YOSHIAKI NAKAMURA

Last Updated :2019/03/01

Affiliations, Positions
Graduate School of Biosphere Science, ., Assistant Professor
E-mail
ynsuhiroshima-u.ac.jp
Other Contact Details
1-4-4 Kagamiyama, Higashi-Hiroshima City Hiroshima, Japan 739-8528, Japan
TEL : (+81)82-424-7943 FAX : (+81)82-424-7943
Self-introduction
The purpose of my research is to establish technologies for developmental engineering that are robustly available across the animal species, and to apply for the genetic resource preservation at a cellular level as well as for the human infertility clinic. At present, I am tackling the preservation of fertility as well as efficient production of sperm and eggs from surrogate parents through the development and improvement of techniques for the manipulation of germline stem cells including culture, cryopreservation and transplantation in birds and mammals.

Basic Information

Major Professional Backgrounds

  • 2009/04/01, 2011/03/31, Shinshu University, Interdisciplinary Graduate School of Science and Technology, JSPS Research Fellow (DC2)
  • 2011/04/01, 2014/03/31, National Institute for Basic Biology, Division of Germ Cell Biology, NIBB Research Fellow
  • 2014/04/01, 2015/03/31, National Institute for Basic Biology, Division of germ Cell Biology, Research Fellow
  • 2015/04/01, 2017/09/30, National Institute for Basic Biology, Division of Germ Cell Biology, JSPS Research Fellow (PD)

Educational Backgrounds

  • Shinshu University, Faculty of Agriculture, Department of Food Production Science, Japan, 2002/04/01, 2006/03/31
  • Shinshu University, Graduate School of Agriculture, Department of Food Production Science (Master's Program), Japan, 2006/04/01, 2008/03/31
  • Shinshu University, Interdisciplinary Graduate School of Science and Technology, Department of Bioscience and Food Production Science (Doctoral Program), Japan, 2008/04/01, 2011/03/31

Academic Degrees

  • Shinshu University
  • Shinshu University

Research Fields

  • Agricultural sciences;Animal life science;Integrative animal science

Research Keywords

  • Primordial Germ Cell
  • Spermatogenic Stem Cell
  • Transplantation
  • Chimera
  • Cryopreservation
  • Animal Genetic Resources
  • Human Infertility Clinic
  • Chicken
  • Mouse

Affiliated Academic Societies

  • Japanese Society of Animal Science, 2009/06
  • The Society for Reproduction and Development, 2009/05
  • Japan Poultry Science Association, 2006/07
  • Japanese Society of Developmental Biologists, 2006/01

Educational Activity

Course in Charge

  1. 2018, Undergraduate Education, 2Term, Genetics
  2. 2018, Undergraduate Education, Intensive, Laboratory Work in General Biology I
  3. 2018, Undergraduate Education, Intensive, Laboratory Work in General Biology II
  4. 2018, Undergraduate Education, Academic Year, Graduation Thesis

