中坪 敬子Keiko Nakatsubo

Last Updated :2017/09/01

所属・職名
大学院理学研究科 生命理学講座 助教
メールアドレス
kmntnhiroshima-u.ac.jp
その他連絡先
広島県東広島市鏡山1-3-1理学部A棟421号室
TEL:082-424-7448 FAX:082-424-7327

基本情報

学位

  • 理学博士 (早稲田大学)
  • 理学修士 (早稲田大学)

教育担当

  • 理学部:生物科学科, 理学研究科:数理分子生命理学専攻, 理学研究科:数理分子生命理学専攻

担当主専攻プログラム

  • 生物学プログラム

研究分野

  • 生物学 / 基礎生物学 / 形態・構造
  • 生物学 / 生物科学 / 発生生物学

研究キーワード

  • 細胞分化
  • 細胞運命決定
  • 細胞外基質
  • 初期発生
  • 形態形成

所属学会

  • 日本動物学会
  • 日本生化学会
  • 日本発生生物学会
  • 日本臨床分子形態学会

教育活動

授業担当

  1. 2017年, 教養教育, セメスター(前期), 教養ゼミ
  2. 2017年, 学部専門, セメスター(後期), 分子遺伝学演習
  3. 2017年, 学部専門, セメスター(後期), 生物科学基礎実験II
  4. 2017年, 学部専門, セメスター(後期), 生物科学基礎実験IV
  5. 2017年, 修士課程・博士課程前期, セメスター(前期), 分子遺伝学セミナー
  6. 2017年, 修士課程・博士課程前期, セメスター(後期), 分子遺伝学セミナー

研究活動

学術論文(★は代表的な論文)

