shinichi tate

Last Updated :2024/04/03

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
tatehiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 1999/04/01, 2002/03/31, Japan Advanced Institute of Science and Technology, New Material Center, Associate Professor
  • 2006/04/01, 2021/03/31, Hiroshima University, Graduate School of Science, Professor
  • 1989/04/01, 1992/12/31, Tokyo Metropolitan Institute of Medical Science, Laboratory for Biologically Active Molecules, Researcher
  • 1993/01/01, 1999/03/31, Tokyo Metropolitan University, Department of Chemistry, Faculty of Science, Assistant Professor
  • 2002/04/01, 2005/09/30, Biomolecular Engineering Research Institute, Department of Functional Regulation, Director
  • 2005/10/01, 2008/03/31, Japan Science and Technology Agency, Researcher
  • 1997/07/01, 1997/11/31, NIH, NIDDK, Chemical Physics, Visiting Scientist
  • 1996/06/01, 1996/09/30, ETH, Institute of Molecular Biology & Biophysics, Visiting Scientist

Educational Backgrounds

  • The University of Tokyo, Graduate School, Division of Pharmaceutical Sciences, Japan, 1987/04, 1989/03
  • The University of Tokyo, Graduate School, Division of Pharmaceutical Sciences, Japan, 1985/04, 1987/03
  • The University of Tokyo, Faculty of Pharmaceutical Science, Japan, 1981/04, 1985/03

Academic Degrees

  • Doctor of Philosophy in Pharmaceutical Science, The University of Tokyo
  • Master of Pharmaceutical Science, The University of Tokyo

Research Fields

  • Biology;Biological Science;Structural biochemistry

Research Keywords

  • Protein structure
  • Protein structural dynamics
  • Anisotropic spin interaction
  • Nuclear spin relaxation
  • NMR

Educational Activity

Course in Charge

  1. 2024, Liberal Arts Education Program1, 2Term, Modern Chemistry
  2. 2024, Undergraduate Education, First Semester, Special Study for Graduation
  3. 2024, Undergraduate Education, Second Semester, Special Study for Graduation
  4. 2024, Undergraduate Education, 4Term, Biopolymer Chemistry
  5. 2024, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
  6. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  7. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  8. 2024, Graduate Education (Master's Program) , 1Term, Molecular Biophysics
  9. 2024, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
  10. 2024, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
  11. 2024, Graduate Education (Master's Program) , First Semester, Exercises in Life Science A
  12. 2024, Graduate Education (Master's Program) , Second Semester, Exercises in Life Science B
  13. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  14. 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  15. 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
  16. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  17. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
  18. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E

