Akio Kuroda

Last Updated :2017/11/01

Affiliations, Positions
Graduate School of Advanced Sciences of Matter, ., Professor
Web Site
E-mail
akurodahiroshima-u.ac.jp
Other Contact Details
1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan
TEL : (+81)82-424-7758 FAX : (+81)82-424-7047

Basic Information

Major Professional Backgrounds

  • 2005/04/01, Hiroshima University, Graduate school of advanced sciences of Matter, Professor
  • 1998/01/01, 2005/03/31, Hiroshima University, Graduate school of advanced sciences of Matter, Associated professor
  • 1997/04/01, 1998/01/01, Hiroshima University, Faculty of engineering, Associated professor
  • 1994/04/01, 1997/03/31, Hiroshima University, Faculty of engineering, Assistant professor
  • 1988/04/01, 1994/03/31, Shinshu University, Faculty of textile science and technology, Assistant professor

Educational Backgrounds

  • Osaka University, Graduate School, Division of Engineering, Fermentation technology, Japan, 1986/04, 1988/03
  • Osaka University, Faculty of Engineering, Fermentation technology, Japan, 1982/04, 1986/03

Academic Degrees

  • Doctor of Engineering, Osaka University
  • Master of Engineering, Osaka University

Research Fields

  • Engineering;Process / Chemical engineering;Biofunction / Bioprocess
  • Environmental science;Environmental conservation;Environmental conscious materials and recycle
  • Environmental science;Environmental conservation;Environmental risk control and evaluation
  • Agricultural sciences;Agricultural chemistry;Applied microbiology

Research Keywords

  • protein engineering
  • silicon
  • asbestos
  • polyphosphate
  • phosphite
  • Luciferase
  • biosensing

Affiliated Academic Societies

  • The society for biotechnology, Japan
  • Phosphorus recycling promotion council
  • American society for microbiology

Educational Activity

Course in Charge

  1. 2017, Undergraduate Education, Second Semester, Biotechnology
  2. 2017, Undergraduate Education, Second Semester, Group Discussion of Current Biotechnology Topics
  3. 2017, Graduate Education (Master's Program) , Second Semester, Seminar on Molecular Biotechnology
  4. 2017, Graduate Education (Doctoral Program) , Academic Year, Advanced Study in Molecular BiotechnologyII
  5. 2017, Graduate Education (Master's Program) , First Semester, Special Lecture on Advanced Sciences of Matter
  6. 2017, Graduate Education (Master's Program) , First Semester, Seminar on Molecular Biotechnology
  7. 2017, Graduate Education (Master's Program) , First Semester, Frontier Biological Science
  8. 2017, Graduate Education (Master's Program) , Academic Year, Advanced Study in Molecular Biotechnology I
  9. 2017, Graduate Education (Master's Program) , Year, Advanced Study in Molecular Biotechnology I
  10. 2017, Graduate Education (Master's Program) , Academic Year, Advanced Study in Molecular Biotechnology I
  11. 2017, Graduate Education (Master's Program) , Academic Year, Advanced Study in Molecular Biotechnology I
  12. 2017, Graduate Education (Doctoral Program) , Academic Year, Advanced Study in Molecular BiotechnologyII
  13. 2017, Graduate Education (Doctoral Program) , Academic Year, Advanced Study in Molecular BiotechnologyII
  14. 2017, Graduate Education (Doctoral Program) , Year, Advanced Study in Molecular BiotechnologyII
  15. 2017, Graduate Education (Doctoral Program) , Academic Year, Advanced Study in Molecular BiotechnologyII
  16. 2017, Graduate Education (Doctoral Program) , Academic Year, Advanced Study in Molecular BiotechnologyII

