小松澤 均Hitoshi Komatsuzawa

Last Updated :2019/12/02

所属・職名
大学院医系科学研究科(歯)(歯学)細菌学教授
メールアドレス
komatsuzhiroshima-u.ac.jp
自己紹介
細菌学は口腔および全身の感染症を理解する上で基本となる学問です。 細菌研究においては①薬剤耐性菌の病原性・薬剤耐性機序の解明および疫学研究、②う蝕・歯周病原因菌の病原性・生体定着機構の解明、③口腔マイクロバイオーム(細菌叢)形成機序の解明を中心に日々、研究を進めております。

基本情報

主な職歴

  • 1992年10月01日, 2000年05月31日, 広島大学, 歯学部, 助手
  • 2000年06月01日, 2002年03月31日, 広島大学, 歯学部, 助教授
  • 2002年04月01日, 2008年02月29日, 広島大学, 大学院医歯薬学総合研究科, 助教授
  • 2008年03月01日, 2019年04月30日, 鹿児島大学, 教授
  • 2019年05月01日, 広島大学, 大学院医系科学研究科, 教授

学位

  • 博士(歯学)(広島大学)

教育担当

  • 【学士課程】 歯学部 : 歯学科
  • 【博士課程前期】 医系科学研究科 : 総合健康科学専攻
  • 【博士課程】 医系科学研究科 : 医歯薬学専攻

教育活動

授業担当

  1. 2019年, 学部専門, 2ターム, 微生物学I
  2. 2019年, 学部専門, 4ターム, 微生物学II
  3. 2019年, 学部専門, セメスター(前期), 口腔微生物学・免疫学実習
  4. 2019年, 学部専門, 1ターム, 歯学研究特論I
  5. 2019年, 学部専門, セメスター(前期), 微生物学・口腔微生物学
  6. 2019年, 学部専門, 2ターム, 基礎オーラルサイエンス実習
  7. 2019年, 学部専門, セメスター(前期), 微生物学・口腔微生物学
  8. 2019年, 修士課程・博士課程前期, セメスター(後期), 特別演習
  9. 2019年, 修士課程・博士課程前期, セメスター(前期), 特別研究
  10. 2019年, 博士課程・博士課程後期, セメスター(前期), 細菌学特別演習
  11. 2019年, 博士課程・博士課程後期, セメスター(前期), 細菌学特別実験
  12. 2019年, 修士課程・博士課程前期, セメスター(前期), 特別演習
  13. 2019年, 修士課程・博士課程前期, セメスター(後期), 特別研究
  14. 2019年, 博士課程・博士課程後期, セメスター(前期), 特別演習
  15. 2019年, 博士課程・博士課程後期, セメスター(後期), 特別演習
  16. 2019年, 博士課程・博士課程後期, セメスター(前期), 特別研究
  17. 2019年, 博士課程・博士課程後期, セメスター(後期), 特別研究
  18. 2019年, 修士課程・博士課程前期, 3ターム, 生体防御学
  19. 2019年, 博士課程・博士課程後期, セメスター(前期), 細菌学特別演習
  20. 2019年, 博士課程・博士課程後期, セメスター(後期), 細菌学特別演習
  21. 2019年, 博士課程・博士課程後期, セメスター(前期), 細菌学特別研究
  22. 2019年, 博士課程・博士課程後期, セメスター(後期), 細菌学特別研究

研究活動

学術論文(★は代表的な論文)