Research Activities

Academic Papers

  1. Cryopreservation of primordial germ cells (PGCs) from White Leghorn embryos using commercial cryoprotectants., Journal of Poultry Science, 44(1), 73-77, 2007
  2. A novel method to isolate primordial germ cells and its use for the generation of germline chimeras in chicken., Biology of Reproduction, 77(1), 115-119, 2007
  3. Migration and proliferation of primordial germ cells in the early chicken embryo., Poultry Science, 86(10), 2182-2193, 2007
  4. Increased proportion of donor primordial germ cells in chimeric gonads by sterilisation of recipient embryos using busulfan sustained-release emulsion in chickens., Reproduction, Fertility and Development, 20(8), 900-907, 2008
  5. Novel system for degeneration of blood vessels by UV irradiation and subsequent regeneration using chick bone marrow cells., Cells Tissues Organs, 189(5), 348-355, 2008
  6. Effects of busulfan sustained-release emulsion on depletion and repopulation of primordial germ cells in early chicken embryos., Journal of Poultry Science, 46(2), 127-135, 2009
  7. Depletion of primordial germ cells (PGCs) by X-irradiation to extraembryonic region of chicken embryos and expression of xenotransplanted quail PGCs., Journal of Poultry Science, 46(2), 136-143, 2009
  8. Analysis of developmental changes in avian DNA methylation using a novel method for quantifying genome-wide DNA methylation., Journal of Poultry Science, 46(4), 286-290, 2009
  9. Current states for regeneration of individuals via germline chimeras by transferring germ cells in animals., Journal of Animal Genetics, 37(1), 41-58, 2009
  10. A novel concentrating system of chicken stem cells by bone marrow side population cells., Journal of Poultry Science, 47(1), 53-56, 2010
  11. Germline replacement by transfer of primordial germ cells into partially sterilized embryos in the chicken., Biology of Reproduction, 83(1), 53-56, 2010
  12. Efficient system for preservation and regeneration of genetic resources in chicken: concurrent storage of primordial germ cells and live animals from early embryos of a rare indigenous fowl (Gifujidori)., Reproduction, Fertility and Development, 22(8), 1237-1246, 2010
  13. Viability and functionality of primordial germ cells after freeze-thaw in chickens., Journal of Poultry Science, 48(1), 57-63, 2011
  14. Comparison of liver mitochondrial proteins derived from newborn cloned calves and from cloned adult cattle by two-dimensional differential gel electrophoresis., Molecular Reproduction & Development, 78(4), 263-273, 2011
  15. Improving rates of germline transmission in chimeric chickens using busulfan solubilised sustained-release emulsion., Avian Biology Research, 4(2), 74-77, 2011
  16. Identification of germline chimeric chickens produced by transfer of primordial germ cells using a Hinai-dori-specific microsatellite marker., Journal of Poultry Science, 48(4), 281-291, 2011
  17. Comparison of liver mitochondrial proteins derived from newborn cloned calves and from cloned adult cattle by two-dimensional differential gel electrophoresis., Journal of Reproduction and Development, 58(2), 248-253, 2012
  18. X-irradiation removes endogenous primordial germ cells (PGCs) and increases germline transmission of donor PGCs in chimeric chickens., Journal of Reproduction and Development, 58(4), 432-437, 2012
  19. Development, differentiation and manipulation of chicken germ cells., Development, Growth & Differentiation, 55(1), 20-40, 2013
  20. Culture conditions for maintain propagation, long-term survival and germline transmission of chicken primordial germ cell-like cells., Journal of Poultry Science, 51(1), 87-95, 2014
  21. Production of pure Hinai-dori with normal reproductive capability from transferred primordial germ cells., Journal of Poultry Science, 51(3), 297-306, 2014
  22. Production of functional gametes from cryopreserved primordial germ cells of the Japanese quail., Journal of Reproduction and Development, 59(6), 580-587, 2013
  23. Production of functional gametes following transfer of frozen-thawed primordial germ cells of Hinai-dori fowl after long distance transportation: For diversification of the risk to outbreaks of highly pathogenic avian influenza., Japanese Journal of Poultry Science, 53(J1), J7-J14, 2016
  24. Poultry genetic resource conservation using primordial germ cells., Journal of Reproduction and Development, 62(5), 431-437, 2016
  25. The extended brown plumage color mutant of blue-breasted quail (Coturnix chinensis) is associated with the mutation in the melanocortin 1-receptor gene (MC1R), Journal of Poultry Science, 55(4), 233-238, 20181025
  26. Developmental stages of the blue-breasted quail (Coturnix chinensis), Animal Science Journal, 90(1), 35-48, 20190104
  27. Skeletal development in blue-breasted quail embryos, Animal Science Journal, 2018

