Nobukazu Tanaka

Last Updated :2022/01/05

Affiliations, Positions
Natural Science Center for Basic Research and Development, Professor
E-mail
ntanahiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 1996/10/01, 2003/03/31, Hiroshima University, Center for Gene Science, Assistant Professor
  • 1994/06/01, 1996/09/30, Hiroshima University, Center for Gene Science, Research Assistant
  • 1987/04/01, 1993/12/31, Daicel Chemical Industries, Research Center, Researcher
  • 2003/04/01, Hiroshima University, Natural Science Center for Basic Research and Development, Assistant Professor
  • 2007/04/01, Hiroshima University, Natural Science Center for Basic Research and Development, Professor

Academic Degrees

  • Doctor of Agriculture, Nagoya University
  • Master of Agriculture, Nagoya University

Educational Activity

  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology

In Charge of Primary Major Programs

  • Biotechnology

Research Fields

  • Biology;Basic biology;Plant molecular biology / Plant physiology
  • Agricultural sciences;Plant production and environmental agriculture;Science in genetics and breeding

Research Keywords

  • Gene
  • Differentiation
  • Root
  • Cell wall

Affiliated Academic Societies

  • The Botanical Sociedty of Japan
  • The Molecular Biology Society of Japan
  • The Jopanese Society of Plant Physiologists
  • Japanese Society for Plant Cell and Molecular Biology

Educational Activity

Course in Charge

  1. 2021, Liberal Arts Education Program1, 1Term, Ethics and regulations in natural science research
  2. 2021, Undergraduate Education, 3Term, Basic life science
  3. 2021, Graduate Education (Master's Program) , 3Term, Life Science and Gene Technology B
  4. 2021, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  5. 2021, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  6. 2021, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  7. 2021, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  8. 2021, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  9. 2021, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  10. 2021, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  11. 2021, Graduate Education (Master's Program) , OutOfTerm(1st), Ethics of Genome Editing Research

