Keiko Mitsunaga-Nakatsubo

Last Updated :2024/04/03

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Assistant Professor
E-mail
kmntnhiroshima-u.ac.jp
Self-introduction
A multicellular organism develops from a single cell-a fertilized egg. During animal development, cells that divide repeatedly change their position and shape, and adhere to form an individual body plan. This process is mediated by multiple extracellular matrix components. To clarify the mechanism and evolution of animal body plan, I am investigating the function of novel extracellular matrix in morphogenesis and its conservation and diversity. I would like to contribute to the elucidation of the pathogenic mechanism of diseases that result from the disruption of the extracellular matrix environment. I am also a member of the committees on gender equality at a university and academic societies.

Basic Information

Academic Degrees

  • Dr. of Science, Waseda University
  • Master of Science, Waseda University

Educational Activity

  • [Bachelor Degree Program] School of Science : Biological Sciences : Biology
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Mathematical and Life Sciences

In Charge of Primary Major Programs

  • Biology

Research Fields

  • Biology;Basic biology;Morphology / Structure
  • Biology;Biological Science;Developmental biology

Research Keywords

  • development
  • cell differentiation
  • cell fate
  • extracellular matrix
  • morphogenesis

Affiliated Academic Societies

  • the Zoological Society of Japan
  • The Japanese Biochemical Society
  • The Japanese Society of Developmental Biologists
  • The Japanese Society for Clinical Molecular Morphology
  • The Japanese Society of Genome Editing

Educational Activity

Course in Charge

  1. 2024, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
  2. 2024, Undergraduate Education, Second Semester, Seminar for Molecular Genetics
  3. 2024, Undergraduate Education, Second Semester, Practice for Fundamental Biology II
  4. 2024, Undergraduate Education, First Semester, Practice for Fundamental Biology III
  5. 2024, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  6. 2024, Undergraduate Education, 3Term, Laboratory Work in Biology A
  7. 2024, Graduate Education (Master's Program) , 2Term, Topical Seminar in Life Science A
  8. 2024, Graduate Education (Master's Program) , 4Term, Topical Seminar in Life Science B
  9. 2024, Graduate Education (Master's Program) , First Semester, Exercises in Life Science A
  10. 2024, Graduate Education (Master's Program) , Second Semester, Exercises in Life Science B

