島田 昌之Masayuki Shimada

Last Updated :2020/09/01

所属・職名
大学院統合生命科学研究科 教授
メールアドレス
mashimadhiroshima-u.ac.jp

基本情報

主な職歴

  • 2007年04月01日, 2017年03月31日, 広島大学, 大学院生物圏科学研究科, 准教授
  • 2017年04月01日, 2019年03月31日, 広島大学, 大学院生物圏科学研究科, 教授

学位

  • 博士(獣医学) (山口大学)
  • 修士(農学) (広島大学)

研究分野

  • 農学 / 動物生命科学 / 動物生産科学

研究キーワード

  • 卵子|排卵
  • 受精/ 体外受精
  • 体外成熟
  • 卵巣
  • 生殖補助技術(医療)
  • 精子形成
  • 精巣
  • 精子保存

教育活動

授業担当

  1. 2020年, 教養教育, 3ターム, 細胞科学[1生]
  2. 2020年, 教養教育, セメスター(前期), 食文化論
  3. 2020年, 学部専門, 集中, 動物生殖学
  4. 2020年, 学部専門, 年度, 卒業論文
  5. 2020年, 学部専門, 2ターム, 発生工学
  6. 2020年, 学部専門, 集中, 基礎生物学実験II
  7. 2020年, 学部専門, 1ターム, 生理学入門
  8. 2020年, 学部専門, 3ターム, 実験動物学
  9. 2020年, 学部専門, 4ターム, 分子細胞生物学
  10. 2020年, 学部専門, 4ターム, 生命物質分析学
  11. 2020年, 学部専門, 3ターム, 分子農学生命科学外書講読
  12. 2020年, 学部専門, 集中, 海外生物生産学演習I
  13. 2020年, 学部専門, 集中, 海外生物生産学演習II
  14. 2020年, 学部専門, 集中, 海外生物生産学演習III
  15. 2020年, 学部専門, 集中, 国際課題研究
  16. 2020年, 修士課程・博士課程前期, 2ターム, 分子内分泌学
  17. 2020年, 修士課程・博士課程前期, 4ターム, 動物生殖細胞学
  18. 2020年, 修士課程・博士課程前期, セメスター(後期), 特別研究
  19. 2020年, 博士課程・博士課程後期, セメスター(前期), 陸域動物生産学演習(B)
  20. 2020年, 博士課程・博士課程後期, セメスター(後期), 陸域動物生産学演習(B)
  21. 2020年, 修士課程・博士課程前期, 4ターム, 生命科学社会実装論
  22. 2020年, 修士課程・博士課程前期, 2ターム, 応用動物生命科学Ⅰ
  23. 2020年, 修士課程・博士課程前期, 4ターム, 応用動物生命科学Ⅱ
  24. 2020年, 修士課程・博士課程前期, 1ターム, 食品生命科学特別演習A
  25. 2020年, 修士課程・博士課程前期, 2ターム, 食品生命科学特別演習A
  26. 2020年, 修士課程・博士課程前期, 3ターム, 食品生命科学特別演習B
  27. 2020年, 修士課程・博士課程前期, 4ターム, 食品生命科学特別演習B
  28. 2020年, 修士課程・博士課程前期, 年度, 食品生命科学特別研究
  29. 2020年, 修士課程・博士課程前期, 1ターム, 生物資源科学特別演習A
  30. 2020年, 修士課程・博士課程前期, 2ターム, 生物資源科学特別演習A
  31. 2020年, 修士課程・博士課程前期, 3ターム, 生物資源科学特別演習B
  32. 2020年, 修士課程・博士課程前期, 4ターム, 生物資源科学特別演習B
  33. 2020年, 修士課程・博士課程前期, 年度, 生物資源科学特別研究
  34. 2020年, 博士課程・博士課程後期, セメスター(後期), 生物圏多文化セミナーB
  35. 2020年, 修士課程・博士課程前期, 4ターム, ゲノム機能学概論

研究活動

学術論文(★は代表的な論文)