Research Activities

Academic Papers

  1. Detection of chrysotile asbestos by using a chrysotile-binding protein, BIOTECHNOLOGY AND BIOENGINEERING, 99(2), 285-289, FEB 1 2008
  2. Selective Detection of Airborne Asbestos Fibers Using Protein-Based Fluorescent Probes, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 44(2), 755-759, JAN 15 2010
  3. The Si-tag for immobilizing proteins on a silica surface, Biotechnology and Bioengineering, 96, 1023-1029, 20070415
  4. The Si-tag for Immobilizing Proteins on a Silicon Device, Technical Proceedings of the 2007 Nanotechnology Conference and Trade Show, 4, 571-574, 20070301
  5. Fabrication of Si Nanowire Field-Effect Transistor for Highly Sensitive, Label-Free Biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 48(6), JUN 2009
  6. Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner, PROTEIN EXPRESSION AND PURIFICATION, 71(1), 91-95, MAY 2010
  7. Role of inorganic polyphosphate in promoting ribosomal, protein degradation by the ion protease in E-coli, SCIENCE, 293(5530), 705-708, JUL 27 2001
  8. A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling, Biotechnol. Bioeng., 78, 333-338, 20010401
  9. A New Subfamily of Polyphosphate Kinase 2 (Class III PPK2) Catalyzes both Nucleoside Monophosphate Phosphorylation and Nucleoside Diphosphate Phosphorylation, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(8), 2602-2608, APR 2014
  10. Use of an Escherichia coli recombinant producing thermostable polyphoaphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate, Applied and Environmental Microbiology, 73(17), 5676-5678, 20070901
  11. ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell, Biosci. Biotech. Biochem., 68, 1216-1220, 20040401
  12. Increase in bioluminescence intensity of firefly luciferase using genetic modification, Analytical Biochemistry, 366, 131-136, 20070701
  13. Endotoxin assay by bioluminescence using mutant firefly luciferase, ANALYTICAL BIOCHEMISTRY, 397(2), 152-155, FEB 15 2010
  14. Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp strain 4506, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(4), 445-450, APR 2012
  15. Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A, ANALYTICAL BIOCHEMISTRY, 385(1), 132-137, FEB 1 2009
  16. Inhibition of quorum sensing in Pseudomonas aeruginosa by N-acyl cyclopentylamides, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(10), 3183-3188, MAY 2007
  17. Reciprocating-flow ATP amplification system for increasing the number of amplification cycles, ANALYTICAL BIOCHEMISTRY, 395(2), 161-165, DEC 15 2009
  18. Continuous-Flow ATP amplification system on a chip, SENSORS AND ACTUATORS B-CHEMICAL, 142(1), 118-122, OCT 12 2009
  19. Selective Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators, JAPANESE JOURNAL OF APPLIED PHYSICS, 49(4), 2010
  20. Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a phoU Mutant of Escherichia coli, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(4), 865-868, APR 2010
  21. Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109(5), 423-432, MAY 2010
  22. Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes, JOURNAL OF BIOTECHNOLOGY, 148(4), 204-207, AUG 2 2010
  23. The silica-binding Si-tag functions as an affinity tag even under denaturing conditions, PROTEIN EXPRESSION AND PURIFICATION, 77(2), 173-177, JUN 2011
  24. Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators Functionalized with an Immobilized Antibody-Binding Protein, JAPANESE JOURNAL OF APPLIED PHYSICS, 50(4), APR 2011
  25. Sensitivity Improvement of Biosensors Using Si Ring Optical Resonators, JAPANESE JOURNAL OF APPLIED PHYSICS, 50(4), APR 2011
  26. Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation, FEMS MICROBIOLOGY LETTERS, 320(1), 25-32, JUL 2011
  27. Why does the silica-binding protein Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces", COLLOIDS AND SURFACES B-BIOINTERFACES, 86(2), 359-363, SEP 1 2011
  28. Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase, PROCESS BIOCHEMISTRY, 46(9), 1747-1752, SEP 2011
  29. Application of an enteric coat increases the resistance to gastric juices for swallowable biosensors, SENSORS AND ACTUATORS B-CHEMICAL, 160(1), 379-383, DEC 15 2011
  30. Evaluation of Sensitivity of Fluorescence-Based Asbestos Detection by Correlative Microscopy, JOURNAL OF FLUORESCENCE, 22(1), 357-363, JAN 2012
  31. Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(4), 521-525, APR 2012
  32. Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway, MICROBIAL CELL FACTORIES, 11, SEP 6 2012
  33. Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa, Bioscience= Biotechnology and Biochemistry, 63(1), 155-161, 19990401
  34. Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system, Journal of Bioscience and Bioengineering, 87(3), 273-279, 19990401
  35. Regulation of bacterial phosphate Taxis and polyphosphate accumulation in pesponse to phosphate starvation stress, Journal of Bioscience, 23, 491-499, 19980401
