Hitoshi Komatsuzawa

Last Updated :2025/05/09

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Dentistry & Oral Health Sciences), Professor
E-mail
komatsuzhiroshima-u.ac.jp
Self-introduction
Bacteriology is important for the understanding oral and systemic infectious diseases.Regarding to bacterial research, we are studying 1) Pathogenicity and antibiotic resistance of antibiotic resistant bacteria, 2)Pathogenicity of cariogenic and periodontopathogenic bacteria, 3)Formation of oral microbiome.

Basic Information

Major Professional Backgrounds

  • 1992/10/01, 2000/05/31, Hiroshima University, School of Dentistry, Research Associate
  • 2000/06/01, 2002/03/31, Hiroshima University, School of Dentistry, Associate Professor
  • 2002/04/01, 2008/02/29, Hiroshima University, Graduate School of Biomedical & Health Sciences, Associate Professor
  • 2008/03/01, 2019/04/30, Kagoshima University, Professor
  • 2019/05/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor

Academic Degrees

  • Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Dentistry : Program of Dentistry : Dentistry
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences

Educational Activity

Course in Charge

  1. 2025, Liberal Arts Education Program1, 2Term, General Health and Oral Sciences I
  2. 2025, Undergraduate Education, Year, Biological Responses
  3. 2025, Undergraduate Education, 4Term, Life Science
  4. 2025, Undergraduate Education, 3Term, Microbiology I
  5. 2025, Undergraduate Education, 4Term, Microbiology II
  6. 2025, Undergraduate Education, First Semester, Basic Technique for Microbiology and Immunology
  7. 2025, Undergraduate Education, 4Term, Special Subject
  8. 2025, Undergraduate Education, 3Term, Examination and Diagnosis
  9. 2025, Undergraduate Education, 3Term, Endodontics I
  10. 2025, Undergraduate Education, 4Term, Endodontics II
  11. 2025, Undergraduate Education, 3Term, Periodontology I
  12. 2025, Undergraduate Education, First Semester, Dental Research Practices I
  13. 2025, Undergraduate Education, Second Semester, Dental Research Practices II
  14. 2025, Undergraduate Education, 2Term, Basic and Clinical Integrated Theory
  15. 2025, Undergraduate Education, First Semester, Dental Research Practices III
  16. 2025, Undergraduate Education, 2Term, Dental Research Practices IV
  17. 2025, Undergraduate Education, 1Term, Microbiology and Oral Microbiology
  18. 2025, Undergraduate Education, Second Semester, Practice on Basic Dental Sciences
  19. 2025, Undergraduate Education, 1Term, Microbiology and Oral Microbiology
  20. 2025, Graduate Education (Master's Program) , First Semester, Seminar
  21. 2025, Graduate Education (Master's Program) , Second Semester, Seminar
  22. 2025, Graduate Education (Master's Program) , First Semester, Research
  23. 2025, Graduate Education (Master's Program) , Second Semester, Research
  24. 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  25. 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  26. 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  27. 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  28. 2025, Graduate Education (Master's Program) , 3Term, Seminar on Host Defense
  29. 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Bacteriology
  30. 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Bacteriology
  31. 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Bacteriology
  32. 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Bacteriology

