小池 透Tohru Koike

Last Updated :2019/08/12

所属・職名
大学院医系科学研究科(薬) (薬学)医薬分子機能科学 教授
メールアドレス
tkoikehiroshima-u.ac.jp
その他連絡先
広島市南区霞1丁目2番3号 薬学部研究棟3階東館308
TEL:082-257-5323
自己紹介
私たちの研究室は,リン酸基結合タグ分子「フォスタグ」を使ったリン酸化生体物質の分離・精製・検出法を開発しています。

基本情報

学位

  • 薬学博士 (広島大学)
  • 薬学修士 (広島大学)

研究分野

  • 医歯薬学 / 薬学 / 物理系薬学

研究キーワード

  • フォスタグ技術
  • リン酸化プロテオミクス
  • チオタグ技術

教育活動

授業担当

  1. 2019年, 学部専門, 4ターム, 一般化学
  2. 2019年, 学部専門, 1ターム, 薬品物理化学
  3. 2019年, 学部専門, セメスター(後期), 物理化学実習
  4. 2019年, 学部専門, 通年, 基礎研究I
  5. 2019年, 学部専門, 通年, 基礎研究II
  6. 2019年, 学部専門, 通年, 臨床研究I
  7. 2019年, 学部専門, 通年, 臨床研究II
  8. 2019年, 学部専門, 通年, 臨床研究III
  9. 2019年, 修士課程・博士課程前期, セメスター(後期), 医薬分子機能科学特論
  10. 2019年, 修士課程・博士課程前期, セメスター(前期), 医薬分子機能科学特論演習
  11. 2019年, 修士課程・博士課程前期, セメスター(後期), 医薬分子機能科学特論演習
  12. 2019年, 修士課程・博士課程前期, セメスター(前期), 医薬分子機能科学特別実習
  13. 2019年, 修士課程・博士課程前期, セメスター(後期), 医薬分子機能科学特別実習
  14. 2019年, 博士課程・博士課程後期, セメスター(前期), 医薬分子機能科学特別演習
  15. 2019年, 博士課程・博士課程後期, セメスター(後期), 医薬分子機能科学特別演習
  16. 2019年, 博士課程・博士課程後期, セメスター(前期), 医薬分子機能科学特別実験
  17. 2019年, 博士課程・博士課程後期, セメスター(後期), 医薬分子機能科学特別実験
  18. 2019年, 修士課程・博士課程前期, セメスター(前期), 医薬分子機能科学特論演習
  19. 2019年, 修士課程・博士課程前期, セメスター(後期), 医薬分子機能科学特論演習
  20. 2019年, 修士課程・博士課程前期, セメスター(前期), 医薬分子機能科学特別研究
  21. 2019年, 修士課程・博士課程前期, セメスター(後期), 医薬分子機能科学特別研究
  22. 2019年, 博士課程・博士課程後期, セメスター(前期), 医薬分子機能科学特別演習
  23. 2019年, 博士課程・博士課程後期, セメスター(後期), 医薬分子機能科学特別演習
  24. 2019年, 博士課程・博士課程後期, セメスター(前期), 医薬分子機能科学特別研究
  25. 2019年, 博士課程・博士課程後期, セメスター(後期), 医薬分子機能科学特別研究

教育に関する受賞

  • 2011年03月11日, 広島大学大学院 医歯薬学総合研究科教員表彰, 医歯薬学総合研究科

研究活動

学術論文(★は代表的な論文)

