島田 昌之Masayuki Shimada

Last Updated :2018/09/03

所属・職名
大学院生物圏科学研究科 陸域動物生産学講座 教授
メールアドレス
mashimadhiroshima-u.ac.jp

基本情報

学位

  • 博士(獣医学) (山口大学)
  • 修士(農学) (広島大学)

研究分野

  • 農学 / 動物生命科学 / 動物生産科学

研究キーワード

  • 胚発生|ラット
  • 卵丘細胞|プロジェステロン
  • 卵子|排卵
  • 体外受精
  • 体外成熟
  • クローン
  • 卵子
  • 卵巣
  • 不妊治療
  • ブタ卵子

教育活動

授業担当

  1. 2018年, 教養教育, 4ターム, 生物生産と自然との関わり
  2. 2018年, 教養教育, セメスター(前期), 食文化論
  3. 2018年, 学部専門, 集中, 基礎生物学実験I
  4. 2018年, 学部専門, 集中, 基礎生物学実験II
  5. 2018年, 学部専門, 2ターム, 動物生産サイエンス入門
  6. 2018年, 学部専門, 3ターム, 動物生殖学
  7. 2018年, 学部専門, 集中, 動物生殖学実験実習
  8. 2018年, 学部専門, セメスター(前期), 発生工学
  9. 2018年, 学部専門, セメスター(後期), 実験動物学
  10. 2018年, 学部専門, 集中, 海外生物生産学演習I
  11. 2018年, 学部専門, 集中, 海外生物生産学演習II
  12. 2018年, 学部専門, 集中, 海外生物生産学演習III
  13. 2018年, 学部専門, 集中, 国際課題研究
  14. 2018年, 修士課程・博士課程前期, セメスター(前期), 家畜生殖細胞利用学実験実習
  15. 2018年, 修士課程・博士課程前期, 1ターム, 分子内分泌学
  16. 2018年, 修士課程・博士課程前期, 3ターム, 動物生殖細胞学
  17. 2018年, 修士課程・博士課程前期, セメスター(後期), 特別研究
  18. 2018年, 修士課程・博士課程前期, セメスター(前期), 陸域動物生産学演習(A)
  19. 2018年, 修士課程・博士課程前期, セメスター(後期), 陸域動物生産学演習(A)
  20. 2018年, 博士課程・博士課程後期, セメスター(前期), 陸域動物生産学演習(B)
  21. 2018年, 博士課程・博士課程後期, セメスター(後期), 陸域動物生産学演習(B)
  22. 2018年, 修士課程・博士課程前期, セメスター(前期), 陸域動物生産学実験実習

研究活動

学術論文(★は代表的な論文)