  36. Isolation and characterization of two cryptic plasmids in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, JOURNAL OF BACTERIOLOGY, 181, 3375-3381, 19990401
  37. Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewates, Progress in Molecular and Subcellular biology, 23, 299-311, 19990401
  38. Software in microorganizms., 52(9), 11-16, 19940401
  39. Cloning of the Pseudomonas awruginosa gene encoding CDP-diglyceride synthetase, Gene, 172(1), 165-166, 19960401
  40. Molecular analysis of the phosphate sensing in Pseudomonas aeruginosa, Beijerinck Centennial Microbial physiology and gene regulation. Delf University Press, 181-182, 19950401
  41. Genetic engineering of polyphosphate accumulation in bacteria, Beijerinck Centennial Microbial physiology and gene regulation. Delf University Press, 233-234, 19950401
  42. Isolation and characterization of Enterobacter cloacae mutants which are defective in chemotaxis toward in organic phosphate, Journal of Bacriology, 179(19), 6192, 19970401
  43. Molecular Breeding of Microbes and Environmental Biotechnology, Microbes and Environmants, 13(2), 95, 19980401
  44. Molecular analysis of the phosphate-specific transport(pst)operon of Pseudomonas aeruginosa, Molecular and General Genetics, 250(6), 692-698, 19960401
  45. Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene, J. Gen. Microbiol.=, 136, 2209-2216, 19900401
  46. Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation, Journal of General Microbiology(U. K. )=, 136, 1223-1230, 19900401
  47. Cloning of the Pseudomonas aeruginosa gene encoding CDP-diglyceride synthetase, Gene, 172, 165-166, 19960401
  48. Bacterial phosphonate degradation, phosphite oxidation and polyphosphate accumulation, Resources= Conservation and Recycling, 18, 125-134, 19960401
  49. Chemotactic signal transduction network in Pseudomonas aeruginosa, American Society for Microbiology, 188-194, 19960401
  50. Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa, Microbiology, 143, 3223-3229, 19970401
  51. Development of a genetic system for an algal-lytic bacterium, Appl. Environ. Microbiol. =, 64, 2061-2064, 19980401
  52. Enhanced utilization of phosphonate and phosphite by Klebsiella aerogenes, Appl. Environ. Microbiol. =, 64, 3754-3758, 19980401
  53. The synthesis of optically pure enantiomers of N- acyl homoserine lactone autoinducers and their analogues, Chem. Lett., 314-315, 20010201
  54. Isolation and characterization of the Enterobacter cloacae cheR mutant defective in phosphate taxis, Biosci. Biotechnol. Biochem., 65(2), 456-458, 20010201
  55. Role of inorganic polyphosphate in promoting ribosomal protein degradation by the Lon protease in E. coli, Science, 293, 705-708, 20010701
  56. Development of rapid toxicity assays by using motile bacteria labeled with the green fluorescent protein (GFP) as a biosensor, Proceedings of 5th International Symposium on Environmental Biotechmology, 634-637, 20010701
  57. Release of polyphosphate granules from activated sludge by heat treatment and its application for phosphorus reuse and recycling, Proceedings of 5th International Symposium on Environmental Biotechmology, 23-26, 20010701
  58. Quorum sensing in gram-negative bacteria using synthetic autoinducers and thei analogues, Proceedings of 5th International Symposium on Environmental Biotechmology, 1037-1040, 20010701
  59. Polyphosphate overplus in Klebsiella aerogenes, Proceedings of 5th International Symposium on Environmental Biotechmology, 55-58, 20010701
  60. Purification and characterization of an algicidal extracellular protease of the marine bacterium Pseudoalteromonas sp. strain A28, Proceedings of 5th International Symposium on Environmental Biotechmology, 59-62, 20010701
  61. Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, J. Bacteriol., 182(3), 825-828, 20000201
  62. Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS, J. Biosci. Bioeng., 90(6), 688-690, 20000601
  63. Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa, J. Bacteriol., 182(12), 3400-3404, 20000601
  64. Development of a genetic transformation system for an alga-lysing bacterium, Appl. Environ. Microbiol. =, 64(6), 2061-2064, 19980601
  65. Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp.Strain ENI-11, J. Bacteriol., 182, 825-828, 20000401
  66. Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, Biosc. Biochem. Biotechnol., 64, 1754-1757, 20000401
  67. Involvement of an extracellular protease in the algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28, Appl. Environ. Microbiol. =, 66, 4334-4339, 20000401
  68. Molecular analysis of polyphosphate accumulation in bacteria, Biochemistry (Moscow), 65, 362-367, 20000401
  69. The optical resolution of synthetic N-acyl-homoserine lactone autoinducers and their analogues, Chem. Lett., 314-315, 20010401
  70. Inorganic polyphosphate: a molecular tag to identify ribosomal proteins for degradation by Lon protease in E. coli, Science, 293, 705-708, 20010401
  71. A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP generation and bioluminescence reactions, Biochemical Engineering Journal, 9, 193-197, 20010401
