鈴木 賢一KENICHI SUZUKI

Last Updated :2021/02/05

所属・職名
基礎生物学研究所　特任准教授

基本情報

学歴

  • 広島大学, 大学院理学研究科

学位

  • 博士（理学）　(広島大学)

研究分野

  • 生物学 / 生物科学 / 発生生物学

研究キーワード

  • 発生生物学、再生生物学、ゲノム編集、両生類

所属学会

  • 日本発生生物学会、日本動物学会、ゲノム編集学会

研究活動

学術論文（★は代表的な論文）

  1. Amiodarone bioconcentration and suppression of metamorphosis in Xenopus., Aquatic toxicology (Amsterdam, Netherlands), 228巻, pp. 105623-105623, 20200906
  2. A simple and practical workflow for genotyping of CRISPR-Cas9-based knockout phenotypes using multiplexed amplicon sequencing., Genes to cells : devoted to molecular & cellular mechanisms, 20200423
  3. Fgf10-CRISPR mosaic mutants demonstrate the gene dose-related loss of the accessory lobe and decrease in the number of alveolar type 2 epithelial cells in mouse lung., PloS one, 15巻, 10号, pp. e0240333, 2020
  4. In vitro assessment of effects of persistent organic pollutants on the transactivation of estrogen receptor α and β (ERα and ERβ) from the Baikal seal (Pusa sibirica)., Ecotoxicology and environmental safety, 181巻, pp. 463-471, 201910
  5. A comprehensive reference transcriptome resource for the Iberian ribbed newt Pleurodeles waltl, an emerging model for developmental and regeneration biology., DNA research : an international journal for rapid publication of reports on genes and genomes, 26巻, 3号, pp. 217-229, 20190601
  6. Cas9 ribonucleoprotein complex allows direct and rapid analysis of coding and noncoding regions of target genes in Pleurodeles waltl development and regeneration., Developmental biology, 443巻, 2号, pp. 127-136, 20181115
  7. A Simple Knock-In System for Xenopus via Microhomology Mediated End Joining Repair., Methods in molecular biology (Clifton, N.J.), 1865巻, pp. 91-103, 2018
  8. Functional analysis of thyroid hormone receptor beta in Xenopus tropicalis founders using CRISPR-Cas, Biology Open, 7巻, 1号, pp. bio030338, 2018
  9. Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration, DEVELOPMENTAL BIOLOGY, 432巻, 2号, pp. 265-272, 201712
  10. Dynamic changes in the interchromosomal interaction of early histone gene loci during development of sea urchin, JOURNAL OF CELL SCIENCE, 130巻, 24号, pp. 4097-4107, 201712
  11. Developmental changes in drug-metabolizing enzyme expression during metamorphosis of Xenopus tropicalis, JOURNAL OF TOXICOLOGICAL SCIENCES, 42巻, 5号, pp. 605-613, 201710
  12. Clustered Xenopus keratin genes: A genomic, transcriptomic, and proteomic analysis, DEVELOPMENTAL BIOLOGY, 426巻, 2号, pp. 384-392, 201706
  13. A simple protocol for loss-of-function analysis in Xenopus tropicalis founders using the CRISPR-Cas system, Methods in Molecular Biology, 1630巻, pp. 189-203, 2017
  14. Rapid and efficient analysis of gene function using CRISPR-Cas9 in Xenopus tropicalis founders, GENES TO CELLS, 21巻, 7号, pp. 755-771, 201607
  15. Cilia play a role in breaking left–right symmetry of the sea urchin embryo, Genes to Cells, 21巻, 6号, pp. 568-578, 201606
  16. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration, GENES TO CELLS, 21巻, 4号, pp. 358-369, 201604
  17. MMEJ-assisted gene knock-in using TALENs and CRISPR-Cas9 with the PITCh systems, NATURE PROTOCOLS, 11巻, 1号, pp. 118-133, 201601
  18. The Expression of TALEN before Fertilization Provides a Rapid Knock-Out Phenotype in Xenopus laevis Founder Embryos, PLOS ONE, 10巻, 11号, 201511
  19. Novel role of hnRNP-A2/B1 in modulating aryl hydrocarbon receptor ligand sensitivity, ARCHIVES OF TOXICOLOGY, 89巻, 11号, pp. 2027-2038, 201511
  20. Unliganded Thyroid Hormone Receptor alpha Regulates Developmental Timing via Gene Repression in Xenopus tropicalis, ENDOCRINOLOGY, 156巻, 2号, pp. 735-744, 201502
  21. Genome editing using site-specific nucleases in amphibians, Targeted Genome Editing Using Site-Specific Nucleases: ZFNs, TALENs, and the CRISPR/Cas9 System, pp. 133-149, 20150101
  22. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9, NATURE COMMUNICATIONS, 5巻, 201411
