Naoki Isobe

Last Updated :2024/02/01

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
nisohiroshima-u.ac.jp
Other Contact Details
1-4-4 HIGASHI-HIROSHIMA, Japan
TEL : (+81)82-424-7993 FAX : (+81)82-424-7993
Self-introduction
My research aims to prevent mastitis in dairy cows. I found that various antimicrobial components are synthesized in different sites of udder and that they cooperatively remove pathogens. I proposed method decreasing frequency of milking for new mastitis treatment. I elucidated that mastitis can be caused by not only viable bacteria but also bacterial components translocated from other organs.

Basic Information

Major Professional Backgrounds

  • 1996/08/01, 2005/03/31, Hiroshima University, Graduate School for International Development and Cooperation, Research Associate
  • 2016/09/26, 2017/09/26, Gadjah Mada University, Faculty of Agriculture, Visiting professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Biophere Sciences, Japan, 1993/04, 1996/03
  • Hiroshima University, Faculty of Applied Biological Science, Japan, 1987/04, 1991/03

Academic Degrees

  • Doctor of Agriculture, Hiroshima University
  • Master of Agriculture, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Applied Biological Science : Department of Applied Biological Science : Applied Animal and Plant Science Program
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Bioresource Science

In Charge of Primary Major Programs

  • Applied Animal and Plant Science Program

Research Fields

  • Agricultural sciences;Animal life science;Animal production science

Research Keywords

  • mammary gland
  • EIA
  • Somatic cell count
  • immunohistochemistry
  • hormone
  • milk
  • feces
  • Mastitis
  • Innate immunity
  • Antimicrobial peptide

Affiliated Academic Societies

  • Japanese Society of Veterinary Science, 2016
  • The American Dairy Science Association, 2009
  • Japanese Association of Animal Science, 1990
  • Japanese Association of Animal Reproduction, 1993
  • Japanese Association of Mastitis, 2006
  • Kansai Association of Animal Science, 1990
  • Chushikoku Mastitis Council, 2013

