TAKESHI IGAWA

Last Updated :2024/02/01

Affiliations, Positions
Amphibian Research Center, Associate Professor
E-mail
tigawahiroshima-u.ac.jp
Self-introduction
My interest is identifying evolutionary dynamics in genes and genomes in amphibian species. I started my career from phylogenetics and shifted to population genetics and molecular ecology.

Basic Information

Major Professional Backgrounds

  • 2008/04/01, 2009/03/31, The Graduate University for Advanced Studies
  • 2009/04/01, 2014/03/31, Hiroshima University, Graduate School of Science, Institute for Amphibian Biology, Assistant Professor (Special Appointment)
  • 2014/04/01, 2017/04/30, Hiroshima University, Graduate School for International Development and Cooperation, Assistant Professor
  • 2017/05/01, 2022/09/30, Hiroshima University, Amphibian Research Center, Assistant Professor
  • 2022/10/01, Hiroshima University, Amphibian Research Center, Associate Professor

Educational Backgrounds

  • Hiroshima University, Japan, 2005/04, 2008/03
  • Hiroshima University, Japan, 2003/04, 2005/03
  • Shimane University, Japan, 1999/04, 2003/03

Academic Degrees

  • Doctor of Science, Hiroshima University
  • Master of Science, Hiroshima University

Research Fields

  • Biology;Basic biology;Evolutionary biology
  • Biology;Basic biology;Genetics / Chromosome dynamics
  • Biological Sciences;Conservation of biological resources;Conservation of biological resources

Research Keywords

  • Mitochondrial genome, Microsatellite, Genomic Evolution, Molecular Evolution, Evolutionary Biology, Phylogeography, Population Genetics, Landscape Genetics, Conservation Genetics

Affiliated Academic Societies

  • The Herpetological Society of Japan, 2003/04
  • Zoological Society of Japan, 2003/04
  • The Genetics Society of Japan, 2006/04
  • Society for Evolutionary Studies, Japan, 2008/04
  • The Okinawa Herpetological Society, 2011/04
  • NGS Field, 2012/04

Educational Activity

Course in Charge

  1. 2023, Liberal Arts Education Program1, 1Term, Environmental management in higash-hiroshima campus at Hiroshima University
  2. 2023, Liberal Arts Education Program1, 3Term, The life science of amphibians
  3. 2023, Liberal Arts Education Program1, 2Term, Introductory Seminar for First-Year Students
  4. 2023, Undergraduate Education, 2Term, Advanced Biology
  5. 2023, Undergraduate Education, 3Term, Regulation of Animal Morphology
  6. 2023, Undergraduate Education, Second Semester, Special Study for Graduation
  7. 2023, Undergraduate Education, Second Semester, Seminar for Amphibian Biology
  8. 2023, Undergraduate Education, First Semester, Practice for Fundamental Biology I
  9. 2023, Undergraduate Education, Second Semester, Practice for Fundamental Biology II
  10. 2023, Undergraduate Education, Second Semester, Practice for Fundamental Biology IV
  11. 2023, Undergraduate Education, 3Term, Laboratory Work in Biology A
  12. 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Basic Biology
  13. 2023, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
  14. 2023, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
  15. 2023, Graduate Education (Master's Program) , 1Term, Advanced Technologies for Life Science
  16. 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  17. 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biomedial Science
  18. 2023, Graduate Education (Master's Program) , First Semester, Exercises in Biomedical Science A
  19. 2023, Graduate Education (Master's Program) , Second Semester, Exercises in Biomedical Science B
  20. 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
  21. 2023, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  22. 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
  23. 2023, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E

