片柳 克夫Katsuo Katayanagi

Last Updated :2024/05/07

所属・職名
大学院統合生命科学研究科 准教授
メールアドレス
kkatahiroshima-u.ac.jp
自己紹介
蛋白質や核酸などの生体高分子のX線結晶構造解析による分子構造の解明に取り組んできた。それら蛋白質の由来はヒト、黄色ブドウ球菌、植物、海藻など多様で、さらに立体構造からみた分子進化にも興味を持っている。

基本情報

主な職歴

  • 1986年04月01日, 1993年12月31日, 蛋白工学研究所
  • 1994年01月01日, 1996年09月30日, 三菱化学
  • 1996年10月01日, 2019年03月31日, 広島大学, 大学院理学研究科, 助教授
  • 2019年04月01日, 広島大学, 大学院統合生命科学研究科, 准教授

学位

  • 博士(薬学) (大阪大学)
  • 工学修士 (東京工業大学)

研究分野

  • 生物学 / 生物科学 / 構造生物化学

研究キーワード

  • 構造機能相関
  • 蛋白質結晶学

教育活動

授業担当

  1. 2024年, 学部専門, セメスター(前期), 卒業研究
  2. 2024年, 学部専門, セメスター(後期), 卒業研究
  3. 2024年, 学部専門, セメスター(前期), 化学実験I
  4. 2024年, 学部専門, セメスター(後期), 化学実験II
  5. 2024年, 学部専門, 3ターム, 生物構造化学
  6. 2024年, 修士課程・博士課程前期, 1ターム, 統合生命科学特別講義
  7. 2024年, 修士課程・博士課程前期, 1ターム, 生命理学概論
  8. 2024年, 修士課程・博士課程前期, 年度, 数理生命科学特別研究
  9. 2024年, 修士課程・博士課程前期, 1ターム, 分子生物物理学
  10. 2024年, 修士課程・博士課程前期, 2ターム, プロテオミクス
  11. 2024年, 修士課程・博士課程前期, 2ターム, プロテオミクス実験法・同実習
  12. 2024年, 修士課程・博士課程前期, 2ターム, 生命理学特論C
  13. 2024年, 修士課程・博士課程前期, 4ターム, 生命理学特論D
  14. 2024年, 修士課程・博士課程前期, セメスター(前期), 生命理学特別演習A
  15. 2024年, 修士課程・博士課程前期, セメスター(後期), 生命理学特別演習B
  16. 2024年, 修士課程・博士課程前期, 2ターム, 放射光科学特論B

研究活動

学術論文(★は代表的な論文)

