SHIGERU MIYAKI

Last Updated :2024/06/06

Affiliations, Positions
Hiroshima University Hospital(Medical Center for Translational and Clinical Research), Professor (Special Recognition)
E-mail
miyakihiroshima-u.ac.jp
Self-introduction
Currently I am working on a project concerning the expression and function of micro RNAs (miRNAs) in osteoarthritis. This project is highly original as it is the first to address this new class of regulatory RNAs in joint tissue. I have a strong knowledge base in cell and molecular biology and is familiar with a broad range of experimental approaches and models in this field. I have generated mutant mice, established animal models, I am an expert molecular and cell biologist and had in-depth expertise in bioinformatics. My knowledge base not only includes skeletal biology but acquire insight into mechanisms of pathogenesis in musculoskeletal diseases. The goal of proposed studies is to reveal that exosome-mediated extracellular miRNA transfer in joint cell signaling and osteoarthritis pathogenesis. The studies have the potential to reveal important new regulatory pathways in osteoarthritis pathogenesis, and open new insights on osteoarthritis mechanisms. This may lead to the identification of novel therapeutic targets and biomarkers for diagnosis in osteoarthritis. Furthermore, this proposal would not only pave the pathway for understanding and treating osteoarthritis, but it also has the potential to open doors to understanding the general role of extracellular miRNAs as a signaling molecule pathway in a complex network under various physiological and pathophysiological events.

Basic Information

Major Professional Backgrounds

  • 2004/03/01, 2007/06/30, National Center for Child Health and Development, Research Associate
  • 2007/07/01, 2011/03/29, The Scripps Research Institute, Sr. Research Accosiate
  • 2011/03/30, Hiroshima University, Hospital, Jr. Associate professor

Academic Degrees

  • Ph.D., University of Tsukuba

Research Fields

  • Medicine,dentistry, and pharmacy;Clinical surgery;Orthopaedic surgery

Research Keywords

  • Musculoskeletal Biology, Osteoarthritis, miRNAs, Exosomes, Regenerative Medicine, Tendon, Ligament
  • Osteoarthritis

Affiliated Academic Societies

  • Osteoarthritis research society international
  • Orthopaedic Research Society
  • The Japanese Society of Cartilage Metabolism
  • The Japanese society for regenerative medicine
  • The Japanese Society for Bone and Mineral Research

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, Intensive, Practice for medical research
  2. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Orthopaedic Surgery
  3. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Orthopaedic Surgery
  4. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Orthopaedic Surgery
  5. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Orthopaedic Surgery

