ATSUHIKO ISHIDA

Last Updated :2021/04/16

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
aishidahiroshima-u.ac.jp
Other Contact Details
1-7-1,Kagamiyama,Higashi-Hiroshima, Japan
TEL : (+81) FAX : (+81)
Self-introduction
I am interested in intracellular signal transduction, especially protein phosphorylation and dephosphorylation. I am now studying about multifunctional CaM kinases, which play important roles in Ca2+ signaling, and CaM kinase phosphatase (CaMKP/PPM1F/POPX2), which is involved in their regulation. CaMKP has been reported to be involved in pathogenesis of various diseases such as cancer and depression.

Basic Information

Major Professional Backgrounds

  • 1989/04/01, 1990/02/28, Okayama University
  • 1990/03/01, 2007/03/31, Asahikawa Medical College, Faculty of Medicine, Research Associate
  • 2007/04/01, 2014/03/31, Hiroshima University, Graduate School of Integrated Arts and Sciences, Associate Professor
  • 2014/04/01, 2019/03/31, Hiroshima University, Graduate School of Integrated Arts and Sciences, Professor

Academic Degrees

  • Doctor of Engineering, Kyoto University
  • Master of Engineering, Kyoto University

Educational Activity

  • 【Bachelor Degree Program】School of Integrated Arts and Sciences : Department of Integrated Arts and Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Life and Environmental Sciences
  • 【Master's Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Life and Environmental Sciences
  • 【Doctoral Program】Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science

In Charge of Primary Major Programs

  • Integrated Arts and Sciences

Research Fields

  • Complex systems;Biomolecular science;Chemical biology
  • Complex systems;Biomolecular science;Biomolecular chemistry
  • Biology;Biological Science;Structural biochemistry
  • Biology;Biological Science;Functional biochemistry

Research Keywords

  • enzyme
  • protein kinase
  • protein phosphatase
  • phosphorylation/dephosphorylation
  • synaptic vesicle
  • bioanalytical chemistry

Affiliated Academic Societies

  • Japanese Biochemical Society
  • Society for neuroscience
  • Japanese Association for Protein Phosphatase Research