  1. A Role for PI 3-Kinase in bovine oocytes nuclear maturation., Theriogenology, 50巻, 7号, pp.347-pp.356, 19980401
  2. Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from MⅠ to MⅡ Stage in Porcine Follicular Oocytes., J Mamm Ova Res, 15巻, 3号, pp.68-pp.76, 19980401
  3. The production of progesterone from de novo-synthesized cholesterol in cumulus cells, and its physiological role during meiotic resumption of porcine oocytes, Biology of Reproduction, 68巻, 4号, pp.1193-pp.1198, 20030401
  4. Effects of estrone sulfate injection on reproductive functions in male Japanese quail., Journal of Poultry Science, 40巻, pp.243-pp.247, 20031001
  5. PI 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes., Journal of Endocrinology, 179巻, pp.25-pp.34, 20031001
  6. Effect of Protein kinase C activator on mitogen-activated protein kinase and p34cdc2 kinase activity during parthenogenetic activation of porcine oocytes by calcium ionophore., Biology of Reproduction, 69巻, pp.1675-pp.1682, 20031101
  7. ★, Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002 on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes., J Reprod Dev, 44巻, 5号, pp.281-pp.288, 19980401
  8. Roles of cAMP in Regulation of both MAP kinase and p34cdc2 kinase activity during Meiotic Progression,especially beyond the MI stage., Molecular Reproduction and Development, 62巻, 1号, pp.124-pp.131, 20020501
  9. ★, FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes., Molecular Human Reproduction, 8巻, pp.621-pp.628, 20020701
  10. Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media., Reproduction, 124巻, pp.557-pp.564, 20021001
  11. Both Ca2+-PKC pathway and cAMP-PKA pathway require for progesterone production in FSH- and LH-stimulated cumulus cells during in vitro maturation of porcine oocytes., J Mamm Ova Res, 19巻, 3号, pp.81-pp.88, 20021001
  12. LH receptor formation in cumulus cells surrounding porcine oocytes, and its role during meiotic maturation of porcine oocytes., Biology of Reproduction, 68巻, 4号, pp.1142-pp.1149, 20030401
  13. Selective expression of Kras(G12D) in granulosa cells of the mouse ovary causes defects in follicle development and ovulation, DEVELOPMENT, 135巻, 12号, pp.2127-pp.2137, JUN 15 2008
  14. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization, DEVELOPMENT, 135巻, 11号, pp.2001-pp.2011, JUN 1 2008
  15. The involvement of the Toll-like receptor family in ovulation, JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 25巻, 6号, pp.223-pp.228, 42894
  16. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, REPRODUCTION, 136巻, 1号, pp.9-pp.21, 42924
  17. Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs, JOURNAL OF OVARIAN RESEARCH, 2巻, 1号, 2009
  18. Immune-like mechanisms in ovulation, TRENDS IN ENDOCRINOLOGY AND METABOLISM, 19巻, 6号, pp.191-pp.196, 42955
  19. Seminal plasma damages sperm during cryopreservation, but its presence during thawing improves semen quality and conception rates in boars with poor post-thaw semen quality, THERIOGENOLOGY, 71巻, 3号, pp.491-pp.498, 42775
  20. Improved conception rates in sows inseminated with cryopreserved boar spermatozoa prepared with a more optimal combination of osmolality and glycerol in the freezing extender, ANIMAL SCIENCE JOURNAL, 80巻, 2号, pp.121-pp.129, 42834
  21. MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility, SCIENCE, 324巻, 5929号, pp.938-pp.941, MAY 15 2009
  22. Interleukin-6: An Autocrine Regulator of the Mouse Cumulus Cell-Oocyte Complex Expansion Process, ENDOCRINOLOGY, 150巻, 7号, pp.3360-pp.3368, 42925
  23. Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro, ANIMAL SCIENCE JOURNAL, 81巻, 1号, pp.42-pp.47, 2010
  24. beta-Catenin (CTNNB1) Promotes Preovulatory Follicular Development but Represses LH-Mediated Ovulation and Luteinization, MOLECULAR ENDOCRINOLOGY, 24巻, 8号, pp.1529-pp.1542, 42957
