Hideki Shiba

Last Updated :2021/04/13

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Dentistry & Oral Health Sciences), Professor
E-mail
bashihihiroshima-u.ac.jp
Self-introduction
In January 2015, I succeeded the former Professor. At the same time, the name of the Department was changed to Biological Endodontics. The pursuit of this Department is to establish a new treatment for endodontic diseases by removal of bacteria and regulation of host cell function.

Basic Information

Major Professional Backgrounds

  • 1993/04/01, Hiroshima University, Dental Hospital, Clinical Fellow
  • 1993/11/01, Hiroshima University, Faculty of Dentistry, Research Associate
  • 2002/04/01, Hiroshima University, Graduate School of Biomedical Sciences, Research Associate
  • 2005/05/01, Hiroshima University Hospital, Lecturer
  • 2012/04/01, Hiroshima University, Institute of Biomedical & Health Sciences, Associate Professor
  • 2015/01/01, 2019/03/31, Hiroshima University, Institute of Biomedical & Health Sciences, Professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Dental Research, Japan, 1989/04, 1993/03
  • Hiroshima University, Faculty of Dentistry, Japan, 1983/04, 1989/03

Academic Degrees

  • Doctor of Philosophy in Dental Science, Hiroshima University

Educational Activity

  • 【Bachelor Degree Program】School of Dentistry : Program of Dentistry
  • 【Doctoral Program】Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Dental Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Dentistry;Conservative dentistry

Research Keywords

  • regulation of cell function
  • prevention
  • regeneration
  • Biological Endodontics

Educational Activity

Course in Charge

  1. 2021, Undergraduate Education, 4Term, Overview of Dentistry
  2. 2021, Undergraduate Education, 3Term, Examination and Diagnosis
  3. 2021, Undergraduate Education, Second Semester, Endodontics
  4. 2021, Undergraduate Education, Second Semester, Operative Dentistry
  5. 2021, Undergraduate Education, First Semester, Dental Research Practices
  6. 2021, Undergraduate Education, Second Semester, Dental Research Practices
  7. 2021, Undergraduate Education, 1Term, Quality and Safety Management in Dentistry
  8. 2021, Undergraduate Education, First Semester, General Seminar on Clinical Dentistry
  9. 2021, Undergraduate Education, First Semester, Dental Research Practices
  10. 2021, Undergraduate Education, Academic Year, Clinical Practice (Endodontics and Operative Dentistry)
  11. 2021, Undergraduate Education, Academic Year, Clinical Practice (Oral Clinical Examination)
  12. 2021, Undergraduate Education, 3Term, Endodontology
  13. 2021, Undergraduate Education, 1Term, Oral Health Management B
  14. 2021, Undergraduate Education, Second Semester, Clinical practice on oral health
  15. 2021, Undergraduate Education, Intensive, Clinical practice on oral health
  16. 2021, Undergraduate Education, 3Term, Endodontorogy
  17. 2021, Undergraduate Education, 1Term, Oral Health Management B
  18. 2021, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Biological Endodontics
  19. 2021, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Biological Endodontics
  20. 2021, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Biological Endodontics
  21. 2021, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Biological Endodontics