Research Activities

Academic Papers

  1. Environmental DNA methods for detecting two invasive alien species (American bullfrog and red swamp crayfish) in Japanese ponds, Ecology Research, 2022, 1-10, 20220705
  2. Invention sharing is the mother of developmental biology., Development, growth & differentiation, 63(8), 20211028
  3. Invention sharing is the mother of developmental biology (Part 2)., Development, growth & differentiation, 63(9), 20211231
  4. Invention sharing is the mother of developmental biology (Part 3)., Development, growth & differentiation, 64(1), 20220131
  5. Evolutionary Tuning of Transient Receptor Potential Ankyrin 1 Underlies the Variation in Heat Avoidance Behaviors among Frog Species Inhabiting Diverse Thermal Niches, MOLECULAR BIOLOGY AND EVOLUTION, 39(9), 20220901
  6. Crystal structure of thermally stable homodimeric cytochrome c '-beta from Thermus thermophilus, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 78, 217-225, 20220601
  7. Optimization of CRISPR/Cas9-mediated gene disruption in Xenopus laevis using a phenotypic image analysis technique, DEVELOPMENT GROWTH & DIFFERENTIATION, 64(4), 219-225, 202205
  8. Cryo-injury procedure-induced cardiac regeneration shows unique gene expression profiles in the newt Pleurodeles waltl, DEVELOPMENTAL DYNAMICS, 251(5), 864-876, 202205
  9. Transcriptomic Changes in Hot Spring Frog Tadpoles (Buergeria otai) in Response to Heat Stress, FRONTIERS IN ECOLOGY AND EVOLUTION, 9, 20211013
  10. Spontaneous neoplasia in the western clawed frog Xenopus tropicalis, microPublication Biology, 20200831
  11. Heterogeneity of synonymous substitution rates in the Xenopus frog genome, PLoS One, 15(8), 20200807
  12. Complete mitochondrial genome of Hynobius dunni (Amphibia, Caudata, Hynobiidae) and its phylogenetic position, Mitochondrial DNA Part B-Resources, 5(3), 2241-2242, 20200702
  13. Comparison of the detection of 3 endangered frog species by eDNA and acoustic surveys across 3 seasons, Freshwater Science, 39(1), 18-27, 20200301
  14. Expression Changes of MHC and Other Immune Genes in Frog Skin during Ontogeny., Animals, 10(1), 20200106
  15. ★, Detecting inter- and intra-island genetic diversity: population structure of the endangered crocodile newt, Echinotriton andersoni, in the Ryukyus, Conservation Genetics, 21(1), 13-26, 202002
  16. An application of PCR-RFLP species identification assay for environmental DNA detection, PeerJ, 7, 20191003
  17. Transgenic, Inbred and Mutant Animals, Training Opportunities, and Web-Based Support., Frontiers in Physiology, 10, 387, 201904
  18. Quantitative trait loci mapping for the shear force value in breast muscle of F-2 chickens, Poultry Science, 98(3), 1096-1101, 201903
  19. Selective constraint acting on TLR2 and TLR4 genes of Japanese Rana frogs, PEERJ, 6, 20180525
  20. Transcriptome analyses of immune tissues from three Japanese frogs (genus Rana) reveals their utility in characterizing major histocompatibility complex class II, BMC Genomics, 18, 20171228
  21. ★, Fine-scale demographic processes resulting from multiple overseas colonization events of the Japanese stream tree frog, Buergeria japonica., Journal of Biogeography, 44, 1586-1597, 201707
  22. Evolutionary history of the extant amphioxus lineage with shallow-branching diversification, Scientific Reports, 7, 1157, 201704
  23. Development and Characterization of 23 Microsatellite Markers for the Montane Brown Frog (Rana ornativentris), Current Herpetology, 36, 63-68, 201702
