TETSUSHI SAKUMA

Last Updated :2022/11/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
Web Site
E-mail
tetsushi-sakumahiroshima-u.ac.jp
Self-introduction
I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with my great collaborators.

Basic Information

Major Professional Backgrounds

  • 2012/04/01, 2013/03/31, Japan Society for the Promotion of Science (JSPS), JSPS Research Fellow (PD)
  • 2013/04/01, 2015/03/31, Hiroshima University, Graduate School of Science, Assistant Professor (Special Appointment)
  • 2015/04/01, 2018/03/31, Hiroshima University, Graduate School of Science, Lecturer (Special Appointment)
  • 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Science, Lecturer
  • 2019/04/01, 2020/03/31, Hiroshima University, Graduate School of Integrated Sciences for Life, Lecturer
  • 2020/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Associate Professor

Educational Backgrounds

  • Osaka University, Japan, 2002/04, 2006/03
  • Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2006/04, 2008/03
  • Hiroshima University, Graduate School of Science, Department of Biological Science, Japan, 2008/04, 2010/03
  • Hiroshima University, Graduate School of Science, Department of Mathematical and Life Sciences, Japan, 2010/04, 2012/03

Academic Degrees

  • Doctor of Science (Ph.D.), Hiroshima University
  • Master of Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Science : Biological Sciences : Biology
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science

Research Fields

  • Biological Sciences;Genome science;System genome science
  • Biological Sciences;Genome science;Medical genome science

Research Keywords

  • Genome editing
  • Epigenome editing
  • TALEN
  • CRISPR-Cas9
  • Gene knockout
  • Gene knockin

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2022, Undergraduate Education, 2Term, Advanced Biology
  2. 2022, Undergraduate Education, 1Term, Basic Biological Science A
  3. 2022, Undergraduate Education, 3Term, Developmental Biology A
  4. 2022, Undergraduate Education, 2Term, Genome Biology
  5. 2022, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
  6. 2022, Undergraduate Education, First Semester, Special Study for Graduation
  7. 2022, Undergraduate Education, Second Semester, Special Study for Graduation
  8. 2022, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  9. 2022, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  10. 2022, Graduate Education (Master's Program) , 2Term, societal implementation of life science
  11. 2022, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
  12. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
  13. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Life Science A
  14. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Life Science A
  15. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Life Science B
  16. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Life Science B
  17. 2022, Graduate Education (Master's Program) , 2Term, Molecular Genetics
  18. 2022, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science C
  19. 2022, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science D
  20. 2022, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  21. 2022, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
  22. 2022, Graduate Education (Master's Program) , 3Term, Introduction to Disease Models
  23. 2022, Graduate Education (Master's Program) , 1Term, Exercises in Biomedical Science A
  24. 2022, Graduate Education (Master's Program) , 2Term, Exercises in Biomedical Science A
  25. 2022, Graduate Education (Master's Program) , 3Term, Exercises in Biomedical Science B
  26. 2022, Graduate Education (Master's Program) , 4Term, Exercises in Biomedical Science B
  27. 2022, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  28. 2022, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  29. 2022, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  30. 2022, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
  31. 2022, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E
  32. 2022, Graduate Education (Master's Program) , 2Term, Basics and Practical Applications of Genome Editing
  33. 2022, Graduate Education (Master's Program) , OutOfTerm(1st), Basic Training of Genome Editing
  34. 2022, Graduate Education (Doctoral Program) , 3Term, Trends of Genome Editing in Life Science

