Takemasa Sakaguchi
Last Updated :2021/01/05
- Affiliations, Positions
- Graduate School of Biomedical and Health Sciences(Medicine), Professor
- E-mail
- tsaka
hiroshima-u.ac.jp
- Other Contact Details
- 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8551, Japan, Japan
TEL : (+81)82-257-5157 FAX : (+81)82-257-5159
Basic Information
Educational Backgrounds
- Nagoya University, Faculty of Medicine, School of Medicine, Japan, 1979/04, 1985/03
- Nagoya University, Graduate School of Medical Sciences, Laboratory of Molecular Pathogenesis, Japan, 1986/04, 1987/03
Academic Degrees
- Doctor of Medicine, Hiroshima University
Research Fields
- Medicine,dentistry, and pharmacy;Basic medicine;Virology
Research Keywords
- Sendai virus (HVJ)
- influenza virus
- hepatitis B virus
- reverse genetics
- Protein three dimensional structure
- morphogenesis
- interferon
- accessory protein
- anti-virus
- persimmon tannin
Educational Activity
Course in Charge
- 2020, Undergraduate Education, Year, Biological Responses
- 2020, Undergraduate Education, 2Term, Introduction for medical research
- 2020, Undergraduate Education, Intensive, Practice for medical research
- 2020, Undergraduate Education, 4Term, Microbiology
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Methods in biomedical sciences
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Methods in biomedical sciences
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Methods in biomedical sciences
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Methods in biomedical sciences
- 2020, Graduate Education (Master's Program) , First Semester, Seminar on health policy & global health
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced seminar on Virology
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced seminar on Virology
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced research on Virology
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced research on Virology
- 2020, Graduate Education (Master's Program) , First Semester, Seminar
- 2020, Graduate Education (Master's Program) , Second Semester, Seminar
- 2020, Graduate Education (Master's Program) , First Semester, Research
- 2020, Graduate Education (Master's Program) , Second Semester, Research
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Research
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
- 2020, Graduate Education (Master's Program) , 3Term, Seminar on Host Defense
- 2020, Graduate Education (Master's Program) , 1Term, Seminar on Health Policy & Global Health
- 2020, Graduate Education (Master's Program) , 1Term, Methods in Biomedical Sciences A
- 2020, Graduate Education (Doctoral Program) , 1Term, Methods in Biomedical Sciences B
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Virology
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Virology
- 2020, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Virology
- 2020, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Virology
Research Activities
Academic Papers
- Structural comparison of the cleavage-activation site of the fusion glycoprotein between virulent and avirulent strains of Newcastle disease virus., Virology, 158(1), 242-247, 19870501
- Structural features unique to each of the three antigenic sites on the hemagglutinin-neuraminidase protein of Newcastle disease virus., Virology, 163(1), 174-182, 19880301
- Identification of amino acids relevant to three antigenic determinants on the fusion protein of Newcastle disease virus that are involved in fusion inhibition and neutralization., Journal of Virology, 62(11), 4427-4430, 19881101
- Newcastle disease virus evolution. I. Multiple lineages defined by sequence variability of the hemagglutinin-neuraminidase gene., Virology, 169(2), 260-272, 19890401
- Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains., Virology, 169(2), 273-282, 19890401
- Endoproteolytic activiation of Newcastle disease virus fusion proteins requires an intracellular acidic environment., Virology, 170(2), 571-574, 19890601
- Immediate protection of mice from lethal wild-type Sendai virus (HVJ) infections by a temperature-sensitive mutant, HVJpi, possessing homologous intefering capacity., Virology, 177(1), 65-74, 19900701
- ★, Identification of endoprotease activity in the trans Golgi membranes of rat liver cells that specifically processes in vitro the fusion glycoprotein precursor of virulent Newcastle disease virus., Virology, 184(2), 504-512, 19911001
