鈴木 克周Katsunori Suzuki

Last Updated :2019/07/01

所属・職名
大学院統合生命科学研究科 理学・植物分子細胞構築学(生物科学) 教授
メールアドレス
ksuzukihiroshima-u.ac.jp
その他連絡先
東広島市鏡山一丁目3番1号 理学研究科 A棟理学研究科 A410
TEL:0824-24-7455 FAX:0824-24-0734
自己紹介
細菌が真核生物に遺伝子を輸送する現象について研究しています。    例えば、アグロバクテリアという細菌は植物に遺伝子を注入する能力を持っています。私達の生活に身近な現象では、大豆やトウモロコシなどの遺伝子組換え植物はアグロバクテリアが植物に遺伝子を注入する性質を利用して作られます。バラなどを栽培する人にとってアグロバクテリアは、根瘤を作る病原菌として知られています。有用菌・有害菌として重要であるだけでなく、基礎生物学的にも興味深い特徴をいくつも持っています。    あなたも研究に挑戦しませんか?

基本情報

主な職歴

  • 2004年01月01日, 広島大学, 大学院理学研究科, 教授
  • 1987年04月01日, 通商産業省, 工業技術院 化学技術研究所, 技官

学歴

  • 名古屋大学, 理学研究科, 生物学, 日本, 1982年04月, 1985年03月

学位

  • 理学博士 (名古屋大学)
  • 農学修士 (名古屋大学)
  • 農学士 (名古屋大学)

教育担当

  • 【学士課程】 理学部 : 生物科学科
  • 【博士課程前期】 統合生命科学研究科 : 統合生命科学専攻 : 基礎生物学プログラム
  • 【博士課程後期】 統合生命科学研究科 : 統合生命科学専攻 : 基礎生物学プログラム

研究分野

  • 農学 / 生産環境農学 / 遺伝育種科学
  • 農学 / 農芸化学 / 応用微生物学

研究キーワード

  • 植物ガン他Tiプラスミド|DNA
  • プラスミド|遺伝子
  • Protein
  • 高分子輸送
  • 遺伝子
  • 超生物界/ 生物ドメイン
  • 線状染色体DNA
  • 接合伝達

所属学会

  • 日本分子生物学会
  • 日本植物学会
  • 中国四国植物学会
  • 日本遺伝学会

教育活動

授業担当

  1. 2019年, 教養教育, 3ターム, 生物学入門
  2. 2019年, 学部専門, 4ターム, 生物科学概説B
  3. 2019年, 教養教育, 2ターム, 教養ゼミ
  4. 2019年, 学部専門, 2ターム, 先端生物学
  5. 2019年, 学部専門, 2ターム, 基礎生物科学B
  6. 2019年, 学部専門, 2ターム, 微生物学
  7. 2019年, 学部専門, セメスター(後期), 植物分子細胞構築学演習
  8. 2019年, 学部専門, セメスター(前期), 卒業研究
  9. 2019年, 学部専門, セメスター(後期), 卒業研究
  10. 2019年, 学部専門, セメスター(後期), 生物科学基礎実験IV
  11. 2019年, 学部専門, 4ターム, 生物科学特別講義(細胞内共生進化と遺伝子の水平転移)
  12. 2019年, 博士課程・博士課程後期, セメスター(前期), 植物分子細胞構築学演習
  13. 2019年, 博士課程・博士課程後期, セメスター(後期), 植物分子細胞構築学演習
  14. 2019年, 修士課程・博士課程前期, セメスター(前期), 生物科学特別研究
  15. 2019年, 修士課程・博士課程前期, セメスター(後期), 生物科学特別研究
  16. 2019年, 博士課程・博士課程後期, セメスター(前期), 生物科学特別研究
  17. 2019年, 博士課程・博士課程後期, セメスター(後期), 生物科学特別研究
  18. 2019年, 修士課程・博士課程前期, 年度, 基礎生物学特別研究
  19. 2019年, 修士課程・博士課程前期, 1ターム, 基礎生物学特別演習A
  20. 2019年, 修士課程・博士課程前期, 2ターム, 基礎生物学特別演習A
  21. 2019年, 修士課程・博士課程前期, 3ターム, 基礎生物学特別演習B
  22. 2019年, 修士課程・博士課程前期, 4ターム, 基礎生物学特別演習B
  23. 2019年, 修士課程・博士課程前期, 4ターム, 統合生殖科学特論
  24. 2019年, 博士課程・博士課程後期, 年度, 統合生命科学特別研究

