ATSUHIKO ISHIDA

Last Updated :2024/10/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
http://home.hiroshima-u.ac.jp/ishiyasu/
E-mail
aishidahiroshima-u.ac.jp
Other Contact Details
1-7-1，Kagamiyama，Higashi-Hiroshima, Japan
TEL : (+81) FAX : (+81)
Self-introduction
I am interested in intracellular signal transduction, especially protein phosphorylation and dephosphorylation. I am now studying about multifunctional CaM kinases, which play important roles in Ca2+ signaling, and CaM kinase phosphatase (CaMKP/PPM1F/POPX2), which is involved in their regulation. CaMKP has been reported to be involved in pathogenesis of various diseases such as cancer and depression.

Basic Information

Major Professional Backgrounds

  • 1989/04/01, 1990/02/28, Okayama University
  • 1990/03/01, 2007/03/31, Asahikawa Medical College, Faculty of Medicine, Research Associate
  • 2007/04/01, 2014/03/31, Hiroshima University, Graduate School of Integrated Arts and Sciences, Associate Professor
  • 2014/04/01, 2019/03/31, Hiroshima University, Graduate School of Integrated Arts and Sciences, Professor

Academic Degrees

  • Doctor of Engineering, Kyoto University
  • Master of Engineering, Kyoto University

Educational Activity

  • [Bachelor Degree Program] School of Integrated Arts and Sciences : Department of Integrated Arts and Sciences : Integrated Arts and Sciences
  • [Master's Program] Graduate School of Humanities and Social Sciences : Division of Humanities and Social Sciences : Integrated Arts and Human Sciences Program
  • [Master's Program] Graduate School of Advanced Science and Engineering : Division of Advanced Science and Engineering : Transdisciplinary Science and Engineering Program
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Life and Environmental Sciences
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Humanities and Social Sciences : Division of Humanities and Social Sciences : Integrated Arts and Human Sciences Program
  • [Doctoral Program] Graduate School of Advanced Science and Engineering : Division of Advanced Science and Engineering : Transdisciplinary Science and Engineering Program
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Life and Environmental Sciences
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biomedical Science

In Charge of Primary Major Programs

  • Integrated Arts and Sciences

Research Fields

  • Complex systems;Biomolecular science;Chemical biology
  • Complex systems;Biomolecular science;Biomolecular chemistry
  • Biology;Biological Science;Structural biochemistry
  • Biology;Biological Science;Functional biochemistry

Research Keywords

  • enzyme
  • protein kinase
  • protein phosphatase
  • phosphorylation/dephosphorylation
  • synaptic vesicle
  • bioanalytical chemistry

Affiliated Academic Societies

  • Japanese Biochemical Society
    Related URL
  • Society for neuroscience
  • Japanese Association for Protein Phosphatase Research

Educational Activity

Course in Charge

  1. 2024, Liberal Arts Education Program1, 4Term, Enzymes supporting our life-Introduction to life science-
  2. 2024, Liberal Arts Education Program1, 3Term, Experimental Methods and Laboratory Work in Chemistry I
  3. 2024, Liberal Arts Education Program1, 4Term, Experimental Methods and Laboratory Work in Chemistry II
  4. 2024, Undergraduate Education, 3Term, Seminar in English for Life Sciences
  5. 2024, Undergraduate Education, 2Term, Introduction to Life Sciences
  6. 2024, Undergraduate Education, 2Term, Functional Biochemistry I
  7. 2024, Undergraduate Education, 3Term, Functional Biochemistry II
  8. 2024, Undergraduate Education, 1Term, Laboratory Work in Life Sciences C
  9. 2024, Undergraduate Education, 1Term, Practical Methods in Life Sciences C
  10. 2024, Undergraduate Education, 1Term, Fundamentals of Life Science
  11. 2024, Undergraduate Education, 1Term, Fundamentals of Biochemistry I
  12. 2024, Undergraduate Education, 1Term, Fundamentals of Biochemistry II
  13. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Life and Environmental Sciences
  14. 2024, Graduate Education (Master's Program) , First Semester, Exercises in Life and Environmental Sciences A
  15. 2024, Graduate Education (Master's Program) , Second Semester, Exercises in Life and Environmental Sciences B
  16. 2024, Graduate Education (Master's Program) , 1Term, Introduction to Integrated Arts and Sciences
  17. 2024, Graduate Education (Doctoral Program) , 4Term, Exercises in Integrated Life Sciences
  18. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  19. 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  20. 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
  21. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  22. 2024, Graduate Education (Master's Program) , First Semester, Exercises in Biomedical Science A
  23. 2024, Graduate Education (Master's Program) , Second Semester, Exercises in Biomedical Science B
  24. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  25. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  26. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
  27. 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E