  1. Validation of Cis and Trans Modes in Multistep Phosphotransfer Signaling of Bacterial Tripartite Sensor Kinases by Using Phos-tag SDS-PAGE, PLOS ONE, pp. e0148294, 2016
  2. A Phos-tag SDS-PAGE method that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1, Proteomics, 16巻, pp. 1825-1836, 2016
  3. Advances in Phos-tag-based methodologies for separation and detection of the phosphoproteome, BIOCHIMICA ET BIOPHYSICA ACTA: PROTEINS AND PROTEOMICS, 1854巻, 6号, pp. 601-608, 2015
  4. Functional Characterization of the Receiver Domain for Phosphorelay Control in Hybrid Sensor Kinases, PLOS ONE, 10巻, 7号, 2015
  5. Phos-tag SDS-PAGEゲルからの標的タンパク質の転写効率改善, Bunseki Kagaku, 64巻, 7号, pp. 501-509, 2015
  6. The Cutting Edge of Affinity Electrophoresis Technology, Proteomes, 3巻, pp. 42-55, 2015
  7. Expression and phosphorylation state analysis of intracellular protein kinases using Multi-PK antibody and Phos-tag SDS-PAGE, MethodsX, 2巻, pp. 469-474, 2015
  8. Tips on improving the efficiency of electrotransfer of target proteins from Phos-tag SDS-PAGE gel, Proteomics, 14巻, 21-22号, pp. 2437-2442, 2014
  9. Profiling of protein thiophosphorylation by Phos-tag affinity electrophoresis: Evaluation of adenosine 5-O-( 3-thiotriphosphate) as a phosphoryl donor in protein kinase reactions, Proteomics, 14巻, 6号, pp. 668-679, 2014
  10. Simple enrichment of thiol-containing biomolecules by using zinc(II)-cyclen-functionalized magnetic beads, Journal of Separation Science, 37巻, 13号, pp. 1601-1609, 2014
  11. A Phos-tag-based fluorescence quenching system for activity assay and inhibitor screening for alkaline phosphatase, American Journal of Analytical Chemistry, 5巻, pp. 796-804, 2014
  12. Identification of two phosphorylated species of beta-catenin involved in the ubiquitin-proteasome pathway by using two-dimensional Phos-tag affinity electrophoresis, Journal of Electrophoresis, 58巻, pp. 1-4, 2014
  13. アフィニティー電気泳動技術の最前線, ぶんせき, 7巻, pp. 365-371, 2014
  14. A Phos-tag-based magnetic-bead method for rapid and selective separation of phosphorylated biomolecules, Journal of Chromatography B, 925巻, pp. 86-94, 2013
  15. Increase in Lipophilicity of Enalaprilat by Complexation with Copper (II) or Zinc (II) Ions, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 133巻, 10号, pp. 1135-1141, 2013
  16. Sandwich assay for phosphorylation of protein multiplexes by using antibodies and Phos-tag, Analytical Biochemistry, 438巻, 2号, pp. 104-106, 2013
  17. タンパク質リン酸化修飾の高感度検出法と新しいオミクス技術としての展開, 生化学, 85巻, 6号, pp. 447-455, 2013
  18. Phos-tag Toyopearlを用いた生理活性リン酸モノエステル型脂質の分析法の開発, 脂質生化学研究, 55巻, pp. 32-35, 2013
  19. Phos-tag-Based Microarray Techniques Advance Phosphoproteomics, Journal of Proteomics & Bioinformatics, S6巻, pp. 1-8, 2013
  20. A Laborsaving, Timesaving, and More Reliable Strategy for Separation of Low-Molecular-Mass Phosphoproteins in Phos-tag Affinity Electrophoresis, Intrenational Journal of Chemistry, 4巻, 5号, pp. e1-e8, 2012
  21. Highly sensitive detection of protein phosphorylation by using improved Phos-tag Biotin, Proteomics, 12巻, 7号, pp. 932-937, 2012
  22. Separation and identification of four distinct serine-phosphorylation states of ovalbumin by Phos-tag affinity electrophoresis, Electrophoresis, 33巻, 5号, pp. 849-855, 2012
  23. Phos-tag SDS-PAGE systems for phosphorylation profiling of proteins with a wide range of molecular masses under neutral pH conditions, Proteomics, 12巻, 2号, pp. 192-202, 2012
  24. リン酸化プロテオミクスのためのPhos-tagテクノロジー, Bunseki Kagaku, 61巻, pp. 469-487, 2012
  25. フォスタグケミストリー, 生物物理化学, 56巻, pp. 3-7, 2012
  26. 中性条件で泳動を行うPhos-tag SDS-PAGEを用いたリン酸化タンパク質解析, 生物物理化学, 56巻, pp. 41-44, 2012
  27. 蛍光性Phos-tagを用いたタンパク質リン酸化反応の解析法, 生物物理化学, 56巻, pp. 45-49, 2012
  28. フォスタグテクノロジーを用いたリン酸化タンパク質解析法, 生物物理化学, 56巻, pp. 51-75, 2012
  29. A novel phosphate-affinity bead immobilized with Phos-tag for separation and enrichment of phosphopeptides and phosphoproteins, Journal of Integrated Omics, 1巻, 1号, pp. 157-169, 2011
  30. A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the kinase reaction of a substrate peptide, ANALYTICAL METHODS, 3巻, 6号, pp. 1303-1309, 2011
  31. Zinc(II)-cyclen polyacrylamide gel electrophoresis for detection of mutations in short Ade/Thy-rich DNA fragments, ANALYTICAL BIOCHEMISTRY, 408巻, 2号, pp. 348-350, 2011
  32. Phos-tagを用いた高分子量リン酸化タンパク質の質的・量的変動モニタリング法, 生化学(日本生化学会機関誌), 82巻, 9号, pp. 857-862, 2010
  33. A clean-up technology for the simultaneous determination of lysophosphatidic acid and sphingosine-1-phosphate by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry using a phosphate-capture molecule, Phos-tag, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 24巻, 7号, pp. 1075-1084, 2010
  34. The DNA-binding activity of mouse DNA methyltransferase 1 is regulated by phosphorylation with casein kinase 1 delta/epsilon, BIOCHEMICAL JOURNAL, 427巻, pp. 489-497, 2010
  35. Dibridged Bis(Zn2+-cyclen): A Novel Host Molecule of Malonate Dianion in Aqueous Solution, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 83巻, 3号, pp. 267-272, 2010
  36. Genotyping and mapping assay of single-nucleotide polymorphisms in CYP3A5 using DNA-binding zinc(II) complexes, CLINICAL BIOCHEMISTRY, 43巻, 3号, pp. 302-306, 2010
  37. Phosphate-affinity Gel Electrophoresis Using a Phos-tag Molecule for Phosphoproteome Study, Current Proteomics, 6巻, pp. 104-121, 2009
  38. Phos-tag beads as an immunoblotting enhancer for selective detection of phosphoproteins in cell lysates, Analytical Biochemistry, 389巻, 1号, pp. 83-85, 2009
  39. Mobility shift detection of phosphorylation on large proteins using a Phos-tag SDS-PAGE gel strengthened with agarose, PROTEOMICS, 9巻, 16号, pp. 4098-4101, 2009
  40. A Phos-tag-based fluorescence resonance energy transfer system for the analysis of the dephosphorylation of phosphopeptides, ANALYTICAL BIOCHEMISTRY, 388巻, 2号, pp. 235-241, 2009
  41. Formation of Lysophosphatidic Acid, a Wound-Healing Lipid, during Digestion of Cabbage Leaves, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 73巻, 6号, pp. 1293-1300, 2009