研究活動

学術論文(★は代表的な論文)

  1. Successful transfer of a model T-DNA plasmid to E. coli revealed its dependence on recipient RecA and the preference of VirD2 relaxase for eukaryotes rather than bacteria as recipients, FRONTIERS IN MICROBIOLOGY, 9:895巻, 2018
  2. Effective removal of a range of Ti/Ri plasmids using a pBBR1-type vector having a repABC operon and a lux reporter system, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 102巻, 4号, pp.1823-pp.1836, 201802
  3. An extra repABC locus in the incRh2 Ti plasmid pTiBo542 exerts incompatibility toward an incRh1 plasmid, PLASMID, 90巻, pp.20-pp.29, 201703
  4. A Fast and Practical Yeast Transformation Method Mediated by Escherichia coli Based on a Trans-Kingdom Conjugal Transfer System: Just Mix Two Cultures and Wait One Hour, PLOS ONE, 11巻, 2号, 20160205
  5. DNA repair genes RAD52 and SRS2, a cell wall synthesis regulator gene SMI1, and the membrane sterol synthesis scaffold gene ERG28 are important in efficient Agrobacterium-mediated yeast transformation with chromosomal T-DNA, BMC MICROBIOLOGY, 16巻, 20160402
  6. ★, Horizontal DNA transfer from bacteria to eukaryotes and a lesson from experimental transfers, RESEARCH IN MICROBIOLOGY, 166巻, 10号, pp.753-pp.763, 201512
  7. Transkingdom Genetic Transfer from Escherichia coli to Saccharomyces cerevisiae as a Simple Gene Introduction Tool, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79巻, 14号, pp.4393-pp.4400, 201307
  8. Trans-Kingdom Horizontal DNA Transfer from Bacteria to Yeast Is Highly Plastic Due to Natural Polymorphisms in Auxiliary Nonessential Recipient Genes, PLOS ONE, 8巻, 9号, 20130913
  9. ★, Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon, MICROBIOLOGICAL RESEARCH, 167巻, 5号, pp.311-pp.316, 2012
  10. Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmid, GENES TO CELLS, 17巻, 7号, pp.597-pp.610, 201207
  11. Agrobacterium tumefaciens Tumor Morphology Root Plastid Localization and Preferential Usage of Hydroxylated Prenyl Donor Is Important for Efficient Gall Formation, PLANT PHYSIOLOGY, 159巻, 3号, pp.1064-pp.1072, 201207
  12. Screening for yeast mutants defective in recipient ability for transkingdom conjugation with Escherichia coli revealed importance of vacuolar ATPase activity in the horizontal DNA transfer phenomenon., MICROBIOLOGICAL RESEARCH, 167巻, 5号, pp.311-pp.316, 2012
  13. Development of a reinforced Ti-eviction plasmid useful for construction of Ti plasmid-free Agrobacterium strains, JOURNAL OF MICROBIOLOGICAL METHODS, 89巻, 1号, pp.53-pp.56, 2012
  14. Yeast transformation mediated by Agrobacterium strains harboring an Ri plasmid: comparative study between GALLS of an Ri plasmid and virE of a Ti plasmid, GENES TO CELLS, 17巻, 7号, pp.597-pp.610, 2012
  15. Construction of disarmed Ti plasmids transferable between Escherichia coli and Agrobacterium species, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 75巻, pp.1845-pp.1851, 20090401
  16. Ability of Agrobacterium tumefaciens and A. rhizogenes strains, inability of A. vitis and A. rubi strains to adapt to salt-insufficient environment, and taxonomic significance of a simple salt requirement test in the pathogenic Agrobacterium sepecies, Journal of General and Applied Microbiology, 55巻, pp.35-pp.41, 20090101
  17. Variation of lipopolysaccharide among the three major Agrobacterium species and the effect of environmental stress on the lipopolysaccharide profile, Palnt Pathology Journal, 8巻, pp.1-pp.8, 20090101
  18. ★, Novel toxin-antitoxin system composed of serine protease and AAA-ATPase homologues determines the high level of stability and incompatibility of the tumor-inducing plasmid pTiC58, JOURNAL OF BACTERIOLOGY, 191巻, pp.4656-pp.4666, 20090401
  19. Variation of 16S-23S internally transcribed spacer sequence and intervening sequence in rDNA among the three major Agrobacterium species, MICROBIOLOGICAL RESEARCH, 164巻, 6号, pp.604-pp.612, 2009
