TETSUSHI SAKUMA
Last Updated :2023/11/02
- Affiliations, Positions
- Graduate School of Integrated Sciences for Life, Professor
- Web Site
- E-mail
- tetsushi-sakuma
hiroshima-u.ac.jp
- Self-introduction
- I am investigating the genome editing systems, methods, and techniques, and applying them in various cells and organisms with wonderful collaborators.
Basic Information
Major Professional Backgrounds
- 2012/04/01, 2013/03/31, Japan Society for the Promotion of Science (JSPS), JSPS Research Fellow (PD)
- 2013/04/01, 2015/03/31, Hiroshima University, Graduate School of Science, Assistant Professor (Special Appointment)
- 2015/04/01, 2018/03/31, Hiroshima University, Graduate School of Science, Lecturer (Special Appointment)
- 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Science, Lecturer
- 2019/04/01, 2020/03/31, Hiroshima University, Graduate School of Integrated Sciences for Life, Lecturer
- 2020/04/01, 2023/03/31, Hiroshima University, Graduate School of Integrated Sciences for Life, Associate Professor
- 2023/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Professor
Educational Backgrounds
- Osaka University, Japan, 2002/04, 2006/03
- Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2006/04, 2008/03
- Hiroshima University, Graduate School of Science, Department of Biological Science, Japan, 2008/04, 2010/03
- Hiroshima University, Graduate School of Science, Department of Mathematical and Life Sciences, Japan, 2010/04, 2012/03
Academic Degrees
- Doctor of Science (Ph.D.), Hiroshima University
- Master of Science, Hiroshima University
Educational Activity
- [Bachelor Degree Program] School of Science : Biological Sciences : Biology
- [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
- [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
- [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
- [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
Research Fields
- Biological Sciences;Genome science;System genome science
- Biological Sciences;Genome science;Medical genome science
Research Keywords
- Genome editing
- Epigenome editing
- TALEN
- CRISPR-Cas9
- Gene knockout
- Gene knockin
Affiliated Academic Societies
- The Japanese Society for Genome Editing
- The Molecular Biology Society of Japan
- Japanese Society of Developmental Biologists
- Japanese Cancer Association
Educational Activity
Course in Charge
- 2023, Liberal Arts Education Program1, 1Term, Fundamentals of Biology
- 2023, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
- 2023, Undergraduate Education, 2Term, Advanced Biology
- 2023, Undergraduate Education, 1Term, Basic Biological Science A
- 2023, Undergraduate Education, 3Term, Developmental Biology A
- 2023, Undergraduate Education, 2Term, Genome Biology
- 2023, Undergraduate Education, Second Semester, Seminar for Gene Chemistry
- 2023, Undergraduate Education, First Semester, Special Study for Graduation
- 2023, Undergraduate Education, Second Semester, Special Study for Graduation
- 2023, Undergraduate Education, First Semester, Practice for Fundamental Biology I
- 2023, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
- 2023, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
- 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Mathematical and Life Sciences
- 2023, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
- 2023, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
- 2023, Graduate Education (Master's Program) , First Semester, Exercises in Life Science A
- 2023, Graduate Education (Master's Program) , Second Semester, Exercises in Life Science B
- 2023, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
- 2023, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
- 2023, Graduate Education (Master's Program) , 3Term, Introduction to Disease Models
- 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
- 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
- 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E
- 2023, Graduate Education (Master's Program) , OutOfTerm(1st), Basic Training of Genome Editing
- 2023, Graduate Education (Doctoral Program) , 3Term, Trends of Genome Editing in Life Science
Research Activities
Academic Papers
- Transcription activator-like effector nuclease-mediated deletion safely eliminates the major egg allergen ovomucoid in chickens, FOOD AND CHEMICAL TOXICOLOGY, 175, 202305
- Developmental changes in brain activity of heterozygous Scn1a knockout rats, FRONTIERS IN NEUROLOGY, 14, 20230314
