坂本 尚昭Naoaki Sakamoto

Last Updated :2017/09/01

所属・職名
大学院理学研究科 生命理学講座 准教授
メールアドレス
naosakahiroshima-u.ac.jp

基本情報

学位

  • 博士(理学) (広島大学)
  • 修士(理学) (広島大学)

研究分野

  • 総合生物 / ゲノム科学 / ゲノム生物学

研究キーワード

  • ウニ
  • DNA構造
  • クロマチン
  • インスレーター

所属学会

教育活動

授業担当

  1. 2017年, 教養教育, セメスター(前期), 情報活用演習[1理生]
  2. 2017年, 教養教育, セメスター(前期), 教養ゼミ
  3. 2017年, 学部専門, セメスター(前期), 生物科学セミナー
  4. 2017年, 学部専門, セメスター(前期), 先端生物学
  5. 2017年, 学部専門, セメスター(前期), 分子遺伝学A
  6. 2017年, 学部専門, セメスター(後期), 分子遺伝学演習
  7. 2017年, 学部専門, セメスター(前期), 卒業研究
  8. 2017年, 学部専門, セメスター(後期), 卒業研究
  9. 2017年, 学部専門, セメスター(前期), 生物科学基礎実験I
  10. 2017年, 学部専門, セメスター(後期), 生物科学基礎実験II
  11. 2017年, 学部専門, セメスター(前期), 生物科学基礎実験III
  12. 2017年, 学部専門, セメスター(後期), 生物科学基礎実験IV
  13. 2017年, 学部専門, セメスター(前期), Recent Developments in Biological Sciences
  14. 2017年, 修士課程・博士課程前期, セメスター(前期), 生命理学概論
  15. 2017年, 修士課程・博士課程前期, セメスター(前期), 数理分子生命理学セミナー
  16. 2017年, 修士課程・博士課程前期, セメスター(後期), 数理分子生命理学セミナー
  17. 2017年, 修士課程・博士課程前期, セメスター(前期), 数理分子生命理学特別研究
  18. 2017年, 修士課程・博士課程前期, セメスター(後期), 数理分子生命理学特別研究
  19. 2017年, 修士課程・博士課程前期, セメスター(後期), ゲノミクス
  20. 2017年, 修士課程・博士課程前期, セメスター(前期), 分子遺伝学セミナー
  21. 2017年, 修士課程・博士課程前期, セメスター(後期), 分子遺伝学セミナー

研究活動

学術論文(★は代表的な論文)