  25. Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation, THERIOGENOLOGY, 74巻, 9号, pp.1691-pp.1700, 43079
  26. LH-Induced Neuregulin 1 (NRG1) Type III Transcripts Control Granulosa Cell Differentiation and Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 25巻, 1号, pp.104-pp.116, 42746
  27. The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm, ANIMAL SCIENCE JOURNAL, 82巻, 3号, pp.412-pp.419, 2011
  28. Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis, HUMAN REPRODUCTION, 26巻, 10号, pp.2799-pp.2806, 43019
  29. Positive Feedback Loop Between Prostaglandin E2 and EGF-Like Factors Is Essential for Sustainable Activation of MAPK3/1 in Cumulus Cells During In Vitro Maturation of Porcine Cumulus Oocyte Complexes, BIOLOGY OF REPRODUCTION, 85巻, 5号, pp.1073-pp.1082, 43050
  30. Dynamic changes of connexin-43, gap junctional protein, in outer layers of cumulus cells are regulated by PKC and PI 3-kinase during meiotic resumption in porcine oocytes, Biology of Reproduction, 64巻, 4号, pp.1255-pp.1263, 20010401
  31. PI 3-kinase in cumulus cells and oocytes is responsible for activation of oocyte MAP kinase during meiotic progression beyond the MI stage in pigs, Biology of Reproduction, 64巻, 4号, pp.1106-pp.1114, 20010401
  32. The drop of cAMP level due to the closure of gap junctional communication between cumulus cells and oocyte is essential for meiotic progression beyond the MI stage in porcine oocytes, J Mamm Ova Res, 18巻, 3号, pp.99-pp.105, 20010401
  33. Progression of nuclear maturation and p34cdc2 kinase activity in porcine oocytes during in vitro culture in different medium, J Mamm Ova Res, 18巻, 1号, pp.39-pp.43, 20010401
  34. Survival of Boar Spermatozoa Frozen in Diluents of Varying Osmolality., Theriogenology, 56巻, 8号, pp.447-pp.458, 20010401
  35. ★, Inhibition of PI 3-kinase or MEK Leads to Suppression of p34cdc2 kinase Activity and Meiotic Progression beyond the MI stage in Porcine Oocyte Surrounded with Cumulus Cells., Biology of Reproduction, 65巻, 2号, pp.442-pp.448, 20010401
  36. ★, Dynamic Changes of Connexin-43, Gap Junctional Protein, in Outer Layers of Cumulus Cells are Regulated by PKC and PI 3-Kinase during Meiotic Resumption in Porcine Oocytes., Biology of Reproduction, 64巻, 4号, pp.1255-pp.1263, 20010401
  37. ★, PI 3-kinase in Cumulus Cells and Oocytes is Responsible for Activation of Oocyte MAP kinase during Meiotic Progression beyond the MI stage in Pigs., Biology of Reproduction, 64巻, 4号, pp.1106-pp.1114, 20010401
  38. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, blocks in vitro fertilization and embryonic development of bovine oocytes matured in vitro with epidermal growth factor., J Mamm Ova Res, 17巻, 3号, pp.109-pp.114, 20000401
  39. Meiotic Maturation of Bovine Oocytes Cultured In vitro with EGF and Wortmannin., J Mamm Ova Res, 17巻, 3号, pp.103-pp.108, 20000401
  40. Activation by stimuli of sperm penetration in bovine oocytes treated with high concentration of BAPTA/AM., J Mamm Ova Res, 17巻, 3号, pp.96-pp.102, 20000401
  41. Effects of wortmannin on the kinetics of GVBD and the activities of the maturation-promoting factor and mitogen-activated protein kinase during bovine oocyte maturation in vitro, Theriogenology, 53巻, 5号, pp.1797-pp.1806, 20000401
  42. Phosphorylation of Connexin-43, Gap Junctional Protein, in Cumulus Cells Are Regulated by Mitogen Activated Protein Kinase and Phosphatidylinositol 3-Kinase during In Vitro Meiotic Resumption in Porcine Follicular Oocytes., J Mamm Ova Res, 16巻, 3号, pp.37-pp.42, 19990401
  43. LH reduces proliferative activity of cumulus cells and accelerates GVBD of porcine oocytes., Molecular and Cellular Endocrinology, 209巻, pp.43-pp.50, 20031201
  44. Time dependent changes in progesterone receptor expression in cumulus cells during meiotic resumption of porcine oocytes, Journal of Mammalian Ova Research, 20巻, pp.113-pp.117, 20031001