- Pigs with an INS point mutation derived from zygotes electroporated with CRISPR/ Cas9 and ssODN, FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 11, 20230113
- Establishment of CRFK cells for vaccine production by inactivating endogenous retrovirus with TALEN technology, SCIENTIFIC REPORTS, 12(1), 20220427
- Genome editing with removable TALEN vectors harboring a yeast centromere and autonomous replication sequence in oleaginous microalga, SCIENTIFIC REPORTS, 12(1), 20220215
- Panel of human cell lines with human/mouse artificial chromosomes, SCIENTIFIC REPORTS, 12(1), 20220222
- Gene manipulation in the Mucorales fungus Rhizopus oryzae using TALENs with exonuclease overexpression, FEMS MICROBIOLOGY LETTERS, 369(1), 20220222
- TALEN-mediated generation of Nkx3.1 knockout rat model, PROSTATE, 81(3), 182-193, 202102
- Murine neonatal ketogenesis preserves mitochondrial energetics by preventing protein hyperacetylation, NATURE METABOLISM, 3(2), 196-+, 202102
- CRISPR-Cas9 editing of non-coding genomic loci as a means of controlling gene expression in the sea urchin, DEVELOPMENTAL BIOLOGY, 472, 85-97, 202104
- Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 131(4), 373-380, 202104
- Pou5f3.3 is involved in establishment and maintenance of hematopoietic cells during Xenopus development, TISSUE & CELL, 72, 202110
- TALEN-Mediated Gene Editing of slc24a5 (Solute Carrier Family 24, Member 5) in Kawakawa, Euthynnus affinis, JOURNAL OF MARINE SCIENCE AND ENGINEERING, 9(12), 202112
- Pathological characteristics of Ccdc85c knockout rats: a rat model of genetic hydrocephalus, EXPERIMENTAL ANIMALS, 69(1), 26-33, 202001
- Hox13 is essential for formation of a sensory organ at the terminal end of the sperm duct in Ciona, DEVELOPMENTAL BIOLOGY, 458(1), 120-131, 20200201
- EDEM2 stably disulfide-bonded to TXNDC11 catalyzes the first mannose trimming step in mammalian glycoprotein ERAD, ELIFE, 9, 20200217
- GABA-Induced GnRH Release Triggers Chordate Metamorphosis, CURRENT BIOLOGY, 30(8), 1555-+, 20200420
- Various strategies of effector accumulation to improve the efficiency of genome editing and derivative methodologies, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 56(5), 359-366, 202005
- Reinvestigation of Disulfide-bonded Oligomeric Forms of the Unfolded Protein Response Transducer ATF6, CELL STRUCTURE AND FUNCTION, 45(1), 9-21, 2020
- ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk, TRANSLATIONAL PSYCHIATRY, 10(1), 20200722
- Three multi-allelic gene pairs are responsible for self-sterility in the ascidian Ciona intestinalis, SCIENTIFIC REPORTS, 10(1), 20200213
- Six1is required for signaling center formation and labial-lingual asymmetry in developing lower incisors, DEVELOPMENTAL DYNAMICS, 249(9), 1098-1116, 202009
- DJ-1 is indispensable for the S-nitrosylation of Parkin, which maintains function of mitochondria, SCIENTIFIC REPORTS, 10(1), 20200309
- Efficient and multiplexable genome editing using Platinum TALENs in oleaginous microalga,Nannochloropsis oceanicaNIES-2145, GENES TO CELLS, 25(10), 695-702, 202010
- Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells, BRITISH JOURNAL OF CANCER, 122(6), 823-834, 20200317
- Regenerating islet-derived protein (Reg)3 beta plays a crucial role in attenuation of ileitis and colitis in mice, BIOCHEMISTRY AND BIOPHYSICS REPORTS, 21, 202003
- Nucleotide receptor P2RY4 is required for head formation via induction and maintenance of head organizer in Xenopus laevis, DEVELOPMENT GROWTH & DIFFERENTIATION, 61(2), 186-197, 201902
- Humanized UGT2 and CYP3A transchromosomic rats for improved prediction of human drug metabolism, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 116(8), 3072-3081, 20190219
- Loss of HCN1 subunits causes absence epilepsy in rats, BRAIN RESEARCH, 1706, 209-217, 20190301
- PDIP38/PolDIP2 controls the DNA damage tolerance pathways by increasing the relative usage of translesion DNA synthesis over template switching, PLOS ONE, 14(3), 20190306
- KLF1 mutation E325K induces cell cycle arrest in erythroid cells differentiated from congenital dyserythropoietic anemia patient-specific induced pluripotent stem cells, EXPERIMENTAL HEMATOLOGY, 73, 25-37, 201905
- Anephrogenic phenotype induced by SALL1 gene knockout in pigs, SCIENTIFIC REPORTS, 9, 20190529
- Activin Is Superior to BMP7 for Efficient Maintenance of Human iPSC-Derived Nephron Progenitors, STEM CELL REPORTS, 13(2), 322-337, 20190813
- Establishment of knockout adult sea urchins by using a CRISPR-Cas9 system, DEVELOPMENT GROWTH & DIFFERENTIATION, 61(6), 378-388, 201908