Research Activities

Academic Papers

  1. Sequential backbone resonance assignments of the E.coli dihydrofolate reductase Gly67Val mutant: folate complex, Biomolecular NMR Assignments, 1-5, 20151019
  2. Allosteric Breakage of the Hydrogen Bond within the Dual-Histidine Motif in the Active Site of Human Pin1 PPlase, BIOCHEMISTRY, 54(33), 5242-5253, 20150825
  3. Coating the outer surface of glass nanopipette with chlorobenzene-terminated polysiloxane, e-Journal of Surface Science and Nanotechnology, 13, 79-84, 20150307
  4. The C113D Mutation in Human Pin1 Causes Allosteric Structural Changes in the Phosphate Binding Pocket of the PPIase Domain through the Tug of War in the Dual-Histidine Motif, BIOCHEMISTRY, 53(34), 5568-5578, 20140902
  5. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834(12), 2782-2794, 20131213
  6. Redox-sensitive structural change in the A-domain of HMGB1 and its implication for the binding to cisplatin modified DNA, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 441(4), 701-706, 20131129
  7. Preferential domain orientation of HMGB2 determined by the weak intramolecular interactions mediated by the interdomain linker, CHEMICAL PHYSICS, 419, 212-223, 20130620
  8. Phosphorylation-Coupled Intramolecular Dynamics of Unstructured Regions in Chromatin Remodeler FACT, BIOPHYSICAL JOURNAL, 104(10), 2222-2234, 20130521
  9. Nucleosome structural changes induced by binding of non-histone chromosomal proteins HMGN1 and HMGN2, FEBS OPEN BIO, 3, 184-191, 20130320
  10. Structural implication for the impaired binding of W150A mutant LOX-1 to oxidized low density lipoprotein, OxLDL, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824(5), 739-749, 20120512
  11. Secondary-structure analysis of alcohol-denatured proteins by vacuum-ultraviolet circular dichroism spectroscopy, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 80(1), 281-293, 20120112
  12. Complementary use of NMR to X-ray crystallography for the analysis of protein morphological change in solution, Current Trends in X-ray Crystallography (Ed. Chandrasekaran, A.), 409-436, 20111201
  13. The NMR Structure of FliK, the Trigger for the Switch of Substrate Specificity in the Flagellar Type III Secretion Apparatus, JOURNAL OF MOLECULAR BIOLOGY, 409(4), 558-573, 20110617
  14. Surface plasmon resonance study on functional significance of clustered organization of lectin-like oxidized LDL receptor (LOX-1), BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1814(2), 345-354, 20110201
  15. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804(4), 846-855, 20100410
  16. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147(4), 591-599, 20100410
  17. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824(3), 511-519, 20100301
  18. Proline cis/trans-Isomerase Pin1 Regulates Peroxisome Proliferator-activated Receptor gamma Activity through the Direct Binding to the Activation Function-1 Domain, JOURNAL OF BIOLOGICAL CHEMISTRY, 285(5), 3126-3132, 20100129
  19. Comprehensive secondary-structure analysis of disulfide variants of lysozyme by synchrotron-radiation vacuum-ultraviolet circular dichroism, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 77(1), 191-201, 20091009
  20. Phosphorylated Intrinsically Disordered Region of FACT Masks Its Nucleosomal DNA Binding Elements, JOURNAL OF BIOLOGICAL CHEMISTRY, 284(36), 24610-24621, 20090904
  21. Anisotropic nuclear spin interactions for the morphology analysis of proteins in solution by NMR spectroscopy, Anal.Sci., 24, 39-50, 20080110
  22. Anisotropic nuclear spin interactions for the morphology analysis of proteins in solution by NMR spectroscopy, ANALYTICAL SCIENCES, 24(1), 39-50, 20080101
  23. Chimeric structural stabilities in the coiled-coil structure of the NECK domain in human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1), JOURNAL OF BIOCHEMISTRY, 141(6), 855-866, 20070607
