野村 渉WATARU NOMURA
Last Updated :2024/11/01
- 所属・職名
- 大学院医系科学研究科(薬) 教授
- メールアドレス
- wnomurahiroshima-u.ac.jp
- 自己紹介
- 研究では主にゲノム編集技術、タンパク質工学、蛍光イメージング技術に興味を持ち、これまでにないような機能性タンパク質の創製やそれらを活用した新しいスクリーニング技術、研究手法の開発、などを目的としています。ゲノム編集技術は様々な生物に利用できる遺伝子書き換える技術で、医療や農畜産業など様々な産業での応用が期待されています。より正確で安全性の高い手法を開発することは応用の幅も拡げることにつながると期待しています。
基本情報
主な職歴
- 2002年04月, 2005年03月, 日本学術振興会, 特別研究員
- 2005年04月, 2007年07月, スクリプス研究所, 博士研究員
- 2007年08月, 2011年12月, 東京医科歯科大学, 助教
- 2012年01月, 2013年03月, 東京医科歯科大学, 講師
- 2013年04月, 2019年03月, 東京医科歯科大学, 准教授
- 2019年04月, 広島大学, 大学院医系科学研究科, 教授
学歴
- 京都大学, 薬学研究科, 生命薬科学専攻博士後期課程, 日本, 2002年04月, 2005年03月
- 京都大学, 薬学研究科, 生命薬科学専攻修士課程, 日本, 2000年04月, 2002年03月
- 京都大学, 薬学部, 薬学科, 日本, 1996年04月, 2000年03月
学位
教育活動
授業担当
- 2024年, 学部専門, 2ターム, 機能形態学
- 2024年, 学部専門, 2ターム, 生化学IV
- 2024年, 学部専門, 3ターム, 薬学概論
- 2024年, 学部専門, セメスター(前期), 薬学研究方法論演習A
- 2024年, 学部専門, セメスター(後期), 薬学研究方法論演習B
- 2024年, 学部専門, セメスター(前期), 細胞薬理学演習
- 2024年, 学部専門, 2ターム, 生理化学
- 2024年, 学部専門, 3ターム, 日本薬局方演習
- 2024年, 学部専門, セメスター(後期), 生物化学実習
- 2024年, 学部専門, 通年, 基礎研究I
- 2024年, 学部専門, 通年, 基礎研究II
- 2024年, 学部専門, セメスター(後期), 基礎研究III
- 2024年, 学部専門, 通年, 臨床研究I
- 2024年, 学部専門, 通年, 臨床研究II
- 2024年, 学部専門, 通年, 臨床研究III
- 2024年, 博士課程・博士課程後期, セメスター(前期), 生理化学特別演習
- 2024年, 博士課程・博士課程後期, セメスター(後期), 生理化学特別演習
- 2024年, 博士課程・博士課程後期, セメスター(前期), 生理化学特別研究
- 2024年, 博士課程・博士課程後期, セメスター(後期), 生理化学特別研究
- 2024年, 修士課程・博士課程前期, セメスター(前期), 薬科学特論
- 2024年, 博士課程・博士課程後期, セメスター(前期), 薬科学特講
- 2024年, 修士課程・博士課程前期, セメスター(前期), 細胞内情報伝達学特論演習
- 2024年, 修士課程・博士課程前期, セメスター(後期), 細胞内情報伝達学特論演習
- 2024年, 修士課程・博士課程前期, セメスター(前期), 細胞内情報伝達学特別研究
- 2024年, 修士課程・博士課程前期, セメスター(後期), 細胞内情報伝達学特別研究
- 2024年, 博士課程・博士課程後期, セメスター(前期), 生理化学特別演習
- 2024年, 博士課程・博士課程後期, セメスター(後期), 生理化学特別演習
- 2024年, 博士課程・博士課程後期, セメスター(前期), 生理化学特別研究
- 2024年, 博士課程・博士課程後期, セメスター(後期), 生理化学特別研究
- 2024年, 博士課程・博士課程後期, 3ターム, ゲノム編集先端研究特論A
研究活動
学術論文(★は代表的な論文)
- Engineering of Zinc Finger Nucleases Through Structural Modeling Improves Genome Editing Efficiency in Cells, ADVANCED SCIENCE, 11巻, 23号, 202406
- CRISPRa Analysis of Phosphoinositide Phosphatases Shows That TMEM55A Is a Positive Regulator of Autophagy, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 47巻, 6号, pp. 1148-1153, 202406
- Discovery of Potent DAG-Lactone Derivatives as HIV Latency Reversing Agents, ACS INFECTIOUS DISEASES, 10巻, 6号, pp. 2250-2261, 20240521
- Effect of Two-Photon Excitation to 8-Azacoumarin Derivatives as Photolabile Protecting Groups, CHEMICAL & PHARMACEUTICAL BULLETIN, 72巻, 3号, pp. 311-312, 202403
- Effects of Polymeric Materials on Activation of THP-1 Cell-derived Macrophages during Differentiation Induced by PMA, ADVANCED BIOMEDICAL ENGINEERING, 13巻, pp. 1-10, 2024
- Evaluation of Proinflammatory Response to Polymeric Materials Using a Macrophage Cell Line Genetically Tagged with a Luminescent Peptide, ADVANCED BIOMEDICAL ENGINEERING, 13巻, pp. 43-51, 2024
- SpCas9-HF1 enhances accuracy of cell cycle-dependent genome editing by increasing HDR efficiency, and by reducing off-target effects and indel rates, MOLECULAR THERAPY NUCLEIC ACIDS, 35巻, 1号, 20240312
