藤本 勝巳Katsumi Fujimoto

Last Updated :2021/11/01

所属・職名
大学院医系科学研究科(歯) 助教
メールアドレス
kfujimohiroshima-u.ac.jp

基本情報

学位

  • 博士(医学) (大阪大学)
  • 農学修士 (大阪府立大学)

研究分野

  • 医歯薬学 / 歯学 / 機能系基礎歯科学
  • 医歯薬学 / 歯学 / 歯科医用工学・再生歯学

研究キーワード

  • 転写因子
  • 分化
  • 概日リズム

教育活動

授業担当

  1. 2021年, 学部専門, 2ターム, 口腔生化学II
  2. 2021年, 教養教育, 1ターム, 教養ゼミ
  3. 2021年, 学部専門, 4ターム, 生命科学
  4. 2021年, 学部専門, 1ターム, 口腔生化学I
  5. 2021年, 学部専門, 3ターム, 口腔生化学実習
  6. 2021年, 学部専門, 3ターム, 医科歯科分子生物学
  7. 2021年, 学部専門, 1ターム, 基礎栄養生化学
  8. 2021年, 学部専門, セメスター(後期), 基礎オーラルサイエンス実習
  9. 2021年, 学部専門, 1ターム, 基礎栄養生化学

研究活動

学術論文(★は代表的な論文)

