Makoto Fujie

Last Updated :2020/09/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Associate Professor
Other Contact Details
TEL : (+81)82-424-7750 FAX : (+81)82-424-7750
plant physiologist

Basic Information

Major Professional Backgrounds

  • 1995/04/01, 2001/03/31, Hiroshima University, Faculty of Engineering, Assistant Professor
  • 2001/04/01, 2009/10/30, Hiroshima University, Graduate School of Advanced Science of Matter, Assistant Professor
  • 2009/11/01, 2019/03/31, Hiroshima University, Graduate School of Advanced Science of Matter, Associate Professor

Educational Backgrounds

  • The University of Tokyo, Graduate School, Division of Science, Japan, 1992/04, 1995/03
  • The University of Tokyo, Faculty of Science, Japan, 1986/04, 1990/03

Academic Degrees

  • Ph. D., The University of Tokyo
  • Master of Science, The University of Tokyo

Research Fields

  • Biology;Basic biology;Morphology / Structure

Research Keywords

  • symbiosis|
  • cell morphology
  • Rhizobium
  • cellfusion
  • mitochondrion
  • cell biology
  • plastid
  • micro algae
  • plant pathogen

Educational Activity

Course in Charge

  1. 2020, Liberal Arts Education Program1, 4Term, Industry and Technology
  2. 2020, Liberal Arts Education Program1, 3Term, Sport fishing and science: fishes-human beings interactions
  3. 2020, Undergraduate Education, Second Semester, Basic Experiments in Chemistry
  4. 2020, Undergraduate Education, Second Semester, Experiments on Biotechnology II
  5. 2020, Undergraduate Education, 2Term, MicrobiologyII
  6. 2020, Graduate Education (Master's Program) , First Semester, Seminar on Molecular Biotechnology
  7. 2020, Graduate Education (Master's Program) , Second Semester, Seminar on Molecular Biotechnology
  8. 2020, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  9. 2020, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  10. 2020, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  11. 2020, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  12. 2020, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  13. 2020, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  14. 2020, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  15. 2020, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  16. 2020, Graduate Education (Master's Program) , 2Term, Basics and Practical Applications of Genome Editing

