HIROYUKI KAMIYA

Last Updated :2024/05/17

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Professor
E-mail
hirokamhiroshima-u.ac.jp
Self-introduction
I am interested in nucleic acids including DNA, the blueprint of life, and study "molecular mechanisms of mutagenesis and carcinogenesis by DNA damage" and "design of DNAs for genome editing" as the main projects.

Basic Information

Academic Degrees

  • Doctor of Philosophy in Pharmaceutical Science, HOKKAIDO UNIVERSITY

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Medicinal Sciences : Medicinal Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Pharmaceutical Sciences

In Charge of Primary Major Programs

  • Pharmaceutical Sciences
  • Medicinal Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Biological pharmacy
  • Environmental science;Environmental analyses and evaluation;Risk sciences of radiation and chemicals
  • Medicine,dentistry, and pharmacy;Pharmacy;Medical pharmacy

Research Keywords

  • DNA damage, DNA repair, genome editing, gene correction

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, 4Term, Pharmaceutical Analysis
  2. 2024, Undergraduate Education, Intensive, Pharmaceutical Analysis
  3. 2024, Undergraduate Education, 2Term, Bio-Analytical Science
  4. 2024, Undergraduate Education, First Semester, Research Practice A
  5. 2024, Undergraduate Education, Second Semester, Research Practice B
  6. 2024, Undergraduate Education, First Semester, Practice of Analytical Drug Discovery and Evaluation
  7. 2024, Undergraduate Education, 3Term, Japanese Pharmacopoeia
  8. 2024, Undergraduate Education, Second Semester, Experiments in Analytical Chemistry
  9. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences I
  10. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences II
  11. 2024, Undergraduate Education, Second Semester, Special laboratory Works in Pharmaceutical Sciences III
  12. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy I
  13. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy II
  14. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy III
  15. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Nucleic Acids Biochemistry
  16. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Nucleic Acids Biochemistry
  17. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Nucleic Acids Biochemistry
  18. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Nucleic Acids Biochemistry
  19. 2024, Graduate Education (Master's Program) , First Semester, Lecture on Medicinal Science
  20. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Medicinal Science
  21. 2024, Graduate Education (Master's Program) , First Semester, Lecture on Nucleic Acids Biochemistry
  22. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Nucleic Acids Biochemistry
  23. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Nucleic Acids Biochemistry
  24. 2024, Graduate Education (Master's Program) , First Semester, Research on Nucleic Acids Biochemistry
  25. 2024, Graduate Education (Master's Program) , Second Semester, Research on Nucleic Acids Biochemistry
  26. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Nucleic Acids Biochemistry for Medicinal Sciences
  27. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Nucleic Acids Biochemistry for Medicinal Sciences
  28. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Nucleic Acids Biochemistry for Medicinal Sciences
  29. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Nucleic Acids Biochemistry for Medicinal Sciences