  42. Cytotoxic 16-beta-[(D-Xylopyranosyl)oxy]oxohexadecanyl triterpene glycosides from a Malagasy plant, Physena sessiliflora, PHYTOCHEMISTRY, 70巻, 9号, pp. 1195-1202, 2009
  43. Phosphate-Affinity Gel Electrophoresis Using a Phos-Tag Molecule for Phosphoproteome Study, CURRENT PROTEOMICS, 6巻, 2号, pp. 104-121, 2009
  44. Two-dimensional phosphate-affinity gel electrophoresis for the analysis of phosphoprotein isotypes, ELECTROPHORESIS, 30巻, 3号, pp. 550-559, 2009
  45. Separation and detection of large phosphoproteins using Phos-tag SDS-PAGE, NATURE PROTOCOLS, 4巻, 10号, pp. 1513-1521, 2009
  46. 細胞内リン酸化タンパク質を高精度に検出するためのPhos-tagビーズによるサンプル前処理法, 細胞工学, 28巻, pp. 934-938, 2009
  47. An Efficient Identification Method of a Specific Binding Protein for a Bioactive Compound Using On-Bead Digestion and Mass Spectrometry, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 81巻, 12号, pp. 1599-1604, 2008
  48. Separation of phosphoprotein isotypes having the same number of phosphate groups using phosphate-affinity SDS-PAGE, PROTEOMICS, 8巻, 15号, pp. 2994-3003, 2008
  49. Detection of the Gua/Cyt-to-Cyt/Gua mutation in a Gua/Cyt-lined sequence using Zn2+-cyclen polyacrylamide gel electrophoresis, ANALYTICAL BIOCHEMISTRY, 380巻, 1号, pp. 122-127, 2008
  50. FANCI phosphorylation functions as a molecular switch to turn on the Fanconi anemia pathway, NATURE STRUCTURAL & MOLECULAR BIOLOGY, 15巻, 11号, pp. 1138-1146, 2008
  51. Differential effects of linear and cyclic polyamines on NMDA receptor activities, NEUROCHEMISTRY INTERNATIONAL, 53巻, 1-2号, pp. 38-44, 2008
  52. A mobility shift detection method for DNA methylation analysis using phosphate affinity polyacrylamide gel electrophoresis, ANALYTICAL BIOCHEMISTRY, 378巻, 1号, pp. 102-104, 2008
  53. Separation of a phosphorylated-His protein using phosphate-affinity polyacrylamide gel electrophoresis, Analytical Biochemistry, 360巻, pp. 160-162, 2007
  54. Non-SCN5A Related Brugada Syndromes: Verification of Normal Splicing and Trafficking of SCN5A without Exonic Mutations, Annals of Human Genetics, 71巻, pp. 8-17, 2007
  55. Identification on membrane and characterization of phosphoproteins using an alkoxide-bridged dinuclear metal complex as a phosphate binding tag molecule, Journal of Biomolecular Techniques, 18巻, 5号, pp. 278-286, 2007
  56. キナーゼプロファイリングのための新しいリン酸アフィニティー電気泳動法, 生物物理化学(日本電気泳動学会編), 51巻, 3号, pp. 199-206, 2007
  57. Specific recognition and detection of phosphorylated proteins using characteristics of metal ion, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 127巻, 12号, pp. 1897-1913, 2007
  58. Label-free kinase profiling using phosphate affinity polyacrylamide gel electrophoresis, MOLECULAR & CELLULAR PROTEOMICS, 6巻, 2号, pp. 356-366, 2007
  59. A single nucleotide polymorphism genotyping method using phosphate-affinity polyacrylamide gel electrophoresis, ANALYTICAL BIOCHEMISTRY, 361巻, 2号, pp. 294-298, 2007
  60. リン酸基親和性クロマトグラフィーを用いた細胞内のリン酸化タンパク質の網羅的精製, バイオテクノロジージャーナル,羊土社, 7巻, pp. 217-220, 2007
  61. リン酸化タンパク質のアフィニティー電気泳動法を利用した細胞内タンパク質リン酸化反応の解析法, 実験医学, 羊土社, 25巻, pp. 1229-1235, 2007
  62. リン酸基アフィニティー電気泳動法を利用した遺伝子診断法, 実験医学, 羊土社, 25巻, pp. 3033-3039, 2007
  63. ★, Phosphate-binding tag, a new tool to visualize phosphorylated proteins, MOLECULAR & CELLULAR PROTEOMICS, 5巻, 4号, pp. 749-757, 2006
  64. Enrichment of phosphorylated proteins from cell lysate using a novel phosphate-affinity chromatography at physiological pH, PROTEOMICS, 6巻, 19号, pp. 5088-5095, 2006
  65. Phos-tagを用いたリン酸化タンパク質の検出法1, 実験医学, 羊土社, 24巻, pp. 2523-2528, 2006
  66. Phos-tagを用いたリン酸化タンパク質の検出法2, 実験医学, 羊土社, 24巻, pp. 2649-2654, 2006
  67. Reliable and cost-effective screening of inherited heterozygosity by Zn2+-cyclen polyacrylamide gel electrophoresis, CLINICAL CHEMISTRY, 51巻, 11号, pp. 2195-2198, 2005
  68. Detection and quantification of on-chip phosphorylated peptides by surface plasmon resonance imaging techniques using a phosphate capture molecule, ANALYTICAL CHEMISTRY, 77巻, 13号, pp. 3979-3985, 2005
  69. An alkoxide-bridged dinuclear zinc(II) hexaazacryptate: A novel phosphate capture molecule in aqueous solution, BULLETIN OF THE CHEMICAL SOCIETY OF JAPAN, 78巻, 1号, pp. 125-131, 2005
  70. Novel immobilized zinc(II) affinity chromatography for phosphopeptides and phosphorylated proteins, JOURNAL OF SEPARATION SCIENCE, 28巻, 2号, pp. 155-162, 2005
  71. A heteroduplex-preferential T-m depressor for the specificity-enhanced DNA polymerase chain reactions, ANALYTICAL BIOCHEMISTRY, 337巻, 1号, pp. 154-160, 2005
  72. Recognition of phosphate monoester dianion by an alkoxide-bridged dinuclear zinc(II) complex, Dalton Transactions, 8号, pp. 1189-1193, 2004
  73. Inhibition of NADP-dependent enzyme activities using the dinuclear zinc chelate, Phos-tag, and reactivation depending on phosphate ion concentrations, J. Anal. Bio-Science, 27巻, 3号, pp. 244-248, 2004
  74. Production of 1,2-didocosahexaenoyl phosphatidylcholine by bonito muscle lysophosphatidylcholine/transacylase, JOURNAL OF BIOCHEMISTRY, 136巻, 4号, pp. 477-483, 2004
  75. Quantitative analysis of lysophosphatidic acid by time-of-flight mass spectrometry using a phosphate-capture molecule, JOURNAL OF LIPID RESEARCH, 45巻, 11号, pp. 2145-2150, 2004
  76. A macrocyclic zinc(II) fluorophore as a detector of apoptosis, Proceedings of the National Academy of Sciences of USA, 100巻, 7号, pp. 3731-3736, 2003
  77. Computer-aided design of a factor Xa inhibitor by using MCSS functionality maps and a CAVEAT linker search, JOURNAL OF MOLECULAR GRAPHICS & MODELLING, 22巻, 2号, pp. 105-114, 2003
  78. A novel antibacterial 8-chloroquinolone with a distorted orientation of the N1-(5-amino-2,4-difluorophenyl) group, JOURNAL OF MEDICINAL CHEMISTRY, 46巻, 10号, pp. 1905-1917, 2003
  79. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of phosphorylated compounds using a novel phosphate capture molecule, RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 17巻, 18号, pp. 2075-2081, 2003
