TETSUSHI SAKUMA

Last Updated :2018/10/01

Affiliations, Positions
Graduate School of Science, Department of Mathematical and Life Sciences, Associate Professor or Lecturer
Web Site
E-mail
tetsushi-sakumahiroshima-u.ac.jp
Self-introduction
I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with my great collaborators.

Basic Information

Major Professional Backgrounds

  • 2012/04/01, 2013/03/31, Japan Society for the Promotion of Science (JSPS), JSPS Research Fellow (PD)
  • 2013/04/01, 2015/03/31, Hiroshima University, Graduate School of Science , Assistant Professor (Special Appointment)
  • 2015/04/01, 2018/03/31, Hiroshima University, Graduate School of Science , Lecturer (Special Appointment)
  • 2018/04/01, Hiroshima University, Graduate School of Science , Lecturer

Educational Backgrounds

  • Osaka University, Japan, 2002/04, 2006/03
  • Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2006/04, 2008/03
  • Hiroshima University, Graduate School of Science, Department of Biological Science, Japan, 2008/04, 2010/03
  • Hiroshima University, Graduate School of Science, Department of Mathematical and Life Sciences, Japan, 2010/04, 2012/03

Academic Degrees

  • Doctor of Science (Ph.D.), Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • School of Science:Biological Sciences, Graduate School of Science:Mathematical and Life Sciences, Graduate School of Science:Mathematical and Life Sciences

Research Fields

  • Biological Sciences;Genome science;System genome science
  • Biological Sciences;Genome science;Medical genome science

Research Keywords

  • Genome editing
  • Epigenome editing
  • TALEN
  • CRISPR-Cas9
  • Gene knockout
  • Gene knockin

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2018, Undergraduate Education, 1Term, Basic Biological Science A
  2. 2018, Undergraduate Education, 3Term, Developmental biology A
  3. 2018, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  4. 2018, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  5. 2018, Graduate Education (Master's Program) , Second Semester, Molecular Genetics
  6. 2018, Graduate Education (Master's Program) , First Semester, Seminar of Molecular Genetics
  7. 2018, Graduate Education (Master's Program) , Second Semester, Seminar of Molecular Genetics
  8. 2018, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences
  9. 2018, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences

Research Activities

Academic Papers

  1. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, MAY 2018
  2. Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, MAR 5 2018
  3. Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, JUL 2018
  4. Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference, PLOS ONE, 13(1), JAN 29 2018
  5. Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, FEB 2018
  6. Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, FEB 16 2018
  7. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, AUG 2018
  8. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, APR 2018
  9. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), NOV 17 2017
  10. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, MAY 19 2017
  11. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, MAY 15 2017
  12. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, MAY 1 2017
  13. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, MAR 2017
  14. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, MAR 15 2017
  15. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, JUL 3 2017
  16. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  17. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  18. TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
  19. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, NOV 28 2016
  20. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, NOV 24 2016
  21. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, NOV 2016
  22. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, MAY 2016
  23. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, MAY 2016
  24. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, MAY 2016
  25. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, MAY 10 2016
  26. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, JUN 2016
  27. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, JUN 2016
  28. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, JUL 7 2016
  29. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, JUL 2016
  30. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), JAN 2016
  31. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, JAN 2016
  32. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11(1), 118-133, JAN 2016
  33. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), DEC 28 2016
  34. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, DEC 2016
  35. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, DEC 20 2016
  36. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, AUG 15 2016
  37. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, APR 2016
  38. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, APR 12 2016
  39. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, OCT 2015
  40. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, OCT 2015
  41. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, OCT 2015
  42. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), NOV 18 2015
  43. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, MAY 22 2015
  44. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, MAR 5 2015
  45. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), MAR 17 2015
  46. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, JUN 9 2015
  47. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, JUL 27 2015
  48. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, JUL 2015
  49. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, JUL 14 2015
  50. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, JUL 1 2015
  51. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, JAN 13 2015
  52. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, FEB 2015
  53. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, FEB 10 2015
  54. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, DEC 2015
  55. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, AUG 6 2015
  56. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, APR 29 2015
  57. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  58. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, SEP 2014
  59. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, OCT 1 2014
  60. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, NOV 2014
  61. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, NOV 20 2014
  62. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, NOV 18 2014
  63. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, MAY 2014
  64. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, MAY 2014
  65. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, JUN 23 2014
  66. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, JUL 2014
  67. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, JUL 16 2014
  68. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, JAN 28 2014
  69. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, JAN 2014
  70. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, JAN 2014
  71. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, JAN 2014
  72. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, JAN 2014
  73. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, JAN 2014
  74. Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 2-13, JAN 2014
  75. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, JAN 2014
  76. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, JAN 15 2014
  77. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, AUG 4 2014
  78. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, AUG 27 2014
  79. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, NOV 5 2013
  80. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, NOV 29 2013
  81. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, MAY 1 2013
  82. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, MAR 1 2013
  83. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, APR 2013
  84. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 2013 Mar
  85. Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 2013 Mar
  86. Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 2013 Feb
  87. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
  88. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 2012 Jun
  89. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 2012 Jul
  90. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
  91. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 2011 Aug
  92. The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 2010 Mar

External Funds

Acceptance Results of Competitive Funds

  1. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  2. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  3. Research Program on Hepatitis , 2017/04/01, 2018/03/31
  4. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  5. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  6. Research Program on Hepatitis , 2016/04/01, 2017/03/31
  7. 2016/04/01, 2017/03/31
  8. KAKENHI, 2016, 2017
  9. KAKENHI, 2015, 2018
  10. KAKENHI, 2013, 2014
  11. KAKENHI, 2012, 2013