Educational Activity

Course in Charge

  1. 2023, Liberal Arts Education Program1, 1Term, Introduction to Applied Biological Sciences toward SDGs
  2. 2023, Undergraduate Education, 1Term, Introduction to Applied Biological Sciences
  3. 2023, Undergraduate Education, Intensive, Laboratory Work in General Biology II
  4. 2023, Undergraduate Education, 1Term, Introduction to Physiology
  5. 2023, Undergraduate Education, 3Term, Animal Functional Anatomy
  6. 2023, Undergraduate Education, 1Term, Animal Physiology and Production
  7. 2023, Undergraduate Education, Intensive, Laboratory and Field Works in Animal Production I
  8. 2023, Undergraduate Education, Second Semester, Graduation Thesis I
  9. 2023, Undergraduate Education, First Semester, Graduation Thesis II
  10. 2023, Undergraduate Education, Second Semester, Graduation Thesis III
  11. 2023, Undergraduate Education, Intensive, Life, food and agricultural education through experimental studies of dairy farming
  12. 2023, Undergraduate Education, Intensive, (AIMS) Introductory Physiology of Domestic Animals
  13. 2023, Undergraduate Education, 3Term, Introductory Physiology of Domestic Animals
  14. 2023, Graduate Education (Master's Program) , 1Term, Exercises in Bioresource Science A
  15. 2023, Graduate Education (Master's Program) , 2Term, Exercises in Bioresource Science A
  16. 2023, Graduate Education (Master's Program) , 3Term, Exercises in Bioresource ScienceB
  17. 2023, Graduate Education (Master's Program) , 4Term, Exercises in Bioresource ScienceB
  18. 2023, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Bioresource Science
  19. 2023, Graduate Education (Master's Program) , 2Term, Animal Production Science I
  20. 2023, Graduate Education (Master's Program) , 4Term, Animal Production Science II
  21. 2023, Graduate Education (Master's Program) , 3Term, Smart Livestock Farming
  22. 2023, Graduate Education (Doctoral Program) , Intensive, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. Menthol application on healthy and inflamed goat udders changes antimicrobial components in milk, ANIMAL SCIENCE JOURNAL, 94(1), e13832, 202301
  2. Valine Treatment Enhances Antimicrobial Component Production in Mammary Epithelial Cells and the Milk of Lactating Goats Without Influencing the Tight Junction Barrier, JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 28(1), 202312
  3. Effects of Newcastle Disease/Infectious Bronchitis Vaccine and Feeding Yeast Products on the Innate Immune System in the Proventriculus and Ileum of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 60(1), 2023005, 202301
  4. Investigating mammary glands of lactating goats for the presence of tertiary lymphoid organs, FRONTIERS IN IMMUNOLOGY, 13, 941333, 20220810
  5. Effect of 6-n-propyl-2-thiouracil or dexamethasone administration on the responses of antimicrobial components in goat milk to intramammary lipopolysaccharide infusion, ANIMAL SCIENCE JOURNAL, 93(1), 202201
  6. Effects of ozonized glycerin on inflammation of mammary glands induced by intramammary lipopolysaccharide infusion in goats, ANIMAL SCIENCE JOURNAL, 93(1), 202201
  7. Effects of Newcastle disease and infectious bronchitis vaccine and yeast products feeding on the innate immune system in the proventriculus and ileum of broiler chicks ., Journal of Poultry Science, 20220000
  8. Differences in antimicrobial components between bacterial culture-positive and -negative bovine clinical mastitis milk., Animal Science Journal, 93, e13771, 20220601
  9. Investigating mammary glands of lactating goats for the presence of tertiary lymphoid organs., Frontiers in Immunology, 13, 941333, 20220810
  10. Sodium acetate and sodium butyrate differentially upregulate antimicrobial component production in mammary glands of lactating goats., Journal of Mammary Gland Biology and Neoplasia, 2022
  11. Effects of frequent teat stimulation on antimicrobial component production in mammary glands of lactating goats., Veterinary Immunology and Immunopathology, 249, 110431, 20220701
  12. Modulation of the innate immune system by lipopolysaccharide in the proventriculus of chicks inoculated with or without Newcastle disease and infectious bronchitis vaccine., Poultry Science, 101(4), 101719, 20220401
  13. Adverse effects of heat stress on mammary alveolar development in vitro., Journal of Mammary Gland Biology and Neoplasia, 2022
  14. Local Heat Treatment of Goat Udders Influences Innate Immune Functions in Mammary Glands, JOURNAL OF MAMMARY GLAND BIOLOGY AND NEOPLASIA, 26(4), 387-397, 202112
  15. Effect of oral administration of colostrum on inflammation in the udders of dairy cows suffering from mastitis, JOURNAL OF VETERINARY MEDICAL SCIENCE, 84(1), 59-63, 202201
  16. Slight Disruption in Intestinal Environment by Dextran Sodium Sulfate Reduces Egg Yolk Size Through Disfunction of Ovarian Follicle Growth, FRONTIERS IN PHYSIOLOGY, 11, 20210115
  17. Immune response during the onset of coliform mastitis in dairy cows vaccinated with STARTVAC (R), ANIMAL SCIENCE JOURNAL, 92(1), 2021
  18. Association of endometritis and ovarian follicular cyst with mastitis in dairy cows., The Journal of Veterinary Medical Science, 83(2), 338-343, 2021