Research Activities

Academic Papers

  1. Easily-controllable, helper phage-free single-stranded phagemid production system, GENES AND ENVIRONMENT, 44(1), 20221116
    Link to Paper Search Results by DOI
  2. Correction of monomeric enhanced green fluorescent protein (mEGFP) gene by short 5'-tailed duplexes, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 134(3), 175-181, 202209
    Link to Paper Search Results by DOI
  3. Development of a versatile high-throughput mutagenesis assay with multiplexed short-read NGS using DNA-barcoded supF shuttle vector library amplified in E. coli, ELIFE, 11, 20221010
    Link to Paper Search Results by DOI
  4. Biased distribution of action-at-a-distance mutations by 8-oxo-7,8-dihydroguanine, MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 825, 2022
    Link to Paper Search Results by DOI
  5. ★, Paradoxical role of the major DNA repair protein, OGG1, in action-at-a-distance mutation induction by 8-oxo-7,8-dihydroguanine, DNA REPAIR, 111, 202203
    Link to Paper Search Results by DOI
  6. Similar frequency and signature of untargeted substitutions induced by abasic site analog under reduced human APE1 conditions, JOURNAL OF TOXICOLOGICAL SCIENCES, 46(6), 283-288, 202106
    Link to Paper Search Results by DOI
  7. Action-at-a-distance mutations at 5 '-GpA-3 ' sites induced by oxidised guanine in WRN-knockdown cells, MUTAGENESIS, 36(5), 349-357, 202109
    Link to Paper Search Results by DOI
  8. Gene correction by 5 '-tailed duplexes with short editor oligodeoxyribonucleotides, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 132(6), 552-559, 202112
    Link to Paper Search Results by DOI
  9. Conventional plasmid DNAs with a CpG-containing backbone achieve durable transgene expression in mouse liver, JOURNAL OF GENE MEDICINE, 22(1), e3138, 202001
    Link to Paper Search Results by DOI
  10. Single-stranded DNA versus tailed duplex in sequence conversion of lacZalpha DNA, Nucleosides Nucleotides Nucleic Acids, 39(9), 1245-1250, 202009
  11. New indicator Escherichia coli strain for rapid and accurate detection of supF mutations, GENES AND ENVIRONMENT, 42(1), 28, 20200922
    Link to Paper Search Results by DOI
  12. Tyrosyl-DNA phosphodiesterases are involved in mutagenic events at a ribonucleotide embedded into DNA in human cells, PLOS ONE, 15(12), e0244790, 20201231
    Link to Paper Search Results by DOI
  13. High-throughput analysis of DNA repair in microplates towards identification of inhibitors, GENES AND ENVIRONMENT, 42(1), 11, 20200309
    Link to Paper Search Results by DOI
  14. No enhancing effects of plasmid-specific histone acetyltransferase recruitment system on transgene expression in vivo, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 38(12), 942-949, 20191202
    Link to Paper Search Results by DOI
  15. Large deletions and untargeted substitutions induced by abasic site analog on leading versus lagging strand templates in human cells, MUTAGENESIS, 34(5-6), 421-429, 2019
    Link to Paper Search Results by DOI
  16. Reduction of Werner Syndrome Protein Enhances G:C -> A:T Transition byO(6)-Methylguanine in Human Cells, CHEMICAL RESEARCH IN TOXICOLOGY, 31(5), 319-324, 201805
    Link to Paper Search Results by DOI
  17. Effects of mismatches distant from the target position on gene correction with a 5 '-tailed duplex, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(5), 619-623, 201805
    Link to Paper Search Results by DOI
  18. Durable Transgene Expression Driven by CpG-Free and -Containing Promoters in Plasmid DNA with CpG-Free Backbone, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 41(9), 1489-1493, 201809
    Link to Paper Search Results by DOI
  19. Mutations induced by 8-oxo-7,8-dihydroguanine in WRN- and DNA polymerase lambda-double knockdown cells, MUTAGENESIS, 33(4), 301-310, 201807
    Link to Paper Search Results by DOI
  20. Analysis of large deletion mutations induced by abasic site analog in human cells, GENES AND ENVIRONMENT, 40, 20181024
    Link to Paper Search Results by DOI