Research Activities

Academic Papers

  1. Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology, SCIENTIFIC REPORTS, 12(1), 20220427
  2. Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga, SCIENTIFIC REPORTS, 12(1), 20220215
  3. Panel of human cell lines with human/mouse artificial chromosomes, SCIENTIFIC REPORTS, 12(1), 20220222
  4. Gene manipulation in the Mucorales fungus Rhizopus oryzae using TALENs with exonuclease overexpression, FEMS MICROBIOLOGY LETTERS, 369(1), 20220222
  5. TALEN-mediated generation of Nkx3.1 knockout rat model, PROSTATE, 81(3), 182-193, 202102
  6. Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation, NATURE METABOLISM, 3(2), 196-+, 202102
  7. CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin, DEVELOPMENTAL BIOLOGY, 472, 85-97, 202104
  8. Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 131(4), 373-380, 202104
  9. Pou5f3.3 is involved in establishment and maintenance of hematopoietic cells during Xenopus development, TISSUE & CELL, 72, 202110
  10. TALEN-Mediated Gene Editing of slc24a5 (Solute Carrier Family 24, Member 5) in Kawakawa, Euthynnus affinis, JOURNAL OF MARINE SCIENCE AND ENGINEERING, 9(12), 202112
  11. Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus, EXPERIMENTAL ANIMALS, 69(1), 26-33, 202001
  12. Hox13 is essential for formation of a sensory organ at the terminal end of the sperm duct in Ciona, DEVELOPMENTAL BIOLOGY, 458(1), 120-131, 20200201
  13. EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD, ELIFE, 9, 20200217
  14. GABA-Induced GnRH Release Triggers Chordate Metamorphosis, CURRENT BIOLOGY, 30(8), 1555-+, 20200420
  15. Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 56(5), 359-366, 202005
  16. Reinvestigation of Disulfide-bonded Oligomeric Forms of the Unfolded Protein Response Transducer ATF6, CELL STRUCTURE AND FUNCTION, 45(1), 9-21, 2020
  17. ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk, TRANSLATIONAL PSYCHIATRY, 10(1), 20200722
  18. Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis, SCIENTIFIC REPORTS, 10(1), 20200213
  19. Six1is required for signaling center formation and labial-lingual asymmetry in developing lower incisors, DEVELOPMENTAL DYNAMICS, 249(9), 1098-1116, 202009
  20. DJ-1 is indispensable for the S-nitrosylation of Parkin, which maintains function of mitochondria, SCIENTIFIC REPORTS, 10(1), 20200309
  21. Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga,Nannochloropsis oceanicaNIES-2145, GENES TO CELLS, 25(10), 695-702, 202010
  22. Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells, BRITISH JOURNAL OF CANCER, 122(6), 823-834, 20200317
  23. Regenerating islet-derived protein (Reg)3 beta plays a crucial role in attenuation of ileitis and colitis in mice, BIOCHEMISTRY AND BIOPHYSICS REPORTS, 21, 202003
  24. Nucleotide receptor P2RY4 is required for head formation via induction and maintenance of head organizer in Xenopus laevis, DEVELOPMENT GROWTH & DIFFERENTIATION, 61(2), 186-197, 201902
  25. Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116(8), 3072-3081, 20190219
  26. Loss of HCN1 subunits causes absence epilepsy in rats, BRAIN RESEARCH, 1706, 209-217, 20190301
  27. PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching, PLOS ONE, 14(3), 20190306
  28. KLF1 mutation E325K induces cell cycle arrest in erythroid cells differentiated from congenital dyserythropoietic anemia patient-specific induced pluripotent stem cells, EXPERIMENTAL HEMATOLOGY, 73, 25-37, 201905
  29. Anephrogenic phenotype induced by SALL1 gene knockout in pigs, SCIENTIFIC REPORTS, 9, 20190529
  30. Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors, STEM CELL REPORTS, 13(2), 322-337, 20190813
  31. Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system, DEVELOPMENT GROWTH & DIFFERENTIATION, 61(6), 378-388, 201908
  32. Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis), SCIENTIFIC REPORTS, 9, 20190925
  33. Efficient genome engineering using Platinum TALEN in potato, PLANT BIOTECHNOLOGY, 36(3), 167-173, 201909
  34. TAp63 represses transcription of MYCN/NCYM gene and its high levels of expression are associated with favorable outcome in neuroblastoma, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 518(2), 311-318, 20191015
  35. Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference, PLOS ONE, 13(1), 20180129
  36. Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, 201802
  37. Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, 20180216
  38. Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, 20180305
  39. Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, 201804
  40. Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, 201805
  41. Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, 201808
  42. Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, 201807
  43. ★, Biased genome editing using the local accumulation of DSB repair molecules system, NATURE COMMUNICATIONS, 9, 20180816
  44. Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes, STEM CELL REPORTS, 11(3), 727-740, 20180911
  45. Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 505(2), 453-459, 20181028
  46. MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(10), 201810
  47. Acceleration of cancer science with genome editing and related technologies, CANCER SCIENCE, 109(12), 3679-3685, 201812
  48. Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays, CRISPR JOURNAL, 1(5), 337-347, 201810
  49. Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation, EXPERIMENTAL ANIMALS, 67(4), 535-543, 201812
  50. PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer, CELL DEATH DISCOVERY, 4, 20180208
  51. Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, 201703