- Detection of cytomegalovirus in urine samples by an enzyme-linked immunosorbent assay using a monoclonal antibody against the viral 150-kilodalton protein., Journal of Clinical Microbiology, 30(3), 685-690, 19920301
- Distribution and substrate specificity of intracellular proteolytic processing enzyme(s) for paramyxovirus fusion glycoproteins., Journal of General Virology, 73(3), 583-590, 19920301
- Location and character of the cellular enzyme that cleaves the hemagglutinin of a virulent avian influenza virus., Virology, 190(1), 278-289, 19920901
- Expression of the HN, F, NP and M proteins of Sendai virus by recombinant vaccinia viruses and their contribution to protective immunity against Sendai virus infections in mice., Journal of General Virology, 74, 479-484, 19930301
- Fo-containing noninfectious Sendai virus can initiate replication in mouse lungs but requires a relatively long incubation period., Journal of Virology, 67(12), 7618-7622, 19931201
- A field isolate of Sendai virus: its high virulence to mice and genetic divergence from prototype strains., Arhieves of Virology, 135(1-2), 159-164, 19940101
- Sequence specificity of furin, a proprotein processing endoprotease, for the Hemagglutinin of a virulent avian influenza virus., Journal of Virology, 68(2), 1213-1218, 19940201
- Monitoring for engraftment following rat orthotopic liver transplantation by the polymerase chain reaction., Transplantation Proceedings, 26(2), 902-903, 19940401
- Monitoring for rejection and engraftment by means of the polymerase chain reaction after rat orthotopic liver transplantation., Transplantation, 58(6), 745-748, 19940901
- Correlation of proteolytic cleavage of paramyxovirus F protein precursors with expression of the fur, PACE4 and PC6 genes in mammalian cells., Journal of General Virology, 75(10), 2821-2827, 19941001
- ★, The ion channel activity of the influenza virus M2 protein affects transport through the Golgi apparatus., Journal of Cell Biology, 133(4), 733-747, 19960501
- HLA-DR8 subtyping by polymerase chain reaction (PCR)-DNA conformation polymorphism(DCP) analysis; a simple and practical genotyping method., Hiroshima J. Med. Sci., 45(3), 85-92, 19960901
- Protection of mice from respiratory Sendai virus infections by recombinant vaccinia viruses., Journal of Virology, 71(1), 832-838, 19970101
- ★, Phosphorylation of the Sendai virus M protein is not essential for virus replication either in vitro or in vivo., Virology, 235(2), 360-366, 19970401
- ★, The active oligomeric state of the minimalistic influenza virus M2 ion channel is a tetramer., Proc. Natl. Acad. Sci. USA, 94(10), 5000-5005, 19970501
- Structure and function of the influenza virus M2 ion channel protein., Japanese Journal of Clinical Medicine, 55(10), 89-94, 19971001
- Infulenza virus M2 ion channel-two functions for virus replication., Experimental Medicine, 15(19), 114-118, 19971201
- Viral ion channel : structure and function of the infuluenza virus M2 protein., Virus, 47(2), 177-186, 19971201
- Comparison of substrate specificities against the fusion glycoprotein of virulent Newcastle disease virus between a chick embryo fibroblast processing protease and mammalian subtilisin-like proteases., Microbiology and Immunology, 43(2), 133-140, 19990201
- Double-layered membrane vesicles released from mammalian cells infected with Sendai virus expressing the matrix protein of vesicular stomatitis virus., Virology, 263(1), 230-243, 19990401
- The genome nucleotide sequence of a contemporary wild strain of measles virus and its comparison with the classical Edmonston strain genome., Virology, 256(2), 340-350, 19990401
- Allograft transduction of IL-10 prolongs survival following orthotopic liver transplantation., Gene Therapy, 6(5), 816-822, 19990501
- Double-layered membrane vesicles released from mamalian cells infected with Sendai virus expressing the matrix protein of vesicular stomotitis virus., Virology, 263(1), 230-243, 19991001
- Adenovirus-mediated allograft transduction of Interleukin-10: Role in the induction phase of liver allograft acceptance., Transplantation Proceedings, 32, 247-248, 20000301
- Adenovirus-mediated interleukin-10 overexpression: Comparison between intraportal and intramuscular gene transfer., Transplantation Proceedings, 32(7), 2006, 20000401