  21. Enhanced transgene expression by plasmid-specific recruitment of histone acetyltransferase, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 123(3), 277-280, 201703
    Link to Paper Search Results by DOI
  22. Comparison of DNA fragments as donor DNAs upon sequence conversion of cleaved target DNA, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 36(6), 428-434, 2017
    Link to Paper Search Results by DOI
  23. Mutations induced by 8-hydroxyguanine (8-oxo-7,8-dihydroguanine), a representative oxidized base, in mammalian cells., Genes Environment, 39, 2 (6 pages), 201701
  24. In vivo selection of active deoxyribonucleoside kinase by a mutagenic nucleoside analog, JOURNAL OF BIOTECHNOLOGY, 228, 52-57, 20160620
    Link to Paper Search Results by DOI
  25. Insertion and Deletion Mismatches Distant from the Target Position Improve Gene Correction with a Tailed Duplex, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 35(7), 379-388, 2016
    Link to Paper Search Results by DOI
  26. Cleavage of Target DNA Promotes Sequence Conversion with a Tailed Duplex, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 39(8), 1392-1395, 201608
  27. Life Style and Cancer - The Open Symposium of the Japanese Environmental Mutagen Society (JEMS) in 2015, GENES AND ENVIRONMENT, 38, 2016
    Link to Paper Search Results by DOI
  28. Action-at-a-Distance Mutagenesis Induced by Oxidized Guanine in Werner Syndrome Protein-Reduced Human Cells, CHEMICAL RESEARCH IN TOXICOLOGY, 28(4), 621-628, 201504
    Link to Paper Search Results by DOI
  29. Transcription of 4 '-thioDNA templates to natural RNA in vitro and in mammalian cells, CHEMICAL COMMUNICATIONS, 51(37), 7887-7890, 2015
  30. Induction of action-at-a-distance mutagenesis by 8-oxo-7,8-dihydroguanine in DNA pol lambda-knockdown cells, GENES AND ENVIRONMENT, 37, 2015
    Link to Paper Search Results by DOI
  31. Anatomy of plasmid DNAs with anti-silencing elements, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 464(1-2), 27-33, 2014
  32. Suppression of short tract gene conversion in episomal DNA by p53 reduction, Genes Environment, 36(2), 65-68, 2014
  33. In vitro Replication Study of Modified bases in ras Sequences., Pharmaceutical Bulletin, 40(10), 2792-2795, 1992
  34. Mutagenicities of 8-Hydroxyguanine and 2-Hydroxyadenine Produced by Reactive Oxygen Species (Biological and Pharmaceutical Aspects of Nucleic Acids Chemistry), Biological & pharmaceutical bulletin, 27(4), 475-479, 20040401
  35. Pharmacokinetic Modeling of Species-dependent Enhanced Bioavailability of Trifluorothymidine by Thymidine Phosphorylase Inhibitor, Drug Metabolism and Pharmacokinetics, 19(3), 206-215, 2004
  36. Calpain 10 as a Predictive Gene for Type 2 Diabetes : Evidence from a Novel Screening System Using White Blood Cells of Otsuka Long-Evans Tokushima Fatty (OLETF) Rats (Biopharmacy), Biological & pharmaceutical bulletin, 26(12), 1765-1768, 20031201
  37. Mutagenicity of 5-Formylcytosine, an Oxidation Product of 5-Methylcytosine, in DNA in Mammalian Cells, Journal of Biochemistry, 132(4), 551-555, 20021001
  38. 2-Hydroxyadenine in DNA is a Very Poor Substrate of the Escherichia coli MutY Protein, Journal of radiation research, 41(4), 349-354, 200012
  39. Comparison of Oxidation Products from DNA Components by gamma-Irradiation and Fenton-Type Reactions, Journal of radiation research, 38(2), 121-131, 199706
  40. Comparison of Incorporation and Extension of Nucleotides in vitro opposite 8-Hydroxyguanine (7,8-Dihydro-8-oxoguanine) in Hot Spots of the c-Ha-ras Gene, Jpn. J. Cancer Res., 86(3), 270-276, 19950331
  41. Inhibition of c-Ha-ras Gene Expression by Hammerhead Ribozymes Containing a Stable C(UUCG)G Hairpin Loop, Biological & pharmaceutical bulletin, 16(9), 879-883, 19930915
  42. Overproduction of cellular and activated Ha-ras proteins by mutating a synthetic gene., Pharmaceutical Bulletin, 35(12), 4878-4882, 1987
  43. Reduced Plasma Glucose by Asparagine Synthetase Knockdown in the Mouse Liver, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 36(12), 2009-2011, 2013