  24. Salinity and thermal tolerance of Japanese stream tree frog (Buergeria japonica) tadpoles from island populations., Herpetological Journal, 26, 207-211, 20160701
  25. Population structure and evolution after speciation of the Hokkaido salamander (Hynobius retardatus), PLoS One, 11(6), e0156815, 20160603
  26. The first see-through frog created by breeding: description, inheritance patterns, and dermal chromatophore structure., Scientific Reports, 6, 24431, 20160415
  27. Characterisation of major histocompatibility complex class I genes in Japanese Ranidae frogs, Immunogenetics, 68(10), 797-806, 201611
  28. Inter- and intra-specific genetic divergence of Asian tiger frogs (genus Hoplobatrachus), with special reference to the population structure of H. tigerinus in Bangladesh, Genes & Genetic Systems, 91, 217-228, 201608
  29. Living in a Japanese onsen: field observations and physiological measurements of hot spring amphibian tadpoles, Buergeria japonica, AMPHIBIA-REPTILIA, 37(3), 311-314, 2016
  30. Robust molecular phylogeny and palaeodistribution modelling resolve a complex evolutionary history: glacial cycling drove recurrent mtDNA introgression among Pelophylax frogs in East Asia, JOURNAL OF BIOGEOGRAPHY, 42(11), 2159-2171, 201511
  31. Genetic population structure and demographic history of an endangered frog, Babina holsti, Conservation Genetics, 16(4), 987-1000, 201508
  32. Development and characterization of 12 microsatellite markers for the Hokkaido salamander (Hynobius retardatus), Current Herpetology, 34, 177-181, 201508
  33. ★, Inbreeding ratio and genetic relationships among strains of the Western clawed frog, Xenopus tropicalis, PLoS One, 10(7), e0133963, 201507
  34. Radiocesium accumulation in the anuran frog, Rana tagoi tagoi, in forest ecosystems after the Fukushima Nuclear Power Plant accident, ENVIRONMENTAL POLLUTION, 199, 89-94, 201504
  35. Development and Validation of PCR-RFLP Assay to Identify Three Japanese Brown Frogs of The True Frog Genus Rana, Current Hepetology, 34, 89-94, 201502
  36. Microsatellite Marker Development by Multiplex Ion Torrent PGM Sequencing: a Case Study of the Endangered Odorrana narina Complex of Frogs, JOURNAL OF HEREDITY, 106(1), 131-137, 2015
  37. Extraordinary Diversity in the Origins of Sex Chromosomes in Anurans Inferred from Comparative Gene Mapping, CYTOGENETIC AND GENOME RESEARCH, 145(3-4), 218-229, 2015
  38. Genetic Divergence and Geographic Distribution of Frogs in Genus Fejervarya from Indonesia Inferred from Mitochondrial 16S rRNA Gene Analysis, Treubia, 41, 1-16, 201412
  39. Development and characterization of 14 microsatellite markers for Buergeria japonica (Amphibia, Anura, Rhacophoridae), GENES & GENETIC SYSTEMS, 89(1), 35-39, 201402
  40. Genetic divergences of South and Southeast Asian frogs: a case study of several taxa based on 16S ribosomal RNA gene data with notes on the generic name Fejervarya, TURKISH JOURNAL OF ZOOLOGY, 38(4), 389-411, 2014
  41. Development and characterization of 27 new microsatellite markers for the Indian bullfrog Hoplobatrachus tigerinus and its congeneric species, GENES & GENETIC SYSTEMS, 89(3), 137-141, 2014
  42. Development and characterization of new microsatellite loci in the Otton frog (Babina subaspera) and cross-amplification in a congeneric species, Holst's frog (B-holsti), CONSERVATION GENETICS RESOURCES, 5(4), 1071-1073, 201312