  1. Sperm enter glands of preovulatory bovine endometrial explants and initiate inflammation, REPRODUCTION, 159巻, 2号, pp. 181-192, 202002
  2. Roadmap to pregnancy in the first 7 days post-insemination in the cow: Immune crosstalk in the corpus luteum, oviduct, and uterus, THERIOGENOLOGY, 150巻, pp. 313-320, 20200701
  3. Mitochondrial Protein Turnover Is Critical for Granulosa Cell Proliferation and Differentiation in Antral Follicles, JOURNAL OF THE ENDOCRINE SOCIETY, 3巻, 2号, pp. 324-339, 201902
  4. Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm, FRONTIERS IN PHYSIOLOGY, 10巻, 20190312
  5. TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro, PLOS ONE, 14巻, 4号, 20190417
  6. Cyclooxygenase-2 is acutely induced by CCAAT/enhancer-binding protein beta to produce prostaglandin E-2 and F-2 alpha following gonadotropin stimulation in Leydig cells, MOLECULAR REPRODUCTION AND DEVELOPMENT, 86巻, 7号, pp. 786-797, 201907
  7. Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm, PLOS BIOLOGY, 17巻, 8号, 201908
  8. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria, FREE RADICAL BIOLOGY AND MEDICINE, 141巻, pp. 159-171, 201909
  9. Induction of immune-related gene expression by seminal exosomes in the porcine endometrium, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 495巻, 1号, pp. 1094-1101, 20180101
  10. Inductions of granulosa cell luteinization and cumulus expansion are dependent on the fibronectin-integrin pathway during ovulation process in mice, PLOS ONE, 13巻, 2号, 20180208
  11. Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass, GENES TO CELLS, 23巻, 3号, pp. 136-145, 201803
  12. A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro, MOLECULAR REPRODUCTION AND DEVELOPMENT, 85巻, 3号, pp. 215-226, 201803
  13. Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells, SCIENTIFIC REPORTS, 8巻, 20180518
  14. Evidence that interferon-tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 500巻, 4号, pp. 879-884, 20180612
  15. The Cell Type-Specific Expression of Lhcgr in Mouse Ovarian Cells: Evidence for a DNA-Demethylation-Dependent Mechanism, ENDOCRINOLOGY, 159巻, 5号, pp. 2062-2074, 201805
  16. Creatine enhances the duration of sperm capacitation: a novel factor for improving in vitro fertilization with small numbers of sperm, HUMAN REPRODUCTION, 33巻, 6号, pp. 1117-1129, 201806
  17. Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: possible involvement of interferon tau as an intermediator, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 63巻, 4号, pp. 425-434, 2017
  18. The acceleration of reproductive aging in Nrg1(flox/flox);Cyp19-Cre female mice, AGING CELL, 16巻, 6号, pp. 1288-1299, 201712
  19. De Novo-Synthesized Retinoic Acid in Ovarian Antral Follicles Enhances FSH-Mediated Ovarian Follicular Cell Differentiation and Female Fertility, ENDOCRINOLOGY, 157巻, 5号, pp. 2160-2172, 2016
  20. The Novel Pig In Vitro Maturation System to Improve Developmental Competence of Oocytes Derived from Atretic Nonvascularized Follicles, BIOLOGY OF REPRODUCTION, 95巻, 4号, 20161001
  21. Neuregulin 1 Regulates Proliferation of Leydig Cells to Support Spermatogenesis and Sexual Behavior in Adult Mice, ENDOCRINOLOGY, 157巻, 12号, pp. 4899-4913, 2016
  22. Protein Kinase C (PKC) Increases TACE/ADAM17 Enzyme Activity in Porcine Ovarian Somatic Cells, Which Is Essential for Granulosa Cell Luteinization and Oocyte Maturation, ENDOCRINOLOGY, 155巻, 3号, pp. 1080-1090, 2014
  23. The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice, DEVELOPMENT, 141巻, 23号, pp. 4618-4627, 2014
  24. Targeted Disruption of Nrg1 in Granulosa Cells Alters the Temporal Progression of Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 28巻, 5号, pp. 706-721, 2014
  25. Anti-Bacterial Factors Secreted From Cumulus Cells of Ovulated COCs Enhance Sperm Capacitation During In Vitro Fertilization, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 69巻, 2号, pp. 168-179, 2013
