/30, Hiroshima University, Graduate School, Assistant Professor

Educational Backgrounds

  • Kyushu University, Graduate School, Division of Natural Science, Japan, 1977/04, 1979/03

Academic Degrees

  • Doctor of Science, Kyushu University
  • Master of Science, Kyushu University

Educational Activity

  • 【Bachelor Degree Program】School of Science : Biological Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Basic Biology
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Basic Biology

Research Fields

  • Medicine,dentistry, and pharmacy;Clinical internal medicine;Embryonic / Neonatal medicine

Research Keywords

  • morphogenesis
  • extracellular matrix
  • Matrix molecules
  • tumor metastasis
  • Development
  • Globin superfamily

Affiliated Academic Societies

  • The Japanese Cell Biology Society, 1998, 2001
  • The Japanese Connective Tissue Society, 2000, 2001
  • The Japanese Biochemical Society
  • The International Society for Matrix Biology
  • The Zoological Society of Japan
  • Japanese Society of Developmental Biologists

Educational Activity

Course in Charge

  1. 2019, Liberal Arts Education Program1, 1Term, Fundamentals of Biology
  2. 2019, Liberal Arts Education Program1, 3Term, Introduction to Biology
  3. 2019, Undergraduate Education, 1Term, Seminar on Biological Science
  4. 2019, Undergraduate Education, 2Term, Advanced Biology
  5. 2019, Undergraduate Education, 2Term, Animal Physiology B
  6. 2019, Undergraduate Education, Second Semester, Seminar for Molecular Physiology
  7. 2019, Undergraduate Education, First Semester, Special Study for Graduation
  8. 2019, Undergraduate Education, Second Semester, Special Study for Graduation
  9. 2019, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  10. 2019, Graduate Education (Doctoral Program) , First Semester, Seminar for Molecular Physiology
  11. 2019, Graduate Education (Doctoral Program) , Second Semester, Seminar for Molecular Physiology
  12. 2019, Graduate Education (Doctoral Program) , 3Term, Carrier Development for Life Science