  43. Stable Aquaculture of the Japanese Lancelet Branchiostoma japonicum for 7 Years, JOURNAL OF EXPERIMENTAL ZOOLOGY PART B-MOLECULAR AND DEVELOPMENTAL EVOLUTION, 320(8), 538-547, 201312
  44. An Attempt at Captive Breeding of the Endangered Newt Echinotriton andersoni, from the Central Ryukyus in Japan, Animals, 3, 680-692, 201307
  45. ★, Population structure and landscape genetics of two endangered frog species of genus Odorrana: different scenarios on two islands, HEREDITY, 110(1), 46-56, 201301
  46. Isolation and characterization of ten microsatellite loci of endangered Anderson's crocodile newt, Echinotriton andersoni, CONSERVATION GENETICS RESOURCES, 4(3), 595-598, 201209
  47. Cryptic Anuran Biodiversity in Bangladesh Revealed by Mitochondrial 16S rRNA Gene Sequences, ZOOLOGICAL SCIENCE, 29(3), 162-172, 201203
  48. Genomic structure and evolution of multigene families: "flowers" on the human genome, International journal of evolutionary biology, 2012(917678), 1-11, 201201
  49. Artificial Production and Natural Breeding of the Endangered Frog Species Odorrana ishikawae, with Special Reference to Fauna Conservation in the Laboratory, ZOOLOGICAL SCIENCE, 28(11), 834-839, 201111
  50. Isolation and characterization of twelve microsatellite loci of endangered Ishikawa's frog (Odorrana ishikawae), CONSERVATION GENETICS RESOURCES, 3(3), 421-424, 201107
  51. ★, Divergence, demography and gene loss along the human lineage., Philosophical transactions of the Royal Society of London. Series B, 365(1552), 2451-2457, 201008
  52. Genetic Divergence and Evolutionary Relationship in Fejervarya cancrivora from Indonesia and Other Asian Countries Inferred from Allozyme and MtDNA Sequence Analyses, ZOOLOGICAL SCIENCE, 27(3), 222-233, 201003
  53. Complete mitochondrial genomes of three neobatrachian anurans: A case study of divergence time estimation using different data and calibration settings, GENE, 407(1-2), 116-129, 20080115
  54. Genetic divergence and evolutionary relationships in six species of genera Hoplobatrachus and Euphlyctis (Amphibia : Anura) from Bangladesh and other Asian countries revealed by mitochondrial gene sequences, MOLECULAR PHYLOGENETICS AND EVOLUTION, 48(2), 515-527, 200808
  55. Evolutionary relationships and reproductive isolating mechanisms in the rice frog (Fejervarya limnocharis) species complex from Sri Lanka, Thailand, Taiwan and Japan, inferred from mtDNA gene sequences, allozymes, and crossing experiments, ZOOLOGICAL SCIENCE, 24(6), 547-562, 200706
  56. Molecular phylogenetic relationship of toads distributed in the Far East and Europe inferred from the nucleotide sequences of mitochondrial DNA genes, MOLECULAR PHYLOGENETICS AND EVOLUTION, 38(1), 250-260, 20060101
  57. Hot spring frogs (Buergeria japonica) prefer cooler water to hot water., Ecology and evolution, 10(17), 9466-9473, 20200811
  58. Geographic Origin and Genetic Characteristics of Japanese Indigenous Chickens Inferred from Mitochondrial D-Loop Region and Microsatellite DNA Markers., Animals : an open access journal from MDPI, 10(11), 2074, 20201109
  59. Mapping of Quantitative Trait Loci for Growth and Carcass-Related Traits in Chickens Using a Restriction-Site Associated DNA Sequencing Method., The journal of poultry science, 56(3), 166-176, 20190725
  60. Heterologous expression and biochemical comparison of two homologous SoxX proteins of endosymbiotic Candidatus Vesicomyosocius okutanii and free-living Hydrogenovibrio crunogenus from deep-sea environments., Protein expression and purification, 200, 20220801