  19. ★, Effect of temporary cessation of milking on the innate immune components in goat milk., Journal of Dairy Science, 104(9), 10374-10381, 20210901
  20. Concentration patterns of antibacterial factors and immunoglobulin A antibody in foremilk fractions of healthy cows., Animal Science Journal, 91(1), e13372., 2020
  21. Effect of oral administration of colostrum whey to peripartum goat on antimicrobial peptides in postpartum milk., Animal Science Journal, 91(1), e13365, 2020
  22. Seasonal variations in the concentration of antimicrobial components in milk of dairy cows., Animal Science Journal, 91, e13427, 2020
  23. Effects of intrauterine infusion of bacterial lipopolysaccharides on the mammary gland inflammatory response in goats, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 219, 202001
  24. Effects of Oral Administration of Lactobacillus reuteri on Mucosal Barrier Function in the Digestive Tract of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 57(1), 67-76, 202001
  25. Translocation of intrauterine-infused bacterial lipopolysaccharides to the mammary gland in dexamethasone-treated goats, Reproduction in Domestic Animals, 2020
  26. Outcome prediction from the first examination in clinical mastitis using ultrasonography in dairy cows., Animal Science Journal, 91(1), e13452, 2020
  27. Short communication: Production of antimicrobial peptide S100A8 in the goat mammary gland and effect of intramammary infusion of lipopolysaccharide on S100A8 concentration in milk, JOURNAL OF DAIRY SCIENCE, 102(5), 4674-4681, 201905
  28. Age-related modulation of the isthmic and uterine mucosal innate immune defense system in laying hens, POULTRY SCIENCE, 98(7), 3022-3028, 201907
  29. Effects of the routine multiple vaccinations on the expression of innate immune molecules and induction of histone modification in ovarian cells of layer chicks, POULTRY SCIENCE, 98(10), 5127-5136, 201910
  30. Blood Testosterone Concentration and Testosterone-induced Aggressive Behavior in Male Layer Chicks: Comparison between Isolated- and Grouped-Raising, JOURNAL OF POULTRY SCIENCE, 56(4), 290-297, 201910
  31. Effects of Salmonella enteritidis Vaccination on the Expression of Innate Immune Molecules and Histone Modifications in the Follicular Theca of Laying Hens, JOURNAL OF POULTRY SCIENCE, 56(4), 298-307, 201910
  32. Association between bovine leukemia virus proviral load and severity of clinical mastitis, JOURNAL OF VETERINARY MEDICAL SCIENCE, 81(10), 1431-1437, 201910
  33. Detection of APAF1 mutation in Holstein cows and mummified foetuses in Japanese dairy herds, REPRODUCTION IN DOMESTIC ANIMALS, 53(1), 137-142, 201802
  34. Microcystin-leucine-arginine Modulates the Expression Patterns of Proinflammatory Cytokines and an Apoptotic Gene in Chicken Liver, JOURNAL OF POULTRY SCIENCE, 55(1), 70-77, 201801
  35. Blood ionized calcium levels and acute-phase blood glucose kinetics in goats after intramammary infusion of lipopolysaccharide, JOURNAL OF VETERINARY MEDICAL SCIENCE, 80(2), 242-246, 201802
  36. Innate antiviral immune response against infectious bronchitis virus and involvement of prostaglandin E2 in the uterine mucosa of laying hens, THERIOGENOLOGY, 110, 122-129, 20180401
  37. Effects of Interleukin-1 beta and-6 on the Expression of Ion Transporters Involved in Eggshell Mineralization in Cultured Hen Uterine Mucosal Tissue, JOURNAL OF POULTRY SCIENCE, 55(2), 142-149, 201804
  38. Changes in plasma concentrations of S100A7 and S100A8 in dairy cows during pregnancy, REPRODUCTION IN DOMESTIC ANIMALS, 53(4), 1013-1015, 201808
  39. Effects of colostrum whey on immune function in the digestive tract of goats, ANIMAL SCIENCE JOURNAL, 89(8), 1152-1160, 201808
  40. Investigation of the binding of goat cathelicidin-7 to lipopolysaccharide and leucocidal suppression of pro-inflammatory cytokines., Small Ruminant Research, 168:, 101-106, 2018
  41. Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows, ANIMAL SCIENCE JOURNAL, 88(3), 484-488, 2017
  42. Effects of inhibitors of transcription factors, nuclear factor-kappa B and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells, POULTRY SCIENCE, 96(3), 723-730, 2017
  43. Control mechanisms for producing antimicrobial factors in ruminant mammary gland, ANIMAL SCIENCE JOURNAL, 88(7), 937-943, 2017
  44. Effect of steroid hormones on the innate immune response induced by Staphylococcus aureus in the goat mammary gland, REPRODUCTION IN DOMESTIC ANIMALS, 52(4), 579-584, 2017
  45. Changes in the concentrations of somatic cell counts, lingual antimicrobial peptide and lactoperoxidase activity in milk at periovulatory period in dairy cows., Animal Science Journal, 88(3), 484-488, 20170301
  46. Effect of nutrient levels during the far-off period on postpartum productivity in dairy cows., Animal Science Journal, 2017
  47. The extension of raw milk quality during storage through supplementation of hydrocyanic acid from fresh cassava peel in dairy cattle diet. Songklanakarin Journal of Science and Technology (in press), Songklanakarin Journal of Science and Technology, 2017