  80. Streptomyces Phospholipase A2: Its Use in the Enzymatic Measurement of Calcium(II) Ion Content, Actinomycetologica, 16巻, 2号, pp. 17-20, 2002
  81. Effects of bis(aromatic) pendants on recognition of nucleobase thymine by zinc(II)-1,4,7,10-tetraazacyclododecane, Supramolecular Chemistry, 14巻, 2号, pp. 95-102, 2002
  82. A novel procedure for simple and efficient genotyping of single nucleotide polymorphisms by using the Zn2+-cyclen complex, Nucleic Acids Research, 30巻, e126号, pp. 1-6, 2002
  83. A novel prokaryotic phospholipase A(2) - Characterization, gene cloning, and solution structure, JOURNAL OF BIOLOGICAL CHEMISTRY, 277巻, 22号, pp. 20051-20058, 2002
  84. Synthesis and characterization of the zinc(II)-fluorophore, 5-dimethylaminonaphthalene-1-sulfonic acid [2-(1,5,9-triazacyclododec-1-yl)ethyl] amide and its zinc(II) complex, JOURNAL OF THE CHEMICAL SOCIETY-DALTON TRANSACTIONS, 8号, pp. 1764-1768, 2002
  85. Novel mechanism of blocking Na channels in squid axon by three macrocyclic polyamine analogues and two spider toxins, British Journal of Pharmacology, 132巻, 1号, pp. 63-72, 2001
  86. The Construction of The Latest Medicinal Information Service System in Medical Field, Japanese Journal of Drug Informatics, 3巻, pp. 133-136, 2001
  87. Selective recognition of consecutive G sequence in double-stranded DNA by a Zinc(II)-macrocyclic tetraamine complex appended with an anthraquinone, Journal of Inorganic Biochemistry, 82巻, pp. 239-249, 2000
  88. Controlling gene expression by zinc(II)-macrocyclic tetraamine complexes, Journal of Inorganic Biochemistry, 79巻, pp. 253-259, 2000
  89. Elaboration of selective and efficient recognition of thymine base in dinucleotides (TpT, ApT, CpT, and GpT), single-stranded d(GTGACGCC), and double-stranded d(CGCTAGCC)(2) by Zn2+-acridinylcyclen (acridinylcyclen = (9-acridinyl)methyl-1,4,7,10-tetraazacyclododecane), JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 122巻, 19号, pp. 4668-4677, 2000
  90. Three-dimensional supermolecules assembled from a tris(Zn2+-cyclen) complex and di- and trianionic cyanuric acid in aqueous solution (cyclen=1,4,7,10-tetraazacyclododecane), JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 122巻, 4号, pp. 576-584, 2000
  91. Novel Recognition of Thymine Base in Double-Stranded DNA by Zinc(II)-Macrocyclic Tetraamine Complexes Appended with Aromatic Groups, Journal of American Chemical Society, 121巻, 23号, pp. 5426-5436, 1999
  92. Dynamic Enolate Recognition in Aqueous Solution by Zinc(II) in a Phenacyl-pendant Cyclen Complex: Implications for the Role of Zinc(II) in Class II Aldolases, Journal of American Chemical Society, 121巻, 6号, pp. 1267-1274, 1999
  93. Selective and Efficient Recognition of Thymidylylthymidine (TpT) by Bis(Zn-cyclen) and Thymidylylthymidylylthymidine (TpTpT) by Tris(Zn-cyclen), Chemistry, A Europian Journal, 5巻, 11号, pp. 3113-3123, 1999
  94. A new macrocyclic tetraamine, 2,4-dinitrophenylcyclen: synthesis, cation reporter properties, and recognition of 1-methylthymine by its zinc(II) complex, Inorganica Chimica Acta, 270巻, pp. 424-432, 1998
  95. Recent development of zinc-fluorophores, Chemical Society Reviews, 27巻, pp. 179-184, 1998
  96. Dynamic anion recognition by macrocyclic polyamines in neutral pH aqueous solution: development from static anion complexes to an enolate complex, Chemical Communications: Feature Article, 1号, pp. 1495-1500, 1998
  97. Structure and O2-Uptake Properties of a Novel Ni(II) Complex of Pyridyl-pendant Dioxocyclam, Journal of Biological and Inorganic Chemistry, 2巻, pp. 74-82, 1997
  98. A Tris(Zinc(II)-1,4,7,10-tetraazacyclododecane) Complex as a New Receptor for Phosphate Dianions in Aqueous Solution, Journal of American Chemical Society, 119巻, 13号, pp. 3068-3076, 1997
  99. Why are Zinc Phosphatases Multinuclear?, J. Synth. Org. Chem., Jpn., 55巻, pp. 1052-1061, 1997
  100. 大環状ポリアミンを用いた人工亜鉛酵素の設計と機能, 化学総説(日本化学会編), 31巻, pp. 25-32, 1997
  101. Facile and Selective, Electrostatic Stabilization of Uracil N(1)-Anion by a Proximate Protonated Amine: A Chemical Implication for Why Uracile N(1) is Chosen for Glycocylation Site, Journal of American Chemical Society, 119巻, 45号, pp. 10909-10919, 1997
  102. 大環状ポリアミン化学の新展開, 現代化学(東京化学同人), 7巻, pp. 31-37, 1997
  103. A New Bis(zinc(II)-cyclen) Complex as A Novel Chelator for Barbiturates and Phosphates, Heterocycles, 42巻, 2号, pp. 775-787, 1996
  104. A New Cyclam with a Triphenylphosphine-pendant and Its Metal Complexes, Inorganica Chimica Acta, 246巻, pp. 151-158, 1996
  105. ★, Novel Properties of Cooperative Dinuclear Zinc(II) Ions: The Selective Recognition of Phosphomonoester and Their P-O Ester Bond Cleavage by New Dinuclear Zinc(II) Cryptate, Journal of American Chemical Society, 118巻, 13号, pp. 3091-3099, 1996
  106. Bis-(zinc(II)-cyclen) Complex as a Novel Receptor of Barbiturates in Aqueous Solution, Chemistry, A Europian Journal, 2巻, 6号, pp. 617-623, 1996
  107. Differntial Contribution of Metal Complexation and Dimerization to the Chemotherapeutic Potential of Bicyclen-Zinc(II) Complex against Human Immunodeficiency Virus, Biological Pharmaceutical Bulletin, 19巻, 3号, pp. 456-458, 1996
  108. Hydrolysis of Lipophilic Esters Catalyzed by a Zinc(II) Complex of a Long Alkyl-Pendant Macrocyclic Tetraamine in Micellar Solution, Journal of American Chemical Society, 118巻, 45号, pp. 10963-10970, 1996
  109. A Novel Biomimetic Zinc(II)-Fluorophore, Dansylamidoethylpendant Macrocyclic Tetraamine 1,4,7,10-Tetraazacyclododecan, Journal of American Chemical Society, 118巻, 50号, pp. 12696-12703, 1996
  110. The Catalytic Carboxyester Hydrolysis by a New Zinc(II) Complex with an Alcohol-Pendant Cyclen (1-(2-Hydroxyethyl)-1,4,7,10-tetraazacyclododecane): A Novel Model for Indirect Activation of the Serine Nucleophile by Zinc(II) in Zinc Enzymes, Journal of American Chemical Society, 117巻, 4号, pp. 1210-1219, 1995