  1. ★, Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers, MEDICAL MOLECULAR MORPHOLOGY, 42巻, 2号, pp.63-pp.69, JUN 2009
  2. ★, Sea urchin arylsulfatase, an extracellular matrix component, is involved in gastrulation during embryogenesis, DEVELOPMENT GENES AND EVOLUTION, 219巻, 6号, pp.281-pp.288, JUN 2009
  3. ★, Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos, Journal of Experimental Zoology, 280巻, 3号, pp.220-pp.230, 19980401
  4. ★, Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNAs during early development, International Journal of Developmental Biology, 42巻, pp.645-pp.651, 19980401
  5. ★, Distributions of H+,K+-ATPase and Cl-,HCO32---ATPase in micromere-derived cells of sea urchin embryos, Differentiation, 35巻, pp.190-pp.196, 19920401
  6. Novel structure of hepatic extracellular matrices containing arylsulfatase A, Okajimas Folia Anatomica Japonica Language: Previous Article http://doi.org/10.2535/ofaj.90.17, 90巻, 90号, pp.17-pp.22, July 25, 2013
  7. Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51巻, 5号, pp.388-pp.396, OCT 2010
  8. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6巻, 5号, pp.572-pp.577, JUN 2006
  9. Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23巻, 1号, pp.9-pp.21, JAN 2006
  10. The micro1 gene is necessary and sufficient for micromere differentiation and mid/hindgut-inducing activity in the sea urchin embryo, DEVELOPMENT GENES AND EVOLUTION, 215巻, 9号, pp.450-pp.459, SEP 2005
  11. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46巻, 4号, pp.335-pp.341, AUG 2004
  12. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46巻, 1号, pp.61-pp.69, FEB 2004
  13. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213巻, 1号, pp.44-pp.49, FEB 2003
  14. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129巻, 22号, pp.5205-pp.5216, NOV 2002
  15. Brachyury homolog (HpTa) is involved in the formation of archenteron and secondary mesenchyme cell differentiation in the sea urchin embryo, ZOOLOGY, 104巻, 2号, pp.99-pp.102, 2001
  16. Sox regulates transcription of the sea urchin arylsulfatase gene, DEVELOPMENT GROWTH & DIFFERENTIATION, 42巻, 4号, pp.429-pp.435, AUG 2000
  17. CAAT sites are required for the activation of the H-pulcherrimus Ars gene by Otx, DEVELOPMENT GENES AND EVOLUTION, 210巻, 12号, pp.583-pp.590, DEC 2000
  18. HpEts implicated in primary mesenchyme cell differentiation of the sea urchin (Hemicentrotus pulcherrimus) embryo, ZYGOTE, 8巻, pp.S33-pp.S34, 2000
  19. Lim1-related homeobox gene (HpLim1) expressed in sea urchin embryo, ZYGOTE, 8巻, pp.S71-pp.S72, 2000
  20. Lim1-related homeobox gene (HpLim1) expressed in sea urchin embryo, Development Growth and Differentiation, 41巻, 3号, pp.273-pp.282, 19990601
  21. Hbox1 and Hbox7 are involved in pattern formation in sea urchin embryos, Development Growth and Differentiation, 41巻, 3号, pp.241-pp.252, 19990601
  22. HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation of the sea urchin embryo, Mechanisms of Development, 80巻, pp.41-pp.52, 19990401
  23. Upstream element of the sea urchin arylsulfatase gene serves as an insulator, Cellular and Molecular Biology, 45巻, 5号, pp.555-pp.565, 19990401
  24. Proximal cis-regulatory elements of sea urchin arylsulfatase gene, Development Growth and Differentiation, 40巻, 5号, pp.537-pp.544, 19981001
  25. Structure and function of a sea urchin orthodenticle-related gene (HpOtx), Developmental Biology, 193巻, 2号, pp.139-pp.145, 19980101
  26. Cloning of cyclin E cDNA of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 14巻, 5号, pp.791-pp.794, 19971001
  27. Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene, Developmental Biology, 181巻, pp.284-pp.295, 19970401
  28. Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore, Development Growth and Differentiation, 39巻, pp.373-pp.379, 19970401
  29. A cis-regulatory element within the 5’ flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 39巻, pp.469-pp.476, 19970401
  30. cDNA cloning of Na+, K+-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 13巻, 6号, pp.833-pp.841, 19960401
  31. Changes in insulin-binding capacity of the plasma membrane fraction during culture in vitro of cells derived from micromeres of 16-cell-stage sea urchin embryos, Development Growth and Differentiation, 36巻, pp.289-pp.298, 19940401
  32. Several cell responses to insulin of cultured cells derived from micromeres, isolated from sea urchin embryos at the 16 cell stage, Development Growth and Differentiation, 36巻, pp.397-pp.408, 19940401
  33. カルシウム沈着のしくみ, 遺伝, 48巻, 4号, pp.40-pp.46, 19940401
  34. Isolation of cDNA for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development, Developmental Biology, 153巻, 2号, pp.250-pp.258, 19921001
  35. Change in the activity of Na+,K+-ATPase in embryos of the sea urchin, Hemicentrotus pulcherrimus, during early development, Development Growth and Differentiation, 34巻, pp.379-pp.385, 19920401
  36. Expression of Na+,K+-ATPase alpha-subunit in animalized and vegetalized embryos of the sea urchin Hemicentrotus pulcherrimus, Development Growth and Differentiation, 34巻, pp.677-pp.684, 19920401
  37. Does protein phosphorylation by protein kinase C support pseudopodial cable growth in cultured micromere-derived cells of the sea urchin, Hemicentrotus pulcherrimus?, Development Growth and Differentiation, 32巻, pp.647-pp.655, 19900401
  38. Probable contribution of protein phosphorylation by protein kinase C to spicule formation in sea urchin embryos, Development Growth and Differentiation, 32巻, pp.335-pp.342, 19900401
  39. Changes in the activities of H+,K+-ATPase and Na+,K+- ATPase in cultured cells derived from micromeres of sea urchin embryos with special reference to their roles in spicule rod formation, Development Growth and Differentiation, 31巻, pp.171-pp.178, 19890401
  40. Probable participation of mitochondrial Ca2+ transport in calcification of spicules and morphogenesis in sea urchin embryos, Zoological Science, 5巻, pp.93-pp.102, 19880401
  41. Development of sea urchin embryos in artificial sea water containing Br- in place of Cl-, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29巻, pp.599-pp.605, 19870401
  42. Inhibitory effect of omeprazole, a specific inhibitor of H+,K+-ATPase, on spicule formation in sea urchin embryos and in cultured micromere-derived cells, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29巻, pp.591-pp.597, 19870401
  43. Probable role of allylisothiocyanate-sensitive H+,K+-ATPase in spicule calcification in embryos of the sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 29巻, pp.57-pp.70, 19870401
  44. Change in the activity of Cl-,HCO32-ATPase in microsome fraction during early development of the sea urchin, Hemicentrotus pulcherrimus, Journal of Biochemistry, 100巻, pp.1607-pp.1615, 1986 Dec
  45. Carbonic anhydrase activity in developing sea urchin embryos with special reference to calcification of spicules, Cell Differentiation, 18巻, pp.257-pp.262, 1986 Jun
  46. Inhibitory effects of ethacrynic acid, furosemide, and nifedipine on the calcification of spicules in cultures of micromeres isolated from sea-urchin eggs, Differentiation, 153巻, 30号, pp.197-pp.204, 1986 March
  47. Inhibition of 45Ca2+ uptake in the eggs and embryos of the sea urchin, Anthocidaris crassispina, by several calcium antagonists, anion transport inhibitor and chloride transport inhibitors, Journal of Experimental Zoology, 235巻, pp.281-pp.288, 19850401
  48. Mechanism for electrosilent Ca2+ transport to cause calcification of spicules in sea urchin embryos., Experimental Cell Research, 159巻, pp.80-pp.90, 1985 Jul
  49. Stage specific effects of Zn2+ on sea urchin embryogenesis, Development Growth and Differentiation, 26巻, pp.317-pp.327, 1984 Oct
  50. Stage specific effects on sea urchin embryogenesis of Zn2+, Li+, several inhibitors of cAMP-phosphodiestrase and inhibitors of protein synthesis., Development Growth and Differentiation, 25巻, pp.249-pp.260, 19830401

著書等出版物

  1. シリーズ(応用動物科学/バイオサイエンス)10 無脊椎動物の発生, 海綿動物からナメクジウオまで多様な無脊椎動物の発生パターンと遺伝子レベルの調節機構を解説した。, 朝倉書店, 2005年, 03, 単行本(学術書), 共著, ISBN9784254176766 , 132
  2. 生物教育用語集 , 東京大学出版会, 1998年, 9, 事典・辞書, 共著, 日本動物学会 日本植物学会編, ISBN978-4-13-062210-3

受賞

  1. 2010年09月25日, 第42回日本臨床分子形態学会論文賞, 第42回日本臨床分子形態学会会長, Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers
  2. 2007年10月, 広島大学女性研究者奨励賞, 広島大学, 細胞外基質アリールスルファターゼの分子環境と細胞形態制御機構の解析

社会活動

委員会等委員歴

  1. 男女共同参画委員会委員, 2015年03月, 2016年09月, 公益社団法人日本動物学会
  2. 男女共同参画委員会委員長, 2013年02月, 2014年09月, 公益社団法人日本動物学会

学術会議等の主催

  1. The international symposium on fertilization and development of sea urchin and marine invertebrates, 地方委員, 1999年12月
  2. 日本発生生物学会第24回大会, 準備委員, 1991年05月