Masayuki Shimada

Last Updated :2023/09/06

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
E-mail
mashimadhiroshima-u.ac.jp
Self-introduction
I'm interested in reproductive biology, especially, ovarian functions, spermatogenesis, sperm motility, fertilization and also focusing on developing reproductive technology, such as artificial insemination, in vitro fertilization and sperm sexing.

Basic Information

Academic Degrees

  • Doctor of Philosophy, Yamaguchi University
  • Master of Agriculture, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Applied Biological Science : Department of Applied Biological Science : Molecular Agro-Life Science Program
  • [Master's Program] Graduate School of Innovation and Practice for Smart Society :
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science
  • [Doctoral Program] Graduate School of Innovation and Practice for Smart Society :
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Food and AgriLife Science
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science

Research Fields

  • Agricultural sciences;Animal life science;Animal production science

Research Keywords

  • oocyte|ovulation
  • fertilization/IVF
  • in vitro maturation
  • ovary
  • assisted reproductive technology
  • spermatogenesis
  • testis
  • semen storage

Educational Activity

Course in Charge

  1. 2023, Liberal Arts Education Program1, 3Term, Cell Science
  2. 2023, Liberal Arts Education Program1, Intensive, Food Culture
  3. 2023, Liberal Arts Education Program1, First Semester, Food culture
  4. 2023, Undergraduate Education, Intensive, Laboratory Work in General Biology II
  5. 2023, Undergraduate Education, 1Term, Introduction to Physiology
  6. 2023, Undergraduate Education, 4Term, Research Front of Food and AgriLife Science
  7. 2023, Undergraduate Education, 1Term, Reproductive Biology
  8. 2023, Undergraduate Education, Second Semester, Graduation Thesis I
  9. 2023, Undergraduate Education, First Semester, Graduation Thesis II
  10. 2023, Undergraduate Education, Second Semester, Graduation Thesis III
  11. 2023, Undergraduate Education, Intensive, Problem Based Learning for Molecular Agro-Life ScienceI
  12. 2023, Undergraduate Education, 4Term, Molecular Cell Biology
  13. 2023, Undergraduate Education, 4Term, Bio-Analytical Science
  14. 2023, Undergraduate Education, 3Term, Reading of Foreign Literature in Molecular Agro-Life Science
  15. 2023, Graduate Education (Master's Program) , 2Term, societal implementation of life science
  16. 2023, Graduate Education (Master's Program) , 1Term, Exercises in Food andAgriLife Science A
  17. 2023, Graduate Education (Master's Program) , 2Term, Exercises in Food andAgriLife Science A
  18. 2023, Graduate Education (Master's Program) , 3Term, Exercises in Food andAgriLife Science B
  19. 2023, Graduate Education (Master's Program) , 4Term, Exercises in Food andAgriLife Science B
  20. 2023, Graduate Education (Master's Program) , 2Term, Assisted Reproductive Technology for Animal Production
  21. 2023, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  22. 2023, Graduate Education (Master's Program) , 1Term, Exercises in Bioresource Science A
  23. 2023, Graduate Education (Master's Program) , 2Term, Exercises in Bioresource Science A
  24. 2023, Graduate Education (Master's Program) , 3Term, Exercises in Bioresource ScienceB
  25. 2023, Graduate Education (Master's Program) , 4Term, Exercises in Bioresource ScienceB
  26. 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Bioresource Science
  27. 2023, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  28. 2023, Graduate Education (Master's Program) , 4Term, Introduction to Genetics and Genomics