  20. Novel toxin-antitoxin system composed of serine protease and AAA-ATPase homologues determines the high level of stability and incompatibility of the tumor-inducing plasmid pTiC58, Journal of Bacteriology, 191巻, 14号, pp.4656-pp.4666, 2009
  21. Identification of pTi-SAKURA DNA region conferring enhancement of plasmid incompatibility and stability, GENES & GENETIC SYSTEMS, 82巻, pp.197-pp.206, 20070101
  22. Variation of 16S-23S internally transcribed spacer sequence and intervening sequence in rDNA among the three major Agrobacterium species, MICROBIOLOGICAL RESEARCH, 164巻, pp.604-pp.612
  23. Construction of physical map and mapping of chromosomal virulence genes of the biovar 3 Agrobacterium (Rhizobium vitis) strain K-Ag-1, Genes Genet. Syst., 81巻, pp.373-pp.380, 20060101
  24. Precise characterization of rDNA genes by intraspecies and inter-loci comparison of rDNA sequences and biochemical analysis of ribosomal RNA molecules in Agrobacterium tumefaciens, GENES & GENETIC SYSTEMS, 80巻, pp.9-pp.17, 20050101
  25. Genome structure and evolution of giant plant pathogenic plasmids in Agrobacterium tumefaciens and Agrobacterium rhizogenes, Endocytobiosis Cell Res., 15巻, pp.371-pp.378, 20040401
  26. Gene search in the left region of the linear chromosome of Agrobacterium tumefaciens MAFF301001, Endocytobiosis Cell Res., 15巻, pp.379-pp.384, 20040401
  27. ★, An overview of the agrobacterial genome; the hetero-trimer constitution by tumor inducing plasmid, linear and circular chromosomal DNAs, Endocytobiosis and Cell Research, 15巻, pp.143-pp.150, 20040101
  28. Characterization of telomere DNA among five species of pteridophytes and bryophytes, JOURNAL OF BRYOLOGY, 26巻, pp.175-pp.180, 2004
  29. Structural and functional analysis of a putative gene cluster for palatinose transport on the linear chromosome of Agrobacterium tumefaciens MAFF301001, JOURNAL OF BACTERIOLOGY, 185巻, pp.2369-pp.2373, 20030401
  30. Physical and gene maps of Agrobacterium biovar 2 strains and their unique relationship to biovar 1 chromosomes, MICROBIOLOGY, 149巻, pp.3035-pp.3042, 20030401
  31. Roles of sexual cell agglutination in yeast mass mating, GENES & GENETIC SYSTEMS, 78巻, 3号, pp.211-pp.219, 200306
  32. Physical and gene maps of Agrobacterium biovar 2 strains and their relationship to biovar 1 chromosomes, MICROBIOLOGY-SGM, 149巻, pp.3035-pp.3042, 200310
  33. ★, Roles of sexual cell agglutination in yeast mass mating, GENES & GENETIC SYSTEMS, 78巻, 3号, 2003
  34. 組み換え植物を作る生物 アグロバクテリアのプラスミドと染色体, アイソトープ総合センターニュース, 7号, pp.15-pp.19, 20020301
  35. A novel curing method by incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens, GENES & GENETIC SYSTEMS, 77巻, pp.1-pp.9, 20020401
  36. Gene cluster for ferric iron uptake in Agrobacterium tumefaciens MAFF301001, GENES & GENETIC SYSTEMS, 77巻, pp.137-pp.145, 20020401
  37. A novel plasmid curing method using incompatibility of plant pathogenic Ti plasmids in Agrobacterium tumefaciens, GENES & GENETIC SYSTEMS, 77巻, 1号, pp.1-pp.9, 200202
  38. Characterization of four ribosomal RNA operons in the genome of Agrobacterium tumefaciens MAFF301001., Nucleic acids research. Supplement (2001), 2号, 2002
  39. ★, Genome analysis of Agrobacterium tumefaciens; construction of physical maps for linear and circular chromosomal DNAs, determination of copy number ratio and mapping of chromosomal virulence genes, DNA Research, 8巻, pp.141-pp.152, 20010401
  40. Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA, Genes and Genetic Systems, 76巻, pp.121-pp.130, 20010401
  41. Genome analysis of Agrobacterium tumefacien, Genes and Genetic Systems, 76巻, pp.363-pp.371, 20010401
  42. コンピュータが活躍するDNA解析, I/O, 26巻, 3号, pp.112-pp.115, 20010301
  43. Sequence characterization of the vir region of a nopaline type Ti plasmid, pTi-SAKURA, GENES & GENETIC SYSTEMS, 76巻, 2号, pp.121-pp.130, 200104