  111. Formation Equilibrium of a Copper(II)-Binuclear Complex of a New Pyridyl-Containing Tetraoxo Octaaza Macrocyclic Ligand, Bulletin Chemical Society of Japan, 68巻, 6号, pp. 1627-1633, 1995
  112. A Zinc(II)-Cyclen Complex Attached to an Anthraquinone Moiety that Acts as a Redox-Active Nucleobase Receptor in Aqueous Solution, Bulletin Chemical Society of Japan, 68巻, 9号, pp. 2465-2469, 1995
  113. Dimeric Macrocyclic Polyamine with Potent Inhibitory Activity against Human Immunodeficiency Virus, Aniviral Chemistry & Chemotherapy, 6巻, 5号, pp. 337-344, 1995
  114. Phosphodiester Hydrolysis by a New Zinc(II) Macrocyclic Tetraamine Complex with an Alcohol Pendant: Elucidation of the Role of Ser-102 and Zinc(II) in Alkaline Phosphatase, Journal of American Chemical Society, 117巻, 32号, pp. 8304-8311, 1995
  115. Inhibition of Human Immunodeficiency Virus Type 1 (HIV-1) Replication by Macrocyclic Polyamines and Their Metal Complexes, Biological and Pharmaceutical Bulletin, 17巻, 2号, pp. 243-250, 1994
  116. Carboxyester Hydrolysis Promoted by a New Zinc(II) Macrocyclic Triamine Complex with an Alkoxide-pendant: A Model Study for the Serine Alkoxide Nucleophile in Zinc Enzyme, Journal of American Chemical Society, 116巻, 11号, pp. 4764-4771, 1994
  117. Chemical Sensing Based on Membrane Potential Change Induced by Host-Guest Complexation at Membrane Surface, Supramolecular Chemistry, 4巻, pp. 101-113, 1994
  118. Role of Zinc(II) in beta-Lactamase II: A Model Study with a Zinc(II)-Macrocyclic Tetraamine (1,4,7,10-Tetraazacyclododecane, Cyclen) Complex, Journal of American Chemical Society, 116巻, 19号, pp. 8443-8449, 1994
  119. The Gold(III)-cyclam Complexes. X-ray Crystal Structure and a Useful Macrocyclic Effect on Reduction of Gold(III) for Development of New Gold Metal Plating, Journal of Coordination Chemistry, 28巻, pp. 33-49, 1993
  120. Novel Bifunctional Macrocyclic Chelating Agents Appended with a Pendant-Type Carboxymethylamino Ligand and Nitrobenzyl Group and Stability of the Y(III) Complexes, Journal of Organic Chemistry, 58巻, 7号, pp. 1955-1958, 1993
  121. A New Cyclam with an NH2-Pendant Donor, 6-Amino-1,4,8,11-tetraazacyclotetradecane, and Its Nickel(II) Complexes, Inorganic Chemistry, 32巻, 12号, pp. 2779-2787, 1993
  122. Novel Pendant-type Macrocyclic Bifunctional Chelating Agents: Carboxymethylamino Derivatives of 2-(4-Nitrobenzyl)-1,4,7,10-tetraazacyclododecane-N,N',N'',N'''-tetraaxetic Acid and Their Complex Formation with Yttrium(III), Journal of Organic Chemistry, 58巻, 24号, pp. 6895-6899, 1993
  123. Potentiometric Discrimination between Planar and Octahedral Anionic Metal Cyano Complexes by Liquid Membranea Electrodes Based on Lipophilic Macrocyclic Dioxopolyamines, Electroanalysis, 5巻, pp. 731-738, 1993
  124. 人工亜鉛酵素の設計と合成, 化学と工業(日本化学会編), 46巻, pp. 244-246, 1993
  125. Kinetics and Mechanism of the Hydration of CO2 and Dehydration of HCO3- Catalyzed by a Zn(II) Complex of 1,5,9-Triazacyclododecane as a Model for Carbonic Anhydrase, Inorganic Chemistry, 32巻, 25号, pp. 5749-5755, 1993
  126. X-ray Structural Study of Zinc(II) Inclusion Complex of a Phenolate-pendant Cyclam, Journal of Inclusion Phenomena Molecular Recognition Chemistry, 12巻, pp. 377-378, 1992
  127. A Versatile Macrocyclic [12]aneN3 for Interconversion of Tetrahedral and Trigonal Bipyramidal Zinc(II) Complexes. Relevance to 4- and 5-Coordinate Geometries of Zinc(II) in Carbonic Anhydrase, Chemistry Letters, pp. 787-790, 1992
  128. The First Anionic Sulfonamide-Binding Zinc(II) Complexes with a Macrocyclic Triamine: Chemical Verification of the Sulfonamide Inhibition of Carbonic Anhydrase, Journal of American Chemical Society, 114巻, 19号, pp. 7338-7345, 1992
  129. Role of Substituents in Copper(II) Extraction with N,N'-Bis(8-quinolyl)malonamides, Journal of Chemical Society, Dalton Transactions, pp. 2679-2683, 1992
  130. Thermodynamic and Kinetic Studies of Lanthanide Complexes of 1,4,7,10,13-Pentaaza-cyclopentadecane-N,N',N'',N''',N''''-pentaacetic Acid and 1,4,7,10,13,16-Hexaazacyclo-octadecane-N,N',N'',N''',N'''',N'''''-Hexaacetic Acid, Inorganic Chemistry, 30巻, 6号, pp. 1270-1273, 1991
  131. ★, Roles of Zinc(II) Ion in Phosphatases. A Model Study with Zinc(II)-Macrocyclic Polyamine Complexes, Journal of American Chemical Society, 113巻, 23号, pp. 8935-8941, 1991
  132. Macrocyclic Polyamines as a Probe for Equilibrium Study of the Acid Function of Zinc(II) Ion in Hydrolysis Enzymes, Comments on Inorganic Chemistry, 11巻, pp. 285-301, 1991
  133. A Study of New Bis(macrocyclic polyamine) Ligands as Inorganic and Organic Anion Recepters, Journal of Organic Chemistry, 55巻, 1号, pp. 42-46, 1990
  134. Comparison of Potentiometric Responce Behavior of Liquid Membranes incorpotrated with N4 and N5 Lipophilic Macrocyclic Polyamines, Bunseki Kagaku, 39巻, pp. 671-676, 1990
  135. A Novel Cyclam Appended with 3-Hydroxypyridine. An Ambident Donor Ligand Comprising a Pyridyl N and a Pyridinolate O-Donors, Inorganic Chemistry, 29巻, 24号, pp. 4991-4996, 1990
  136. A Zinc(II) Complex of 1,5,9,-Triazacyclododecane ([12]aneN3) as a Model for Carbonic Anhydrase, Journal of American Chemical Society, 112巻, 15号, pp. 5805-5811, 1990
  137. Acid Properties of Zn(II) and Cd(II) in Complexation with Macrocyclic Oxo Polyamine Ligands, Inorganic Chemistry, 29巻, 23号, pp. 4621-4629, 1990
  138. Polarographic Study of the Rates of the Dissociation Reactions of the Eu(III)-HAHA Complex and of the Eu(III)-, Gd(III)-, and Ho(III)-PAPA Complexes, Bulletin of Chemical Society of Japan, 63巻, 9号, pp. 2639-2645, 1990
  139. The Electrochemical Study of Ag(II) Complexes of Polyaza Macrocycles and Their Acetic Acid Derivatives, Bulletin of Chemical Society of Japan, 63巻, 10号, pp. 2803-2808, 1990
  140. A Copper(I) N,N,N',N'',N''-Pentamethyldiethylenetriamine Complex and Its Carbon Monoxide Adduct in Aqueous Solutions, Inorganic Chemistry, 28巻, 15号, pp. 2998-3001, 1989