Research Activities

Academic Papers

  1. Molecular Cloning and Characterization of L-Galactose-1-phosphate Phosphatase from Tobacco (Nicotiana tabacum), BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 76(6), 1155-1162, 201206
  2. Nucleotide Sequence of the rol Region of Mikimopine-type Root-inducing Plasmid pRi1724, Bioscience= Biotechnology and Biochemistry, 58(3), 548, 19940401
  3. Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library, DNA Research, 7, 157-163, 20000401
  4. Viability of plant hairy roots is sustained without propagation in low sugar medium kept at ambient temperature, Biochemical Engineering Journal, 6, 75-80, 20000401
  5. Genome structure of Ri plasmid (3). Sequencing analysis of the vir region of pRi1724 in Japanese Agrobacterium rhizogenes, Nucleic Acids Symposium Series, 44, 95-96, 20000401
  6. Expression of betaine aldehyde dehydrogenase gene in transgenic tomato hairy roots leads to the accumulation of glycine betaine and contributes to the maintenance of the osmotic potential under salt stress, Soil Science and Plant Nutrition, 46(4), 873-883, 20000401
  7. Genome structure of Ri plasmid (2). Sequencing analysis of T-DNA and its flanking regions of pRi1724 in Japanese Agrobacterium rhizogenes, Nucleic Acids Symposium Series, 42, 67-68, 19990401
  8. Genome structure of Ri plasmid (1). Construction of linking library and physical map of pRi1724 in Japanese Agrobacterium rhizogenes, Nucleic Acids Symposium Series, 39, 189-190, 19980401
  9. Efficient DNA minipreparation by modified benzyl chloride method from hairy roots of Ajuga reptans to detect rolB gene from Ri plasmid, Plant Biotechnology, 15(4), 245-247, 19980401
  10. Agrobacterium rhizogenes-mediated transformation and regeneration of legume Astragalus sinicus (Chinese milk vetch), Plant Science, 138, 53-65, 19980401
  11. Partial contig map of the smallest chromosome in Coprinus cinereus, Mycoscience, 38, 127-133, 19970401
  12. Strategies for the production of secondary metabolites by pRi-transformed regenerants, Plant Tissue Culture and Biotechnology, 3(3), 128-137, 19970401
  13. Efficient regeneration from GUS-transformed Ajuga hairy root, Journal of Fermentation and Bioengineering, 81(5), 374-378, 19960401
  14. Transformation of Egyptian berseem (Trifolium alexandrium L.) by Agrobacterium rhizogenes, Egyptian Journal of Genetics and Cytology, 24, 233-242, 19950401
  15. Antibiotic resistance of Agrobacterium strains isolated in Japan, Annals of the Phytopathological Society of Japan, 61, 444-450, 19950401
  16. Clonal propagation of 20-hydroxyecdysone producing plant, Pfaffia iresinoides, Plant Tissue Culture Letters, 12(2), 187-191, 19950401
  17. Vincamine production in multiple shoot culture derived from hairy roots of Vinca minor, Plant Cell= Tissue and Organ Culture, 41, 61-64, 19950401
  18. Agrobacterium rhizogenes-mediated transformation and regeneration of Vinca minor L., Plant Tissue Culture Letters, 11(3), 191-198, 19940401
  19. 3b-hydroxy-5b-cholest-7-en-6-one as an intermediate of 20-hydroxyecdysone biosynthesis in a hairy root culture of Ajuga reptans var. atropurpurea, Journal of Chemical Society= Chemical Communication, 1761-1762, 19940401
  20. Incorporation of acetate and cholesterol into 20-hydroxyecdysone by hairy root clone of Ajuga reptans var. atropurpurea, Phytochemistry, 36(4), 907-910, 19940401
  21. Restriction endonuclease map of the root-inducing plasmid (pRi1724) of Agrobacterium rhizogenes strain MAFF03-01724, Biosciences Biotechnology and Biochemistry, 58(2), 297-299, 19940401
  22. Identification of rol genes on pRi1724 in Agrobacterium rhizogenes strain MAFF03-01724 isolated in Japan, Annals of the Phytopathological Society of Japan, 60(1), 45-52, 19940401