Publications such as books

  1. 2020/10/01, Caudata 04, Breeding and observation of breeding behavior of Crocodile newt, Biodiversity, Crocodile newt, conservation, ex situ conservation, SDGs, Goal 15, Yuubisha, 2020, 10, Scholarly Book, Single work, 日本語, Takeshi Igawa, 84, 18-22

Invited Lecture, Oral Presentation, Poster Presentation

  1. Genetic divergence and phylogenetic relationships in the frogs from south to east Asia, Hasan, M, MM. Islam, MR. Khan, MS. Alam, TH. Djong, T. Igawa, N. Kurniawan, M. Kuramoto, M. Sumida, 2010/05/16, Without Invitation, Japanese
  2. Species diversity and phylogenetic relationships among frogs from Bangladesh revealed by nucleotide sequences of mitochondrial 16S rRNA gene, Hasan, M, MM. Islam, MR. Khan, MS. Alam, T. Igawa, M. Kuramoto, M. Sumida, 2010/09/21, Without Invitation, Japanese

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2021, 2023
  2. KAKENHI(Grant-in-Aid for Scientific Research (C)), 2018, 2020
  3. KAKENHI, 2014, 2016
  4. 光る透明ガエルの作出:非モデル両生類への遺伝子導入法の確立とNGSによる発現解析, 2013, 2015
  5. KAKENHI, 2012, 2015
  6. KAKENHI, 2011, 2013
  7. Sasakawa Scientific Research Grant Award, Molecular Evolutionary Study on Sex-linked gene GOT-1 in Japanese Bell Ring Frog, Buergeria buergeri, 2011, 2012
  8. KAKENHI, Evolution by loss of genes that have become redundant in relation to changing environments, 2004, 2008

Social Activities

History as Committee Members

  1. Planning committee, 2022/10, Chugoku, Shikoku branch of Zoological Society of Japan

Organizing Academic Conferences, etc.

  1. 2008/06, 2008/08
  2. suborganizer, 2018/09, 2018/09
  3. Amphibian development, regeneration, evolution and beyond, suborganizer, 2018/03, 2018/03
  4. Frontiers in Amphibian Biology: Endangered Species Conservation and Genome Editing, general affairs, 2014/, 2014/

History as Peer Reviews of Academic Papers

  1. 2022, Development, Growth & Differentiation, Others, Reviewer, 1
  2. 2022, DNA Research, Others, Reviewer, 2
  3. 2022, Bulletin of the Herpetological Society of Japan, Others, Reviewer, 1
  4. 2021, Development, Growth & Differentiation, Others, Reviewer, 2
  5. 2021, Current Herpetology, Others, Reviewer, 1
  6. 2020, Mammal Study, Others, Reviewer, 2
  7. 2020, Zoological Science, Others, Reviewer, 2
  8. 2020, Molecular Biology and Evolution, Others, Reviewer, 1
  9. 2019, Limnology, Others, Reviewer, 1
  10. 2019, PLoS One, Others, Reviewer, 1
  11. 2019, Current Herpetology, Others, Reviewer, 1
  12. 2022, Frontiers in Genetics, Editor, Guest Editor, 1
  13. 2022, Development, Growth & Differentiation, Editor, Guest Editor, 0
  14. 2021, Frontiers in Genetics, Editor, Guest Editor, 1
  15. 2021, Development, Growth & Differentiation, Editor, Guest Editor, 1
  16. 2020, Animals (Basel), Editor, Guest Editor, 5
  17. 2018, Bulletin of the Herpetological Society of Japan, Others, Reviewer, 1
  18. 2012, Journal of Tropical Life Sciences, Editor, Editor, 0
  19. 2009, Zoological Science, Others, Reviewer, 1
  20. 2014, Ecological Research, Others, Reviewer, 1
  21. 2014, Plos One, Others, Reviewer, 1
  22. 2014, Molecular Phylogenetics and Evolution, Others, Reviewer, 1
  23. 2015, Frontiers in Zoology, Others, Reviewer, 1
  24. 2015, Marine Genomics, Others, Reviewer, 1
  25. 2015, PeerJ, Others, Reviewer, 1
  26. 2015, Plos One, Others, Reviewer, 1
  27. 2015, Zoological Science, Others, Reviewer, 1
  28. 2016, PeerJ, Others, Reviewer, 1
  29. 2016, Ethology Ecology and Evolution, Others, Reviewer, 1
  30. 2016, Genes & Genetic Systems, Others, Reviewer, 1
  31. 2017, Current Herpetology, Others, Reviewer
  32. 2017, Zoological Science, Others, Reviewer, 1
  33. 2017, Biological Journal of the Linnean Society, Others, Reviewer, 1
  34. 2017, Asian Herpetological Research, Others, Reviewer, 1
  35. 2018, Animal Biology, Others, Reviewer, 1
  36. 2018, Zoological Science, Others, Reviewer, 6
  37. 2018, Journal of Genetics, Others, Reviewer, 1
  38. 2018, Scientific Reports, Others, Reviewer, 2
  39. 2018, Molecular Biology Reports, Others, Reviewer, 2
  40. 2018, Herpetological Conservation and Biology, Others, Reviewer, 1