  26. Androgen/androgen receptor pathway regulates expression of the genes for cyclooxygenase-2 and amphiregulin in periovulatory granulosa cells, MOLECULAR AND CELLULAR ENDOCRINOLOGY, 369巻, 42737号, pp. 42-51, 20130430
  27. 卵の成熟におけるEGF受容体の活性化と活性化因子, 生体の科学, 64巻, pp. 462-463, 201308
  28. New strategies of boar sperm cryopreservation: Development of novel freezing and thawing methods with a focus on the roles of seminal plasma, ANIMAL SCIENCE JOURNAL, 83巻, 9号, pp. 623-629, 2012
  29. Artificial Insemination With Seminal Plasma Improves the Reproductive Performance of Frozen-Thawed Boar Epididymal Spermatozoa, JOURNAL OF ANDROLOGY, 33巻, 5号, pp. 990-998, 2012
  30. Adiponectin and its receptors modulate granulosa cell and cumulus cell functions, fertility, and early embryo development in the mouse and human, FERTILITY AND STERILITY, 98巻, 2号, pp. 471-+, 2012
  31. The Release of EGF Domain from EGF-like Factors by a Specific Cleavage Enzyme Activates the EGFR-MAPK3/1 Pathway in Both Granulosa Cells and Cumulus Cells During the Ovulation Process, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58巻, 5号, pp. 510-514, 2012
  32. Endogenous acetaldehyde toxicity during antral follicular development in the mouse ovary, REPRODUCTIVE TOXICOLOGY, 33巻, 3号, pp. 322-330, 2012
  33. EGF-Like Factors Induce Expansion of the Cumulus Cell-Oocyte Complexes by Activating Calpain-Mediated Cell Movement, ENDOCRINOLOGY, 153巻, 8号, pp. 3949-3959, 2012
  34. The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm, ANIMAL SCIENCE JOURNAL, 82巻, 3号, pp. 412-419, 2011
  35. Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis, HUMAN REPRODUCTION, 26巻, 10号, pp. 2799-2806, 2011
  36. Positive Feedback Loop Between Prostaglandin E2 and EGF-Like Factors Is Essential for Sustainable Activation of MAPK3/1 in Cumulus Cells During In Vitro Maturation of Porcine Cumulus Oocyte Complexes, BIOLOGY OF REPRODUCTION, 85巻, 5号, pp. 1073-1082, 2011
  37. Cumulus Cell-Oocyte Complex Expansion Is Regulated by Ca2+ Induced m-Calpain Activity Leading to Cumulus Cell Motility and Migration, BIOLOGY OF REPRODUCTION, 85巻, 2011
  38. LH-Induced Neuregulin 1 (NRG1) Type III Transcripts Control Granulosa Cell Differentiation and Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 25巻, 1号, pp. 104-116, 2011
  39. Positive Feedback Loop Between Prostaglandin E2 and EGF-Like Factors Is Essential for Sustainable Activation of MAPK3/1 in Cumulus Cells During In Vitro Maturation of Porcine Cumulus Oocyte Complexes, BIOLOGY OF REPRODUCTION, 85巻, 5号, pp. 1073-1082, 2011
  40. The Expression of Acetaldehyde Dehydrogenase (ALDH) Type I Family Members Is Required to Decrease of Endogenous Acetaldehyde Toxicity During Ovarian Follicular Development in Mouse and Human, BIOLOGY OF REPRODUCTION, 85巻, 2011
  41. Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro, ANIMAL SCIENCE JOURNAL, 81巻, 1号, pp. 42-47, 2010
  42. beta-Catenin (CTNNB1) Promotes Preovulatory Follicular Development but Represses LH-Mediated Ovulation and Luteinization, MOLECULAR ENDOCRINOLOGY, 24巻, 8号, pp. 1529-1542, 2010
  43. Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation, THERIOGENOLOGY, 74巻, 9号, pp. 1691-1700, 2010
  44. Progesterone is Essential for Maintenance of Tace/Adam17 mRNA Expression, But not EGF-like Factor, in Cumulus Cells, Which Enhances the EGF Receptor Signaling Pathway During In Vitro Maturation of Porcine COCs, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56巻, 3号, pp. 315-323, 2010
  45. Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation, THERIOGENOLOGY, 74巻, 9号, pp. 1691-1700, 2010
  46. Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs, JOURNAL OF OVARIAN RESEARCH, 2巻, 1号, 2009
  47. MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility, SCIENCE, 324巻, 5929号, pp. 938-941, 20090515
  48. Interleukin-6: An Autocrine Regulator of the Mouse Cumulus Cell-Oocyte Complex Expansion Process, ENDOCRINOLOGY, 150巻, 7号, pp. 3360-3368, 2009
  49. Neuregulin 1 Is a Novel Oocyte Maturation Regulatory Factor Expressed in Granulosa Cells During the Ovulation Process in Mice., BIOLOGY OF REPRODUCTION, pp. 59-59, 2009
