鈴木 賢一KENICHI SUZUKI
Last Updated :2021/03/02
- 所属・職名
- 基礎生物学研究所 特任准教授
- メールアドレス
- suzuk107
hiroshima-u.ac.jp
研究活動
学術論文(★は代表的な論文)
- Amiodarone bioconcentration and suppression of metamorphosis in Xenopus., Aquatic toxicology (Amsterdam, Netherlands), 228巻, pp. 105623-105623, 20200906
- A simple and practical workflow for genotyping of CRISPR-Cas9-based knockout phenotypes using multiplexed amplicon sequencing., Genes to cells : devoted to molecular & cellular mechanisms, 20200423
- Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung., PloS one, 15巻, 10号, pp. e0240333, 2020
- In vitro assessment of effects of persistent organic pollutants on the transactivation of estrogen receptor α and β (ERα and ERβ) from the Baikal seal (Pusa sibirica)., Ecotoxicology and environmental safety, 181巻, pp. 463-471, 201910
- A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology., DNA research : an international journal for rapid publication of reports on genes and genomes, 26巻, 3号, pp. 217-229, 20190601
- Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration., Developmental biology, 443巻, 2号, pp. 127-136, 20181115
- A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair., Methods in molecular biology (Clifton, N.J.), 1865巻, pp. 91-103, 2018
- Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas, Biology Open, 7巻, 1号, pp. bio030338, 2018
- Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration, DEVELOPMENTAL BIOLOGY, 432巻, 2号, pp. 265-272, 201712
- Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin, JOURNAL OF CELL SCIENCE, 130巻, 24号, pp. 4097-4107, 201712
- Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis, JOURNAL OF TOXICOLOGICAL SCIENCES, 42巻, 5号, pp. 605-613, 201710
- Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis, DEVELOPMENTAL BIOLOGY, 426巻, 2号, pp. 384-392, 201706
- A simple protocol for loss-of-function analysis in Xenopus tropicalis founders using the CRISPR-Cas system, Methods in Molecular Biology, 1630巻, pp. 189-203, 2017
- Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders, GENES TO CELLS, 21巻, 7号, pp. 755-771, 201607
- Cilia play a role in breaking left–right symmetry of the sea urchin embryo, Genes to Cells, 21巻, 6号, pp. 568-578, 201606
- In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21巻, 4号, pp. 358-369, 201604
- MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11巻, 1号, pp. 118-133, 201601
- The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10巻, 11号, 201511
- Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity, ARCHIVES OF TOXICOLOGY, 89巻, 11号, pp. 2027-2038, 201511
- Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156巻, 2号, pp. 735-744, 201502
- Genome editing using site-specific nucleases in amphibians, Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System, pp. 133-149, 20150101
- Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5巻, 201411
- Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp. 108-114, 201401
- Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63巻, 1号, pp. 79-84, 201401
- Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, Scientific Reports, 3巻, 20131129
- High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, BIOLOGY OPEN, 2巻, 5号, pp. 448-452, 201305
- Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18巻, 4号, pp. 315-326, 201304
- Efficient TALEN construction and evaluation methods for human cell and animal applications., Genes to cells : devoted to molecular & cellular mechanisms, 18巻, 4号, 2013
- Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109巻, 27号, pp. 10915-10920, 201207
- Spatiotemporal expression profile of no29/nucleophosmin3 in the intestine of Xenopus laevis during metamorphosis, CELL AND TISSUE RESEARCH, 344巻, 3号, pp. 445-453, 201106
