TETSUSHI SAKUMA

Last Updated :2018/08/01

Affiliations, Positions
Graduate School of Science, Department of Mathematical and Life Sciences, Associate Professor or Lecturer
Web Site
E-mail
tetsushi-sakumahiroshima-u.ac.jp
Self-introduction
I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with my great collaborators.

Basic Information

Major Professional Backgrounds

  • 2013/04/01, 2015/03/31, Hiroshima University, Graduate School of Science , Assistant Professor (Special Appointment)
  • 2012/04/01, 2013/03/31, Japan Society for the Promotion of Science (JSPS), JSPS Research Fellow (PD)

Educational Backgrounds

  • Hiroshima University, Graduate School of Science, Department of Mathematical and Life Sciences, Japan, 2010/04, 2012/03
  • Hiroshima University, Graduate School of Science, Department of Biological Science, Japan, 2008/04, 2010/03
  • Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2006/04, 2008/03

Academic Degrees

  • Doctor of Science (Ph.D.), Hiroshima University
  • Master of Science, Hiroshima University

Research Fields

  • Biological Sciences;Genome science;System genome science

Research Keywords

  • Genome editing
  • TALEN
  • CRISPR/Cas9
  • Gene knockout
  • Gene knockin

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2018, Undergraduate Education, 1Term, Basic Biological Science A
  2. 2018, Undergraduate Education, 3Term, Developmental biology A
  3. 2018, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  4. 2018, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  5. 2018, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences
  6. 2018, Graduate Education (Master's Program) , Second Semester, Molecular Genetics
  7. 2018, Graduate Education (Master's Program) , First Semester, Seminar of Molecular Genetics
  8. 2018, Graduate Education (Master's Program) , Second Semester, Seminar of Molecular Genetics
  9. 2018, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences

Research Activities

Academic Papers

  1. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  2. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  3. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, JUL 3 2017
  4. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, MAR 15 2017
  5. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, MAR 2017
  6. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, MAY 1 2017
  7. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, MAY 15 2017
  8. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, MAY 19 2017
  9. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, APR 12 2016
  10. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, APR 2016
  11. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, AUG 15 2016
  12. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, DEC 20 2016
  13. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, DEC 2016
  14. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), DEC 28 2016
  15. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), JAN 2016
  16. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, JAN 2016
  17. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11(1), 118-133, JAN 2016
  18. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, JUL 2016
  19. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, JUL 7 2016
  20. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, JUN 2016
  21. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, JUN 2016
  22. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, MAY 10 2016
  23. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, MAY 2016
  24. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, MAY 2016
  25. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, MAY 2016
  26. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, NOV 2016
  27. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, NOV 24 2016
  28. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, NOV 28 2016
  29. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  30. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, APR 29 2015
  31. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, AUG 6 2015
  32. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, DEC 2015
  33. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, FEB 10 2015
  34. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, FEB 2015
  35. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, JAN 13 2015
  36. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, JUL 1 2015
  37. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, JUL 14 2015
  38. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, JUL 2015
  39. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, JUL 27 2015
  40. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, JUN 9 2015
  41. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), MAR 17 2015
  42. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, MAR 5 2015
  43. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, MAY 22 2015
  44. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), NOV 18 2015
  45. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, OCT 2015
  46. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, OCT 2015
  47. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, OCT 2015
  48. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, AUG 27 2014
  49. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, AUG 4 2014
  50. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, JAN 15 2014
  51. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, JAN 2014
  52. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, JAN 2014
  53. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, JAN 2014
  54. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, JAN 2014
  55. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, JAN 2014
  56. Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 2-13, JAN 2014
  57. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, JAN 2014
  58. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, JAN 28 2014
  59. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, JUL 16 2014
  60. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, JUL 2014
  61. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, JUN 23 2014
  62. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, MAY 2014
  63. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, MAY 2014
  64. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, NOV 18 2014
  65. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, NOV 20 2014
  66. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, NOV 2014
  67. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, OCT 1 2014
  68. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, SEP 2014
  69. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
  70. Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 2013 Feb
  71. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 2013 Mar
  72. Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 2013 Mar
  73. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, APR 2013
  74. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, MAR 1 2013
  75. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, MAY 1 2013
  76. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, NOV 29 2013
  77. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, NOV 5 2013
  78. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
  79. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 2012 Jul
  80. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 2012 Jun
  81. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 2011 Aug
  82. The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 2010 Mar

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, 2013, 2014
  2. KAKENHI, 2012, 2013
  3. KAKENHI, 2016, 2017
  4. KAKENHI, 2015, 2018
  5. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  6. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  7. Research Program on Hepatitis , 2016/04/01, 2017/03/31
  8. 2016/04/01, 2017/03/31