TAKESHI IKEDA

Last Updated :2019/02/01

Affiliations, Positions
Graduate School of Advanced Sciences of Matter, ., Assistant Professor
E-mail
ikedatakeshihiroshima-u.ac.jp

Basic Information

Academic Degrees

  • Doctor of Agriculture, The University of Tokyo
  • Master of Agriculture, The University of Tokyo

Research Fields

  • Agricultural sciences;Agricultural chemistry;Applied biochemistry

Research Keywords

  • biosensing
  • fusion tag
  • semiconductor
  • affinity purification
  • silicon-binding (silica-binding) protein
  • biomineralization

Educational Activity

Course in Charge

  1. 2018, Undergraduate Education, Second Semester, Basic Experiments in Chemistry
  2. 2018, Undergraduate Education, First Semester, Training of Biotechnology I
  3. 2018, Graduate Education (Master's Program) , First Semester, Seminar on Molecular Biotechnology
  4. 2018, Graduate Education (Master's Program) , Second Semester, Seminar on Molecular Biotechnology
  5. 2018, Graduate Education (Master's Program) , Year, Advanced Study in Molecular Biotechnology I
  6. 2018, Graduate Education (Master's Program) , Academic Year, Advanced Study in Molecular Biotechnology I

Research Activities

Academic Papers

  1. Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(5), 633-638, 2016
  2. The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat, JOURNAL OF BACTERIOLOGY, 198(2), 276-282, 2016
  3. Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(12), 5677-5684, 2014
  4. Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization, ANALYTICAL METHODS, 6(16), 6232-6235, 2014
  5. Why does the silica-binding protein Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces", COLLOIDS AND SURFACES B-BIOINTERFACES, 86(2), 359-363, 2011
  6. The silica-binding Si-tag functions as an affinity tag even under denaturing conditions, PROTEIN EXPRESSION AND PURIFICATION, 77(2), 173-177, 2011
  7. Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner, PROTEIN EXPRESSION AND PURIFICATION, 71(1), 91-95, 2010
  8. Enzymatic and electron paramagnetic resonance studies of anabolic pyruvate synthesis by pyruvate:ferredoxin oxidoreductase from Hydrogenobacter thermophilus, FEBS Journal, 277(2), 501-510, 2010
  9. Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A, ANALYTICAL BIOCHEMISTRY, 385(1), 132-137, FEB 1 2009

Patented

  1. Patent, US:8283443, 2012/10/09, Method for purifying protein