Keisuke Nakajima

Last Updated :2019/04/09

Affiliations, Positions
Institute for Amphibian Biology, Institute for Amphibian Biology, Assistant Professor

Basic Information

Major Professional Backgrounds

  • 1993/04/01, 2001/08/31, Tokyo Metropolitan Institute for Neruoscience, Department of Molecular Neurobiology
  • 2001/09/01, 2007/03/31, Hiroshima University, Insitute for Amphibian Biology, Graduate School of Science, Research Associate

Educational Backgrounds

  • Waseda University, Graduate School, Division of Natural Science, Japan, 1991/04, 1993/03
  • Waseda University, Faculty of Education, Japan, 1987/04, 1991/03

Academic Degrees

  • Ph.D., Waseda University
  • Master of Science, Waseda University

Research Fields

  • Biological Sciences;Laboratory animal science;Laboratory animal science

Research Keywords

  • muscle
  • Xenopus
  • apoptosis

Educational Activity

Course in Charge

  1. 2019, Liberal Arts Education Program1, 1Term, Exercise in Information Literacy
  2. 2019, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
  3. 2019, Undergraduate Education, Second Semester, Seminar for Amphibian Biology
  4. 2019, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  5. 2019, Undergraduate Education, First Semester, Practice for Fundamental Biology III
  6. 2019, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  7. 2019, Graduate Education (Doctoral Program) , 2Term, Seminar for Advanced Research in Basic Biology E
  8. 2019, Graduate Education (Doctoral Program) , 3Term, Seminar for Advanced Research in Basic Biology F

Research Activities

Academic Papers

  1. Thyroid Hormone Receptor alpha- and beta-Knockout Xenopus tropicalis Tadpoles Reveal Subtype-Specific Roles During Development, ENDOCRINOLOGY, 159(2), 733-743, 201802
  2. An Inhibitor of Thyroid Hormone Synthesis Protects Tail Skin Grafts Transplanted to Syngenic Adult Frogs, ZOOLOGICAL SCIENCE, 34(5), 414-418, 201710
  3. no privacy, a Xenopus tropicalis mutant, is a model of human Hermansky-Pudlak Syndrome and allows visualization of internal organogenesis during tadpole development, DEVELOPMENTAL BIOLOGY, 426(2), 472-486, 20170615
  4. Mechanisms of tail resorption during anuran metamorphosis, Biomolecular Concepts, 8(3-4), 179-183, 2017
  5. Ouro proteins are not essential to tail regression during Xenopus tropicalis metamorphosis, GENES TO CELLS, 21(3), 275-286, 201603
  6. Generation of Albino Cynops pyrrhogaster by Genomic Editingof the tyrosinase Gene, ZOOLOGICAL SCIENCE, 33(3), 290-294, 201606
  7. Development of a new approach for targeted gene editing in primordial germ cells using TALENs in Xenopus, BIOLOGY OPEN, 4(3), 259-266, 20150315
  8. Highly efficient gene knockout by injection of TALEN mRNAs into oocytes and host transfer in Xenopus laevis, BIOLOGY OPEN, 4(2), 180-185, 20150215
  9. Xenopus pax6 mutants affect eye development and other organ systems, and have phenotypic similarities to human aniridia patients, Developmental Biology, 408(2), 328-344, 2015
  10. Expression of the amelogenin Gene in the Skin of Xenopus tropicalis, ZOOLOGICAL SCIENCE, 30(3), 154-159, 201303
  11. Targeted Gene Disruption in the Xenopus tropicalis Genome Using Designed TALE Nucleases, ZOOLOGICAL SCIENCE, 30(6), 455-460, 201306
  12. Comparison of TALEN scaffolds in Xenopus tropicalis, BIOLOGY OPEN, 2(12), 1364-1370, 20131215
  13. Generation of albino Xenopus tropicalis using zinc-finger nucleases, DEVELOPMENT GROWTH & DIFFERENTIATION, 54(9), 777-784, 201212
  14. Regulation of thyroid hormone sensitivity by differential expression of the thyroid hormone receptor during Xenopus metamorphosis, GENES TO CELLS, 17(8), 645-659, 201208
  15. Translational regulation by the 5 '-UTR of thyroid hormone receptor alpha mRNA, JOURNAL OF BIOCHEMISTRY, 151(5), 519-531, 2012
  16. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, EXPERIMENTAL ANIMALS, 59(4), 395-405, 201007
  17. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 49(2), 131-143, 2007
  18. One of the duplicated matrix metalloproteinase-9 genes is expressed in regressing tail during anuran metamorphosis, DEVELOPMENT GROWTH & DIFFERENTIATION, 48(4), 223-241, 200605
  19. Programmed cell death during amphibian metamorphosis, SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY, 16(2), 271-280, 200504
  20. Involvement of caspase-9 in execution of the maternal program of apoptosis in Xenopus late blastulae overexpressed with S-ademosylmethionine decarbosylase, Biochemical and Biophysical Research Communications, 325(4), 1367-1375, 20041201
  21. Dual mechanisms governing muscle cell death in tadpole tail during amphibian metamorphosis, DEVELOPMENTAL DYNAMICS, 227(2), 246-255, 200306
  22. Structure, Expression and Function of the Xenopus laevis Caspase Family, Journal of Biological Chemistry, 275(14), 10484-10491, 20000401
  23. Construction of multiple-epitope tag sequence by PCR for sensitive Western blot analysis, Nucleic Acids Research, 25(11), 2231-2232, 19970601
  24. Induction of apoptosis and CPP32 expression by thyroid hormone in a myoblastic cell line derived from tadpole tail, Journal of Biological Chemistry, 272(8), 5122-5127, 19970201
  25. Absence of Methylated Thyrotropin-Releasing Hormone in the Bullfrog (Rana catesbeiana) Brain, Zoological Science, 10, 711-714, 19931001
  26. Thyrotropin-releasing hormone (TRH) is the major prolactin-releasing factor in the bullfrog hypothalamus, General and Comparative Endocrinology, 89(1), 11-16, 19930101
  27. Xenopus tropicalis: An Ideal Experimental Animal in Amphibia, Proceedings of The Japanese Society of Animal Models for Human Diseases, 59(4), 395-405, 2010

