Hitoshi Komatsuzawa

Last Updated :2020/08/03

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Dentistry & Oral Health Sciences), Professor
E-mail
komatsuzhiroshima-u.ac.jp
Self-introduction
Bacteriology is important for the understanding oral and systemic infectious diseases.Regarding to bacterial research, we are studying 1) Pathogenicity and antibiotic resistance of antibiotic resistant bacteria, 2)Pathogenicity of cariogenic and periodontopathogenic bacteria, 3)Formation of oral microbiome.

Basic Information

Major Professional Backgrounds

  • 1992/10/01, 2000/05/31, Hiroshima University, School of Dentistry, Research Associate
  • 2000/06/01, 2002/03/31, Hiroshima University, School of Dentistry, Associate Professor
  • 2002/04/01, 2008/02/29, Hiroshima University, Graduate School of Biomedical & Health Sciences , Associate Professor
  • 2008/03/01, 2019/04/30, Kagoshima University, Professor
  • 2019/05/01, Hiroshima University, Graduate School of Biomedical & Health Sciences , Professor

Academic Degrees

  • Hiroshima University

Educational Activity

  • 【Bachelor Degree Program】School of Dentistry : Program of Dentistry
  • 【Master's Program】Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences
  • 【Doctoral Program】Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences

Educational Activity

Course in Charge

  1. 2020, Liberal Arts Education Program1, 2Term, General Health and Oral Sciences I
  2. 2020, Undergraduate Education, 2Term, Topics in Denral Research
  3. 2020, Undergraduate Education, 3Term, Microbiology
  4. 2020, Undergraduate Education, 4Term, Microbiology
  5. 2020, Undergraduate Education, 1Term, Topics in Denral Research
  6. 2020, Undergraduate Education, First Semester, Basic technique for microbiology & immunology
  7. 2020, Undergraduate Education, 2Term, Periodontology
  8. 2020, Undergraduate Education, First Semester, Dental Research Practices
  9. 2020, Undergraduate Education, 2Term, Special Subject
  10. 2020, Undergraduate Education, 3Term, Examination and Diagnosis
  11. 2020, Undergraduate Education, 3Term, Disaster Dentistry and Forensic Odontology
  12. 2020, Undergraduate Education, Second Semester, Dental Research Practices
  13. 2020, Undergraduate Education, 1Term, Microbiology and Oral Microbiology
  14. 2020, Undergraduate Education, 3Term, Practice on Basic Dental Sciences
  15. 2020, Undergraduate Education, 3Term, Disaster Dentistry and Forensic Odontology
  16. 2020, Undergraduate Education, 1Term, Microbiology and Oral Microbiology
  17. 2020, Undergraduate Education, 3Term, Disaster Dentistry and Forensic Odontology
  18. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced research on Bacteriology
  19. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced seminar on Bacteriology
  20. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced seminar on Bacteriology
  21. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced research on Bacteriology
  22. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  23. 2020, Graduate Education (Master's Program) , First Semester, Seminar
  24. 2020, Graduate Education (Master's Program) , Second Semester, Seminar
  25. 2020, Graduate Education (Master's Program) , First Semester, Research
  26. 2020, Graduate Education (Master's Program) , Second Semester, Research
  27. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  28. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  29. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  30. 2020, Graduate Education (Master's Program) , 3Term, Seminar on Host Defense
  31. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Bacteriology
  32. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Bacteriology
  33. 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Bacteriology
  34. 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Bacteriology