- Targeted mutagenesis of the ryanodine receptor by Platinum TALENs causes slow swimming behaviour in Pacific bluefin tuna (Thunnus orientalis), SCIENTIFIC REPORTS, 9, 20190925
- Efficient genome engineering using Platinum TALEN in potato, PLANT BIOTECHNOLOGY, 36(3), 167-173, 201909
- TAp63 represses transcription of MYCN/NCYM gene and its high levels of expression are associated with favorable outcome in neuroblastoma, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 518(2), 311-318, 20191015
- Tailor-made gene silencing of Staphylococcus aureus clinical isolates by CRISPR interference, PLOS ONE, 13(1), 20180129
- Cancer induction and suppression with transcriptional control and epigenome editing technologies, JOURNAL OF HUMAN GENETICS, 63(2), 187-194, 201802
- Scleraxis is a transcriptional activator that regulates the expression of Tenomodulin, a marker of mature tenocytes and ligamentocytes, SCIENTIFIC REPORTS, 8, 20180216
- Microhomology-assisted scarless genome editing in human iPSCs, NATURE COMMUNICATIONS, 9, 20180305
- Unexpected heterogeneity derived from Cas9 ribonucleoprotein-introduced clonal cells at the HPRT1 locus, GENES TO CELLS, 23(4), 255-263, 201804
- Targeted knock-in of an scFv-Fc antibody gene into the hprt locus of Chinese hamster ovary cells using CRISPR/Cas9 and CRIS-PITCh systems, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 599-605, 201805
- Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering, BIOMATERIALS, 173, 11-21, 201808
- Differential micronucleus frequency in isogenic human cells deficient in DNA repair pathways is a valuable indicator for evaluating genotoxic agents and their genotoxic mechanisms, ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 59(6), 529-538, 201807
- ★, Biased genome editing using the local accumulation of DSB repair molecules system, NATURE COMMUNICATIONS, 9, 20180816
- Organoids from Nephrotic Disease-Derived iPSCs Identify Impaired NEPHRIN Localization and Slit Diaphragm Formation in Kidney Podocytes, STEM CELL REPORTS, 11(3), 727-740, 20180911
- Generation of and characterization of anti-IL-11 antibodies using newly established Il11-deficient mice, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 505(2), 453-459, 20181028
- MET Activation by a Macrocyclic Peptide Agonist that Couples to Biological Responses Differently from HGF in a Context-Dependent Manner, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 19(10), 201810
- Acceleration of cancer science with genome editing and related technologies, CANCER SCIENCE, 109(12), 3679-3685, 201812
- Three-Component Repurposed Technology for Enhanced Expression: Highly Accumulable Transcriptional Activators via Branched Tag Arrays, CRISPR JOURNAL, 1(5), 337-347, 201810
- Electroporation-mediated genome editing in vitrified/warmed mouse zygotes created by IVF via ultra-superovulation, EXPERIMENTAL ANIMALS, 67(4), 535-543, 201812
- PLEKHN1 promotes apoptosis by enhancing Bax-Bak hetro-oligomerization through interaction with Bid in human colon cancer, CELL DEATH DISCOVERY, 4, 20180208
- Detailed analysis of targeted gene mutations caused by the Platinum-Fungal TALENs in Aspergillus oryzae RIB40 strain and a ligD disruptant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 287-293, 201703
- Germ cell regeneration-mediated, enhanced mutagenesis in the ascidian Ciona intestinalis reveals flexible germ cell formation from different somatic cells, DEVELOPMENTAL BIOLOGY, 423(2), 111-125, 20170315
- Culture time of vitrified/warmed zygotes before microinjection affects the production efficiency of CRISPR-Cas9-mediated knock-in mice, BIOLOGY OPEN, 6(5), 706-713, 20170515
- Highly efficient biallelic genome editing of human ES/iPS cells using a CRISPR/Cas9 or TALEN system, NUCLEIC ACIDS RESEARCH, 45(9), 5198-5207, 20170519
- Hox-mediated endodermal identity patterns pharyngeal muscle formation in the chordate pharynx, DEVELOPMENT, 144(9), 1629-1634, 20170501
- Cas9, Cpf1 and C2c1/2/3-What's next?, BIOENGINEERED, 8(3), 265-273, 2017
- Establishment of expanded and streamlined pipeline of PITCh knock-in - a web-based design tool for MMEJ-mediated gene knock-in, PITCh designer, and the variations of PITCh, PITCh-TG and PITCh-KIKO, BIOENGINEERED, 8(3), 302-308, 2017
- PAX2 is dispensable for in vitro nephron formation from human induced pluripotent stem cells, SCIENTIFIC REPORTS, 7, 20170703
- ALC1/CHD1L, a chromatin-remodeling enzyme, is required for efficient base excision repair, PLOS ONE, 12(11), 20171117