  52. Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, 20170315
  53. Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, 20170515
  54. Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, 20170519
  55. Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, 20170501
  56. Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
  57. Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
  58. PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, 20170703
  59. ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), 20171117
  60. TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
  61. Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, 201601
  62. Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), 201601
  63. Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, 201605
  64. Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, 20160412
  65. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, 201604
  66. Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, 201605
  67. Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, 20160510
  68. Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, 201605
  69. Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, 201606
  70. Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, 201606
  71. Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, 201607
  72. Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, 20160707
  73. Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, 20160815
  74. Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, 201611
  75. Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, 20161128
  76. Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, 20161124
  77. Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, 201612
  78. Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), 20161228
  79. C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, 20161220
  80. ★, MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11(1), 118-133, 201601
  81. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
  82. Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, 20150113
  83. Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, 20150305
  84. A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), 20150317
  85. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, 201502
  86. Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, 20150429
  87. Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, 20150522
  88. Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, 201507
  89. Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, 20150609
  90. Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, 20150701
  91. Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, 20150727
  92. Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, 20150714
  93. Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, 20150806
  94. Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, 201510
  95. Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, 201510
  96. Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, 201510
  97. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), 20151118
  98. Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
  99. Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, 201512
  100. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, 201401
  101. TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
  102. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, 201401
  103. Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, 201401
  104. Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 2-13, 201401
  105. Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, 201401
  106. TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, 201401
  107. Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, 201401
  108. FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, 201405
  109. Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, 201405
  110. ★, Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, 20140623
  111. Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, 20140716
  112. Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, 20140827
  113. Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, 201407
  114. EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, 20140804
  115. Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, 20141001
  116. ★, Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, 201411
  117. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 20141120
  118. Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, 201409
  119. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, 20141118
  120. Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, 20140115
  121. Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, 20130301
  122. ★, Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
  123. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, 20130501
  124. Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, 20131105
  125. ★, Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, 20131129
  126. Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
  127. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 201303
  128. Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 201302
  129. Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 201303
  130. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
  131. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 201207
  132. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 201206
  133. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 201108
  134. The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 201003