Research Activities

Academic Papers

  1. Single mutations in BraRS confer high resistance against nisin A in Staphylococcus aureus., MicrobiologyOpen, 17, e791, 2019/1/17
  2. Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans., Microbiology and Immunology, 63(7), 251-260
  3. Heparin-LL37 complexes are less cytotoxic for human dental pulp cells and have undiminished antimicrobial and LPS-neutralizing abilities., Int Endod J, 52(9), 1327-1343
  4. Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans., DNA Res., 25(2), 207-215
  5. Impact of a 7-Day field training on oral health condition in Japan ground self-defense force personnel., Mil Med., 182(7), e1869-e1877
  6. Recombinant Sox enzymes from Paracoccus pantotrophus degrade hydrogen sulfide, a major component of oral malodor., Microbes Environ., 32(1), 54-60
  7. Significant increase of oral bacteria in the early pregnancy period in Japanese women. J Investig, Clin Dent., 8, e12189
  8. Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus., PLoS One., 11(11), e0165831
  9. Lysine and threonine biosynthesis from aspartate contributes to Staphylococcus aureus growth in calf serum., Appl Environ Microbiol, 82(20), 6150-6157
  10. Two-component systems involved in susceptibility to nisin A in Streptococcus pyogenes. 82(19):5930-5939, 2016, Appl Environ Microbiol., 82(19), 5930-5939
  11. Staphylococcus aureus SrrAB affects susceptibility to hydrogen peroxide and co-existence with Streptococcus sanguinis., PLoS One, 11(7), e0159768
  12. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/FAK cascade., Cell Microbiol., 18(12), 1723-1738
  13. C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains. 2016 Mar;60(3):139-147, 2016, Microbiol Immunol., 60(3), 139-147
  14. LL37 induces VEGF expression in dental pulp cells through ERK signalling. 48(7):673-679, Int Endod J., 48(7), 673-679
  15. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect., Peptides, 46, 136-142
  16. Three distinct two-component systems are involved in resistance to the class I bacteriocins, Nukacin ISK-1 and nisin A, in Staphylococcus aureus., PLoS One, 22(8), e69455
  17. Involvement of the novel two-component NsrRS and LcrRS systems in distinct resistance pathways against nisin A and nukacin ISK-1 in Streptococcus mutans., Appl Environ Microbiol., 79(15), 4751-4755
  18. Staphylococcus aureus SasA is responsible for binding to salivary agglutinin, gp340, derived from human saliva., Infect Immun., 81(6), 1870-1879
  19. The S-layer of Tannerella forsythia contributes to serum resistance and oral bacterial co-aggregation., Infect Immun., 81(4), 1198-1206
  20. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells., J Periodontal Res., 48(2), 228-234
  21. dpr and sod in Streptococcus mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H2O2., Appl Environ Microbiol., 79(5), 1436-1443
  22. GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence., PLoS One, 7(3), e33382
  23. Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms. 27(2):124-135, 2012, Mol Oral Microbiol., 27(2), 124-135
  24. Growth-phase dependence of susceptibility to antimicrobial peptides in Staphylococcus aureus, , Microbiology, 157, 1786-1797
  25. Lipopolysaccharides mediate leukotoxin secretion in Aggregatibacter actinomycetemcomitans., Mol Oral Microbiol., 27(2), 70-82
  26. Detection of Fusobacterium nucleatum in chorionic tissues of high-risk pregnant women., J Clin Periodontol., 39(5), 417-424
  27. Expressions of virulence factors in Staphylococcus aureus grown in serum., Appl Environ Microbiol., 77(22), 8097-8105
  28. Bacitracin sensing and resistance in Staphylococcus aureus., FEMS Microbiol Lett., 320(1), 33-39
  29. MK615: a new therapeutic approach for the treatment of oral disease., Med Hypotheses., 77(2), 258-260
  30. Aggregatibacter actinomycetemcomitans Omp29 is associated with bacterial entry to gingival epithelial cells by F-actin rearrangement., PLoS One, 29(6), e18287
  31. Regulatory mechanism for exfoliative toxin production in Staphylococcus aureus. 79(4):1660-1670, 2011, Infect Immun., 79(4), 1660-1670
  32. Additive effects of orexin B and vasoactive intestinal polypeptide on LL-37-mediated antimicrobial activities. 233(1-2):37-45, 2011, J Neuroimmunol., 233(1-2), 37-45
  33. Distinct two-component systems in methicillin-resistant Staphylococcus aureus can change the susceptibility to antimicrobial agents., J Antimicrob Chemother., 65(7), 1536-1537
  34. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics. 36(6):1009-1013, 2010, J Endod., 36(6), 1009-1013
  35. Automutanolysin disrupts clinical isolates of cariogenic streptococci in biofilms and planktonic cells., Oral Microbiol Immunol., 24, 451-455