- Development of the 8-aza-3-bromo-7-hydroxycoumarin-4-ylmethyl group as a new entry of photolabile protecting groups, Tetrahedron, 70巻, 29号, pp. 4400-4404, 20140722
- Development of a fluoride-responsive amide bond cleavage device that is potentially applicable to a traceable linker, Tetrahedron, 70巻, 34号, pp. 5122-5127, 20140826
- Development of site-specific DNA methylase for epigenetic regulation of gene expression, Seikagaku, 82巻, 5号, pp. 393-397, 20101201
- Significant effect of linker sequence on DNA recognition by multi-zinc finger protein, Biochemical and Biophysical Research Communications, 282巻, 4号, pp. 1001-1007, 20010101
- Multiconnection of identical zinc finger: Implication for DNA binding affinity and unit modulation of the three zinc finger domain, Biochemistry, 40巻, 9号, pp. 2932-2941, 20010306
- The history of genome editing: advances from the interface of chemistry & biology, CHEMICAL COMMUNICATIONS, 59巻, 50号, pp. 7676-7684, 20230620
- The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications, JOURNAL OF BIOLOGICAL INORGANIC CHEMISTRY, 28巻, 3号, pp. 249-261, 202304
- Cas9-Geminin and Cdt1-fused anti-CRISPR protein synergistically increase editing accuracy, FEBS LETTERS, 597巻, 7号, pp. 985-994, 202304
- Interconversion between serine and aspartic acid in the alpha helix of the N-terminal zinc finger of Sp1: implication for general recognition code and for design of novel zinc finger peptide recognizing complementary strand., Biochemistry, 41巻, 28号, 2002
- Influence of TFIIIA-type linker at the N- or C-terminal of nine-zinc finger protein on DNA-binding site., Biochemical and biophysical research communications, 300巻, 1号, 2003
- Effects of length and position of an extended linker on sequence-selective DNA recognition of zinc finger peptides., Biochemistry, 42巻, 50号, 2003
- Effects of linking 15-zinc finger domains on DNA binding specificity and multiple DNA binding modes., Bioorganic & medicinal chemistry letters, 15巻, 9号, 2005
- Design and synthesis of artificial zinc finger proteins., Methods in molecular biology (Clifton, N.J.), 352巻, 2007
- In vivo site-specific DNA methylation with a designed sequence-enabled DNA methylase., Journal of the American Chemical Society, 129巻, 28号, 2007
- Fluorophore labeling enables imaging and evaluation of specific CXCR4-ligand interaction at the cell membrane for fluorescence-based screening., Bioconjugate chemistry, 19巻, 9号, 2008
- Structure-activity relationship study of CXCR4 antagonists bearing the cyclic pentapeptide scaffold: identification of the new pharmacophore., Organic & biomolecular chemistry, 6巻, 23号, 2008
- Synthesis of protein kinase Cdelta C1b domain by native chemical ligation methodology and characterization of its folding and ligand binding., Journal of peptide science : an official publication of the European Peptide Society, 15巻, 10号, 2009
- Structure-activity relationship study on artificial CXCR4 ligands possessing the cyclic pentapeptide scaffold: the exploration of amino acid residues of pentapeptides by substitutions of several aromatic amino acids., Organic & biomolecular chemistry, 7巻, 18号, 2009