Invited Lecture, Oral Presentation, Poster Presentation

  1. Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Seki F, Yurimoto T, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, International Conference on Alzheimer's and Parkinson's Diseases (AD/PD 2022), 2022/03/14, Without Invitation, English
  2. Recent advances in genome editing and beyond, Sakuma T, International Symposium on Nanomedicine 2021 (ISNM2021), 2021/11/17, With Invitation, English
  3. Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Seki F, Yurimoto T, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, New Horizons in Alzheimer's Disease, 2021/10/27, Without Invitation, English
  4. Sequence-based parameters contributing to the efficiency of MMEJ-assisted knock-in and Prime Editing, Nakamae K, Yamamoto K, Takenaga M, Nakade S, Tagashira N, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2021/08/18, Without Invitation, English
  5. Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, Alzheimer's Association International Conference 2021 (AAIC 2021), 2021/07/26, Without Invitation, English
  6. Transgene-free and highly efficient genome editing using removable all-in-one Platinum TALEN-ARS plasmids in microalga, Nannochloropsis, Kurita T, Iwai M, Okazaki K, Nomura S, Saito F, Takami A, Sakamoto A, Ohta H, Sakuma T, Yamamoto T, International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB), 2021/06/14, Without Invitation, English
  7. Optimization of dCas9-based transcription activator systems using a collection of variously patterned RNA aptamers and protein tags, Kunii A, Nakamura S, Yamamoto T, Sakuma T, Keystone Symposia - Precision Engineering of the Genome, Epigenome and Transcriptome, 2021, Without Invitation, English
  8. Versatile collections of transcriptional control toolkits with highly accumulable effectors using Class 1 and Class 2 CRISPR systems, Nakamura S, Kunii A, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, Keystone Symposia - Engineering the Genome, 2020, Without Invitation, English
  9. MaChIAto enables detailed profiling of genomic and epigenomic features affecting the efficacy of MMEJ-assisted knock-in, Nakamae K, Takenaga M, Nakade S, Mitsuhashi T, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Keystone Symposia - Engineering the Genome, 2020, Without Invitation, English
  10. Development of transcriptional activation platforms of cancer-related genes using Class 1 and Class 2 CRISPR systems, Tetsushi Sakuma, 2020, With Invitation, English
  11. MaChIAto: detailed profiling tool for various genomic features affecting the efficacy of gene knockout, homology-based knock-in, and Prime Editing, Sakuma T, Nakamae K, Takenaga M, Nakade S, Mitsuhashi T, Nazuka I, Awazu A, Sakamoto N, Yamamoto T, CRISPR and Beyond: Perturbations at Scale to Understand Genomes, 2020, Without Invitation, English
  12. Comprehensive analysis of highly variable transcriptional activation platforms based on Class 1 and Class 2 CRISPR systems, Nakamura S, Kunii A, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, The International CRISPR and Gene Editing Symposium, 2020, Without Invitation, English
  13. Extended analysis of amplicon sequencing data with MaChIAto for prime editing, Nakamae K, Takenaga M, Nakade S, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2020, Without Invitation, English
  14. Highly organized, variously patterned accumulation platforms of transcription activators using Class 1 and Class 2 CRISPR systems, Kunii A, Nakamura S, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2020, Without Invitation, English
  15. Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Sasaguri H, Kumita W, Nagata K, Sakuma T, Yamamoto T, Saido TC, Sasaki E, Alzheimer's Association International Conference (AAIC) 2020, 2020, Without Invitation, English
  16. Genesis: Marmoset model of familial Alzheimer's disease, Saido TC, Sato K, Sasaguri H, Kumita W, Nagata K, Sakuma T, Yamamoto T, Sasaki E, AAT-AD/PD™ 2020 Conference: Advances in Alzheimer's and Parkinson's Therapies, 2020, Without Invitation, English

Awards

  1. 2021, Distinguished Researcher, Hiroshima University
  2. 2018, Distinguished Researcher, Hiroshima University
  3. 2017, Phoenix Outstanding Researcher Award, Hiroshima University
  4. 2016, Phoenix Outstanding Researcher Award, Hiroshima University
  5. 2015, President Award, Hiroshima University

Patented

  1. Patent, 2014258386, 2019/02/07
  2. Patent, 11201508730T, 2018/04/16
  3. Patent, 10189879, 2019/01/29
  4. Patent, 10030235, 2018/07/24
  5. Patent, 10006011, 2018/06/26
  6. Patent, 6487306, 2019/03/01
  7. Patent, 6806822, 2020/12/08
  8. Patent, ZL201480059441.7, 2021/02/02
  9. Patent, BR112015026707-6, 2020/12/29
  10. Patent, 2018271425, 2021/03/11

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2021, 2023
  2. KAKENHI(Grant-in-Aid for Early-Career Scientists), 2019, 2020
  3. Research Program on Hepatitis , 2018/04/01, 2019/03/31
  4. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  5. Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
  6. Research Program on Hepatitis , 2017/04/01, 2018/03/31
  7. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  8. Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
  9. Research Program on Hepatitis , 2016/04/01, 2017/03/31
  10. Design and functional evaluation of artificial transcriptional activator, 2016/04/01, 2017/03/31
  11. KAKENHI, 2016, 2017
  12. KAKENHI, 2015, 2018
  13. KAKENHI, 2013, 2014
  14. KAKENHI, 2012, 2013