- TALEN-mediated targeted editing of the GDE5 gene suppresses fibroblastic cell proliferation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 81(11), 2164-2167, 2017
- Efficient modification of the myostatin gene in porcine somatic cells and generation of knockout piglets, MOLECULAR REPRODUCTION AND DEVELOPMENT, 83(1), 61-70, 201601
- Functional Investigation of a Non-coding Variant Associated with Adolescent Idiopathic Scoliosis in Zebrafish: Elevated Expression of the Ladybird Homeobox Gene Causes Body Axis Deformation, PLOS GENETICS, 12(1), 201601
- Depdc5 knockout rat: A novel model of mTORopathy, NEUROBIOLOGY OF DISEASE, 89, 180-189, 201605
- Establishment of In Vitro FUS-Associated Familial Amyotrophic Lateral Sclerosis Model Using Human Induced Pluripotent Stem Cells, STEM CELL REPORTS, 6(4), 496-510, 20160412
- In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21(4), 358-369, 201604
- Temporal effects of Notch signaling and potential cooperation with multiple downstream effectors on adenohypophysis cell specification in zebrafish, GENES TO CELLS, 21(5), 492-504, 201605
- Systematic Cellular Disease Models Reveal Synergistic Interaction of Trisomy 21 and GATA1 Mutations in Hematopoietic Abnormalities, CELL REPORTS, 15(6), 1228-1241, 20160510
- Single-Cell-State Culture of Human Pluripotent Stem Cells Increases Transfection Efficiency, BIORESEARCH OPEN ACCESS, 5(1), 127-136, 201605
- Human Induced Pluripotent Stem Cell-Derived Podocytes Mature into Vascularized Glomeruli upon Experimental Transplantation, JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY, 27(6), 1778-1791, 201606
- Establishment of Functional Genomics Pipeline in Epiblast-Like Tissue by Combining Transcriptomic Analysis and Gene Knockdown/Knockin/Knockout, Using RNA Interference and CRISPR/Cas9, HUMAN GENE THERAPY, 27(6), 436-450, 201606
- Involvement of aspartoacylase in tremor expression in rats, EXPERIMENTAL ANIMALS, 65(3), 293-301, 201607
- Generation of a Nonhuman Primate Model of Severe Combined Immunodeficiency Using Highly Efficient Genome Editing, CELL STEM CELL, 19(1), 127-138, 20160707
- Ultra-superovulation for the CRISPR-Cas9-mediated production of gene-knockout, single-amino-acid-substituted, and floxed mice, BIOLOGY OPEN, 5(8), 1142-1148, 20160815
- Highly multiplexed CRISPR-Cas9-nuclease and Cas9-nickase vectors for inactivation of hepatitis B virus, GENES TO CELLS, 21(11), 1253-1262, 201611
- Gene cassette knock-in in mammalian cells and zygotes by enhanced MMEJ, BMC GENOMICS, 17, 20161128
- Non-RVD mutations that enhance the dynamics of the TAL repeat array along the superhelical axis improve TALEN genome editing efficacy, SCIENTIFIC REPORTS, 6, 20161124
- Functional consequence of fibulin-4 missense mutations associated with vascular and skeletal abnormalities and cutis laxa, MATRIX BIOLOGY, 56, 132-149, 201612
- Transcriptional regulation of a horizontally transferred gene from bacterium to chordate, PROCEEDINGS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES, 283(1845), 20161228
- C-Type Lectin Receptor DCAR Recognizes Mycobacterial Phosphatidyl-Inositol Mannosides to Promote a Th1 Response during Infection, IMMUNITY, 45(6), 1245-1257, 20161220
- ★, MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11(1), 118-133, 201601
- The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
- Precise Correction of the Dystrophin Gene in Duchenne Muscular Dystrophy Patient Induced Pluripotent Stem Cells by TALEN and CRISPR-Cas9, STEM CELL REPORTS, 4(1), 143-154, 20150113
- Precise in-frame integration of exogenous DNA mediated by CRISPR/Cas9 system in zebrafish, SCIENTIFIC REPORTS, 5, 20150305
- A High Excision Potential of TALENs for Integrated DNA of HIV-Based Lentiviral Vector, PLOS ONE, 10(3), 20150317
- Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156(2), 735-744, 201502
- Cloning-free CRISPR/Cas system facilitates functional cassette knock-in in mice, GENOME BIOLOGY, 16, 20150429
- Production of knockout mice by DNA microinjection of various CRISPR/Cas9 vectors into freeze-thawed fertilized oocytes, BMC BIOTECHNOLOGY, 15, 20150522
- Tailor-made TALEN system for highly efficient targeted gene replacement in the rice blast fungus, BIOTECHNOLOGY AND BIOENGINEERING, 112(7), 1335-1342, 201507
- Generation of mutant mice via the CRISPR/Cas9 system using FokI-dCas9, SCIENTIFIC REPORTS, 5, 20150609
- Hox10-regulated endodermal cell migration is essential for development of the ascidian intestine, DEVELOPMENTAL BIOLOGY, 403(1), 43-56, 20150701