- Exploratory studies on development of the chemokine receptor CXCR4 antagonists toward downsizing., Perspectives in medicinal chemistry, 2巻, 2008
- Fluorogenically active leucine zipper peptides as tag-probe pairs for protein imaging in living cells., Angewandte Chemie (International ed. in English), 48巻, 48号, 2009
- CD4 mimics targeting the mechanism of HIV entry., Bioorganic & medicinal chemistry letters, 20巻, 1号, 2010
- Remodeling of dynamic structures of HIV-1 envelope proteins leads to synthetic antigen molecules inducing neutralizing antibodies., Bioconjugate chemistry, 21巻, 4号, 2010
- [Development of site-specific DNA methylase for epigenetic regulation of gene expression]., Seikagaku. The Journal of Japanese Biochemical Society, 82巻, 5号, 2010
- Development of crosslink-type tag-probe pairs for fluorescent imaging of proteins., Biopolymers, 94巻, 6号, 2010
- Peptide HIV-1 integrase inhibitors from HIV-1 gene products., Journal of medicinal chemistry, 53巻, 14号, 2010
- Peptidic HIV integrase inhibitors derived from HIV gene products: structure-activity relationship studies., Bioorganic & medicinal chemistry, 18巻, 18号, 2010
- CD4 mimics targeting the HIV entry mechanism and their hybrid molecules with a CXCR4 antagonist., Bioorganic & medicinal chemistry letters, 20巻, 19号, 2010
- Bivalent ligands of CXCR4 with rigid linkers for elucidation of the dimerization state in cells., Journal of the American Chemical Society, 132巻, 45号, 2010
- Fluorescent-responsive synthetic C1b domains of protein kinase Cδ as reporters of specific high-affinity ligand binding., Bioconjugate chemistry, 22巻, 1号, 2011
- Azamacrocyclic metal complexes as CXCR4 antagonists., ChemMedChem, 6巻, 5号, 2011
- Intense blue fluorescence in a leucine zipper assembly., Chembiochem : a European journal of chemical biology, 12巻, 5号, 2011
- Fluorescence-quenching screening of protein kinase C ligands with an environmentally sensitive fluorophore., Bioconjugate chemistry, 22巻, 5号, 2011
- Small molecular CD4 mimics as HIV entry inhibitors., Bioorganic & medicinal chemistry, 19巻, 22号, 2011
- Synthetic caged DAG-lactones for photochemically controlled activation of protein kinase C., Chembiochem : a European journal of chemical biology, 12巻, 4号, 2011
- A synthetic C34 trimer of HIV-1 gp41 shows significant increase in inhibition potency., ChemMedChem, 7巻, 2号, 2012
- Conjugation of cell-penetrating peptides leads to identification of anti-HIV peptides from matrix proteins., Bioorganic & medicinal chemistry, 20巻, 4号, 2012
- Effects of DNA binding of the zinc finger and linkers for domain fusion on the catalytic activity of sequence-specific chimeric recombinases determined by a facile fluorescent system., Biochemistry, 51巻, 7号, 2012
- Evaluation of a synthetic C34 trimer of HIV-1 gp41 as AIDS vaccines., Bioorganic & medicinal chemistry, 20巻, 10号, 2012
- Pharmacophore-based small molecule CXCR4 ligands., Bioorganic & medicinal chemistry letters, 22巻, 12号, 2012
- The successes and failures of HIV drug discovery., Expert opinion on drug discovery, 6巻, 10号, 2011