Research Activities

Academic Papers

  1. Fluorescence microscopic study of the formation of giant mitochondria nuclei in the young ovules of Pelargonium zonale., Protoplasma, 158, 191-194, 19900401
  2. Application of embedding of samples in Technovit 7100 resin to observations of small amounts of DNA in the cellular organelles associated with cytoplasmic in hesitance., Applied Fluorescence Technology, 3, 23-25, 19910401
  3. Studies on the behavior of mitochondrial DNA. Synthesis of mitochondrial DNA occurs actively in a specific region just above the quiescent centre in the root meristem of Pelargonium zonale., Journal of Cell Science, 101, 483-493, 19920401
  4. Preferential mitochondrial and plastids DNA synthesis before multiple cell divisions in Nicotiana tabacum, Journal of Cell Science, 103, 831-837, 19920401
  5. Studies on the behavior of organelles and their nucleoids in the root apical meristem of Arabidopsis thaliana (L. ) col., Planta, 189, 443-452, 19930401
  6. Organelle DNA synthesis in the quiescent centre of Arabidopsis thaliana (col.), Journal of Experimental Botany, 44, 689-693, 19930401
  7. Organelles and their nucleoids in the shoot apical meristem during leaf development in Arabidopsis thaliana L., Planta, 194, 395-405, 19940401
  8. Changes in cytoplasmic rRNA density in the root apical meristem observed by high resolution in situ hybridization, Cytologia, 60, 149-158, 19950401
  9. Zepp, a LINE-like elements at the termini of the Chlorella chromosome I, Nucleic Acids Symp. Ser., 35, 305-306, 19960401
  10. Horizontal transmission of group I introns mediated by viruses, Nucleic Acids Symp. Ser., 35, 197-198, 19960401
  11. Alternative expression of a chitosanase gene produces two different proteins in cells infected with Chlorella virus CVK2., Virology, 230, 361-368, 19970401
  12. Proteolytic processing of Chlorella virus CVK2 capsid proteins., Virology, 227, 252-254, 19970401
  13. Molecular mechanism of the alternative expression of the Chlorella virus CVK2 chitosanase gene., Nucleic Acids Symp. Ser., 37, 141-142, 19970401
  14. Zepp, a LINE-like retrotransposon accumulated in the Chlorella telomeric region., EMBO J., 16(12), 3715-3723, 19970401
  15. Designing of plant artificial chromosomes (PAC) by using the Chlorella smallest chromosome as a model system., Nucleic Acids Symp. Ser., 37, 143-144, 19970401
  16. Phylogeny of bacterial symbionts of the leguminous tree Acasia mangium., J. Ferm. Bioeng., 84(6), 511-518, 19970401
  17. Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a symbiont of Astagalus sinicus cv. Japan, Journal of Fermentation and Bioengineering, 84(1), 511-518, 19970401
  18. Changes in the Extent of the condensation of nuclear chromatin and the localization of RNA during pollen development in Nicotiana tabacum., Cytologia, 62(2), 121-132, 19970401
  19. Group I introns found in Chlorella viruses: Biological implications., Virology, 242, 319-326, 19980401
  20. Chlorella viruses encode their own tRNAs for protein synthesis., Nucleic Acids Symp. Ser., 39, 259-260, 19980401
  21. Molecular anatomy of a small chromosome in the green alga Chlorella vulgaris., Nucleic Acids Research, 17, 3900-3907, 19980401
  22. Structure of the Chlorella Zepp retrotransposon: nested Zepp clusters in the genome., Mol. Gen. Genet., 259, 256-263, 19980401
  23. Location of cDNA clones on the Chlorella chromosome I contig., Nucleic Acids Symp. Ser., 39, 183-184, 19980401
  24. AsNODc22, a novel nodulin gene of Astragalus sinicus, encodes a protein that localizes along the cell wall of bacterial-infected cells in a nodule., Plant Cell Physiol., 39, 846-852, 19980401
  25. Detection and quantification of rRNA by high-resolution in situ hybridizaion in pollen grains., Journal of Plant Research, 111, 45-52, 19980401
  26. A High Density of rRNA in the Generative Cells and Sperm Cells of Pollen Grains of Five Angiosperm Species, Cytologia, 63(3), 293-300, 19980925
  27. Cloning and alaysis of a nodulin gene which encodes protease of Astragalus sinicus (Renge-sou), Plant and cell physiology, 40, s160-s160, 19990301