Research Activities

Academic Papers

  1. ★, Cell-surface arylsulfatase A and B on sinusoidal endothelial cells, hepatocytes, and Kupffer cells in mammalian livers, MEDICAL MOLECULAR MORPHOLOGY, 42(2), 63-69, 200906
  2. ★, Sea urchin arylsulfatase, an extracellular matrix component, is involved in gastrulation during embryogenesis, DEVELOPMENT GENES AND EVOLUTION, 219(6), 281-288, 200906
  3. Mammalian arylsulfatase A functions as a novel component of the extracellular matrix, CONNECTIVE TISSUE RESEARCH, 51(5), 388-396, 201010
  4. Mechanism for electrosilent Ca2+ transport to cause calcification of spicules in sea urchin embryos., Experimental Cell Research, 159, 80-90, 198507
  5. Inhibition of 45Ca2+ uptake in the eggs and embryos of the sea urchin, Anthocidaris crassispina, by several calcium antagonists, anion transport inhibitor and chloride transport inhibitors, Journal of Experimental Zoology, 235, 281-288, 19850401
  6. Inhibitory effects of ethacrynic acid, furosemide, and nifedipine on the calcification of spicules in cultures of micromeres isolated from sea-urchin eggs, Differentiation, 153(30), 197-204, 198603
  7. ★, Distributions of H+,K+-ATPase and Cl-,HCO3(-)-ATPase in micromere-derived cells of sea urchin embryos, Differentiation, 35(3), 190-196, 1992
  8. Isolation of cDNA for LCE and HCE, two constituent proteases of the hatching enzyme of Oryzias latipes, and concurrent expression of their mRNAs during development, Developmental Biology, 153(2), 250-258, 19921001
  9. cDNA cloning of Na+, K+-ATPase alpha-subunit from embryos of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 13(6), 833-841, 19960401
  10. Two isoforms of orthodenticle-related proteins (HpOtx) bind to the enhancer element of sea urchin arylsulfatase gene, Developmental Biology, 181, 284-295, 19970401
  11. Oral-aboral ectoderm differentiation of the sea urchin embryos is disrupted in response to calcium ionophore, Development Growth and Differentiation, 39, 373-379, 19970401
  12. A cis-regulatory element within the 5’ flanking region of arylsulfatase gene of sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 39, 469-476, 19970401
  13. Cloning of cyclin E cDNA of the sea urchin, Hemicentrotus pulcherrimus, Zoological Science, 14(5), 791-794, 19971001
  14. ★, Arylsulfatase exists as non-enzymatic cell surface protein in sea urchin embryos, Journal of Experimental Zoology, 280(3), 220-230, 19980215
  15. Structure and function of a sea urchin orthodenticle-related gene (HpOtx), Developmental Biology, 193(2), 139-145, 19980101
  16. ★, Differential expression of sea urchin Otx isoform (HpOtxE and HpOtxL) mRNAs during early development, International Journal of Developmental Biology, 42, 645-651, 199807
  17. Proximal cis-regulatory elements of sea urchin arylsulfatase gene, Development Growth and Differentiation, 40(5), 537-544, 19981001
  18. HpEts, an ets-related transcription factor implicated in primary mesenchyme cell differentiation of the sea urchin embryo, Mechanisms of Development, 80, 41-52, 19990401
  19. Lim1-related homeobox gene (HpLim1) expressed in sea urchin embryo, Development Growth and Differentiation, 41(3), 273-282, 19990601
  20. Hbox1 and Hbox7 are involved in pattern formation in sea urchin embryos, Development Growth and Differentiation, 41(3), 241-252, 19990601
  21. Upstream element of the sea urchin arylsulfatase gene serves as an insulator, Cellular and Molecular Biology, 45(5), 555-565, 19990401
  22. Stage specific effects on sea urchin embryogenesis of Zn2+, Li+, several inhibitors of cAMP-phosphodiestrase and inhibitors of protein synthesis., Development Growth and Differentiation, 25, 249-260, 19830401
  23. Stage specific effects of Zn2+ on sea urchin embryogenesis, Development Growth and Differentiation, 26, 317-327, 198410
  24. Carbonic anhydrase activity in developing sea urchin embryos with special reference to calcification of spicules, Cell Differentiation, 18, 257-262, 198606
  25. Change in the activity of Cl-,HCO3(-)-ATPase in microsome fraction during early development of the sea urchin, Hemicentrotus pulcherrimus, Journal of Biochemistry, 100, 1607-1615, 198612
  26. Probable role of allylisothiocyanate-sensitive H+,K+-ATPase in spicule calcification in embryos of the sea urchin, Hemicentrotus pulcherrimus, Development Growth and Differentiation, 29, 57-70, 19870401