  50. ERK1/2 in Ovarian Granulosa Cells Are Essential for Female Fertility., BIOLOGY OF REPRODUCTION, pp. 82-82, 2009
  51. MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility, SCIENCE, 324巻, 5929号, pp. 938-941, 2009
  52. Interleukin-6: An Autocrine Regulator of the Mouse Cumulus Cell-Oocyte Complex Expansion Process, ENDOCRINOLOGY, 150巻, 7号, pp. 3360-3368, 2009
  53. Selective expression of Kras(G12D) in granulosa cells of the mouse ovary causes defects in follicle development and ovulation, DEVELOPMENT, 135巻, 12号, pp. 2127-2137, 20080615
  54. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization, DEVELOPMENT, 135巻, 11号, pp. 2001-2011, 20080601
  55. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, REPRODUCTION, 136巻, 1号, pp. 9-21, 2008
  56. Immune-like mechanisms in ovulation, TRENDS IN ENDOCRINOLOGY AND METABOLISM, 19巻, 6号, pp. 191-196, 2008
  57. Sequential exposure of porcine cumulus cells to FSH and LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, BIOLOGY OF REPRODUCTION, pp. 150-150, 2008
  58. PGE2 and progesterone enhance EGFR activation in porcine cumulus cells via the induction of TACE/ADAM17 expression but not EGF like factors expression during oocyte maturation in vivo and in vitro, BIOLOGY OF REPRODUCTION, pp. 150-150, 2008
  59. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization, DEVELOPMENT, 135巻, 11号, pp. 2001-2011, 2008
  60. The involvement of the Toll-like receptor family in ovulation, JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 25巻, 6号, pp. 223-228, 2008
  61. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, REPRODUCTION, 136巻, 1号, pp. 9-21, 2008
  62. Selective expression of constitutively active KrasG12D in granulosa cells of the mouse ovary causes defects in follicle development and ovulation, BIOLOGY OF REPRODUCTION, pp. 127-127, 2008
  63. Assessment of human oocyte quality by cumulus cell morphology and circulating hormone profile, Reproductive BioMedicine Online, 14巻, 1号, 20070101
  64. Synaptosomal associated protein 25 (Snap25) gene expression is hormonally regulated during ovulation and is involved in cytokine/chemokine exocytosis from granulosa cells., Molecular Endocrinology, 21巻, 10号, pp. 2487-2502, 20071001
  65. Involvment of Ca2+-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of matured rat oocytes., Theriogenology, 67巻, 3号, pp. 475-485, 20070301
  66. Hormone-induced expression of tumor necrosis factor alpha-converting enzyme/a disintegrin and metalloprotease-17 impacts porcine cumulus cell oocyte complex expansion and meiotic maturation via ligand activation of the epidermal growth factor receptor, ENDOCRINOLOGY, 148巻, 12号, pp. 6164-6175, 2007
  67. Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal that cumulus cells exhibit a diverse array of neuronal and immune-like functional activities: Are these cells multi-potential?, Molecular Endocrinology, 20巻, 6号, pp. in press, 20060601
  68. Paracrine and autocrine regulation of EGF-like factors in cumulus oocyte complexes (COCs) and granulosa cells: key roles for prostanglandin synthase 2 (Ptgs2) and progesterone receptor (Pgr), Molecular Endocrinology, 20巻, 6号, pp. in press, 20060601
  69. Induced expression of pattern recognition receptors (PRRs) in cumulus oocyte complexes (COCs): Novel evidence for immune-like functions during ovulation., Molecular Endocrinology, 20巻, 6号, pp. 3228-3239, 20060601
  70. Gonadotropin-induced delta 14-reductase and delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, Endocrinology, 146巻, pp. 186-194, 20050101
  71. The timing of MAP kinase inactivation effects on emission of polar body in porcine oocytes activated by Ca2+ ionophore, Molecular Reproduction and Development, 70巻, pp. 64-69, 20050201
  72. Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle., Animal Reproduction Science, 87巻, pp. 1-10, 20050601
  73. Contribution of high p34cdc2 kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes., Reproduction, 129巻, pp. 172-180, 20050401