Research Activities

Academic Papers

  1. Opposing genetic polymorphisms of two ABC transporters contribute to the variation of nukacin resistance in Streptococcus mutans, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 90(3), 20240320
    Link to Paper Search Results by DOI
  2. Oral and rectal colonization of methicillin-resistant Staphylococcus aureus in long-term care facility residents and their association with clinical status, MICROBIOLOGY AND IMMUNOLOGY, 68(3), 75-89, 202403
    Link to Paper Search Results by DOI
  3. Isolation of Streptococcus mutans temperate bacteriophage with broad killing activity to S. mutans clinical isolates, ISCIENCE, 26(12), 20231215
    Link to Paper Search Results by DOI
  4. Oral and Rectal Colonization by Antimicrobial-Resistant Gram-Negative Bacteria and Their Association with Death among Residents of Long-Term Care Facilities: A Prospective, Multicenter, Observational, Cohort Study, GERONTOLOGY, 69(3), 261-272, 202303
    Link to Paper Search Results by DOI
  5. Complete genome sequence of cfr (B)-carrying Enterococcus raffinosus isolated from bile in a patient in Japan, JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 34, 43-45, 202309
    Link to Paper Search Results by DOI
  6. Comprehensive Analysis of Bacteriocins Produced by the Hypermucoviscous Klebsiella pneumoniae Species Complex, MICROBIOLOGY SPECTRUM, 11(3), 202306
    Link to Paper Search Results by DOI
  7. Complete genome sequence of optrA-carrying Enterococcus faecalis isolated from open pus in a Japanese patient, JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE, 33, 276-278, 202306
    Link to Paper Search Results by DOI
  8. Different CprABC amino acid sequences affect nisin A susceptibility in Clostridioides difficile isolates, PLOS ONE, 18(1), 20230120
    Link to Paper Search Results by DOI
  9. Periapical lesion following Cnm-positive Streptococcus mutans pulp infection worsens cerebral hemorrhage onset in an SHRSP rat model, CLINICAL AND EXPERIMENTAL IMMUNOLOGY, 210(3), 321-330, 20221231
    Link to Paper Search Results by DOI
  10. ★, Disinfectant Susceptibility of Third-Generation-Cephalosporin/Carbapenem-Resistant Gram-Negative Bacteria Isolated from the Oral Cavity of Residents of Long-Term-Care Facilities, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 89(1), 20230131
    Link to Paper Search Results by DOI
  11. Gene Rearrangement and Modification of Immunity Factors Are Correlated with the Insertion of Bacteriocin Cassettes in Streptococcus mutans, MICROBIOLOGY SPECTRUM, 10(3), 202206
    Link to Paper Search Results by DOI
  12. ★, Complete sequences of epidermin and nukacin encoding plasmids from oral-derived Staphylococcus epidermidis and their antibacterial activity, PLOS ONE, 17(1), 20220118
    Link to Paper Search Results by DOI
  13. Efficiency of Antimicrobial Peptides Against Multidrug-Resistant Staphylococcal Pathogens, FRONTIERS IN MICROBIOLOGY, 13, 20220609
    Link to Paper Search Results by DOI
  14. Novel Virulent Bacteriophage Phi SG005, Which Infects Streptococcus gordonii, Forms a Distinct Clade among Streptococcus Viruses, VIRUSES-BASEL, 13(10), 202110
    Link to Paper Search Results by DOI
  15. Comprehensive characterization of sortase A-dependent surface proteins in Streptococcus mutans, MICROBIOLOGY AND IMMUNOLOGY, 66(3), 145-156, 202203
    Link to Paper Search Results by DOI
  16. ★, Single mutations in BraRS confer high resistance against nisin A in Staphylococcus aureus., MicrobiologyOpen, 17, e791, 20190117
  17. Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans., Microbiology and Immunology, 63(7), 251-260
  18. Heparin-LL37 complexes are less cytotoxic for human dental pulp cells and have undiminished antimicrobial and LPS-neutralizing abilities., Int Endod J, 52(9), 1327-1343
  19. Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans., DNA Res., 25(2), 207-215
  20. Impact of a 7-Day field training on oral health condition in Japan ground self-defense force personnel., Mil Med., 182(7), e1869-e1877
  21. Recombinant Sox enzymes from Paracoccus pantotrophus degrade hydrogen sulfide, a major component of oral malodor., Microbes Environ., 32(1), 54-60
  22. Significant increase of oral bacteria in the early pregnancy period in Japanese women. J Investig, Clin Dent., 8, e12189
  23. Antibacterial effects of glycyrrhetinic acid and its derivatives on Staphylococcus aureus., PLoS One., 11(11), e0165831