  28. Aminoacylation of tRNAs encoded by Chlorella virus CVK2., Virology, 263, 220-229, 19990401
  29. Genetic variation of Chlorella viruses: variable regions localized on the CVK2 genomic DNA., Virology, 255, 376-384, 19990401
  30. Phylogenetic position of Mesorhizobium huakuii subsp. rengei, a symbiont of Astragalus sinicus cv. Japan., J. Biosci. Bioeng., 87(1), 49-55, 19990401
  31. Expression of a chitinase gene and lysis of the host cell wall during Chlorella virus CVK2 infection., Virology, 260, 308-315, 19990401
  32. Two catalytic domains of Chlorella virus CVK2 chitinase, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 89(3), 252-257, 20000301
  33. The involvement of a cysteine proteinase in the nodule development in Chinese milk vetch infected with Mes(]E87EE[)hizobium huakii subsp rengei., Plant Physiology, 124, 1087-1095, 20000401
  34. Algal-lytic activities encoded by Chlorella virus CVK2., Virology, 211, 119-126, 20000401
  35. Two catalytic domains of Chlorella virus CVK2 chitinase., J. Biosci. Bioeng., 89(3), 252-257, 20000401
  36. Characterization of immediate early genes expressed in Chlorella virus infection., Nucleic Acids Symp. Ser., 44, 161-162, 20000401
  37. Mapping of cDNA clones on the contig of Chlorella chromosome I., J. Biosci. Bioeng., 90(4), 431-436, 20000401
  38. Analysis of double-strand-break repair by Chlorella retrotransposon Zepp., Nucleic Acids Symp. Ser., 44, 101-102, 20000401
  39. Molecular cloning and characterization of two cDNAs encoding asparagine synthetase from Astragalus sinicus nodules., J. Biosci. Bioeng., 89(6), 559-563, 20000401
  40. Digestion of Chlorella cells by chlorovirus-encoded polysaccharide degrading enzymes., Microbes and Environmants, 16(4), 197-205, 20010401
  41. Chitin synthesis in chlorovirus CVK2-infected Chlorella cells., Virology, 302, 123-131, 20020331
  42. A variable region on the Chlorovirus CVK2 genome contains five copies of the gene for Vp260, a viral-surface glycoprotein., Virology, 295, 289-298, 20020331
  43. Two ftsZ genes of Mesorhizobium huakuii, Biotechnology for Sustainable Utilization of Biological Resources in the Tropics, 15, 242-247, 20020401
  44. Retrotransposon-mediated restoration of Chlorella telomeres: accumulation of Zepp retroransposons at termini of newly formed minichromosomes., Nucleic Acids Res., 31, 4646-4653, 20030401
  45. Minichromosome formation in Chlorella cells irradiated with electron beams, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95(6), 601-607, 20030601
  46. Immediate early genes expressed in chlorovirus infections, VIROLOGY, 318(1), 214-223, 20040105
  47. Vp130, a chloroviral surface protein that interacts with the host chlorella cell wall, Virology, 319, 71-80, 20040201
  48. Isolation of high quality RNA from high-phenolic tissues of eelgrass (Zostera marina L.) by keeping temperature low., Plant Mol Biol Rep, 23(4), 1a-1h, 20051201
  49. Cloning and characterization of a plasma membrane H+-ATPase (zha2) from eelgrass (Zostera marinaL.), Proc. 13th Austr. Plant Greed. Conf., 13, 585-595, 20060501
  50. ZWHA-B1, the gene for subunit B of vacuolar H+-ATPase from the eelgrass Zostera marina L. is able to replace vma2 in a yeast null mutant, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 102(5), 390-395, 20061101
  51. New bacteriophages that infect the phytopathogen Ralstonia solanacearum., Microbiology, 153, 2630-2639, 20070701
  52. Genomic characterization of the filamentous integrative Bacteriophages phi RSSI and phi RSMI, which infect Ralstonia solanacearum, JOURNAL OF BACTERIOLOGY, 189(16), 5792-5802, 20070801
  53. Monitoring of phytopathogenic Ralstonia solanacearum cells using green fluorescent protein-expressing plasmid derived from bacteriophage phi RSS1, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(6), 451-456, 20071201
  54. Genomic characterization of Ralstonia solanacearum phage phi RSA1 and its related prophage (phi RSX) in strain GMI1000, JOURNAL OF BACTERIOLOGY, 190(1), 143-156, 20080101
  55. Genomic Characterization of Ralstonia solanacearum Phage phi RSB1, a T7-Like Wide-Host-Range Phage, JOURNAL OF BACTERIOLOGY, 191(1), 422-427, 20090101