Publications such as books

  1. 1998/10, 両生類の変態とアポトーシス , 医歯薬出版株式会社, 1998, 10, 6
  2. 1996/11, 変態とアポトーシス , 羊土社, 1996, 11, 2

Invited Lecture, Oral Presentation, Poster Presentation

  1. Two unique TALEN methods for the highly efficient mutagenesis and genomic editing preferentially in germ cell using Xenopus, K. Nakajima and Y. Yaoita, K. Nakajima and Y. Yaoita, World Congress of Molecular & Cell Biology-2016, 2016/04, With Invitation, Dalian, China
  2. The role of the thyroid hormone receptor during Xenopus metamorphosis, Keisuke Nakajima, Keisuke Nakajima, Kenta Fujimoto and Yoshio Yaoita, 8th International Symposium on Amphibian and Reptilian Endocrinology and Neurobiology, 2014/11, With Invitation
  3. Xenopus tropicalis, a model organism for the new genetics era: from forward to reverse genetics and now to gene targeting, Takuya Nakayama, Takuya Nakayama, Margaret B. Fish, Marilyn Fisher, Keisuke Nakajima, Yoshio Yaoita and Robert M. Grainger, International Symposium Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, 2014/03, With Invitation
  4. ネッタイツメガエルにおけるノックアウト法の活用, Keisuke Nakajima, Keisuke Nakajima, Yuya Nakai, Morihiro Okada, Minoru Takase, Yoshio Yaoita, 第83回日本動物学会, 2012, With Invitation
  5. Low levels of Thyroid Hormone can Induce the Death of Thyroid Hormone Receptor- Overexpressing Muscle Cells in Xenopus Tadpole Tail, Keisuke Nakajima, Nakajima, K., Fujimoto, K., and Yaoita, Y, 2009/12, With Invitation
  6. Expression of matrix metalloproteinase genes in regressing or remodeling organs during amphibian metamorphosis, Yoshio Yaoita, Kenta Fujimoto, Keisuke Nakajima and Yoshio Yaoita, 2007/03, With Invitation
  7. The regulation of thyroid hormone sensitivity of the Xenopus tadpole tail, Kenta Fujimoto, Kenta Fujimoto, Keisuke Nakajima, Yoshio Yaoita, 2006/05, With Invitation
  8. Molecular mechanism of tail muscle cell death in regressing tail during amphibian metamorphosis, Yoshio Yaoita, Yoshio Yaoita, Kenta Fujimoto and Keisuke Nakajima, NIH seminar, 2005/09, With Invitation
  9. The analysis of a myoblastic cell line derived from the amphibian tadpole tail., Yoshio Yaoita, Yoshio Yaoita, Keisuke Nakajima, 1999/07, With Invitation


  1. 1999年03月, Young scientist award, The Frontiers of the Biology of Amphibia, GENE CLONING AND CHARACTERIZATION OF THE XENOPUS LAEVIS CASPASE FAMILY