  44. Correlation between transgen expression and plasmid DNA loss in mouse liver, JOURNAL OF GENE MEDICINE, 15(6-7), 242-248, 2013
  45. Enhanced transgene expression from chromatinized plasmid DNA in mouse liver, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 441(1-2), 146-150, 2013
  46. Effects of Endogenous Proteins and microRNA Target Sequence in a Positive Feedback System, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 35(9), 1534-1538, 2012
  47. Mutagenic Bypass of 8-Oxo-7,8-dihydroguanine (8-Hydroxyguanine) by DNA Polymerase kappa in Human Cells, CHEMICAL RESEARCH IN TOXICOLOGY, 25(8), 1771-1776, 2012
  48. Insights into substrate recognition by the Escherichia coli Orf135 protein through its solution structure, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 420(2), 263-268, 2012
  49. H-1, C-13 and N-15 NMR assignments of the Escherichia coli Orf135 protein, BIOMOLECULAR NMR ASSIGNMENTS, 6(1), 1-4, 2012
  50. CREB-binding protein transcription activation domain for enhanced transgene expression by a positive feedback system, JOURNAL OF BIOTECHNOLOGY, 157(1), 7-11, 2012
  51. Diverse substrate recognition and hydrolysis mechanisms of human NUDT5, NUCLEIC ACIDS RESEARCH, 39(20), 8972-8983, 2011
  52. Effects of insulator cHS4 on transgene expression from plasmid DNA in a positive feedback system, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 112(5), 432-434, 2011
  53. Correction of Frameshift Mutations with Tailed Duplex DNAs, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 34(9), 1465-1468, 2011
  54. Mutagenicity of secondary oxidation products of 8-oxo-7,8-dihydro-2 '-deoxyguanosine 5 '-triphosphate (8-hydroxy-2 '- deoxyguanosine 5 '-triphosphate), MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 714(1-2), 11-16, 2011
  55. Mutagenicity of oxidized DNA precursors in living cells: Roles of nucleotide pool sanitization and DNA repair enzymes, and translesion synthesis DNA polymerases, MUTATION RESEARCH-GENETIC TOXICOLOGY AND ENVIRONMENTAL MUTAGENESIS, 703(1), 32-36, 2010
  56. ★, Positive Feedback System Provides Efficient and Persistent Transgene Expression, MOLECULAR PHARMACEUTICS, 7(4), 1125-1132, 2010
  57. Cytotoxic Effect of Drosophila Deoxynucleoside Kinase Gene on Replicating Plasmid in HeLa Cells, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(7), 1223-1227, 2010
  58. Transgene expression efficiency from plasmid DNA delivered as a complex with histone H3, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 392(1-2), 249-253, 2010
  59. DNA Microarray Analysis of Whole Blood Cells and Insulin-Sensitive Tissues Reveals the Usefulness of Blood RNA Profiling as a Source of Markers for Predicting Type 2 Diabetes, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(6), 1033-1042, 2010
  60. ★, Effects of base excision repair proteins on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with cytosine and adenine, DNA REPAIR, 9(5), 542-550, 2010
  61. Suppression of mutagenesis by 8-hydroxy-2 '-deoxyguanosine 5 '-triphosphate (7,8-dihydro-8-oxo-2 '-deoxyguanosine 5 '-triphosphate) by human MTH1, MTH2, and NUDT5, FREE RADICAL BIOLOGY AND MEDICINE, 48(9), 1197-1201, 2010
  62. Roles of specialized DNA polymerases in mutagenesis by 8-hydroxyguanine in human cells, MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS, 686(1-2), 90-95, 2010
  63. ★, Targeted sequence alteration of a chromosomal locus in mouse liver, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 387(1-2), 180-183, 2010
  64. NUDT5 hydrolyzes oxidized deoxyribonucleoside diphosphates with broad substrate specificity, DNA REPAIR, 8(10), 1250-1254, 2009
  65. PK-PD modeling of 1-(3-C-ethynyl-beta-D-ribo-pentofuranosyl)cytosine and the enhanced antitumor effect of its phospholipid derivatives in long-circulating liposomes, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 377(1-2), 52-59, 2009
  66. Effects of carriers on transgene expression from plasmids containing a DNA sequence with high histone affinity, INTERNATIONAL JOURNAL OF PHARMACEUTICS, 376(1-2), 99-103, 2009
