佐久間 哲史TETSUSHI SAKUMA

Last Updated :2017/08/01

所属・職名
大学院理学研究科 数理分子生命理学専攻 特任講師
ホームページ
メールアドレス
tetsushi-sakumahiroshima-u.ac.jp

基本情報

主な職歴

  • 2013年04月01日, 2015年03月31日, 広島大学, 大学院理学研究科, 特任助教
  • 2012年04月01日, 2013年03月31日, 日本学術振興会, 特別研究員PD

学歴

  • 広島大学, 大学院理学研究科, 数理分子生命理学専攻, 日本, 2012年03月
  • 広島大学, 大学院理学研究科, 生物科学専攻, 日本, 2010年03月
  • 広島大学, 理学部, 生物科学科, 日本, 2008年03月

学位

  • 博士(理学) (広島大学)
  • 修士(理学) (広島大学)

研究分野

  • 総合生物 / ゲノム科学 / システムゲノム科学

研究キーワード

  • ゲノム編集
  • TALEN
  • CRISPR/Cas9
  • 遺伝子ノックアウト
  • 遺伝子ノックイン

所属学会

教育活動

授業担当

  1. 2017年, 学部専門, セメスター(前期), 生物科学基礎実験I
  2. 2017年, 修士課程・博士課程前期, セメスター(後期), ゲノミクス
  3. 2017年, 修士課程・博士課程前期, セメスター(前期), 分子遺伝学セミナー
  4. 2017年, 修士課程・博士課程前期, セメスター(後期), 分子遺伝学セミナー

研究活動

学術論文(★は代表的な論文)

  1. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89巻, pp.180-pp.189, MAY 2016
  2. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12巻, 1号, JAN 2016
  3. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83巻, 1号, pp.61-pp.70, JAN 2016
  4. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20巻, 12号, pp.1059-pp.1076, DEC 2015
  5. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40巻, 2号, pp.79-pp.94, 2015
  6. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10巻, 11号, NOV 18 2015
  7. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16巻, 10号, pp.23849-pp.23866, OCT 2015
  8. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51巻, 9号, pp.879-pp.884, OCT 2015
  9. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158巻, 4号, pp.339-pp.353, OCT 2015
  10. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17巻, 2号, pp.178-pp.194, AUG 6 2015
  11. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5巻, 1号, pp.75-pp.82, JUL 14 2015
  12. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43巻, 13号, pp.6359-pp.6372, JUL 27 2015
  13. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403巻, 1号, pp.43-pp.56, JUL 1 2015
  14. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5巻, JUN 9 2015
  15. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112巻, 7号, pp.1335-pp.1342, JUL 2015
  16. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15巻, MAY 22 2015
  17. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16巻, APR 29 2015
  18. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10巻, 3号, MAR 17 2015
  19. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5巻, MAR 5 2015
  20. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10巻, 5号, pp.664-pp.673, FEB 10 2015
  21. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156巻, 2号, pp.735-pp.744, FEB 2015
  22. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4巻, 1号, pp.143-pp.154, JAN 13 2015
  23. ★, Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5巻, NOV 2014
  24. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4巻, NOV 20 2014
  25. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14巻, NOV 18 2014
  26. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4巻, OCT 1 2014
  27. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26巻, 9号, pp.3763-pp.3774, SEP 2014
  28. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4巻, AUG 27 2014
  29. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206巻, 3号, pp.347-pp.356, AUG 4 2014
  30. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63巻, 3号, pp.349-pp.355, JUL 2014
  31. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4巻, JUL 16 2014
  32. ★, Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4巻, JUN 23 2014
  33. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52巻, 5号, pp.431-pp.439, MAY 2014
  34. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19巻, 5号, pp.419-pp.431, MAY 2014
  35. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111巻, 4号, pp.1461-pp.1466, JAN 28 2014
  36. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141巻, 2号, pp.481-pp.487, JAN 15 2014
  37. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.78-pp.85, JAN 2014
  38. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.108-pp.114, JAN 2014
  39. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.92-pp.97, JAN 2014
  40. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.115-pp.121, JAN 2014
  41. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.86-pp.91, JAN 2014
  42. Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp.2-pp.13, JAN 2014
  43. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63巻, 1号, pp.79-pp.84, JAN 2014
  44. ★, Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3巻, NOV 29 2013
  45. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3巻, NOV 5 2013
  46. ★, Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18巻, 4号, pp.315-pp.326, APR 2013
  47. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
  48. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 2013 Mar
  49. Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 2013 Feb
  50. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377巻, 1号, pp.275-pp.283, MAY 1 2013
  51. Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 2013 Mar
  52. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375巻, 1号, pp.79-pp.91, MAR 1 2013
  53. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
  54. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 2012 Jul
  55. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 2012 Jun
  56. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 2011 Aug
  57. The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 2010 Mar