  74. Transcripts encoding the enzymes that convert acetyl-CoA to cholesterol are induced in cumulus cells and are essential for progesterone biosynthesis and meiotic resumption of porcine oocytes., Japanese Journal of Reproductive Endocrinology, 10巻, pp. 15-20, 20051101
  75. TAF4b, a TBP Associated Factor is Required for Multiple Aspects of Oocyte Development., 発生生物学, 208巻, 6号, pp. 405-409, 20050615
  76. Mice null for Frizzled4 (Fzd4-/-) are infertile and exhibit impaired corpus lutea formation and function: a critical for vascularization., Biology of Reproduction, 73巻, 7号, pp. 1135-1146, 20050729
  77. Mitogen-Activated Protein Kinase Kinase Inhibitor Suppresses Cyclin B1 Synthesis and Reactivation of p34cdc2 Kinase, Which Improves Pronuclear Formation Rate in Matured Porcine Oocytes Activated by Ca2+ Ionophore, Biology of Reproduction, 70巻, pp. 797-804, 20040301
  78. Dramatic changese in motility and penetration competence of miniture pig spermatozoa frozen after exposure to seminal plasma, Theriogenology, 61巻, 42769号, pp. 351-364, 20040101
  79. Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes, Journal of Molecular Endocrinology, pp. in press, 20040401
  80. The role of Calcium/Calmodulin-dependent protein kinase II (CaMKII) on inactivation of MAP kinase and p34cdc2 kinase during fertilization and activation in pig oocytes, Reproduction, 128巻, pp. 409-415, 20040401
  81. Down-regulated expression of ADAMTS-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes, Endocrinology, 145巻, pp. 4603-4614, 20040401
  82. Effects of adding LH to FSH-containing medium on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes, Animal Science Journal, pp. in press, 20040401
  83. FSH-induced activation of PI 3-kinase-PKB pathway is essential for LH receptor formation in cumulus cells during meiotic resumption of porcine oocytes., Japanese Journal of Reproductive Endocrinology, pp. in press, 20040401
  84. Effects of adding luteinizing hormone to a medium containing follicle stimulating hormone on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes, Animal Science Journal, 75巻, pp. 515-523, 20040401
  85. The production of progesterone from de novo-synthesized cholesterol in cumulus cells, and its physiological role during meiotic resumption of porcine oocytes, Biology of Reproduction, 68巻, 4号, pp. 1193-1198, 20030401
  86. Effects of estrone sulfate injection on reproductive functions in male Japanese quail., Journal of Poultry Science, 40巻, pp. 243-247, 20031001
  87. PI 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes., Journal of Endocrinology, 179巻, pp. 25-34, 20031001
  88. Effect of Protein kinase C activator on mitogen-activated protein kinase and p34cdc2 kinase activity during parthenogenetic activation of porcine oocytes by calcium ionophore., Biology of Reproduction, 69巻, pp. 1675-1682, 20031101
  89. LH receptor formation in cumulus cells surrounding porcine oocytes, and its role during meiotic maturation of porcine oocytes., Biology of Reproduction, 68巻, 4号, pp. 1142-1149, 20030401
  90. Time dependent changes in progesterone receptor expression in cumulus cells during meiotic resumption of porcine oocytes, Journal of Mammalian Ova Research, 20巻, pp. 113-117, 20031001
  91. Time dependent changes in progesterone receptor expression in cumulus cells during meiotic resumption of porcine oocytes, Journal of Mammalian Ova Research, 20巻, pp. 113-117, 20030401
  92. LH reduces proliferative activity of cumulus cells and accelerates GVBD of porcine oocytes, Molecular and Cellular Endocrinology, 209巻, pp. 43-50, 20030401
  93. Roles of cAMP in Regulation of both MAP kinase and p34cdc2 kinase activity during Meiotic Progression,especially beyond the MI stage., Molecular Reproduction and Development, 62巻, 1号, pp. 124-131, 20020501
  94. ★, FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes., Molecular Human Reproduction, 8巻, pp. 621-628, 20020701