Research Activities

Academic Papers

  1. Irsogladine maleate regulates neutrophil migration and E-cadherin expression in gingival epithelium stimulated by Aggregatibacter actinomycetemcomitans, BIOCHEMICAL PHARMACOLOGY, 79(10), 1496-1505, 20100515
  2. The Antimicrobial Peptide LL37 Induces the Migration of Human Pulp Cells: A Possible Adjunct for Regenerative Endodontics, JOURNAL OF ENDODONTICS, 36(6), 1009-1013, 201006
  3. The expressions of claudin-1 and E-cadherin in junctional epithelium, JOURNAL OF PERIODONTAL RESEARCH, 45(4), 579-582, 201008
  4. Characteristics of High-Molecular-Weight Hyaluronic Acid as a Brain-Derived Neurotrophic Factor Scaffold in Periodontal Tissue Regeneration, TISSUE ENGINEERING PART A, 17(7-8), 955-967, 201104
  5. Effects of ageing on proliferative ability, and the expression of secreted protein, acidic and rich in cysteine(SPARC)and osteoprotegerin(osteoclastogenesis inhibitory factor)in cultures of human periodontal ligament cells, Mechanisms of Ageing and Development, 117, 69-77, 20000401
  6. Adherence of Bacteroides forsythus to host cells, Microbios, 101, 115-126, 19990401
  7. Cellular immuno-competence of infected root canal contents in pathogesesis of periapiacl lesion., J. Endodontics, 23, 213-216, 19970401
  8. Differential effects of various growth factos and cytokines on the syntheses of DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic and rich in cysteine (SPARC) , and alkaline phosphatase by human pulp cells in culture, J Cell Physiol, 174(2), 194-205, 19980201
  9. Transforming growth factor-b1 and basic fibroblast growth factor modulate osteocalcin and osteonectin/SPARC syntheses in vitamin D-activated pulp cells, J. Dent. Res., 80(7), 1653-1659, 20010701
  10. Expression of IL-1b and IL-8 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans, Cytokine, 14(3), 152-161, 20010501
  11. Effects of ageing on proliferative ability, and the expressions of secreted protein, acidic and rich in cysteine (SPARC) and osteoprotegerin (osteoclasogenesis inhibitory factor) in cultures of human periodontal ligament cells, Mech. Ageing Dev., 117(1-3), 69-77, 20000801
  12. Adherence of Bacteroides forsythus to host cells, Microbios, 101(399), 115-126, 20000401
  13. ★, Expression of osteoprotegerin (osteoclastogenesis inhibitory factor) in cultures of human dental mesenchymal cells and epithelial cells, J. Bone Miner. Res., 14(9), 1486-1492, 19990901
  14. ★, Differential effects of various growth factos and cytokines on the syntheses of DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic and rich in cysteine (SPARC) , and alkaline phosphatase by human pulp cells in culture, J Cell Physiol, 174(2), 194-205, 19980201
  15. Cellular immuno-competence of infected root canal contents in pathogenesis of periapical lesions, J. Endod., 23(4), 213-216, 19970401
  16. Effects of basic fibroblast growth factor on proliferation, the expression of osteonectin (SPARC) and alkaline phosphatase, and calcification in cultures of human pulp cells, Dev Biol, 170(2), 457-466, 19950801
  17. Transforming growth factor-beta-1 reduces alkaline phosphatase mRNA and activity and stimulates cell proliferation in cultures of human pulp cells, J. Dent. Res., 73(9), 1509-1514, 19940901
  18. Differential effects of various growth factors and cytokines on the syntheses of DNA, type I collagen, laminin, fibronectin, osteonectin/secreted protein, acidic and rich in cysteine (SPARC), and alkaline phosphatase by human pulp cells in culture., J. Cell. Phys., 174, 194-205., 19980401
  19. Actinobacillus actinomycetemcomitans CDT insuces cell cycle arrest in primary culture of human gingival fibroblasts. M.Ohara, Y.Ueno, H.Shiba, S.Nishikubo, M.Ikura, H.Kurihara, H.Komatsuzawa, M.Sugai. Dentistry in Japan. 40: 18-22. 2004., Dentistry in Japan, 40, 18-22, 20040401
  20. Outer membrane protein 100, a versatile virulence factor of Actinobacillus actinomycetemcomitans, Molecular Microbiology, 50(4), 1125-1139, 20030901
  21. Characteristics of periodontal ligament subpopulations obtained by sequential enzymatic digestion of rat molar periodontal ligament., BONE, 38, 420-426, 20060401
  22. Regulation of IL-8 by Irsogladine maleate is involved in abolishment of actinobacillus actinomycetemcomitans-induced reduction of gap-junctional intercellular communication., Cytokine, 34, 271-277, 20060401
  23. ★, Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge., Biochem. Biophys. Res. Commun., 333, 502-507, 20050401