Research Activities

Academic Papers

  1. Female reproductive life span is extended by targeted removal of fibrotic collagen from the mouse ovary, SCIENCE ADVANCES, 8(24), 20220617
    Link to Paper Search Results by DOI
  2. Toll-like Receptor 2 is Involved in Calcium Influx and Acrosome Reaction to Facilitate Sperm Penetration to Oocytes During in vitro Fertilization in Cattle, FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 10, 20220224
    Link to Paper Search Results by DOI
  3. Sperm interaction with the uterine innate immune system: toll-like receptor 2 (TLR2) is a main sensor in cattle, REPRODUCTION FERTILITY AND DEVELOPMENT, 34(2), 139-148, 2022
    Link to Paper Search Results by DOI
  4. Adverse effect of superoxide-induced mitochondrial damage in granulosa cells on follicular development in mouse ovaries, FREE RADICAL BIOLOGY AND MEDICINE, 163, 344-355, 20210201
    Link to Paper Search Results by DOI
  5. Peptidoglycan Switches Off the TLR2-Mediated Sperm Recognition and Triggers Sperm Localization in the Bovine Endometrium, FRONTIERS IN IMMUNOLOGY, 11, 20210211
    Link to Paper Search Results by DOI
  6. Neutrophils recognize and amplify IFNT signals derived from day 7 bovine embryo for stimulation of ISGs expression in vitro: A possible implication for the early maternal recognition of pregnancy, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 553, 37-43, 20210514
    Link to Paper Search Results by DOI
  7. Saturated fatty acids accelerate linear motility through mitochondrial ATP production in bull sperm, REPRODUCTIVE MEDICINE AND BIOLOGY, 20(3), 289-298, 202107
    Link to Paper Search Results by DOI
  8. Day 7 Embryos Change the Proteomics and Exosomal Micro-RNAs Content of Bovine Uterine Fluid: Involvement of Innate Immune Functions, FRONTIERS IN GENETICS, 12, 20210628
    Link to Paper Search Results by DOI
  9. Cortisol induces follicle regression, while FSH prevents cortisol-induced follicle regression in pigs, MOLECULAR HUMAN REPRODUCTION, 27(7), 202107
    Link to Paper Search Results by DOI
  10. LH Induces De Novo Cholesterol Biosynthesis via SREBP Activation in Granulosa Cells During Ovulation in Female Mice, ENDOCRINOLOGY, 162(11), 202111
    Link to Paper Search Results by DOI
  11. Large-scale DNA demethylation occurs in proliferating ovarian granulosa cells during mouse follicular development, COMMUNICATIONS BIOLOGY, 4(1), 20211125
    Link to Paper Search Results by DOI
  12. Sperm enter glands of preovulatory bovine endometrial explants and initiate inflammation, REPRODUCTION, 159(2), 181-192, 202002
    Link to Paper Search Results by DOI
  13. Roadmap to pregnancy in the first 7 days post-insemination in the cow: Immune crosstalk in the corpus luteum, oviduct, and uterus, THERIOGENOLOGY, 150, 313-320, 20200701
    Link to Paper Search Results by DOI
  14. A simple sperm-sexing method that activates TLR7/8 on X sperm for the efficient production of sexed mouse or cattle embryos, NATURE PROTOCOLS, 15(8), 2645-2667, 202008
    Link to Paper Search Results by DOI
  15. Pretreatment of ovaries with collagenase before vitrification keeps the ovarian reserve by maintaining cell-cell adhesion integrity in ovarian follicles, SCIENTIFIC REPORTS, 10(1), 20200422
    Link to Paper Search Results by DOI
  16. Itaconate regulates the glycolysis/pentose phosphate pathway transition to maintain boar sperm linear motility by regulating redox homeostasis, FREE RADICAL BIOLOGY AND MEDICINE, 159, 44-53, 20201101
    Link to Paper Search Results by DOI
  17. Cutting the ovarian surface improves the responsiveness to exogenous hormonal treatment in aged mice, REPRODUCTIVE MEDICINE AND BIOLOGY, 19(4), 415-424, 202010
    Link to Paper Search Results by DOI
  18. Peptidoglycan disrupts early embryo-maternal crosstalk via suppression of ISGs expression induced by interferon-tau in the bovine endometrium, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 532(1), 101-107, 20201029
    Link to Paper Search Results by DOI