  95. Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media., Reproduction, 124巻, pp. 557-564, 20021001
  96. Both Ca2+-PKC pathway and cAMP-PKA pathway require for progesterone production in FSH- and LH-stimulated cumulus cells during in vitro maturation of porcine oocytes., J Mamm Ova Res, 19巻, 3号, pp. 81-88, 20021001
  97. Dynamic changes of connexin-43, gap junctional protein, in outer layers of cumulus cells are regulated by PKC and PI 3-kinase during meiotic resumption in porcine oocytes, Biology of Reproduction, 64巻, 4号, pp. 1255-1263, 20010401
  98. PI 3-kinase in cumulus cells and oocytes is responsible for activation of oocyte MAP kinase during meiotic progression beyond the MI stage in pigs, Biology of Reproduction, 64巻, 4号, pp. 1106-1114, 20010401
  99. The drop of cAMP level due to the closure of gap junctional communication between cumulus cells and oocyte is essential for meiotic progression beyond the MI stage in porcine oocytes, J Mamm Ova Res, 18巻, 3号, pp. 99-105, 20010401
  100. Progression of nuclear maturation and p34cdc2 kinase activity in porcine oocytes during in vitro culture in different medium, J Mamm Ova Res, 18巻, 1号, pp. 39-43, 20010401
  101. Survival of Boar Spermatozoa Frozen in Diluents of Varying Osmolality., Theriogenology, 56巻, 8号, pp. 447-458, 20010401
  102. ★, Inhibition of PI 3-kinase or MEK Leads to Suppression of p34cdc2 kinase Activity and Meiotic Progression beyond the MI stage in Porcine Oocyte Surrounded with Cumulus Cells., Biology of Reproduction, 65巻, 2号, pp. 442-448, 20010401
  103. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, blocks in vitro fertilization and embryonic development of bovine oocytes matured in vitro with epidermal growth factor., J Mamm Ova Res, 17巻, 3号, pp. 109-114, 20000401
  104. Meiotic Maturation of Bovine Oocytes Cultured In vitro with EGF and Wortmannin., J Mamm Ova Res, 17巻, 3号, pp. 103-108, 20000401
  105. Activation by stimuli of sperm penetration in bovine oocytes treated with high concentration of BAPTA/AM., J Mamm Ova Res, 17巻, 3号, pp. 96-102, 20000401
  106. Effects of wortmannin on the kinetics of GVBD and the activities of the maturation-promoting factor and mitogen-activated protein kinase during bovine oocyte maturation in vitro, Theriogenology, 53巻, 5号, pp. 1797-1806, 20000401
  107. Phosphorylation of Connexin-43, Gap Junctional Protein, in Cumulus Cells Are Regulated by Mitogen Activated Protein Kinase and Phosphatidylinositol 3-Kinase during In Vitro Meiotic Resumption in Porcine Follicular Oocytes., J Mamm Ova Res, 16巻, 3号, pp. 37-42, 19990401
  108. A Role for PI 3-Kinase in bovine oocytes nuclear maturation., Theriogenology, 50巻, 7号, pp. 347-356, 19980401
  109. Phosphatidylinositol 3-Kinase in Cumulus Cells is Responsible for Meiotic Progression from MⅠ to MⅡ Stage in Porcine Follicular Oocytes., J Mamm Ova Res, 15巻, 3号, pp. 68-76, 19980401
  110. ★, Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002 on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes., J Reprod Dev, 44巻, 5号, pp. 281-288, 19980401
  111. Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs, JOURNAL OF OVARIAN RESEARCH, 2巻, 1号, 2009
  112. Fecal progestagen and estrone during pregnancy in a giraffe: A case report, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53巻, 1号, pp. 159-164, 2007
  113. MAP kinase およびPl 3-kinaseが卵丘細胞間ギャップ結合の構成因子Connexin-43のリン酸化をブタ卵胞卵子の減数分裂再開時に制御する, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 16巻, 1号, pp. 37-42, 19990401
  114. ERK1^<-/-> ; ERK2^ ; Cyp19A1Cre mice を用いた卵胞内エストロジェン濃度低下と排卵機構の解析, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 26巻, 2号, 20090401
  115. ラット排卵卵子の減数分裂停止に関わるMos/MEK/MAPK系と p34^ kinase の相補的な機能, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 22巻, 2号, 20050401
  116. 卵丘細胞のPI 3-kinase とMI以降のブタ卵子のp34^ kinase との関係, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 16巻, 2号, 19990401
  117. 異なる成熟培地で培養したブタ卵子のp34cdc2 kinase活性の変化と核相の進行, Journal of mammalian ova research = 日本哺乳動物卵子学会誌, 18巻, 1号, pp. 39-43, 20010401