- Characterization of a Novel Type I Keratin Gene and Generation of Transgenic Lines With Fluorescent Reporter Genes Driven by Its Promoter/Enhancer in Xenopus laevis, DEVELOPMENTAL DYNAMICS, 239巻, 12号, pp. 3172-3181, 201012
- Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15巻, 8号, pp. 875-885, 201008
- Molecular features of thyroid hormone-regulated skin remodeling in Xenopus laevis during metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 51巻, 4号, pp. 411-427, 200905
- Disruption of Thyroid Hormone Function by Environmental Pollutants, JOURNAL OF HEALTH SCIENCE, 55巻, 2号, pp. 147-160, 200904
- The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237巻, 9号, pp. 2475-2482, 200809
- Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells, DEVELOPMENT GROWTH & DIFFERENTIATION, 50巻, 5号, pp. 307-320, 200806
- Characterization of histone lysine-specific demethylase in relation to thyroid hormone-regulated anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49巻, 4号, pp. 325-334, 200705
- Shh/BMP-4 signaling pathway is essential for intestinal epithelial development during Xenopus larval-to-adult remodeling, DEVELOPMENTAL DYNAMICS, 235巻, 12号, pp. 3240-3249, 200612
- Molecular identification of the skin transformation center of anuran larval skin using genes of Rana adult keratin (RAK) and SPARC as probes, DEVELOPMENT GROWTH & DIFFERENTIATION, 45巻, 5-6号, pp. 515-526, 200310
- Metamorphosis-dependent transcriptional regulation of xak-c, a novel Xenopus type I keratin gene, DEVELOPMENTAL DYNAMICS, 225巻, 4号, pp. 561-570, 200212
- Lineage of anuran epidermal basal cells and their differentiation potential in relation to metamorphic skin remodeling, DEVELOPMENT GROWTH & DIFFERENTIATION, 44巻, 3号, pp. 225-238, 200206
- Novel Rana keratin genes and their expression during larval to adult epidermal conversion in bullfrog tadpoles., Differentiation; research in biological diversity, 68巻, 1号, pp. 44-54, 200108
- Thyroid-hormone-dependent and fibroblast-specific expression of BMP-4 correlates with adult epithelial development during amphibian intestinal remodeling, CELL AND TISSUE RESEARCH, 303巻, 2号, pp. 187-195, 200102
- New epidermal keratin genes from Xenopus laevis: hormonal and regional regulation of their expression during anuran skin metamorphosis, BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1517巻, 3号, pp. 339-350, 200102
- Developmentally and regionally regulated participation of epidermal cells in the formation of collagen lamella of anuran tadpole skin, DEVELOPMENT GROWTH & DIFFERENTIATION, 42巻, 6号, pp. 571-580, 200012
著書等出版物
- 2016年, ゲノム編集入門 : ZFN・TALEN・CRISPR-Cas9, ゲノム編集入門 : ZFN・TALEN・CRISPR-Cas9, 裳華房, 2016年, jpn, 山本 卓; 石野 良純; 近藤 昭彦; 西田 敬二; 荒添 貴之; 丹羽 隆介; 坂本 尚昭; 泰松 清人; 川原 敦雄; 鈴木 賢一; 宮坂 佳樹; 真下 知士; 安本 周平; 村中 俊哉; 宮本 達雄; 田中 伸和, 9784785358662
- 2015年, ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意, ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意, 羊土社, 2015年, jpn, 山本 卓; 佐久間 哲史; 落合 博; 李 紅梅; 堀田 秋津; 藤原 祥高; 伊川 正人; 真下 知士; 鈴木 賢一; 川原 敦雄; 木下 政人; 笹倉 靖徳; 林 茂生; 大門 高明; 杉 拓磨; 安本 周平; 關 光; 刑部 祐里子; 刑部 敬史; 村中 俊哉, 9784758101936
受賞
- 2015年11月13日, 第14回広島大学長表彰
外部資金
競争的資金等の採択状況
- JST-CREST, 組織変形動態解析を起点とした種間・器官間で保存された形態形成則の解明, 2020年, 2025年
- 内藤記念科学振興財団, ネッタイツメガエルとゲノム編集技術を駆使した疾患・病態モデル動物の作製, 2016年
- 内藤記念科学振興財団, 表皮発生における表皮幹細胞の分化機構, 2007年
- 科学研究費補助金 若手研究(B), 分子から個体レベルまで総合的に内分泌攪乱物質を評価できるセンサーガエルの開発, 2008年, 2010年
- 科学研究費補助金 基盤研究(C), ツメガエル視床下部-下垂体-副腎及び甲状腺軸かく乱の作用機序と試験評価系の確立, 2012年, 2014年
- 新学術領域研究(研究領域提案型) 公募研究, エピゲノム編集技術を用いた再生原理の解明:失われた再生能力を回復させる試み, 2013年, 2015年
- 科学研究費補助金 基盤研究(C), ゲノム編集技術を用いた両生類の高度モデル動物化のために必要なストラテジーの確立, 2015年, 2017年
- 花王メラニン研究会 第2回研究助成, ゲノム編集技術を用いたメラニン生合成系遺伝子群の機能的スクリーニングとその薬理評価モデルの開発, 2016年
- 科学研究費補助金 基盤研究(C), 器官再生を惹起するROSシグナルの解明, 2018年, 2021年
社会活動
委員会等委員歴
- 第3期NBRPネッタイツメガエル 運営委員, 2015年
- 第4期NBRPネッタイツメガエル 運営委員, 2017年