  48. The inhibitory effect of sodium thiocyanate and sodium percarbonate rations on microorganism growth in raw milk samples as an effective treatment to extend milk quality during storage., Songklanakarin Journal of Science and Technology, 39(1), 2017
  49. Effects of dietary levels of fresh cassava pulp in dairy cattle diet on productive performance and keeping quality of raw milk. Songklanakarin Journal of Science and Technology (in press), Songklanakarin Journal of Science and Technology, 2017
  50. Microcystin-LR modulates the expression patterns of proinflammatory cytokines and apoptotic gene in the liver of chicken., Journal of Poultry Science, 2017
  51. Cellular and soluble components decrease the viable pathogen counts in milk from dairy cows with subclinical mastitis., The Journal of Veterinary Medical Science, 79(8), 1389-1393, 201708
  52. Effects of Probiotics on the Expression of Cathelicidins in Response to Stimulation by Salmonella Minnesota Lipopolysaccharides in the Proventriculus and Cecum of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 53(4), 298-304, 2016
  53. Change in viable bacterial count during preservation of milk derived from dairy cows with subclinical mastitis and its relationship with antimicrobial components in milk., The Journal of Veterinary Medical Science, 78(8), 1245-1250, 201608
  54. Expression of lipases and lipid receptors in sperm storage tubules and possible role of fatty acids in sperm survival in the hen oviduct., Theriogenology, 85, 1334-1342, 20160415
  55. Effects of viral-associated molecular patterns on the expression of cathelicidins in the hen vagina., Journal of Poultry Science, 2016
  56. The inhibitory effect of sodium thiocyanate and sodium percarbonate rations on microorganism growth in raw milk samples as an effective treatment to extend milk quality during storage., Songklanakarin Journal of Science and Technology, 39(1), 2016
  57. Effects of inhibitors of transcription factors, nuclear factor-κB and activator protein 1, on the expression of proinflammatory cytokines and chemokines induced by stimulation with Toll-like receptor ligands in hen vaginal cells, Poultry Science, 2016
  58. The effect of estrogen on the early cytotoxic response to IB virus infection in hen oviduct, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 164(42737), 56-66, 20150315
  59. Dynamics of lingual antimicrobial peptide, lactoferrin concentrations and lactoperoxidase activity in the milk of cows treated for clinical mastitis, ANIMAL SCIENCE JOURNAL, 86(2), 153-158, 201502
  60. Effects of Probiotics on the Expression and Localization of Avian beta-defensins in the Proventriculus of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 52(1), 57-67, 201501
  61. Expression of cathelicidins mRNA in the goat mammary gland and effect of the intramammary infusion of lipopolysaccharide on milk cathelicidin-2 concentration, VETERINARY MICROBIOLOGY, 170(42737), 125-134, 20140514
  62. Effects of different TLR ligands on the expression of proinflammatory cytokines and avian beta-defensins in the uterine and vaginal tissues of laying hens, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 162(42798), 132-141, 20141215
  63. Innate immune functions in hen reproductive organs, AVIAN BIOLOGY RESEARCH, 7(1), 39-47, 2014
  64. Effects of calcium salts of medium-chain fatty acids on plasma metabolite and hormone concentrations in early lactating dairy cows, ANIMAL PRODUCTION SCIENCE, 54(10), 1699-1702, 2014
  65. Messenger RNA expression and immunolocalization of psoriasin in the goat mammary gland and its milk concentration after an intramammary infusion of lipopolysaccharide, VETERINARY JOURNAL, 202(1), 89-93, 2014
  66. Effects of avian infectious bronchitis virus antigen on eggshell formation and immunoreaction in hen oviduct, THERIOGENOLOGY, 81(8), 1129-1138, 2014
  67. Toll-like receptor signaling for the induction of mucin expression by lipopolysaccharide in the hen vagina, POULTRY SCIENCE, 93(3), 673-679, 2014
  68. Relationship between the somatic cell count in milk and reproductive function in peripartum dairy cows, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 60(6), 433-437, 2014
  69. Relationship between concentration of lingual antimicrobial peptide and somatic cell count in milk of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 153(42798), 298-301, 20130615
  70. Expression of tight junction molecule claudins" in the lower oviductal segments and their changes with egg-laying phase and gonadal steroid stimulation in hens", THERIOGENOLOGY, 79(2), 211-218, 20130115
  71. Comparison of cadherin and integrin localization in bovine cystic and healthy follicles, ANIMAL SCIENCE JOURNAL, 84(4), 303-309, 2013
  72. Dectin-1 gene polymorphism is associated with susceptibility to nonspecific digestive disorders and cytokine expression in rabbits, JOURNAL OF ANIMAL SCIENCE, 91(9), 4051-4059, 2013
  73. Lingual antimicrobial peptide and lactoferrin concentrations and lactoperoxidase activity in bovine colostrum are associated with subsequent somatic cell count, ANIMAL SCIENCE JOURNAL, 84(11), 751-756, 2013