  141. Complexation Reactions of Copper(II) Ions with 16-Membered and 18-Membered Monocyclic Dioxopolyamines in an Aqueous Solution, Bulletin of Chemical Society of Japan, 62巻, 12号, pp. 4044-4046, 1989
  142. Potentiometric Liquid Membrane Sensors That Discriminate Linear Homologs and Geometrical/Positional Isomers of Dicarboxylates, Analitical Letters, 22巻, 5号, pp. 1089-1105, 1989
  143. 機能性大環状ポリアミンの化学, 化学と工業(日本化学会編), 42巻, pp. 280-284, 1989
  144. A Convenient Synthesis of a Macrocyclic Dioxo Pentaamine and X-ray Crystal Structure of Its Monohydrazoic Acid Salt, Journal of Organic Chemistry, 54巻, 16号, pp. 3998-4000, 1989
  145. First X-Ray Crystal Structures of Nickel(II)-Oxocyclam Complexes. Effects of the Deprotonated Amide and of an Intramolecular Pendant Pyridine on the Cyclam Ligand Field, Inorganic Chemistry, 27巻, 6号, pp. 1036-1040, 1988
  146. A Trigonal Bipyramidal Zinc(II) Complex of Phenol-pendant Macrocyclic Triamine, Inorganic Chemistry, 27巻, 20号, pp. 3687-3688, 1988
  147. Potentiometric Adenosine Triphosphate Polyanion Sensor Using a Lipophilic Macrocyclic Polyamine Liquid Membrane, Analytical Chemistry, 60巻, 21号, pp. 2392-2396, 1988
  148. The o-Methoxyphenol-Pendant Cyclam Complexes. A Novel Molecule Designed for Intramolecular Redox Coupling between Monodentate Catecholate and Metal Ions in an N4 Macrocycle, Journal of American Chemical Society, 109巻, pp. 5528-5529, 1987
  149. New-Dimensional Cyclam. Synthesis, Crystal Structure, and Chemical Properties of Macrocyclic Tetraamines Bearing a Phenol Pendant, Inorganic Chemistry, 26巻, 18号, pp. 2975-2983, 1987
  150. A New Mg(II) Ion Receptor. Macrocyclic Polyamines Bearing an Intraannular Phenolic Group, Journal of American Chemical Society, 109巻, pp. 6212-6213, 1987
  151. A potent superoxide dismutase-like activity by cimetidine-copper complex, Free Radicals in Clinical Medicine, 1巻, pp. 79-81, 1987
  152. Molecular Structure of a Nickel(II) Complex with Phenolate-appended Cyclam, Inorganic Chemistry, 25巻, 4号, pp. 402-404, 1986
  153. Complexes of the Histamine H2-Antagonist Cimetidine with Divalent and Monovalent Copper Ions, Inorganic Chemisty, 25巻, 13号, pp. 2242-2246, 1986
  154. Crystal and Solution Structures of Nickel(II)-13-Membered Macrocyclic Tetramine [1,4,7,10-Tetraazacyclotridecane] Effected by a Pendant Phenol, Chemistry Letters, 15巻, pp. 1137-1140, 1986
  155. Novel Electrochemical Behaviour of a Nickel(II)-13-Membered Macrocyclic Tetra-amine effected by a Pendant Phenol, Journal of Chemical Society, Chemical Communications, pp. 1110-1111, 1986
  156. A New Cyclam Providing the First Example Unequivocally Proven by X-Ray Crystallography of a Pendent Co-ordinated Pyridyl Group, Journal of Chemical Society, Chemical Communications, pp. 1322-1323, 1986
  157. Synthesis and Metal Complexes of Macrocyclic Triamines Bearing a Phenol Pendant, Inorganic Chemistry, 25巻, 22号, pp. 3883-3886, 1986
  158. Study on Pharmaceutical Chemistry of Biomimetic Metal Complexes, Doctoral Thesis (Hiroshima University), pp. 1-65, 1986
  159. Novel Synthesis and Complex Properties of Macrocyclic Tetraamines Appended with Phenol as an Axial Donor, Journal of Chemical Society, Chemical Communications, pp. 385-386, 1985
  160. Rapid Oxidative Metal Insertion into a Macrocyclic Ring from a ’Metal-out’ Position and Identification of a Stable ’Metal-in’ Isomer of a Ni(II)-Cyclamtetra-acetate Complex, Journal of Chemical Society, Chemical Communications, pp. 1341-1343, 1985
  161. Metal Chelates of Sulphur-containing Polyamine Macrocycles and Oxygenation of the Corresponding Cobalt(II) Complexes, Journal of Chemical Society, Dalton Transactions, pp. 673-678, 1984
  162. Effects of Imide Anions and Axial Donors on the Stability and Oxidation Behavior of Square Planar 13-15-Membered Macrocyclic Tetraamine Complexes of Nickel(II) and Copper(II), Inorganic Chemistry, 23巻, 25号, pp. 4181-4188, 1984
  163. Macrocyclic Polyamines as a Possible Chemical Model for Histamine H2-Receptors, Chemical and Pharmaceutical Bulletin, 32巻, 9号, pp. 3569-3578, 1984
  164. Further Studies on Superoxide Dismutase Activities of Macrocyclic Polyamine Complexes of Copper(II), Biochimica Biophysica Acta, 745巻, pp. 37-43, 1983
  165. A novel thiol-affinity micropipette tip method using zinc(II)-cyclen-attached agarose beads for enrichment of cysteine-containing molecules, JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 1031巻, pp. 195-201, 20160915
  166. A novel thiol-affinity micropipette tip method using zinc(II)–cyclen-attached agarose beads for enrichment of cysteine-containing molecules, Journal of Chromatography B, 1031巻, pp. 195-201, 2016
  167. Specific glutamic acid residues in targeted proteins induce exaggerated retardations in Phos-tag SDS-PAGE migration, ELECTROPHORESIS, 38巻, 8号, pp. 1139-1146, 201704
  168. TAMRA/TAMRA Fluorescence Quenching Systems for the Activity Assay of Alkaline Phosphatase, SENSORS, 17巻, 8号, 201708
  169. A Phos-tag-based micropipette-tip method for rapid and selective enrichment of phosphopeptides, ELECTROPHORESIS, 38巻, 19号, pp. 2447-2455, 201710
  170. Phos-tag SDS-PAGE methodology that effectively uses phosphoproteomic data for profiling the phosphorylation dynamics of MEK1, Journal of Electrophoresis, 61巻, pp. 9-15, 20170107
  171. A simple method for determining the ligand affinity toward a zinc-enzyme model by using a TAMRA/TAMRA interaction, DALTON TRANSACTIONS, 47巻, 6号, pp. 1841-1848, 20180214
  172. Increase in constitutively active MEK1 species by introduction of MEK1 mutations identified in cancers, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1867巻, 1号, pp. 62-70, 201901
  173. A method for profiling the phosphorylation state of tyrosine protein kinases, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1867巻, 1号, pp. 71-75, 201901
  174. Gel-based analysis of protein phosphorylation status by rapid fluorometric staining using TAMRA-labeled Phos-tag, Journal of Electrophoresis, 63巻, 1号, pp. 25-32, 20190401