  23. ★, Regenerants from Ajuga hairy roots with high productivity of 20-hydroxyecdysone, Plant Cell Reports, 13, 87-90, 19930401
  24. Characterization of Ajuga plants regenerated from hiary roots, Plant Tissue Culture Letters, 10(1), 78-83, 19930401
  25. Introduction of DNA into Agrobacterium spp. by electroporation, Plant Tissue Culture Letters, 10(2), 194-196, 19930401
  26. Transfer and analysis of pRi1724 in Agrobacterium rhizogenes strain MAFF03-01724 by conjugation or electroporation, Annals of the Phytopathological Society of Japan, 59(5), 594-600, 19930401
  27. Transformation of Agrobacterium rhizogenes straing MAFF03-01724 by electroporation, Annals of the Phytopathological Society of Japan, 59(5), 587-593, 19930401
  28. Molecular analysis of T-DNA region on the root inducing plasmid (Ri) in a mikimopine type Agrobacterium rhizogenes strain 1724, Annals of the Phytopathological Society of Japan, 59(2), 155-162, 19930401
  29. ★, Production of phytoecdysteroids by hairy root cultures of Ajuga reptans var. atropurpurea, Agricultural and Biological Chemistry, 55(4), 1019-1025, 19910401
  30. Transient expression of lacZ gene in protoplasts of Orychophragmus violaceus, Plant Tissue Culture Letters, 8(2), 73-81, 19910401
  31. Detection of opines by paper electrophoresis, Plant Tissue Culture Letters, 7(1), 45-47, 19900401
  32. Regenerants of horseradish hairy roots incited by Agrobacterium rhizogenes infection, Plant Cell Reports, 6, 283-286, 19870401
  33. Infection of turnip and radish storage roots with Agrobacterium rhizogenes, Plant Cell Reports, 4, 74-77, 19850401
  34. E. coli spheroplast-mediated transfer of cloned cauliflower mosaic virus DNA into plant protoplasts, Molecular General Genetics, 195, 378-380, 19840401
  35. Endocytic uptake of Escherichia coli spheroplasts by Neurospora crassa slime cells - An Ultrastructural Study, European Journal of Applied Microbiology and Biotechnology, 19, 296-299, 19840401
  36. Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts, Plant Cell Reports, 2, 30-32, 19830401
  37. Hairy Root-mediated Transgenic-Plant Regeneration in Egyptian Clover (Trifolium alexandrinum L.), Plant Biotechnology, 21(2), 165-168, 20040401
  38. The first complete sequencing analyses of plant tumor-inducing plasmid and root-inducing plasmid Ri indicate their chimeric structures and unique evlutional relationships, Recent Res. Devel. Plant Cell Physiol., 1, 53-61, 20030401
  39. Evidence of horizontal gene transfer from Agrobacterium rhizogenes to tobacco plants during the evolutionary process, Endocytobiosis and Cell Research, 15, 151-162, 20040401
  40. Genome structure and evolution of giant plant pathogenic plasmids in Agrobacterium tumefaciens and Agrobacterium rhizogenes, Endocytobiosis and Cell Research, 15, 371-378, 20040401
  41. Analysis of Unique Variable Region of a Plant Root Inducing Plasmid, pRi1724, by the Construction of its Physical Map and Library, DNA Research, 7, 157-163, 20000401
  42. Viability of plant hairy roots is sustained without propagation in low sugar medium kept at ambient temperature, BIOCHEMICAL ENGINEERING JOURNAL, 6(1), 75-80, 200008
  43. Analysis of unique variable region of a plant root inducing plasmid, pRi1724, by the construction of its physical map and library, DNA RESEARCH, 7(3), 157-163, 20000630
  44. Expression of betaine aldehyde dehydrogenase gene in transgenic tomato hairy roots leads to the accumulation of glycine betaine and contributes to the maintenance of the osmotic potential under salt stress, SOIL SCIENCE AND PLANT NUTRITION, 46(4), 873-883, 200012