Research Activities

Academic Papers

  1. Development of a Water-Dispersible Supramolecular Complex of Polyphenol with Polypeptides for Attenuation of the Allergic Response using a Mechanochemical Strategy, MACROMOLECULAR BIOSCIENCE, 23(4), 202304
  2. ★, miR-26a deficiency is associated with bone loss and reduced muscle strength but does not affect severity of cartilage damage in osteoarthritis, MECHANISMS OF AGEING AND DEVELOPMENT, 212, 202306
  3. Deficiency of MicroRNA-23-27-24 Clusters Exhibits the Impairment of Myelination in the Central Nervous System, NEURAL PLASTICITY, 2023, 20230322
  4. The role of substance P on maintaining ligament homeostasis by inhibiting endochondral ossification during osteoarthritis progression, CONNECTIVE TISSUE RESEARCH, 64(1), 82-92, 20230102
  5. ★, miR-23a/b clusters are not essential for the pathogenesis of osteoarthritis in mouse aging and post-traumatic models, FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 10, 20230104
  6. ★, Senescence-accelerated mice prone 8 (SAMP8) in male as a spontaneous osteoarthritis model, ARTHRITIS RESEARCH & THERAPY, 24(1), 20221018
  7. Therapeutic effect of targeting Substance P on the progression of osteoarthritis, MODERN RHEUMATOLOGY, 32(6), 1175-1185, 20221015
  8. ★, Tendon-Specific Dicer Deficient Mice Exhibit Hypoplastic Tendon Through the Downregulation of Tendon-Related Genes and MicroRNAs, FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY, 10, 20220614
  9. ★, The therapeutic capacity of bone marrow MSC-derived extracellular vesicles in Achilles tendon healing is passage-dependent and indicated by specific glycans, FEBS LETTERS, 596(8), 1047-1058, 202204
  10. Pharmacological Targeting of Heme Oxygenase-1 in Osteoarthritis, ANTIOXIDANTS, 10(3), 202103
  11. The Benefit of Minced Cartilage Over Isolated Chondrocytes in Atelocollagen Gel on Chondrocyte Proliferation and Migration, CARTILAGE, 12(1), 93-101, 202101
  12. A Novel Mucidosphaerium sp. Downregulates Inflammatory Gene Expression in Skin and Articular Cells, ALTERNATIVE THERAPIES IN HEALTH AND MEDICINE, 27(1), 40-46, 2021
  13. Role of vasoactive intestinal peptide in the progression of osteoarthritis through bone sclerosis and angiogenesis in subchondral bone, JOURNAL OF ORTHOPAEDIC SCIENCE, 25(5), 897-906, 202009
  14. ★, Histological scoring system for subchondral bone changes in murine models of joint aging and osteoarthritis, SCIENTIFIC REPORTS, 10(1), 20200622
  15. Repair of an Osteochondral Defect With Minced Cartilage Embedded in Atelocollagen Gel: A Rabbit Model, AMERICAN JOURNAL OF SPORTS MEDICINE, 47(9), 2216-2224, 201907
  16. IL-1 Enhances Wnt Signal by Inhibiting DKK1, INFLAMMATION, 41(5), 1945-1954, 201810
  17. Histological Evaluation of Early-Phase Changes in the Osteochondral Unit After Microfracture in a Full-Thickness Cartilage Defect Rat Model, AMERICAN JOURNAL OF SPORTS MEDICINE, 46(12), 3032-3039, 201810
  18. Carnosic acid attenuates cartilage degeneration through induction of heme oxygenase-1 in human articular chondrocytes, EUROPEAN JOURNAL OF PHARMACOLOGY, 830, 1-8, 20180705
  19. ★, Extracellular vesicles in cartilage homeostasis and osteoarthritis, CURRENT OPINION IN RHEUMATOLOGY, 30(1), 129-135, 201801
  20. The delaying effect of alpha-glycerophosphocholine on senescence, transthyretin deposition, and osteoarthritis in senescence-accelerated mouse prone 8 mice, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 82(4), 647-653, 2018
  21. Inhibition of microRNA-222 expression accelerates bone healing with enhancement of osteogenesis, chondrogenesis, and angiogenesis in a rat refractory fracture model, JOURNAL OF ORTHOPAEDIC SCIENCE, 21(6), 852-858, 201611
  22. The role of tetraspanin CD9 in osteoarthritis using three different mouse models, BIOMEDICAL RESEARCH-TOKYO, 37(5), 283-+, 2016
  23. ★, Mesenchymal Stem Cell-Derived Exosomes Promote Fracture Healing in a Mouse Model, STEM CELLS TRANSLATIONAL MEDICINE, 5(12), 1620-1630, 201612
  24. Carnosic acid protects starvation-induced SH-SY5Y cell death through Erk1/2 and Akt pathways, autophagy, and FoxO3a, INTERNATIONAL JOURNAL OF FOOD SCIENCES AND NUTRITION, 67(8), 977-982, 2016
  25. Attenuation of cartilage degeneration by calcitonin gene-related paptide receptor antagonist via inhibition of subchondral bone sclerosis in osteoarthritis mice, JOURNAL OF ORTHOPAEDIC RESEARCH, 34(7), 1177-1184, 201607
  26. ★, Bach1 deficiency reduces severity of osteoarthritis through upregulation of heme oxygenase-1, ARTHRITIS RESEARCH & THERAPY, 17, 20151013
  27. ★, Mesenchymal-stem-cell-derived exosomes accelerate skeletal muscle regeneration, FEBS LETTERS, 589(11), 1257-1265, 20150508
  28. ★, Exosomes from IL-1 beta stimulated synovial fibroblasts induce osteoarthritic changes in articular chondrocytes, ARTHRITIS RESEARCH & THERAPY, 16(4), 2014
  29. ★, Exosome-formed synthetic microRNA-143 is transferred to osteosarcoma cells and inhibits their migration, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 445(2), 381-387, 20140307
  30. Administration of MicroRNA-210 Promotes Spinal Cord Regeneration in Mice, SPINE, 39(14), 1099-1107, 20140615
  31. Circulating microRNAs as biomarkers for evaluating the severity of acute spinal cord injury, SPINAL CORD, 52(8), 596-600, 201408
  32. Analysis of microRNA expression in chondrosarcoma., J Orthop Res, 20131201