  72. Chemotaxis of the Nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride, J. Biosci. Bioeng., 91, 322-324, 20010401
  73. Development of rapid toxicity assays by using motile bacteria labeled with the green fluorescent protein (GFP) as a biosensor., Proceedings of 5th International Symposium on Environmental Biotechmology, 634-637, 20010701
  74. Release of polyphosphate granules from activated sludge by heat treatment and its application for phosphorus reuse and recycling., Proceedings of 5th International Symposium on Environmental Biotechmology, 23-26, 20010701
  75. Quorum sensing in gram-negative bacteria using synthetic autoinducers and thei analogues., Proceedings of 5th International Symposium on Environmental Biotechmology, 1037-1040, 20010701
  76. Polyphosphate overplus in Klebsiella aerogenes, Proceedings of 5th International Symposium on Environmental Biotechmology, 55-58, 20010701
  77. Purification and characterization of polyphosphate-dependent glucokinase of Microlunatus phosphovorus, Proceedings of 5th International Symposium on Environmental Biotechmology, 1061-1064, 20010701
  78. Molecular analysis of the nitrification henes in Nitrosomonas sp. strain ENI-11, Proceedings of 5th International Symposium on Environmental Biotechmology, 67-70, 20010701
  79. Purification and characterization of an algicidal extracellular protease of the marine bacterium Pseudoalteromonas sp. strain A28, Proceedings of 5th International Symposium on Environmental Biotechmology, 59-62, 20010701
  80. Bacterial sensing system as a potential tool for rapid toxicity assays, Core Research for Environmental Science and Technology International Aquatic Environment Workshop, 103-106, 20000201
  81. Inorganic polyphosphate kinase is required to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli, Proc. Natl. Acad. Sci. USA, 96, 14264-14269, 19990401
  82. Polyphosphate kinase as a nucleotide diphosphate kinase in Escherichia coli and Pseudomonase aeruginosa, Proc. Natl. Acad. Sci. USA, 94, 439-442, 19970401
  83. Guanosine tetra- and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coli, J. Biol. Chem., 272, 21240-21243, 19970401
  84. Molecular cloning of a CDP-diacylglycerol synthase gene of Pseudomonas aeruginosa, Gene, 172, 165-166, 19960401
  85. Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa, J. Bacteriol., 177, 7019-7025, 19950401
  86. Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis, J. Bacteriol., 177, 5582-5589, 19950401
  87. Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autoysin operon of Bacillus subtilis, J. Bacteriol., 176, 5177-5180, 19940401
  88. Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis, J. Bacteriol., 175, 6260-6268, 19930401
  89. Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis, Mol. Gen. Genet., 241, 380-388, 19930401
  90. Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility, FEMS Microbiol. Letters, 112, 135-140, 19930401
  91. High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation, J. Bacteriol., 175, 795-801, 19930401
  92. Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases, FEMS Microbiol. Letters, 95, 109-114, 19920401
  93. Molecular cloning and sequencing of the upstream region of the major Bacillus subtilis autolysin gene: a modifier protein exhibiting sequence homology to the major autolysin and the spoIID product, J. Gen. Microbiol.=, 138, 1067-1076, 19920401
  94. Genetic structure, isolation and characterization of a Bacillus licheniformis cell wall hydrolase, Mol. Gen. Genet., 234, 129-137, 19920401
  95. Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene, J. Bacteriol., 173, 7304-7312, 19910401
  96. Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis, FEMS Microbiol. Letters, 81, 9-14, 19910401
  97. Precipitation Behavior of 1,1,di(p-Hydroxyphenyl)-Cyclohexane Clathrate Crystals From Acetone Solutions Containing d-Limonene, J. Inclusion Phenomena, 10, 305-312, 19910401
  98. An Approach to Molecular Artificial Life: bacterial intelligent behavior and its computer model, Artificial Life V (C. G. Langton and K. Shimohara= Ed.), 395-401, 19960401
  99. Behavioral responses of the ciliated protozoan Paramecium caudatum to 2,4-dichlorophenoxyacetic acid and its analogues, J. Biosci. Bioeng., 93, 416-420, 20020401
  100. Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp. sain PCC6803, Appl. Environ. Microbiol., 68, 4107-4110, 20020401
  101. Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms, Jouranal of Biotechnology, 101(1), 11-18, 20030101
  102. A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering, 95(6), 637-640, 20030601
  103. Pilot plant tests on the novel process for phosphorus recovery from municipal wastewater, Journal of Chemical Engineering of Japan, 36(10), 1143-1146, 20031001
  104. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli, J. Biol. Chem., 279, 34406-34410, 20040401
  105. Mutants of the nematode Caenorhabditis elegans that are defective specifically in their attraction to cycloheximide, J. Biosci. Bioeng., 96(2), 149-153, 20030801