  1. Stimulation of chondrogenic differentiation by a novel transcription factor DEC1 or a cartilage-characteristic membrane protein (MTf), Tissue Enginearing for Therapeutic Use 5 (Ikada Y.= Ed.), 5巻, pp. 117-128, 20010401
  2. Presynaptic localization of histamine H3-receptors in rat brain., Biochem. Biophys. Res. Commun., 177巻, 3号, pp. 907-912, 19910601
  3. Molecular cloning of the human histamine H1 receptor gene., Biochem. Biophys. Res. Commun., 201巻, 2号, pp. 894-901, 19940601
  4. ヒトヒスタミンH1受容体遺伝子のクローニング, 大阪大学医学雑誌, 46巻, pp. 71-80, 19940401
  5. Molecular cloning of the guinea-pig histamine H1 receptor gene., J. Biochem., 114巻, 3号, pp. 408-414, 19930901
  6. Does the [3H]mepyramine binding site represent the histamine H1 receptor? Re-examination of the histamine H1 receptor with quinine., J. Pharmacd. Exp. Ther., 268巻, 2号, pp. 959-964, 19940201
  7. Expression and characterization of the bovine histamine H1 receptor in cDNA-transfected C6 astroglioma cells., J. Biochem., 115巻, 6号, pp. 1155-1161, 19940601
  8. Site-directed mutagenesis of the histamine H1 receptor. The roles of Aspartic acid107, Asparagine198 and Threonine194. (共著), Biochem. Biophys. Res. Commun., 203巻, 2号, pp. 1096-1101, 19940901
  9. ホルボールエステルおよび糖質コルチコイドによるヒトヒスタミンH1受容体遺伝子転写活性の調節, Therapeutic Research, 16巻, pp. 181-188, 19950401
  10. Identification of protein kinase C phosphorylation sites involved in phorbol ester-induced desensitization of the histamine H1 recepor., Molecular Pharmacology, 55巻, 4号, pp. 735-743, 19990401
  11. Genomic cloning of the rat histamine H1 receptor., Biochem. Biophys. Res. Commun., 190巻, 1号, pp. 294-301, 19930101
  12. RGD-CAP (βig-h3) enhances the spreading of chondrocytes and fibroblasts via integrin α1β1., Biochem. Biophys. Acta=, 1451巻, pp. 196-205., 19990401
  13. Enhancement of cell adhesion and spreading by a cartilage-specific noncollagenous protein, cartilage matrix protein (CMP/matrilin-1), via integrin α1β1., J. Biol. Chem., 274巻, pp. 11417-11423., 19990401
  14. ヒトヒスタミンH1受容体遺伝子のクローニングとホルボールエステルによるヒトH1受容体情報伝達の長期的および短期的調節, Therapeutic Research, 15巻, pp. 218-228, 19940401
  15. ヒトヒスタミンH1受容体のリガンド認識機構, Therapeutic Research, 17巻, pp. 147-151, 19960401
  16. ヒトヒスタミンH1受容体遺伝子プロモーター領域の解析, Therapeutic Research, 18巻, pp. 45-50, 19970401
  17. ヒスタミン H1 受容体の活性化機構, Therapeutic Research, 19巻, pp. 208-210, 19980401
  18. ヒスタミンH1受容体の蛋白キナーゼCによるリン酸化部位の同定と受容体脱感作, Therapeutic Research, 20巻, pp. 17-24, 19990401
  19. リコンビナントヒスタミン H1 受容体発現 CHO 細胞における H1 受容体の Internalization 発現機構, Therapeutic Research, 21巻, pp. 41-45, 20000401
  20. Proinflammatory cytokines regulate the gene expression of hyaluronic acid synthetase in cultured rabbit synovial membrane cells, Connevtive Tissue Research, 42巻, 3号, pp. 187-195, 20011001
  21. Clock gene expression in the submandibular glands., Journal of Dental Research, 84巻, 12号, pp. 1193-1197, 20050401
  22. Rhythmic Expression of DEC1 and DEC2 in Peripheral Tissues: DEC2 is a Potent Suppressor for Hepatic Cytochrome P450s Opposing DBP., Genes to Cells, 9巻, 4号, pp. 317-329, 20040401
  23. Molecular cloning and characterization of DEC2, a new member of basic helix-loop-helix proteins, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 280巻, 1号, pp. 164-171, 20010112
  24. Chondrocyte-derived ezrin-like domain containing protein (CDEP), a rho guanine nucleotide exchange factor, is inducible in chondrocytes by parathyroid hormone and cyclic AMP and has transforming activity in NIH3T3 Cells, OSTEOARTHRITIS AND CARTILAGE, 9巻, pp. S64-S68, 2001
  25. Induction of basic helix-loop-helix protein DEC1 (BHLHB2)/Stra13/Sharp2 in response to the cyclic adenosine monophosphate pathway, EUROPEAN JOURNAL OF CELL BIOLOGY, 80巻, 5号, pp. 329-334, 200105