  19. Sensing sperm via maternal immune system: a potential mechanism for controlling microenvironment for fertility in the cow, JOURNAL OF ANIMAL SCIENCE, 98, S88-S95, 202008
    Link to Paper Search Results by DOI
  20. Methyl-beta cyclodextrin and creatine work synergistically under hypoxic conditions to improve the fertilization ability of boar ejaculated sperm, ANIMAL SCIENCE JOURNAL, 91(1), 2020
    Link to Paper Search Results by DOI
  21. Impact of lipopolysaccharide administration on luteinizing hormone/choriogonadotropin receptor (Lhcgr) expression in mouse ovaries, JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 142, 202011
    Link to Paper Search Results by DOI
  22. Iron deficiency induces female infertile in order to failure of follicular development in mice, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 66(5), 475-483, 202010
  23. Mitochondrial Protein Turnover Is Critical for Granulosa Cell Proliferation and Differentiation in Antral Follicles, JOURNAL OF THE ENDOCRINE SOCIETY, 3(2), 324-339, 201902
    Link to Paper Search Results by DOI
  24. Gene Expression and Protein Synthesis in Mitochondria Enhance the Duration of High-Speed Linear Motility in Boar Sperm, FRONTIERS IN PHYSIOLOGY, 10, 20190312
    Link to Paper Search Results by DOI
  25. TLR2/4 signaling pathway mediates sperm-induced inflammation in bovine endometrial epithelial cells in vitro, PLOS ONE, 14(4), 20190417
    Link to Paper Search Results by DOI
  26. Cyclooxygenase-2 is acutely induced by CCAAT/enhancer-binding protein beta to produce prostaglandin E-2 and F-2 alpha following gonadotropin stimulation in Leydig cells, MOLECULAR REPRODUCTION AND DEVELOPMENT, 86(7), 786-797, 201907
    Link to Paper Search Results by DOI
  27. Activation of Toll-like receptor 7/8 encoded by the X chromosome alters sperm motility and provides a novel simple technology for sexing sperm, PLOS BIOLOGY, 17(8), 201908
    Link to Paper Search Results by DOI
  28. Negative effects of ROS generated during linear sperm motility on gene expression and ATP generation in boar sperm mitochondria, FREE RADICAL BIOLOGY AND MEDICINE, 141, 159-171, 201909
    Link to Paper Search Results by DOI
  29. Induction of immune-related gene expression by seminal exosomes in the porcine endometrium, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 495(1), 1094-1101, 20180101
    Link to Paper Search Results by DOI
  30. Inductions of granulosa cell luteinization and cumulus expansion are dependent on the fibronectin-integrin pathway during ovulation process in mice, PLOS ONE, 13(2), 20180208
    Link to Paper Search Results by DOI
  31. Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass, GENES TO CELLS, 23(3), 136-145, 201803
    Link to Paper Search Results by DOI
  32. A proinflammatory response of bovine endometrial epithelial cells to active sperm in vitro, MOLECULAR REPRODUCTION AND DEVELOPMENT, 85(3), 215-226, 201803
    Link to Paper Search Results by DOI
  33. Oviduct epithelium induces interferon-tau in bovine Day-4 embryos, which generates an anti-inflammatory response in immune cells, SCIENTIFIC REPORTS, 8, 20180518
    Link to Paper Search Results by DOI
  34. Evidence that interferon-tau secreted from Day-7 embryo in vivo generates anti-inflammatory immune response in the bovine uterus, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 500(4), 879-884, 20180612
    Link to Paper Search Results by DOI
  35. The Cell Type-Specific Expression of Lhcgr in Mouse Ovarian Cells: Evidence for a DNA-Demethylation-Dependent Mechanism, ENDOCRINOLOGY, 159(5), 2062-2074, 201805
    Link to Paper Search Results by DOI
  36. Creatine enhances the duration of sperm capacitation: a novel factor for improving in vitro fertilization with small numbers of sperm, HUMAN REPRODUCTION, 33(6), 1117-1129, 201806
    Link to Paper Search Results by DOI