  74. Induction of avian beta-defensins by CpG oligodeoxynucleotides and proinflammatory cytokines in hen vaginal cells in vitro, REPRODUCTION, 145(6), 621-631, 2013
  75. Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 50(3), 275-281, 2013
  76. Effects of Lipopolysaccharide on the Recruitment of T Cells in the Seminal Tract of Roosters, JOURNAL OF POULTRY SCIENCE, 50(1), 68-73, 2013
  77. Effects of Repeated Lipopolysaccharide Stimulation on the Development of Antigen-presenting Cells and T Cells Pool in Hen Vagina, JOURNAL OF POULTRY SCIENCE, 50(1), 83-89, 2013
  78. Induction of mucin expression by estrogen and lipopolysaccharide in the lower oviductal segments in hens, POULTRY SCIENCE, 92(12), 3205-3213, 2013
  79. Relationship between concentration of lingual antimicrobial peptide and somatic cell count in milk of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 153(3-4), 298-301, 2013
  80. Effects of Probiotics on the Localization of T Cell Subsets in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 50(3), 275-281, 2013
  81. Expression of Toll-like receptors and effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokine in the testis and epididymis of roosters, POULTRY SCIENCE, 91(8), 1997-2003, 2012
  82. Differences in the mucosal surface barrier formed by mucin in the lower oviductal segments between laying and molting hens, POULTRY SCIENCE, 91(5), 1173-1178, 20120501
  83. Differential immunolocalization between lingual antimicrobial peptide and lactoferrin in mammary gland of dairy cows, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 145(42737), 499-504, 20120115
  84. Effect of enterotoxigenic Escherichia coli vaccine on innate immune function of bovine mammary gland infused with lipopolysaccharide, JOURNAL OF DAIRY SCIENCE, 95(9), 5067-5074, 2012
  85. Effects of lipopolysaccharide and interleukins on the expression of avian beta-defensins in hen ovarian follicular tissue, POULTRY SCIENCE, 91(11), 2877-2884, 2012
  86. Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and influx of leukocytes in the hen ovary, POULTRY SCIENCE, 90(9), 2054-2062, 20110901
  87. Effects of lipopolysaccharide on the expression of proinflammatory cytokines and chemokines and the subsequent recruitment of immunocompetent cells in the oviduct of laying and molting hens, POULTRY SCIENCE, 90(10), 2332-2341, 20111001
  88. Differential localization of lingual antimicrobial peptide in the digestive tract mucosal epithelium of calves, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 142(42737), 87-94, 20110715
  89. Expression of Toll-like receptors and avian beta-defensins and their changes in response to bacterial components in chicken sperm, POULTRY SCIENCE, 90(2), 417-425, 20110201
  90. Detection of Avian Beta-defensins mRNA and Proteins in Male Reproductive Organs in Chicken, JOURNAL OF POULTRY SCIENCE, 48(4), 275-280, 2011
  91. Changes in the Density of Immunoreactive Avian beta-Defensin-3 and-11 in the Hen Uterus in Response to Lipopolysaccharide Inoculation, JOURNAL OF POULTRY SCIENCE, 48(1), 73-77, 2011
  92. Lactoperoxidase activity in milk is correlated with somatic cell count in dairy cows, JOURNAL OF DAIRY SCIENCE, 94(8), 3868-3874, 2011
  93. Follicular Persistence Induced by Adrenocorticotropic Hormone Administration in Goats, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 57(2), 212-216, 2011
  94. Analysis of Changes in the Expression of Transforming Growth Factor-beta s in the Utero-vaginal Junction of Hen Oviduct in Response to Sperm Concerning Their Significance in Sperm Survivability, JOURNAL OF POULTRY SCIENCE, 47(4), 326-332, 2010
  95. Effects of Feeding Probiotics on the Localization of Cells Containing Immunoreactive Interleukin-6 in the Intestine of Broiler Chicks, JOURNAL OF POULTRY SCIENCE, 47(3), 250-255, 2010
  96. Changes in the Localization of Immunoreactive Avian Beta-Defensin-8,-10 and-12 in Hen Ovarian Follicles during Follicular Growth, JOURNAL OF POULTRY SCIENCE, 47(1), 77-84, 2010
  97. Expression of Toll-like receptors (TLRs) and TLR4 response to lipopolysaccharide in hen oviduct, VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY, 127(42798), 259-268, 20090215
  98. Co-transfer of parthenogenotes and single porcine embryos leads to full-term development of the embryos, ANIMAL REPRODUCTION SCIENCE, 112(42737), 8-21, 2009
  99. Changes in the expression of interleukin-1 beta and lipopolysaccharide-induced TNF factor in the oviduct of laying hens in response to artificial insemination, REPRODUCTION, 137(3), 527-536, 2009
  100. Existence of functional lingual antimicrobial peptide in bovine milk, JOURNAL OF DAIRY SCIENCE, 92(6), 2691-2695, 2009
  101. Identification of Ghrelin in Fertilized Eggs of Chicken, JOURNAL OF POULTRY SCIENCE, 46(3), 257-259, 2009
  102. Intramammary challenge of lipopolysaccharide stimulates secretion of lingual antimicrobial peptide into milk of dairy cows, JOURNAL OF DAIRY SCIENCE, 92(12), 6046-6051, 2009
  103. Immunolocalization of avian beta-defensins in the hen oviduct and their changes in the uterus during eggshell formation, REPRODUCTION, 138(6), 971-978, 2009
  104. ★, Existence of Functional Lingual Antimicrobial Peptide (LAP) in Bovine Milk, Journal of Dairy Science, 92(6), 2691-2695, 20090601
