HIROSHI OCHIAI

Last Updated :2021/07/02

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
Web Site
E-mail
ochiaihiroshima-u.ac.jp
Other Contact Details
Kagamiyama 3-10-23,Higashi-Hiroshima 739-0046 JAPAN, Japan
TEL : (+81)82-424-4008 FAX : (+81)82-424-4008
Self-introduction
Gene expression is mainly regulated by transcription, but its regulatory mechanisms are complex and vary widely among genes. Currently, we are conducting research to elucidate the regulatory mechanism of transcription, especially the phenomenon called the transcriptional bursting, using mouse embryonic stem cells.

Basic Information

Major Professional Backgrounds

  • 2021/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Associate Professor
  • 2019/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Associate Professor or Lecturer
  • 2015/10/01, 2019/03/31, JST, Graduate School of Science, Department of Mathematical and Life Sciences, Hiroshima University, PRESTO researcher
  • 2013/04/01, Hiroshima University, Research Center for the Mathematics on Chromatin Live Dynamics (RcMcD), Lecturer (Special Appointment)
  • 2012/06/01, Hiroshima University, Research Institute for Radiation Biology and Medicine, Assistant professor
  • 2011/04/01, 2012/05/31, JSPS, Hiroshima University, Research Fellow PD
  • 2009/04/01, 2011/03/31, JSPS, Hiroshima University, Research Fellow DC2

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Natural Science, Department of Mathematical and Life Sciences, Japan, 2008/04, 2011/03
  • Hiroshima University, Graduate School, Division of Natural Science, Department of Mathematical and Life Sciences, Japan, 2007/04, 2008/03
  • Hiroshima University, Faculty of Science, Departmet of Biological Science, Japan, 2003/04, 2007/03

Academic Degrees

  • Ph.D. (Science), Hiroshima University
  • Master (Science), Hiroshima University

Educational Activity

  • 【Bachelor Degree Program】School of Science : Biological Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science

Research Fields

  • Biological Sciences;Genome science;System genome science
  • Environmental science;Environmental analyses and evaluation;Risk sciences of radiation and chemicals
  • Biological Sciences;Genome science;Medical genome science

Research Keywords

  • single cell analysis
  • pluripotent stem cells
  • ES cell
  • CRISPR
  • Live imaging
  • Gene expression noise
  • zinc-finger nuclease
  • TALEN
  • SNP

Affiliated Academic Societies

  • THE MOLECULAR BIOLOGY SOCIETY OF JAPAN
  • The Japanese Society for Epigenetics
  • The Japanese society for Genome Editing
  • THE BIOPHYSICAL SOCIETY OF JAPAN

Educational Activity

Course in Charge

  1. 2021, Undergraduate Education, 1Term, Systems Biology
  2. 2021, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
  3. 2021, Undergraduate Education, Second Semester, Practice for Fundamental Biology II
  4. 2021, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  5. 2021, Undergraduate Education, 3Term, Laboratory Work in Biology A
  6. 2021, Graduate Education (Master's Program) , 1Term, Exercises in Biomedical Science A
  7. 2021, Graduate Education (Master's Program) , 2Term, Exercises in Biomedical Science A
  8. 2021, Graduate Education (Master's Program) , 1Term, Introduction to Life Science
  9. 2021, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  10. 2021, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
  11. 2021, Graduate Education (Master's Program) , 1Term, Advanced Technologies for Life Science
  12. 2021, Graduate Education (Master's Program) , 3Term, Exercises in Biomedical Science B
  13. 2021, Graduate Education (Master's Program) , 4Term, Exercises in Biomedical Science B
  14. 2021, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  15. 2021, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  16. 2021, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
  17. 2021, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E
  18. 2021, Graduate Education (Doctoral Program) , 3Term, Trends of Genome Editing in Life Science