  26. Molecular cloning of rabbit hyaluronic acid synthases and their expression patterns in synovial membrane and articular cartilage, BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION, 1520巻, 1号, pp. 71-78, 20010730
  27. RGD-CAP (beta ig-h3) exerts a negative regulatory function on mineralization in the human periodontal ligament, JOURNAL OF DENTAL RESEARCH, 81巻, 12号, pp. 822-825, 200212
  28. Dec1 and Dec2 are regulators of the mammalian molecular clock, NATURE, 419巻, 6909号, pp. 841-844, 20021024
  29. Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in vitro, JOURNAL OF BIOLOGICAL CHEMISTRY, 277巻, 50号, pp. 48579-48586, 20021213
  30. Basic helix-loop-helix protein DEC1 promotes chondrocyte differentiation at the early and terminal stages, JOURNAL OF BIOLOGICAL CHEMISTRY, 277巻, 51号, pp. 50112-50120, 20021220
  31. Role of putative phosphorylation sites in down-regulation of histamine H-1 receptors, INFLAMMATION RESEARCH, 52巻, pp. S43-S44, 200304
  32. Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A, JOURNAL OF CELL SCIENCE, 116巻, 10号, pp. 2029-2038, 20030515
  33. Identification of amino acid residues responsible for agonist-induced down-regulation of histamine H-1 receptors, JOURNAL OF PHARMACOLOGICAL SCIENCES, 94巻, 4号, pp. 410-419, 200404
  34. A novel autofeedback loop of Dec1 transcription involved in circadian rhythm regulation, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 313巻, 1号, pp. 117-124, 20040602
  35. Dec1 and Dec2 expression is disrupted in the suprachiasmatic nuclei of Clock mutant mice, JOURNAL OF BIOLOGICAL RHYTHMS, 19巻, 2号, pp. 126-134, 200404
  36. Phosphorylation of human histamine H-1 receptors and its role in agonist-induced receptor internalization and down-regulation, INFLAMMATION RESEARCH, 53巻, pp. S73-S74, 200403
  37. Expression of the gene for Dec2, a basic helix-loop-helix transcription factor, is regulated by a molecular clock system, BIOCHEMICAL JOURNAL, 382巻, pp. 43-50, 20040815
  38. Two threonine residues and two serine residues in the second and third intracellular loops are both involved in histamine H-1 receptor downregulation, FEBS LETTERS, 573巻, 1-3号, pp. 226-230, 20040827
  39. Functional analysis of the basic helix-loop-helix transcription factor DEC1 in circadian regulation - Interaction with BMAL1, EUROPEAN JOURNAL OF BIOCHEMISTRY, 271巻, 22号, pp. 4409-4419, 200411
  40. Histamine H-1 receptor-mediated histamine H-1 receptor gene expression, INFLAMMATION RESEARCH, 54巻, pp. S42-S43, 200504
  41. Association of expression of receptor for advanced glycation end products and invasive activity of oral squamous cell carcinoma, ONCOLOGY, 69巻, 3号, pp. 246-255, 2005
  42. Mechanical regulation of terminal chondrocyte differentiation via RGD-CAP/beta ig-h3 induced by TGF-beta, CONNECTIVE TISSUE RESEARCH, 46巻, 4-5号, pp. 227-234, 2005
  43. Effects of overexpression of basic helix-loop-helix transcription factor Dec1 on osteogenic and adipogenic differentiation of mesenchymal stem cells, EUROPEAN JOURNAL OF CELL BIOLOGY, 85巻, 5号, pp. 423-431, 200605
  44. (57)Arg in the bHLH transcription factor DEC2 is essential for the suppression of CLOCK/BMAL2-mediated transactivation, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 17巻, 6号, pp. 1053-1056, 200606
  45. Recent advances in molecular pharmacology of the histamine systems: Regulation of histamine H-1 receptor signaling by changing its expression level, JOURNAL OF PHARMACOLOGICAL SCIENCES, 101巻, 1号, pp. 3-6, 200605
  46. Effects of fasting and re-feeding on the expression of Dec1, Per1, and other clock-related genes, JOURNAL OF BIOCHEMISTRY, 140巻, 3号, pp. 401-408, 200609