  106. Strictly polyphosphate-dependent glucokinase in a polyphosphate accumulating bacterium, Microlunatus phosphovorus, J. Bacteriol., 185(18), 5654-5656, 20030401
  107. Chemotaxis proteins and transducers for aerotaxis in Psudomonas aeruginosa., FEMS Microbiol. Letters, 231, 247-252, 20040401
  108. The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa, J. Biosci. Bioeng., 97(3), 184-190, 20040301
  109. Characterization of pEC01, a novel plasmid required for phosphate taxis in Enterobacter cloacae IFO 3320, Microbs Environ., 19(1), 45-52, 20040401
  110. A laboratory-scale test of anaerobic digestion and methane production after phosphorus recovery from waste activated sludge, J. Biosci. Bioeng., 97(6), 365-368, 20040601
  111. Chemotaxis proteins and transducers fro aerotaxis in Pseudomonas putida, FEMS Microbiol. Lett., 231, 247-252, 20040401
  112. Expression of Pseudomonas aeruginosa aer-2, one of two aerotaxis transducer genes, is controlled by RpoS, J. Bacteriol. (in press), 20050401
  113. Chemotaxis as sensing system for environmental pollutions, The 10th APCChE Congress proceedings, 2J-05, 20040401
  114. Biotransformation of toluene to o-cresol in a two-phase system by a solvent-tolerant bacterium, The 10th APCChE Congress proceedings, 3L-11, 20040401
  115. Role of the maxillary palpus in the feeding preference behabior of the silk worm, Bombyx mori, The 10th APCChE Congress proceedings, 3P-01-050, 20040401
  116. Control of quorum sensing systems in Pseudomonas aeruginosa by acyl homoserine lactone analogues, The 10th APCChE Congress proceedings, 3P-01-053, 20040401
  117. Characterization of Rhodococcus opacus B-4, a strain able to grow with benzene in a two-pase (organic-aqueous) medium, The 10th APCChE Congress proceedings, 3P-01-084, 20040401
  118. Improvement of phosphate recovery from wastewater by heatphos process, P1-018(ENV), 20050501
  119. Sommelier mice: Biosensor for odor discrimination among liquors, P3-035(CUR), 20050501
  120. Analysis of the activities in the medaka fish olfactory bulb by optical imaging, AROMA RESEARCH, 23(6), 276-282, 20050801
  121. Learning experience enables mice to discrimiinate odors among liquors, FFI Journal, 210(8), 765-769, 20050801
  122. Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28, Bioscicence Biotechnology and Biochemistry, 66, 1366-1369, 20020601
  123. The effects of cyclodextrins on autoinducer activities of quorum sensing in Pseudomonas aeruginosa, Journal of Inclusion Phenomena and Macrocyclic Chemistry, 44, 381-382, 20021201
  124. Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase / oxygenase large and small subunits in Nitrosomonas sp. strain ENI-11, Bioscicence Biotechnology and Biochemistry, 66, 632-635, 20020301
  125. Development of novel biological process for recovering phosphorus from wastewater, Journal of Chemical Industry and Engineering (China), 53, SUPPL 21-22, 20021101
  126. Development of a genetic transformation system for Benzene-tolerant Rhodococcis opacus strains, Journal of Bioscience and Bioengineering, 99, 408-414, 20050425
  127. Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system, Journal of Industrial Microbiology and Biotechnology, 32, 542-547, 20051201
  128. Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitorosomonas sp. Strain ENI-11, Bioscicence Biotechnology and Biochemistry, 70(8), 1875-1881, 20060801
  129. Identification and chracterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene, The Journal of Bacteriology, 188(18), 6700-6702, 20060901
  130. Inorganic polyphosphate stimulates Lon-mediated proteolysis of nucleoid proteins in Escherichia coli, Cellular and Molecular Biology, 52(4), 23-29, 20061231
  131. Polyamines affect polyphosphate accumulation in Escherichia coli, Journa of Environmental Biotechnology, 6, 41-46, 20060601
  132. Polyphosphate-ATP amplification technology: Principle and its application to specific bacteria detection, Journa of Environmental Biotechnology, 6, 105-108, 20060601
  133. Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection, J. Bioscience and Bioengineering, 104, 55-61, 20070725
  134. Effect of mineral elements on phosphorus release from heated sewage sludge, Bioresource Technology, 98, 2533-2537, 20070901
  135. Transcriptional Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11, Biosci. Biotechnol. Biochem., 1875-1881, 20070823
  136. Identification of Chemosensory Proteins for Trichloroethylene in Pseudomonas aeruginosa, Journal of bioscience and bioengeering, 99(4), 396-402, 20050425
  137. Isolation and Characterization of Benzene-Tolerant Rhodococcus opacus stains, Journal of Bioscience and Bioengineering, 99(4), 378-382, 20050425
  138. Ethylene Chemotaxis in Pseudomonas aeruginosa and Other Pseudomoonas Species, Microbes and Environments, 22(2), 186-189, 20070601
  139. Bioproduction of 3-Methlcatechol from Toluene in a Two-Phase(organic-Aqueous)System by a Genetically Modified solvent-Tolerant Pseudomonas putida Strain T-57, Journal of Environmental Biotechnology, 7(1), 39-44, 20070601