  1. ★, Single mutations in BraRS confer high resistance against nisin A in Staphylococcus aureus., MicrobiologyOpen, 17巻, pp. e791, 2019/1/17
  2. Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans., Microbiology and Immunology, 63巻, 7号, pp. 251-260
  3. Heparin-LL37 complexes are less cytotoxic for human dental pulp cells and have undiminished antimicrobial and LPS-neutralizing abilities., Int Endod J, 52巻, 9号, pp. 1327-1343
  4. Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans., DNA Res., 25巻, 2号, pp. 207-215
  5. Impact of a 7-Day field training on oral health condition in Japan ground self-defense force personnel., Mil Med., 182巻, 7号, pp. e1869-e1877
  6. Recombinant Sox enzymes from Paracoccus pantotrophus degrade hydrogen sulfide, a major component of oral malodor., Microbes Environ., 32巻, 1号, pp. 54-60
  7. Significant increase of oral bacteria in the early pregnancy period in Japanese women. J Investig, Clin Dent., 8巻, pp. e12189
  8. Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus., PLoS One., 11巻, 11号, pp. e0165831
  9. Lysine and threonine biosynthesis from aspartate contributes to Staphylococcus aureus growth in calf serum., Appl Environ Microbiol, 82巻, 20号, pp. 6150-6157
  10. ★, Two-component systems involved in susceptibility to nisin A in Streptococcus pyogenes. 82(19):5930-5939, 2016, Appl Environ Microbiol., 82巻, 19号, pp. 5930-5939
  11. Staphylococcus aureus SrrAB affects susceptibility to hydrogen peroxide and co-existence with Streptococcus sanguinis., PLoS One, 11巻, 7号, pp. e0159768
  12. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/FAK cascade., Cell Microbiol., 18巻, 12号, pp. 1723-1738
  13. C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains. 2016 Mar;60(3):139-147, 2016, Microbiol Immunol., 60巻, 3号, pp. 139-147
  14. LL37 induces VEGF expression in dental pulp cells through ERK signalling. 48(7):673-679, Int Endod J., 48巻, 7号, pp. 673-679
  15. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect., Peptides, 46巻, pp. 136-142
  16. Three distinct two-component systems are involved in resistance to the class I bacteriocins, Nukacin ISK-1 and nisin A, in Staphylococcus aureus., PLoS One, 22巻, 8号, pp. e69455
  17. Involvement of the novel two-component NsrRS and LcrRS systems in distinct resistance pathways against nisin A and nukacin ISK-1 in Streptococcus mutans., Appl Environ Microbiol., 79巻, 15号, pp. 4751-4755
  18. ★, Staphylococcus aureus SasA is responsible for binding to salivary agglutinin, gp340, derived from human saliva., Infect Immun., 81巻, 6号, pp. 1870-1879
  19. ★, The S-layer of Tannerella forsythia contributes to serum resistance and oral bacterial co-aggregation., Infect Immun., 81巻, 4号, pp. 1198-1206
  20. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells., J Periodontal Res., 48巻, 2号, pp. 228-234
  21. ★, dpr and sod in Streptococcus mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H2O2., Appl Environ Microbiol., 79巻, 5号, pp. 1436-1443
  22. ★, GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence., PLoS One, 7巻, 3号, pp. e33382
  23. ★, Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms. 27(2):124-135, 2012, Mol Oral Microbiol., 27巻, 2号, pp. 124-135
  24. ★, Growth-phase dependence of susceptibility to antimicrobial peptides in Staphylococcus aureus, , Microbiology, 157巻, pp. 1786-1797
  25. Lipopolysaccharides mediate leukotoxin secretion in Aggregatibacter actinomycetemcomitans., Mol Oral Microbiol., 27巻, 2号, pp. 70-82
  26. Detection of Fusobacterium nucleatum in chorionic tissues of high-risk pregnant women., J Clin Periodontol., 39巻, 5号, pp. 417-424
  27. ★, Expressions of virulence factors in Staphylococcus aureus grown in serum., Appl Environ Microbiol., 77巻, 22号, pp. 8097-8105
  28. ★, Bacitracin sensing and resistance in Staphylococcus aureus., FEMS Microbiol Lett., 320巻, 1号, pp. 33-39
  29. MK615: a new therapeutic approach for the treatment of oral disease., Med Hypotheses., 77巻, 2号, pp. 258-260
  30. Aggregatibacter actinomycetemcomitans Omp29 is associated with bacterial entry to gingival epithelial cells by F-actin rearrangement., PLoS One, 29巻, 6号, pp. e18287
  31. Regulatory mechanism for exfoliative toxin production in Staphylococcus aureus. 79(4):1660-1670, 2011, Infect Immun., 79巻, 4号, pp. 1660-1670
  32. Additive effects of orexin B and vasoactive intestinal polypeptide on LL-37-mediated antimicrobial activities. 233(1-2):37-45, 2011, J Neuroimmunol., 233巻, 1-2号, pp. 37-45
  33. Distinct two-component systems in methicillin-resistant Staphylococcus aureus can change the susceptibility to antimicrobial agents., J Antimicrob Chemother., 65巻, 7号, pp. 1536-1537
  34. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics. 36(6):1009-1013, 2010, J Endod., 36巻, 6号, pp. 1009-1013
  35. Automutanolysin disrupts clinical isolates of cariogenic streptococci in biofilms and planktonic cells., Oral Microbiol Immunol., 24巻, pp. 451-455