  24. Lysine and threonine biosynthesis from aspartate contributes to Staphylococcus aureus growth in calf serum., Appl Environ Microbiol, 82(20), 6150-6157
  25. ★, Two-component systems involved in susceptibility to nisin A in Streptococcus pyogenes. 82(19):5930-5939, 2016, Appl Environ Microbiol., 82(19), 5930-5939
  26. Staphylococcus aureus SrrAB affects susceptibility to hydrogen peroxide and co-existence with Streptococcus sanguinis., PLoS One, 11(7), e0159768
  27. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF-β-regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrinβ1/FAK cascade., Cell Microbiol., 18(12), 1723-1738
  28. C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S. aureus strains. 2016 Mar;60(3):139-147, 2016, Microbiol Immunol., 60(3), 139-147
  29. LL37 induces VEGF expression in dental pulp cells through ERK signalling. 48(7):673-679, Int Endod J., 48(7), 673-679
  30. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect., Peptides, 46, 136-142
  31. Three distinct two-component systems are involved in resistance to the class I bacteriocins, Nukacin ISK-1 and nisin A, in Staphylococcus aureus., PLoS One, 22(8), e69455
  32. Involvement of the novel two-component NsrRS and LcrRS systems in distinct resistance pathways against nisin A and nukacin ISK-1 in Streptococcus mutans., Appl Environ Microbiol., 79(15), 4751-4755
  33. ★, Staphylococcus aureus SasA is responsible for binding to salivary agglutinin, gp340, derived from human saliva., Infect Immun., 81(6), 1870-1879
  34. ★, The S-layer of Tannerella forsythia contributes to serum resistance and oral bacterial co-aggregation., Infect Immun., 81(4), 1198-1206
  35. Antimicrobial peptide LL37 promotes vascular endothelial growth factor-A expression in human periodontal ligament cells., J Periodontal Res., 48(2), 228-234
  36. ★, dpr and sod in Streptococcus mutans are involved in coexistence with S. sanguinis, and PerR is associated with resistance to H2O2., Appl Environ Microbiol., 79(5), 1436-1443
  37. ★, GlmS and NagB regulate amino sugar metabolism in opposing directions and affect Streptococcus mutans virulence., PLoS One, 7(3), e33382
  38. ★, Association of CiaRH with resistance of Streptococcus mutans to antimicrobial peptides in biofilms. 27(2):124-135, 2012, Mol Oral Microbiol., 27(2), 124-135
  39. ★, Growth-phase dependence of susceptibility to antimicrobial peptides in Staphylococcus aureus,　, Microbiology, 157, 1786-1797
  40. Lipopolysaccharides mediate leukotoxin secretion in Aggregatibacter actinomycetemcomitans., Mol Oral Microbiol., 27(2), 70-82
  41. Detection of Fusobacterium nucleatum in chorionic tissues of high-risk pregnant women., J Clin Periodontol., 39(5), 417-424
  42. ★, Expressions of virulence factors in Staphylococcus aureus grown in serum., Appl Environ Microbiol., 77(22), 8097-8105
  43. ★, Bacitracin sensing and resistance in Staphylococcus aureus., FEMS Microbiol Lett., 320(1), 33-39
  44. MK615: a new therapeutic approach for the treatment of oral disease., Med Hypotheses., 77(2), 258-260
  45. Aggregatibacter actinomycetemcomitans Omp29 is associated with bacterial entry to gingival epithelial cells by F-actin rearrangement., PLoS One, 29(6), e18287
  46. Regulatory mechanism for exfoliative toxin production in Staphylococcus aureus. 79(4):1660-1670, 2011, Infect Immun., 79(4), 1660-1670
  47. Additive effects of orexin B and vasoactive intestinal polypeptide on LL-37-mediated antimicrobial activities. 233(1-2):37-45, 2011, J Neuroimmunol., 233(1-2), 37-45
  48. Distinct two-component systems in methicillin-resistant Staphylococcus aureus can change the susceptibility to antimicrobial agents., J Antimicrob Chemother., 65(7), 1536-1537
  49. The antimicrobial peptide LL37 induces the migration of human pulp cells: a possible adjunct for regenerative endodontics. 36(6):1009-1013, 2010, J Endod., 36(6), 1009-1013
  50. Automutanolysin disrupts clinical isolates of cariogenic streptococci in biofilms and planktonic cells., Oral Microbiol Immunol., 24, 451-455
  51. ★, Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus., J Antimicrob Chemother., 61(6), 1266-1269
  52. Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells., Infect Immun., 76, 4783-4791
  53. Irsogladine maleate abolishes the increase in interleukin-8 levels caused by outer membrane protein 29 from Aggregatibacter (Actinobacillus) actinomycetemcomitans through the ERK pathway in human gingival epithelial cells., J Periodontal Res., 43, 508-513