  1. ★, Three-dimensional structure of ribonuclease H from E. coli., NATURE, 347巻, 6290号, pp. 306-309, 19900920
  2. Stabilization of Escherichia coli ribonuclease H by introduction of an artificial disulfide bond., JOURNAL OF BIOLOGICAL CHEMISTRY, 19巻, 8号, pp. 6038-6044, 199104
  3. Structural models of ribonuclease H domains in reverse transcriptases from retroviruses., NUCLEIC ACIDS RESEARCH, 19巻, 8号, pp. 1817-1823, 199104
  4. Effect of mutagenesis at each of five histidine residues on enzymic activity and stability of ribonuclease H from Escherichia coli, EUROPEAN JOURNAL OF BIOCHEMISTRY, 198巻, 2号, pp. 437-440, 199106
  5. Atomic structure of a pyrimidine-dimer specific exision-repair enzyme from bacterio-phage T4., NUCLEIC ACIDS RESEARCH SYMPOSIUM, Series 24巻, pp. 181-184, 199109
  6. Cocrystallization of Escherichia coli RNase H with synthetic DNA/RNA hybrid oligomers., NUCLEIC ACIDS RESEARCH SYMPOSIUM, Series 24巻, pp. 253, 199109
  7. How does RNase H recognize a DNA-RNA hybrid ?, PROCEEDINGS OF NATIONAL ACADEMY OF SCIENCE U.S.A., 88巻, 24号, pp. 11535-11539, 199112
  8. ★, Structural details of ribonuclease H from Escherichia coli as refined to an atomic resolution., JOURNAL OF MOLECULAR BIOLOGY, 223巻, 4号, pp. 1029-1052, 199202
  9. ★, X-ray structure of T4 endonuclease V; An excision repair enzyme specific for a pyrimidine dimer., SCIENCE, 256巻, 5056号, pp. 523-526, 199204
  10. Effect of cavity-modulating mutations on the stability of Escherichia coli ribonuclease HI., EUROPEAN JOURNAL OF BIOCHEMISTRY, 206巻, 2号, pp. 337-343, 199206
  11. Crystal structure of ribonuclease H from Thermus thermophilus HB8 refined at 2.8 A resolution., JOURNAL OF MOLECULAR BIOLOGY, 230巻, 2号, pp. 529-542, 199303
  12. The crystal structure of ribonuclease F1 of Fusarium moniliforme in its free form and in complex with 2’GMP., JOURNAL OF MOLECULAR BIOLOGY, 230巻, 2号, pp. 979-996, 199304
  13. ★, Crystal structures of ribonuclease HI active site mutants from Escherichia coli., JOURNAL OF BIOLOGICAL CHEMISTRY, 268巻, 29号, pp. 22092-22099, 199312
  14. ★, Crystal structure of Escherichia coli RNase HI in complex with Mg2+ at 2.8 A resolution; Proof for a single Mg2+-binding site., PROTEINS; STRUCTURE, FUNCTIONS, AND GENETICS, 17巻, 4号, pp. 337-346, 199312
  15. Crystallization and preliminary crystallographic data of the alpha-amylase inhibitors, Haim I and Paim I., JOURNAL OF BIOCHEMISTRY, 115巻, 1号, pp. 168-170, 199401
  16. Crystal structure of T4 endonuclease V. An excision repair enzyme for a pyrimidine dimer., ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 726巻, pp. 198-207, 199404
  17. ★, Architectures of class-defining and specific domains of glutamyl-tRNA synthetase., SCIENCE, 267巻, 5206号, pp. 1958-1965, 199505
  18. Crystal structure of a pyrimidine dimer-specific excision repair enzyme from bacteriophage T4; refinement at 1.45 A and X-ray analysis of the three active site mutants., JOURNAL OF MOLECULAR BIOLOGY, 249巻, 2号, pp. 360-375, 199506
  19. Proposal of new roles for two invariant residues in Escherichia coli ribonuclease H., PROTEIN ENGINEERING, 9巻, 10号, pp. 857-867, 19961001
  20. The translin ring specifically recognizes DNA ends at recombination hot spots in the human genome., JOURNAL OF BIOLOGICAL CHEMISTRY, 272巻, 17号, pp. 11402-11407, 19970425
  21. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 128巻, 1号, pp. 21-27, 20000701
  22. Crystal structure analysis of new-type lectin from algae., Spring-8 User Experimental Report, 7(2001A)巻, pp. 274, 200110
  23. Effects of mutation and ligand binding on the compressibility of protein, PROGRESS IN BIOCHEMISTRY, 19巻, pp. 63-70, 200204
  24. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, NUCLEIC ACIDS RESEARCH, 32巻, 17号, pp. 5291-5302, 2004
  25. Evolutional design of a hyperactive cysteine- and methionine-free mutant of Escherichia coli dihydrofolate reductase, JOURNAL OF BIOLOGICAL CHEMISTRY, 281巻, 19号, pp. 13234-13246, 20060512
  26. Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans, INFECTION AND IMMUNITY, 74巻, 12号, pp. 7014-7020, 200612
  27. Fluorescent probes for the analysis of DNA strand scission in base excision repair, NUCLEIC ACIDS RESEARCH, 38巻, 7号, 201004
  28. The initial N-O bond cleavage mechanism of nitrite in assimilatory- nitrite-reductase from tabacum: effective utilization of X-ray structures and UV-Vis microspectroscopy., JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY SUPPLEMENT, 16巻, pp. S55, 201111
  29. ★, Structure-function relationship of assimilatory nitrite reductases from the leaf and root of tobacco based on high-resolution structures, PROTEIN SCIENCE, 21巻, 3号, pp. 383-395, 201203
  30. Structural implication for the impaired binding of W150A mutant LOX-1 to oxidized low density lipoprotein, OxLDL, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 5号, pp. 739-749, 201205
  31. ★, The reductive reaction mechanism of tobacco nitrite reductase derived from a combination of crystal structures and ultraviolet-visible microspectroscopy, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 80巻, 8号, pp. 2035-2045, 201208
  32. ★, X-Ray Crystal Structure of a Mutant Assimilatory Nitrite Reductase That Shows Sulfite Reductase-Like Activity, CHEMISTRY & BIODIVERSITY, 9巻, 9号, pp. 1989-1999, 201209
  33. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 201503
  34. Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119巻, 41号, pp. 13002-13008, 20151015
  35. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1, EXTREMOPHILES, 21巻, 3号, pp. 591-602, 201705
  36. Negative Cooperativity in Guest Binding of a Ditopic Self-Folding Biscavitand, Organic Letters, 23巻, pp. 6217-6221, 20210707
  37. Pivotal role of a conserved histidine in Escherichia coli ribonuclease HI as proposed by X-ray crystallography, Acta Cryst. D, 78巻, pp. 390-398, 20220124

著書等出版物

  1. 1992年02月, RNase H; three-dimensional structure and function. , Bulletin of Institute Pasteur 90, 1992年, FEB, 単行本(学術書), 森川 耿右, 片柳 克夫, pp.71-82
  2. 1993年, 新生化学実験講座 第7章 X線, 東京化学同人, 1993年, 単行本(学術書), 片柳 克夫 森川耿右 , pp.131-168
  3. 1993年, RNase H; crystallographic approach to function, in Molecular Biology (Life Science Advances) 13, 1993年, 単行本(学術書), 片柳克夫, 森川耿右, pp.193-202
  4. 1993年01月, タンパク質工学とX線解析-RNase H の構造機能相関を例として-. , 細胞工学12, 秀潤社, 1993年01月, 単行本(学術書), 片柳克夫, 森川耿右, 11-20
  5. 1998年, Crystal structures of ribonuclease H of prokaryotes. , in "Ribonuclease H" (ed. R.J. Crouch), Inserm, 1998年, 単行本(学術書), 森川 耿右, 片柳克夫, pp.181-193

招待講演、口頭・ポスター発表等

  1. Structure and function of assimilatory nitrite reductases from tobacco leaf and root., Katsuo Katayanagi, Shogo Nakano, WGC-2015, 2015年11月13日, 招待, 英語, Qingtao (PRC)