著書等出版物

  1. 2016年, Phospho-Proteomics – Methods and Protocols: Methods in Molecular Biology, Edited by Louise von Stechow: Phosphopeptide Detection with Biotin-Labeled Phos-tag, 2016年, 単行本(学術書), 共著, doi: 10.1007/978-1-4939-3049-4_2, 13
  2. 2016年, Macrocyclic and Supramolecular Chemistry: How Izatt-Christensen Award Winners Shaped the Field, Evolution of Zinc(II)–Macrocyclic Polyamines to Biological Probes and Supramolecular Assembly, 2016年, 07, 単行本(学術書), 共著, English, 木村栄一、小池透、青木伸, ISBN 998-1-119-05384-2
  3. 2015年, Proteomic Profiling: Methods in Molecular Biology, Edited by Anton Posch, Chapter 24: Neutral Phosphate-Affinity SDS-PAGE System for Profiling of Protein Phosphorylation, 2015年, 単行本(学術書), 共著, doi: 10.1007/978-1-4939-2550-6_24 ISBN: 978-1-4939-2549-0
  4. 2015年, Kinomics: Approaches and Applications, Edited by H. Kraatz and S. Martic: Phos-tag Technology for Kinomics, 2015年, 単行本(学術書), 共著, E. Kinoshita-Kikuta, E. Kinoshita, T. Koike, ISBN: 978-3-527-33765-1
  5. 2012年, リン酸基アフィニティ電気泳動法(Phos-tag SDS-PAGE)を用いたタンパク質リン酸化修飾の高感度検出.実験医学,30,増刊号「シグナル伝達研究最前線2012」, 羊土社, 2012年, 03, 単行本(学術書), 共著, 木下英司, 木下恵美子,小池 透, ISBN: 978-4-7581-0321-3
  6. 2012年, DNA Replication and Mutation: Gel-based methods using DNA-binding zinc(II) complexes for the detection of DNA mutations, Nova Science Publishers, 2012年, 03, 単行本(学術書), 共著, ISBN: 978-1-61324-490-6
  7. 2012年, Phos-tag ゲルによるリン酸化タンパク質の分離・同定法.臨床プロテオミクス~バイオマーカー探索から個別化医療へ, 金原出版, 2012年, 05, 単行本(学術書), 共著, 木下英司,木下恵美子,小池 透, ISBN: 978-4-307-00470-1, 4
  8. 2012年, Protein Kinase Technologies, Neuromethods: Phos-tag Affinity Electrophoresis for Protein Kinase Profiling, 2012年, ISBN: 978-1-61779-823-8
  9. 2010年, やさしい物理化学 -自然を楽しむための12講-, 共立出版株式会社, 2010年, 09, 教科書, 単著, 小池 透, ISBN: 978-4-320-04394-7, 163
  10. 2010年, Single Nucleotide Polymorphisms Methods and Protocols Second Edition: "Zn(II)-Cyclen Polyacrylamide Gel Electrophoresis for SNP Detection ", 2010年, ISBN: 978-1-60327-410-4
  11. 2007年, 新しい化学実験カリキュラム「化学実験書」 , 広島県立広島国泰寺高校,SSHコンテンツ, 2007年, 03, 教科書, 共著, 小池 透, 柞磨昭孝, 落合節子, 高須泰仁, 46
  12. 2004年, Encyclopedia of Supramolecular Chemistry: Carbonic Anhydrase Models , Taylor & Francis, 2004年, 04, 単行本(学術書), 共編著, Tohru Koike, Eiichi Kimura, ISBN: 978-0824750565, 5
  13. 1999年, Bioinorganic Catalysis, 2nd Edition: Lewis Acid Properties of Zinc and Its Development to Phosphotriester Detoxifying Agents, Marcel Dekker, New York, 1999年, 04, 単行本(学術書), 共編著, ISBN: 0-8247-0241-7
  14. 1999年, Prespective on Bioinorganic Chemistry: "Macrocyclic Polyamines and Their Metal Complexes: A Novel Type of Anti-HIV Agent", JAI Press Inc., 1999年, 04, 単行本(学術書), 共編著, Eiichi Kimura, Tohru Koike, Yoshio Inouye, ISBN: 0-7623-0352-2
  15. 1997年, Advances in Inorganic Chemistry: "Intrinsic Properties of Zinc(II) Ion Pertinent to Zinc Enzyme", Academic Press, New York, 1997年, 04, 単行本(学術書), 共編著, Eiichi Kimura, Tohru Koike, ISBN: 978-0-12-023644-2
  16. 1997年, Structure and Bonding: Advances in Zinc Enzyme Models by Small, Mononuclear Zinc(II) Complexes , Springer, Berlin, 1997年, 04, 単行本(学術書), 共編著, ISBN: 978-3-540-62874-3, 28
  17. 1996年, Comperhensive Supramolecular Chemistry: Metalloenzyme Models that Answer Mysteries Surrounding the Instrinsic Properties of Zinc(II) , Pregamon, Exeter, 1996年, 04, 単行本(学術書), 共編著, Eiichi Kimura, Tohru Koike, ISBN: 9780080406107, 16
  18. 1988年, 生体分子の化学, 廣川書店, 1988年, 07, 単行本(学術書), 共編著, ISBN: 4-567-24041-3, 16
  19. 2015年, "Protein Modifications in Pathogenic Dysregulation of Signaling, Edited by J. Inoue and M. Takekawa: "Phos-tag-Based Affinity Chromatography Techniques for Enrichment of the Phosphoproteome", 単行本(学術書), 共著, ISBN: 978-4-431-55560-5 doi: 10.1007/978-4-431-55561-2_2
  20. 2010年, Single Nucleotide Polymorphisms Methods and Protocols Second Edition: Phosphate-Affinity Polyacrylamide Gel Electrophoresis for SNP Genotyping, ISBN: 978-1-60327-410-4