  54. First isolation of blaIMP-7 in a Pseudomonas aeruginosa in Japan. 13(4):276-7, 2007, J Infect Chemother., 13(4), 276-277
  55. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes., Int. Immunol., 19(9), 1095-1102
  56. Cross-reactive adaptive immune response to oral commensal bacteria results in an induction of receptor activator of nuclear factor-kappaB ligand (RANKL)-dependent periodontal bone resorption in a mouse model. Oral Microbiol Immunol. (Mol. Oral Microbiol.), Oral Microbiol Immunol. (Mol. Oral Microbiol.), 22(3), 208-215
  57. Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan., Microbiol Immunol., 51(3), 271-277
  58. Kawai T: Innate immune peptide LL-37 displays distinct expression pattern from beta-defensins in inflamed gingival tissue., Clin Exp Immunol., 146(2), 218-225
  59. ★, Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells., Infect Immun., 74(9), 5211-5220
  60. Identification and molecular characterization of an N-Acetylmuraminidase, Aml, involved in Streptococcus mutans cell separation., Microbiol Immunol., 50(9), 729-742
  61. Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans., Infect Immun., 74(12), 7014-7020
  62. Biogenesis of the Actinobacillus actinomycetemcomitans cytolethal distending toxin holotoxin. 74:3480-3487, 2006, Infect Immun., 74, 3480-3487
  63. Identification and molecular characterization of an N-acetylmuramyl-L-alanine amidase Sle1 involved in cell separation of Staphylococcus aureus. Mol Microbiol. 58(4):1087-1101, 2005, 58(4), 1087-1101
  64. Cell wall-targeting domain of glycylglycine endopeptidase distinguishes among peptidoglycan cross-bridges., J Biol Chem., 28(1), 549-558
  65. Staphylococcus hyicus exfoliative toxins selectively digest porcine desmoglein 1., Microb Pathog., 39(5-6), 171-176
  66. Induction of keratinocyte migration via transactivation of the EGF receptor by the antimicrobial peptide LL37., J Immunol., 175, 4662-4668
  67. Irsogladine maleate influences the response of gap junctional intercellular communication and IL-8 of human gingival epithelial cells following periodontopathogenic bacterial challenge. 333(2):502-507,2005, Biochem Biophys Res Commun., 333(2), 502-507
  68. ★, Susceptibilities of periodontopathogenic and cariogenic bacteria to antibacterial peptides, beta-defensins and LL37, produced by human epithelial cells., J Antimicrob Chemother., 55(6), 888-896
  69. New mechanisms of skin innate immunity: ASK1-mediated keratinocyte differentiation regulates the expression of beta-defensins, LL37, and TLR2., Eur J Immunol., 35(6), 1886-1895
  70. Mutation analysis of the histidine residues in the glycylglycine endopeptidase ALE-1., J Bacteriol., 187(2), 480-487
  71. ★, Reduced content of lysyl-phosphatidylglycerol in the cytoplasmic membrane affects susceptibility to moenomycin, as well as vancomycin, gentamicin, and antimicrobial peptides, in Staphylococcus aureus. 48(12):4800-4807,2004, Antimicrob Agents Chemother., 48(12), 4800-4807
  72. The gate controlling cell wall synthesis in Staphylococcus aureus., Mol Microbiol., 53(4), 1221-1231
  73. Zymographic characterization of bacteriolytic enzymes produced by oral streptococci., Microbiol Immunol., 48(6), 465-469
  74. Syntheses of prostaglandin E2 and E-cadherin and gene expression of beta-defensin-2 by human gingival epithelial cells in response to Actinobacillus actinomycetemcomitans., Inflammation., 27(6), 341-349
  75. ★, Moenomycin-resistance is associated with vancomycin-intermediate susceptibility in Staphylococcus aureus., Microbiol Immunol., 47(12), 927-935
  76. ★, Outer membrane protein 100, a versatile virulence factor of Actinobacillus actinomycetemcomitans., Mol Microbiol., 50(4), 1125-1139
  77. Enhancement of alkaline phosphatase synthesis in pulp cells co-cultured with epithelial cells derived from lower rabbit incisors., Cell Biol Int., 27(10), 815-823
  78. An N-terminal segment of the active component of the bacterial genotoxin cytolethal distending toxin B (CDTB) directs CDTB into the nucleus., J Biol Chem., 278(50), 50671-50681
  79. Macrophage inflammatory protein-3alpha and beta-defensin-2 stimulate dentin sialophosphoprotein gene expression in human pulp cells., Biochem Biophys Res Commun., 306(4), 867-871
  80. ★, Staphylococcus aureus susceptibility to innate antimicrobial peptides, beta-defensins and CAP18, expressed by human keratinocytes., Infect Immun., 71(7), 3730-3739