- Smarcal1 promotes double-strand-break repair by nonhomologous end-joining, NUCLEIC ACIDS RESEARCH, 43(13), 6359-6372, 20150727
- Genome Editing in Mouse Spermatogonial Stem Cell Lines Using TALEN and Double-Nicking CRISPR/Cas9, STEM CELL REPORTS, 5(1), 75-82, 20150714
- Robust In Vitro Induction of Human Germ Cell Fate from Pluripotent Stem Cells, CELL STEM CELL, 17(2), 178-194, 20150806
- Desmocollin-2 alone forms functional desmosomal plaques, with the plaque formation requiring the juxtamembrane region and plakophilins, JOURNAL OF BIOCHEMISTRY, 158(4), 339-353, 201510
- Homeolog-specific targeted mutagenesis in Xenopus laevis using TALENs, IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL, 51(9), 879-884, 201510
- Homologous Recombination-Independent Large Gene Cassette Knock-in in CHO Cells Using TALEN and MMEJ-Directed Donor Plasmids, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 16(10), 23849-23866, 201510
- The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10(11), 20151118
- Epithelial DLD-1 Cells with Disrupted E-cadherin Gene Retain the Ability to Form Cell Junctions and Apico-basal Polarity, CELL STRUCTURE AND FUNCTION, 40(2), 79-94, 2015
- Relative contribution of four nucleases, CtIP, Dna2, Exo1 and Mre11, to the initial step of DNA double-strand break repair by homologous recombination in both the chicken DT40 and human TK6 cell lines, GENES TO CELLS, 20(12), 1059-1076, 201512
- Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63(1), 79-84, 201401
- TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
- Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 108-114, 201401
- Transcription activator-like effector nucleases efficiently disrupt the target gene in Iberian ribbed newts (Pleurodeles waltl), an experimental model animal for regeneration, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 115-121, 201401
- Nuclease-mediated genome editing: At the front-line of functional genomics technology, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 2-13, 201401
- Versatile strategy for isolating transcription activator-like effector nuclease-mediated knockout mutants in Caenorhabditis elegans, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 78-85, 201401
- TALEN-induced gene knock out in Drosophila, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 86-91, 201401
- Targeted mutagenesis in sea urchin embryos using TALENs, DEVELOPMENT GROWTH & DIFFERENTIATION, 56(1), 92-97, 201401
- FAST-id system for enrichment of cells with TALEN-induced mutations and large deletions, GENES TO CELLS, 19(5), 419-431, 201405
- Germ Cell Mutations of the Ascidian Ciona intestinalis With TALE Nucleases, GENESIS, 52(5), 431-439, 201405
- ★, Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system, SCIENTIFIC REPORTS, 4, 20140623
- Highly efficient targeted mutagenesis in one-cell mouse embryos mediated by the TALEN and CRISPR/Cas systems, SCIENTIFIC REPORTS, 4, 20140716
- Down syndrome-associated haematopoiesis abnormalities created by chromosome transfer and genome editing technologies, SCIENTIFIC REPORTS, 4, 20140827
- Application of Oocyte Cryopreservation Technology in TALEN-Mediated Mouse Genome Editing, EXPERIMENTAL ANIMALS, 63(3), 349-355, 201407
- EDEM2 initiates mammalian glycoprotein ERAD by catalyzing the first mannose trimming step, JOURNAL OF CELL BIOLOGY, 206(3), 347-356, 20140804
- Simple knockout by electroporation of engineered endonucleases into intact rat embryos, SCIENTIFIC REPORTS, 4, 20141001
- ★, Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5, 201411
- Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 20141120
- Sterol Side Chain Reductase 2 Is a Key Enzyme in the Biosynthesis of Cholesterol, the Common Precursor of Toxic Steroidal Glycoalkaloids in Potato, PLANT CELL, 26(9), 3763-3774, 201409
- Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex, BMC BIOTECHNOLOGY, 14, 20141118
- Tissue-specific and ubiquitous gene knockouts by TALEN electroporation provide new approaches to investigating gene function in Ciona, DEVELOPMENT, 141(2), 481-487, 20140115
- Enhancer activity sensitive to the orientation of the gene it regulates in the chordate genome, DEVELOPMENTAL BIOLOGY, 375(1), 79-91, 20130301
- ★, Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
- The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, DEVELOPMENTAL BIOLOGY, 377(1), 275-283, 20130501
- Production of Sry knockout mouse using TALEN via oocyte injection, SCIENTIFIC REPORTS, 3, 20131105