- Low-molecular-weight CXCR4 ligands with variable spacers., ChemMedChem, 8巻, 1号, 2013
- A future perspective on the development of chemokine receptor CXCR4 antagonists., Expert opinion on drug discovery, 3巻, 10号, 2008
- CD4 mimics as HIV entry inhibitors: lead optimization studies of the aromatic substituents., Bioorganic & medicinal chemistry, 21巻, 9号, 2013
- Multimerized CHR-derived peptides as HIV-1 fusion inhibitors., Bioorganic & medicinal chemistry, 21巻, 15号, 2013
- Peptide-based ligand screening and functional analysis of protein kinase C., Biopolymers, 100巻, 6号, 2013
- Cell-permeable stapled peptides based on HIV-1 integrase inhibitors derived from HIV-1 gene products., ACS chemical biology, 8巻, 10号, 2013
- Anti-HIV-1 peptide derivatives based on the HIV-1 Co-receptor CXCR4., ChemMedChem, 8巻, 10号, 2013
- CXCR4-derived synthetic peptides inducing anti-HIV-1 antibodies., Bioorganic & medicinal chemistry, 21巻, 22号, 2013
- A CD4 mimic as an HIV entry inhibitor: pharmacokinetics., Bioorganic & medicinal chemistry, 21巻, 24号, 2013
- Development of a traceable linker containing a thiol-responsive amino acid for the enrichment and selective labelling of target proteins., Organic & biomolecular chemistry, 12巻, 23号, 2014
- Screening for protein kinase C ligands using fluorescence resonance energy transfer., Chemical & pharmaceutical bulletin, 62巻, 10号, 2014
- [Chemical biology for pharmaceutical science--integrative approaches for elucidation of biological phenomena]., Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 135巻, 3号, 2015
- [Application and potential of genome engineering by artificial enzymes]., Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan, 135巻, 3号, 2015
- An in-cell fluorogenic Tag-probe system for protein dynamics imaging enabled by cell-penetrating peptides., Bioconjugate chemistry, 26巻, 6号, 2015
- Anti-HIV screening for cell-penetrating peptides using chloroquine and identification of anti-HIV peptides derived from matrix proteins., Bioorganic & medicinal chemistry, 23巻, 15号, 2015
- Trivalent ligands for CXCR4 bearing polyproline linkers show specific recognition for cells with increased CXCR4 expression., Organic & biomolecular chemistry, 13巻, 32号, 2015
- Exploration of labeling by near infrared dyes of the polyproline linker for bivalent-type CXCR4 ligands., Bioorganic & medicinal chemistry, 23巻, 21号, 2015
- Utilization of the Heavy Atom Effect for the Development of a Photosensitive 8-Azacoumarin-Type Photolabile Protecting Group., Organic letters, 17巻, 21号, 2015
- Multimerized HIV-gp41-derived peptides as fusion inhibitors and vaccines., Biopolymers, 106巻, 4号, 2016
- A minimally cytotoxic CD4 mimic as an HIV entry inhibitor., Bioorganic & medicinal chemistry letters, 26巻, 2号, 2016
- Small-Molecule CD4 Mimics Containing Mono-cyclohexyl Moieties as HIV Entry Inhibitors., ChemMedChem, 11巻, 8号, 2016
- Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization., Bioorganic & medicinal chemistry, 24巻, 16号, 2016
- Development of anti-HIV peptides based on a viral capsid protein., Biopolymers, 108巻, 1号, 2017