  56. Host recognition and integration of filamentous phage phi RSM in the phytopathogen, Ralstonia solanacearum, VIROLOGY, 384(1), 69-76, 20090205
  57. Molecular Cytological Analysis of Cysteine Proteinases from Nodules of Lotus japonicus, CYTOLOGIA, 74(3), 343-354, 20090901
  58. Monitoring growth and movement of Ralstonia solanacearum cells harboring plasmid pRSS12 derived from bacteriophage phi RSS1, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109(2), 153-158, 20100201
  59. A jumbo phage infecting the phytopathogen Ralstonia solanacearum defines a new lineage of the Myoviridae family, VIROLOGY, 398(1), 135-147, 20100301
  60. Resolvase-like serine recombinase mediates integration/excision in the bacteriophage phi RSM, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 111(2), 109-116, 20110201
  61. Biocontrol of Ralstonia solanacearum by Treatment with Lytic Bacteriophages, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(12), 4155-4162, 20110601
  62. The Filamentous Phage phi RSS1 Enhances Virulence of Phytopathogenic Ralstonia solanacearum on Tomato, PHYTOPATHOLOGY, 102(3), 244-251, 20120301
  63. Loss of Virulence of the Phytopathogen Ralstonia solanacearum Through Infection by phi phi RSM Filamentous Phages, PHYTOPATHOLOGY, 102(5), 469-477, 20120501
  64. Utilization of Filamentous Phage phi RSM3 to Control Bacterial Wilt Caused by Ralstonia solanacearum, PLANT DISEASE, 96(8), 1204-1209, 20120801
  65. Hyaluronan synthesis in cultured tobacco cells (BY-2) expressing a chlorovirus enzyme: Cytological studies, BIOTECHNOLOGY AND BIOENGINEERING, 110(4), 1174-1179, 20130401
  66. Prolonged synthesis of hyaluronan by Chlorella cells infected with chloroviruses, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 115(5), 527-531, 20130501
  67. Characterization of Bacteriophages Cp1 and Cp2, the Strain-Typing Agents for Xanthomonas axonopodis pv. citri, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(1), 77-85, 20140101
  68. The filamentous phage XacF1 causes loss of virulence in Xanthomonas axonopodis pv. citri, the causative agent of citrus canker disease, FRONTIERS IN MICROBIOLOGY, 5(321), 1-11, 20140601
  69. Insights into the diversity of phi RSM phages infecting strains of the phytopathogen Ralstonia solanacearum complex: regulation and evolution, MOLECULAR GENETICS AND GENOMICS, 289(4), 589-598, 20140801
  70. Isolation of Ralstonia solanacearum-infecting bacteriophages from tomato fields in Chiang Mai, Thailand, and their experimental use as biocontrol agents, JOURNAL OF APPLIED MICROBIOLOGY, 118(4), 1023-1033, 20150401
  71. The involvement of the PilQ, secretin of type IV pili in phage infection in Ralstonia solanacearum, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 469(4), 868-872, 20160122
  72. Genomic diversity of large-plaque-forming podoviruses infecting the phytopathogen Ralstonia solanacearum, VIROLOGY, 492, 73-81, 20160501
  73. Two asian jumbo phages, phi RSL2 and phi RSF1, infect Ralstonia solanacearum and show common features of phi KZ-related phages, VIROLOGY, 494, 56-66, 20160601
  74. Replications of Two Closely Related Groups of Jumbo Phages Show Different Level of Dependence on Host-encoded RNA Polymerase, FRONTIERS IN MICROBIOLOGY, 8(1010), 1-11, 20170613
  75. Dynamic integration and excision of filamentous phage XacF1 in Xanthomonas citri pv. citri, the causative agent of citrus canker disease, FEBS OPEN BIO, 7(11), 1715-1721, 20171101
  76. Lysogenic Conversion of the Phytopathogen Ralstonia solanacearum by the P2virus phi RSY1, FRONTIERS IN MICROBIOLOGY, 8(2212), 1-11, 20171114
  77. Improved Sensitivity for High Resolution in Situ Hybridization Using Resin Extraction of Methyl Methacrylate Embedded Material, Biotechnic & Histochemistry, 74(1), 40-48, 19990101
  78. AL-1, a novel polysaccharide lyase encoded by chlorovirus CVK2, FEBS Letters., 559, 51-56, 20040106
  79. Chitin synthesis by &ITChlorella&IT cells infected by chloroviruses: Enhancement by adopting a slow-growing virus and treatment with aphidicolin, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(3), 311-315, 201803