  24. Differential effects of growth factors and cytokines on the synthesis of SPARC, DNA, fibronectin and alkaline phosphatase activity in human periodontal ligament cells., Cell. Biol. Int., 28, 281-286, 20040401
  25. The effect of extracellular calcium ion on gene expression of bone-related proteins in human pulp cells., J. Endodontics, 29, 104-107, 20030401
  26. ★, Brain-derived neurotrophic factor enhances periodontal tissue regeneration:, Tissue eng., 11(9-10), 1619-1630, 20051101
  27. Enhancement of periodontal tissue regeneration by transplantation of bone marrow mesenchymal stem cells, J. Periodontol, 75(9), 1281-1287, 20040401
  28. Expression of endothelins and their receptors in cells from human periodontal tissues, J. Periodontal. Res., 38(3), 269-275, 20030301
  29. Identification of marker genes distinguishing human periodontal ligament cells from human mesenchymal stem cells and human gingival fibroblasts., J Periodontal Res., 42(3), 283-6, 20070601
  30. Susceptibility of periodontopathogenic and cariogenic bacteria to defensins and potential therapeutic use of defensins in oral diseases, Curr Pham Des, 13(30), 3084-3095, 20070101
  31. Identification of marker genes distinguishing human periodontal ligament cells from human mesenchymal stem cells and human gingival fibroblasts,, J Periodontal Res, 42(3), 283-286, 20070601
  32. Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells, Cell Biol Int, 31(12), 1462-1469, 20071201
  33. Susceptibility of periodontopathogenic and cariogenic bacteria to defensins and potential therapeutic use of defensins in oral diseases., Curr Pharm Des., 13(30), 3084-3095, 20071001
  34. Gingival epithelial cells heterozygous for Toll-like receptor 4 polymorphisms Asp299Glp and Thr399Ile are hypo-responsive to Porphyromonas gingivalis, Genes Immun, 7(3), 190-200, 20060401
  35. The genetic basis of periodontitis, Periodontol 2000, 39, 91-117, 20050101
  36. Effect of bone morphogenetic proteins -4, -5 and -6 on DNA synthesis and expression of bone-related proteins in cultured human periodontal ligament cells, Cell Biol. Int., 28(10), 675-682, 20041001
  37. Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins., Cell Biol. Int., 29(2), 111-117, 20050201
  38. Porphyromonas gingivalis enhances FasL expression via up-regulation of NF?B-mediated gene transcription and induces apoptotic cell death in human gingival epithelial cells, Microbiology, 152(3), 797-806, 20060301
  39. Promotion of functioning of human periodontal ligament cells and human endothelial cells by nerve growth factor., J. Periodontol., 77(5), 800-807, 20060501
  40. Human autologous serum obtained using a completely closed bag system as a substitute for foetal calf serum in human mesenchymal stem cell cultures., Cell Biol. Int., 30(6), 521-524, 20060601
  41. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells., Infect. Immun., 74(9), 5211-5220, 20060901
  42. Identification of marker genes distinguishing human periodontal ligament cells from human mesenchymal stem cells and human gingival fibroblasts., J Periodontal. Res., 42(3), 283-286, 20070601
  43. An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells., Mycoses, 49(1), 14-17, 20060101
  44. Brain-derived neurotrophic factor enhances periodontal tissue regeneration, Tissue Engineering, 11(9-10), 1618-29, 20050901
  45. Effect of neurotrophins on differentiation, calcification and proliferation in cultures of human pulp cells, Cell Biol Int., 20070701
  46. Characterization of epithelial cells derived from periodontal ligament by gene expression patterns of bone-related and enamel proteins, Cell Biol Int., 29(2), 111-117, 20050201
  47. Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge., Biochem Biophys Res Commun, 333(2), 502-507, 20050729
  48. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans., Inflammation., 27(6), INFLAMMATION. 2003 DEC;27(6):341-9., 20031231
  49. Regulation of IL-8 by Irsogladine maleate is involved in abolishment of Actinobacillus actinomycetemcomitans-induced reduction of gap-junctional intercellular communication., Cytokine., 34(5-6), 271-7, 20060630
  50. Irsogladine maleate counters the IL-1b-induced suppression in gap-junctional intercellular communication without affecting increased ZO-1 levels by IL-1b in human gingival epithelial cells., Journal of Periodontal Research, 43(1), 96-102, 20080201