  105. Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland, ANIMAL SCIENCE JOURNAL, 80(4), 446-450, 2009
  106. Immunolocalization of lingual antimicrobial peptide (LAP) in the bovine mammary gland, ANIMAL SCIENCE JOURNAL, 80(4), 446-450, 2009
  107. Expression of avian beta-defensins in the oviduct and effects of lipopolysaccharide on their expression in the vagina of hens, POULTRY SCIENCE, 87(5), 979-984, 2008
  108. Poultry production profile and expected future projection in Bangladesh, WORLDS POULTRY SCIENCE JOURNAL, 64(1), 99-117, 2008
  109. Expression of vascular endothelial growth factor receptors in bovine cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 43(3), 267-271, 2008
  110. Changes in the localization of immunoreactive avian beta-defensin-12 in ovarian follicles during follicular growth and in response to lipopolysaccharide, JOURNAL OF POULTRY SCIENCE, 45(3), 210-214, 2008
  111. Mechanism of Prolonged Sperm Storage and Sperm Survivability in Hen Oviduct: A Review, AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY, 60(6), 477-481, 2008
  112. Expression of Avian beta-Defensin 3, an Antimicrobial Peptide, by Sperm in the Male Reproductive Organs and Oviduct in Chickens: An Immunohistochemical Study, POULTRY SCIENCE, 87(12), 2653-2659, 2008
  113. Ovarian Cyclicity and Reproductive Performance of Holstein Cows Carrying the Mutation of Complex Vertebral Malformation in Japan., Reproduction in Domestic Animals, IN PRESS, 20080101
  114. Changes in fecal progestagen profile after excretion in miniature pigs, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(5), 1107-1112, 2007
  115. Deficient proliferation and apoptosis in the granulosa and theca interna cells of the bovine cystic follicle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(5), 1119-1124, 2007
  116. Changes in the expression of gallinacins, antimicrobial peptides, in ovarian follicles during follicular growth and in response to lipopolysaccharide in laying hens (Gallus domesticus), REPRODUCTION, 133(1), 127-133, 2007
  117. Follicular cysts in dairy cows, ANIMAL SCIENCE JOURNAL, 78(1), 1-6, 2007
  118. Fecal progestagen and estrone during pregnancy in a giraffe: A case report, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(1), 159-164, 2007
  119. Changes in the expression of Toll-like receptor mRNAs during follicular growth and in response to lipopolysaccharide in the ovarian follicles of laying hens, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 53(6), 1227-1235, 2007
  120. Involvement of plasma progesterone, Oestradiol-17 beta and cortisol in ovulatory response to gonadotropin-releasing hormone in dairy cows with cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 42(4), 370-375, 2007
  121. Preparation and Application for Immunocytochemistry of Antibody to Gallinacin-3, an Antimicrobial Peptide, in Chicken., Journal of Poultry Science, 44(4), 433-438, 20071001
  122. Changes in the mRNA expressions of sperm Transforming Growth Factor beta-isoforms and their receptors with reference to fertility during in vitro storage of fowl semen., Journal of Poultry Science, 44(2), 189-197, 20070401
  123. Studies of the role of steroid hormone in the regulation of oocyte maturation in cattle, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 4, 20060203
  124. Expression of transforming growth factor-beta isoforms and their receptors in utero-vaginal junction of hen oviduct in presence or absence of resident sperm with reference to sperm storage, REPRODUCTION, 132(5), 781-790, 2006
  125. Effects of age and gonadal steroids on the localization of antigen presenting cells in the epididymis of the male chicken, gallus domesticus, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(3), 363-371, 2006
  126. Effects of age, egg-laying activity, and Salmonella-inoculation on the expressions of gallinacin mRNA in the vagina of the hen oviduct, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 52(2), 211-218, 2006
  127. Enzyme Histochemical Localization of Peroxidase Activity in the Oviduct of Immature, Laying and Non-laying Hens. (),, Journal of Poultry Science, 43(4), 401-407, 20061001
  128. Measurement of fecal progesterone concentration in miniature pig using the direct EIA method, 3, 27-29, 20060301
  129. Studies on substantially increased proteins in follicular fluid of bovine ovarian follicular cysts using 2-D PAGE and MALDI-TOF MS, REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY, 3, 20050608
  130. Expression of gap junction protein connexin 43 during follicular atresia in the ovary of swamp buffaloes, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 51(5), 675-681, 2005
  131. Microvascular distribution and vascular endothelial growth factor expression in bovine cystic follicles, DOMESTIC ANIMAL ENDOCRINOLOGY, 29(4), 634-645, 2005
  132. Enzyme immunoassay of progesterone in the feces from beef cattle to monitor the ovarian cycle, ANIMAL REPRODUCTION SCIENCE, 87(42737), 1-10, 2005
  133. Gonadotropin-induced Delta 14-reductase and Delta 7-reductase gene expression in cumulus cells during meiotic resumption of porcine oocytes, ENDOCRINOLOGY, 146(1), 186-194, 2005