Publications such as books

  1. 2017, Avian Primordial Germ Cells. In: Avian Reproduction: From Behavior to Molecules (Sasanami T eds.), This book provides everything from basic knowledge to the recent understandings of avian reproductive physiology, covering many unique aspects. It will inspire avian biologists as well as researchers in varied fields and will offer important steps towards better fertilization success in birds.In spite of the recent remarkable developments in modern technology, a comprehensive understanding of the reproductive mechanisms is still far in the future due to the diverse reproductive tactics in vertebrates. Birds have highly refined reproductive strategies and some of those strategies are unique to birds. However, together with ongoing progress of the genome analysis of birds and the crying need for further increase in meat and egg production, research on avian reproduction is now accelerating and becoming more important.With contributions by leading scientists, the book explains avian primordial germ cells; the sex-determining mechanism; reproductive endocrinology and immunology; sperm, egg, and egg coat; sperm–egg interaction; polyspermic fertilization; seasonal reproduction; social triggers; hormonal and behavioral changes; broodiness; oviductal sperm storage; and biotechnology. This book is recommended for all researchers and students who are interested in birds or reproduction., Avian Primordial Germ Cells, Springer Singapore, 2017, E, Tagami T, Miyahara D, Nakamura Y, 978-981-10-3974-4, pp.1-18
  2. 2017, Avian Biotechnology. In: Avian Reproduction: From Behavior to Molecules (Sasanami T eds.), This book provides everything from basic knowledge to the recent understandings of avian reproductive physiology, covering many unique aspects. It will inspire avian biologists as well as researchers in varied fields and will offer important steps towards better fertilization success in birds.In spite of the recent remarkable developments in modern technology, a comprehensive understanding of the reproductive mechanisms is still far in the future due to the diverse reproductive tactics in vertebrates. Birds have highly refined reproductive strategies and some of those strategies are unique to birds. However, together with ongoing progress of the genome analysis of birds and the crying need for further increase in meat and egg production, research on avian reproduction is now accelerating and becoming more important.With contributions by leading scientists, the book explains avian primordial germ cells; the sex-determining mechanism; reproductive endocrinology and immunology; sperm, egg, and egg coat; sperm–egg interaction; polyspermic fertilization; seasonal reproduction; social triggers; hormonal and behavioral changes; broodiness; oviductal sperm storage; and biotechnology. This book is recommended for all researchers and students who are interested in birds or reproduction., Springer Singapore, 2017, E, Nakamura Y, 978-981-10-3974-4, pp.187-214