  67. Effects of non-B DNA sequences on transgene expression, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 108(1), 18-21, 2009
  68. Incorporation of 8-hydroxyguanosine (8-oxo-7,8-dihydroguanosine) 5'-triphosphate by bacterial and human RNA polymerases, FREE RADICAL BIOLOGY AND MEDICINE, 46(12), 1703-1707, 2009
  69. Involvement of specialized DNA polymerases in mutagenesis by 8-hydroxy-dGTP in human cells, DNA REPAIR, 8(5), 637-642, 2009
  70. Effects of target sequence and sense versus anti-sense strands on gene correction with single-stranded DNA fragments, JOURNAL OF BIOCHEMISTRY, 144(4), 431-436, 2008
  71. ★, Improved gene correction efficiency with a tailed duplex DNA fragment, BIOCHEMISTRY, 47(33), 8754-8759, 2008
  72. Specificity of mutations induced by incorporation of oxidized dNTPs into DNA by human DNA polymerase eta, DNA REPAIR, 7(3), 497-506, 2008
  73. MITO-Porter: A liposome-based carrier system for delivery of macromolecules into mitochondria via membrane fusion, BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES, 1778(2), 423-432, 2008
  74. Base excision repair enzyme endonuclease III suppresses mutagenesis caused by 8-hydroxy-dGTP, DNA REPAIR, 7(1), 88-94, 2008
  75. UvrA and UvrB enhance mutations induced by oxidized deoxyribonucleotides, DNA REPAIR, 6(12), 1786-1793, 2007
  76. Effects of 8-hydroxy-GTP and 2-hydroxy-ATP on in vitro transcription, FREE RADICAL BIOLOGY AND MEDICINE, 43(5), 837-843, 2007
  77. 2-hydroxy-2 '-deoxyadenosine 5 '-triphosphate enhances A center dot T -> C center dot G mutations caused by 8-hydroxy-2 '-deoxyguanosine 5 '-triphosphate by suppressing its degradation upon replication in a HeLa extract, BIOCHEMISTRY, 46(22), 6639-6646, 2007
  78. ★, Mutagenic effects of 8-hydroxy-dGTP in live mammalian cells, FREE RADICAL BIOLOGY AND MEDICINE, 42(10), 1552-1560, 2007
  79. Efficient and erroneous incorporation of oxidized DNA precursors by human DNA polymerase eta, BIOCHEMISTRY, 46(18), 5515-5522, 2007
  80. The location of the left-handedly curved DNA sequence affects exogenous DNA expression in vivo, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 461(1), 7-12, 2007
  81. DNA microarray analysis of type 2 diabetes-related genes co-regulated between white blood cells and livers of diabetic Otsuka long-evans Tokushima fatty (OLETF) rats, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 30(4), 763-771, 2007
  82. Induction of various mutations during PCRs with manganese and 8-hydroxy-dGTP, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 30(4), 842-844, 2007
  83. Cell cycle dependent transcription, a determinant factor of heterogeneity in cationic tipid-mediated transgene expression, JOURNAL OF GENE MEDICINE, 9(3), 197-207, 2007
  84. Mitochondrial drug delivery and mitochondrial disease therapy - An approach to liposome-based delivery targeted to mitochondria, MITOCHONDRION, 7(1-2), 63-71, 2007
  85. Development of multifunctional envelope type artificial viral like gene delivery system, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 127(10), 1655-1672, 2007
  86. Recognition of nucleotide analogs containing the 7,8-dihydro-8-oxo structure by the human MTH1 protein, JOURNAL OF BIOCHEMISTRY, 140(6), 843-849, 2006
  87. Crystallization and preliminary X-ray analysis of human MTH1 complexed with two oxidized nucleotides, 8-oxo-dGMP and 2-oxo-dATP, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS, 62, 1283-1285, 2006
  88. Silencing of exogenous DNA in cultured cells, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 29(6), 1294-1296, 2006
  89. Effects of overexpression and antisense RNA expression of Orf17, a MutT-type enzyme, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 29(6), 1087-1091, 2006
  90. DNA polymerase λ promotes mutagenesis induced by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine) paired with adenine, Genes Environment, 35(4), 105-109, 2013
  91. A designed curved DNA sequence remarkably enhances transgene expression from plasmid DNA in mouse liver, Gene Ther., 19(8), 828-835, 2012