  81. Prevalence of cytolethal distending toxin production in periodontopathogenic bacteria., J Clin Microbiol., 41(4), 1391-1398
  82. Molecular characterization of an atl null mutant of Staphylococcus aureus., Microbiol Immunol., 46(9), 601-612
  83. Staphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance Mechanism, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(8), 202004
    Link to Paper Search Results by DOI
  84. Antibacterial activity of phellodendron bark against Streptococcus mutans, MICROBIOLOGY AND IMMUNOLOGY, 64(6), 424-434, 202006
    Link to Paper Search Results by DOI
  85. Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans., Microbiol Immunol., 63(7), 251-260, 201907
  86. ★, Staphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance Mechanism, Appl Environ Microbiol, 86 1;86(8):e02923-19.(8), e02923, 20200401
  87. Antibacterial activity of phellodendron bark against Streptococcus mutans., Microbiol Immunol., 64(6), 424-434, 202006
  88. Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification., Anal Biochem., 15(605), 113812, 202009
  89. Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification, ANALYTICAL BIOCHEMISTRY, 605, 20200915
    Link to Paper Search Results by DOI
  90. Biogenesis of the Actinobacillus actinomycetemcomitans cytolethal distending toxin holotoxin, INFECTION AND IMMUNITY, 74(6), 3480-3487, 2006
  91. Identification and molecular characterization of an N-acetylmuraminidase, Aml, involved in Streptococcus mutans cell separation, MICROBIOLOGY AND IMMUNOLOGY, 50(9), 729-742, 2006
  92. Actinobacillus actinomycetemcomitans outer membrane protein 100 triggers innate immunity and production of beta-defensin and the 18-kilodalton cationic antimicrobial protein through the fibronectin-integrin pathway in human gingival epithelial cells, INFECTION AND IMMUNITY, 74(9), 5211-5220, 2006
  93. Single nucleotide polymorphism in the cytolethal distending toxin B gene confers heterogeneity in the cytotoxicity of Actinobacillus actinomycetemcomitans, INFECTION AND IMMUNITY, 74(12), 7014-7020, 2006
  94. Molecular characterization of imipenem-resistant Pseudomonas aeruginosa in Hiroshima, Japan, MICROBIOLOGY AND IMMUNOLOGY, 51(3), 271-277, 2007
  95. Susceptibility of periodontopathogenic and cariogenic bacteria to Defensins and potential therapeutic use of Defensins in oral diseases, CURRENT PHARMACEUTICAL DESIGN, 13(30), 3084-3095, 2007
  96. CXCL16 is a novel mediator of the innate immunity of epidermal keratinocytes, INTERNATIONAL IMMUNOLOGY, 19(9), 1095-1102, 2007
  97. Increased resistance to cationic antimicrobial peptide LL-37 in methicillin-resistant strains of Staphylococcus aureus, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 61(6), 1266-1269, 2008
  98. Cytolethal distending toxin induces caspase-dependent and -independent cell death in MOLT-4 cells, INFECTION AND IMMUNITY, 76(10), 4783-4791, 2008
  99. The Antimicrobial Peptide LL37 Induces the Migration of Human Pulp Cells: A Possible Adjunct for Regenerative Endodontics, JOURNAL OF ENDODONTICS, 36(6), 1009-1013, 2010
  100. Distinct two-component systems in methicillin-resistant Staphylococcus aureus can change the susceptibility to antimicrobial agents, JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY, 65(7), 1536-1537, 2010
  101. Regulatory Mechanism for Exfoliative Toxin Production in Staphylococcus aureus, INFECTION AND IMMUNITY, 79(4), 1660-1670, 2011
  102. Aggregatibacter actinomycetemcomitans Omp29 Is Associated with Bacterial Entry to Gingival Epithelial Cells by F-Actin Rearrangement, PLOS ONE, 6(4), 2011
  103. Additive effects of orexin B and vasoactive intestinal polypeptide on LL-37-mediated antimicrobial activities, JOURNAL OF NEUROIMMUNOLOGY, 233(1-2), 37-45, 2011
  104. Bacitracin sensing and resistance in Staphylococcus aureus, FEMS MICROBIOLOGY LETTERS, 320(1), 33-39, 2011
  105. Growth-phase dependence of susceptibility to antimicrobial peptides in Staphylococcus aureus, MICROBIOLOGY-SGM, 157, 1786-1797, 2011
  106. MK615: A new therapeutic approach for the treatment of oral disease, MEDICAL HYPOTHESES, 77(2), 258-260, 2011
  107. Expression of Virulence Factors by Staphylococcus aureus Grown in Serum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 77(22), 8097-8105, 2011
  108. Role of two-component systems in the resistance of Staphylococcus aureus to antibacterial agents, VIRULENCE, 2(5), 427-430, 2011