Research Activities

Academic Papers

  1. ★, Single-gene imaging links genome topology, promoter–enhancer communication and transcription control, Nature Structural & Molecular Biology, 27, 1032-1040, 20200921
  2. ★, Genome-wide kinetic properties of transcriptional bursting in mouse embryonic stem cells, Science Advances, 6(25), eaaz6699, 20200617
  3. Insufficiency of ciliary cholesterol in hereditary Zellweger syndrome, The EMBO Journal, 39, e103499, 20200505
  4. PHi-C: deciphering Hi-C data into polymer dynamics, NAR Genomics and Bioinformatics, 2(2), lqaa020, 20200331
  5. ★, Role of dynamic nuclear deformation on genomic architecture reorganization, PLoS Computational Biology, 15(9), e1007289, 20190911
  6. ★, Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells, Cell, 178(2), 491-506, 20190530
  7. Functional analysis using CRISPR library, 676, 201902
  8. Dynamic changes in the interchromosomal interaction of early histone gene loci during early development of sea urchin, Journal of Cell Science, 130(24), 4097-4107, 201711
  9. Ligation-mediated PCR with a back-to-back adapter reduces amplification bias resulting from variations in GC content, Analytical Biochemistry, 531(15), 37-44, 201705
  10. Analysis of individual differences in radiosensitivity using genome editing, ANNALS OF THE ICRP, 201603
  11. Single-Base Pair Genome Editing in Human Cells by Using Site-Specific Endonucleases, International Journal of Molecular Sciences, 16(9), 21128-21137, 201509
  12. ★, Simultaneous live imaging of the transcription and nuclear position of specific genes, Nucleic Acids Research, 43(19), e127, 20150619
  13. The Microtubule-Depolymerizing Activity of a Mitotic Kinesin Protein KIF2A Drives Primary Cilia Disassembly Coupled with Cell Proliferation, CELL REPORTS, 10(5), 664-673, 20150210
  14. Identification of mutation causing cancer-prone hereditary disease using a novel method for the introduction of single-nucleotide substitution, 252(2), 153-158, 201501
  15. ★, Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, SCIENTIFIC REPORTS, 4, 7125, 20141120
  16. ★, TALEN-mediated single-base-pair editing identification of an intergenic mutation upstream of BUB1B as causative of PCS ( MVA) syndrome, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 111(4), 1461-1466, 20140128
  17. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity., Scientific Reports, 3, 3379, 20131101
  18. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, BIOLOGY OPEN, 2(5), 448-452, 20130515
  19. ★, Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18(4), 315-326, 201304
  20. Genome editing in cultured cells using TALEN, 31(1), 95-100, 201301
  21. Equarin is involved as an FGF signaling modulator in chick lens differentiation, DEVELOPMENTAL BIOLOGY, 368(1), 109-117, 20120801
  22. Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases, NATURE COMMUNICATIONS, 3, 1017, 201208
  23. ★, Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109(27), 10915-10920, 20120703
  24. Targeted disruption of exogenous EGFP gene in medaka using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 546-556, 201206
  25. Efficient targeted mutagenesis of the chordate Ciona intestinalis genome with zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(5), 535-545, 20120528
  26. HpSumf1 is involved in the activation of sulfatases responsible for regulation of skeletogenesis during sea urchin development, DEVELOPMENT GENES AND EVOLUTION, 221(3), 157-166, 201108
  27. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15(8), 875-885, 201008
  28. Dicer is required for the normal development of sea urchin, Hemicentrotus pulcherrimus, ZOOLOGICAL SCIENCE, 27(6), 477-486, 201006
  29. Role of the nanos homolog during sea urchin development, Developmental Dynamics, 238(10), 2511-2521, 200910
  30. Suppressor of Hairless (Su(H)) is required for foregut development in the sea urchin embryo., ZOOLOGICAL SCIENCE, 26(10), 686-690, 200910
  31. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237(9), 2475-2482, 200809
  32. Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus, MECHANISMS OF DEVELOPMENT, 125(1-2), 2-17, 200802

