大前 英司Eiji Oomae

Last Updated :2017/11/01

所属・職名
大学院理学研究科 生命理学講座 助教
メールアドレス
ohmaehiroshima-u.ac.jp

基本情報

学位

  • 博士(理学) (広島大学)
  • 修士(農学) (名古屋大学)

研究分野

  • 生物学 / 生物科学 / 生物物理学

研究キーワード

  • 酵素の機能
  • 酵素の構造
  • 高圧力
  • 深海酵素

教育活動

授業担当

  1. 2017年, 学部専門, セメスター(前期), 物理化学演習
  2. 2017年, 学部専門, セメスター(後期), バイオインフォマティクス
  3. 2017年, 修士課程・博士課程前期, セメスター(前期), 分子生物物理学セミナー
  4. 2017年, 修士課程・博士課程前期, セメスター(後期), 分子生物物理学セミナー

研究活動

学術論文(★は代表的な論文)

  1. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp.479-pp.493, MAR 2015
  2. Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119巻, 41号, pp.13002-pp.13008, OCT 15 2015
  3. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp.111-pp.114, MAY 30 2013
  4. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp.701-pp.709, SEP 2013
  5. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp.2782-pp.2794, DEC 2013
  6. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp.511-pp.519, MAR 2012
  7. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp.165-pp.175, MAR 2011
  8. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp.846-pp.855, APR 2010
  9. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp.591-pp.599, APR 2010
  10. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp.67-pp.71, SEP 2009
  11. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp.1115-pp.1121, JUL-AUG 2008
  12. Pressure effects on enzyme functions, J. Biol. Macromol., pp.23-pp.29, 20070701
  13. Discoveries of deep-sea piezophiles, and their pressure adapted enzymes., Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology, 1巻, pp.114-pp.121, 20071001
  14. Proteins, under high-pressure environments -Discoveries of deep-sea piezophiles, and their pressure adapted enzymes-., The proceedings of International Symposium on Extremophiles and Their Applications, pp.148-pp.155, 20071001
  15. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions, J. Biochem. (Tokyo), 137巻, pp.643-pp.672, 20051001
  16. 1000気圧の環境下でもはたらくタンパク質の不思議 深海好圧性微生物の探索とその耐圧酵素, BIONICS, 2巻, 10号, pp.36-pp.41, 20051001
  17. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: Implication of hydrophobic interactions, JOURNAL OF BIOCHEMISTRY, 137巻, 5号, pp.643-pp.652, MAY 2005
  18. Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium \textit{Shewanella violacea} Strain DSS12, Chemistry Letters, 33巻, 7号, pp.798-pp.799, 20040701
  19. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, Nucreic Acids Research, 32巻, 17号, pp.5291-pp.5302, 20041001
  20. ジヒドロ葉酸還元酵素のループ機能解析, 生物物理, 44巻, 2号, pp.70-pp.74, 20040401
  21. 高圧下における酵素科学, 月刊海洋, 36巻, 9号, pp.692-pp.697, 20040901
  22. Mass spectrometry of hydrogen/deuterium exchange of \textit{Escherichia coli} dihydrofolate reductase: Effects of loop mutations, The Journal of Biochemistry, 135巻, pp.487-pp.494, 20040401
  23. Effects of mutation and ligand binding on the compressibility of protein, Progress in Biotechnology, 19巻, pp.63-pp.70, 20020401
  24. Effects of five-tryptophan mutations on structure, stability and function of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 130巻, pp.439-pp.447, 20010401
  25. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, The Journal of Biochemistry, 128巻, pp.21-pp.27, 20000401
  26. High-pressure NMR reveals active-site motion of folate-bound \textit{Escherichia coli} dihydrofolate reductase, Biochemistry, 39巻, pp.12789-pp.12795, 20000401
  27. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, Chemistry Letters, 1999巻, pp.507-pp.508, 19990401
  28. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp.33-pp.41, 19980401
  29. Effects of point mutations at the flexible loop alanine-145 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp.839-pp.846, 19980401
  30. Acetonitrile-protein interactions; amino acid solubility and preferential solvation, Biochimica et Biophysica Acta, 1387巻, pp.195-pp.205, 19980401
  31. Effects of point mutations at the flexible loop glycine-67 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 119巻, pp.703-pp.710, 19960401
  32. Acid and thermal unfolding of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 120巻, pp.946-pp.953, 19960401
  33. A large compressibility change of protein induced by a single amino acid substitution, Protein Science, 5巻, pp.542-pp.545, 19960401
  34. High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase, BIOCHEMISTRY, 39巻, 42号, pp.12789-pp.12795, 2000