  44. Genome analysis of Agrobacterium tumefaciens: Linkage map and genetic features of the left region of the linear chromosome, GENES & GENETIC SYSTEMS, 76巻, 6号, pp.363-pp.371, 200112
  45. Gene list on a plant tumor-inducing plasmid, pTi-SAKURA in Agrobacterium tumefaciens, DNA Research, 7巻, pp.371-pp.380, 20000401
  46. ★, Complete nucleotide sequence of a plant tumor inducing Ti plasmid, Gene, 242巻, pp.331-pp.336, 20000401
  47. Human lysozyme secretion increased by alpha-factor pro-sequence in Pichia pastoris, Biosciences Biotechnology and Biochemistry, 63巻, pp.1977-pp.1983, 19990401
  48. Novel structural difference between nopaline- and octopine- type trbJ gene; construction of genetic and physical map and sequencing of trb/traI and rep gene clusters of a new Ti plasmid pTi-SAKURA, Biochimica et Biophysica Acta, 1396巻, pp.1-pp.7, 19980401
  49. Nuclear accumulation of Saccharomyces cerevisiae Mcm3 is dependent on its nuclear localization sequence, Genes to Cells, 2巻, pp.631-pp.643, 19970401
  50. The structure and characteristics of Japanese-born Ti-plasmid, Acta Anatomica, 11巻, pp.976-pp.977, 19970401
  51. Restriction enzymes-resistant high molecular weight telomeric DNA fragments in tobacco, DNA Research, 1巻, pp.129-pp.139, 19940401
  52. 生物科学分野におけるネットワークサービス及び汎用高性能計算機利用普及の試み, 情報処理教育研究集会 講演論文集, 19940101
  53. Nucleotide sequences and secondary structures of four tRNA genes in mitochondrial DNA of Hansenula wingei yeast, Nucleic Acids Research, 21巻, pp.3589-3589, 19930401
  54. ★, DNA integration into recipient yeast chromosomes by the transkingdom conjugation between Escherichia coli and Saccharomyces cerevisiae, Cuurent Genetics, 21巻, pp.101-pp.108, 19920401
  55. Nucleotide sequences of ten mitochondorial tRNA genes in yeast Hansenula wingei, Nucleic Acids Research, 20巻, pp.2373-2373, 19920401
  56. Isolation of new temperature sensitive mutants of Saccharomyces cerevisiae deficient in mannose outer chain elongation, Yeast, 8巻, pp.535-pp.547, 19920401
  57. Physical mapping and RFLP analysis of mt-DNAs from the ascosporogenous yeasts Saccharomyces exiguus, S. kluyveri and Hansenula wingei, The Japanese journal of genetics, 66巻, pp.709-pp.718, 19910401
  58. Structural and functional stability of IncP plasmids during stepwise transmission by trans-kingdom matin, 遺伝学雑誌, 65巻, pp.323-pp.334, 19900401
  59. Yeast mutants with enhanced ability to secrete human lysozyme; isolation and identification of a protease-deficient mutant, Molecular and General Genetics, 219巻, pp.58-pp.64, 19890401
  60. The effects of culture conditions on the secretion of human lysozyme by Saccharomyces cerevisiae A2-1-1A, Agricultural and Biological Chemistry, 53巻, pp.2687-pp.2694, 19890401
  61. Metabolism of p-hydroxybenzoate via hydroxyquinol by Trichosporon cutaneum WY2-, Plant and Cell Physiology, 27巻, pp.1451-pp.1460, 19860401
  62. Stepwise transformation in Saccharomyces cerevisiae yeas, Plant and Cell Physiology, 27巻, pp.801-pp.808, 19860401
  63. A mutation leading to constitutive expression of high sexual activities in Saccharomyces cerevisiae, Plant and Cell Physiology, 27巻, pp.523-pp.531, 19860401
  64. Genetic characterization of an alpha-specific gene responsible for sexual agglutinability in Saccharomyces cerevisiae, Current Genetics, 10巻, pp.353-pp.357, 19860401
  65. An alpha-mating-type-specific mutation causing specific defect in sexual agglutinability in the yeast Saccharomyces cerevisiae, Current Genetics, 9巻, pp.185-pp.189, 19850401
  66. 生化学Lab-bench用プログラム:ハンドヘルドコンピュータ簡易データベース用ソフト, 生化学, 56巻, pp.331-pp.337, 19840401
  67. An overview of the Agrobacterial genome, Endocytobiosis Cell Research, 15巻, pp.143-pp.150
  68. Genome structure and evolution of giant plant pathogenic plasmids in Agrobacterium tumefaciens and Agrobacterium rhizogenes, Endocytobiosis Cell Research, 15巻, pp.371-pp.378
  69. Gene search in the left region of the linear chromosome of Agrobacterium tumefaciens MAFF301001, Endocytobiosis Cell Research, 15巻, pp.379-pp.384

