AKIFUMI HIGASHIURA

Last Updated :2024/06/04

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Assistant Professor
E-mail
ahigashiurahiroshima-u.ac.jp

Basic Information

Major Professional Backgrounds

  • 2010/04/01, 2012/03/31, Osaka University, Institute for Protein Research, Postdoctoral Fellow
  • 2012/04/01, 2016/03/31, Osaka University, Institute for protein research, Assistant Professor (Special Appointment)
  • 2016/04/01, 2018/03/31, Osaka University, Institute for protein research, Assistant Professor
  • 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor
  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor

Educational Backgrounds

  • Osaka University, Japan, 2000/04, 2004/03
  • Osaka University, Japan, 2004/04, 2006/03
  • Osaka University, Japan, 2006/04, 2010/03

Academic Degrees

  • MSc, Osaka University
  • Ph.D, Osaka University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;Virology
  • Biology;Biological Science;Structural biochemistry

Research Keywords

  • X-ray crystallography, Structural biology, virology

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, Year, Biological Responses
  2. 2024, Graduate Education (Master's Program) , 3Term, Seminar on Host Defense

Research Activities

Academic Papers

  1. Magnetic interaction in superparamagnetic Co-Pt nanoparticles synthesized in protein crystal, IEEE Transactions on Magnetics, 20230630
  2. Virus purification highlights the high susceptibility of SARS-CoV-2 to a chlorine-based disinfectant, chlorous acid, PLOS ONE, 18(7), 20230714
  3. Capture and neutralization of SARS-CoV-2 and influenza virus by algae-derived lectins with high-mannose and core fucose specificities, MICROBIOLOGY AND IMMUNOLOGY, 67(7), 334-344, 202307
  4. Solid-state Thermal Transformation of Methylammonium Monomolybdate to Polyoxomolybdates and their Applications as Staining Reagents for Transmission Electron Microscopy Observations of Viruses, CHEMISTRY LETTERS, 52(3), 167-171, 202303
  5. ★, Structural basis of spike RBM-specific human antibodies counteracting broad SARS-CoV-2 variants, COMMUNICATIONS BIOLOGY, 6(1), 20230411
  6. Vaccination with the Omicron spike RBD boosts broadly neutralizing antibody levels and confers sustained protection even after acquiring immunity to the original antigen, INTERNATIONAL IMMUNOLOGY, 35(4), 197-207, 20230404
  7. Thermal Structure Transformation of Methylammonium Vanadate and it's Application as a Negative Staining Reagent for Observing SARS-CoV-2, EUROPEAN JOURNAL OF INORGANIC CHEMISTRY, 2022(26), 20220919
  8. Synthesis of superparamagnetic Co-Pt nanoparticle in Pyrococcus furiosus virus-like particle crystal, JOURNAL OF PHYSICS AND CHEMISTRY OF SOLIDS, 169, 202210
  9. Ethanol Susceptibility of SARS-CoV-2 and Other Enveloped Viruses, BIOCONTROL SCIENCE, 26(3), 177-180, 2021
  10. Effects of Traditional Kampo Drugs and Their Constituent Crude Drugs on Influenza Virus Replication In Vitro: Suppression of Viral Protein Synthesis by Glycyrrhizae Radix, EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE, 2019, 20191203
  11. Electrospray sample injection for single-particle imaging with x-ray lasers, SCIENCE ADVANCES, 5(5), 201905