  36. Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus., J Antimicrob Chemother., 61(6), 1266-1269
  37. Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells., Infect Immun., 76, 4783-4791
  38. Irsogladine maleate abolishes the increase in interleukin-8 levels caused by outer membrane protein 29 from Aggregatibacter (Actinobacillus) actinomycetemcomitans through the ERK pathway in human gingival epithelial cells., J Periodontal Res., 43, 508-513
  39. First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan. 13(4):276-7, 2007, J Infect Chemother., 13(4), 276-277
  40. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes., Int. Immunol., 19(9), 1095-1102
  41. Cross-reactive adaptive immune response to oral commensal bacteria results in an induction of receptor activator of nuclear factor-kappaB ligand (RANKL)-dependent periodontal bone resorption in a mouse model. Oral Microbiol Immunol. (Mol. Oral Microbiol.), Oral Microbiol Immunol. (Mol. Oral Microbiol.), 22(3), 208-215
  42. Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan., Microbiol Immunol., 51(3), 271-277
  43. Kawai T: Innate immune peptide LL-37 displays distinct expression pattern from beta-defensins in inflamed gingival tissue., Clin Exp Immunol., 146(2), 218-225
  44. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells., Infect Immun., 74(9), 5211-5220
  45. Identification and molecular characterization of an N-Acetylmuraminidase, Aml, involved in Streptococcus mutans cell separation., Microbiol Immunol., 50(9), 729-742
  46. Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans., Infect Immun., 74(12), 7014-7020
  47. Biogenesis of the Actinobacillus actinomycetemcomitans cytolethal distending toxin holotoxin. 74:3480-3487, 2006, Infect Immun., 74, 3480-3487
  48. Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus. Mol Microbiol. 58(4):1087-1101, 2005, 58(4), 1087-1101
  49. Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., J Biol Chem., 28(1), 549-558
  50. Staphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1., Microb Pathog., 39(5-6), 171-176
  51. Induction of keratinocyte migration via transactivation of the EGF receptor by the antimicrobial peptide LL37., J Immunol., 175, 4662-4668
  52. Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge. 333(2):502-507,2005, Biochem Biophys Res Commun., 333(2), 502-507
  53. Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, beta-defensins and LL37, produced by human epithelial cells., J Antimicrob Chemother., 55(6), 888-896
  54. New mechanisms of skin innate immunity: ASK1-mediated keratinocyte differentiation regulates the expression of beta-defensins, LL37, and TLR2., Eur J Immunol., 35(6), 1886-1895
  55. Mutation analysis of the histidine residues in the glycylglycine endopeptidase ALE-1., J Bacteriol., 187(2), 480-487
  56. Reduced content of lysyl-phosphatidylglycerol in the cytoplasmic membrane affects susceptibility to moenomycin, as well as vancomycin, gentamicin, and antimicrobial peptides, in Staphylococcus aureus. 48(12):4800-4807,2004, Antimicrob Agents Chemother., 48(12), 4800-4807
  57. The gate controlling cell wall synthesis in Staphylococcus aureus., Mol Microbiol., 53(4), 1221-1231
  58. Zymographic characterization of bacteriolytic enzymes produced by oral streptococci., Microbiol Immunol., 48(6), 465-469
  59. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans., Inflammation., 27(6), 341-349
  60. Moenomycin-resistance is associated with vancomycin-intermediate susceptibility in Staphylococcus aureus., Microbiol Immunol., 47(12), 927-935
  61. Outer membrane protein 100, a versatile virulence factor of Actinobacillus actinomycetemcomitans., Mol Microbiol., 50(4), 1125-1139
  62. Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors., Cell Biol Int., 27(10), 815-823
  63. An N-terminal segment of the active component of the bacterial genotoxin cytolethal distending toxin B (CDTB) directs CDTB into the nucleus., J Biol Chem., 278(50), 50671-50681
  64. Macrophage inflammatory protein-3alpha and beta-defensin-2 stimulate dentin sialophosphoprotein gene expression in human pulp cells., Biochem Biophys Res Commun., 306(4), 867-871
  65. Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes., Infect Immun., 71(7), 3730-3739
  66. Prevalence of cytolethal distending toxin production in periodontopathogenic bacteria., J Clin Microbiol., 41(4), 1391-1398
  67. Molecular characterization of an atl null mutant of Staphylococcus aureus., Microbiol Immunol., 46(9), 601-612

External Funds

Acceptance Results of Competitive Funds

  1. Grants-in-Aid for Scientific Research, 2016, 2018
  2. Grants-in Aid for Scientific research (C), 2013, 2015
  3. 2007, 2008
  4. 2007, 2008