  47. Stimulation of histamine H-1 receptor up-regulates histamine H-1 receptor itself through activation of receptor gene transcription, JOURNAL OF PHARMACOLOGICAL SCIENCES, 103巻, 4号, pp. 374-382, 200704
  48. Transcriptional repression by the basic helix-loop-helix protein Dec2: Multiple mechanisms through E-box elements, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 19巻, 6号, pp. 925-932, 200706
  49. Multiple mechanisms regulate circadian expression of the gene, for cholesterol 7 alpha-hydroxylase (Cyp7a), a key enzyme in hepatic bile acid biosynthesis, JOURNAL OF BIOLOGICAL RHYTHMS, 22巻, 4号, pp. 299-311, 200708
  50. Basic-helix-loop-helix (bHLH) transcription factor DEC2 negatively regulates vascular endothelial growth factor expression, GENES TO CELLS, 13巻, 2号, pp. 131-144, 200802
  51. Period is involved in the proliferation of human pancreatic MIA-PaCa2 cancer cells by TNF-alpha, BIOMEDICAL RESEARCH-TOKYO, 29巻, 2号, pp. 99-103, 200804
  52. DEC1 modulates the circadian phase of clock gene expression, MOLECULAR AND CELLULAR BIOLOGY, 28巻, 12号, pp. 4080-4092, 200806
  53. Liver X receptors (LXR alpha and LXR beta) are potent regulators for hepatic Dec1 expression, GENES TO CELLS, 14巻, 1号, pp. 29-40, 200901
  54. The Basic Helix-Loop-Helix Proteins Differentiated Embryo Chondrocyte (DEC) 1 and DEC2 Function as Corepressors of Retinoid X Receptors, MOLECULAR PHARMACOLOGY, 76巻, 6号, pp. 1360-1369, 200912
  55. Anti-apoptotic effect of the basic helix-loop-helix (bHLH) transcription factor DEC2 in human breast cancer cells, GENES TO CELLS, 15巻, 4号, pp. 315-325, 201004
  56. Basic helix-loop-helix transcription factors DEC1 and DEC2 regulate the paclitaxel-induced apoptotic pathway of MCF-7 human breast cancer cells, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 27巻, 4号, pp. 491-495, 201104
  57. Time-Dependent Interaction between Differentiated Embryo Chondrocyte-2 and CCAAT/Enhancer-Binding Protein alpha Underlies the Circadian Expression of CYP2D6 in Serum-Shocked HepG2 Cells, MOLECULAR PHARMACOLOGY, 81巻, 5号, pp. 739-747, 201205
  58. Basic helix-loop-helix transcription factor DEC1 negatively regulates cyclin D1, JOURNAL OF PATHOLOGY, 224巻, 3号, pp. 420-429, 201107
  59. Smad3 and Snail show circadian expression in human gingival fibroblasts, human mesenchymal stem cell, and in mouse liver, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 419巻, 2号, pp. 441-446, 20120309
  60. Identification of a new clock-related element EL-box involved in circadian regulation by BMAL1/CLOCK and HES1, GENE, 510巻, 2号, pp. 118-125, 20121201
  61. The basic helix-loop-helix transcription factor DEC2 inhibits TGF-beta-induced tumor progression in human pancreatic cancer BxPC-3 cells, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 30巻, 3号, pp. 495-501, 201209
  62. IL-1 beta-mediated up-regulation of DEC1 in human gingiva cells via the Akt pathway, JOURNAL OF CELLULAR BIOCHEMISTRY, 113巻, 10号, pp. 3246-3253, 201210
  63. The BHLH transcription factor DEC1 plays an important role in the epithelial-mesenchymal transition of pancreatic cancer, INTERNATIONAL JOURNAL OF ONCOLOGY, 41巻, 4号, pp. 1337-1346, 201210
  64. Different circadian expression of major matrix-related genes in various types of cartilage: modulation by light-dark conditions, JOURNAL OF BIOCHEMISTRY, 154巻, 4号, pp. 373-381, 201310
  65. A Novel Protein, CHRONO, Functions as a Core Component of the Mammalian Circadian Clock, PLOS BIOLOGY, 12巻, 4号, 201404
  66. DEC1/STRA13/SHARP2 and DEC2/SHARP1 Coordinate Physiological Processes, Including Circadian Rhythms in Response to Environmental Stimuli, BHLH TRANSCRIPTION FACTORS IN DEVELOPMENT AND DISEASE, 110巻, pp. 339-372, 2014