- Bivalent 14-mer peptide ligands of CXCR4 with polyproline linkers with anti-chemotactic activity against Jurkat cells., Journal of peptide science : an official publication of the European Peptide Society, 23巻, 7-8号, 2017
- Microwave-Assisted Synthesis of Azacoumarin Fluorophores and the Fluorescence Characterization., The Journal of organic chemistry, 82巻, 5号, 2017
- Synthesis and Evaluation of Dimeric Derivatives of Diacylglycerol-Lactones as Protein Kinase C Ligands., Bioconjugate chemistry, 28巻, 8号, 2017
- Synthesis of a Chloroalkene Dipeptide Isostere-Containing Peptidomimetic and Its Biological Application., ACS medicinal chemistry letters, 9巻, 1号, 2018
- [ZIP tag-probe system: A fluorescence imaging tool for intercellular proteins]., Seikagaku. The Journal of Japanese Biochemical Society, 89巻, 1号, 2017
- Delivery of a Proapoptotic Peptide to EGFR-Positive Cancer Cells by a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR., Bioconjugate chemistry, 29巻, 6号, 2018
- Development of Toolboxes for Precision Genome/Epigenome Editing and Imaging of Epigenetics., Chemical record (New York, N.Y.), 18巻, 12号, 2018
- Efficient and Orthogonal Transcription Regulation by Chemically Inducible Artificial Transcription Factors., Biochemistry, 57巻, 45号, 2018
- Inhibition of EGFR Activation by Bivalent Ligands Based on a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR., Chemical & pharmaceutical bulletin, 66巻, 11号, 2018
- Design, synthesis and biological evaluation of low molecular weight CXCR4 ligands., Bioorganic & medicinal chemistry, 27巻, 6号, 2019
- Dimeric C34 Derivatives Linked through Disulfide Bridges as New HIV-1 Fusion Inhibitors., Chembiochem : a European journal of chemical biology, 20巻, 16号, 2019
- Introducing "Future of Biochemistry 2020: The Asia-Pacific Issue", BIOCHEMISTRY, 59巻, 1号, pp. 1-7, 2020
- ★, TALEN-Based Chemically Inducible, Dimerization-Dependent, Sequence-Specific Nucleases, Biochemistry, 59巻, 2号, pp. 197-204, 20200121
- Synthesis of hydrophilic caged DAG-lactones for chemical biology applications, Organic and Biomolecular Chemistry, 18巻, 22号, pp. 4217-4223, 20200614
- Development of a NanoBRET-Based Sensitive Screening Method for CXCR4 Ligands, BIOCONJUGATE CHEMISTRY, 30巻, 5号, pp. 1442-1450, 2019
- A minimally cytotoxic CD4 mimic as an HIV entry inhibitor, Bioorganic and Medicinal Chemistry Letters, 26巻, 2号, pp. 397-400, 20160115
- Small-Molecule CD4 Mimics Containing Mono-cyclohexyl Moieties as HIV Entry Inhibitors, ChemMedChem, 11巻, 8号, pp. 940-946, 20160419
- Multimerized HIV-gp41-derived peptides as fusion inhibitors and vaccines, Biopolymers, pp. 622-628, 20161104
- Development of anti-HIV peptides based on a viral capsid protein, Biopolymers, 108巻, 1号, 20170101
- Microwave-Assisted Synthesis of Azacoumarin Fluorophores and the Fluorescence Characterization, Journal of Organic Chemistry, 82巻, 5号, pp. 2739-2744, 20170303
- Bivalent 14-mer peptide ligands of CXCR4 with polyproline linkers with anti-chemotactic activity against Jurkat cells, Journal of Peptide Science, 23巻, 7-8号, pp. 574-580, 20170701