  140. Protein engineering of penicillinase as affinity ligands for bioprocessing, Journal of Fermentation and Bioengineering, 67, 315-320, 19890101
  141. Precipitation Behavior of 1,1,di(p-Hydroxyphenyl)-Cyclohexane Clathrate Crystals From Acetone Solutions Containing d-Limonene, J. Inclusion Phenomena, 10, 305-312, 19910401
  142. The role of attention in mice olfactory response, Proceedings of Asia-Pacific Biochemical Engineering Conference 2007, P1-41, 20071105
  143. Development of genome engineering technology for solvent-tolerant Rhodococcus opacus strain B4, Proceedings of Asia-Pacific Biochemical Engineering Conference 2007, P3-10, 20071107
  144. High Sensitivity Detection of ATP using Bioluminescence at an Optical Fiber End, Trans. IEEE of Japan, 10(127), 1498-1503, 20071001
  145. Bioluminescent Reaction by Immobilized Luciferase, IEEJ Trans. EIS, 131(1), 23-28, 20110101
  146. Detection of living Salmonella cells using bioluminescence, Biotechnology Letters., 31(5), 737-741, 20090501
  147. High-sensitivity detection of ATP using bioluminescence at an optical fiber end, Electronics and communications in Japan, 92(9), 1498-1503, 20091001
  148. Slot ring biosensors using silicon nitride waveguides with small temperature coefficient, Proc. SPIE, 8431(-), 84311S, 20120601
  149. Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp strain ENI-11, JOURNAL OF BACTERIOLOGY, 182(3), 825-828, FEB 2000
  150. Molecular analysis of polyphosphate accumulation in bacteria, BIOCHEMISTRY-MOSCOW, 65(3), 304-308, MAR 2000
  151. Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa, JOURNAL OF BACTERIOLOGY, 182(12), 3400-3404, JUN 2000
  152. Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64(8), 1754-1757, AUG 2000
  153. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(10), 4334-4339, OCT 2000
  154. Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90(6), 688-690, DEC 2000
  155. Isolation and characterization of the Enterobacter cloacae cheR mutant defective in phosphate taxis, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65(2), 456-458, FEB 2001
  156. The synthesis of optically pure enantiomers of N-acyl-homoserine lactone autoinducers and their analogues, CHEMISTRY LETTERS, 314-315, APR 5 2001
  157. Chemotaxis of the nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(3), 322-324, MAR 2001
  158. A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP regeneration and bioluminescence reactions, BIOCHEMICAL ENGINEERING JOURNAL, 9(3), 193-197, DEC 20 2001
  159. Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66(3), 632-635, MAR 2002
  160. A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling, BIOTECHNOLOGY AND BIOENGINEERING, 78(3), 333-338, MAY 5 2002
  161. Behavioral responses of the ciliated protozoan Paramecium caudatum to 2,4-dichlorophenoxyacetic acid and its analogues, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93(4), 416-420, APR 2002
  162. Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp strain A28, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66(6), 1366-1369, JUN 2002
  163. Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp strain PCC6803, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(8), 4107-4110, AUG 2002
  164. Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms, JOURNAL OF BIOTECHNOLOGY, 101(1), 11-18, FEB 27 2003
  165. The effects of cyclodextrins on autoinducer activities of quorum sensing in Pseudomonas aeruginosa, JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY, 44(1-4), 381-382, DEC 2002
  166. Molecular mechanism of polyphosphate accumulation in bacteria and its application - Monograph, SEIBUTSU-KOGAKU KAISHI, 81(3), 104-111, 2003
  167. A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95(6), 637-640, JUN 2003
  168. Strictly polyphosphate-dependent glucokinase in a polyphosphate-accumulating bacterium, Microlunatus phosphovorus, JOURNAL OF BACTERIOLOGY, 185(18), 5654-5656, SEP 2003
  169. Mutants of the nematode Caenorhabditis elegans that are defective specifically in their attraction to cycloheximide, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 96(2), 149-153, AUG 2003
  170. Pilot plant tests on the novel process for phosphorus recovery from municipal wastewater, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 36(10), 1143-1146, OCT 2003
  171. ATP amplification for ultrasensitive bioluminescence assay: Detection of a single bacterial cell, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68(6), 1216-1220, JUN 2004
  172. Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa, FEMS MICROBIOLOGY LETTERS, 231(2), 247-252, FEB 16 2004
  173. The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(3), 184-190, MAR 2004
  174. New basic and applied aspects of polyphosphate in microorganisms, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 78(8), 738-743, 2004
  175. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(33), 34406-34410, AUG 13 2004