  37. Bovine embryo induces an anti-inflammatory response in uterine epithelial cells and immune cells in vitro: possible involvement of interferon tau as an intermediator, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 63(4), 425-434, 2017
  38. The acceleration of reproductive aging in Nrg1(flox/flox);Cyp19-Cre female mice, AGING CELL, 16(6), 1288-1299, 201712
    Link to Paper Search Results by DOI
  39. De Novo-Synthesized Retinoic Acid in Ovarian Antral Follicles Enhances FSH-Mediated Ovarian Follicular Cell Differentiation and Female Fertility, ENDOCRINOLOGY, 157(5), 2160-2172, 2016
    Link to Paper Search Results by DOI
  40. The Novel Pig In Vitro Maturation System to Improve Developmental Competence of Oocytes Derived from Atretic Nonvascularized Follicles, BIOLOGY OF REPRODUCTION, 95(4), 20161001
    Link to Paper Search Results by DOI
  41. Neuregulin 1 Regulates Proliferation of Leydig Cells to Support Spermatogenesis and Sexual Behavior in Adult Mice, ENDOCRINOLOGY, 157(12), 4899-4913, 2016
    Link to Paper Search Results by DOI
  42. Protein Kinase C (PKC) Increases TACE/ADAM17 Enzyme Activity in Porcine Ovarian Somatic Cells, Which Is Essential for Granulosa Cell Luteinization and Oocyte Maturation, ENDOCRINOLOGY, 155(3), 1080-1090, 2014
    Link to Paper Search Results by DOI
  43. The lncRNA Neat1 is required for corpus luteum formation and the establishment of pregnancy in a subpopulation of mice, DEVELOPMENT, 141(23), 4618-4627, 2014
    Link to Paper Search Results by DOI
  44. Targeted Disruption of Nrg1 in Granulosa Cells Alters the Temporal Progression of Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 28(5), 706-721, 2014
  45. Anti-Bacterial Factors Secreted From Cumulus Cells of Ovulated COCs Enhance Sperm Capacitation During In Vitro Fertilization, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 69(2), 168-179, 2013
    Link to Paper Search Results by DOI
  46. Androgen/androgen receptor pathway regulates expression of the genes for cyclooxygenase-2 and amphiregulin in periovulatory granulosa cells, MOLECULAR AND CELLULAR ENDOCRINOLOGY, 369(42737), 42-51, 20130430
    Link to Paper Search Results by DOI
  47. New strategies of boar sperm cryopreservation: Development of novel freezing and thawing methods with a focus on the roles of seminal plasma, ANIMAL SCIENCE JOURNAL, 83(9), 623-629, 2012
    Link to Paper Search Results by DOI
  48. Artificial Insemination With Seminal Plasma Improves the Reproductive Performance of Frozen-Thawed Boar Epididymal Spermatozoa, JOURNAL OF ANDROLOGY, 33(5), 990-998, 2012
    Link to Paper Search Results by DOI
  49. Adiponectin and its receptors modulate granulosa cell and cumulus cell functions, fertility, and early embryo development in the mouse and human, FERTILITY AND STERILITY, 98(2), 471-+, 2012
    Link to Paper Search Results by DOI
  50. The Release of EGF Domain from EGF-like Factors by a Specific Cleavage Enzyme Activates the EGFR-MAPK3/1 Pathway in Both Granulosa Cells and Cumulus Cells During the Ovulation Process, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 58(5), 510-514, 2012
  51. Endogenous acetaldehyde toxicity during antral follicular development in the mouse ovary, REPRODUCTIVE TOXICOLOGY, 33(3), 322-330, 2012
  52. EGF-Like Factors Induce Expansion of the Cumulus Cell-Oocyte Complexes by Activating Calpain-Mediated Cell Movement, ENDOCRINOLOGY, 153(8), 3949-3959, 2012
  53. The addition of calcium ion chelator, EGTA to thawing solution improves fertilizing ability in frozen-thawed boar sperm, ANIMAL SCIENCE JOURNAL, 82(3), 412-419, 2011
    Link to Paper Search Results by DOI
  54. Toll-like receptors (TLR) 2 and 4 on human sperm recognize bacterial endotoxins and mediate apoptosis, HUMAN REPRODUCTION, 26(10), 2799-2806, 2011
    Link to Paper Search Results by DOI
  55. Positive Feedback Loop Between Prostaglandin E2 and EGF-Like Factors Is Essential for Sustainable Activation of MAPK3/1 in Cumulus Cells During In Vitro Maturation of Porcine Cumulus Oocyte Complexes, BIOLOGY OF REPRODUCTION, 85(5), 1073-1082, 2011