  23. Targeted mutagenesis of multiple and paralogous genes in Xenopus laevis using two pairs of transcription activator-like effector nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 56巻, 1号, pp. 108-114, 201401
  24. Screening Methods to Identify TALEN-Mediated Knockout Mice, EXPERIMENTAL ANIMALS, 63巻, 1号, pp. 79-84, 201401
  25. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity, Scientific Reports, 3巻, 20131129
  26. High efficiency TALENs enable F0 functional analysis by targeted gene disruption in Xenopus laevis embryos, BIOLOGY OPEN, 2巻, 5号, pp. 448-452, 201305
  27. Efficient TALEN construction and evaluation methods for human cell and animal applications, GENES TO CELLS, 18巻, 4号, pp. 315-326, 201304
  28. Efficient TALEN construction and evaluation methods for human cell and animal applications., Genes to cells : devoted to molecular & cellular mechanisms, 18巻, 4号, 2013
  29. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 109巻, 27号, pp. 10915-10920, 201207
  30. Spatiotemporal expression profile of no29/nucleophosmin3 in the intestine of Xenopus laevis during metamorphosis, CELL AND TISSUE RESEARCH, 344巻, 3号, pp. 445-453, 201106
  31. Characterization of a Novel Type I Keratin Gene and Generation of Transgenic Lines With Fluorescent Reporter Genes Driven by Its Promoter/Enhancer in Xenopus laevis, DEVELOPMENTAL DYNAMICS, 239巻, 12号, pp. 3172-3181, 201012
  32. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases, GENES TO CELLS, 15巻, 8号, pp. 875-885, 201008
  33. Molecular features of thyroid hormone-regulated skin remodeling in Xenopus laevis during metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 51巻, 4号, pp. 411-427, 200905
  34. Disruption of Thyroid Hormone Function by Environmental Pollutants, JOURNAL OF HEALTH SCIENCE, 55巻, 2号, pp. 147-160, 200904
  35. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo, DEVELOPMENTAL DYNAMICS, 237巻, 9号, pp. 2475-2482, 200809
  36. Identification and characterization of nucleoplasmin 3 as a histone-binding protein in embryonic stem cells, DEVELOPMENT GROWTH & DIFFERENTIATION, 50巻, 5号, pp. 307-320, 200806
  37. Characterization of histone lysine-specific demethylase in relation to thyroid hormone-regulated anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49巻, 4号, pp. 325-334, 200705
  38. Shh/BMP-4 signaling pathway is essential for intestinal epithelial development during Xenopus larval-to-adult remodeling, DEVELOPMENTAL DYNAMICS, 235巻, 12号, pp. 3240-3249, 200612
  39. Molecular identification of the skin transformation center of anuran larval skin using genes of Rana adult keratin (RAK) and SPARC as probes, DEVELOPMENT GROWTH & DIFFERENTIATION, 45巻, 5-6号, pp. 515-526, 200310
  40. Metamorphosis-dependent transcriptional regulation of xak-c, a novel Xenopus type I keratin gene, DEVELOPMENTAL DYNAMICS, 225巻, 4号, pp. 561-570, 200212
  41. Lineage of anuran epidermal basal cells and their differentiation potential in relation to metamorphic skin remodeling, DEVELOPMENT GROWTH & DIFFERENTIATION, 44巻, 3号, pp. 225-238, 200206
  42. Novel Rana keratin genes and their expression during larval to adult epidermal conversion in bullfrog tadpoles., Differentiation; research in biological diversity, 68巻, 1号, pp. 44-54, 200108
  43. Thyroid-hormone-dependent and fibroblast-specific expression of BMP-4 correlates with adult epithelial development during amphibian intestinal remodeling, CELL AND TISSUE RESEARCH, 303巻, 2号, pp. 187-195, 200102
  44. New epidermal keratin genes from Xenopus laevis: hormonal and regional regulation of their expression during anuran skin metamorphosis, BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1517巻, 3号, pp. 339-350, 200102
  45. Developmentally and regionally regulated participation of epidermal cells in the formation of collagen lamella of anuran tadpole skin, DEVELOPMENT GROWTH & DIFFERENTIATION, 42巻, 6号, pp. 571-580, 200012