  92. Human MTH3 (NUDT18) with a distinct enzyme activity cleaving oxidized forms of dGDP and GDP: A comparison with MTH1 and MTH2, J. Biol. Chem., 287(25), 21541-21549, 2012
  93. Unexpectedly weak impacts of decreased p53 and retinoblastoma protein levels on mutagenesis by 8-oxo-7,8-dihydroguanine (8-hydroxyguanine), Genes Environment, 33(3), 103-108, 2011
  94. ★, Transient activation of transgene expression by hydrodynamics-based injection may cause rapid decrease in plasmid DNA expression, Gene Therapy, 14(15), 1152-1159, 2007
  95. ★, Increased SFHR gene correction efficiency with sense single-stranded DNA, J. Gene Med., 7(4), 486-493, 2005
  96. ★, Probing the substrate recognition mechanism of the human MTH1 protein by nucleotide analogs, J. Mol. Biol., 336(4), 843-850, 2004
  97. ★, Mutagenic potentials of damaged nucleic acids produced by reactive oxygen/nitrogen species: Approaches using synthetic oligonucleotides and nucleotides, Nucleic Acids Res., 31(2), 517-531, 2003
  98. Methylglyoxal, an endogenous aldehyde, crosslinks DNA polymerase and the substrate DNA, Nucleic Acids Res., 29(16), 3433-3438, 2001
  99. ★, The oxidized forms of dATP are substrates for the human MutT homologue, the hMTH1 protein, J. Biol. Chem., 274(26), 18201-18205, 1999
  100. ★, Induction of chromosomal gene mutations in Escherichia coli by direct incorporation of oxidatively damaged nucleotides, J. Biol. Chem., 273(18), 11069-11074, 1998
  101. ★, Mutations induced by 2-hydroxyadenine on a shuttle vector during leading and lagging strand syntheses in mammalian cells, Biochemistry, 36(37), 11125-11130, 1997
  102. ★, Formation of 2-hydroxydeoxyadenosine triphosphate, an oxidatively damaged nucleotide, and its incorporation by DNA polymerases, J. Biol. Chem., 270(33), 19446-19450, 1995
  103. 8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene. Effects of sequence contexts on mutation spectra, Carcinogenesis, 16(4), 883-889, 1995
  104. ★, c-Ha-ras containing 8-hydroxyguanine at codon 12 induces point mutations at the modified and adjacent positions, Cancer Res., 52(12), 3483-3485, 1992
  105. Cyclobutane thymine-photodimers in a ras protooncogene hot spot activate the gene by point mutation, Nucleic Acids Res., 21(10), 2355-2361, 1993
  106. Mutations induced by oxidized DNA precursors and their prevention by nucleotide pool sanitization enzymes, Genes Environment, 29(4), 133-140, 200711
  107. Correlation between the phosphohydrolase activity of the Escherichia coli Orf135 (NudG) protein and mutation suppression, Genes Environment, 29(2), 63-66, 200705
  108. Increased A:T→C:G Mutations in the mutT Strain upon 8-Hydroxy-dGTP Treatment : Direct Evidence for MutT Involvement in the Prevention of Mutations by Oxidized dGTP, Journal of Biochemistry, 136(3), 359-362, 20040901
  109. Induction of Transition and Transversion Mutations during Random Mutagenesis PCR by the Addition of 2-Hydroxy-dATP (Biochemistry/Molecular Biology), Biological & pharmaceutical bulletin, 27(5), 621-623, 20040501
  110. In Vivo Mutagenicities of Damaged Nucleotides Produced by Nitric Oxide and Ionizing Radiation(Biochemistry/Molecular Biology), Biological & pharmaceutical bulletin, 28(3), 520-522, 20050301
  111. Effect of Methylated Adenine in Plasmid DNA on Transgene Expression in Mice(Biopharmacy), Biological & pharmaceutical bulletin, 28(10), 2019-2022, 20051001
  112. Induction of Substitution and Deletion Mutations by 2-Hydroxyadenine during Replication in a HeLa Extract, Genes and environment : the official journal of the Japanese Environmental Mutagen Society, 28(3), 92-96, 20060831
  113. Silencing of Exogenous DNA in Cultured Cells(Biopharmacy), Biological & pharmaceutical bulletin, 29(6), 1294-1296, 20060601
  114. New NTP analogs: the synthesis of 4'-thioUTP and 4'-thioCTP and their utility for SELEX, Nucleic Acids Research, 33(9), 2942-2951, 200505
  115. RNA interference induced by siRNAs modified with 4'-thioribonucleosides in cultured mammalian cells, FEBS Letters, 579(14), 3115-3118, 20050606