  109. Detection of Fusobacterium nucleatum in chorionic tissues of high-risk pregnant women, JOURNAL OF CLINICAL PERIODONTOLOGY, 39(5), 417-424, 2012
  110. GlmS and NagB Regulate Amino Sugar Metabolism in Opposing Directions and Affect Streptococcus mutans Virulence, PLOS ONE, 7(3), 2012
  111. dpr and sod in Streptococcus mutans Are Involved in Coexistence with S. sanguinis, and PerR Is Associated with Resistance to H2O2, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(5), 1436-1443, 2013
  112. The Surface Layer of Tannerella forsythia Contributes to Serum Resistance and Oral Bacterial Coaggregation, INFECTION AND IMMUNITY, 81(4), 1198-1206, 2013
  113. Staphylococcus aureus SasA Is Responsible for Binding to the Salivary Agglutinin gp340, Derived from Human Saliva, INFECTION AND IMMUNITY, 81(6), 1870-1879, 2013
  114. Involvement of the Novel Two-Component NsrRS and LcrRS Systems in Distinct Resistance Pathways against Nisin A and Nukacin ISK-1 in Streptococcus mutans, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 79(15), 4751-4755, 2013
  115. Three Distinct Two-Component Systems Are Involved in Resistance to the Class I Bacteriocins, Nukacin ISK-1 and Nisin A, in Staphyloccus aureus, PLOS ONE, 8(7), 2013
  116. The antimicrobial peptide LL37 promotes bone regeneration in a rat calvarial bone defect, PEPTIDES, 46, 136-142, 2013
  117. C55 bacteriocin produced by ETB-plasmid positive Staphylococcus aureus strains is a key factor for competition with S-aureus strains, MICROBIOLOGY AND IMMUNOLOGY, 60(3), 139-147, 2016
  118. Staphylococcus aureus SrrAB Affects Susceptibility to Hydrogen Peroxide and Co-Existence with Streptococcus sanguinis, PLOS ONE, 11(7), 2016
  119. Two-Component Systems Involved in Susceptibility to Nisin A in Streptococcus pyogenes, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(19), 5930-5939, 2016
  120. Lysine and Threonine Biosynthesis from Aspartate Contributes to Staphylococcus aureus Growth in Calf Serum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 82(20), 6150-6157, 2016
  121. Antibacterial Effects of Glycyrrhetinic Acid and Its Derivatives on Staphylococcus aureus, PLOS ONE, 11(11), 2016
  122. Aggregatibacter actinomycetemcomitans outer membrane protein 29 (Omp29) induces TGF--regulated apoptosis signal in human gingival epithelial cells via fibronectin/integrin1/FAK cascade, CELLULAR MICROBIOLOGY, 18(12), 1723-1738, 2016
  123. Sugar Allocation to Metabolic Pathways is Tightly Regulated and Affects the Virulence of Streptococcus mutans, GENES, 8(1), 2017
  124. Recombinant Sox Enzymes from Paracoccus pantotrophus Degrade Hydrogen Sulfide, a Major Component of Oral Malodor, MICROBES AND ENVIRONMENTS, 32(1), 54-60, 2017
  125. Impact of a 7-Day Field Training on Oral Health Condition in Japan Ground Self-Defense Force Personnel, MILITARY MEDICINE, 182(7), E1869-E1877, 2017
  126. Small RNA repertoires and their intraspecies variation in Aggregatibacter actinomycetemcomitans, DNA RESEARCH, 25(2), 207-215, 2018
  127. Antibacterial activity of disodium succinoyl glycyrrhetinate, a derivative of glycyrrhetinic acid against Streptococcus mutans, MICROBIOLOGY AND IMMUNOLOGY, 63(7), 251-260, 2019
  128. Single mutations in BraRS confer high resistance against nisin A in Staphylococcus aureus, MICROBIOLOGYOPEN, 8(11), 2019
  129. Staphylococcus aureus Virulence Affected by an Alternative Nisin A Resistance Mechanism, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 86(8), 2020
  130. Antibacterial activity of phellodendron bark against Streptococcus mutans, MICROBIOLOGY AND IMMUNOLOGY, 64(6), 424-434, 2020
  131. Rapid detection of the Streptococcus mutans cnm gene by loop-mediated isothermal amplification, ANALYTICAL BIOCHEMISTRY, 605, 2020
  132. Expression of virulence factors under different environmental conditions in Aggregatibacter actinomycetemcomitans, MICROBIOLOGY AND IMMUNOLOGY, 2021
  133. Periodontitis and the outcome of atrial fibrillation ablation: Porphyromonas gingivalis is related to atrial fibrillation recurrence, JOURNAL OF CARDIOVASCULAR ELECTROPHYSIOLOGY, 2021
  134. Comprehensive analysis of bacteriocins in Streptococcus mutans, SCIENTIFIC REPORTS, 11(1), 20210621
    Link to Paper Search Results by DOI
  135. Antibacterial Peptides Resistance in Staphylococcus aureus: Various Mechanisms and the Association with Pathogenicity, GENES, 12(10), 202110
    Link to Paper Search Results by DOI