Research Activities

Academic Papers

  1. ★, Functional regulation of the protein phosphatase PPM1M by phosphorylation at multiple sites with Ser/Thr-Pro motifs., Arch Biochem Biophys., 753, 109887, 202403
  2. ★, Diverse physiological functions of CaMK phosphatase and studies on its inhibitors, SEIKAGAKU, 95(5), 676-681, 20231001
  3. ★, Subcellular distribution of bone morphogenetic protein 2-inducible kinase (BMP2K): Regulation by liquid-liquid phase separation and nucleocytoplasmic shuttling, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 649, 16-24, 20230315
    Link to Paper Search Results by DOI
  4. ★, CaMK phosphatase (CaMKP/POPX2/PPM1F) inhibitors suppress the migration of human breast cancer MDA-MB-231 cells with loss of polarized morphology, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 639, 1-8, 20230108
    Link to Paper Search Results by DOI
  5. ★, CaM kinase phosphatase (CaMKP/PPM1F/POPX2) is specifically inactivated through gallate-mediated protein carbonylation., Arch Biochem Biophys, 720, 109170, 20220501
  6. ★, Biochemical characterization of four splice variants of mouse Ca2+/calmodulin-dependent protein kinase Idelta, Journal of Biochemistry, 169(4), 445-458, 20210429
  7. Contribution of DHA diols (19,20-DHDP) produced by cytochrome P450s and soluble epoxide hydrolase to the beneficial effects of DHA supplementation in the brains of rotenone-induced rat models of Parkinson's disease, Biochim. Biophys. Acta - Molecular and Cell Biology of Lipids, 1866(2), 158858, 20210501
    Link to Paper Search Results by DOI
  8. Long noncoding RNA CCDC26 as a modulator of transcriptional switching between fetal and embryonic globins, Biochim. Biophys. Acta - Molecular Cell Research, 1868, 118931, 20210401
  9. ★, Dual phosphorylation of protein phosphatase PPM1H promotes dephosphorylation of Smad1 in cellulo, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 530(3), 513-519, 20200924
    Link to Paper Search Results by DOI
  10. ★, Autoactivation of C-terminally truncated Ca2+/calmodulin-dependent protein kinase (CaMK) Idelta via CaMK kinase-independent autophosphorylation., Arch Biochem Biophys, 668, 29-38, 20190507
  11. ★, Functions and dysfunctions of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) and CaMKP-N/PPM1E., Archives of biochemistry and biophysics, 640, 83-92, 20180215
  12. Progression of vasogenic edema induced by activated microglia under permanent middle cerebral artery occlusion., Biochemical and biophysical research communications, 496(2), 582-587, 20180117
  13. ★, Facile preparation of highly active casein kinase 1 using Escherichia coli constitutively expressing lambda phosphatase., Analytical biochemistry, 549, 99-106, 20180317
  14. Characterization of CoPK02, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea., Bioscience, biotechnology, and biochemistry, 82(8), 1335-1343, 20180420
  15. ★, High-performance CaMKI: A highly active and stable form of CaMKI delta produced by high-level soluble expression in Escherichia coli, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 475(3), 277-282, 20160701
    Link to Paper Search Results by DOI
  16. ★, Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) interacts with neurofilament L and inhibits its filament association, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 477(4), 820-825, 20160902
    Link to Paper Search Results by DOI
  17. Effects of sex steroid hormones and their metabolites on neuronal injury caused by oxygen-glucose deprivation/reoxygenation in organotypic hippocampal slice cultures, STEROIDS, 113, 71-77, 20160902
    Link to Paper Search Results by DOI
  18. Selective estrogen-receptor modulators suppress microglial activation and neuronal cell death via an estrogen receptor-dependent pathway, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 145, 85-93, 201504
    Link to Paper Search Results by DOI
  19. Protective Actions of 17 beta-Estradiol and Progesterone on Oxidative Neuronal Injury Induced by Organometallic Compounds, OXIDATIVE MEDICINE AND CELLULAR LONGEVITY, 201504
    Link to Paper Search Results by DOI
  20. Long noncoding RNA, CCDC26, controls myeloid leukemia cell growth through regulation of KIT expression, MOLECULAR CANCER, 14, 201504
    Link to Paper Search Results by DOI
  21. ★, The phosphatase-resistant isoform of CaMKI, Ca2+/calmodulin-dependent protein kinase Id (CaMKId), remains in its 'Primed' form without Ca2+stimulation., Biochemistry, 54, 3617-3630, 201504