Educational Activity

Course in Charge

  1. 2021, Liberal Arts Education Program1, 4Term, Enzymes supporting our life
  2. 2021, Liberal Arts Education Program1, 3Term, Experimental Methods and Laboratory Work in Chemistry I
  3. 2021, Liberal Arts Education Program1, 4Term, Experimental Methods and Laboratory Work in Chemistry II
  4. 2021, Undergraduate Education, 2Term, Introduction to Life Sciences
  5. 2021, Undergraduate Education, 2Term, Functional Biochemistry I
  6. 2021, Undergraduate Education, 3Term, Functional Biochemistry II
  7. 2021, Undergraduate Education, 1Term, Laboratory Work in Life Sciences C
  8. 2021, Undergraduate Education, 1Term, Practical Methods in Life Sciences C
  9. 2021, Undergraduate Education, 4Term, Functional Biochemistry III
  10. 2021, Undergraduate Education, 1Term, Fundamentals of Life Science
  11. 2021, Undergraduate Education, 1Term, Fundamentals of Biochemistry I
  12. 2021, Undergraduate Education, 2Term, Fundamentals of Biochemistry II
  13. 2021, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Life and Environmental Sciences
  14. 2021, Graduate Education (Master's Program) , 1Term, Exercises in Life and Environmental Sciences A
  15. 2021, Graduate Education (Master's Program) , 2Term, Exercises in Life and Environmental Sciences A
  16. 2021, Graduate Education (Master's Program) , 3Term, Exercises in Life and Environmental Sciences B
  17. 2021, Graduate Education (Master's Program) , 4Term, Exercises in Life and Environmental Sciences B
  18. 2021, Graduate Education (Master's Program) , 1Term, Introduction to Integrated Arts and Sciences
  19. 2021, Graduate Education (Master's Program) , Second Semester, Functional biochemistry
  20. 2021, Graduate Education (Doctoral Program) , 2Term, Seminar in Integrated Arts and Scirnces II
  21. 2021, Graduate Education (Doctoral Program) , 4Term, Exercises in Integrated Life Sciences
  22. 2021, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins, Biochim. Biophys. Acta - Molecular Cell Research, 1868, 118931, 2021
  2. Contribution of DHA diols (19,20-DHDP) produced by cytochrome P450s and soluble epoxide hydrolase to the beneficial effects of DHA supplementation in the brains of rotenone-induced rat models of Parkinson's disease, Biochim. Biophys. Acta - Molecular and Cell Biology of Lipids, 1866(2), 158858, 20210201
  3. ★, Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 530(3), 513-519, 20200924
  4. ★, Biochemical characterization of four splice variants of mouse Ca2+/calmodulin-dependent protein kinase Idelta, Journal of Biochemistry, in press, https://doi.org/10.1093/jb/mvaa117, 20201102
  5. ★, Autoactivation of C-terminally truncated Ca2+/calmodulin-dependent protein kinase (CaMK) Idelta via CaMK kinase-independent autophosphorylation., Arch Biochem Biophys, 668, 29-38, 20190507
  6. ★, Functions and dysfunctions of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E., Archives of biochemistry and biophysics, 640, 83-92, 20180215
  7. Progression of vasogenic edema induced by activated microglia under permanent middle cerebral artery occlusion., Biochemical and biophysical research communications, 496(2), 582-587, 20180117
  8. ★, Facile preparation of highly active casein kinase 1 using Escherichia coli constitutively expressing lambda phosphatase., Analytical biochemistry, 549, 99-106, 20180317
  9. Characterization of CoPK02, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea., Bioscience, biotechnology, and biochemistry, 82(8), 1335-1343, 20180420
  10. ★, High-performance CaMKI: A highly active and stable form of CaMKI delta produced by high-level soluble expression in Escherichia coli, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 475(3), 277-282, 20160701
  11. ★, Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) interacts with neurofilament L and inhibits its filament association, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 477(4), 820-825, 20160902
  12. Effects of sex steroid hormones and their metabolites on neuronal injury caused by oxygen-glucose deprivation/reoxygenation in organotypic hippocampal slice cultures, STEROIDS, 113, 71-77, 20160902
  13. ★, The phosphatase-resistant isoform of CaMKI, Ca2+/calmodulin-dependent protein kinase Id (CaMKId), remains in its 'Primed' form without Ca2+stimulation., Biochemistry, 54, 3617-3630, 201504
  14. ★, Regulation of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) by protocadherin-gamma C5 (Pcdh-gamma C5), ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 585, 109-120, 201504
  15. Dual Role of Superoxide Dismutase 2 Induced in Activated Microglia OXIDATIVE STRESS TOLERANCE AND CONVERGENCE OF INFLAMMATORY RESPONSES, JOURNAL OF BIOLOGICAL CHEMISTRY, 290(37), 22805-22817, 201504
  16. Neuroprotection elicited by nerve growth factor and brain-derived neurotrophic factor released from astrocytes in response to methylmercury, ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 40(1), 199-205, 201504
  17. Protective Actions of 17 beta-Estradiol and Progesterone on Oxidative Neuronal Injury Induced by Organometallic Compounds, OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 201504
  18. Long noncoding RNA, CCDC26, controls myeloid leukemia cell growth through regulation of KIT expression, MOLECULAR CANCER, 14, 201504
  19. Selective estrogen-receptor modulators suppress microglial activation and neuronal cell death via an estrogen receptor-dependent pathway, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 145, 85-93, 201504