  33. Angiogenic microRNA-210 is present in cells surrounding osteonecrosis, JOURNAL OF ORTHOPAEDIC RESEARCH, 30(8), 1263-1270, 201208
  34. ★, Macro view of microRNA function in osteoarthritis, NATURE REVIEWS RHEUMATOLOGY, 8(9), 543-552, 201209
  35. The Effect of Intra-articular Injection of MicroRNA-210 on Ligament Healing in a Rat Model, AMERICAN JOURNAL OF SPORTS MEDICINE, 40(11), 2470-2478, 201211
  36. ★, L-Sox5 and Sox6 enhance chondrogenic miR-140 expression by strengthening dimeric Sox9 activity, J Biol Chem, 287(26), 22206-15, 20120601
  37. Autophagy activation by rapamycin reduces severity of experimental osteoarthritis., Ann Rheum Dis, 20120401
  38. Anterior Cruciate Ligament Changes in Human Joint in Aging and Osteoarthritis., Arthritis and Rheumatology, 20120301
  39. DNA binding-dependent glucocorticoid receptor activity promotes adipogenesis via kruppel-like factor 15 gene expression, Lab Invest, 91(2), 203-215, 2011
  40. Translational suppression of atrophic regulators by miR-23a integrates resistance to skeletal muscle atrophy., J Biol Chem, 20111101
  41. Responses of MicroRNA-124a and 223 following spinal cord injury, Spinal cord, 48(3), 192-196, 2010
  42. The increased expression of histone deacetylase 7 induces matrix metalloproteinase-13 in human knee osteoarthritis., Mod Rheumatol, 20(1), 11-17, 2010
  43. Tissue Neogenesis and STRO-1 Expression in Immature and Mature Articular Cartilage, J Orthop Res, 28(1), 96-102, 2010
  44. ★, microRNA-140 plays dual roles both in cartilage development and homeostasis, Genes Dev, 20100601
  45. The Mohawk homeobox gene plays a critical role in tendon differentiation by regulating type collagen Production., PNAS, 20100601
  46. Extracellular sulfatases support cartilage homeostasis by regulating BMP and FGF signaling pathways., PNAS, 20100601
  47. A systems approach reveals that myogenesis genome network is regulated by the transcriptional repressor RP58., Developmental Cell, 20101201
  48. A disintegrin and metalloproteinase with thorombospondin motifs-9 (ADAMTS-9) expression by chondrocytes during endochondral ossification, Arc Histol Cytol, 72(3), 175-185, 2009
  49. Sox9 directly promotes Bapx1 gene expression to repress Runx2 in chondrocytes, Exp Cell Res, 315(13), 2231-2240, 2009
  50. Mesenchymal progenitor cell markers in human articular cartilage: normal distribution and changes in osteoarthritis, Arthritis Res Ther, 11(3), R85, 2009
  51. Retroviral vector system for fluorescent real-time monitoring of miR-133 during the course of myogenic differentiation, The International Journal of Biochemistry & Cell Biology, 20091101
  52. ★, MicroRNA-140 is expressed in differentiated human articular chondrocytes and modulates IL-1 responses, Arthritis and Rheumatology, 20090901
  53. Expression of microRNA-146 in osteoarthritis cartilage., Arthritis and Rheumatology, 20090401
  54. Induction of apoptosis in the synovium of arthritic mice by intra-articular injection of double stranded microRNA-15a., Arthritis and Rheumatology, 20090901
  55. Characterization of dental pulp stem cells of human tooth germs., J Dent Res, 87(7), 676-681, 2008
  56. ★, Expression of microRNA-146 in rheumatoid arthritis synovial tissue, Arthritis Rheum, 20080501
  57. Use of the SST-REX method for the identification of genes expressed at the condensation stage of chondrogenic cell line ATDC5, J Bone Min Metab, 153-157, 2006
  58. Mechanical stretch inhibits myoblast-to-adipocyte differentiation through Wnt signaling, Biochem Biophys Res Comm, 381-385, 2005
  59. Basic fibroblast growth factor supports chondrogenesis of bone marrow-derived mesenchymal stem cells from patients with osteoarthritis in vitro, Materials Sci Eng, C(24), 403-406, 2004
  60. Identification of membrane and secreted proteins in anterior cruciate ligament derived cells using signal-sequence-trap, a retrovirus-mediated expression screening method, Materials Sci Eng, C(24), 397-401, 2004
  61. Effect of mechanical stretch on TGF-1 expression of C2C12 myogenic cells, Materials Sci Eng, C(24), 387-388, 2001
  62. Mechanical stretch is a down-regulatory signal for differentiation of C2C12 myogenic cells, Materials Sci Eng, C(17), 75-78, 2001
  63. Mechanical stretch in anterior cruciate ligament derived cells regulates type I collagen and decorin expression through extracellular signal-regulated kinase ½ pathway, Materials Sci Eng, C(17), 91-94, 2001

Awards

  1. 2009, Arthritis National Research Foundation (USA), Grant Award
  2. 2010, Orthopaedic Research Society (USA), The New Investigator Recognition Award
  3. 2010, World Congress on Osteoarthritis (OARSI), Young Investigator Award
  4. 2010, Arthritis National Research Foundation, Grant Award

Patented

  1. Patent, 2019/05/31
  2. Patent, 6762007, 2020/09/10

Social Activities

History as Peer Reviews of Academic Papers

  1. Osteoarthritis and Cartilage, Others, Reviewer
  2. Arthritis Research & Therapy, Others, Reviewer
  3. Journal of Orthopaedic Research, Others, Reviewer
  4. Stem Cell Research & Therapy, Others, Reviewer
  5. Cell Communication and Signaling, Others, Reviewer
  6. Scientific Reports, Others, Reviewer
  7. Molecular Therapy, Others, Reviewer
  8. Molecular Therapy - Nucleic Acids, Others, Reviewer
  9. Biochem Biophys Res Commun., Others, Reviewer
  10. Experimental Biology and Medicine, Others, Reviewer
  11. Journal of Experimental Orthopaedics, Others, Reviewer
  12. Food & Function, Others, Reviewer