Research Activities

Academic Papers

  1. Identification of vitamin A-free cells in a stellate cell-enriched fraction of normal rat liver as myofibroblasts, HISTOCHEMISTRY AND CELL BIOLOGY, 127(2), 161-174, FEB 2007
  2. Characterization of histone lysine-specific demethylase in relation to thyroid hormone-regulated anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49(4), 325-334, MAY 2007
  3. Gene expression and tissue distribution of cytoglobin and myoglobin in the Amphibia and Reptilia: Possible compensation of myoglobin with cytoglobin in skeletal muscle cells of anurans that lack the myoglobin gene, GENE, 398(1-2), 94-102, AUG 15 2007
  4. The third type III module of human fibronectin mediates cell adhesion and migration, JOURNAL OF BIOCHEMISTRY, 147(3), 327-335, MAR 2010
  5. Production of a Non-Triple Helical Collagen alpha Chain in Transgenic Silkworms and Its Evaluation as a Gelatin Substitute for Cell Culture, BIOTECHNOLOGY AND BIOENGINEERING, 106(6), 860-870, AUG 15 2010
  6. Expression of Pleiotrophin in Hepatic Nonparenchymal Cells and Preneoplastic Nodules in Carbon Tetrachloride-induced Fibrotic Rat Liver, Growth Factors, 20(1), 53-60, 20020401
  7. A novel domain of fibronectin revealed by epitope mapping of a monoclonal antibody which inhibits fibroblasts-mediated collagen gel contraction, FEBS Letters, 412(1), 48-52, 19970401
  8. Cloning and characterization of the full length cdna encoding alpha2 type i collagen of bullfrog rana catesbeiana, Gene, 194(2), 283-289, 19970401
  9. Vector-swapping strategy with an exonuclease in cloning and high expression of small pcr products, Biotechniques, 24(1), 44-46, 19980401
  10. Cell-type specific and thyroid hormone-dependent expression of genes of alpha1(i) and alpha2(i) collagen in intestine during amphibian metamorphosis, Matrix Biology, 18(1), 89-103, 19990401
  11. Expression of genes of type i and type ii collagen in the formation and development of the blastema of regenerating newt limb, Developmental Dynamics, 216(1), 59-71, 19990401
  12. The interaction of cellular fibronectin with collagen during fibroblast-mediated contraction of collagen gels, Journal of Investigative Dermatology Symposium Proceedings, 4(2), 190-195, 19990401
  13. Possible involvement of the interaction of the alpha 5 subunit of alpha 5 beta 1 integrin with the synergistic region of the central cell-binding domain of fibronectin in cells to fibronectin binding, Experimental Cell Research, 216(1), 273-276, 19950401
  14. Expression of genes of type I and type II collagen in the formation and development of the blastema of regenerating newt limb, Developmental Dynamics, 216, 59-71, 19990401
  15. The interaction of cellular fibronectin with collagen during fibroblast-mediated contraction of collagen gels, Journal of Investigative Dermatology Symposium Proceedings, 4, 190-195, 19990401
  16. Expression of genes of type I and type II collagen in the formation and development of the blastema of regenerating newt limb, Matrix Biology, 18, 89-103, 19990401
  17. Identification of the ligand binding site for the integrin a9b1 in the third fibronectin type III repeat of tenascin-C, Journal of Biological Chemistry, 273, 11423-11428, 19980401
  18. Site-directed mutagenesis of the cell-binding domain of human fibronecti, Cell, 53, 649-657, 19880401
  19. Attachment, spreading, and locomotion of avian neural crest cells are mediated by multiple adhesion site on fibronectin molecules, EMBO Journal, 4, 2661-2671, 19880401
  20. Analysis of N-myc amplification in relation to disease stage and histologic type in neuroblastoma, Cancer, 60, 820-826, 19870401
  21. The complete primary structures of the typeXII collagen show a chimeric molecule with reiterated fibronectin type III motif, von Willbrand factor A motifs, a domain homologous to a noncollagenous region of type IX collagen, and short collagenous domain with an Arg-Gly-Asp site, Journal of Cell Biology, 115, 209-221, 19910401
  22. Biosynthesis of a novel transformation-sensitive heat-shock protein that binds to collagen. Regulation by mRNA levels and in vitro synthesis of a functional precursor, Journal of Biological Chemistry, 263, 8344-8349, 19880401
  23. Expression of the cell-binding domain of human fibronectin in E. coli. Identification of sequences promoting full to minimal adhesive function, FEBS Lett, 213, 261-264, 19870401
  24. Purification and characterization of nucleolar ribonucleic acid methylase from Ehrlich ascites tumor cells of mice, Biochemistry, 21, 1374-1380, 19820401
  25. Expression of pleiotrophin in hepatic nonparenchymal cells and preneoplastic nodules in carbon tetrachloride-induced fibrotic rat liver. Growth Factors, Growth Factors, 20(1), 53-60, 20020401
  26. a-chymotrypsin catalyzed hydrolysis of peptide substrates, Journal of Biochemistry, 86, 461-468, 19790401
  27. Isolation of nucleolar methylase producing only 5-methylcytidine in ribosomal RNA, Biochemical and Biophysical Research Communications, 104, 241-246, 19820401
  28. Recognition of the ribosomal RNA sutructures by purified nucleolar methylase, Journal of Occupational and Environmental Health, 5, 337-342, 19830401
  29. Isolation of immunologically distinct form of cytochrome P-450 from microsomes of tulip bulbs, Biochemical and Biophysical Research Communications, 115, 46-52, 19830401
  30. Purification of a single major form of microsomal cytochrome P-450 from tulip bulbs, Biosciences Biotechnology and Biochemistry, 49, 2399-2405, 19850401
  31. Heme metabolism and turnover of cytochrome P-450 in tumor-bearing mouse livers., Journal of Occupational and Environmental Health, 7, 269-277, 19850401
  32. Heme metabolism and turnover of cytochrome P-450 in tumor-bearing mouse livers., Biochimica et Biophysica Acta, 910, 171-181, 19870401
  33. Hydrocortisone-induced enhancement of expression and changes in methylation of pepsinogen gene in stomach mucosa of the developing rat, Biochemical and Biophysical Research Communications, 172, 1086-1093, 19900401
  34. Characterization of gene of anuran cathepsin D as metamorphosis-regulating enzyme, Development= Growth and Differentiation, 37, 463-478, 19950401
  35. Human carcinoma cells synthesize and secrete tenascin in vitro, Japanese Journal of Cancer Research, 1073-1080, 19950401
  36. Kdap, a novel gene associated with the stratification of the epithelium, GENE, 256(1-2), 19-27, OCT 3 2000
  37. Developmentally and regionally regulated participation of epidermal cells in the formation of collagen lamella of anuran tadpole skin, DEVELOPMENT GROWTH & DIFFERENTIATION, 42(6), 571-580, DEC 2000
  38. Membrane-type 1 matrix metalloproteinase is induced in decidual stroma without direct invasion by trophoblasts, MOLECULAR HUMAN REPRODUCTION, 7(3), 271-277, MAR 2001
  39. Expression of pleiotrophin in hepatic nonparenchymal cells and preneoplastic nodules in carbon tetrachloride-induced fibrotic rat liver, GROWTH FACTORS, 20(1), 53-60, MAR 2002
  40. Lineage of anuran epidermal basal cells and their differentiation potential in relation to metamorphic skin remodeling, DEVELOPMENT GROWTH & DIFFERENTIATION, 44(3), 225-238, JUN 2002
  41. Characterization of human stellate cell activation-associated protein and its expression in human liver, BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1577(3), 471-475, SEP 27 2002
  42. Metamorphosis-dependent transcriptional regulation of xak-c, a novel Xenopus type I keratin gene, DEVELOPMENTAL DYNAMICS, 225(4), 561-570, DEC 2002
  43. Unique expression patterns of matrix metalloproteinases in regenerating newt limbs, DEVELOPMENTAL DYNAMICS, 226(2), 366-376, FEB 2003
  44. Metal ion-responsive transgenic Xenopus laevis as an environmental monitoring animal, ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 13(3), 153-159, APR 2003
  45. Molecular identification of the skin transformation center of anuran larval skin using genes of Rana adult keratin (RAK) and SPARC as probes, DEVELOPMENT GROWTH & DIFFERENTIATION, 45(5-6), 515-526, OCT-DEC 2003
  46. Typing of hepatic nonparenchymal cells using fibulin-2 and cytoglobin/STAP as liver fibrogenesis-related markers, HISTOCHEMISTRY AND CELL BIOLOGY, 122(1), 41-49, JUL 2004
  47. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, NOV 2014
  48. EXPRESSION OF EGFP GENES CARRYING MOUSE METALLOTHIONEIN GENE IN TRANSGENIC XENOPUS AS AN INDICATOR OF ENVIRONMENTAL POLLUTION(Behavior Biology and Ecology)Proceedings of the Seventy-First Annual Meeting of the Zoological Society of Japan, Zoological science, 17(0), 20001215
  49. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs., In vitro cellular & developmental biology. Animal, 2015
  50. Metal ion-responsive transgenic Xenopus laevis as an environmental monitoring animal., Environmental toxicology and pharmacology, 13(3), 2003