- Synthesis and Evaluation of Dimeric Derivatives of Diacylglycerol-Lactones as Protein Kinase C Ligands, Bioconjugate Chemistry, 28巻, 8号, pp. 2135-2144, 20170816
- Creation of functional molecules based on biomolecular interactions; development toward chemical biology, Yakugaku Zasshi, 137巻, 10号, pp. 1223-1231, 20170101
- ZIP tag-probe system: A fluorescence imaging tool for intercellular proteins, Seikagaku, 89巻, 1号, pp. 115-120, 20170101
- Synthesis of a Chloroalkene Dipeptide Isostere-Containing Peptidomimetic and Its Biological Application, ACS Medicinal Chemistry Letters, 9巻, 1号, pp. 7-11, 20180111
- Delivery of a Proapoptotic Peptide to EGFR-Positive Cancer Cells by a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR, Bioconjugate Chemistry, 29巻, 6号, pp. 2050-2057, 20180620
- Development of Toolboxes for Precision Genome/Epigenome Editing and Imaging of Epigenetics, Chemical Record, 18巻, 12号, pp. 1717-1726, 20181201
- Inhibition of EGFR activation by bivalent ligands based on a cyclic peptide mimicking the dimerization arm structure of EGFR, Chemical and Pharmaceutical Bulletin, 66巻, 11号, pp. 1083-1089, 20180101
- Efficient and Orthogonal Transcription Regulation by Chemically Inducible Artificial Transcription Factors, Biochemistry, 57巻, 45号, pp. 6452-6459, 20181113
- Benzolactam-related compounds promote apoptosis of HIV-infected human cells via protein kinase C–induced HIV latency reversal, Journal of Biological Chemistry, 294巻, 1号, pp. 116-129, 20190104
- Design, synthesis and biological evaluation of low molecular weight CXCR4 ligands, Bioorganic and Medicinal Chemistry, 27巻, 6号, pp. 1130-1138, 20190315
- Development of a NanoBRET-based sensitive screening method for CXCR4 ligands, Bioconjugate Chemistry, 30巻, 5号, pp. 1442-1450, 20190515
- Dimeric C34 Derivatives Linked through Disulfide Bridges as New HIV-1 Fusion Inhibitors, ChemBioChem, 20巻, 16号, pp. 2101-2108, 20190101
- ★, A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy, Communications Biology, 3巻, 1号, 20201201
- Discovery of a Macropinocytosis-Inducing Peptide Potentiated by Medium-Mediated Intramolecular Disulfide Formation, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 60巻, 21号, pp. 11928-11936, 20210517
- ★, Molecular Switch Engineering for Precise Genome Editing, BIOCONJUGATE CHEMISTRY, 32巻, 4号, pp. 639-648, 20210421
- Fluorescence resonance energy transfer-based screening for protein kinase C ligands using 6-methoxynaphthalene-labeled 1,2-diacylglycerol-lactones, ORGANIC & BIOMOLECULAR CHEMISTRY, 19巻, 38号, pp. 8264-8271, 20211006
著書等出版物
- 2022年05月12日, BIOINDUSTRY【特集】ペプチド医薬の最新動向 ~広い観点からのペプチド創薬科学~, HIV-1阻害剤・腫瘍認識プローブとしてのケモカイン受容体リガンド, CMC出版, 2022年, 05, 単行本(学術書), 共著, 日本語, 野村渉、玉村啓和
受賞
- 2023年01月28日, 第61回日本薬学会・日本薬剤師会・日本病院薬剤師会中国四国支部学術大会 2022年度学生発表奨励賞, 公益社団法人日本薬学会中国四国支部支部長, CRISPRa/iによる Anti-CRISPR/CRISPR Cas9 相互作用解析
- 2016年09月17日, 日本薬学会関東支部奨励賞, 日本薬学会関東支部, 生体分子間相互作用を基盤とする機能分子の創製とケミカルバイオロジーへの展開
- 2013年11月08日, 日本ペプチド学会奨励賞, 日本ペプチド学会, ペプチド性化合物の分子間相互作用を基盤としたプローブおよび阻害剤の創製