    Link to Paper Search Results by DOI
  56. LH-Induced Neuregulin 1 (NRG1) Type III Transcripts Control Granulosa Cell Differentiation and Oocyte Maturation, MOLECULAR ENDOCRINOLOGY, 25(1), 104-116, 2011
  57. Possible involvement of phosphatidylinositol 3-kinase in the maintenance of metaphase II attest in porcine oocytes matured in vitro, ANIMAL SCIENCE JOURNAL, 81(1), 42-47, 2010
    Link to Paper Search Results by DOI
  58. beta-Catenin (CTNNB1) Promotes Preovulatory Follicular Development but Represses LH-Mediated Ovulation and Luteinization, MOLECULAR ENDOCRINOLOGY, 24(8), 1529-1542, 2010
    Link to Paper Search Results by DOI
  59. Polymyxin B neutralizes bacteria-released endotoxin and improves the quality of boar sperm during liquid storage and cryopreservation, THERIOGENOLOGY, 74(9), 1691-1700, 2010
    Link to Paper Search Results by DOI
  60. Progesterone is Essential for Maintenance of Tace/Adam17 mRNA Expression, But not EGF-like Factor, in Cumulus Cells, Which Enhances the EGF Receptor Signaling Pathway During In Vitro Maturation of Porcine COCs, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 56(3), 315-323, 2010
  61. Activation of PKA, p38 MAPK and ERK1/2 by gonadotropins in cumulus cells is critical for induction of EGF-like factor and TACE/ADAM17 gene expression during in vitro maturation of porcine COCs, JOURNAL OF OVARIAN RESEARCH, 2(1), 2009
    Link to Paper Search Results by DOI
  62. MAPK3/1 (ERK1/2) in Ovarian Granulosa Cells Are Essential for Female Fertility, SCIENCE, 324(5929), 938-941, 20090515
    Link to Paper Search Results by DOI
  63. Interleukin-6: An Autocrine Regulator of the Mouse Cumulus Cell-Oocyte Complex Expansion Process, ENDOCRINOLOGY, 150(7), 3360-3368, 2009
    Link to Paper Search Results by DOI
  64. Selective expression of Kras(G12D) in granulosa cells of the mouse ovary causes defects in follicle development and ovulation, DEVELOPMENT, 135(12), 2127-2137, 20080615
    Link to Paper Search Results by DOI
  65. Hyaluronan fragments generated by sperm-secreted hyaluronidase stimulate cytokine/chemokine production via the TLR2 and TLR4 pathway in cumulus cells of ovulated COCs, which may enhance fertilization, DEVELOPMENT, 135(11), 2001-2011, 20080601
    Link to Paper Search Results by DOI
  66. Sequential exposure of porcine cumulus cells to FSH and/or LH is critical for appropriate expression of steroidogenic and ovulation-related genes that impact oocyte maturation in vivo and in vitro, REPRODUCTION, 136(1), 9-21, 2008
    Link to Paper Search Results by DOI
  67. Immune-like mechanisms in ovulation, TRENDS IN ENDOCRINOLOGY AND METABOLISM, 19(6), 191-196, 2008
    Link to Paper Search Results by DOI
  68. The involvement of the Toll-like receptor family in ovulation, JOURNAL OF ASSISTED REPRODUCTION AND GENETICS, 25(6), 223-228, 2008
  69. Assessment of human oocyte quality by cumulus cell morphology and circulating hormone profile, Reproductive BioMedicine Online, 14(1), 20070101
  70. Involvment of Ca2+-dependent proteasome in the degradation of both cyclin B1 and Mos during spontaneous activation of matured rat oocytes., Theriogenology, 67(3), 475-485, 20070301
  71. Synaptosomal-associated protein 25 gene expression is hormonally regulated during ovulation and is involved in cytokine/chemokine exocytosis from granulosa cells, MOLECULAR ENDOCRINOLOGY, 21(10), 2487-2502, 2007
    Link to Paper Search Results by DOI
  72. Hormone-induced expression of tumor necrosis factor alpha-converting enzyme/a disintegrin and metalloprotease-17 impacts porcine cumulus cell oocyte complex expansion and meiotic maturation via ligand activation of the epidermal growth factor receptor, ENDOCRINOLOGY, 148(12), 6164-6175, 2007
    Link to Paper Search Results by DOI
  73. Gene expression profiles of cumulus cell oocyte complexes during ovulation reveal that cumulus cells exhibit a diverse array of neuronal and immune-like functional activities: Are these cells multi-potential?, Molecular Endocrinology, 20(6), in press, 20060601