Invited Lecture, Oral Presentation, Poster Presentation

  1. Primordial germ cell-mediated genetic resource preservation system in chicken., Nakamura Y, Kagami H, Tagami T, Korea-Japan Joint Symposium on Avian Biology 2011, 2011, With Invitation, Seoul national University, Seoul, Republic of Korea
  2. Genetic conservation at the cellular level in chicken., Nakamura Y, 2012 International Symposium on Animal Biotechnology, 2012, With Invitation, Faculty of Agriculture, Shinshu University, Minamiminowa, Nagano, Japan
  3. Post-transplantation dynamics of mouse spermatogenic stem cells: For improvement of its transplantation efficiency., Nakamura Y, Yoshida S, 12th Annual Conference of the Asian Reproductive Biotechnology Society, 2015, With Invitation, Asian Reproductive Biotechnology Society, Hanoi, Vietnam
  4. The distribution pattern of primordial germ cells in early chick embryos., Nakamura Y, Yamamoto Y, Usui F, Ono T, Takeda K, Nirasawa K, Kagami H, Tagami T, Annual Conference of the International Embryo Transfer Society, 2007, Without Invitation, International Embryo Transfer Society, Kyoto, Japan
  5. A novel strategy for preservation of genetic resources in birds., Nakamura Y, Usui F, Yamamoto Y, Atsumi Y, Ono T, Takeda K, Nirasawa K, Kagami H, Tagami T, Society for the Study of Reproduction 41st Annual Meeting, 2008, Without Invitation, Society for the Study of Reproduction, Kailua-Kona, HI, USA
  6. Establishment of a novel strategy for efficient removal of primordial germ cells in early chicken embryos., Nakamura Y, Yamamoto Y, Usui F, Atsumi Y, Ono T, Takeda K, Nirasawa K, Kagami H, Tagami T, XXIII World’s Poultry Congress 2008, 2008, Without Invitation, World Poultry Science Association, Brisbane, Australia
  7. Germline replacement by transferring primordial germ cells into sterilized embryos in the chicken., Nakamura Y, Usui F, Takeda K, Ono T, Nirasawa K, Kagami H, Tagami T., Poultry Science Association 98th Annual Meeting, 2009, Without Invitation, Pourtly Science Association, Raleigh, NC, USA
  8. Gametogenesis following transplantation of primordial germ cells in domestic fowls., NakamuraY, Czech-Japan Joint Symposium for Animal Reproduction 'From Gametes to Stem Cells', 2010, Without Invitation, Society for Reproduction and Development, Liblice, Czech
  9. Effects of X-ray irradiation on primordial germ cell-depletion in chicken embryos., Nakamura Y, Usui F, Miyahara D, Mori T, Watanabe H, Ono T, Takeda K, Nirasawa K, Kagami H, Tagami T, 9th Asia Pacific Poultry Conference, 2011, Without Invitation, Asia Poultry Science Association, Taipei, Taiwan
  10. X ray-irradiation removes endogenous primordial germ cells (PGCs) and increases germline transmission of exogenous PGCs in chicken., Nakamura Y, Usui F, Miyahara D, Mori T, Ono T, Kagami H, Tagami T, World Congress on Reproductive Biology 2011, 2011, Without Invitation, World Congress on Reproductive Biology, Cairns, Australia
  11. Germ-cell transplantation in chicken and its application to gene banking., Nakamura Y, The 58th/60th NIBB Conference Germline -Specification, Sex, and Stem Cells-, 2012, Without Invitation, National Institute for Basic Biology, Okazaki, Japan
  12. Continuous observation of donor germ cells after spermatogonial transplantation in mice., Nakamura Y, Yoshida S, Cold Spring Harbor Laboratory 2012 Meeting on Germ Cells, 2012, Without Invitation, Cold Spring Harbor Laboratory, Laurel Hollow, NY, USA
  13. Stochastic behaviors of mouse spermatogenic stem cells after transplantation., Nakamura Y, Hara K, Simons BD, Yoshida S, Gordon Research Conference 2014 Mammalian Reproduction, 2014, With Invitation, Gordon Research Conference, New London, NH, USA
  14. Single-cell level dynamics of mouse spermatogenic stem cells after transplantation., Nakamura Y, Yoshida S, World Congress on Reproductive Biology 2014, 2014, Without Invitation, World Congress on Reproductive Biology, Edinburgh, Scotland.
  15. Post-transplantation fates of individual mouse spermatogenic stem cells., Nakamura Y, Yoshida S, Cold Spring Harbor Laboratory 2016 Meeting on Germ Cells, 2016, Without Invitation, Cold Spring Harbor Laboratory, Laurel Hollow, NY, USA
  16. Understanding the post-transplantation behavior of mouse spermatogenic stem cells., Nakamura Y, Yoshida S, World Congress on Reproductive Biology 2017, 2017, Without Invitation, World Congress on Reproductive Biology, Okinawa, Japan
  17. Augment of transplantation by modulating behavior of spermatogenic stem cells., Nakamura Y, RCAS/JAB International symposium on Animal Biotechnology: from Genes to Cells, 2018/02/21, With Invitation, School of Applied Biological Science, Hiroshima University
  18. Preservation of poultry genetic resources through freezing and tansplantation of germ cells., Yoshiaki Nakamura, The 2018 Annual meeting of The Japan Society for Bioscience, Biotechnology and Agrochemistry, 2018/03/18, With Invitation, Japan Society for Bioscience, Biotechnology and Agrochemistry, Nagoya
  19. Germline Chimera production by transfer of germ cells in poultry and rodents., Yoshiaki Nakamura, 111 th Annual meeting of the Society for the Reproduction and Development, 2018/09/12, With Invitation, the Society for the Reproduction and Development, Ueda, Nagano

Awards

  1. 2007年, JPSA Best Presentation Award (2007), Japan Poultry Science Association
  2. 2008年, Faculty of Agriculture, Shinshu University's for Distiguished Service Award (2008), Faculty of Agriculture, Shinshu University
  3. 2009年, SRD Best Presentation Award (2009), Society for Reproduction and Development
  4. 2009年, JSAS Best Presentation Award (2009), Japanese Society of Animal Science
  5. 2010年, The Journal of Poultry Science Outstanding Paper Award (2010), Japan Poultry Science Association
  6. 2010年, Best Poster Award in the Czech-Japan Joint Symposium for Animal Reproduction "From Gametes to Stem Cells" (2010), Society for Reproduction and Development
  7. 2011年, Shinshu University's Distiguished Service Award (2011), Shinshu University
  8. 2012年, JPSA Young Investigator Award (2012), Japan Poultry Science Association
  9. 2015年, SRD Young Investigator Award (2015), Society for Reproduction and Development
  10. 2016年, SRD Best Presentation Award (2016), Society for Reproduction and Development
  11. 2017年, Best Poster Award in the International Research Symposium on Regulation of Germ Cell Development in vivo and in vitro (2017), Scientific Research on Innovative Areas "Mechanisms regulating gamete formation in animals"
  12. 2018年11月30日, Japan Prize in Agricultural Science, Achievement Award for Young Scientists, The foundation of Agricultural Science of Japan, Establishment of a system for cryopreservation of poultry genetic resources using primordial germ cells.