著書等出版物

  1. 2016年, ゲノム編集入門 : ZFN・TALEN・CRISPR-Cas9, ゲノム編集入門 : ZFN・TALEN・CRISPR-Cas9, 裳華房, 2016年, jpn, 山本 卓; 石野 良純; 近藤 昭彦; 西田 敬二; 荒添 貴之; 丹羽 隆介; 坂本 尚昭; 泰松 清人; 川原 敦雄; 鈴木 賢一; 宮坂 佳樹; 真下 知士; 安本 周平; 村中 俊哉; 宮本 達雄; 田中 伸和, 9784785358662
  2. 2015年, ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意, ゲノム編集成功の秘訣Q&A : 論文だけではわからない : TALEN、CRISPR/Cas9の極意, 羊土社, 2015年, jpn, 山本 卓; 佐久間 哲史; 落合 博; 李 紅梅; 堀田 秋津; 藤原 祥高; 伊川 正人; 真下 知士; 鈴木 賢一; 川原 敦雄; 木下 政人; 笹倉 靖徳; 林 茂生; 大門 高明; 杉 拓磨; 安本 周平; 關 光; 刑部 祐里子; 刑部 敬史; 村中 俊哉, 9784758101936

受賞

  1. 2015年11月13日, 第14回広島大学長表彰
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外部資金

競争的資金等の採択状況

  1. JST-CREST, 組織変形動態解析を起点とした種間・器官間で保存された形態形成則の解明, 2020年, 2025年
  2. 内藤記念科学振興財団, ネッタイツメガエルとゲノム編集技術を駆使した疾患・病態モデル動物の作製, 2016年
  3. 内藤記念科学振興財団, 表皮発生における表皮幹細胞の分化機構, 2007年
  4. 科学研究費補助金　若手研究(B), 分子から個体レベルまで総合的に内分泌攪乱物質を評価できるセンサーガエルの開発, 2008年, 2010年
  5. 科学研究費補助金　基盤研究(C), ツメガエル視床下部-下垂体-副腎及び甲状腺軸かく乱の作用機序と試験評価系の確立, 2012年, 2014年
  6. 新学術領域研究(研究領域提案型)　公募研究, エピゲノム編集技術を用いた再生原理の解明：失われた再生能力を回復させる試み, 2013年, 2015年
  7. 科学研究費補助金　基盤研究(C), ゲノム編集技術を用いた両生類の高度モデル動物化のために必要なストラテジーの確立, 2015年, 2017年
  8. 花王メラニン研究会　第２回研究助成, ゲノム編集技術を用いたメラニン生合成系遺伝子群の機能的スクリーニングとその薬理評価モデルの開発, 2016年
  9. 科学研究費補助金　基盤研究(C), 器官再生を惹起するROSシグナルの解明, 2018年, 2021年

社会活動

委員会等委員歴

  1. 第3期NBRPネッタイツメガエル 運営委員, 2015年
  2. 第4期NBRPネッタイツメガエル 運営委員, 2017年