  67. Involvement of c-Myc in the proliferation of MCF-7 human breast cancer cells induced by bHLH transcription factor DEC2, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 35巻, 3号, pp. 815-820, 201503
  68. Basic helix-loop-helix transcription factor DEC1 regulates the cisplatin-induced apoptotic pathway of human esophageal cancer cells, BIOMEDICAL RESEARCH-TOKYO, 36巻, 2号, pp. 89-96, 201504
  69. Down-regulation of histamine H-1 receptors by beta(2)-adrenoceptor-mediated inhibition of H-1 receptor gene transcription, JOURNAL OF PHARMACY AND PHARMACOLOGY, 60巻, 6号, pp. 747-752, 2008
  70. Heterologous up-regulation of the histamine H-1 receptor by M-3 muscarinic receptor-mediated activation of H-1-receptor gene transcription, JOURNAL OF PHARMACY AND PHARMACOLOGY, 59巻, 6号, pp. 843-848, 2007
  71. Interleukin-4 up-regulates histamine H1 receptors by activation of H1 receptor gene transcription, NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 381巻, 4号, pp. 305-313, 2010
  72. Re-examination of [3H]mepyramine binding assay for histamine H1 receptor using Quinine, Biochemical and Biophysical Research Communications, 189巻, 1号, pp. 378-384, 199211
  73. Transcriptional regulation of histamine H-1 receptor gene in HeLa cells, JOURNAL OF PHARMACOLOGICAL SCIENCES, 106巻, pp. 103P-103P, 2008
  74. Poly(ADP) ribose polymerase-1 involves in PMA-induced transcriptional up-regulation of histamine H-1 receptor gene in HeLa cells, JOURNAL OF PHARMACOLOGICAL SCIENCES, 112巻, pp. 107P-107P, 2010
  75. Roles of Dec1 and Dec2 in the core loop of the circadian clock, and clock outputs to metabolism., Hirosaki medical journal, 61巻, 0号, pp. S34-S42, 2010
  76. Ku86 represses PMA-induced up-regulation of histamine H-1 receptor gene expression in HeLa cells, JOURNAL OF PHARMACOLOGICAL SCIENCES, 115巻, pp. 124P-124P, 2011
  77. 無血清で増幅した間葉系幹細胞と歯髄細胞, 広島歯科医学雑誌, 39巻, 1号, pp. 1-8, 201107
  78. 循環器疾患における時計遺伝子DEC1の意義, Heart View, 16巻, 8号, pp. 103-107, 201209
  79. Characteristic expression of MSX1, MSX2, TBX2 and ENTPD1 in dental pulp cells, BIOMEDICAL REPORTS, 3巻, 4号, pp. 566-572, 201507
  80. DEC2-E4BP4 heterodimer represses the transcriptional enhancer activity of the EE element in the Per2 promoter, FRONTIERS IN NEUROLOGY, 6巻, 20150723
  81. Rhythmic expression of DEC2 protein in vitro and in vivo, BIOMEDICAL REPORTS, 4巻, 6号, pp. 704-710, 201606
  82. DEC2 is a negative regulator for the proliferation and differentiation of chondrocyte lineage-committed mesenchymal stem cells, INTERNATIONAL JOURNAL OF MOLECULAR MEDICINE, 38巻, 3号, pp. 876-884, 201609
  83. Role of MSX1 in Osteogenic Differentiation of Human Dental Pulp Stem Cells, STEM CELLS INTERNATIONAL, 2016
  84. Characterization of human dental pulp cells grown in chemically defined serum-free medium, BIOMEDICAL REPORTS, 8巻, 4号, pp. 350-358, 201804
  85. Deficiency of the basic helix-loop-helix transcription factor DEC1 prevents obesity induced by a high-fat diet in mice, GENES TO CELLS, 23巻, 8号, pp. 658-669, 201808
  86. VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) EXPRESSION IS NEGATIVELY REGULATED BY BASIC-HELIX-LOOP-HELIX (BHLH) TRANSCRIPTION FACTOR DEC2., Hirosaki Medical Journal, 61巻, pp. S43-S52, 20100708
  87. Genetic Markers Can Predict Chondrogenic Differentiation Potential in Bone Marrow-Derived Mesenchymal Stromal Cells, STEM CELLS INTERNATIONAL, 2018
  88. Potential Marker Genes for Predicting Adipogenic Differentiation of Mesenchymal Stromal Cells, APPLIED SCIENCES-BASEL, 9巻, 14号, 20190702
  89. DEC1 regulates the rhythmic expression of PPAR gamma target genes involved in lipid metabolism in white adipose tissue, GENES TO CELLS, 25巻, 4号, pp. 232-241, 202004