  134. Pregnancy diagnosis based on the fecal progesterone concentration in beef and dairy heifers and beef cows, ANIMAL REPRODUCTION SCIENCE, 90(42798), 211-218, 2005
  135. Immunolocalization of von Willebrand factor and vascular endothelial growth factor during follicular atresia in the swamp buffalo ovary, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 51(4), 419-426, 2005
  136. Apoptosis in the antral follicles of swamp buffalo and cattle ovary: TUNEL and caspase-3 histochemistry, REPRODUCTION IN DOMESTIC ANIMALS, 40(2), 111-116, 2005
  137. Expressions of antimicrobial peptide gallinacin-1,-2 and -3 mRNAs in the oviduct of laying hens., Journal of Poultry Science, 42(4), 337-345, 20051025
  138. Direct enzyme immunoassay of fecal estrone derivatives in dairy cows. :, Animal Science Journal, 76(3), 203-207, 20050701
  139. Characteristics of cryopreserved spermatozoa from a holstein friesian bull thawed at different temperature., Journal of Internatinal Development and Cooperation, 12(1), 107-110, 20050401
  140. Pregnancy diagnosis in miniature pig by direct ELISA of oestrone derivatives in faeces, REPRODUCTION IN DOMESTIC ANIMALS, 39(1), 48-51, 2004
  141. Cell proliferation in the atretic follicles of buffalo and cattle ovary, REPRODUCTION IN DOMESTIC ANIMALS, 39(6), 405-409, 2004
  142. Determination of estrous cycle and pregnancy diagnosis in miniature pig by measuring plasma progesterone concentrations., 1, 25-28, 20040301
  143. Atresia ani with diphallus and separate scrota in a calf: a case report, THERIOGENOLOGY, 61(42924), 1205-1213, 2004
  144. Changes in the thecal vasculature during follicular atresia in the ovary of swamp buffalo, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 50(3), 315-321, 2004
  145. Incidence of silent ovulation in dairy cows during post partum period, DEUTSCHE TIERARZTLICHE WOCHENSCHRIFT, 111(1), 35-38, 2004
  146. Phosphatidylinositol 3-kinase in cumulus cells is responsible for both suppression of spontaneous maturation and induction of gonadotropin-stimulated maturation of porcine oocytes, JOURNAL OF ENDOCRINOLOGY, 179(1), 25-34, 2003
  147. Distribution of cytochrome p450-side chain cleavage in the theca interna layers of bovine small antral and cystic follicles, REPRODUCTION IN DOMESTIC ANIMALS, 38(5), 405-409, 2003
  148. Plasma concentration of estrone sulfate during pregnancy in different breeds of Japanese beef cattle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(5), 369-374, 2003
  149. Effects of body condition score in cows peripartum on the onset of postpartum ovarian cyclicity and conception rates after ovulation synchronization/fixed-time artificial insemination, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(5), 381-388, 2003
  150. Immunohistochemical localization of 3 beta-hydroxysteroid dehydrogenase in the granulosa and theca interna layers of bovine cystic follicles, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 49(3), 227-233, 2003
  151. Luteinizing hormone receptor formation in cumulus cells surrounding porcine oocytes and its role during meiotic maturation of porcine oocytes, BIOLOGY OF REPRODUCTION, 68(4), 1142-1149, 2003
  152. Effects of estrone sulfate injection on reproductive functions in male Japanese quail., Journal of Poultry Science, 40, 243-247, 20031001
  153. Direct enzyme immunoassay of progesterone in bovine plasma., Anim Sci J, 74, 369-373, 20031001
  154. Direct enzyme immunoassay of estrone sulfate in the plasma of cattle, J Reprod Dev, 48(1), 75-78, 20020201
  155. Direct enzyme immunoassay of estrone sulfate in the plasma of cattle, JOURNAL OF REPRODUCTION AND DEVELOPMENT, 48(1), 75-78, 2002
  156. Survival of boar spermatozoa frozen in diluents of varying osmolality, THERIOGENOLOGY, 56(3), 447-458, 20010801
  157. Changes in the Localization of Immunoreactive von Willebrand Factor in Microvascular Network of Bovine Ovarian Follicles during Atresia, Animal Science Journal, 72(6), 473-482, 20011101
  158. An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct, Reproduction, 122(4), 581‐586, 20011001
  159. An in situ hybridization study of the effects of artificial insemination on the localization of cells expressing MHC class II mRNA in the chicken oviduct, REPRODUCTION, 122(4), 581-586, 2001
  160. Effect of the Factor inhibiting germinal vesicle breakdown on the disruption of gap junctions and cumulus expansion of pig cumulus-oocyte complexes cultured in vitro, REPRODUCTION, 121(2), 249-257, 2001
  161. Immunocytochemical study of cell proliferation in the cystic ovarian follicles in cows, THERIOGENOLOGY, 54(7), 1159-1169, 20001015
  162. Localization of apoptotic cells in the cystic ovarian follicles of cows: A DNA-end labeling histochemical study, THERIOGENOLOGY, 53(4), 897-904, 20000301
  163. Involvement of meiotic resumption in the disruption of gap junctions between cumulus cells attached to pig oocytes., Journal of Reproduction and Fertility, 113, 167-172, 1998
  164. Cumulus cells suppress meiotic progression in pig oocytes cultured in vitro., Theriogenology, 45, 1479-1489, 1996