  20. Suppressive effects of 17 beta-estradiol on tributyltin-induced neuronal injury via Akt activation and subsequent attenuation of oxidative stress, LIFE SCIENCES, 99(1-2), 24-30, 20140318
  21. ★, An Active C-Terminally Truncated Form of Ca2+/Calmodulin-Dependent Protein Kinase Phosphatase-N (CaMKP-N/PPM1E), BIOMED RESEARCH INTERNATIONAL, 2013
  22. ★, Ink-native electrophoresis: An alternative to blue-native electrophoresis more suitable for in-gel detection of enzymatic activity, ANALYTICAL BIOCHEMISTRY, 440(2), 142-144, 20130915
  23. Allopregnanolone-mediated protective effects of progesterone on tributyltin-induced neuronal injury in rat hippocampal slices, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 135, 1-6, 20130501
  24. De Novo Synthesized Estradiol Protects against Methylmercury-Induced Neurotoxicity in Cultured Rat Hippocampal Slices, PLOS ONE, 8(2), 20130206
  25. ★, Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from a-ketoglutarate for vesicular loading, JOURNAL OF NEUROCHEMISTRY, 121(2), 184-196, 201204
  26. ★, A sensitive method based on fluorescence-detected circular dichroism for protein local structure analysis, ANALYTICAL BIOCHEMISTRY, 430(2), 179-184, 20121115
  27. Tributyltin induces oxidative stress and neuronal injury by inhibiting glutathione S-transferase in rat organotypic hippocampal slice cultures, NEUROCHEMISTRY INTERNATIONAL, 60(8), 782-790, 201206
  28. Functional processing of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N): Evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 517(1), 43-52, 20120101
  29. Phosphorylation and activation of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) by Ca2+/calmodulin-dependent protein kinase I (CaMKI), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 422(4), 703-709, 20120615
  30. Regulation of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP) by oxidation/reduction at Cys-359, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 526(1), 9-15, 20121001
  31. CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea., Biochim Biophys Acta., 1810, 620-629, 20110401
  32. Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus., Gen Comp Endocrinol., 171(1), 28-32, 20110301
  33. ★, In-Gel Protein Phosphatase Assays and Other Useful Methods for the Detection of Protein Phosphatase Activities, ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 11(1), 47-53, 201101
  34. A minimum size homologue of Ca2+/calmodulin-dependent protein kinase IV naturally occurring in zebrafish, JOURNAL OF BIOCHEMISTRY, 147(6), 857-865, 201006
  35. Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model, FEBS LETTERS, 584(18), 3949-3954, 20100924
  36. ★, Synaptic Vesicle-bound Pyruvate Kinase can Support Vesicular Glutamate Uptake, Neurochem Res, 34(5), 807-818, 20090501
  37. Social isolation stimulates hippocampal estradiol syntheses, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 379(2), 480-484, 20090206
  38. Retinoic Acid Stimulates 17 beta-Estradiol and Testosterone Synthesis in Rat Hippocampal Slice Cultures, ENDOCRINOLOGY, 150(9), 4260-4269, 200909
  39. Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP) is indispensable for normal embryogenesis in zebrafish, Danio rerio, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 488(1), 48-59, 20090801
  40. ★, Negative regulation of multifunctional Ca(2+)/calmodulin-dependent protein kinases: physiological and pharmacological significance of protein phosphatases, BRITISH JOURNAL OF PHARMACOLOGY, 154(4), 729-740, 200806
  41. Tyrosine kinase activity of a Ca(2+)/calmodulin-dependent protein kinase II catalytic fragment, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 377(2), 648-652, 20081212
  42. ★, Recent advances in technologies to analyze protein kinases., J Pharmacol Sci, 103, 5-11, 20070101
  43. ★, Inhibitors of the Ca2+/calmodulin-dependent protein kinase phosphatase family (CaMKP and CaMKP-N), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 363(3), 715-721, 20071123
  44. Knockdown of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase causes developmental abnormalities in zebrafish., Arch Biochem Biophys, 457, 205-216, 20070401
  45. Synthesis of caged peptides using caged lysine: Application to the synthesis of caged AIP, a highly specific inhibitor of calmodulin-dependent protein kinase II, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 9(8), 1093-1096, 1999
  46. Phosphorylation and activation of Ca2+/calmodulin-dependent protein kinase phosphatase by Ca2+/calmodulin-dependent protein kinase II, FEBS LETTERS, 456(2), 249-252, 1999
  47. A novel protein phosphatase that dephosphorylates and regulates Ca2+/calmodulin-dependent protein kinase II, JOURNAL OF BIOLOGICAL CHEMISTRY, 273(4), 1904-1910, 1998
  48. Critical amino acid residues of AIP, a highly specific inhibitory peptide of calmodulin-dependent protein kinase II, FEBS LETTERS, 427(1), 115-118, 1998
  49. Analysis of protein-protein interaction by two-dimensional affinity electrophoresis, ANALYTICAL BIOCHEMISTRY, 262(1), 90-92, 1998
  50. Assay of protein phosphatase activities with phosphopeptide-magnetic bead conjugates, SEIKAGAKU, 70(11), 1360-1363, 1998
  51. Regulation of multifunctional Ca2+/calmodulin-dependent protein kinases by Ca2+/calmodulin-dependent protein kinase phosphatase, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 253(1), 159-163, 1998
  52. Assay of protein phosphatase activities with phosphopeptide-magnetic particle conjugates, ANALYTICAL BIOCHEMISTRY, 254(1), 152-155, 1997
  53. A new peptide conjugate as a highly specific substrate for MAP kinase, JOURNAL OF BIOCHEMISTRY, 122(1), 168-172, 1997