  74. Paracrine and autocrine regulation of EGF-like factors in cumulus oocyte complexes (COCs) and granulosa cells: key roles for prostanglandin synthase 2 (Ptgs2) and progesterone receptor (Pgr), Molecular Endocrinology, 20(6), in press, 20060601
  75. Induced expression of pattern recognition receptors in cumulus oocyte complexes: Novel evidence for innate immune-like functions during ovulation, MOLECULAR ENDOCRINOLOGY, 20(12), 3228-3239, 2006
    Link to Paper Search Results by DOI
  76. Gonadotropin-induced delta 14-reductase and delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, Endocrinology, 146, 186-194, 20050101
  77. The timing of MAP kinase inactivation effects on emission of polar body in porcine oocytes activated by Ca2+ ionophore, Molecular Reproduction and Development, 70, 64-69, 20050201
  78. Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle., Animal Reproduction Science, 87, 1-10, 20050601
  79. Contribution of high p34cdc2 kinase activity to premature chromosome condensation of injected somatic cell nuclei in rat oocytes., Reproduction, 129, 172-180, 20050401
  80. Transcripts encoding the enzymes that convert acetyl-CoA to cholesterol are induced in cumulus cells and are essential for progesterone biosynthesis and meiotic resumption of porcine oocytes., Japanese Journal of Reproductive Endocrinology, 10, 15-20, 20051101
  81. TAF4b, a TBP Associated Factor is Required for Multiple Aspects of Oocyte Development., Developmental Biology, 208(6), 405-409, 20050615
  82. Mice null for Frizzled4 (Fzd4-/-) are infertile and exhibit impaired corpus lutea formation and function: a critical for vascularization., Biology of Reproduction, 73(7), 1135-1146, 20050729
  83. Mitogen-Activated Protein Kinase Kinase Inhibitor Suppresses Cyclin B1 Synthesis and Reactivation of p34cdc2 Kinase, Which Improves Pronuclear Formation Rate in Matured Porcine Oocytes Activated by Ca2+ Ionophore, Biology of Reproduction, 70, 797-804, 20040301
  84. Dramatic changese in motility and penetration competence of miniture pig spermatozoa frozen after exposure to seminal plasma, Theriogenology, 61(42769), 351-364, 20040101
  85. Expression of two progesterone receptor isoforms in cumulus cells and their roles during meiotic resumption of porcine oocytes, Journal of Molecular Endocrinology, in press, 20040401
  86. The role of Calcium/Calmodulin-dependent protein kinase II (CaMKII) on inactivation of MAP kinase and p34cdc2 kinase during fertilization and activation in pig oocytes, Reproduction, 128, 409-415, 20040401
  87. Down-regulated expression of ADAMTS-1 by progesterone receptor antagonist is associated with impaired expansion of porcine cumulus-oocyte complexes, Endocrinology, 145, 4603-4614, 20040401
  88. Effects of adding LH to FSH-containing medium on progesterone-induced differentiation of cumulus cells during meiotic resumption of porcine oocytes, Animal Science Journal, in press, 20040401
  89. FSH-induced activation of PI 3-kinase-PKB pathway is essential for LH receptor formation in cumulus cells during meiotic resumption of porcine oocytes., Japanese Journal of Reproductive Endocrinology, in press, 20040401
  90. Motility and penetration competence of frozen-thawed miniature pig spermatozoa are substantially altered by exposure to seminal plasma before freezing, THERIOGENOLOGY, 61(42769), 351-364, 20040115
    Link to Paper Search Results by DOI
  91. The production of progesterone from de novo-synthesized cholesterol in cumulus cells, and its physiological role during meiotic resumption of porcine oocytes, Biology of Reproduction, 68(4), 1193-1198, 20030401
  92. Effects of estrone sulfate injection on reproductive functions in male Japanese quail., Journal of Poultry Science, 40, 243-247, 20031001
  93. PI 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes., Journal of Endocrinology, 179, 25-34, 20031001