  24. Oxidized low-density lipoprotein receptor, LOX-1, on the endothelial cell - the receptor structure and functions of LOX-1 in atherogenesis, J.Biol.Macromol., 7(2), 11-22, 20070601
  25. Tautomerism of histidine 64 associated with proton transfer in catalysis of carbonic anhydrase, JOURNAL OF BIOCHEMISTRY, 282(13), 9649-9656, 20070330
  26. Structure and mode of ligand recognition of the oxidized LDL receptor, LOX-1, Functional and structural biology on the lipo-network, 179-198, 20060601
  27. Distinct DNA methylation activity of Dnmt3a and Dnmt3b towards naked and nucleosomal DNA, JOURNAL OF BIOCHEMISTRY, 139(3), 503-515, 20060401
  28. Assembly and disassembly of nucleosome core particles containing histone variants by human nucleosome assembly protein I, MOLECULAR AND CELLULAR BIOLOGY, 25(23), 10639-10651, 20051205
  29. A genetic algorithm taking account of substructures for NMR three-dimensional protein protein structure determination, Evolutionary Computation, 2, 1761-1768, 20051001
  30. Alteration of the nucleosomal DNA path in the crystal structure of a human nucleosome core particle, NUCLEIC ACIDS RESEARCH, 33(10), 3424-3434, 20050610
  31. Resonance assignments for the DNA binding domain of ERCC-1/XPF heterodimer, JOURNAL OF BIOMOLECULAR NMR, 32(2), 175-175, 20050605
  32. Crystal structure of human lectin-like, oxidized low-density lipoprotein receptor 1 ligand binding domain and its ligand recognition mode to OxLDL, STRUCTURE, 13(6), 905-917, 20050605
  33. Purification, crystallization and preliminary X-ray analysis of the ligand-binding domain of human lectin-like oxidized low-density lipoprotein receptor 1 (LOX-1), ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 61, 524-527, 20050505
  34. Solution structure of the HMG-box domain in the SSRP1 subunit of FACT, JOURNAL OF BIOMOLECULAR NMR, 32(1), 83-88, 20050505
  35. Molecular-orientation analysis based on alignment-induced TROSY chemical shift changes, JOURNAL OF MAGNETIC RESONANCE, 171(2), 284-292, 20041204
  36. Domain Architectures and characterization of an RNA-binding protein, TLS, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(43), 44834-44840, 20041022
  37. Measurement of N-15 chemical shift anisotropy in a protein dissolved in a dilute liquid crystalline medium with the application of magic angle sample spinning, JOURNAL OF MAGNETIC RESONANCE, 163(1), 163-173, 20030703
  38. Reelin molecules assemble together to form a large protein complex, which is inhibited by the function-blocking CR-50 antibody, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 97(17), 9729-9734, 20000815
  39. The NMR structure of a DNA dodecamer in an aqueous dilute liquid crystalline phase, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 122(26), 6190-6200, 20000705
  40. Structural comparison between wild-type and P25S human cystatin A by NMR spectroscopy, JOURNAL OF STRUCTURAL AND FUNCTIONAL GENOMICS, 1(1), 26-42, 20000201
  41. Analysis of the relationship between enzyme activity and its internal motion using nuclear magnetic resonance: 15N relaxation studeis of wild-type and mutant lysozyme, JOURNAL OF MOLECULAR BIOLOGY, 286(5), 1547-1565, 19990401
  42. Mutational analysis of the reactive site loop of Streptomyces metalloproteinase inhibitor, SMPI, JOURNAL OF BIOCHEMISTRY, 125(1), 202-209, 19990101
  43. Solution structure of human acidic fibroblast growth factor and interaction with heparin-derived hexasaccharide, JOURNAL OF BIOMOLECULAR NMR, 13(1), 11-24, 19990101
  44. 31p NMR relaxation studies of the local conformational dynamics in a GNRA tetraloop, Nucleic acids symposium series, 39, 125-126, 19981201
  45. NMR structure of the Streptomyces metalloproteinase inhibitor, SMPI, isolated from Streptomyces nigrescens TK-23: Another example of an ancestral beta gamma-crystallin precursor structure, JOURNAL OF MOLECULAR BIOLOGY, 282(2), 421-433, 19980918