  45. Mitogen-Activated Protein Kinase Kinase Inhibitor Suppresses Cyclin B1 Synthesis and Reactivation of p34cdc2 Kinase, Which Improves Pronuclear Formation Rate in Matured Porcine Oocytes Activated by Ca2+ Ionophore, Biology of Reproduction, 70巻, pp.797-pp.804, 20040301
  46. Dramatic changese in motility and penetration competence of miniture pig spermatozoa frozen after exposure to seminal plasma, Theriogenology, 61巻, 42769号, pp.351-pp.364, 20040101
  47. Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes, Journal of Molecular Endocrinology, pp.in press, 20040401
  48. The role of Calcium/Calmodulin-dependent protein kinase II (CaMKII) on inactivation of MAP kinase and p34cdc2 kinase during fertilization and activation in pig oocytes, Reproduction, 128巻, pp.409-pp.415, 20040401
  49. Down-regulated expression of ADAMTS-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes, Endocrinology, 145巻, pp.4603-pp.4614, 20040401
  50. Effects of adding LH to FSH-containing medium on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes, Animal Science Journal, pp.in press, 20040401
  51. FSH-induced activation of PI 3-kinase-PKB pathway is essential for LH receptor formation in cumulus cells during meiotic resumption of porcine oocytes., Japanese Journal of Reproductive Endocrinology, pp.in press, 20040401
  52. Gonadotropin-induced delta 14-reductase and delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, Endocrinology, 146巻, pp.186-pp.194, 20050101
  53. Gonadotropin-induced ⊿14-reductase and ⊿7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, Endocrinology, 146巻, 1号, pp.186-pp.194, 20050101
  54. Effects of adding luteinizing hormone to a medium containing follicle stimulating hormone on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes, Animal Science Journal, 75巻, pp.515-pp.523, 20040401
  55. Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs, JOURNAL OF OVARIAN RESEARCH, 2巻, 1号, 2009
  56. Fecal progestagen and estrone during pregnancy in a giraffe: A case report, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53巻, 1号, pp.159-pp.164, 2007
  57. Hormone-induced expression of tumor necrosis factor alpha-converting enzyme/a disintegrin and metalloprotease-17 impacts porcine cumulus cell oocyte complex expansion and meiotic maturation via ligand activation of the epidermal growth factor receptor, ENDOCRINOLOGY, 148巻, 12号, pp.6164-pp.6175, 2007
  58. Sequential exposure of porcine cumulus cells to FSH and LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, BIOLOGY OF REPRODUCTION, pp.150-pp.150, 2008
  59. PGE2 and progesterone enhance EGFR activation in porcine cumulus cells via the induction of TACE/ADAM17 expression but not EGF like factors expression during oocyte maturation in vivo and in vitro, BIOLOGY OF REPRODUCTION, pp.150-pp.150, 2008
  60. Neuregulin 1 Is a Novel Oocyte Maturation Regulatory Factor Expressed in Granulosa Cells During the Ovulation Process in Mice., BIOLOGY OF REPRODUCTION, pp.59-pp.59, 2009
  61. ERK1/2 in Ovarian Granulosa Cells Are Essential for Female Fertility., BIOLOGY OF REPRODUCTION, pp.82-pp.82, 2009
  62. Progesterone is Essential for Maintenance of Tace/Adam17 mRNA Expression, But not EGF-like Factor, in Cumulus Cells, Which Enhances the EGF Receptor Signaling Pathway During In Vitro Maturation of Porcine COCs, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56巻, 3号, pp.315-pp.323, 2010
  63. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization, DEVELOPMENT, 135巻, 11号, pp.2001-pp.2011, 2008
  64. The Release of EGF Domain from EGF-like Factors by a Specific Cleavage Enzyme Activates the EGFR-MAPK3/1 Pathway in Both Granulosa Cells and Cumulus Cells During the Ovulation Process, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58巻, 5号, pp.510-pp.514, 2012
  65. Cumulus Cell-Oocyte Complex Expansion Is Regulated by Ca2+ Induced m-Calpain Activity Leading to Cumulus Cell Motility and Migration, BIOLOGY OF REPRODUCTION, 85巻, 2011