- ★, Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, SCIENTIFIC REPORTS, 3, 20131129
- Efficient gene targeting by TAL effector nucleases coinjected with exonucleases in zygotes, Sci Rep, 2013
- High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, Biol Open, 201303
- Quantitative assay for TALEN activity at endogenous genomic loci, Biol Open, 201302
- Efficient targeted mutagenesis in medaka using custom-designed transcription activator-like effector nucleases, Genetics, 201303
- Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, Nat Commun, 2012
- Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, Proc Natl Acad Sci USA, 201207
- Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, Dev Growth Differ, 201206
- HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development., Dev Genes Evol, 201108
- The third type III module of human fibronectin mediates cell adhesion and migration, J Biochem, 201003
Publications such as books
- 2023, Genome Editing in Animals, Methods in Molecular Biololgy, Updated Overview of TALEN Construction Systems, Springer Nature, 2023, Joint work
- 2023, Epigenomics, Methods in Molecular Biology, Design, Construction, and Validation of Targeted Gene Activation with TREE System in Human Cells, Springer Nature, 2023, Atsushi Kunii, Takashi Yamamoto, Tetsushi Sakuma
- 2022, Scientific Reports, Genome editing, Springer Nature, 2022, Joint work, Maura McGrail, Tetsushi Sakuma, Leonidas Bleris
- 2022, Gene and Genome Editing, From nuclease-based gene knock-in to prime editing - promising technologies of precision gene engineering., Elsevier, 2022, Single work, Tetsushi Sakuma
Invited Lecture, Oral Presentation, Poster Presentation
- Inhibition of OCT4 binding at the MYCN locus induces neuroblastoma cell death accompanied by alteration of protein-coding potential of RNAs, Nakatani K, Kogashi H, Sakuma T, Yamamoto T, Hippo Y, Suenaga Y, Advances in Neuroblastoma Research (ANR) meeting 2023, 2023/05, English, Amsterdam, the Netherlands
- Generation of non-human primate models of Alzheimer's disease, Hiroki Sasaguri, Kenya Sato, Wakako Kumita, Takashi Inoue, Yoko Kurotaki, Fumiko Seki, Terumi Yurimoto, Kenichi Nagata, Naomi Mihira, Kaori Sato, Tetsushi Sakuma, Takashi Yamamoto, Michihira Tagami, Riichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Erika Sasaki, and Takaomi C. Saido, AD/PD 2023 Alzheimer's & Parkinson's Diseases Conference, 2023/03, English, Gothenburg, Sweden
- Targeted TCR gene editing using Platinum TALEN and long single-stranded DNA donor template, Kayo Toishigawa, Kenta Magoori, Taro Edahiro, Hiroyuki Sato, Saori Fukunaga, Yasuko Honjo, Hiroshi Ureshino, Tetsushi Sakuma, Takashi Yamamoto, Ryuji Suzuki, Tatsuo Ichinohe, 2023/02
- Pitch Designer 2.0: Fully Automated Sequence Design Tool for CRISPR-Cas9-Mediated Knock-in Using Template for Short Homology-Based DSB Repair, Kazuki Nakamae, Shota Nakade, Naoaki Sakamoto, Tetsushi Sakuma and Takashi Yamamoto, Plant and Animal Genome Conference / PAG 30, 2023/01, English, San Diego, CA, USA
- From nuclease-based genome editing to DNA double-strand break-free precision gene engineering, Tetsushi Sakuma, International Symposium on Nanomedicine 2022 (ISNM2022), 2022/12, With Invitation, English, Tokushima, Japan
- Developmental Changes in Brain Activity in Scn1a knockout rats: Analysis Using Manganese-Enhanced Magnetic Resonance Imaging, Mayu Tahara, Norimichi Higurashi, Junichi Hata, Masako Nishikawa, Ken Ito, Shinichi Hirose, Takehito Kaneko, Tomoji Mashimo, Tetsushi Sakuma, Takashi Yamamoto, Hirotaka J Okano, The American Epilepsy Society 2022 Annual Meeting, 2022/12, English, Nashville, Tennessee, USA
- Functional relationship between phosphatidylcholine profiles and skeletal muscle function - Metabolic analyses of skeletal muscle-specific GDE5 deficient mouse, Rahmawati Aisyah, Noriyasu Ohshima, Daiki Watanabe, Tetsushi Sakuma, Yoshiko Nakagawa, Thanutchaporn Kumrungsee, Shinji Miura, Takashi Yamamoto, Masanobu Wada, Noriyuki Yanaka, 22nd IUNS-International Congress of Nutrition, 2022/12, English, Tokyo
- Development of MMEJ-based long-range gene replacement with highly multiplexed CRISPR-Cas9, Shu Matsuzaki, Tetsushi Sakuma, and Takashi Yamamoto, 2022/11
- Exploratory research for epigenetic regulatory elements involved in genome editing efficiency using plant nucleosome reconstruction, Tetsushi Sakuma, Minoru Ueda, 2022/11, Japanese
- CRISPR-dCas9-based inhibition of OCT4 binding at the MYCN locus induces neuroblastoma cell death, 2022/11