  51. ★, Brain-derived neurotrophic factor stimulates bone/cementum-related protein gene expression in cementoblasts, The Journal of Biological Chemistry, 283(23), 16259-16267, 20080601
  52. Irsogladine maleate abolishes the increase in interleukin-8 levels caused by outer membrane protein 29 from Aggregatibacter (Actinobacillus) actinomycetemcomitans through the ERK pathway in human gingival epithelial cells, Journal of Periodontal Research, 43(5), 508-513, 20081001
  53. Effect of Neurotrophin-4/5 on Bone/Cementum-Related Protein Expressions and DNA Synthesis in Cultures of Human Periodontal Ligament Cells, Journal of Periodontology, 79(11), 2182-2189, 20081101
  54. Humoral factors released from human periodontal ligament cells influence calcification and proliferation in human bone marrow mesenchymal stem cells, Journal of Periodontology, 79(12), 2361-2370, 20081201
  55. Neodymium-doped yttrium-aluminium-garnet laser irradiation abolishes the increase in interleukin-6 levels caused by peptidoglycan through the p38 mitogen-activated protein kinase pathway in human pulp cells, Journal of Endodontics, 35(3), 373-376, 20090301
  56. SPARC stimulates the synthesis of OPG/OCIF, MMP-2 and DNA in human periodontal ligament cells, JOURNAL OF ORAL PATHOLOGY & MEDICINE, 31(6), 345-352, 200207
  57. Differential effects of growth factors and cytokines on the synthesis of SPARC, DNA, fibronectin and alkaline phosphatase activity in human periodontal ligament cells, CELL BIOLOGY INTERNATIONAL, 28(4), 281-286, 2004
  58. Parotid secretory protein is expressed and inducible in human gingival keratinocytes, JOURNAL OF PERIODONTAL RESEARCH, 40(2), 153-157, 200504
  59. An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells, MYCOSES, 49(1), 14-17, 200601
  60. Gingival epithelial cells heterozygous for Toll-like receptor 4 polymorphisms Asp299Gly and Thr399lle are hypo-responsive to Porphyromonas gingivalis, GENES AND IMMUNITY, 7(3), 190-200, 200604
  61. Irsogladine maleate regulates epithelial barrier function in tumor necrosis factor-alpha-stimulated human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 47(1), 55-61, 201202
  62. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells, JOURNAL OF PERIODONTAL RESEARCH, 48(2), 228-234, 201304
  63. Irsogladine maleate regulates the inflammatory related genes in human gingival epithelial cells stimulated by Aggregatibacter actinomycetemcomitans, INTERNATIONAL IMMUNOPHARMACOLOGY, 15(2), 340-347, 201302
  64. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect, PEPTIDES, 46, 136-142, 201308
  65. BDNF mimetic compound LM22A-4 regulates cementoblast differentiation via the TrkB-ERK/Akt signaling cascade, INTERNATIONAL IMMUNOPHARMACOLOGY, 19(2), 245-252, 201404
  66. miR-584 Expressed in Human Gingival Epithelial Cells Is Induced by Porphyromonas gingivalis Stimulation and Regulates Interleukin-8 Production via Lactoferrin Receptor, JOURNAL OF PERIODONTOLOGY, 85(6), E198-E204, 201406
  67. Amphotericin B down-regulates Aggregatibacter actinomycetemcomitans-induced production of IL-8 and IL-6 in human gingival epithelial cells, CELLULAR IMMUNOLOGY, 290(2), 201-208, 201408
  68. Smad2 is Involved in Aggregatibacter actinomycetemcomitans-induced Apoptosis, JOURNAL OF DENTAL RESEARCH, 93(11), 1148-1154, 201411
  69. Introduction of a Mixture of beta-Tricalcium Phosphate Into a Complex of Bone Marrow Mesenchymal Stem Cells and Type I Collagen Can Augment the Volume of Alveolar Bone Without Impairing Cementum Regeneration, JOURNAL OF PERIODONTOLOGY, 86(3), 456-464, 201503
  70. Houttuynia cordata suppresses the Aggregatibacter actinomycetemcomitans-induced increase of inflammatory-related genes in cultured human gingival epithelial cells, JOURNAL OF DENTAL SCIENCES, 10(1), 88-94, 201503
  71. The differential expression of mgl mRNA by Porphyromonas gingivalis affects the production of methyl mercaptan, ORAL DISEASES, 21(5), 626-633, 201507
  72. ★, Clumps of a mesenchymal stromal cell/extracellular matrix complex can be a novel tissue engineering therapy for bone regeneration, CYTOTHERAPY, 17(7), 860-873, 201507
  73. Brain-derived neurotrophic factor prevents the endothelial barrier dysfunction induced by interleukin-1 beta and tumor necrosis factor-alpha, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 444-451, 201508
  74. Interleukin-8 induces DNA synthesis, migration and down-regulation of cleaved caspase-3 in cultured human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 479-485, 201508