著書等出版物

  1. 1992年, Molecular biology of histamine H1-receptors, Annals of psychiatry / anuario psiquiatrico., Basic and clinical neurosciences 3, Prous Science, 1992年, 10, 単行本(学術書), 共著, h.fukui m.yamashita k.sugama y.horio k.fujimoto h.mizuguchi s.ito
  2. 2001年, Studies of histamine H1 receptor down-regulation using mutant receptors lacking putative phosphrylation sites., Histamine Research in the New Millennium., Elsevier Science B. V., 2001年, 12, 単行本(学術書), 共著, m.ogawa s.horio h.fukui k.fujimoto

招待講演、口頭・ポスター発表等

  1. ホメオボックス型転写因子MSX1による歯髄幹細胞の象牙芽細胞/骨芽細胞分化制御, 五藤紀子、藤本勝巳、依田浩子、大島勇人、河本健、能城光秀、宿南知佐、香西克之、加藤幸夫, 五藤紀子、藤本勝巳、依田浩子、大島勇人、河本健、能城光秀、宿南知佐、香西克之、加藤幸夫, 第87回日本生化学会大会, 2014年10月18日, 通常, 日本語, 京都
  2. The improved performance of mesenchymal stem cell cultures by fibronectin and mixed self-assembled monolayers under serum-free conditions, Sainik Ronald, Masami Kanawa, Isao Hirata, Koichi Kato, Katsumi Fujimoto, Chisa Shukunami, Yukio Kato, 第98回広島大学歯学会例会, 2014年11月09日, 通常, 英語, 広島県歯科医師会, 広島市
  3. ホメオボックス型転写因子MSX1による歯髄幹細胞分化制御, 藤本勝巳、五藤紀子、河本健、能城光秀、宿南知佐、香西克之、加藤幸夫, 第99回広島大学歯学会 併催 第54回広島県歯科医学会, 2015年11月08日, 通常, 日本語
  4. 歯髄幹細胞においてホメオボックス型転写因子MSX1はコレステロール合成関連遺伝子の発現を制御する, 五藤紀子、藤本勝巳、藤井紗貴子、依田浩子、大島勇人、河本健、能城光秀、宿南知佐、香西克之、加藤幸夫, 第38回日本分子生物学会年会第88回日本生化学会合同大会, 2015年12月02日, 通常, 日本語, 神戸

受賞

  1. 2000年07月, 優秀ポスター賞, 日本骨代謝学会, bHLH 型転写因子 DEC2 のクローニング

外部資金

競争的資金等の採択状況

  1. 科学研究費助成事業(基盤研究(C)), 歯髄で高発現するホメオボックス遺伝子MSXファミリーの機能解析, 2013年, 2015年
  2. 科学研究費助成事業(基盤研究(B)), 転写因子DEC1による血圧制禦機構の解明, 2011年, 2013年
  3. 科学研究費助成事業(基盤研究(C)), 転写因子 Dec2 と Soxファミリー蛋白の相互作用による軟骨分化制御機構, 2010年, 2012年
  4. 科学研究費助成事業(基盤研究(C)), 歯周疾患におけるストレス誘導性タンパク質の関与, 2008年, 2010年
  5. 科学研究費助成事業(基盤研究(A)), 骨・軟骨の分子時計系の解明とその標的遺伝子の同定, 2005年, 2008年
  6. 科学研究費助成事業(基盤研究(B)), 生体時計と骨代謝:新規時計転写因子による骨軟骨形成の促進, 2003年, 2004年
  7. 科学研究費助成事業(基盤研究(C)), bHLH型転写因子DEC2の機能および軟骨分化における役割の解明, 2002年, 2003年
  8. 科学研究費助成事業(奨励研究(A)), 膜結合型トランスフェリン様蛋白質を介する軟骨分化促進機構の解明, 1998年, 1999年

社会活動

委員会等委員歴

  1. 非常勤講師, 2013年04月, 2014年03月, IGL医療専門学校
  2. 非常勤講師, 2014年04月, 2015年03月, IGL医療専門学校
  3. 非常勤講師, 2015年04月, 2016年03月, IGL医療福祉専門学校
  4. 非常勤講師, 2016年04月, 2017年03月, IGL医療福祉専門学校
  5. 非常勤講師, 2017年04月, 2018年03月, IGL医療福祉専門学校
  6. IGL医療福祉専門学校, 2018年04月, 2019年03月, 非常勤講師