  54. Detection of protein phosphatase activities in sodium dodecyl sulfate-polyacrylamide gel using peptide substrates, ANALYTICAL BIOCHEMISTRY, 245(2), 149-153, 1997
  55. Calcium- and calmodulin-dependent phosphorylation of AMPA type glutamate receptor subunits by endogenous protein kinases in the post-synaptic density, MOLECULAR BRAIN RESEARCH, 46(1-2), 338-342, 1997
  56. Evidence that autophosphorylation at Thr-286/Thr-287 is required for full activation of calmodulin-dependent protein kinase II, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1311(3), 211-217, 1996
  57. NOVEL HIGHLY SPECIFIC AND POTENT INHIBITOR OF CALMODULIN-DEPENDENT PROTEIN-KINASE-II, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 212(3), 806-812, 1995
  58. STABILIZATION OF CALMODULIN-DEPENDENT PROTEIN-KINASE-II THROUGH THE AUTOINHIBITORY DOMAIN, JOURNAL OF BIOLOGICAL CHEMISTRY, 270(5), 2163-2170, 1995
  59. Requirement of calmodulin-dependent protein kinase II in cyclic ADP-ribose-mediated intracellular Ca2+ mobilization, JOURNAL OF BIOLOGICAL CHEMISTRY, 270(51), 30257-30259, 1995
  60. PHOSPHORYLATION OF RABPHILIN-3A BY CALMODULIN-DEPENDENT PROTEIN-KINASE-II, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 205(3), 1776-1784, 1994
  61. MICROBIOLOGICAL ACTIVITIES OF NUCLEOTIDE LOOP-MODIFIED ANALOGS OF VITAMIN-B-12, ARCHIVES OF MICROBIOLOGY, 161(4), 293-299, 1994
  62. ADENOSYLCOBINAMIDE METHYL PHOSPHATE AS A PSEUDOCOENZYME FOR DIOL DEHYDRASE, BIOCHEMISTRY, 32(6), 1535-1540, 1993
  63. ROLES OF THE D-RIBOSE AND 5,6-DIMETHYLBENZIMIDAZOLE MOIETIES OF THE NUCLEOTIDE LOOP OF ADENOSYLCOBALAMIN IN MANIFESTATION OF COENZYMIC FUNCTION IN THE DIOL DEHYDRASE REACTION, JOURNAL OF BIOLOGICAL CHEMISTRY, 266(9), 5430-5437, 1991
  64. ACCELERATION OF CLEAVAGE OF THE CARBON COBALT BOND OF STERICALLY HINDERED ALKYLCOBALAMINS BY BINDING TO APOPROTEIN OF DIOL DEHYDRASE, BIOCHEMISTRY, 27(20), 7677-7681, 1988
  65. ★, In-gel kinase assay revisited, SEIBUTSU BUTSURI KAGAKU, 58(2), 62-64, 2014
  66. Cellular localization of CoPK12, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea, is regulated by N-myristoylation, JOURNAL OF BIOCHEMISTRY, 156(1), 51-61, 2014
  67. Evaluation of neurotoxicity of trimethyltin in rat brain under various housing conditions, NEUROSCIENCE RESEARCH, 71, E340-E340, 2011
  68. Effect of housing conditions on neurotoxicity of trimethyltin in rat brain, NEUROSCIENCE RESEARCH, 68, E258-E258, 2010
  69. ENVIRONMENTAL ENRICHMENT INCREASES MRNA CONTENTS OF ALLOPREGNANOLONE SYNTHETIC ENZYMES, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 293-293, 2009
  70. Neuroprotective effect of estradiol on methylmercury-induced neuronal cell toxicity in rat hippocampal slices, NEUROSCIENCE RESEARCH, 65, S220-S220, 2009
  71. Social isolation increases mRNA contents of estradiol synthetic enzymes, NEUROSCIENCE RESEARCH, 61, S187-S187, 2008
  72. Identification and Characterization of CaMKP-N, Nuclear Calmodulin-Dependent Protein Kinase Phosphatase, Journal of Biochemistry, 130(6), 833-840, 20011201
  73. EPR Spectroscopic Evidence for the Mechanism Based Inactivation of Adenosylcobalamin-Dependent Diol Dehydratase by Coenzyme Analogs., J Biochem (Tokyo), 124(3), 598-601, 1998
  74. Detection of a Variety of Ser/Thr Protein Kinases Using a Synthetic Peptide with Multiple Phosphorylation Sites., J Biochem (Tokyo), 126(6), 991-995, 1999
  75. Substrate Specificity of Ca2+/Calmodulin-Dependent Protein Kinase Phosphatase: Kinetic Studies Using Synthetic Phosphopeptides as Model Substrates., J Biochem (Tokyo), 129(5), 745-753, 2001
  76. ★, Identification of major Ca^<2+>/calmodulin-dependent protein kinase phosphatase-binding proteins in brain. Biochemical analysis of the interaction., ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 435(1), 134-146, 200503
  77. Generation of a polyclonal antibody that simultaneously detects multiple Ser/Thr protein kinases, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 60(1), 13-22, 200407
  78. ★, Stimulation of Ca^<2+>/calmodulin-dependent protein kinase phosphatase by polycations, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 408(2), 229-238, 200212
  79. ★, Phosphorylation of calmodulin by Ca2+/calmodulin-dependent protein kinase IV, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 407(1), 72-82, 200211
  80. ★, Protein phosphatases that regulate multifunctional Ca2+/calmodulin-dependent protein kinases: from biochemistry to pharmacology, Pharmacology and Therapeutics, 100(3), 291-305, 200312
  81. Importance of the Nucleotide Loop Moiety Corrdinated to the Cobalt Atom of Adenosylcobalamin for Coenzymic Function in the Diol Dehydrase Reaction., THE JOURNAL OF VITAMINOLOGY, 39(2), 115-125, 1993
  82. Inactivation of Ca2+/Calmodulin-Dependent Protein Kinase II by Ca2+/Calmodulin., J Biochem (Tokyo), 115(6), 1075-1082, 1994
  83. A New Peptide Conjugate as a Highly Specific Substrate for MAP Kinase, Journal of Biochemistry, 122(1), 168-172, 19970701
  84. Assay of protein phosphatase activities with phosphopeptide-magnetic bead conjugates, 70(11), 1360-1363, 19981125
  85. Studies on novel protein phosphatases using synthetic peptides, 61(10), 677-680, 20031001
  86. High Level Expression and Preparation of Autonomous Ca^<2+>/Calmodulin-Dependent Protein Kinase II in Escherichia coli, Journal of Biochemistry, 138(5), 605-611, 20051101
  87. Protein phosphatases that regulate multifunctional calmodulin-dependent protein kinases, Technical bulletin of Faculty of Agriculture, Kagawa University, 56, 29-42, 20040315

