Keiko Mitsunaga-Nakatsubo
Last Updated :2023/12/04
- Affiliations, Positions
- Graduate School of Integrated Sciences for Life, Assistant Professor
- E-mail
- kmntn
hiroshima-u.ac.jp
- Self-introduction
- A multicellular organism develops from a single cell-a fertilized egg. During animal development, cells that divide repeatedly change their position and shape, and adhere to form an individual body plan. This process is mediated by multiple extracellular matrix components. To clarify the mechanism and evolution of animal body plan, I am investigating the function of novel extracellular matrix in morphogenesis and its conservation and diversity. I would like to contribute to the elucidation of the pathogenic mechanism of diseases that result from the disruption of the extracellular matrix environment. I am also a member of the committees on gender equality at a university and academic societies.
Basic Information
Academic Degrees
- Dr. of Science, Waseda University
- Master of Science, Waseda University
Educational Activity
- [Bachelor Degree Program] School of Science : Biological Sciences : Biology
- [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
- [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
In Charge of Primary Major Programs
Research Fields
- Biology;Basic biology;Morphology / Structure
- Biology;Biological Science;Developmental biology
Research Keywords
- development
- cell differentiation
- cell fate
- extracellular matrix
- morphogenesis
Affiliated Academic Societies
- the Zoological Society of Japan
- The Japanese Biochemical Society
- The Japanese Society of Developmental Biologists
- The Japanese Society for Clinical Molecular Morphology
- The Japanese Society of Genome Editing
Educational Activity
Course in Charge
- 2023, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
- 2023, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
- 2023, Undergraduate Education, Second Semester, Practice for Fundamental Biology II
- 2023, Undergraduate Education, First Semester, Practice for Fundamental Biology III
- 2023, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
- 2023, Undergraduate Education, 3Term, Laboratory Work in Biology A
- 2023, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
- 2023, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
- 2023, Graduate Education (Master's Program) , First Semester, Exercises in Life Science A
- 2023, Graduate Education (Master's Program) , Second Semester, Exercises in Life Science B
Research Activities
Academic Papers
- ★, Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers, MEDICAL MOLECULAR MORPHOLOGY, 42(2), 63-69, 200906
- ★, Sea urchin arylsulfatase, an extracellular matrix component, is involved in gastrulation during embryogenesis, DEVELOPMENT GENES AND EVOLUTION, 219(6), 281-288, 200906
- Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51(5), 388-396, 201010
- Mechanism for electrosilent Ca2+ transport to cause calcification of spicules in sea urchin embryos., Experimental Cell Research, 159, 80-90, 198507
- Inhibition of 45Ca2+ uptake in the eggs and embryos of the sea urchin, Anthocidaris crassispina, by several calcium antagonists, anion transport inhibitor and chloride transport inhibitors, Journal of Experimental Zoology, 235, 281-288, 19850401
- Inhibitory effects of ethacrynic acid, furosemide, and nifedipine on the calcification of spicules in cultures of micromeres isolated from sea-urchin eggs, Differentiation, 153(30), 197-204, 198603
- ★, Distributions of H+,K+-ATPase and Cl-,HCO3(-)-ATPase in micromere-derived cells of sea urchin embryos, Differentiation, 35(3), 190-196, 1992
- Isolation of cDNA for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development, Developmental Biology, 153(2), 250-258, 19921001
- cDNA cloning of Na+, K+-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 13(6), 833-841, 19960401
- Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene, Developmental Biology, 181, 284-295, 19970401
- Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore, Development Growth and Differentiation, 39, 373-379, 19970401
- A cis-regulatory element within the 5’ flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 39, 469-476, 19970401
- Cloning of cyclin E cDNA of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 14(5), 791-794, 19971001
- ★, Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos, Journal of Experimental Zoology, 280(3), 220-230, 19980215
- Structure and function of a sea urchin orthodenticle-related gene (HpOtx), Developmental Biology, 193(2), 139-145, 19980101
- ★, Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNAs during early development, International Journal of Developmental Biology, 42, 645-651, 199807
- Proximal cis-regulatory elements of sea urchin arylsulfatase gene, Development Growth and Differentiation, 40(5), 537-544, 19981001
- HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation of the sea urchin embryo, Mechanisms of Development, 80, 41-52, 19990401