  46. Elucidation of the mode of interaction of thermolysin with a proteinaceous metalloproteinase inhibitor, SMPI, based on a model complex structure and a structural dynamics analysis, JOURNAL OF MOLECULAR BIOLOGY, 282(2), 435-446, 19980918
  47. Collision-induced dissociation spectra obtained by Fourier transform ion cyclotron resonance mass spectrometry using a C-13, N-15-doubly depleted protein, ANALYTICAL CHEMISTRY, 70(15), 3333-3336, 19980801
  48. NMR with C-13, N-15-doubly-labeled DNA: The Antennapedia homeodomain complex with a 14-mer DNA duplex, JOURNAL OF BIOMOLECULAR NMR, 12(1), 25-37, 19980701
  49. C5' methylene proton signal assignment of DNA/RNA oligomers labeled with C5'-monodeuterated nucleosides by H-1-P-31 HSQC spectroscopy, MAGNETIC RESONANCE IN CHEMISTRY, 34, S40-S46, 19971201
  50. Measurement of (3)J(C2'P) scalar couplings in a 17 kDa protein complex with C-13,N-15-Labeled DNA distinguishes the B-I and B-II phosphate conformations of the DNA, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 119(41), 9901-9902, 19971015
  51. Nmr spectroscopy of nucleic acids, FASEB JOURNAL, 11(9), A1122-A1122, 19970731
  52. Selective multiple labeling strategy to obtain accurate NMR parameters for nucleic acids. Conformational analysis around the glycosidic bond., Nucleic acids symposium series, 37, 75-76, 19961201
  53. Synthesis of stereoselectively 5'-monodeuterated nucleoside with defined S/R-ratios. An application to the assignment of 5'-methylene signals of DNA oligomers., Nucleic acids symposium series, 37, 35-36, 19961201
  54. Structural analysis of DNA by isotope-aided NMR: Application of 13C/2H-doubly labeled DNA oligomers, Nucleic acids symposium series, 35, 17-18, 19961201
  55. Synthesis of stereoselectively 13C/2H-doubly labeled DNA oligomers by the combined use of chemical and enzymatic reactions., Nucleic acids symposium series, 37, 73-73, 19961201
  56. NMR spectral and structural studies of an RNA hairpin by specific isotope labeling method, Nucleic acids symposium series, 35, 93-94, 19961201
  57. SOLUTION STRUCTURE OF A HUMAN CYSTATIN-A VARIANT, CYSTATIN-A(2-98) M65L, BY NMR-SPECTROSCOPY - A POSSIBLE ROLE OF THE INTERACTIONS BETWEEN THE N-TERMINI AND C-TERMINI TO MAINTAIN THE INHIBITORY ACTIVE FORM OF CYSTATIN-A, BIOCHEMISTRY, 34(45), 14637-14648, 19951114
  58. HUMAN CYSTATIN-A IS INACTIVATED BY ENGINEERED TRUNCATION - THE NH2-TERMINAL REGION OF THE CYSTEINE PROTEINASE-INHIBITOR IS ESSENTIAL FOR EXPRESSION OF ITS INHIBITORY ACTIVITY, BIOCHEMISTRY, 34(38), 12185-12192, 19950926
  59. SIGNIFICANCE OF THE HIGHLY CONSERVED GLY-4 RESIDUE IN HUMAN CYSTATIN-A, JOURNAL OF BIOCHEMISTRY, 118(3), 635-642, 19950901
  60. STEREOSPECIFIC MEASUREMENTS OF THE VICINAL H-1-P-31 COUPLING-CONSTANTS FOR THE DIASTEREOTOPIC C5' METHYLENE PROTONS IN A DNA DODECAMER WITH A C-13/H-2 DOUBLY LABELED RESIDUE - CONFORMATIONAL-ANALYSIS OF THE TORSION ANGLE-BETA, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 117(27), 7277-7278, 19950712
  61. TISSUE ACCUMULATION OF SULFATIDE AND G(M3) GANGLIOSIDE IN A PATIENT WITH VARIANT FARBER-DISEASE, CLINICA CHIMICA ACTA, 234, 23-36, 19950131
  62. SEQUENTIAL BACKBONE ASSIGNMENT IN C-13-LABELED DNA BY THE H-1, C-13, P-31 TRIPLE-RESONANCE EXPERIMENT, HCP-CCH-COSY, JOURNAL OF MAGNETIC RESONANCE SERIES B, 106(1), 89-91, 19950131
  63. New strategy for stable-isotope aided multi-dimensional NMR spectroscopy of DNA oligomer, Stable isotope applications in biomolecular structure and mechanisms, 127-144, 19941201
  64. Preparation of isotopically labeled DNA oligomers and their application to heteronuclear NMR spectroscopy, Nucleic acids symposium series, 31, 295-296, 19941201
  65. TERTIARY STRUCTURE OF ERABUTOXIN-B IN AQUEOUS-SOLUTION AS ELUCIDATED BY 2-DIMENSIONAL NUCLEAR-MAGNETIC-RESONANCE, JOURNAL OF MOLECULAR BIOLOGY, 240(2), 155-166, 19940708