- p53 gene therapy for rat liver tumor by intrahepatic arterial injection of AdCMV-p53 in combination with CDDP., Molecular Therapy, 1(5), S326, 20000401
- Effects of adenovirus-mediated IL-10 gene transfer on liver transplantation., Molecular Therapy, 1(5), 176-177, 20000401
- Involvement of the zinc-binding capacity of Sendai virus V protein in viral pathogenesis., Journal of Virology, 74(17), 7834-7841, 20000901
- Adenovirus-mediated interleukin-10 overexpression: Comparison between intraportal and intramuscular gene transfer., Transplantation Proceedings, 32(7), 2006, 20001101
- Conserved and non-conserved regions in the Sendai virus genome: evolution of a gene possessing overlapping reading frames., Virus Genes, 22(1), 47-52, 20010101
- Emergence of anti-inflammatory monocytes in long-term surviving hosts of IL-10-transduced liver allografts., Cytokine, 13(3), 183-187, 20010201
- Differential expression of CD45 isoforms in tolerant and rejecting rat liver transplant recipients., Transplantation Proceedings, 33(1-2), 544, 20010301
- Attenuation of a field Sendai virus isolate through egg-passages is associated with an impediment of viral genome replication in mouse respiratory cells., Archives of Virology, 146(5), 893-908, 20010501
- Matrix proteins of envelope viruses., Virus, 51(1), 11-19, 20010601
- Molecular and epidemiological analyses of human adenovirus type 7 strains isolated from the nationwide outbreak of 1995 in Japan., Journal of Clinical Microbiology, 40(1), 140-145, 20020101
- Alteration of Sendai virus morphogenesis and nucleocapsid incorporation due to mutation of cystein residues of the matrix protein., Journal of Virology, 76(4), 1682-1690, 20020201
- Mutational analysis of the Sendai virus V protein: Importance of the conserved residues for Zn binding, virus pathogenesis, and efficient RNA editing., Virology, 299(2), 172-181, 20020801
- Augmentation of local antitumor immunity in liver by interleukin-2 gene transfer via portal vein., Cancer Gene Therapy, 9(8), 655-664, 20020801
- Involvement of the leader sequence in Sendai virus pathogenesis revealed by recovery of a pathogenic field isolate from cDNA., Journal of Virology, 76(17), 8540-8547, 20020901
- Identification of mutations associated with attenuation of virulence of a field Sendai virus isolate by egg passage., Virus Genes, 25(2), 189-193, 20021001
- Masking of the contribution of V protein to Sendai virus pathogenesis in an infection model with a highly virulent field isolate., Virology, 313(2), 581-587, 20030901
- Safety and growth suppressive effect of intra-hepatic arterial injection of AdCMV p53 combine with CDDP to Rat liver metastatic tumors., Journal of Experimental & Clinical Cancer Research, 22(3), 339-406, 20030901
- Characterization of the amino acid residues of Sendai virus C protein that are critically involved in its interferon antagonism and RNA synthesis down-regulation., Journal of Virology, 78(14), 7443-7454, 20040701
- Paramyxovirus Sendai virus-like particle formation by expression multiple viral proteins and acceleration of its release by C protein., Virology, 325(1), 1-10, 20040701
- Generation of Sendai virus nucleocapsid-like particles in yeast., Virus Research, 108(1-2), 221-224, 20050301
- ★, AIP1/Alix is a binding partner of Sendai virus C protein and facilitates virus budding., Journal of Virology, 79(14), 8933-8941, 20050715
- Cell-specific inhibition of paramyxovirus maturation by proteasome inhibitors., Microbiology and Immunology, 49(9), 835-844, 20050901
- Suppression of interferon-related promoter activation by hepatitis C virus proteins expressed in cultured cells., Hiroshima Journal of Medical Sciences, 55(3), 71-77, 20060901
- Gene delivery of paraoxonase-1 inhibits neointimal hyperplasia after arterial balloon-injury in rabbits fed a high-fat diet., Hypertension Research, 30(1), 85-91, 20070101
- Paramyxovirus Sendai virus V protein counteracts innate virus clearance through IRF-3 activation, but not via interferon, in mice., Virology, 359(1), 82-91, 20070301
- The YLDL sequence within Sendai virus m protein is critical for budding of virus-like particles and interacts with Alix/AIP1 independently of C protein., Journal of Virology, 81(5), 2263-2273, 20070401
- Paramyxovirus budding, Virus, 57(1), 1-7, 20070601