著書等出版物

  1. 2009年01月, Microbial Megaplasmids. Schwartz, E. (ed.) in Microbiology Monograph series, Springer Verlag, Heidelberg.pp.133-147, Ti and Ri plasmids, Springer Verlag, 2009年, 01, 単行本(学術書), 共著, ISSN 1862-5576, 978-3-540-85466
  2. 2002年, Endocytobiology and Cell Organelles. Endocytobiology VIII. Proceedings of the Eighth International Colloquium on Endocytobiology and Symbiosis, Nagoya, October 12-17, 2001. M. Sugiura et al. (eds). Logos, Berlin (2002), An overview of the Agrobacterial genome; the Hetero-trimer constitution by tumor inducing plasmid, linear and circular chromosomal DNAs, Logos, Berlin, 2002年, 調査報告書, Suzuki K, Uraji M, Nagata Y, Urbanczyk H, Bautista J, Yoshida K, de Costa D, Hattori Y, Ohta N, Iwata K, Satou M, Sonoda H, Miyahara K, Masuda A
  3. 2002年, Endocytobiology and Cell Organelles. Endocytobiology VIII. Proceedings of the Eighth International Colloquium on Endocytobiology and Symbiosis, Nagoya, October 12-17, 2001. M. Sugiura et al. (eds). Logos, Berlin (2002), Genome analysis of Agrobacterium tumefacien, Logos, Berlin, 2002年, 調査報告書, de Costa D, Suzuki K, Satou M, Yoshida K
  4. 2002年, Endocytobiology and Cell Organelles. Endocytobiology VIII. Proceedings of the Eighth International Colloquium on Endocytobiology and Symbiosis, Nagoya, October 12-17, 2001. M. Sugiura et al. (eds). Logos, Berlin (2002), Genetic overview of the right region of the linear chromosomal DNA by linking analysis of fosmid clones in Agrobacterium tumefaciens MAFF301001, the bacterium unique for the possession of both linear and circular chromosomal DNAs as well as forthe abilit, Logos, Berlin, 2002年, 調査報告書, DM De Costa, 鈴木克周, 佐藤M, 吉田和夫
  5. 2004年, Recent Research Development in Plant & Cell Physiology, Research Signpost, Kerala, India, The first complete sequencing analyses of plant tumor-inducing plasmid Ti and root-inducing plasmid Ri indicate their chimeric structures and unique evolutional relationships, Research Singpost, Kerala, India, 2004年, 単行本(学術書), 共著, K. Yoshida, M. Uraji, Y. Hattori, K. Moriguchi, K. Suzuki, N. Tanaka, A. Kato

外部資金

競争的資金等の採択状況

  1. 科学研究費助成事業(基盤研究(B)), イネの形質転換に適した特質を決定するアグロバクテリア遺伝子の解明, 2015年, 2017年
  2. 科学研究費助成事業(挑戦的研究(萌芽)), グラム陽性菌とアグロバクテリアを連携使用する核酸注入技術, 2017年, 2018年