  45. Proliferation and rol gene expression in hairy root lines of Egyptian clover, PLANT CELL TISSUE AND ORGAN CULTURE, 66(3), 175-182, 2001
  46. ★, Mikimopine synthase (mis) gene on pRi1724, GENE, 263(1-2), 49-58, 20010124
  47. The complete nucleotide sequence of a plant root-inducing (Ri) plasmid indicates its chimeric structure and evolutionary relationship between tumor-inducing (Ti) and symbiotic (Sym) plasmids in Rhizobiaceae, JOURNAL OF MOLECULAR BIOLOGY, 307(3), 771-784, 20010330
  48. ★, Tobacco plants were transformed by Agrobacterium rhizogenes infection during their evolution, PLANT JOURNAL, 32(5), 775-787, 200212
  49. ★, Nuclear localization and interaction of RolB with plant 14-3-3 proteins correlates with induction of adventitious roots by the oncogene rolB, PLANT JOURNAL, 38(2), 260-275, 200404
  50. ★, Characterization of the rolB promoter on mikimopine-type pRi1724 T-DNA, PLANT SCIENCE, 168(5), 1353-1364, 200505
  51. Hairy root-activation tagging: A high-throughput system for activation tagging in transformed hairy roots, PLANT MOLECULAR BIOLOGY, 59(5), 793-807, 200511
  52. Characterization of salt tolerance in ectoine-transformed tobacco plants (Nicotiana tabaccum): photosynthesis, osmotic adjustment, and nitrogen partitioning, PLANT CELL AND ENVIRONMENT, 29(2), 173-182, 200602
  53. Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene, PLANT AND CELL PHYSIOLOGY, 49(1), 126-132, 200801
  54. Modification of the surface carbohydrate composition of tobacco protoplasts transformed with the human UDP-galactose transporter gene hUGT1, PLANT BIOTECHNOLOGY, 26(4), 435-441, 20090925
  55. ★, The impact of the overexpression of human UDP-galactose transporter gene hUGT1 in tobacco plants, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109(2), 159-169, 201002
  56. Fission yeast Ubr1 ubiquitin ligase influences the oxidative stress response via degradation of active Pap1 bZIP transcription factor in the nucleus, MOLECULAR MICROBIOLOGY, 80(3), 739-755, 201105
  57. Essential role of Ubr11, but not Ubr1, as an N-end rule ubiquitin ligase in Schizosaccharomyces pombe, YEAST, 30(1), 1-11, 201301
  58. Functional characterization of a new grapevine MYB transcription factor and regulation of proanthocyanidin biosynthesis in grapes, JOURNAL OF EXPERIMENTAL BOTANY, 65(15), 4433-4449, 201408
  59. UDP-galactose transporter gene hUGT1 expression in tobacco plants leads to hyper-galactosylated cell wall components, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(5), 573-583, 201605
  60. Analysis of GDP-D-mannose pyrophosphorylase gene promoter from acerola (Malpighia glabra) and increase in ascorbate content of transgenic tobacco expressing the acerola gene, PLANT AND CELL PHYSIOLOGY, 49(1), 126-132, 2008
  61. Meta-analysis of transcriptomic responses to biotic and abiotic stress in tomato, PEERJ, 6, 20180717
  62. Expression of the human UDP-galactose transporter gene hUGT1 in tobacco plants' enhanced plant hardness, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 126(2), 241-248, 201808
  63. Agrobacterium-mediated transformation of ovary of bread wheat (Triticum aestivum L.) with a gene encoding a tomato ERF protein., Plant Cell Biotech. Mol. Biol., 19, 24-33, 2018
  64. Expression of a fungal exo-beta-1,3-galactanase in Arabidopsis reveals a role of type II arabinogalactans in the regulation of cell shape, JOURNAL OF EXPERIMENTAL BOTANY, 71(18), 5414-5424, 20200919
  65. Effects of vacuum infiltration, Agrobacterium cell density and acetosyringone concentration on Agrobacterium-mediated transformation of bread wheat, JOURNAL OF CONSUMER PROTECTION AND FOOD SAFETY, 16(1), 59-69, 202103