- Persistent and stable gene expression by a cytoplasmic RNA replicon based on a noncytopathic variant Sendai virus., Journal of Biological Chemistry, 282(37), 27383-27391, 20070914
- Recruitment of Alix/AIP1 to the plasma membrane by Sendai virus C protein facilitates budding of virus-like particles., Virology, 371(1), 108-120, 20080101
- Contribution of the leader sequence to homologous viral interference among Sendai virus strains., Virology, 372(1), 64-71, 20080301
- Paramyxovirus Sendai virus C proteins are essential for maintenance of negative-sense RNA genome in virus particles., Virology, 374(2), 495-505, 20080501
- Studies on the paramyxovirus accessory genes by reverse genetics in the Sendai virus?mouse system., Proceedings of the Japan Academy, Series B-Physical and Biological Sciences, 84(10), 439-451, 20090105
- Conserved charged amino acids within Sendai virus C protein play multiple roles in the evasion of innate immune responses., PLoS ONE, 5(5), e10719, 20100501
- Paramyxovirus assembly and budding: building particles that transmit infections., The International Journal of Biochemistry & Cell Biology, 42(9), 1416-1429, 20100901
- Significance of the YLDL motif in the M protein and Alix/AIP1 for Sendai virus budding in the context of virus infection., Virology, 405(2), 334-341, 20100901
- Oncolytic vesicular stomatitis virus administered by isolated limb perfusion suppresses osteosarcoma growth., Journal of Orthopaedic Research, 29(5), 795-800, 20110501
- Decreased expression in nuclear factor-kB essential modulator due to a novel splice-site mutation causes X-linked ectodermal dysplasia with immunodeficiency., Journal of Clinical Immunology, 31(5), 762-772, 20111001
- Analysis of interaction of Sendai virus V protein and melanoma differentiation-associated gene 5., Microbiology and Immunology, 55(11), 760-767, 20111101
- Optineurin with amyotrophic lateral sclerosis-related mutations abrogates inhibition of interferon regulatory factor-3 activation., Neuroscience Letters, 505(3), 279-281, 20111121
- Inhibition of interferon regulatory factor 3 activation by paramyxovirus V protein., Journal of Virology, 86(13), 7136-7145, 20120701
- Inhibition of virus-like particle release of Sendai virus and Nipah virus, but not that of mumps virus, by tetherin/CD317/BST-2., Hiroshima Journal of Medical Sciences, 61(3), 59-67, 20120901
- Passage of a Sendai virus recombinant in embryonated chicken eggs leads to markedly rapid accumulation of U-to-C transitions in a limited region of the viral genome., PLoS One, 7(11), 20121121
- Inactivation of pathogenic viruses by plant-derived tannins: strong effects of extracts from persimmon (Diospyros kaki) on a broad range of viruses., PLoS One, 8(1), 20130125
- The potential of recombinant vesicular stomatitis virus-mediated virotherapy against metastatic colon cancer., International Journal of Molecular Medicine, 31(2), 299-306, 20130201
- Clustered basic amino acids of the small Sendai virus C protein Y1 are critical to its Ran GTPase-mediated nuclear localization., PLoS One, 8(8), 20130809
- Sendai virus C proteins regulate viral genome and antigenome synthesis to dictate the negative genome polarity., Journal of Virology, 88(1), 690-698, 20140101
- Antimicrobial action from a novel porphyrin derivative in photodynamic antimicrobial chemotherapy in vitro., Lasers in Medical Science, 30(1), 383-387, 20150101
- Inactivation of acyclovir-sensitive and -resistant strains of herpes simplex virus type 1 in vitro by photodynamic antimicrobial chemotherapy., Molecular Vision, 21, 532-537, 20150502
- ★, Structural Basis of the Inhibition of STAT1 Activity by Sendai Virus C Protein, Journal of Virology, 89(22), 11487-11499, 20151115
- IFN-beta-inducing, unusual viral RNA species produced by paramyxovirus infection accumulated into distinct cytoplasmic structures in an RNA-type-dependent manner, FRONTIERS IN MICROBIOLOGY, 6, 2015
- Entry Inhibition of Influenza Viruses with High Mannose Binding Lectin ESA-2 from the Red Alga Eucheuma serra through the Recognition of Viral Hemagglutinin, MARINE DRUGS, 13(6), 3454-3465, 2015
- High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120, MARINE BIOTECHNOLOGY, 18(2), 215-231, 201604
- High-Mannose Specific Lectin and Its Recombinants from a Carrageenophyta Kappaphycus alvarezii Represent a Potent Anti-HIV Activity Through High-Affinity Binding to the Viral Envelope Glycoprotein gp120 (vol 18, pg 144, 2016), MARINE BIOTECHNOLOGY, 18(3), 448-448, 2016