  75. Irsogladine maleate inhibits Porphyromonas gingivalis-mediated expression of toll-like receptor 2 and interleukin-8 in human gingival epithelial cells, JOURNAL OF PERIODONTAL RESEARCH, 50(4), 486-493, 201508
  76. LL37 induces VEGF expression in dental pulp cells through ERK signalling, INTERNATIONAL ENDODONTIC JOURNAL, 48(7), 673-679, 201507
  77. Involvement of smad2 and Erk/Akt cascade in TGF-beta 1-induced apoptosis in human gingival epithelial cells, CYTOKINE, 75(1), 165-173, 201509
  78. Relationship between length variations in Ser/Asp-rich repeats in phosphophoryn and in vitro precipitation of calcium phosphate, ARCHIVES OF ORAL BIOLOGY, 60(9), 1263-1272, 201509
  79. Sequential process in brain-derived neurotrophic factor-induced functional periodontal tissue regeneration, EUROPEAN JOURNAL OF ORAL SCIENCES, 124(2), 141-150, 201604
  80. Distinction Between Cell Proliferation and Apoptosis Signals Regulated by Brain-Derived Neurotrophic Factor in Human Periodontal Ligament Cells and Gingival Epithelial Cells, JOURNAL OF CELLULAR BIOCHEMISTRY, 117(7), 1543-1555, 201607
  81. Azithromycin recovers reductions in barrier function in human gingival epithelial cells stimulated with tumor necrosis factor-alpha, ARCHIVES OF ORAL BIOLOGY, 62, 64-69, 201602
  82. Aggregatibacter actinomycetemcomitans outer membrane protein 29 induces TGF-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/FAK cascade, Cell Microbiol, 18(18), 1723-1738, 2016
  83. Dentin sialophosphoproteinisapotentiallylatentbioactiveprotein in dentin, Journal of Oral Biosciences, 58(58), 134-142, 2016
  84. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF--regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrin1/FAK cascade, CELLULAR MICROBIOLOGY, 18(12), 1723-1738, 201612
  85. Dentin sialophosphoprotein is a potentially latent bioactive protein in dentin, Journal of Oral Biosciences, 58(4), 134-142, 201611
  86. A novel gingival overgrowth mouse model induced by the combination of CsA and ligature-induced inflammation, JOURNAL OF IMMUNOLOGICAL METHODS, 445, 31-36, 201706
  87. Useful immunochromatographic assay of calprotectin in gingival crevicular fluid for diagnosis of diseased sites in patients with periodontal diseases, JOURNAL OF PERIODONTOLOGY, 89(1), 67-75, 2018
  88. Genome-wide identification of chromatin-enriched RNA reveals that unspliced dentin matrix protein-1 mRNA regulates cell proliferation in squamous cell carcinoma, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 495(3), 2303-2309, 20180115
  89. Combined endodontic and periodontal management of a class 3 invasive cervical resorption in a mandibular first molar, CLINICAL CASE REPORTS, 6(10), 2005-2010, 201810
  90. Glycyrrhizic acid suppresses inflammation and reduces the increased glucose levels induced by the combination of Porphyromonas gulae and ligature placement in diabetic model mice, INTERNATIONAL IMMUNOPHARMACOLOGY, 68, 30-38, 201903
  91. Dental pulp cell-derived powerful inducer of TNF-alpha comprises PKR containing stress granule rich microvesicles, SCIENTIFIC REPORTS, 9, 20190307
  92. Interleukin (IL)-35 Suppresses IL-6 and IL-8 Production in IL-17A-Stimulated Human Periodontal Ligament Cells, INFLAMMATION, 42(3), 835-840, 201906
  93. Heparin-LL37 complexes are less cytotoxic for human dental pulp cells and have undiminished antimicrobial and LPS-neutralizing abilities, INTERNATIONAL ENDODONTIC JOURNAL, 52(9), 1327-1343, 201909
  94. Dentin phosphoprotein inhibits lipopolysaccharide-induced macrophage activation independent of its serine/aspartic acid-rich repeats, ARCHIVES OF ORAL BIOLOGY, 110, 202002
  95. Optineurin regulates osteoblastogenesis through STAT1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 525(4), 889-894, 20200514
  96. A small nuclear acidic protein (MTI-II, Zn2+-binding protein, parathymosin) attenuates TNF-alpha inhibition of BMP-induced osteogenesis by enhancing accessibility of the Smad4-NF-kappa B p65 complex to Smad binding element, MOLECULAR AND CELLULAR BIOCHEMISTRY, 469(1-2), 133-142, 202006
  97. A mandibular second molar with a middle mesial root canal, CLINICAL CASE REPORTS, 8(6), 1015-1020, 202006
  98. Oral environment and taste function of Japanese HIV-infected patients treated with antiretroviral therapy, AIDS CARE-PSYCHOLOGICAL AND SOCIO-MEDICAL ASPECTS OF AIDS/HIV, 32(7), 829-834, 20200702