Publications such as books

  1. 1993, Transformation in horseradish (Armoracia rusticana): Hairy roots incited by Agrobacterium rhizogenes infection. , Biotechnology in Agriculture and Forestry=Vol.23= Protoplasts and Genetic Engineering IV= Springer-Verlag, 1993, Scholarly Book, Joint work
  2. 1999, Genetic Transformation of Ajuga reptans(共著) , Biotechnology in Agriculture and Forestry= Vol.45= Transgenic Medicinal Plants= Springer-Verlag, 1999, Scholarly Book, Joint work
  3. 1999, Genetic Transformation of Vinca minor L. , Biotechnology in Agriculture and Forestry= Vol.45= Transgenic Medicinal Plants= Springer-Verlag, 1999
  4. 1999/02, New current of biotechnology , 1999, 02, Textbook, Cocompilation, 4254171064, 156
  5. 2008/02, Agrobacterium: From biology to biotechnology, Springer, 2008, 02, Scholarly Book, Cocompilation, 9780387722894, 750

Invited Lecture, Oral Presentation, Poster Presentation

  1. CRISPR/Cas9-mediated disruption and analysis of tobacco Rox1 (rolB overexpressed 1) gene, Kenta Tsuchiyama. Yuka Obara, Yunyo Liang, Tsuyoshi Nakagawa, Nobukazu Tanaka, The 38rd Annual Meeting The Botanical Society of Japan, 2019/09/15, Without Invitation, Japanese, The Botanical Society of Japan, Sendai (Tohoku University)
  2. CRISPR/Cas9-mediated disruption and analysis of tobacco Rox1 (rolB overexpressed 1) gene, Kenta Tsuchiyama, Yuka Obara, Tsuyoshi Nakagawa, Nobukazu Tanaka, 4th Annual Meeting of The Japanese Society for Genome Editing, 2019/06/05, Without Invitation, Japanese, The Japanese Society for Genome Editing, Tokyo
  3. Functional analysis of Agrobacterium-derived Acs gene in Ipomoea batatas., Tanaka, A., Takemoto, D., Suzuki, T., Uesaka, K., Yamaguchi, N., Otani, M., Nakayachi, O., Arakawa K. and Tanaka, N., 2020 Annual Meeting of the Phytopathological Society of Japan (PSJ) Kansai Division, 2020/11/07, Without Invitation, Japanese, the Phytopathological Society of Japan (PSJ) Kansai Division, Shimane University (On-line)
  4. Safety management of genetic modification experiments undertaken by AAPGS in Japan, Nobukazu Tanaka, The 43rd Annual Meeting of the Molecular Biology Society of Japan, 2020/12/02, With Invitation, Japanese, The Molecular Biology Society of Japan, On-line
  5. A new approach toward the artificial manipulation of polysaccharide of arabinogalactan protein, Tanaka N, Kotake T, 2015/03/29, With Invitation, Japanese
  6. Genome structure and evolution of giant plant pathogenic plasmids in Agrobacterium tumefaciens and Agrobacterium rhizogenes., Kazuo Yoshida, Misugi Uraji, Yoshiyuki Hattori, Kazuki Moriguchi, Katsunori Suzuki, Nobukazu Tanaka, Akira Kato, International Congress on Endocytobiology and Symbiosis, 2001, Without Invitation, English, Nagoya, Japan
  7. Adventitious root induction by the rooting locus gene B (rolB) correlates with the nuclear localization and interaction of RolB protein with plant 14-3-3 proteins., Hiroshi Moriuchi, Ichiro Yamashita, Yasunori Machida, Nobukazu Tanaka, 25th Crown Gall Conference, 2004, Without Invitation, English, Illinois, USA
  8. Effects of overexpressed human UDP-galactose transporter 1 gene (hUGT1) on hUGT1-transgenic tobacco plants., Nobukazu Tanaka, Mohammed F. M. Khalil, Takahiro Horibe, Tetsuya Kawahara, Kan-ichi Suzuki, Nobuhiro Ishida, 20th IUBMB International Congress of Biochemistry and Molecular Biology and 11th FAOBMB Congress, 2006, Without Invitation, English, Kyoto, Japan
  9. Characteristics of cultured tobacco BY-2 cells transformed with the rooting locus B (rolB) gene of Agrobacterium rhizogenes., Nobukazu Tanaka, Saki Sakamoto, Hidemi Tsuru, Minori Nishihara, International Symposium on Adventitious Root Formation, 2008, Without Invitation, English, Madrid, Spain
  10. Characteristics of cultured tobacco BY-2 cells transformed with the rooting locus B (rolB) gene of Agrobacterium rhizogenes., Nobukazu Tanaka, Saki Sakamoto, Hidemi Tsuru, Minori Nishihara, International Symposium on Adventitious Root Formation, 2008, Without Invitation, English, Madrid, Spain
  11. Hyper-galactosylation of plant cell wall components leads to alteration of plant growth and characteristics., Nobukazu Tanaka, 5th International Symposium on Plant Signaling & Behavior 2017, 2017/06, With Invitation, English, The Society of Plant Signaling and Behavior, Matsue, Japan
  12. Response of tobacco plants overexpressing UDP-Gal epimerase to Gal toxicity., Tayebeh Abedi, Nobukazu Tanaka, 5th International Symposium on Plant Signaling & Behavior 2017, Without Invitation, English, The Society of Plant Signaling and Behavior, Matsue, Japan