- Nonstructural protein p39 of feline calicivirus suppresses host innate immune response by preventing IRF-3 activation, VETERINARY MICROBIOLOGY, 185, 62-67, 2016
- Temporal antimicrobial effect of photodynamic therapy with TONS504 on Pseudomonas aeruginosa, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 57(12), 2016
- Airborne virus detection by a sensing system using a disposable integrated impaction device, JOURNAL OF BREATH RESEARCH, 10(3), 2016
- Structural analysis of the STAT1:STAT2 heterodimer revealed the mechanism of Sendai virus C protein-mediated blockade of type 1 interferon signaling, JOURNAL OF BIOLOGICAL CHEMISTRY, 292(48), 19752-19766, 20171201
- Inactivation of the influenza virus by a supplemental fermented plant product (Manda Koso)., Hiroshima Journal of Medical Sciences, 66(4), 97-101, 201712
- A Single Amino Acid Substitution within the Paramyxovirus Sendai Virus Nucleoprotein Is a Critical Determinant for Production of Interferon-Beta-Inducing Copyback-Type Defective Interfering Genomes, JOURNAL OF VIROLOGY, 92(5), 201805
- Rosmarinic acid is a novel inhibitor for Hepatitis B virus replication targeting viral epsilon RNA-polymerase interaction, PLOS ONE, 13(5), 20180521
- Time-dependent antimicrobial effect of photodynamic therapy with TONS 504 on Pseudomonas aeruginosa, LASERS IN MEDICAL SCIENCE, 33(7), 1455-1460, 201809
- Metastatic tumor cells detection and anti-metastatic potential with vesicular stomatitis virus in immunocompetent murine model of osteosarcoma, JOURNAL OF ORTHOPAEDIC RESEARCH, 36(9), 2562-2569, 201809
- Inactivation of human and avian influenza viruses by potassium oleate of natural soap component through exothermic interaction, PLOS ONE, 13(9), 20180927
- Eligibility of Feline Calicivirus for a Surrogate of Human Norovirus in Comparison with Murine Norovirus, Poliovirus and Coxsackievirus, BIOCONTROL SCIENCE, 23(3), 145-149, 201809
- Novel Near-Infrared Fluorescence-Guided Surgery With Vesicular Stomatitis Virus for Complete Surgical Resection of Osteosarcomas in Mice, JOURNAL OF ORTHOPAEDIC RESEARCH, 37(5), 1192-1201, 201905
- Antifungal efficacy of photodynamic therapy with TONS 504 for pathogenic filamentous fungi, LASERS IN MEDICAL SCIENCE, 34(4), 743-747, 201906
- Development of new concept viral vectors exerting both vaccine and adjuvant activities., IMPACT, 2019(7), 9-11, 2019,8
Publications such as books
- 1997/12, Medical aspects of proteases and protease inhibitors., Cyclic AMP-inducible procalcitonin processing in thyroidal parafollicular cells is regulated by the Kexin family protease., IOS press, 1997, 12, Scholarly Book, Joint work, English, Nagamune H, Muramatsu K, Higashine M, Akamatsu T, Tamai Y, Yonetomi Y, Tsuji A, Izumi K, Sakaguchi T, Yoshida T, Matsuda Y., 978-90-5199-292-2, 206, 12, 22-33
- 2001/06, Recent Research Developments in Virology, Functional divergence of the Paramyxovirinae accessory proteins: the V and C proteins, Transworld Research Network, 2001, 6, Scholarly Book, Joint work, English, Sakaguchi T, Huang C, Kiyotani K, Yoshida T. , 81-86846-99-9, 386, 11, 341-348
- 2011/04, Simple Microbiology Ver. 5, D. Filoviridae, E. Bunyaviridae, F. Arenaviridae., Nankoudo, 2011, 4, Textbook, Joint work, Japanese, Sakaguchi T., 978-4-524-26207-6, 436, 6, 297-302
- 2011/08, Negative Strand RNA virus, Paramyxovirus budding mechanisms., World Scientific Hackensack, NJ., 2011, 8, Scholarly Book, Joint work, English, Harrison MS, Sakaguchi T, Schmitt AP., 981-4317-22-5, 314, 26, 193-218
- 2012/10, Review of Medical Microbiology and Immunology, Eleventh Edition, Warren Levinson, McGraw-Hill Medical (2010/5/19), ISBN: 978-0071700283, Chapter 39, RNA envelope viruses., Maruzen, 2012, 10, Textbook, Joint work, Japanese, Sakaguchi T., 462108562X, 704, 19, 268-286
- 2018/03, Simple Microbiology Ver. 6, D. Filoviridae, E. Bunyaviridae, F. Arenaviridae, Nankodo, 2018, 3, Textbook, Joint work, Japanese, Sakaguchi, T., 978-4-524-25483-5
Patented
- Patent, JP4830075, 2011/09/30
- Patent, JP4848484, 2011/10/28
- Patent, JP5421901, 2013/11/29
- Patent, JP5571577, 2014/07/04
- Patent, JP5593572, 2014/08/15
- Patent, RU:2491929, 2013/09/10
- Patent, CN:ZL201080016783.2, 2013/11/20
- Patent, KR:10-1426744, 2014/07/30