  176. A laboratory-scale test of anaerobic digestion and methane production after phosphorus recovery from waste activated sludge, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(6), 365-368, JUN 2004
  177. Expression of Pseudomonas aeruginosa aer-2, one of two aerotaxis transducer genes, is controlled by RpoS, JOURNAL OF BACTERIOLOGY, 187(4), 1533-1535, FEB 2005
  178. Isolation and characterization of benzene-tolerant Rhodococcus opacus strains, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 378-382, APR 2005
  179. Identification of chemosensory proteins for trichloroethylene in Pseudomonas aeruginosa, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 396-402, APR 2005
  180. Development of a genetic transformation system for benzene-tolerant Rhodococcus opacus strains, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 408-414, APR 2005
  181. Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 32(11-12), 542-547, DEC 2005
  182. A polyphosphate-Lon protease complex in the adaptation of Escherichia coli to amino acid starvation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70(2), 325-331, FEB 2006
  183. Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70(8), 1875-1881, AUG 2006
  184. Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene, JOURNAL OF BACTERIOLOGY, 188(18), 6700-6702, SEP 2006
  185. The Si-Tag for immobilizing proteins on a silica surface, BIOTECHNOLOGY AND BIOENGINEERING, 96(6), 1023-1029, APR 15 2007
  186. Inorganic polyphosphate stimulates Lon-mediated proteolysis of nucleoid proteins in Escherichia coli, CELLULAR AND MOLECULAR BIOLOGY, 52(4), 22-29, 2006
  187. Effect of mineral elements on phosphorus release from heated sewage sludge, BIORESOURCE TECHNOLOGY, 98(13), 2533-2537, SEP 2007
  188. Increase in bioluminescence intensity of firefly luciferase using genetic modification, ANALYTICAL BIOCHEMISTRY, 366(2), 131-136, JUL 15 2007
  189. Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(1), 55-61, JUL 2007
  190. Performance of mice in discrimination of liquor odors: Behavioral evidence for olfactory attention, CHEMICAL SENSES, 33(3), 283-290, MAR 2008
  191. Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(17), 5676-5678, SEP 2007
  192. Ethylene chemotaxis in Pseudomonas aeruginosa and other Pseudomonas species, MICROBES AND ENVIRONMENTS, 22(2), 186-189, 2007
  193. Enhanced dibenzothiophene biodesulfurization in a microchannel reactor, BIOTECHNOLOGY LETTERS, 30(3), 451-454, MAR 2008
  194. Single bacterial cell detection using a mutant luciferase, BIOTECHNOLOGY LETTERS, 30(6), 1051-1054, JUN 2008
  195. Continuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay, ANALYTICAL BIOCHEMISTRY, 379(1), 116-120, AUG 1 2008
  196. Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(1), 1-7, JUL 2008
  197. Detection of living Salmonella cells using bioluminescence, BIOTECHNOLOGY LETTERS, 31(5), 737-741, MAY 2009
  198. Si Ring Optical Resonators for Integrated On-Chip Biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 48(4), APR 2009
  199. High-Sensitivity Detection of ATP Using Bioluminescence at an Optical Fiber End, ELECTRONICS AND COMMUNICATIONS IN JAPAN, 92(9), 53-59, SEP 2009
  200. The Silicon Layer Supports Acid Resistance of Bacillus cereus Spores, JOURNAL OF BACTERIOLOGY, 192(1), 111-116, JAN 2010
  201. Stable polyphosphate accumulation by a pseudo-revertant of an Escherichia coli phoU mutant, BIOTECHNOLOGY LETTERS, 35(5), 695-701, MAY 2013
  202. Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(12), 5677-5684, JUN 2014
  203. Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts, JOURNAL OF BIOTECHNOLOGY, 182, 68-73, JUL 20 2014
  204. Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization, ANALYTICAL METHODS, 6(16), 6232-6235, AUG 21 2014
  205. Development of an automated asbestos counting software based on fluorescence microscopy, ENVIRONMENTAL MONITORING AND ASSESSMENT, 187(1), JAN 2015
  206. A BRET-Based Homogeneous Insulin Assay Using Interacting Domains in the Primary Binding Site of the Insulin Receptor, ANALYTICAL CHEMISTRY, 87(5), 2764-2770, MAR 3 2015
  207. A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA, ANALYST, 140(4), 999-1003, 2015
  208. A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids, BIOSENSORS & BIOELECTRONICS, 74, 222-226, DEC 15 2015
  209. Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin, RSC ADVANCES, 5(127), 105279-105287, 2015
  210. The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat, JOURNAL OF BACTERIOLOGY, 198(2), 276-282, JAN 2016
  211. Detection of antibody-antigen reaction by silicon nitride slot-ring biosensors using protein G, OPTICS COMMUNICATIONS, 365, 16-23, APR 15 2016
  212. Surface Modification of Cell Scaffold in Aqueous Solution Using TiO2 Photocatalysis and Linker Protein L2 for Patterning Primary Neurons, E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY, 13, 213-218, 2015
  213. Differential Si ring resonators for label-free biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 55(4), APR 2016
  214. A Lactobacillus mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5), 955-961, MAY 2016