  12. Molecular structure of a 5,10-methylenetetrahydrofolate dehydrogenase from the silkworm Bombyx mori, FEBS Open Bio., 2211-5463.12595, 2019
  13. An assembly intermediate structure of Rice dwarf virus reveals a hierarchical outer capsid shell assembly mechanism, Structure, 5(27(3)), 439-448, 2019
  14. Characterization of a diazinon-metabolizing glutathione S-transferase in the silkworm Bombyx more by X-ray crystallography and genie editing analysis, Sci. Rep., 8, 16835, 2018
  15. Hierarchical structure assembly model of rice dwarf virus particle formation., Biophys. Rev., 10(2), 659-665, 2018
  16. Molecular structure of a prostaglandin D synthase requiring glutathione from the brown planthopper, Nilaparvata lugens., Biochem. Biophys. Res. Commun., 14(492), 166-171, 2017
  17. Technical Improvements for High-resolution X-ray Crystallography from Space Grown Crystals, IJMSA, 34(1), 340105, 2017
  18. Coherent diffraction of single Rice Dwarf Virus particles using hard X-rays at the Linac Coherent Light Source, Sci Data., 1(3), 16064, 2016
  19. Structural characterization of an aldo-keto reductase (AKR2E5) from the silkworm Bombyx mori, Biochem Biophys Res Commun., 20(474(1)), 104-110, 2016
  20. SPring-8 BL44XU, Beamline Designed for Structure Analysis of Large Biological Macromolecular Assemblies., AIP Conf. Proc., 1741(030028), 2016
  21. Electron microscopic imaging revealed the flexible filamentous structure of the cell attachment protein P2 of Rice dwarf virus located around the icosahedral fivefold axes., J. Biochem., 159(2), 181-190, 2016
  22. Structural characterization of the catalytic site of a Nilaparvata lugens delta-class glutathione transferase., Arch. Biochem. Biophys., 566, 36-42, 2015
  23. X-ray crystallographic structure of RNase Po1 that exhibits anti-tumor activity., Biol. Pharm. Bull., 37(6), 968-978, 2014
  24. High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method., J. Synchrotron Radiat., 20(6), 989-993, 2013
  25. Crystal structure of a Bombyx mori Sigma-class glutathione transferase exhibiting prostaglandin E synthase activity., Biochim Biophys Acta., 1830(6), 3711-3718, 2013
  26. Crystallographic analysis reveals octamerization of viroplasm matrix protein P9-1 of rice black streaked dwarf virus., J. Virol., 86(2), 746-756, 2012
  27. Improvement of Protein Crystal Quality using the Microgravity Environment: From Small Proteins to Viruses., J. Jpn. Soc. Microgravity Appl., 29(3), 132-137, 2012
  28. Viroplasm matrix protein Pns9 from rice gall dwarf virus forms an octameric cylindrical structure., J. Gen. Virol., 92(9), 2214-2221, 2011
  29. High-resolution X-ray crystal structure of bovine H-protein at 0.88 Å resolution., Acta Crystallogr. D Biol. Crystallogr., 147(6), 843-850, 2010
  30. The functional organization of the internal components of Rice dwarf virus., J. Biochem., 147(6), 843-850, 2010
  31. Magnetic properties of PfV encapsulating Ni ions., J. Magn. Soc. Jpn., 33, 473-476, 2009
  32. Structural evolution of reoviridae revealed by oryzavirus in acquiring the second capsid shell., J. Virol., 82, 11344-11353, 2008
  33. Crystallization and preliminary X-ray diffraction analysis of recombinant hepatitis E virus-like particle., Acta Crystallogr Sect F Struct Biol Cryst Commun., 64, 318-322, 2008

