TETSUSHI SAKUMA

Last Updated :2018/12/09

Affiliations, Positions
Graduate School of Science, Department of Mathematical and Life Sciences, Associate Professor or Lecturer
Web Site
E-mail
tetsushi-sakumahiroshima-u.ac.jp
Self-introduction
I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with my great collaborators.

Basic Information

Major Professional Backgrounds

  • 2012/04/01, 2013/03/31, Japan Society for the Promotion of Science (JSPS), JSPS Research Fellow (PD)
  • 2013/04/01, 2015/03/31, Hiroshima University, Graduate School of Science , Assistant Professor (Special Appointment)
  • 2015/04/01, 2018/03/31, Hiroshima University, Graduate School of Science , Lecturer (Special Appointment)
  • 2018/04/01, Hiroshima University, Graduate School of Science , Lecturer

Educational Backgrounds

  • Osaka University, Japan, 2002/04, 2006/03
  • Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2006/04, 2008/03
  • Hiroshima University, Graduate School of Science, Department of Biological Science, Japan, 2008/04, 2010/03
  • Hiroshima University, Graduate School of Science, Department of Mathematical and Life Sciences, Japan, 2010/04, 2012/03

Academic Degrees

  • Doctor of Science (Ph.D.), Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • School of Science:Biological Sciences, Graduate School of Science:Mathematical and Life Sciences, Graduate School of Science:Mathematical and Life Sciences

Research Fields

  • Biological Sciences;Genome science;System genome science
  • Biological Sciences;Genome science;Medical genome science

Research Keywords

  • Genome editing
  • Epigenome editing
  • TALEN
  • CRISPR-Cas9
  • Gene knockout
  • Gene knockin

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2018, Liberal Arts Education Program1, 4Term, Introduction to Biology
  2. 2018, Undergraduate Education, 3Term, Developmental biology A
  3. 2018, Undergraduate Education, 1Term, Basic Biological Science A
  4. 2018, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  5. 2018, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  6. 2018, Graduate Education (Master's Program) , Second Semester, Molecular Genetics
  7. 2018, Graduate Education (Master's Program) , First Semester, Seminar of Molecular Genetics
  8. 2018, Graduate Education (Master's Program) , Second Semester, Seminar of Molecular Genetics
  9. 2018, Graduate Education (Doctoral Program) , First Semester, Special Research for Mathematical and Life Sciences
  10. 2018, Graduate Education (Doctoral Program) , Second Semester, Special Research for Mathematical and Life Sciences

Research Activities

Academic Papers

  1. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes, STEM CELL REPORTS, 11(3), 727-740, 20180911
  2. Biased genome editing using the local accumulation of DSB repair molecules system, NATURE COMMUNICATIONS, 9, 20180816
  3. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, 201805
  4. Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, 20180305
  5. Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, 201807
  6. Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference, PLOS ONE, 13(1), 20180129
  7. Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, 201802
  8. Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, 20180216
  9. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, 201808
  10. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, 201804
  11. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), 20171117
  12. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, 20170519
  13. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, 20170515
  14. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, 20170501
  15. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, 201703
  16. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, 20170315
  17. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, 20170703
  18. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  19. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  20. TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
  21. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, 20161128
  22. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, 20161124
  23. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, 201611
  24. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, 201605
  25. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, 201605
  26. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, 201605
  27. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, 20160510
  28. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, 201606
  29. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, 201606
  30. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, 20160707
  31. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, 201607
  32. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), 201601
  33. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, 201601
  34. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11(1), 118-133, 201601
  35. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), 20161228
  36. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, 201612
  37. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, 20161220
  38. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, 20160815
  39. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, 201604
  40. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, 20160412
  41. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, 201510
  42. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, 201510
  43. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, 201510
  44. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), 20151118
  45. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, 20150522
  46. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, 20150305
  47. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), 20150317
  48. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, 20150609
  49. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, 20150727
  50. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, 201507
  51. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, 20150714
  52. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, 20150701
  53. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, 20150113
  54. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, 201502
  55. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
  56. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, 201512
  57. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, 20150806
  58. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, 20150429
  59. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  60. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, 201409
  61. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, 20141001
  62. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, 201411
  63. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 20141120
  64. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, 20141118
  65. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, 201405
  66. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, 201405
  67. Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, 20140623
  68. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, 201407
  69. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, 20140716
  70. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
  71. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, 201401
  72. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, 201401
  73. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, 201401
  74. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, 201401
  75. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, 201401
  76. Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 2-13, 201401
  77. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, 201401
  78. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, 20140115
  79. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, 20140804
  80. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, 20140827
  81. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, 20131105
  82. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, 20131129
  83. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, 20130501
  84. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, 20130301
  85. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
  86. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 201303
  87. Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 201303
  88. Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 201302
  89. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
  90. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 201206
  91. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 201207
  92. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
  93. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 201108
  94. The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 201003

Patented

  1. 5896547, 2016/03/11
  2. 5900942, 2016/03/18
  3. Patent, 5931022, 2016//5//1
  4. Patent, 6246258, 2017//1/1/

External Funds

Acceptance Results of Competitive Funds

  1. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  2. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  3. Research Program on Hepatitis , 2017/04/01, 2018/03/31
  4. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  5. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  6. Research Program on Hepatitis , 2016/04/01, 2017/03/31
  7. Design and functional evaluation of artificial transcriptional activator, 2016/04/01, 2017/03/31
  8. KAKENHI, 2016, 2017
  9. KAKENHI, 2015, 2018
  10. KAKENHI, 2013, 2014
  11. KAKENHI, 2012, 2013