Publications such as books

  1. 2019/02/20, Oral microbiology and Immunology, Textbook, Compilation, 日本語, ISBN978-4-263-45791-7, 303, 28

Invited Lecture, Oral Presentation, Poster Presentation

  1. Different amino acid sequence for CprABC affects nisin A susceptibility in Clostridioides difficile isolates., Noriaki Ide, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Hiromi Nishi, Hiroyuki Kawaguchi,Hitoshi Komatsuzawa, The 15th Korea-Japan International Symposium on Microbiology, 2022/10/14, Without Invitation, English, Korean Society for Microbiology/Japanese Society for Bacteriology, Yeosu, South Korea
  2. Susceptibility of disinfectants against 3rd-generation Cephalosporin/Carbapenem-resistant Gram-negative bacteria isolated from oral cavity of long-term care facility residents, Haruta Azusa, Matsuo-Kawada-Miki, Yoshikawa Mineka, Takeuchi Maho, Le Nguyen Tra Mi, Tsuga Kazuhiro, Komatsuzawa Hitoshi, The 15th Korea-Japan International Symposium on Microbiology, 2022/10/14, Without Invitation, English, Korean Society for Microbiology/Japanese Soceity for Bacteriology, Yeosu, South Korea
  3. Novel candidates of antimicrobial peptides (AMPs) against MRSA and Resistance mechanisms of MRSA against AMPs., Miki Kawada-Matsuo, Mi Nguyen-Tra Le,and Hitoshi Komatsuzawa, The 15th Korea-Japan International Symposium on Microbiology, 2022/10/14, With Invitation, English, Korean Society for Microbiology/Japanese Society for Bacteriology, Yeosu, South Korea, The establishment and development of antimicrobial agents was one of the most important achievements of the 20th century in terms of dramatically reducing human mortality from infectious diseases. However, in recent years, the emergence of antimicrobial-resistant bacteria (ARB) due to inappropriate use of antibiotics has occurred as a problem and a major clinical challenge due to the limited availability of antibiotics. This raises questions about the usefulness of antibiotics in the 21st century, and research into novel infectious disease treatments to combat these superbugs is needed. Antimicrobial resistant (AMR) organisms of recent concern are diverse, including carbapenem-resistant Enterobacteriaceae, multidrug-resistant Acinetobacter, multidrug-resistant Pseudomonas aeruginosa, methicillin-resistant Staphylococcus aureus (MRSA) and vancomycin-resistant Enterococci (VRE). The O'Neill Report (2014) reported a prediction that by 2050, deaths from ARB will exceed those from cancer. In view of this situation, WHO has proposed an action plan to combat Antimicrobial Resistance (AMR), and Japan has set up an AMR action plan for five years until 2020, and is currently moving to a new phase of AMR control based on this action. Despite these moves to take AMR countermeasures on a global scale, we are still exposed to the threat of ARB, and further countermeasures will be necessary in the future. In this presentation, I will first present trends ARB, including the results of our current ARB analysis. Next, I would like to introduce new antimicrobial candidates for ARB. On AMR action plan in Japan, the development of novel antimicrobial agents has been proposed as one of the measures against ARB, so bacteriophages and antimicrobial peptides (AMPs) are being investigated. In our laboratory, we are investigating the identification and efficacy of biogenic AMPs, including their antimicrobial mechanism against MRSA. For example, bacteriocins, one of the AMPs produced by commensal bacteria, are highly safe because they have high bacterial species specificity and are degraded by proteases present in the intestines of humans and animals, being expected to have little impact on the human body. Therefore, it has already been applied as a biopreservative for foods, and furthermore, it is expected to be used not only in the food area, but also in human and veterinary medicine. Thus, AMPs are attracting attention as candidates for new antimicrobial agents, and I would like to introduce the current research and development status of these peptides and their efficacy against MRSA, including our findings. In the last section, I would like to present some of the concerns regarding the use of AMPs in the treatment of MRSA infections, including our research. To prevent the threat caused by ARB in the future, AMR control measures are very important at present. The treatment of ARB infections with AMPs, which we propose here, could be one of the options for treatments in the future. As AMPs have a different mode of action to currently used antimicrobials, it is expected that the successful use of AMPs and antimicrobials will lead to the development of therapeutic agents against ARB infections and the suppression of ARB emergence.