Patented

  1. Patent, JP5105348, 2012/10/12
  2. Patent, JP4203628, 2008/10/24
  3. Patent, JP4235708, 2008/12/26
  4. Patent, JP4340749, 2009/07/17
  5. Patent, JP4956816, 2012/03/30
  6. Patent, JP2962755, 1999/08/06
  7. Patent, JP2963986, 1999/08/13
  8. Patent, JP3066502, 2000/05/19
  9. Patent, 5,405,839, 1995/04/11, Vitamin B12 derivatives , preparation process thereof, and use thereof

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2018, 2020
  2. KAKENHI, 2015, 2017
  3. KAKENHI, 2013, 2015
  4. Adaptable and Seamless Technology transfer Program through targetdriven R&D, 2013/08/01, 2014/03/31
  5. 2011
  6. KAKENHI, Protective effect of neurosteroids on developmental brains, 2010, 2012
  7. KAKENHI, Method for Pin-Point Structure Analysis of Proteins by Fluorescence-Detected CD, 2010, 2012
  8. KAKENHI, Mechanisms for switching off of CaM-kinases and their involvement in regulation of the cellular phosphorylation balance, 2009, 2011
  9. 2007
  10. KAKENHI, Analysis of Signal Transduction Pathway of Syk in Breast Cancer Cells-as a comparison with that of Her2, 2005, 2006
  11. KAKENHI, A functional site of the essential light chain involved in the regulation of filament assembly of smooth muscle myosin., 2004, 2005
  12. KAKENHI, Enzymatic study of CaM kinase phosphatase for elucidation of its biological function, 2003, 2005
  13. KAKENHI, Studies on Regulatory Mechanisms of CaM-dependent protein kinase phosphatases that dephosphorylate multifunctional CaM-dependent protein kinases, 2000, 2002
  14. KAKENHI, 2000, 2001
  15. KAKENHI, Signal Transduction Mediated by Multifunctional Ca^<2+>/calmodulin-dependent Protein Kinases, 1998, 2001
  16. KAKENHI, 1997, 1998
  17. KAKENHI, 1996, 1998
  18. KAKENHI, 1996, 1996
  19. KAKENHI, Development of methods for detection of various protein kinases in sodium dodecyl sulfate-polyacrylamide gel, 1996, 1997
  20. KAKENHI, 1995, 1995
  21. KAKENHI, 1995, 1995
  22. KAKENHI, 1994, 1994
  23. KAKENHI, Regulation of the Function of CNS by Ca^<2+>/calmodulin-dependent Multifunctional Protein Kinases, 1992, 1994
  24. KAKENHI, Structure and Function of Calmodulin-dependent Protein Kinase II and Its Physiological Significance, 1989, 1991