  27. Inhibitory effect of omeprazole, a specific inhibitor of H+,K+-ATPase, on spicule formation in sea urchin embryos and in cultured micromere-derived cells, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29, 591-597, 19870401
  28. Development of sea urchin embryos in artificial sea water containing Br- in place of Cl-, DEVELOPMENT GROWTH AND DIFFERENTIATION, 29, 599-605, 19870401
  29. Probable participation of mitochondrial Ca2+ transport in calcification of spicules and morphogenesis in sea urchin embryos, Zoological Science, 5, 93-102, 19880401
  30. Changes in the activities of H+,K+-ATPase and Na+,K+- ATPase in cultured cells derived from micromeres of sea urchin embryos with special reference to their roles in spicule rod formation, Development Growth and Differentiation, 31, 171-178, 19890401
  31. Probable contribution of protein phosphorylation by protein kinase C to spicule formation in sea urchin embryos, Development Growth and Differentiation, 32, 335-342, 19900401
  32. Does protein phosphorylation by protein kinase C support pseudopodial cable growth in cultured micromere-derived cells of the sea urchin, Hemicentrotus pulcherrimus?, Development Growth and Differentiation, 32, 647-655, 19900401
  33. Change in the activity of Na+,K+-ATPase in embryos of the sea urchin, Hemicentrotus pulcherrimus, during early development, Development Growth and Differentiation, 34, 379-385, 19920401
  34. Expression of Na+,K+-ATPase alpha-subunit in animalized and vegetalized embryos of the sea urchin Hemicentrotus pulcherrimus, Development Growth and Differentiation, 34, 677-684, 19920401
  35. Changes in insulin-binding capacity of the plasma membrane fraction during culture in vitro of cells derived from micromeres of 16-cell-stage sea urchin embryos, Development Growth and Differentiation, 36, 289-298, 19940401
  36. Several cell responses to insulin of cultured cells derived from micromeres, isolated from sea urchin embryos at the 16 cell stage, Development Growth and Differentiation, 36, 397-408, 19940401
  37. Sox regulates transcription of the sea urchin arylsulfatase gene, DEVELOPMENT GROWTH & DIFFERENTIATION, 42(4), 429-435, 200008
  38. CAAT sites are required for the activation of the H-pulcherrimus Ars gene by Otx, DEVELOPMENT GENES AND EVOLUTION, 210(12), 583-590, 200012
  39. HpEts implicated in primary mesenchyme cell differentiation of the sea urchin (Hemicentrotus pulcherrimus) embryo, ZYGOTE, 8, S33-S34, 2000
  40. Brachyury homolog (HpTa) is involved in the formation of archenteron and secondary mesenchyme cell differentiation in the sea urchin embryo, ZOOLOGY, 104(2), 99-102, 2001
  41. T-brain homologue (HpTb) is involved in the archenteron induction signals of micromere descendant cells in the sea urchin embryo, DEVELOPMENT, 129(22), 5205-5216, 200211
  42. Utilization of a particle gun DNA introduction system for the analysis of cis-regulatory elements controlling the spatial expression pattern of the arylsulfatase gene (HpArs) in sea urchin embryos, DEVELOPMENT GENES AND EVOLUTION, 213(1), 44-49, 200302
  43. The Otx binding site is required for the activation of HpOtxL mRNA expression in the sea urchin, Hemicentrotus pulcherrimus, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(1), 61-69, 200402
  44. A new G-stretch-DNA-binding protein, Unichrom, displays cell-cycle-dependent expression in sea urchin embryos, DEVELOPMENT GROWTH & DIFFERENTIATION, 46(4), 335-341, 200408
  45. The micro1 gene is necessary and sufficient for micromere differentiation and mid/hindgut-inducing activity in the sea urchin embryo, DEVELOPMENT GENES AND EVOLUTION, 215(9), 450-459, 200509
  46. Developmental expression of HpNanos, the Hemicentrotus pulcherrimus homologue of nanos, GENE EXPRESSION PATTERNS, 6(5), 572-577, 200606
  47. Unichrom, a novel nuclear matrix protein, binds to the Ars insulator and canonical MARs, ZOOLOGICAL SCIENCE, 23(1), 9-21, 200601
  48. Novel structure of hepatic extracellular matrices containing arylsulfatase A, Okajimas Folia Anatomica Japonica, 90(1), 17-22, 2013

Social Activities

History as Committee Members

  1. The Committee of the Zoological Society of Japan for equal participation of men and women in science, 2014/09, 2015/09, The Zoological Society of Japan
  2. The Committee of the Zoological Society of Japan for equal participation of men and women in science, 2012/10, 2014/09, The Zoological Society of Japan

Organizing Academic Conferences, etc.

  1. 1999/12
  2. 1991/05