  22. Neuroprotection elicited by nerve growth factor and brain-derived neurotrophic factor released from astrocytes in response to methylmercury, ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY, 40(1), 199-205, 201504
    Link to Paper Search Results by DOI
  23. Dual Role of Superoxide Dismutase 2 Induced in Activated Microglia OXIDATIVE STRESS TOLERANCE AND CONVERGENCE OF INFLAMMATORY RESPONSES, JOURNAL OF BIOLOGICAL CHEMISTRY, 290(37), 22805-22817, 201504
    Link to Paper Search Results by DOI
  24. ★, Regulation of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP/PPM1F) by protocadherin-gamma C5 (Pcdh-gamma C5), ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 585, 109-120, 201504
    Link to Paper Search Results by DOI
  25. Suppressive effects of 17 beta-estradiol on tributyltin-induced neuronal injury via Akt activation and subsequent attenuation of oxidative stress, LIFE SCIENCES, 99(1-2), 24-30, 20140318
    Link to Paper Search Results by DOI
  26. De Novo Synthesized Estradiol Protects against Methylmercury-Induced Neurotoxicity in Cultured Rat Hippocampal Slices, PLOS ONE, 8(2), 20130206
    Link to Paper Search Results by DOI
  27. Allopregnanolone-mediated protective effects of progesterone on tributyltin-induced neuronal injury in rat hippocampal slices, JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY, 135, 1-6, 20130501
    Link to Paper Search Results by DOI
  28. ★, Ink-native electrophoresis: An alternative to blue-native electrophoresis more suitable for in-gel detection of enzymatic activity, ANALYTICAL BIOCHEMISTRY, 440(2), 142-144, 20130915
    Link to Paper Search Results by DOI
  29. ★, An Active C-Terminally Truncated Form of Ca2+/Calmodulin-Dependent Protein Kinase Phosphatase-N (CaMKP-N/PPM1E), BIOMED RESEARCH INTERNATIONAL, 2013
    Link to Paper Search Results by DOI
  30. Functional processing of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N): Evidence for a critical role of proteolytic processing in the regulation of its catalytic activity, subcellular localization and substrate targeting in vivo, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 517(1), 43-52, 20120101
    Link to Paper Search Results by DOI
  31. ★, Synaptic vesicles are capable of synthesizing the VGLUT substrate glutamate from a-ketoglutarate for vesicular loading, JOURNAL OF NEUROCHEMISTRY, 121(2), 184-196, 201204
    Link to Paper Search Results by DOI
  32. Tributyltin induces oxidative stress and neuronal injury by inhibiting glutathione S-transferase in rat organotypic hippocampal slice cultures, NEUROCHEMISTRY INTERNATIONAL, 60(8), 782-790, 201206
    Link to Paper Search Results by DOI
  33. Phosphorylation and activation of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N/PPM1E) by Ca2+/calmodulin-dependent protein kinase I (CaMKI), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 422(4), 703-709, 20120615
    Link to Paper Search Results by DOI
  34. Regulation of Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP) by oxidation/reduction at Cys-359, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 526(1), 9-15, 20121001
    Link to Paper Search Results by DOI
  35. ★, A sensitive method based on fluorescence-detected circular dichroism for protein local structure analysis, ANALYTICAL BIOCHEMISTRY, 430(2), 179-184, 20121115
    Link to Paper Search Results by DOI
  36. Environmental enrichment alters gene expression of steroidogenic enzymes in the rat hippocampus., Gen Comp Endocrinol., 171(1), 28-32, 20110301
  37. CoPK32 is a novel stress-responsive protein kinase in the mushroom Coprinopsis cinerea., Biochim Biophys Acta., 1810, 620-629, 20110401
  38. ★, In-Gel Protein Phosphatase Assays and Other Useful Methods for the Detection of Protein Phosphatase Activities, ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY, 11(1), 47-53, 201101
    Link to Paper Search Results by DOI
  39. A minimum size homologue of Ca2+/calmodulin-dependent protein kinase IV naturally occurring in zebrafish, JOURNAL OF BIOCHEMISTRY, 147(6), 857-865, 201006
    Link to Paper Search Results by DOI
  40. Oxazolone-induced over-expression of focal adhesion kinase in colonic epithelial cells of colitis mouse model, FEBS LETTERS, 584(18), 3949-3954, 20100924
    Link to Paper Search Results by DOI