  66. MAP kinase およびPl 3-kinaseが卵丘細胞間ギャップ結合の構成因子Connexin-43のリン酸化をブタ卵胞卵子の減数分裂再開時に制御する, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 16巻, 1号, pp.37-pp.42, 19990401
  67. The involvement of the Toll-like receptor family in ovulation, JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 25巻, 6号, pp.223-pp.228, 2008
  68. ERK1^<-/-> ; ERK2^ ; Cyp19A1Cre mice を用いた卵胞内エストロジェン濃度低下と排卵機構の解析, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 26巻, 2号, 20090401
  69. ラット排卵卵子の減数分裂停止に関わるMos/MEK/MAPK系と p34^ kinase の相補的な機能, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 22巻, 2号, 20050401
  70. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, REPRODUCTION, 136巻, 1号, pp.9-pp.21, 2008
  71. Selective expression of constitutively active KrasG12D in granulosa cells of the mouse ovary causes defects in follicle development and ovulation, BIOLOGY OF REPRODUCTION, pp.127-pp.127, 2008
  72. MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility, SCIENCE, 324巻, 5929号, pp.938-pp.941, 2009
  73. Interleukin-6: An Autocrine Regulator of the Mouse Cumulus Cell-Oocyte Complex Expansion Process, ENDOCRINOLOGY, 150巻, 7号, pp.3360-pp.3368, 2009
  74. Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro, ANIMAL SCIENCE JOURNAL, 81巻, 1号, pp.42-pp.47, 2010
  75. Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation, THERIOGENOLOGY, 74巻, 9号, pp.1691-pp.1700, 2010
  76. LH-Induced Neuregulin 1 (NRG1) Type III Transcripts Control Granulosa Cell Differentiation and Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 25巻, 1号, pp.104-pp.116, 2011
  77. The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm, ANIMAL SCIENCE JOURNAL, 82巻, 3号, pp.412-pp.419, 2011
  78. Positive Feedback Loop Between Prostaglandin E2 and EGF-Like Factors Is Essential for Sustainable Activation of MAPK3/1 in Cumulus Cells During In Vitro Maturation of Porcine Cumulus Oocyte Complexes, BIOLOGY OF REPRODUCTION, 85巻, 5号, pp.1073-pp.1082, 2011
  79. Endogenous acetaldehyde toxicity during antral follicular development in the mouse ovary, REPRODUCTIVE TOXICOLOGY, 33巻, 3号, pp.322-pp.330, 2012
  80. EGF-Like Factors Induce Expansion of the Cumulus Cell-Oocyte Complexes by Activating Calpain-Mediated Cell Movement, ENDOCRINOLOGY, 153巻, 8号, pp.3949-pp.3959, 2012
  81. Adiponectin and its receptors modulate granulosa cell and cumulus cell functions, fertility, and early embryo development in the mouse and human, FERTILITY AND STERILITY, 98巻, 2号, pp.471-pp.+, 2012
  82. New strategies of boar sperm cryopreservation: Development of novel freezing and thawing methods with a focus on the roles of seminal plasma, ANIMAL SCIENCE JOURNAL, 83巻, 9号, pp.623-pp.629, 2012
  83. Artificial Insemination With Seminal Plasma Improves the Reproductive Performance of Frozen-Thawed Boar Epididymal Spermatozoa, JOURNAL OF ANDROLOGY, 33巻, 5号, pp.990-pp.998, 2012
  84. The Expression of Acetaldehyde Dehydrogenase (ALDH) Type I Family Members Is Required to Decrease of Endogenous Acetaldehyde Toxicity During Ovarian Follicular Development in Mouse and Human, BIOLOGY OF REPRODUCTION, 85巻, 2011
  85. Targeted Disruption of Nrg1 in Granulosa Cells Alters the Temporal Progression of Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 28巻, 5号, pp.706-pp.721, 2014
  86. 卵丘細胞のPI 3-kinase とMI以降のブタ卵子のp34^ kinase との関係, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 16巻, 2号, 19990401
  87. 異なる成熟培地で培養したブタ卵子のp34cdc2 kinase活性の変化と核相の進行, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 18巻, 1号, pp.39-pp.43, 20010401
  88. Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002, on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes, The Journal of reproduction and development, 44巻, 3号, pp.281-pp.288, 19980801

招待講演、口頭・ポスター発表等

  1. 採卵された(てしまった)未成熟卵を如何に培養するか?, 島田昌之, 日本受精着床学会, 2016年91月, 招待, 日本語, 長野県