Publications such as books

  1. 2019/08/13, Imaging Gene Expression, Shav-Tal Y. (eds), Real-Time Observation of Localization and Expression (ROLEX) System for Live Imaging of the Transcriptional Activity and Nuclear Position of a Specific Endogenous Gene, Humana, 2019/08/13, 20190813, Scholarly Book, Joint work, Hiroshi Ochiai, 978-1-4939-9674-2, 35-45
  2. 2018/06, 医療応用をめざすゲノム編集 最新動向から技術・倫理的課題まで 真下 知士/金田 安史 編, KAGAKUDOJIN, Single work, Japanese, Hiroshi Ochiai, 274, 65-75
  3. 2017/06, Genome Editing in Animals, Springer New York, Scholarly Book, English, Hiroshi Ochiai, Takashi Yamamoto, 978-1-4939-7128-2, 1-24, Construction and Evaluation of Zinc Finger Nucleases
  4. 2014/12, Targeted Genome Editing Using Site-specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System, Genome Editing Using Zinc-Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs), Springer, 2014, 12, Scholarly Book, English, Hiroshi Ochiai, Takashi Yamamoto, 978-4-431-55227-7, 205, 3-24, Genome editing using zinc-finger nucleases (ZFNs) and transcription activator-like effector nucleases (TALENs)

Invited Lecture, Oral Presentation, Poster Presentation

  1. Establishment of primitive endoderm differentiation reporter mouse ES cell line using CRISPR-Cas9, Hitoshi Owada, Hiroshi Ochiai, Takashi Yamamoto, The 5th Annunal Meeting of JSGE, 2021/06/17, Without Invitation, Japanese, Online
  2. Elucidation of the mechanism of transcriptional burst regulation in mammalian cells, Hiroshi Ochiai, Center for Novel Model Organism Development Open Seminar: Genome Editing Technical Seminar III, 2021/02/03, With Invitation, Japanese, Online
  3. Regulation of transcriptional bursting by chromatin potential, Hiroshi Ochiai, The 43rd Annual Meeting of the Molecular Biology Society of Japan, 2020/12/02, With Invitation, English, Online
  4. Transcriptional bursting induces gene expression heterogeneity in mES cells, Hiroshi Ochiai, Chromosome Dynamics 2019, 2019/12/08, With Invitation, English, Basel
  5. Transcriptional bursting induces gene expression heterogeneity in mouse embryonic stem cells, Hiroshi Ochiai, Single Molecule & Chromatin Symposium, 2019/11/28, With Invitation, English, Melbourne
  6. CRISPR library screening enables identification of transcriptional bursting related genes, Hiroshi Ochiai, Takashi Yamamoto, Takashi Yamamoto, 4th Annual Meeting of The Japanease Society for Genome Editing, 2019/06/05, Without Invitation, English, The Japanease Society for Genome Editing, Tokyo
  7. Role of dynamic nuclear deformation on genomic architecture reorganization, Hiroshi Ochiai, S. Seirin-Lee, Fumitaka Osakada, Junichi Takeda, The 41th Annual Meeting of the Molecular Biology Society of Japan, 2018/11/30, Without Invitation, Japanese
  8. Development of comprehensive analysis technology for intercellular phenotypic heterogeneity, Hiroshi Ochiai, The 41th Annual Meeting of the Molecular Biology Society of Japan, 2018/11/28, Without Invitation, Japanese
  9. Comprehensive analysis of regulatory mechanisms of gene expression noise in mouse embryonic stem cells, Hiroshi Ochiai, The 91st Annual Meeting of the Japanese Biochemical Society, 2018/09/26, Without Invitation, Japanese
  10. Understanding Higher-order genomic structure using genome editing technology, Hiroshi Ochiai, JAAM2018, 2018/05/25, Without Invitation, Japanese
  11. Comprehensive analysis of regulatory mechanisms of intrinsic gene expression noise in mouse embryonic stem cells, Hiroshi Ochiai, Mana Umeda, Tetsutaro Hayashi, Mika Yoshimura, Akihito Harada, Yasuyuki Ohkawa, Takashi Yamamoto, Itoshi Nikaido, The 12th Annual Meeting of the Japanese Society for Epigenetics, 2018/05/24, Without Invitation, Japanese
  12. Relationship between kinetics of higher genome structure and transcription activity, Hiroshi Ochiai, ConBio2017, 2017/12/07, Without Invitation, Japanese, Kobe, Japan