- Lim1-related homeobox gene (HpLim1) expressed in sea urchin embryo, Development Growth and Differentiation, 41(3), 273-282, 19990601
- Hbox1 and Hbox7 are involved in pattern formation in sea urchin embryos, Development Growth and Differentiation, 41(3), 241-252, 19990601
- Upstream element of the sea urchin arylsulfatase gene serves as an insulator, Cellular and Molecular Biology, 45(5), 555-565, 19990401
- Stage specific effects on sea urchin embryogenesis of Zn2+, Li+, several inhibitors of cAMP-phosphodiestrase and inhibitors of protein synthesis., Development Growth and Differentiation, 25, 249-260, 19830401
- Stage specific effects of Zn2+ on sea urchin embryogenesis, Development Growth and Differentiation, 26, 317-327, 198410
- Carbonic anhydrase activity in developing sea urchin embryos with special reference to calcification of spicules, Cell Differentiation, 18, 257-262, 198606
- Change in the activity of Cl-,HCO3(-)-ATPase in microsome fraction during early development of the sea urchin, Hemicentrotus pulcherrimus, Journal of Biochemistry, 100, 1607-1615, 198612
- Probable role of allylisothiocyanate-sensitive H+,K+-ATPase in spicule calcification in embryos of the sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 29, 57-70, 19870401
- Inhibitory effect of omeprazole, a specific inhibitor of H+,K+-ATPase, on spicule formation in sea urchin embryos and in cultured micromere-derived cells, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29, 591-597, 19870401
- Development of sea urchin embryos in artificial sea water containing Br- in place of Cl-, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29, 599-605, 19870401
- Probable participation of mitochondrial Ca2+ transport in calcification of spicules and morphogenesis in sea urchin embryos, Zoological Science, 5, 93-102, 19880401
- Changes in the activities of H+,K+-ATPase and Na+,K+- ATPase in cultured cells derived from micromeres of sea urchin embryos with special reference to their roles in spicule rod formation, Development Growth and Differentiation, 31, 171-178, 19890401
- Probable contribution of protein phosphorylation by protein kinase C to spicule formation in sea urchin embryos, Development Growth and Differentiation, 32, 335-342, 19900401
- Does protein phosphorylation by protein kinase C support pseudopodial cable growth in cultured micromere-derived cells of the sea urchin, Hemicentrotus pulcherrimus?, Development Growth and Differentiation, 32, 647-655, 19900401
- Change in the activity of Na+,K+-ATPase in embryos of the sea urchin, Hemicentrotus pulcherrimus, during early development, Development Growth and Differentiation, 34, 379-385, 19920401
- Expression of Na+,K+-ATPase alpha-subunit in animalized and vegetalized embryos of the sea urchin Hemicentrotus pulcherrimus, Development Growth and Differentiation, 34, 677-684, 19920401
- Changes in insulin-binding capacity of the plasma membrane fraction during culture in vitro of cells derived from micromeres of 16-cell-stage sea urchin embryos, Development Growth and Differentiation, 36, 289-298, 19940401
- Several cell responses to insulin of cultured cells derived from micromeres, isolated from sea urchin embryos at the 16 cell stage, Development Growth and Differentiation, 36, 397-408, 19940401
- Sox regulates transcription of the sea urchin arylsulfatase gene, DEVELOPMENT GROWTH & DIFFERENTIATION, 42(4), 429-435, 200008
- CAAT sites are required for the activation of the H-pulcherrimus Ars gene by Otx, DEVELOPMENT GENES AND EVOLUTION, 210(12), 583-590, 200012
- HpEts implicated in primary mesenchyme cell differentiation of the sea urchin (Hemicentrotus pulcherrimus) embryo, ZYGOTE, 8, S33-S34, 2000
- Brachyury homolog (HpTa) is involved in the formation of archenteron and secondary mesenchyme cell differentiation in the sea urchin embryo, ZOOLOGY, 104(2), 99-102, 2001
- T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129(22), 5205-5216, 200211
- Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213(1), 44-49, 200302
- The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(1), 61-69, 200402
- A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(4), 335-341, 200408
- The micro1 gene is necessary and sufficient for micromere differentiation and mid/hindgut-inducing activity in the sea urchin embryo, DEVELOPMENT GENES AND EVOLUTION, 215(9), 450-459, 200509
- Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6(5), 572-577, 200606
- Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23(1), 9-21, 200601
- Novel structure of hepatic extracellular matrices containing arylsulfatase A, Okajimas Folia Anatomica Japonica, 90(1), 17-22, 2013
Social Activities
History as Committee Members
- The Committee of the Zoological Society of Japan for equal participation of men and women in science, 2014/09, 2015/09, The Zoological Society of Japan
- The Committee of the Zoological Society of Japan for equal participation of men and women in science, 2012/10, 2014/09, The Zoological Society of Japan
Organizing Academic Conferences, etc.
- 1999/12
- 1991/05