  36. ★, Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus., J Antimicrob Chemother., 61巻, 6号, pp. 1266-1269
  37. Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells., Infect Immun., 76巻, pp. 4783-4791
  38. Irsogladine maleate abolishes the increase in interleukin-8 levels caused by outer membrane protein 29 from Aggregatibacter (Actinobacillus) actinomycetemcomitans through the ERK pathway in human gingival epithelial cells., J Periodontal Res., 43巻, pp. 508-513
  39. First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan. 13(4):276-7, 2007, J Infect Chemother., 13巻, 4号, pp. 276-277
  40. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes., Int. Immunol., 19巻, 9号, pp. 1095-1102
  41. Cross-reactive adaptive immune response to oral commensal bacteria results in an induction of receptor activator of nuclear factor-kappaB ligand (RANKL)-dependent periodontal bone resorption in a mouse model. Oral Microbiol Immunol. (Mol. Oral Microbiol.), Oral Microbiol Immunol. (Mol. Oral Microbiol.), 22巻, 3号, pp. 208-215
  42. Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan., Microbiol Immunol., 51巻, 3号, pp. 271-277
  43. Kawai T: Innate immune peptide LL-37 displays distinct expression pattern from beta-defensins in inflamed gingival tissue., Clin Exp Immunol., 146巻, 2号, pp. 218-225
  44. ★, Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells., Infect Immun., 74巻, 9号, pp. 5211-5220
  45. Identification and molecular characterization of an N-Acetylmuraminidase, Aml, involved in Streptococcus mutans cell separation., Microbiol Immunol., 50巻, 9号, pp. 729-742
  46. Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans., Infect Immun., 74巻, 12号, pp. 7014-7020
  47. Biogenesis of the Actinobacillus actinomycetemcomitans cytolethal distending toxin holotoxin. 74:3480-3487, 2006, Infect Immun., 74巻, pp. 3480-3487
  48. Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus. Mol Microbiol. 58(4):1087-1101, 2005, 58巻, 4号, pp. 1087-1101
  49. Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., J Biol Chem., 28巻, 1号, pp. 549-558
  50. Staphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1., Microb Pathog., 39巻, 5-6号, pp. 171-176
  51. Induction of keratinocyte migration via transactivation of the EGF receptor by the antimicrobial peptide LL37., J Immunol., 175巻, pp. 4662-4668
  52. Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge. 333(2):502-507,2005, Biochem Biophys Res Commun., 333巻, 2号, pp. 502-507
  53. ★, Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, beta-defensins and LL37, produced by human epithelial cells., J Antimicrob Chemother., 55巻, 6号, pp. 888-896
  54. New mechanisms of skin innate immunity: ASK1-mediated keratinocyte differentiation regulates the expression of beta-defensins, LL37, and TLR2., Eur J Immunol., 35巻, 6号, pp. 1886-1895
  55. Mutation analysis of the histidine residues in the glycylglycine endopeptidase ALE-1., J Bacteriol., 187巻, 2号, pp. 480-487
  56. ★, Reduced content of lysyl-phosphatidylglycerol in the cytoplasmic membrane affects susceptibility to moenomycin, as well as vancomycin, gentamicin, and antimicrobial peptides, in Staphylococcus aureus. 48(12):4800-4807,2004, Antimicrob Agents Chemother., 48巻, 12号, pp. 4800-4807
  57. The gate controlling cell wall synthesis in Staphylococcus aureus., Mol Microbiol., 53巻, 4号, pp. 1221-1231
  58. Zymographic characterization of bacteriolytic enzymes produced by oral streptococci., Microbiol Immunol., 48巻, 6号, pp. 465-469
  59. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans., Inflammation., 27巻, 6号, pp. 341-349
  60. ★, Moenomycin-resistance is associated with vancomycin-intermediate susceptibility in Staphylococcus aureus., Microbiol Immunol., 47巻, 12号, pp. 927-935
  61. ★, Outer membrane protein 100, a versatile virulence factor of Actinobacillus actinomycetemcomitans., Mol Microbiol., 50巻, 4号, pp. 1125-1139
  62. Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors., Cell Biol Int., 27巻, 10号, pp. 815-823
  63. An N-terminal segment of the active component of the bacterial genotoxin cytolethal distending toxin B (CDTB) directs CDTB into the nucleus., J Biol Chem., 278巻, 50号, pp. 50671-50681
  64. Macrophage inflammatory protein-3alpha and beta-defensin-2 stimulate dentin sialophosphoprotein gene expression in human pulp cells., Biochem Biophys Res Commun., 306巻, 4号, pp. 867-871
  65. ★, Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes., Infect Immun., 71巻, 7号, pp. 3730-3739
  66. Prevalence of cytolethal distending toxin production in periodontopathogenic bacteria., J Clin Microbiol., 41巻, 4号, pp. 1391-1398
  67. Molecular characterization of an atl null mutant of Staphylococcus aureus., Microbiol Immunol., 46巻, 9号, pp. 601-612

受賞

  1. 2006年, 小林六造記念賞, 日本細菌学会

外部資金

競争的資金等の採択状況

  1. 科学研究費, 口腔内マイクロバイオーム形成機序および宿主免疫に及ぼす影響の解明, 2016年, 2018年
  2. 科学研究費基盤(C), う蝕・歯周病原菌の口腔内定着機構の解明, 2013年, 2015年
  3. 科学研究費挑戦的萌芽研究, 黄色ブドウ球菌の病原性因子発現に影響を及ぼす外来性因子に関する研究, 2007年, 2008年
  4. 科学研究費基盤研究(C), 歯周病原因菌とヒト抗菌ペプチドの相互作用に関する研究, 2007年, 2008年