  41. ★, Synaptic Vesicle-bound Pyruvate Kinase can Support Vesicular Glutamate Uptake, Neurochem Res, 34(5), 807-818, 20090501
  42. Social isolation stimulates hippocampal estradiol syntheses, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 379(2), 480-484, 20090206
    Link to Paper Search Results by DOI
  43. Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP) is indispensable for normal embryogenesis in zebrafish, Danio rerio, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 488(1), 48-59, 20090801
    Link to Paper Search Results by DOI
  44. Retinoic Acid Stimulates 17 beta-Estradiol and Testosterone Synthesis in Rat Hippocampal Slice Cultures, ENDOCRINOLOGY, 150(9), 4260-4269, 200909
    Link to Paper Search Results by DOI
  45. ★, Negative regulation of multifunctional Ca(2+)/calmodulin-dependent protein kinases: physiological and pharmacological significance of protein phosphatases, BRITISH JOURNAL OF PHARMACOLOGY, 154(4), 729-740, 200806
    Link to Paper Search Results by DOI
  46. Tyrosine kinase activity of a Ca(2+)/calmodulin-dependent protein kinase II catalytic fragment, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 377(2), 648-652, 20081212
    Link to Paper Search Results by DOI
  47. Knockdown of nuclear Ca2+/calmodulin-dependent protein kinase phosphatase causes developmental abnormalities in zebrafish., Arch Biochem Biophys, 457, 205-216, 20070401
  48. ★, Recent advances in technologies to analyze protein kinases., J Pharmacol Sci, 103, 5-11, 20070101
  49. ★, Inhibitors of the Ca2+/calmodulin-dependent protein kinase phosphatase family (CaMKP and CaMKP-N), BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 363(3), 715-721, 20071123
    Link to Paper Search Results by DOI
  50. Synthesis of caged peptides using caged lysine: Application to the synthesis of caged AIP, a highly specific inhibitor of calmodulin-dependent protein kinase II, BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 9(8), 1093-1096, 1999
  51. Phosphorylation and activation of Ca2+/calmodulin-dependent protein kinase phosphatase by Ca2+/calmodulin-dependent protein kinase II, FEBS LETTERS, 456(2), 249-252, 1999
  52. A novel protein phosphatase that dephosphorylates and regulates Ca2+/calmodulin-dependent protein kinase II, JOURNAL OF BIOLOGICAL CHEMISTRY, 273(4), 1904-1910, 1998
  53. Critical amino acid residues of AIP, a highly specific inhibitory peptide of calmodulin-dependent protein kinase II, FEBS LETTERS, 427(1), 115-118, 1998
  54. Analysis of protein-protein interaction by two-dimensional affinity electrophoresis, ANALYTICAL BIOCHEMISTRY, 262(1), 90-92, 1998
  55. Assay of protein phosphatase activities with phosphopeptide-magnetic bead conjugates, SEIKAGAKU, 70(11), 1360-1363, 1998
  56. Regulation of multifunctional Ca2+/calmodulin-dependent protein kinases by Ca2+/calmodulin-dependent protein kinase phosphatase, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 253(1), 159-163, 1998
  57. Assay of protein phosphatase activities with phosphopeptide-magnetic particle conjugates, ANALYTICAL BIOCHEMISTRY, 254(1), 152-155, 1997
  58. A new peptide conjugate as a highly specific substrate for MAP kinase, JOURNAL OF BIOCHEMISTRY, 122(1), 168-172, 1997
  59. Detection of protein phosphatase activities in sodium dodecyl sulfate-polyacrylamide gel using peptide substrates, ANALYTICAL BIOCHEMISTRY, 245(2), 149-153, 1997
  60. Calcium- and calmodulin-dependent phosphorylation of AMPA type glutamate receptor subunits by endogenous protein kinases in the post-synaptic density, MOLECULAR BRAIN RESEARCH, 46(1-2), 338-342, 1997
  61. Evidence that autophosphorylation at Thr-286/Thr-287 is required for full activation of calmodulin-dependent protein kinase II, BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH, 1311(3), 211-217, 1996
  62. NOVEL HIGHLY SPECIFIC AND POTENT INHIBITOR OF CALMODULIN-DEPENDENT PROTEIN-KINASE-II, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 212(3), 806-812, 1995
  63. STABILIZATION OF CALMODULIN-DEPENDENT PROTEIN-KINASE-II THROUGH THE AUTOINHIBITORY DOMAIN, JOURNAL OF BIOLOGICAL CHEMISTRY, 270(5), 2163-2170, 1995
  64. Requirement of calmodulin-dependent protein kinase II in cyclic ADP-ribose-mediated intracellular Ca2+ mobilization, JOURNAL OF BIOLOGICAL CHEMISTRY, 270(51), 30257-30259, 1995
