Eiji Oomae

Last Updated :2024/04/03

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Assistant Professor
E-mail
ohmaehiroshima-u.ac.jp

Basic Information

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Agriculture, Nagoya University

Research Fields

  • Biology;Biological Science;Biophysics

Research Keywords

  • Function of enzymes
  • Structure of enzymes
  • High hydrostatic pressure
  • Deep-sea enzymes

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, First Semester, Exercises in Physical Chemistry
  2. 2024, Undergraduate Education, 3Term, Bioinformatics
  3. 2024, Graduate Education (Master's Program) , 1Term, Molecular Biophysics
  4. 2024, Graduate Education (Master's Program) , 2Term, Proteomics
  5. 2024, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
  6. 2024, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B

Research Activities

Academic Papers

  1. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784(7-8), 1115-1121, 2008
    Link to Paper Search Results by DOI
  2. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144(1-2), 67-71, 200909
    Link to Paper Search Results by DOI
  3. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804(4), 846-855, 201004
    Link to Paper Search Results by DOI
  4. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147(4), 591-599, 201004
    Link to Paper Search Results by DOI
  5. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15(2), 165-175, 201103
    Link to Paper Search Results by DOI
  6. Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium \textit{Shewanella violacea} Strain DSS12, Chemistry Letters, 33(7), 798-799, 20040701
  7. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, Nucreic Acids Research, 32(17), 5291-5302, 20041001
  8. Effects of mutation and ligand binding on the compressibility of protein, Progress in Biotechnology, 19, 63-70, 20020401
  9. Mass spectrometry of hydrogen/deuterium exchange of \textit{Escherichia coli} dihydrofolate reductase: Effects of loop mutations, The Journal of Biochemistry, 135, 487-494, 20040401
  10. Effects of point mutations at the flexible loop glycine-67 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 119, 703-710, 19960401
  11. Acid and thermal unfolding of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 120, 946-953, 19960401
  12. A large compressibility change of protein induced by a single amino acid substitution, Protein Science, 5, 542-545, 19960401
  13. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123, 33-41, 19980401
  14. Effects of point mutations at the flexible loop alanine-145 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123, 839-846, 19980401
  15. Acetonitrile-protein interactions; amino acid solubility and preferential solvation, Biochimica et Biophysica Acta, 1387, 195-205, 19980401
  16. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, Chemistry Letters, 1999, 507-508, 19990401
  17. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, The Journal of Biochemistry, 128, 21-27, 20000401
  18. Effects of five-tryptophan mutations on structure, stability and function of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 130, 439-447, 20010401
  19. Pressure effects on enzyme functions, J. Biol. Macromol., 23-29, 20070701
  20. Discoveries of deep-sea piezophiles, and their pressure adapted enzymes., Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology, 1, 114-121, 20071001
  21. Proteins, under high-pressure environments -Discoveries of deep-sea piezophiles, and their pressure adapted enzymes-., The proceedings of International Symposium on Extremophiles and Their Applications, 148-155, 20071001
  22. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions, J. Biochem. (Tokyo), 137, 643-672, 20051001
  23. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824(3), 511-519, 201203
    Link to Paper Search Results by DOI
  24. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572, 111-114, 20130530
    Link to Paper Search Results by DOI
  25. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17(5), 701-709, 201309
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  26. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834(12), 2782-2794, 201312
    Link to Paper Search Results by DOI
  27. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19(2), 479-493, 201503
    Link to Paper Search Results by DOI
  28. Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119(41), 13002-13008, 20151015
    Link to Paper Search Results by DOI
  29. High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase, BIOCHEMISTRY, 39(42), 12789-12795, 2000
  30. Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria., Sub-cellular biochemistry, 72, 2015
  31. Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution., Extremophiles : life under extreme conditions, 20(2), 2016
  32. Similar structural stabilities of 3-isopropylmalate dehydrogenases from the obligatory piezophilic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1., Biochimica et biophysica acta, 1866(5-6)
  33. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1., Extremophiles : life under extreme conditions, 21(3), 2017

Publications such as books

  1. Functional, structural, and thermodynamic characteristics of enzymes from deep-sea microorganisms., Microbial Catalysts Volume 1, Nova Science Publisher, 2019, Scholarly Book, Joint work, E. Ohmae, Y. Miyashita, and C. Kato