Publications such as books

  1. 2001/07, Gene Therapy of liver fibrosis by inhibiting TGF-beta signaling. , International Conference on New Strategies for the Treatment of liver Fibrosis= Abstract Book, 2001, 07
  2. 2001/05, Gene therapy of liver fibrosis by inhibiting TGF-beta signaling. , Connective Tissue, 2001
  3. 1999/03, Unique expression of genes of type I and type II collagens in apical epidermal cap, blastema, muscle and cartilage of regenerating newt limb. , The Frontiers of the Biology of Amphibia= Abstract Book, 1999, 03
  4. 1998/07, Spatiotemporal expression of a1(I) and a1(II) collagen genes during forelimb regeneration , 4th CGGH symposium-Frontiers in Matrix Biology= Abstract Book, 1998, 07
  5. 1998/07, A possible involvement of epithelial cells in the formation of thick lamella of collagen type I in the anuran larval mesenchyme , 4th CGGH symposium-Frontiers in Matrix Biology= Abstract Book, 1998, 07
  6. 1998/07, A possible involvement of epithelial cells in the formation of thick lamella of collagen type I in the anuran larval mesenchyme , 4th CGGH symposium-Frontiers in Matrix Biology= Abstract Book, 1998, 07
  7. 1996/12, The Gly-Arg-Glu-Ser-Lys-Pro sequence of the fibronectin type III4 repeat is required for the fibroblast-mediated collagen gel contraction , Molecular Biology of the Cell, 1996, 12
  8. 1988/11, In vivo short-term assay for identification of organ-specific carcinogen and promoter , UOEH, 1988
  9. 1988/09, A novel transformation-sensitive heat shock protein (HSP 47) , Hyperthermic Oncology, 1988
  10. 1985/06, Structure of the chicken fibronection gene , Elsevier, 1985
  11. 1981/09, Role of fibronectin in adhesion, migration and metastasis , Cancer Investigation, 1989

Social Activities

Organizing Academic Conferences, etc.

  1. 1998/06
  2. 1998/06
  3. 1995/02
  4. 1995/02