  94. Effect of Protein kinase C activator on mitogen-activated protein kinase and p34cdc2 kinase activity during parthenogenetic activation of porcine oocytes by calcium ionophore., Biology of Reproduction, 69, 1675-1682, 20031101
  95. LH receptor formation in cumulus cells surrounding porcine oocytes, and its role during meiotic maturation of porcine oocytes., Biology of Reproduction, 68(4), 1142-1149, 20030401
  96. Time dependent changes in progesterone receptor expression in cumulus cells during meiotic resumption of porcine oocytes, Journal of Mammalian Ova Research, 20, 113-117, 20031001
  97. LH reduces proliferative activity of cumulus cells and accelerates GVBD of porcine oocytes, Molecular and Cellular Endocrinology, 209, 43-50, 20030401
  98. Roles of cAMP in Regulation of both MAP kinase and p34cdc2 kinase activity during Meiotic Progression,especially beyond the MI stage., Molecular Reproduction and Development, 62(1), 124-131, 20020501
  99. ★, FSH and LH induce progesterone production and progesterone receptor synthesis in cumulus cells: a requirement for meiotic resumption in porcine oocytes., Molecular Human Reproduction, 8, 621-628, 20020701
  100. Delay of nuclear maturation and reduction in developmental competence of pig oocytes after mineral oil overlay of in vitro maturation media., Reproduction, 124, 557-564, 20021001
  101. Both Ca2+-PKC pathway and cAMP-PKA pathway require for progesterone production in FSH- and LH-stimulated cumulus cells during in vitro maturation of porcine oocytes., J Mamm Ova Res, 19(3), 81-88, 20021001
  102. Progression of nuclear maturation and p34cdc2 kinase activity in porcine oocytes during in vitro culture in different medium, J Mamm Ova Res, 18(1), 39-43, 20010401
  103. Survival of Boar Spermatozoa Frozen in Diluents of Varying Osmolality., Theriogenology, 56(8), 447-458, 20010401
  104. ★, Inhibition of PI 3-kinase or MEK Leads to Suppression of p34cdc2 kinase Activity and Meiotic Progression beyond the MI stage in Porcine Oocyte Surrounded with Cumulus Cells., Biology of Reproduction, 65(2), 442-448, 20010401
  105. Dynamic changes of connexin-43, gap junctional protein, in outer layers of cumulus cells are regulated by PKC and PI 3-kinase during meiotic resumption in porcine oocytes, Biology of Reproduction, 64(4), 1255-1263, 20010401
  106. PI 3-kinase in cumulus cells and oocytes is responsible for activation of oocyte MAP kinase during meiotic progression beyond the MI stage in pigs, Biology of Reproduction, 64(4), 1106-1114, 20010401
  107. The drop of cAMP level due to the closure of gap junctional communication between cumulus cells and oocyte is essential for meiotic progression beyond the MI stage in porcine oocytes, J Mamm Ova Res, 18(3), 99-105, 20010401
  108. Wortmannin, a specific phosphatidylinositol 3-kinase inhibitor, blocks in vitro fertilization and embryonic development of bovine oocytes matured in vitro with epidermal growth factor., J Mamm Ova Res, 17(3), 109-114, 20000401
  109. Meiotic Maturation of Bovine Oocytes Cultured In vitro with EGF and Wortmannin., J Mamm Ova Res, 17(3), 103-108, 20000401
  110. Activation by stimuli of sperm penetration in bovine oocytes treated with high concentration of BAPTA/AM., J Mamm Ova Res, 17(3), 96-102, 20000401
  111. Effects of wortmannin on the kinetics of GVBD and the activities of the maturation-promoting factor and mitogen-activated protein kinase during bovine oocyte maturation in vitro, Theriogenology, 53(5), 1797-1806, 20000401
  112. Phosphorylation of Connexin-43, Gap Junctional Protein, in Cumulus Cells Are Regulated by Mitogen Activated Protein Kinase and Phosphatidylinositol 3-Kinase during In Vitro Meiotic Resumption in Porcine Follicular Oocytes., J Mamm Ova Res, 16(3), 37-42, 19990401
  113. A Role for PI 3-Kinase in bovine oocytes nuclear maturation., Theriogenology, 50(7), 347-356, 19980401
  114. ★, Effects of Phosphatidylinositol 3-Kinase Inhibitors, Wortmannin and LY294002 on Germinal Vesicle Breakdown (GVBD) in Porcine Oocytes., J Reprod Dev, 44(5), 281-288, 19980401