  65. PHOSPHORYLATION OF RABPHILIN-3A BY CALMODULIN-DEPENDENT PROTEIN-KINASE-II, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 205(3), 1776-1784, 1994
  66. MICROBIOLOGICAL ACTIVITIES OF NUCLEOTIDE LOOP-MODIFIED ANALOGS OF VITAMIN-B-12, ARCHIVES OF MICROBIOLOGY, 161(4), 293-299, 1994
  67. ADENOSYLCOBINAMIDE METHYL PHOSPHATE AS A PSEUDOCOENZYME FOR DIOL DEHYDRASE, BIOCHEMISTRY, 32(6), 1535-1540, 1993
  68. ROLES OF THE D-RIBOSE AND 5,6-DIMETHYLBENZIMIDAZOLE MOIETIES OF THE NUCLEOTIDE LOOP OF ADENOSYLCOBALAMIN IN MANIFESTATION OF COENZYMIC FUNCTION IN THE DIOL DEHYDRASE REACTION, JOURNAL OF BIOLOGICAL CHEMISTRY, 266(9), 5430-5437, 1991
  69. ACCELERATION OF CLEAVAGE OF THE CARBON COBALT BOND OF STERICALLY HINDERED ALKYLCOBALAMINS BY BINDING TO APOPROTEIN OF DIOL DEHYDRASE, BIOCHEMISTRY, 27(20), 7677-7681, 1988
  70. Cellular localization of CoPK12, a Ca2+/calmodulin-dependent protein kinase in mushroom Coprinopsis cinerea, is regulated by N-myristoylation, JOURNAL OF BIOCHEMISTRY, 156(1), 51-61, 2014
  71. ★, In-gel kinase assay revisited, SEIBUTSU BUTSURI KAGAKU, 58(2), 62-64, 2014
  72. Effect of housing conditions on neurotoxicity of trimethyltin in rat brain, NEUROSCIENCE RESEARCH, 68, E258-E258, 2010
  73. Social isolation increases mRNA contents of estradiol synthetic enzymes, NEUROSCIENCE RESEARCH, 61, S187-S187, 2008
  74. ENVIRONMENTAL ENRICHMENT INCREASES MRNA CONTENTS OF ALLOPREGNANOLONE SYNTHETIC ENZYMES, JOURNAL OF PHYSIOLOGICAL SCIENCES, 59, 293-293, 2009
  75. Neuroprotective effect of estradiol on methylmercury-induced neuronal cell toxicity in rat hippocampal slices, NEUROSCIENCE RESEARCH, 65, S220-S220, 2009
  76. Evaluation of neurotoxicity of trimethyltin in rat brain under various housing conditions, NEUROSCIENCE RESEARCH, 71, E340-E340, 2011
  77. Identification and Characterization of CaMKP-N, Nuclear Calmodulin-Dependent Protein Kinase Phosphatase, Journal of Biochemistry, 130(6), 833-840, 20011201
  78. EPR Spectroscopic Evidence for the Mechanism Based Inactivation of Adenosylcobalamin-Dependent Diol Dehydratase by Coenzyme Analogs., J Biochem (Tokyo), 124(3), 598-601, 1998
  79. Detection of a Variety of Ser/Thr Protein Kinases Using a Synthetic Peptide with Multiple Phosphorylation Sites., J Biochem (Tokyo), 126(6), 991-995, 1999
  80. Substrate Specificity of Ca2+/Calmodulin-Dependent Protein Kinase Phosphatase: Kinetic Studies Using Synthetic Phosphopeptides as Model Substrates., J Biochem (Tokyo), 129(5), 745-753, 2001
  81. ★, Identification of major Ca^<2+>/calmodulin-dependent protein kinase phosphatase-binding proteins in brain. Biochemical analysis of the interaction., ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 435(1), 134-146, 200503
  82. Generation of a polyclonal antibody that simultaneously detects multiple Ser/Thr protein kinases, JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS, 60(1), 13-22, 200407
  83. ★, Stimulation of Ca^<2+>/calmodulin-dependent protein kinase phosphatase by polycations, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 408(2), 229-238, 200212
  84. ★, Phosphorylation of calmodulin by Ca2+/calmodulin-dependent protein kinase IV, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 407(1), 72-82, 200211
  85. ★, Protein phosphatases that regulate multifunctional Ca2+/calmodulin-dependent protein kinases: from biochemistry to pharmacology, Pharmacology and Therapeutics, 100(3), 291-305, 200312
  86. Importance of the Nucleotide Loop Moiety Corrdinated to the Cobalt Atom of Adenosylcobalamin for Coenzymic Function in the Diol Dehydrase Reaction., THE JOURNAL OF VITAMINOLOGY, 39(2), 115-125, 1993
  87. Inactivation of Ca2+/Calmodulin-Dependent Protein Kinase II by Ca2+/Calmodulin., J Biochem (Tokyo), 115(6), 1075-1082, 1994
  88. A New Peptide Conjugate as a Highly Specific Substrate for MAP Kinase, Journal of Biochemistry, 122(1), 168-172, 19970701
  89. Assay of protein phosphatase activities with phosphopeptide-magnetic bead conjugates, 70(11), 1360-1363, 19981125
  90. Studies on novel protein phosphatases using synthetic peptides, 61(10), 677-680, 20031001
  91. High Level Expression and Preparation of Autonomous Ca^<2+>/Calmodulin-Dependent Protein Kinase II in Escherichia coli, Journal of Biochemistry, 138(5), 605-611, 20051101
  92. Protein phosphatases that regulate multifunctional calmodulin-dependent protein kinases, Technical bulletin of Faculty of Agriculture, Kagawa University, 56, 29-42, 20040315