Invited Lecture, Oral Presentation, Poster Presentation

  1. The broad host range of Escherichia phage EcS1 is explained by its multivalent adsorption apparatus, Alaaeldin M. Saad, Takeru Kawasaki, Miyako Nakano, Makoto Fujie and Takashi Yamada, 2019 MOLECULAR GENETICS OF BACTERIA AND PHAGES MEETING, 2019/08/06, Without Invitation, University of Wisconsin-Madison, Madison, Wisconsin, USA, About the function of Novel Jumjbo Phage
  2. Use of bacteriophage for control of bacterial wilt disease caused by Development of real-time monitoring system for Ralstonia solanacearum using plasmid modified from Phage RSS and analysis of bacterial movement in planta Ralstonia solanacearum, Makoto Fujie, Use of bacteriophage for control of bacterial wilt disease caused by Ralstonia solanacearum, 2015/09/11, With Invitation, BIOTEC Thailand, Center for Agricultural biotechnology Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom, Thailand
  3. Importance of PilQ in Ralstonia solanacearum on the infection by several types of bacteriophage, Erlia Narulita, Takeru Kawasaki, Makoto Fujie, Takashi Yamada, 2015/10/27, Without Invitation
  4. The expression and subcellular localization of nodule specific proteases from Lotus japonicus., Makoto Fujie, Plant Cell Signaling 2017, 2017/07/04, With Invitation, International Photosynthesis Industrialization Research Centre, Kitakyushu, Japan, Leguminous plants establish symbioses with soil bacteria to establish symbiotic nitrogen fixation. Many nodule-specific genes known as nodulins have been isolated from various legumes to study their functions in the root nodule developmental process. We isolated more than 100 clones from Chinese milk vetch, Astragalus sinicus (Fujie et al. 1998, Kasai et al. 2000, Naito et al. 2000). and found that AsNodf32 encodes a cysteine protease of the papain super family (Naito et al. 2000). AsNodf32 was mainly expressed in a senescent zone and the expression was also observed transiently in the inter zone before differentiation (Naito et al. 2000). The AsNodf32 is similar to AgNOD-CP1 gene of Alnus glutinosa, including a putative vacuolar-targeting signal, LQDA, and AgNOD-CP1 gene is expressed in the nodule specifically (Goetting-Minesky and Mullin 1994). Other cysteine proteases that are expressed in the nodule are also reported, such as PsCyp1 and Cyp15a (Kardailsky and Brewin 1996). The expression of PsCyp1 and Cyp15a is associated with senescence (Sheokand and Brewin 2003). Recently, Lotus japonicus has been widely used as a model leguminous plant. In this study, we have used L. japonicus to investigate the functions of AsNodf32 using molecular genetic methods. Homologues of AsNodf32, GNf037h07, GNf089d01, GNf032f12 and GNf071h01, were identified in the EST library of the L. japonicus and their expressions in the course of development were investigated by qRT-PCR. The expression patterns of the four genes were different each other. To investigate the functions of the genes during nodule development, precise expression patterns and localization in the nodule were studied by histochemical method using GUS as a reporter. The promoter sequences of the four proteinases were ligated to the GUS genes, respectively and the cassettes were cloned into pUB-GFP plasmid. L. japonicus were transformed with the plasmids by hairy root method using Agrobacterium rhizogenes LBA1334 and transformed roots identified by the GFP fluorescence were inoculated with Mesorhizobium loti MAFF303099 to get transformed nodules. The expression of the four genes were examined by GUS staining. The expression of GNf037h07 was relatively weak and its signal was observed in parenchyma surrounding symbiotic cells. The signals of GNf089d01 was not detected by GUS staining. GNf032f12 was strongly expressed in the infected region. Sometimes the signal of GNf032f12 was observed in vascular bundle at base of nodules. The expression of GNf071h01 was detected in the vascular bundles in the nodules, at the base of nodules and in the root close to the nodules. These expression patterns suggest that the functions of the four proteases were differentiated during evolution. The subcellular localization of the LjCyp2 (protease coded by GNf037h07) was also studied using cultured cells, tobacco BY-2, since BY-2 cells are much suitable for cytological observation than using leguminous plants. In BY-2 cells, the localization of the LjCyp2-GFP-fusion protein changed depending on the cell growth phase. GFP fluorescence was observed in the cytoplasm of rapidly growing cells while the fluorescence was observed solely in the vacuoles of cells at the stationary phase. Cyclical changes of the localization observed in BY-2 cells may reflect control mechanism of LjCyp2 functions in the nodule.

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI, 2015, 2016
  2. KAKENHI, 2012, 2014
  3. KAKENHI, The analysis of movement ability of Ralstonia solanacearum in planta, 2011, 2013
  4. KAKENHI, Characterization of φRSL1-encoded genes controlling the host virulence, 2009, 2011
  5. KAKENHI, 2006, 2007
  6. 2004
  7. KAKENHI, 2003, 2003
  8. KAKENHI, Characterization of novel viral enzymes to be utilized in recycling of biomass, 2001, 2002
  9. KAKENHI, 2000, 2000
  10. KAKENHI, 1999, 2000
  11. KAKENHI, 1997, 1998