  13. Relationship between kinetics of higher-order genomic structure and transcriptional activity, Hiroshi Ochiai, The 55th Annual Meeting of the Biophysical Society of Japan, 2017/09/21, Without Invitation, English, Kumamoto
  14. Dynamic nuclear deformation induces nuclear architecture remodeling, Hiroshi Ochiai, Seirin S. Lee, The 39th Annual Meeting of the Molecular Biology Society of Japan, 2016/11/30, Without Invitation, English, Yokohama, Japan
  15. Single Base Pair Genome-Editing Technology and Its Future Applications, Hiroshi Ochiai, 13th Japanese-German Frontiers of Science Symposium, 2016/10/07, Without Invitation, English
  16. A challenging interdisciplinary approach to elucidate a mystery of remodeling process in nuclear architecture (Part II: Experiment), Hiroshi Ochiai, JSMB2016, 2016/09/09, Without Invitation, English
  17. Relationship between nuclear dynamics and gene expression noise in pluripotent stem cell, Hiroshi Ochiai, The 68th Annual Meeting of the Japan Society for Cell Biology, 2016/06/16, Without Invitation, English
  18. Active nuclear dynamics promotes heterochromatin clustering, Hiroshi Ochiai, The 4th International Symposium of the Mathematics on Chromatin Live Dynamics, 2015/12/09, With Invitation, English
  19. Simultaneous live imaging of a specific gene's transcription and dynamics., Ochiai H., Sugawara T. & Yamamoto T., BMB2015, 2015/12/03, Without Invitation, English
  20. Simultaneous live imaging of the transcription and nuclear position of specific genes, Hiroshi Ochiai, 2015 TIC (Transcription Imaging Consortium) meeting, Janelia Research Campus (VA), USA, 2015/09/10, Without Invitation, English, TIC, Janelia Research Campus (VA), USA
  21. Simultaneous Live Imaging of the Transcription and Nuclear Position of Specific Genes, Hiroshi Ochiai, International Symposium on Chromatin Structure, Dynamics, and Function, Awaji, Japan, 2015/08/23, With Invitation, English, Awaji Yumebutai International Conference Center Awaji, Japan
  22. Simultaneous live-imaging of gene position and its transcriptional activity in mouse embryonic stem cells, Hiroshi Ochiai, Takeshi Sugawara, Takashi Yamamoto, 4D nucleome, Hiroshima, 2014/12/18, With Invitation, English
  23. Stochastic promoter activation affects gene expression variability in murine embryonic stem cells, Hiroshi Ochiai, Takeshi Sugawara, Tetsushi Sakuma, Takashi Yamamoto, CSHL meeting, NUCLEAR ORGANIZATION & FUNCTION, CSHL, NY, 2014/08/19, With Invitation, English, Cold Spring Harbor Laboratory Meeting & Courses, CSHL, NY
  24. TALEN-mediated single-base-pair editing in PCS syndrome, Hiroshi Ochiai, 3rd International Genome Engineering & Genome Editing-2014 Meeting on 'Synthetic Biology to Zinc Finger Nucleases & CRISPRs Regulation', Waltham, MA(USA), 2014/05/05, Without Invitation, English, Waltham, MA(USA)
  25. TALEN-mediated single-base-pair editing reveals the functional significance of an intergenic single nucleotide variant, Hiroshi Ochiai, International Symposium on RNAi and Genome Editing Research, Tokushima University, 2014/03/14, Without Invitation, English, Tokushima, Japan

Awards

  1. 2016/11, Hiroshima University President Award
  2. 2008/06, Hiroshima University Excellent Student Scholarship
  3. 2007/03, Faculty of Science Commendation

Patented

  1. Patent
  2. Patent

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, 2019/06/28, 2021/03/31
  2. KAKENHI, 2019/04/01, 2022/03/31
  3. Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 2018/06/29, 2023/03/31
  4. Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area), 2016/04/01, 2018/03/31
  5. PRESTO, 2015/10, 2019/03
  6. KAKENHI, 2015/04/01, 2018/03/31
  7. 2015/04, 2016/03
  8. Stochastic promoter activation affects Nanog expression variability in mouse embryonic stem cells, 2015, 2015
  9. 2014/10, 2017/03
  10. KAKENHI, 2013/04/01, 2015/03/31
  11. 2013, 2013
  12. 2013, 2013
  13. KAKENHI, 2011, 2013
  14. KAKENHI, 2009, 2010

Social Activities

History as Committee Members

  1. Editorial Board Member, 2015/07, 2021/05