External Funds

Acceptance Results of Competitive Funds

  1. Grant-in-Aid for JSPS Research Fellow, Regeneration of rare avian species via germline chimeras., 2009/04, 2011/03
  2. 24th Kato Memorial Grant for International Excahange, Continuous observation of donor germ cells after spermatogonial transplantation in mice., 2012/10
  3. Grant-in-Aid for Young Scientists (B), Homing mechanisms of mouse spermatogenic stem cells following transplantation., 2013/04, 2015/03
  4. Grant-in-Aid for JSPS Research Fellow, 2015/04, 2018/03
  5. Grant-in-Aid for Challenging Research, 2015/04, 2017/03
  6. 2016/04, 2017/03
  7. 28th Kato Memorial Grant for International Excahange, Post-transplantation fates of individual mouse spermatogenic stem cells., 2016/09
  8. Grant-in-Aid for Scientific Research on Innovative Areas, 2016/04, 2018/03
  9. 2017/04, 2018/03
  10. 2017 Kieikai Research Foundation, 2017/04
  11. Grants-in-Aid for Scientific Research for Early-Career Scientists, 2018/04/01, 2021/03/31
  12. AMED National Bioresource Project: Fundamental technology upgrading program, Sophistication of the cryopreservation of avian germ cells., 2018/07/01, 2020/03/31
  13. Grant-in-Aid for Scientific Research on Innovative Areas, 2018/06/29, 2023/03/31
  14. Grant-in-Aid for Scientific Research on Innovative Areas, Ensuring integrity in gametogenesis, 2018/07/01, 2023/03/31

Social Activities

History as Committee Members

  1. JSAS Young Scientist Committee, 2012/06, 2017/03, Japan Society of Animal Science
  2. JSAS Young Scientist Committee (Chair), 2017/04, Japan Society of Animal Reproduction
  3. JPSA Young Scientist Committee, 2012/07, Japan Poultry Science Association
  4. councilor, 2018/04, Japan Poultry Science Association

Organizing Academic Conferences, etc.

  1. 111 th Annual Meeting of the Society for the Reproduction and Development, 2017/09, 2018/09
  2. Japan Society of Animal Sciece 3rd Young Researcher's Science Camp, 2018/08

Other Social Contributions

  1. 2010 JICA Group Training Course (Poultry Production, Management and Disease Prevention Technology), Poultry New Technology, JICA, 2010/12, Nishigo, Fukushima
  2. 2011 JICA Group Training Course (Poultry Production, Management and Disease Prevention Technology), Poultry New Technology, JICA, 2011/12, Nishigo, Fukushima
  3. 2012 JICA Group Training Course (Poultry Production, Management and Disease Prevention Technology), Poultry New Technology, JICA, 2012/12, Nishigo, Fukushima
  4. 2013 JICA Group Training Course, Poultry New Technology, JICA, 2013/11/11, 2013/11/12, Nishigo, Fukushima
  5. Invited seminar in Zhejiang Academy of Agricultural Science, Dynamics of spermatogenic stem cells after transplantation in mice., Zhejiang Academy of Agricultural Science, 2012/09, Hangzhou, China
  6. Invited seminar in The Roslin Institute, Germ cell transplantation in chicken and mouse, The Roslin Institute, 2015/10, Edinburgh, UK
  7. Satellite Workshop, Live Hands-on Primordial Germ Cells isolation, vitrification and transplantation, 12th Annual International Conference of the Asian Reproductive Biotechnology Society, 2015/11, Hanoi, Vietnam
  8. 38th annual seminar of Grant-in-Aid for Scientific Research on Innovative Areas "Mechanisms regulating gamate formation in animals", Germ cell transplantation in avian and mamals for cryopreservation of animal genetic resources., Grant-in-Aid for Scientific Research on Innovative Areas "Mechanisms regulating gamate formation in animals", 2017/04, Yokohama, Kanagawa
  9. Invited seminar in Seoul National University, Behavior of mouse spermatogenic stem cells after transplantation, Department of Agricultural Biotechnology, Seoul National University, 2018/05/16, Pyeongchang, Republic of Korea
  10. 9th seminar for consotium researchers, My experiences of winning Kaken-hi, HIRAKU consotium, 2018/06/25, Higashi-Hiroshima, Hiroshima