- Effect of Platinum TALEN mRNA nucleic acid modification on T cell receptor gene editing efficiency, Kayo Toishigawa, Taro Edahiro, Kenta Magoori, Hiroyuki Sato, Yasuko Honjo, Masatoshi Nishizawa, Tetsushi Sakuma, Takashi Yamamoto, Ryuji Suzuki, Tatsuo Ichinohe, 2022/10, Japanese
- Development of concurrent MMEJ-assisted fusional knock-in technique to insert a long gene cassette in human cells and mouse embryos mediated by CRISPR-Cas9, Mio Kawai, Koji Takahashi, Mitsumasa Takenaga, Yukiko U. Inoue, Shota Nakade, Naoaki Sakamoto, Tetsushi Sakuma and Takashi Yamamoto, Cold Spring Harbor Laboratory meeting - Genome Engineering: CRISPR Frontiers, 2022/08, Without Invitation, English, Cold Spring Harbor, NY, USA
- Highly specific and efficient C-to-T and A-to-G base editing with TALE-deaminases assisted by Type I or Type II CRISPR systems, Tetsushi Sakuma, Nahoko Nishibori, Tadahiko Yoshima, Takashi Yamamoto, Cold Spring Harbor Laboratory meeting - Genome Engineering: CRISPR Frontiers, 2022/08, Without Invitation, English, Cold Spring Harbor, NY, USA
- Sequence-based parameters contributing to the efficiency and accuracy of MMEJ-assisted knock-in and Prime Editing, Kazuki Nakamae, Kunihisa Yamamoto, Mitsumasa Takenaga, Shota Nakade, Naomi Tagashira, Ichiro Nazuka, Akinori Awazu, Naoaki Sakamoto, Tetsushi Sakuma and Takashi Yamamoto, FASEB Science Research Coference - The Genome Engineering Conference: Cutting-edge Research and Applications, 2022/06, Without Invitation, English, Lisbon, Portugal
- HIGHLY SPECIFIC AND EFFICIENT C-TO-T AND A-TO-G BASE EDITING BY CAS9 AND TALE COOPERATION, Tetsushi Sakuma, Nahoko Nishibori, Tadahiko Yoshima, Takashi Yamamoto, FASEB Science Research Coference - The Genome Engineering Conference: Cutting-edge Research and Applications, 2022/06, Without Invitation, English, Lisbon, Portugal
- Current place of cutting-edge genome editing, Tetsushi Sakuma, 2022/06, With Invitation, Japanese
- Analysis of Brain Activity in Rats with Dravet Syndrome Using Manganese-Enhanced Magnetic Resonance Imaging, Mayu Tahara, Norimichi Higurashi, Junichi Hata, Masako Nishikawa, Ken Ito, Shinichi Hirose, Takehito Kaneko, Tomoji Mashimo, Tetsushi Sakuma, Takashi Yamamoto, Hirotaka J Okano, 2022/06, Without Invitation
- Generation of non-human primate models of Alzheimer's disease, Hiroki Sasaguri, Kenya Sato, Wakako Kumita, Takashi Inoue, Yoko Kurotaki, Fumiko Seki, Terumi Yurimoto, Kenichi Nagata, Naomi Mihira, Kaori Sato, Tetsushi Sakuma, Takashi Yamamoto, Michihira Tagami, Riichiroh Manabe, Kokoro Ozaki, Yasushi Okazaki, Erika Sasaki, and Takaomi C. Saido, 2022/05, Without Invitation
- Quantitative analysis of chromosomal translocations using digital PCR in gene-edited primary T cells, Kayo Toishigawa, Taichi Hashiba, Kenta Magoori, Taro Edahiro,
Hiroyuki Sato, Yasuko Honjo, Masatoshi Nishizawa, Tetsushi Sakuma, Takashi Yamamoto, Ryuji Suzuki, Tatsuo Ichinohe, 44th JSTCT Annual Meeting (JSTCT2022), 2022/05, Without Invitation, Japanese
- Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Seki F, Yurimoto T, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, International Conference on Alzheimer's and Parkinson's Diseases (AD/PD 2022), 2022/03/14, Without Invitation, English
- Recent advances in genome editing and beyond, Sakuma T, International Symposium on Nanomedicine 2021 (ISNM2021), 2021/11/17, With Invitation, English
- Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Seki F, Yurimoto T, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, New Horizons in Alzheimer's Disease, 2021/10/27, Without Invitation, English
- Sequence-based parameters contributing to the efficiency of MMEJ-assisted knock-in and Prime Editing, Nakamae K, Yamamoto K, Takenaga M, Nakade S, Tagashira N, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2021/08/18, Without Invitation, English
- Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Kumita W, Inoue T, Kurotaki Y, Nagata K, Mihira N, Sato K, Sakuma T, Yamamoto T, Tagami M, Manabe R, Ozaki K, Okazaki Y, Sasaki E, Saido TC, Alzheimer's Association International Conference 2021 (AAIC 2021), 2021/07/26, Without Invitation, English
- Transgene-free and highly efficient genome editing using removable all-in-one Platinum TALEN-ARS plasmids in microalga, Nannochloropsis, Kurita T, Iwai M, Okazaki K, Nomura S, Saito F, Takami A, Sakamoto A, Ohta H, Sakuma T, Yamamoto T, International Conference on Algal Biomass, Biofuels and Bioproducts (AlgalBBB), 2021/06/14, Without Invitation, English