  1. The Egg Nucleus Regulates the Behavior of Sperm Nuclei as well as Cycling of MPF in Physiologically Polyspermic Newt Eggs., Developmental Biology, 160巻, 1号, pp.15-pp.27
  2. Corrected Structure of the 5' Flanking Region of Arylsulfatase Gene of the Sea Urchin, Hemicentrotus pulcherrimus., Development Growth and Differentiation, 36巻, 4号, pp.633-pp.636
  3. ★, A Triplex DNA Structure of the Polypyrimidine: Polypurine Stretch in the 5' Flanking Region of the Sea Urchin Arylsulfatase Gene., Zoological Science, 13巻, 1号, pp.105-pp.109
  4. ★, Two Isoforms of Orthodenticle-Related Proteins (HpOtx) Bind to the Enhancer Element of Sea Urchin Arylsulfatase Gene., Developmental Biology, 181巻, 2号, pp.284-pp.295
  5. Structure and Function of Sea Urchin Orthodenticle-Related Gene (HpOtx)., Developmental Biology, 193巻, 2号, pp.139-pp.145
  6. Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNAs during early development., International Journal of Developmental Biology, 42巻, 5号, pp.645-pp.651
  7. ★, Self-Association Properties of Long GAA•TTC Repeats in R•R•Y Triplex Structures from Friedreich's Ataxia., Molecular Cell, 3巻, 3号, pp.465-pp.475
  8. A nonpathogenic GAAGGA repeat in the Friedreich gene: implications for pathogenesis., Neurology, 53巻, 8号, pp.1854-pp.1857
  9. Functional annotation of a full-length mouse cDNA collection., Nature, 409巻, 6821号, pp.685-pp.690
  10. ★, Sticky DNA, a self-associated complex formed at long GAA•TTC repeats in intron 1 of the frataxin gene, inhibits transcription., Journal of Biological Chemistry, 276巻, 29号, pp.27171-pp.27177
  11. ★, GGA•TCC-interrupted triplets in long GAA•TTC repeats inhibit the formation of triplex and sticky DNA structures, alleviate transcription inhibition, and reduce genetic instabilities., Journal of Biological Chemistry, 276巻, 29号, pp.27178-pp.27187
  12. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo., Development, 129巻, 22号, pp.5205-pp.5216
  13. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos., Development Genes and Evolution, 213巻, 1号, pp.44-pp.49
  14. Pro-apoptotic protein kinase C delta is associated with intranuclear inclusions in a transgenic model of Huntington's disease., Journal of Neurochemistry, 87巻, 2号, pp.395-pp.406
  15. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus., Development Growth and Differentiation, 46巻, 1号, pp.61-pp.67
  16. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos., Development Growth and Differentiation, 46巻, 4号, pp.335-pp.341
  17. Decreased expression of hypothalamic neuropeptides in Huntington disease transgenic mice with expanded polyglutamine-EGFP fluorescent aggregates., Journal of Neurochemistry, 93巻, 3号, pp.641-pp.653
  18. ★, Unichrom, a novel nuclear matrix protein, binds to the ars insulator and canonical Mars., Zoological Science, 23巻, 1号, pp.9-pp.21
  19. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos., Gene Expression Patterns, 6巻, 5号, pp.572-pp.577
  20. Sodium channel beta4 subunit: down-regulation and possible involvement in neuritic degeneration in Huntington's disease transgenic mice., Journal of Neurochemistry, 98巻, 2号, pp.518-pp.529
  21. Functional analysis of the sea urchin-derived arylsulfatase (Ars)-element in mammalian cells., Genes to Cells, 11巻, 9号, pp.1009-pp.1021
  22. Analysis of cis-regulatory elements controlling spatio-temporal expression of T-brain gene in sea urchin, Hemicentrotus pulcherrimus., Mechanisms of Development, 125巻, 1-2号, pp.2-pp.17
  23. The Ars insulator facilitates I-SceI meganuclease-mediated transgenesis in the sea urchin embryo., Developmental Dynamics, 237巻, 9号, pp.2475-pp.2482
  24. Role of nanos homolog during sea urchin development., Developmental Dynamics, 238巻, 10号, pp.2511-pp.2521
  25. Targeted mutagenesis in the sea urchin embryo using zinc-finger nucleases., Genes to Cells, 15巻, 8号, pp.875-pp.885
  26. HpSulf, a heparan sulfate 6-O-endosulfatase, is involved in the regulation of VEGF signaling during sea urchin development., Mechanisms of Development, 127巻, 3-4号, pp.235-pp.245
  27. Mammalian arlysulfatase A functions as a novel component of the extracellular matrix., Connective Tissue Research, 51巻, 5号, pp.388-pp.396
  28. Implication of HpEts in gene regulatory networks responsible for specification of sea urchin skeletogenic primary mesenchyme cells., Zoological Science, 27巻, 8号, pp.638-pp.646
  29. ★, Dicer is required for the normal development of sea urchin, Hemicentrotus pulcherrimus., Zoological Science, 27巻, 6号, pp.477-pp.486
  30. HpSumf1 is involved in the activation of sulafatases responsible for regulation of skeletogenesis during sea urchin development., Development Genes and Evolution, 221巻, 3号, pp.157-pp.166
  31. ★, Nucleosome exclusion from the interspecies conserved central AT-rich region of the Ars insulator., Journal of Biochemistry, 151巻, 1号, pp.75-pp.87
  32. Zinc-finger nuclease-mediated targeted insertion of reporter genes for quantitative imaging of gene expression in sea urchin embryos., Proc Natl Acad Sci U S A, 109巻, 27号, pp.10915-pp.10920
  33. Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity., Scientific Reports, 3巻, pp.3379
  34. The 3 ' UTR of nanos2 directs enrichment in the germ cell lineage of the sea urchin, Developmental Biology, 377巻, 1号, pp.275-pp.283
  35. Targeted mutagenesis in sea urchin embryos using TALENs., Development Growth and Differentiation, 56巻, 1号, pp.92-pp.97
  36. Microhomology-mediated end-joining-dependent integration of donor DNA in cells and animals using TALENs and CRISPR/Cas9., Nature Communications, 5巻, pp.5560
  37. Simple Elastic Network Models for Exhaustive Analysis of Long Double-Stranded DNA Dynamics with Sequence Geometry Dependence., PLoS One, 211巻, 4号, pp.775-pp.784
  38. Viable neuronopathic Gaucher disease model in Medaka (Oryzias latipes) displays axonal accumulation of alpha-synuclein., PLoS Genetics, 11巻, pp.e1005065
  39. LOC-Based High-Throughput Cell Morphology Analysis System., IEEE transactions on automation science and engineering, 12巻, 4号, pp.1346-pp.1356
  40. In vivo tracking of histone H3 lysine 9 acetylation in Xenopus laevis during tail regeneration., Genes to Cells
  41. ★, Cilia play a role in breaking left–right symmetry of the sea urchin embryo., Genes to Cells

著書等出版物

  1. 2006年04月, DNA Structure, Chromatin and Gene Expression, Chapter 14, "Involvement of nuclear matrix and chromatin loop formation in the function of insulators.", Transworld Research Network, 2006年, 04, 単行本(学術書), 共編著, Akasaka K. , 8178952289
  2. 2009年03月31日, Shall We 遺伝学, 講談社, 2009年, 3, 単行本(学術書), 単著, 日本語, 坂本 尚昭, 4061554514, 184

受賞

  1. 2013年09月11日, 日本生化学会JB論文賞, 公益社団法人日本生化学会会長