  66. PREPARATION AND HETERONUCLEAR 2D NMR-SPECTROSCOPY OF A DNA DODECAMER CONTAINING A THYMIDINE RESIDUE WITH A UNIFORMLY C-13-LABELED DEOXYRIBOSE RING, JOURNAL OF BIOMOLECULAR NMR, 4(4), 581-586, 19940708
  67. AN ALTERNATIVE TRIPLE-RESONANCE METHOD FOR THE THROUGH-BOND CORRELATION OF INTRANUCLEOTIDE H1' AND H8 NMR SIGNALS OF PURINE NUCLEOTIDES - APPLICATION TO A DNA DODECAMER WITH FULLY C-13/N-15-LABELED DEOXYADENOSINE RESIDUES, JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 116(13), 5977-5978, 19940629
  68. CRYSTALLIZATION AND X-RAY STUDIES OF THE DNA-BINDING DOMAIN OF OMPR PROTEIN, A POSITIVE REGULATOR INVOLVED IN ACTIVATION OF OSMOREGULATORY GENES IN ESCHERICHIA-COLI, JOURNAL OF MOLECULAR BIOLOGY, 235(2), 780-782, 19940114
  69. NOVEL GANGLIOSIDES CONTAINING THE SIALYL-LE(A) STRUCTURE FROM A HUMAN RECTAL ADENOCARCINOMA, JOURNAL OF BIOLOGICAL CHEMISTRY, 268(35), 26541-26545, 19931215
  70. NOVEL OLIGOSACCHARIDES WITH THE SIALYL-LE(A) STRUCTURE IN HUMAN-MILK, JOURNAL OF BIOCHEMISTRY, 114(4), 504-508, 19931001
  71. A NOVEL DIFUCOSYLATED NEUTRAL GLYCOSPHINGOLIPID FROM THE EGGS OF THE SEA-URCHIN, HEMICENTROTUS-PULCHERRIMUS .2. STRUCTURAL DETERMINATION BY 2-DIMENSIONAL NMR, JOURNAL OF BIOCHEMISTRY, 112(2), 286-289, 19930801
  72. A COMPARATIVE-STUDY OF THE SOLUTION STRUCTURES OF TACHYPLESIN-I AND A NOVEL ANTI-HIV SYNTHETIC PEPTIDE, T22 ([TYR(5,12), LYS(7)]-POLYPHEMUSIN-II), DETERMINED BY NUCLEAR-MAGNETIC-RESONANCE, BIOCHIMICA ET BIOPHYSICA ACTA, 1163(2), 209-216, 19930513
  73. NOVEL STRUCTURE OF THE N-ACETYLGALACTOSAMINE CONTAINING N-GLYCOSIDIC CARBOHYDRATE CHAIN OF BATROXOBIN, A THROMBIN-LIKE SNAKE-VENOM ENZYME, JOURNAL OF BIOCHEMISTRY, 112(1), 68-74, 19920701
  74. STABLE ISOTOPE AIDED NUCLEAR-MAGNETIC-RESONANCE STUDY TO INVESTIGATE THE RECEPTOR-BINDING SITE OF HUMAN INTERLEUKIN-1-BETA, BIOCHEMISTRY, 31(8), 2435-2442, 19920503
  75. A trisialoganglioside containing a sialyl alpha 2-6 N-acetylgalactosamine residue is a cholinergic-specific antigen, Chol-1 alpha, JOURNAL OF BIOCHEMISTRY, 111(3), 287-290, 19920401
  76. REDUCTION OF THE WATER HUMP USING A COMPOSITE REFOCUSING PULSE, JOURNAL OF MAGNETIC RESONANCE, 96(3), 635-643, 19920215
  77. A NOVEL N-15-LABELING METHOD TO SELECTIVELY OBSERVE (NH2)-N-15 RESONANCES OF PROTEINS IN H-1-DETECTED HETERONUCLEAR CORRELATION SPECTROSCOPY, FEBS LETTERS, 297, 39-42, 19920203
  78. Isolation and structural studies of human milk oligosaccharides that are reactive with a monoclonal antibody MSW 113, JOURNAL OF BIOCHEMISTRY, 110(4), 598-604, 19911101
  79. APPLICATION OF THE DEPT SEQUENCE TO THE SEPARATION OF (NH)-N-15 AND (NH2)-N-15 RESONANCES IN H-1-DETECTED N-15 SINGLE-QUANTUM COHERENCE SPECTROSCOPY, JOURNAL OF MAGNETIC RESONANCE, 94(3), 625-630, 19911001
  80. SPIN-LOCK PULSES FOR PURGING AND SHAPING THE WATER RESONANCE IN H-1 DETECTED N-15 SINGLE-QUANTUM-COHERENCE SPECTROSCOPY, JOURNAL OF MAGNETIC RESONANCE, 94(2), 394-400, 19910901
  81. TERTIARY STRUCTURE OF CONOTOXIN GIIIA IN AQUEOUS-SOLUTION, BIOCHEMISTRY, 30(28), 6908-6916, 19910716
  82. NOVEL OLIGOSACCHARIDES WITH THE SIALYL-LEA STRUCTURE IN HUMAN-MILK, BIOCHEMISTRY, 30(11), 2869-2876, 19910319
  83. LOCATION OF PHOSPHORYLATION SITE AND DNA-BINDING SITE OF A POSITIVE REGULATOR, OMPR, INVOLVED IN ACTIVATION OF THE OSMOREGULATORY GENES OF ESCHERICHIA-COLI, FEBS LETTERS, 249(2), 168-172, 19890605
  84. LOCATION OF DNA-BINDING SEGMENT OF A POSITIVE REGULATOR, OMPR, INVOLVED IN ACTIVATION OF THE OMPF AND OMPC GENES OF ESCHERICHIA-COLI, FEBS LETTERS, 242(1), 27-30, 19881219
  85. A Raman spectroscopic study on the sequence dependent conformations of DNA oligomers, Nucleic acids symposium series, 17, 195-198, 19851201

Publications such as books

  1. 2006/08, Functional and Structural Biology on the Lipo-network , 2006, 08, Scholarly Book, Compilation, 8178952327, 198

Awards

  1. 1998, Best Article Award, The Spectroscopical Society of Japan