Publications such as books

  1. 2000/00, Molecular genetics of bacterial polyphosphate accumulation to better understand the mechanism underlying biological phosphorus removal. , 2000, 00, 15
  2. 2003/00, Applications of polyphosphate and other biopolmers in wastewater treatment. , 2003, 00, 18
  3. 1999/02, New current of biotechnology , 1999, 02, 4254171064, 156
  4. 1996, Chemotactic signal transduction network in Pseudomonas aeruginosa , Molecular Biology of Pseudomonads. American Society for Microbiology= Washington USA, 1996
  5. 1999, Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater , Inorganic polyphosphates, 1999
  6. 1999, Progress in Molecular and Subcellar Biology, --Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater , Springer-Verlag, 1999
  7. 2003, Applications of polyphosphate and other biopolmers in wastewater treatment , Wiley-VCHA= "Biopolymers: Vol.10 General Aspects and Special "Biopolymers: Vol.10 General Aspects and Special Biopolymers: Vol.10 General Aspects and Special Applications (A. Steinbuchel [ed.]), 2003, 18
  8. 1997, An Approach to Molecular Artificial Life: bacterial intelligent behavior and its computer modelin Artificial Life V (C. G. Langton and K. Shimohara, Ed.), Artificial Life V, 1997, 7
  9. 2004/11, Basic molecular biology for biotechnology , 2004, 11, 4759809686, 199
  10. 2008/08, ATP regeneration and biological production of phosphorylated compounds using thermostable polyphosphate kinase, 2008, 08, 8
  11. 2012/02, FIBER OPTIC SENSOR Chapter 19: High-sensitivity Detection of Bioluminescnece at an Optical Fiber End for an ATP sensor, 2012, 02, 9789533079226, 518

Awards

  1. 1995年10月, "Human frontier science program, Long-term fellowship award."