  4. Different amino acid sequence for CprABC affects nisin A susceptibility in Clostridioides difficile isolates., Noriaki Ide, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Hiromi Nishi, Hiroyuki Kawaguchi and Hitoshi Komatsuzawa, The 15th Korea-Japan International Symposium on Microbiology, 2022/10/14, Without Invitation, English, Korean Society for Microbiology/Japanese Soceity for Bacteriology, Yeosu, South Korea
  5. Different amino acid sequence for CprABC affects nisin A susceptibility in Clostridioides difficile isolates., Noriaki Ide, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Hiromi Nishi, Hiroyuki Kawaguchi, Hitoshi Komatsuzawa, The 15th Korea-Japan International Symposium on Microbiology, 2022/10/14, Without Invitation, English, Korean Society for Microbiology/Japanese Soceity for Bacteriology, Yeosu, South Korea
  6. A Novel Bacteriocin Resistance Mechanism Mediated by Cell Surface Charge in Staphylococcus aureus, Yujin Suzuki, Miki Kawada-Matsuo, Mi Nguyen Tra Le, Hitoshi Komatsuzawa, The 96th Annual Meeting of Japanese Society for Bacteriology, 2023/03/16, Without Invitation, Japanese, Japanese Society for Bacteriology, Himeji, Japan
  7. Different CprABC aminoacid sequences affect nisinA susceptibility in Clostridioides difficile, Noriaki Ide, Miki Matsuo, Mi Nguyen Tra Le, Junzo Hisatsune,Toshinori Hara, Seiya Kashiyama, Michiya Yokozaki, Hiroki Ohge,Motoyuki Sugai, Hitoshi Komatsuzawa, The 96th Annual Meeting of Japanese Society for Bacteriology, 2023/03/16, Without Invitation, Japanese
  8. Isolation and characterization of drug-resistant bacteria from nasal and oral cavities, Tomoki Kawayanagi, Miki Matsuo, Mi Nguyen Tra Le, Toru Takeshita, Junzo Hisatsune, Satoru Kusaka, Ryota Nomura, Hideki Shiba, Motoyuki Sugai, Hitoshi Komatsuzawa, The 96th Annual Meeting of Japanese Society for Bacteriology, 2023/03/16, Without Invitation, Japanese, Japanese Society for Bacteriology, himeji, Japan
  9. Analysis of antimicrobial susceptibility and drug resistance genes of Enterococci, Ayumi Fujii, Miki Matsuo, Kanako Masuda, Junzo Hisatsune, KayokoTadera, Seiya Kashiyama, Michiya Yokozaki, Tomonao Aikawa, Hiroki Ohge, Hitoshi Komatsuzawa, The 96th Annual Meeting of Japanese Society for Bacteriology., 2023/03/16, Without Invitation, Japanese, Japanese Society for Bacteriology, Himeji
  10. Phage search for prevention and treatment of oral infections, Miki Kawada-Matsuo, Hitoshi Komatsuzawa, The 96th Annual Meeting of Japanese Society for Bacteriology, 2023/03/16, With Invitation, Japanese, Japanese Society for Bacteriology, Himeji
  11. Complete sequences of bacteriocin plasmids of S. epidermidis and their antibacterial activity, 2022/03/29, Without Invitation, Japanese
  12. Identification of a new resistance factor against nukacins in Streptococcus mutans, Miki Kawada-Matsuo, Mi Nguyen-Tra Le, Hitoshi Komatsuzawa, 2021/10/10, Without Invitation, Japanese
  13. Isolation and characterization of oral antibiotic-resistant Gram-negative bacteria in the long-term care facility, Azusa Haruta, Miki Matsuo, Mineka Yoshikawa, Maho Takeuchi, Le Nguyen Tra Mi, Koji Yahara, Toshiki Kajihara, Syoko Kutsuno, Hiroki Ohge, Kazuhiro Tsuga, Motoyuki Sugai, Hitoshi Komatsuzawa, MIRAI 2.0 Digital Research and Innovation Week, 2021/06/07, Without Invitation, English
  14. Isolation and characterization of oral antibiotic-resistant Gram-negative bacteria in the long-term care facility, Azusa Haruta, Miki Matsuo, Mineka Yoshikawa, Maho Takeuchi, Le Nguyen Tra Mi, Koji Yahara, Toshiki Kajihara, Syoko Kutsuno, Hiroki Ohge, Kazuhiro Tsuga, Motoyuki Sugai, Hitoshi Komatsuzawa, The 6th JSMid, 2021/08/01, Without Invitation, English
  15. Evaluation of high resistant mechanism against nisin A in nisin A high resistant MRSA, 2019/10/12, Without Invitation, Japanese, preprint
  16. Effect of plant-derived extract , Phellodendron bark, against cariogenic bacteria, 2020/02/21, Without Invitation, Japanese, preprint
  17. Characterization of bacteriocin-KO mutants in S. mutans, 2020/02/21, Without Invitation, Japanese, preprint
  18. Skillful survival strategies with two-component systems of commensal bacteria against human- and bacterial-derived antimicrobial peptides, 2020/02/21, With Invitation, Japanese, preprint
  19. Survival strategy of Staphylococcus aureus in the host, Komatsuzawa H, 2019/10/04, With Invitation, Japanese, preprint

External Funds

Acceptance Results of Competitive Funds

  1. Research Program on Emerging and Re-emerging Infectious Diseases, 2022//0/4/, 2023//0/3/
  2. Research Program on Emerging and Re-emerging Infectious Diseases, 2021/04/01, 2022/03/31
  3. Development of novel prevention method for dental caries by bacteriophage, 2020, 2021
  4. Grants-in-Aid for Scientific Research, 2016, 2018
  5. Grants-in Aid for Scientific research (C), 2013, 2015
  6. Grant-in-Aid for Challenging Exploratory Research, 2010, 2011
  7. 2007, 2008
  8. 2007, 2008
  9. KAKENHI(Grant-in-Aid for Challenging Research (Exploratory)), 2020, 2021