- Improvement of lipid productivity of Aurantiochytrium sp. by genome editing, Watanabe K, Perez CMT, Kitahori T, Hata K, Aoi M, Takahashi H, Sakuma T, Okamura Y, Nakashimada Y, Yamamoto T, Matsuyama K, Mayuzumi S, Aki T, 2021 AOCS Annual Meeting & Expo, 2021/05/03, Without Invitation, English
- Optimization of dCas9-based transcription activator systems using a collection of variously patterned RNA aptamers and protein tags, Kunii A, Nakamura S, Yamamoto T, Sakuma T, Keystone Symposia - Precision Engineering of the Genome, Epigenome and Transcriptome, 2021, Without Invitation, English
- Versatile collections of transcriptional control toolkits with highly accumulable effectors using Class 1 and Class 2 CRISPR systems, Nakamura S, Kunii A, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, Keystone Symposia - Engineering the Genome, 2020, Without Invitation, English
- MaChIAto enables detailed profiling of genomic and epigenomic features affecting the efficacy of MMEJ-assisted knock-in, Nakamae K, Takenaga M, Nakade S, Mitsuhashi T, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Keystone Symposia - Engineering the Genome, 2020, Without Invitation, English
- Development of transcriptional activation platforms of cancer-related genes using Class 1 and Class 2 CRISPR systems, Tetsushi Sakuma, 2020, With Invitation, English
- MaChIAto: detailed profiling tool for various genomic features affecting the efficacy of gene knockout, homology-based knock-in, and Prime Editing, Sakuma T, Nakamae K, Takenaga M, Nakade S, Mitsuhashi T, Nazuka I, Awazu A, Sakamoto N, Yamamoto T, CRISPR and Beyond: Perturbations at Scale to Understand Genomes, 2020, Without Invitation, English
- Comprehensive analysis of highly variable transcriptional activation platforms based on Class 1 and Class 2 CRISPR systems, Nakamura S, Kunii A, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, The International CRISPR and Gene Editing Symposium, 2020, Without Invitation, English
- Extended analysis of amplicon sequencing data with MaChIAto for prime editing, Nakamae K, Takenaga M, Nakade S, Nazuka I, Awazu A, Sakamoto N, Sakuma T, Yamamoto T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2020, Without Invitation, English
- Highly organized, variously patterned accumulation platforms of transcription activators using Class 1 and Class 2 CRISPR systems, Kunii A, Nakamura S, Yoshimi K, Mashimo T, Yamamoto T, Sakuma T, Cold Spring Harbor Laboratory Meeting - Genome Engineering: CRISPR Frontiers, 2020, Without Invitation, English
- Generation of non-human primate models of Alzheimer's disease, Sasaguri H, Sato K, Sasaguri H, Kumita W, Nagata K, Sakuma T, Yamamoto T, Saido TC, Sasaki E, Alzheimer's Association International Conference (AAIC) 2020, 2020, Without Invitation, English
- Genesis: Marmoset model of familial Alzheimer's disease, Saido TC, Sato K, Sasaguri H, Kumita W, Nagata K, Sakuma T, Yamamoto T, Sasaki E, AAT-AD/PD™ 2020 Conference: Advances in Alzheimer's and Parkinson's Therapies, 2020, Without Invitation, English
Awards
- 2022, Rising Star of Science Award, Research.com
- 2021, Distinguished Researcher, Hiroshima University
- 2018, Distinguished Researcher, Hiroshima University
- 2017, Phoenix Outstanding Researcher Award, Hiroshima University
- 2016, Phoenix Outstanding Researcher Award, Hiroshima University
- 2015, President Award, Hiroshima University
Patented
- Patent, 2014258386, 2019/02/07
- Patent, 11201508730T, 2018/04/16
- Patent, 10189879, 2019/01/29
- Patent, 10030235, 2018/07/24
- Patent, 10006011, 2018/06/26
- Patent, 6487306, 2019/03/01
- Patent, 6806822, 2020/12/08
- Patent, ZL201480059441.7, 2021/02/02
- Patent, BR112015026707-6, 2020/12/29
- Patent, 2018271425, 2021/03/11
External Funds
Acceptance Results of Competitive Funds
- KAKENHI(Grant-in-Aid for Scientific Research (C)), 2021, 2023
- KAKENHI(Grant-in-Aid for Early-Career Scientists), 2019, 2020
- Research Program on Hepatitis , 2018/04/01, 2019/03/31
- Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
- Practical Research for Innovative Cancer Control , 2017/04/01, 2018/03/31
- Research Program on Hepatitis , 2017/04/01, 2018/03/31
- Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
- Practical Research for Innovative Cancer Control , 2016/11/28, 2017/03/31
- Research Program on Hepatitis , 2016/04/01, 2017/03/31
- Design and functional evaluation of artificial transcriptional activator, 2016/04/01, 2017/03/31
- KAKENHI, 2016, 2017
- KAKENHI, 2015, 2018
- KAKENHI, 2013, 2014
- KAKENHI, 2012, 2013