Invited Lecture, Oral Presentation, Poster Presentation

  1. The dramatic changes cumulus cell functions during ovulation process impact meiotic progression and fertilization ability of oocyte., Masayuki Shimada, 14th International symposium of Immunology of Reproduction, 2015, With Invitation, English, Verna, Bulgaria,
  2. The aging ovary: restriction of follicle growth by abnormal endocrine functions and ovarian stromal matrix fibrosis, Masayuki Shimada, 15th International Symposium of Immunology of Reproduction, 2018/06/14, With Invitation, English
  3. The Aging Ovary: Abnormal Endocrine Functions Is there a role for Androgens, AR and Theca Cell Dysfunction?, JoAnne S. Richards, Masayuki Shimada, Cellular and Molecular Aging of the Reproductive System, 2018/09/10, With Invitation, English

Patented

  1. Patent, JP4783883, 2011/07/22
  2. Patent, JP5422848, 2013/12/06
  3. Patent, KR:10-1457143, 2014/10/27
  4. ZL201080026618.5, 2015/01/07
  5. 2765855, 2015/02/03
  6. 5733829, 2015/04/24
  7. 5904369, 2016/03/25
  8. Patent, 5920787, 2016//4//2
  9. Patent, 2011327417, 2016//9//1
  10. Patent, 9439414, 2016//9//1
  11. Patent, 2639298, 2017//1//4
  12. Patent, 2443920, 2016//1/2/
  13. Patent, 6804060, 2020/12/04

External Funds

Acceptance Results of Competitive Funds

  1. KAKENHI(Grant-in-Aid for JSPS Research Fellow), 2021, 2022
  2. Science and technology research promotion program for agriculture forestry fisheries and food industry, 2015/04/09, 2016/03/22
  3. 2007
  4. 2010
  5. KAKENHI, 2013, 2014
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  6. KAKENHI, 2012, 2014
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  7. KAKENHI, 2009, 2010
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  8. KAKENHI, Next generation of IVMFC technical development by identification of the microtubule control factor of oocyte., 2009, 2011
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  9. KAKENHI, The study for development of novel in vitro oocyte maturation technique generated from expression profile of micro-RNA during ovulation process, 2009, 2011
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  10. KAKENHI, The study for development of novel mammalian oocyte culture system generated from microarray data base of ovulating process, 2006, 2008
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  11. KAKENHI, 2004, 2005
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  12. KAKENHI, Environmental pollution by sex steroids and assessment for their effects on animals and plants, 2003, 2004
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  13. KAKENHI, Contribution assessment of hatchery origin black sea bream Acanthopagrus schlegeli into natural populations using microsatellite markers, 2002, 2005
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  14. KAKENHI, 2002, 2003
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  15. KAKENHI, 2001, 2002
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  16. KAKENHI, Physiological and biochemical studies on freezability boar spermatozoa, 2000, 2002
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  17. Science and technology research promotion program for agriculture, forestry, fisheries and food industry, 2014/07/02, 2015/03/23
  18. KAKENHI, 2016, 2018
  19. Project for Baby and Infant in Research of healTH and Development to Adolescent and Young adult - BIRTHDAY, 2016/04/01, 2017/03/31
  20. Science and technology research promotion program for agriculture, forestry, fisheries and food industry, 2016/04/01, 2017/03/31
  21. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2017, 2018
  22. Project for Baby and Infant in Research of healTH and Development to Adolescent and Young adult - BIRTHDAY, 2017/04/01, 2018/03/31
  23. Project for Baby and Infant in Research of healTH and Development to Adolescent and Young adult - BIRTHDAY , 2018/04/01, 2019/03/31
  24. KAKENHI(Grant-in-Aid for Scientific Research (B)), 2019, 2021