Invited Lecture, Oral Presentation, Poster Presentation

  1. Natural polyphenol compounds with pyrogallol structure specifically inactivate CaM kinase phosphatase (CaMKP/PPM1F/POPX2) by protein carbonylation, Kazutoshi Akizuki, Hana Ozaki, Rika Obatake, Shun Ishikawa, Tatsuo Nehira, Yasushi Shigeri, Noriyuki Sueyoshi, Toshiyuki Murai, Atsuhiko Ishida, 2024/06/02, Without Invitation, English
  2. Specific inactivation of CaM kinase phosphatase (CaMKP/PPM1F/POPX2) through carbonylation mediated by gallate derivatives, Atsuhiko Ishida, Japan-Singapore Advanced Cancer Research Online International Symposium, 2022/01/25, With Invitation, English, Wakayama Medical University, On-line

Patented

  1. Patent, JP5105348, 2012/10/12
  2. Patent, JP4203628, 2008/10/24
  3. Patent, JP4235708, 2008/12/26
  4. Patent, JP4340749, 2009/07/17
  5. Patent, JP4956816, 2012/03/30
  6. Patent, JP2962755, 1999/08/06
  7. Patent, JP2963986, 1999/08/13
  8. Patent, JP3066502, 2000/05/19
  9. Patent, 5,405,839, 1995/04/11, Vitamin B12 derivatives , preparation process thereof, and use thereof

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2021, 2023
  2. 2021/05/01, 2022/09/30
  3. 2021/05/01, 2022/03/31
  4. 2019/01/01, 2020/12/31
  5. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2018, 2020
  6. KAKENHI, 2015, 2017
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  7. Adaptable and Seamless Technology transfer Program through targetdriven R&D, 2013/08/01, 2014/03/31
  8. 2011
  9. KAKENHI, Mechanisms for switching off of CaM-kinases and their involvement in regulation of the cellular phosphorylation balance, 2009, 2011
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  10. 2007
  11. KAKENHI, Enzymatic study of CaM kinase phosphatase for elucidation of its biological function, 2003, 2005
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  12. KAKENHI, Studies on Regulatory Mechanisms of CaM-dependent protein kinase phosphatases that dephosphorylate multifunctional CaM-dependent protein kinases, 2000, 2002
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  13. KAKENHI, 2000, 2001
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  14. KAKENHI, 1997, 1998
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  15. KAKENHI, 1996, 1996
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  16. KAKENHI, 1995, 1995
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  17. KAKENHI, 1994, 1994
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