招待講演、口頭・ポスター発表等

  1. Thio-tag Tipを用いたチオール基含有化合物の選択的分離精製法, 草本 寛,藤岡晴人,是竹紀尚,木下恵美子,木下英司,小池 透, 日本薬学会第136年会, 2016年03月27日, 通常, 日本語, 横浜
  2. Development of Thio-tag magnetic bead for rapid and selective separation of thiol-containing molecules, Maho Kawaguchi, Norinao Koretake, Emiko Kinoshita-Kikuta, Eiji Kinoshita, and Tohru Koike, 環太平洋国際化学会議2015, 2015年12月15日, 通常, 英語, ホノルル
  3. ハイブリッドセンサーキナーゼのリン酸基リレー情報伝達機構におけるレシーバードメインの制御機能, 木下恵美子,木下英司,江口陽子,吉多美祐,山本兼由,内海龍太郎,小池 透, 第38回日本分子生物学会年会, 2015年12月02日, 通常, 英語, 神戸
  4. 抗がん剤を処理したヒト白血病細胞HL-60の細胞内キナーゼの発現およびリン酸化動態の解析, 杉山康憲,片山将一,亀下 勇,森澤啓子,樋口琢磨,戸高 寛,木下英司,木下恵美子,小池 透,坂本修士, 第38回日本分子生物学会年会, 2015年12月02日, 通常, 英語, 神戸
  5. リン酸基捕捉能を持つダーククエンチャーを用いた蛍光分析法, 國貞夏実,木下恵美子,木下英司,小池 透, 第66回日本電気泳動学会総会, 2015年09月04日, 通常, 日本語

受賞

  1. 2014年, 広島大学 学長表彰, 広島大学

取得

  1. 特許権, 特許4616176, 表面プラズモン共鳴の測定方法および該方法に使用される貴金属化合物
  2. 特許権, 特許4167296, 電気泳動用ポリアクリルアミドゲル,それを用いたポリアクリルアミドゲル電気泳動方法,その製造方法,およびアクリルアミド化合物
  3. 特許権, 特許3972194, 電気泳動用試薬,電気泳動用組成物及び核酸の分離方法
  4. 特許権, 特許4109621, リン酸モノエステル化合物の分子量を求める方法,およびマススペクトル測定用添加剤
  5. 特許権, 特許4266377, アニオン性置換基を有する物質の捕捉剤
  6. 商標権, 登録番号:第4658619号, フォスタグ
  7. 特許権, 特許4403020, 電気泳動用ポリアクリルアミドゲル,それを用いたポリアクリルアミドゲル電気泳動方法,その製造方法,およびアクリルアミド化合物
  8. 特許権, 特許4616194, ビオチン化合物
  9. 特許権, 特許4303595, アニオン性置換基を有する物質を捕捉可能な亜鉛錯体
  10. 特許権, 中国:NoZL2004800299906, 表面プラズモン共鳴の測定方法および該方法に使用される貴金属化合物
  11. 特許権, AU: 2005335179, Polyacrylamide gel for electrophoresis, Polyacrylamide gel electrophoresis method using the same, method of producing the same and acrylamide compound
  12. 特許権, CA: 2,517,705, Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
  13. 特許権, CA: 2617680, Polyacrylamide gel for electrophoresis, Polyacrylamide gel electrophoresis method using the same, method of producing the same and acrylamide compound
  14. 特許権, US: 7,901,559, Polyacrylamide gel for electrophoresis, Polyacrylamide gel electrophoresis method using the same, method of producing the same and acrylamide compound
  15. 特許権, EP: 1911776, Polyacrylamide gel for electrophoresis, Polyacrylamide gel electrophoresis method using the same, method of producing the same and acrylamide compound
  16. 特許権, 台湾:I334869, 表面プラズモン共鳴の測定方法および該方法に使用される貴金属化合物
  17. 特許権, RU: 2356033, Method for Measuring a Surface Plasmon Resonance and Noble Metal Compound Used for the Same
  18. 特許権, US: 7473781, A capturing agent for a substance having an anionic substituent
  19. 特許権, US: 7,358,363, Zinc complexes capable of capturing substances having anionic substituents
  20. 特許権, US: 7,547,784, Zinc complexes capable of capturing substances having anionic substituents
  21. 特許権, DE: 10297577, Zinkkomplexe, die fahig sind, Substanzen, die anionische Substituenten haben, abzufangen
  22. 特許権, US: 7,202,093, Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
  23. 特許権, EP: 1455189, Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
  24. 特許権, 中国:ZL2004 1 0007984.4, リン酸化ペプチドの標識方法および選択的吸着方法、該方法に使用される錯体化合物、該錯体化合物の製造方法、および該錯体化合物の原料化合物
  25. 特許権, 台湾:I318688, リン酸化ペプチドの標識方法および選択的吸着方法、該方法に使用される錯体化合物、該錯体化合物の製造方法、および該錯体化合物の原料化合物
  26. 特許権, RU: 2315771, Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
  27. 特許権, EP: 1602923, METHOD OF MEASURING THE MOLECULAR WEIGHT OF PHOSPHORIC ACID MONOESTER COMPOUND AND AN ADDITIVE FOR MASS MEASUREMENT
  28. 特許権, AU: 2004218127, Method for labeling phosphorylated peptides, method for selectively adsorbing phosphorylated peptides, complex compounds used in the methods, process for producing the complex compounds, and raw material compounds for the complex compounds
  29. 特許権, 中国:ZL200480005857.7, 帯有陰離子性取代基物質的捕捉剤
  30. 特許権, RU: 2326377, METHOD FOR IDENTIFYING MOLECULAR WEIGHT OF A PHOSPHORIC ACID MONOESTER COMPOUND AND AN ADDITIVE FOR MASS SPECTROMETRY
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