Invited Lecture, Oral Presentation, Poster Presentation

  1. Sequential exposure to different SARS-CoV-2 antigens induces broadly neutralizing antibodies in mice, Hitoshi Azuma, Yohei Kawano, Akifumi Higashiura, Yasuo Kitajima, Shun Ohki, Tomoharu Yasuda, The 52nd Annual Meeting of The Japanese Society for Immunology, 2024/01/17, Without Invitation, English
  2. Structural investigations of cargo molecules inside icosahedrally symmetric encapsulin by VUVCD spectroscopic measurements, Shiori Kumamoto, Akima Yamamoto, Yu Shiratsuchi, Koichi Matsuo, Akifumi Higashiura, Daisuke Hira, The 19th International Conference on Chiroptical Spectroscopy, 2023/09/17, Without Invitation, English
  3. Flash X-ray Diffraction Imaging of Paramecium bursaria Chlorella Virus-1 from Characteristic Patterns of Icosahedrons, Akifumi Higashiura, Gijs van der Schot, Max Hantke, Jing Liu, Takashi Yamada, Benedikt Daurer, Flipe R.N.C. Maia, Tomas Ekeberg, Janos Hajdu and Atsushi Nakagawa, International Symposium on Diffraction Structural Biology 2019, 2019/10, Without Invitation, English
  4. Structural studies of viroplasm major component protein Pns12 from Rice Dwarf Virus, Higashiura A., Miyazaki N., Nakamichi Y., Tsutsumi K., Kajiura N., Nakagawa A., AsCA 2018/CRYSTAL 32, 2018/12, Without Invitation, English, Auckland, New Zealand
  5. Structural studies of Rice Dwarf Virus, Akifumi Higashiura, Taiwan-Japan Biomedicine Conference National Tsing Hua University-Osaka University bilateral symposium, 2017/10, With Invitation, English, Osaka
  6. Structure assembly mechanism of Rice Dwarf Virus, Higashiura A., Nakamichi Y., Miyazaki N., Tsutsumi K., Iwasaki K., Murata K., Omura T., Nakagawa A., 24th Congress and General Assembly of the International Union of Crystallography, 2017/08, Without Invitation, English, Hyderabad, India
  7. Approaches to the high resolution X-ray crystallography using by the microgravity environment, Akifumi Higashiura, The 2nd International Symposium (SSPC-2) Space Science of High Quality Protein Crystallization Technology, 2016/10, With Invitation, English, Tokyo
  8. SPring-8 BL44XU, A Beamline Designed for Structure Analysis of Large Biological Macromolecular Assemblies, Yamashita E., Higashiura A., Yoshimura M., Hasegawa K., Furukawa Y., Kumasaka T., Ueno G., Yamamoto M., Tsukihara T., Nakagawa A., International Symposium on Diffraction Structural Biology, 2016/08, Without Invitation, English, Knoxville, US
  9. Approaches for Coherent X-ray Diffraction Imaging of Paramecium bursaria Chlorella virus-1, Higashiura A., van der Schot G., Hantke M., Liu J., Yamada T., Hajdu J., Nakagawa A., The 14th Conforence of the Asian Crystallographic Association, 2015/12, Without Invitation, English, Kolkata, India
  10. The high-resolution X-ray crystal structure of the capping enzyme from Rice Dwarf Virus, Nakamichi Y., Higashiura A., Narita H., Ichiki T., Hagiwara K., Omura T., Nakagawa A., The 14th Conforence of the Asian Crystallographic Association, 2015/12, Without Invitation, English, Kolkata, India
  11. SPring-8 BL44XU, Beamline Designed for Structure Analysis of Large Biological Macromolecular Assemblies, Higashiura A., Yamashita, E., Yoshimura M., Hasegawa K., Furukawa Y., Kumasaka T., Ueno G., Yamamoto M., Tsukihara T., Nakagawa A., 12th International Conference on Synchrotron Radiation Instrumentation, 2015/07, Without Invitation, English, New York, US
  12. Detailed cell entry mechanism of Rice dwarf virus (RDV), 2014/09, Without Invitation, Japanese
  13. IPR Beamline for Macromolecular Assemblies at SPring-8, Higashiura A., Yoshimura M., Hasegawa K., Furukawa Y., Ohata T., Kumasaka T., Ueno G., Yamamoto M., Yoshikawa S., Tsukihara T., Nakagawa A., 23rd Congress and General Assembly of the International Union of Crystallography, 2014/08, Without Invitation, English, Montreal, Canada
  14. Approaches to coherent X-ray diffraction imaging of single virus particle using X-ray free-electron laser, Higashiura A., Murakami M., Iwasaki K., Yamashita E., Takeda K., Hanazono Y., Takaba K., Hibi M., Tomisaki Y., Miki K., Nakagwa A., 2013/10, Without Invitation, English
  15. High-resolution X-ray crystal structure of bovine H-protein using a high-pressure cryocooling method, Higashiura A., Ohta K., Masaki M., Inaka K., Tanaka, H, Nakagawa A., 4th International symposium on diffraction structural biology, 2013/06, Without Invitation, English, Nagoya
  16. Synchrotron Radiation Beamline for Macromolecular Assemblies at SPring-8, operated by the Institute for Protein Research (BL44XU), Yamashita E., Higashiura A., Suzuki M., Yoshimura M., Hasegawa K., Furukawa Y., Kumasaka T., Ueno G., Yamamoto M., Yoshikawa S., Tsukihara T., Nakagawa A., 4th International symposium on diffraction structural biology, 2013/06, Without Invitation, English, Nagoya