招待講演、口頭・ポスター発表等

  1. 顆粒膜細胞が分泌する局所因子による卵丘細胞の機能的変化と卵成熟制御, 島田昌之, 日本生殖医学会学術総会, 2015年, 招待, 日本語, 日本生殖医学会, 横浜市
  2. 加齢に伴う卵巣機能低下の卵成熟への影響―分子内分泌学的解析―, 島田昌之, 日本受精着床学会学術集会, 2015年, 招待, 日本語, 日本受精着床学会, 東京都
  3. 受精に関わる免疫機能の解明とそれを利用した繁殖技術の開発, 島田昌之, 日本生殖工学会学術集会, 2015年, 招待, 日本語, 日本生殖工学会, 東京都
  4. 採卵された(てしまった)未成熟卵を如何に培養するか?, 島田昌之, 日本受精着床学会, 2016年07月23日, 招待, 日本語, 長野県
  5. 排卵過程における卵丘細胞の機能性変化とそれに基づいたIVM法の開発, 島田 昌之, 日本生殖医療エンジニアリング研究会 第二回学術集会, 2007年02月, 招待, 日本語
  6. Cumulus cells are essential mediators of LH induced ovulation stimuli from granulosa cells to the oocyte., 島田昌之, 2014年08月, 招待
  7. 遺伝子改変(欠損)マウスの解析から,体外成熟培養,体外受精を考える, 島田昌之, 日本卵子学会, 2014年05月, 招待
  8. Nrg1遺伝子欠損マウスから学ぶ,ARTにおける受精不成功の原因追及とその解決法, 島田昌之, 勝川ART研究会, 2014年05月, 招待
  9. 卵胞発育におけるエピジェニック制御, 島田昌之, 生殖バイオロジー東京シンポ, 2014年07月, 招待
  10. 精子の運動性制御機構の解析とそれを基にした精子凍結/液状保存法の開発, 島田昌之, ブタ繁殖衛生セミナー, 2014年11月, 招待
  11. 2015年, 招待, 英語
  12. 未成熟卵の培養 ~卵成熟メカニズムと培養環境~, 島田昌之, 日本A-PART学術講演会, 2017年03月19日, 招待, 日本語
  13. 加齢に伴う卵胞発育不全(ローレスポンダー)の原因探索とその治療戦略, 島田昌之, 田中温, 第27回臨床内分泌代謝Update 「性腺」, 2017年11月23日, 招待, 日本語
  14. 多嚢胞性卵巣症候群における内分泌破綻-マウスモデルを用いた原因と卵胞発達・排卵への影響探索-, 島田昌之, 第69回日本産科婦人科学会学術講演会, 2017年04月14日, 招待, 日本語
  15. 卵巣の老化と卵成熟障害, 島田昌之, 第35回日本受精着床学会, 2017年07月06日, 招待, 日本語
  16. 高FSH/高LHがもたらす卵巣皮質の繊維化による2次卵胞発育不全 ~Nrg1KOマウスをモデルとした卵巣加齢化研究~, 島田昌之, 第62回日本生殖医学会学術講演会・総会, 2017年11月16日, 招待, 日本語
  17. The aging ovary: restriction of follicle growth by abnormal endocrine functions and ovarian stromal matrix fibrosis, 島田昌之, 15th International Symposium of Immunology of Reproduction, 2018年06月14日, 招待, 英語
  18. The Aging Ovary: Abnormal Endocrine Functions Is there a role for Androgens, AR and Theca Cell Dysfunction?, 2018年09月10日, 招待, 英語
  19. 精液成分が決定する精子の直進運動性 ~その持続時間に関するミトコンドリア内セントラルドグマと酸化ストレスとの関係~, 島田昌之, 第18回 日本Men’s Health医学会, 2018年07月15日, 招待, 日本語
  20. 卵胞発育ステージに合わせたIVM, 島田昌之, 第36回 日本受精着床学会総会・学術集会, 2018年07月26日, 招待, 日本語
  21. 加齢に伴う卵巣機能低下の原因探索, 島田昌之, 第36回 日本受精着床学会総会・学術集会, 2018年07月26日, 招待, 日本語
  22. 卵胞発育過程における大規模なDNA脱メチル化による排卵準備, 島田昌之, 加齢に伴う卵巣機能低下の原因探索, 2018年07月26日, 招待, 日本語
  23. ブタ人工授精技術のイノベーション, 島田昌之, 第111回日本繁殖生物学会, 2018年09月13日, 招待, 日本語
  24. Neuregulin1による雌雄配偶子形成メカニズム, 島田昌之, 第91回日本内分泌学会学術総会, 2018年04月19日, 招待, 日本語
  25. 比較生殖生物学,精子の動物種間差 ~基礎研究を日々の臨床につなげるために~, 島田昌之, 第24回 日本臨床エンブリオロジスト学会, 2019年01月13日, 招待, 日本語

取得

  1. 特許権, 特許4783883, 2011年07月22日, 受胎率および産子数向上凍結精子およびその製法
  2. 特許権, 特許5422848, 2013年12月06日, 受胎率および産子数向上凍結精子およびその製法
  3. 特許権, KR:10-1457143, 2014年10月27日, 精子用希釈液、及び、これを用いた人工授精方法
  4. ZL201080026618.5, 2015年01月07日, 精子用希釈液、及び、これを用いた人工授精方法
  5. 2765855, 2015年02月03日, 精子用希釈液、及び、これを用いた人工授精方法
  6. 5733829, 2015年04月24日, 凍結された精子用希釈液、及び、これを用いた人工授精方法
  7. 5904369, 2016年03月25日, 精子保存液、精子保存方法及び人工授精方法
  8. 特許権, 5920787, 2016年/4月/2日, ヒトの体外受精用精液又は人工授精用精液の調整方法