Publications such as books

  1. 2005/08, Modern biopharmaceuticals, Programmed packaging: A new drug delivery system and its application to gene therapy, Wiley-VCH, 2005, 08, Scholarly Book, Joint work, English, K. Kogure, H. Akita, H. Kamiya, and H. Harashima
  2. 2005, Non-viral Gene Therapy: Gene Design and Delivery, Newly designed DNA fragments for gene correction, Springer-Verlag Tokyo, 2005, Scholarly Book, Joint work, English, H. Kamiya, H. Tsuchiya, and H. Harashima

Invited Lecture, Oral Presentation, Poster Presentation

  1. Abasic site analog in lagging strand template induces mutations more frequently than that in leading strand template, T. Suzuki, Y. Katayama, H. Kamiya, 6th Asian Congress on Environmental Mutagens, 2019/11/18, Without Invitation, English, Asian Association of Environmental Mutagen Societies, Tokyo
  2. OGG1-knockdown increases large deletions but decreases untargeted substitutions induced by 8-oxo-7,8-dihydroguanine, Y. Zaima, T. Suzuki, H. Kamiya, 6th Asian Congress on Environmental Mutagens, 2019/11/18, Without Invitation, English, Asian Association of Environmental Mutagen Societies, Tokyo
  3. The role of P53 oscillations via MDM2-MDMX in DNA damage-induced cell fate decisions, H. Kawai, H. Kamiya, 6th Asian Congress on Environmental Mutagens, 2019/11/18, Without Invitation, English, Asian Association of Environmental Mutagen Societies, Tokyo
  4. Large deletion mutations induced by an abasic site analogue in human cells, H. Kamiya, Y. Katayama, T. Suzuki, and Y. Komatsu, 12th International Conference and 5th Asian Congress on Environmental Mutagens, 2017/11/13, Without Invitation, English, Asian Association of Environmental Mutagen Societies, Incheon, Korea
  5. Cleavage of target DNA promotes sequence conversion with a tailed duplex., H. Kamiya, T. Suzuki, T. Imada, N. Nishigaki, M. Kobayashi, I. Matsuoka, The 43rd International Symposium on Nucleic Acids Chemistry, 2016/09/28, Without Invitation, English, Kumamoto

Patented

  1. Patent, CA 2551696, 2014/05/27, Immunopotentiator and method of enhancing immunological activity using the same
  2. Patent, EP 1702620, 2014/01/08, Immunopotentiator and method of enhancing immunological activity with the same
  3. Patent, CA 2555489, 2012/05/08, Method of converting base in DNA sequence

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (B)), 2019, 2021
  2. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2017, 2019
  3. KAKENHI, 2016, 2018

Social Activities

History as Committee Members

  1. Editorial Board, 2014/01, 2023/12
  2. Editorial Board, 2012/01, 2023/12