外部資金

競争的資金等の採択状況

  1. 農林水産業・食品産業 科学技術研究推進事業, 卵受精保持能,子宮・卵管内精子運動調節機構に着眼した効率的ブタ人工授精法の開発, 2015年04月09日, 2016年03月22日
  2. 民間からの助成金, 卵管内における精子,卵子,初期胚,卵丘細胞,卵管上皮細胞のネットワーク, 2007年
  3. ブタ凍結精液の受託生産を目指した精液輸送液,人工精漿の開発, 2010年
  4. 科学研究費助成事業(新学術領域研究(研究領域提案型)), 卵巣および精巣局所因子の発現,機能解析による配偶子形成機構の解明, 2013年, 2014年
  5. 科学研究費助成事業(若手研究(A)), 早期生殖能力減退マウスの分子内分泌学的解析と家畜育種マーカーへの応用, 2012年, 2014年
  6. 科学研究費助成事業(特定領域研究), 卵丘細胞のERKとPKB系に着目した哺乳動物卵子減数分裂再開制御因子の探索, 2009年, 2010年
  7. 科学研究費助成事業(基盤研究(A)), 卵子の微小器官動態制御因子の同定による次世代型IVMFC技術開発, 2009年, 2011年
  8. 科学研究費助成事業(若手研究(A)), マイクロRNAの比較発現解析による排卵機構の解明,その卵子成熟培養系への応用, 2009年, 2011年
  9. 科学研究費助成事業(若手研究(A)), 網羅的遺伝子発現解析による新たな哺乳動物卵子体外成熟培養系の開発, 2006年, 2008年
  10. 科学研究費助成事業(若手研究(B)), 機能的LH受容体の発現誘導によるブタ体外成熟卵子の発生能向上に関する研究, 2004年, 2005年
  11. 科学研究費助成事業(基盤研究(B)), 性ステロイドによる環境汚染と動物・植物への影響評価, 2003年, 2004年
  12. 科学研究費助成事業(基盤研究(C)), クロダイ放流が天然資源増大に及ぼす実証遺伝学的研究, 2002年, 2005年
  13. 科学研究費助成事業(若手研究(B)), LH受容体発現解析によるブタ体外成熟卵子の発生能向上に関する生理・生化学的研究, 2002年, 2003年
  14. 科学研究費助成事業(萌芽的研究), 暑熱の影響を受けた乳腺細胞のホルモン感受性の解析, 2001年, 2002年
  15. 科学研究費助成事業(基盤研究(C)), ブタ精子の耐凍能に関する生理・生化学的研究, 2000年, 2002年
  16. 農林水産業・食品産業 科学技術研究推進事業, 卵受精保持能,子宮・卵管内精子運動調節機構に着眼した効率的ブタ人工授精法の開発, 2014年07月02日, 2015年03月23日
  17. 科学研究費助成事業(基盤研究(B)), ノンコーディングRNAに着眼した家畜卵胞発育のエピゲノム制御機構の解明, 2016年, 2018年
  18. 成育疾患克服等総合研究事業, ①卵胞を構成する体細胞を用いた卵子加齢変化の診断法の開発、②卵子の質の低下による高齢不妊の治療法の開発、③卵巣組織凍結法の改良、有効性・安全性の検証, 2016年04月01日, 2017年03月31日
  19. 農林水産業・食品産業 科学技術研究推進事業, 卵受精保持能,子宮・卵管内精子運動調節機構に着眼した効率的ブタ人工授精法の開発, 2016年04月01日, 2017年03月31日
  20. 科学研究費助成事業(挑戦的研究(萌芽)), モデルマウス作製による受精障害予防と治療法開発への挑戦, 2017年, 2018年
  21. 成育疾患克服等総合研究事業, ①卵胞を構成する体細胞を用いた卵子加齢変化の診断法の開発、②卵子の質の低下による高齢不妊の治療法の開発、③卵巣組織凍結法の改良、有効性・安全性の検証, 2017年04月01日, 2018年03月31日
  22. 成育疾患克服等総合研究事業, ①卵胞を構成する体細胞を用いた卵子加齢変化の診断法の開発、②卵子の質の低下による高齢不妊の治療法の開発、③卵巣組織凍結法の改良、有効性・安全性の検証, 2018年04月01日, 2019年03月31日
  23. 科学研究費助成事業(基盤研究(B)), 卵巣間質の間葉系幹細胞に着眼した卵巣加齢化メカニズム解明と予防・根治法開発, 2019年, 2021年

社会活動

その他社会貢献活動(広大・部局主催含)

  1. 愛知県臨床獣医師の会, 光と繁殖の生理から, 繁殖技術の最新知見まで ~LED照明による排卵同期化,卵巣機能亢進,繁殖成績向上~, 2019年/07月/10日, 講師, 講演会, 社会人・一般