Invited Lecture, Oral Presentation, Poster Presentation

  1. Innate immunity of mammary gland and recent developments in treatments for mastitis, Naoki Isobe, 75th Annual Scientific Sessions 2023, 2023/09/15, With Invitation, English, 75th Annual Scientific Sessions 2023, Sri Lanka
  2. Cause of NG milk, Naoki Isobe, Japan Dairy Farming Youth Research Federation Kansai Council, 2023/07/06, With Invitation, Japanese, Japan Dairy Farming Youth Research Federation Kansai Council, Himeji Labor Hall
  3. cause of no growth milk, Naoki Isobe, NOSAI Hiroshima Technical Seminor, 2023/03/10, With Invitation, Japanese
  4. innate immunity in dairy cows, Naoki Isobe, Chiba prefecture technical seminor, 2022/12/09, With Invitation, Japanese, Shiyokai, NOSAI chiba
  5. No growth milk: cause and solution, Naoki Isobe, 7th Hiroshima University Dairy Seminor, 2022/11/16, With Invitation, Japanese, RCAS, Kurara, Higashi-Hiroshima center
  6. Antimicrobial components in mastitis, Naoki Isobe, 20220821, 2022/08/21, With Invitation, Japanese, Bovine Mastitis Rresearch, Aenoac
  7. Effect of Repeated Intrauterine Infusion of Lipopolysaccharide on Mastitis in Goat., Jirapat Jaisue, Takahiro Nii, Yukinori Yoshimura, Yusaku Tsugami, Naoki Isobe, Jirapat Jaisue, Takahiro Nii, Yukinori Yoshimura, Yusaku Tsugami, Naoki Isobe, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  8. Effects of medium-chain fatty acids supplementation on immunoglobulin level in dairy calves., Shimizu M, Sugino T, Isobe N, Shimizu M, Sugino T, Isobe N, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia,
  9. Changes in innate immune components in goat mammary gland applied with menthol., Ruri Ohno, Yusaku Tsugami, Takahiro Nii, Yukinori Yoshimura, Ken Kobayashi, Naoki Isobe, Ruri Ohno, Yusaku Tsugami, Takahiro Nii, Yukinori Yoshimura, Ken Kobayashi, Naoki Isobe, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  10. Anti-inflammatory effect of ozone infused into mammary gland of goat., Masato Hirano, Takahiro Nii, Yukinori Yoshimura, Gotaro Shiota, Naoki Isobe, Masato Hirano, Takahiro Nii, Yukinori Yoshimura, Gotaro Shiota, Naoki Isobe, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  11. Effect of oral administration of nucleotide on immunoglobulin production in goat., Kohei Murata, Takahiro Nii, Yukinori Yoshimura, Kazutoshi Ueno, Naoki Isobe, Kohei Murata, Takahiro Nii, Yukinori Yoshimura, Kazutoshi Ueno, Naoki Isobe, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  12. Effects of intra-vaginal administration of lactic acid bacteria on the oviductal mucosal barrier function of the laying hens., Taro Shinkoda, Naoki Isobe, Yukinori Yoshimura, Takahiro Nii, Taro Shinkoda, Naoki Isobe, Yukinori Yoshimura, Takahiro Nii, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  13. Effect of local heat treatment on innate immune function in goat mammary glands., Yusaku Tsugami, Yuki Ishiba, Naoki Suzuki, Takahiro Nii, Yukinori Yoshimura, Ken Kobayashi, and Naoki Isobe, Yusaku Tsugami, Yuki Ishiba, Naoki Suzuki, Takahiro Nii, Yukinori Yoshimura, Ken Kobayashi, and Naoki Isobe, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, Gadjah Mada University, Gadjah Mada University, Indonesia
  14. Effects of Partially Hydrolyzed Guar Gum Feeding on Intestinal Environment of Laying Hens., Takahiro Nii, Naoki Isobe, Yukinori Yoshimura, Takahiro Nii, Naoki Isobe, Yukinori Yoshimura, The 9th International Seminar on Tropical Animal Production (ISTAP), 2021/09/21, Without Invitation, English, University of Gadjah Mada, Indonesia
  15. Relationship between milk Ig level and inflammation status induced by intramammary infusion of E.coli, Shimizu M,Nii T,Yoshimura Y,Isobe N, Shimizu M,Nii T,Yoshimura Y,Isobe N, Animal Science Congress, 2021/09/14, Without Invitation, Japanese, Japanese Animal Science Association
  16. Lipopolysaccharides infusion into the uterus causes inflammation in the mammary gland, Fika Yuliza Purba1, Takahiro Nii, Yukinori Yoshimura, Naoki Isobe, Fika Yuliza Purba1, Takahiro Nii, Yukinori Yoshimura, Naoki Isobe, 2021/03/28, Without Invitation, Japanese
  17. Antimicrobial components for the local defense in ruminant mammary gland, ISOBE Naoki, The 3rd International Conference of Animal Science and Technology (ICAST 3), 2020/11, With Invitation, English, Hasanuddin University, Hasanuddin University
  18. Inflammatory responses in the mammary gland after intrauterine infusion of lipopolysaccharide in dexamethasone-treated goats, Purba FY, Nii T, Yoshimura Y, Isobe N, Purba FY, Nii T, Yoshimura Y, Isobe N, 1st International Veterinay Science Virtual Conference (ICVS), 2020/10/04, Without Invitation, English, Hasanuddin University, Hasanuddin University
  19. Effect of oral administration of colostrum on the antimicrobial peptides concentration in goat milk., Isobe N, Kubo K, Ueno K, Nii T, Yoshimura Y, The 8th International Seminar on Tropical Animal Production (ISTAP) , 2019/09/23, Without Invitation, English, The 8th International Seminar on Tropical Animal Production (ISTAP) , Yogyakarta, Indonesia
  20. Different concentration of antimicrobial components in bovine milk infected with different pathogens, Isobe N, Kawai K, Shinozuka Y, Purba YF, Nii T, Yoshimura Y, The 30th World Buiatrics Congress, 2018/08/29, Without Invitation, English, World Buiatrics, Sapporo Convention Center, Sapporo
  21. Effect of mastitis on the reproductive functions in pregnant goat., Isobe N, Kodama N, Nii T, Yoshimura Y, 18th AAAP Congress., 2018/08/01, Without Invitation, English, Asian Austrasian Animal Production, Sarawak, Malaysia
  22. Effect of tentative cessation of milking for 3 days on innate immune components in goat milk, Naoki Isobe, Jo Ueda, Yukinori Yoshimura, 2018 Annual Meeting of the American Dairy Science Association, 2018/07/25, Without Invitation, English, American Dairy Science Association, Knoxville,Tennessee, USA
  23. innate immune function for the prevention of mastitis, Naoki Isobe, 2017/10/06, With Invitation, Japanese
  24. mastitis and innate immunity, Naoki Isobe, 2017/05/27, With Invitation, Japanese
  25. Cessation of milking and antimicrobial peptides, Naoki Isobe, Bovine Mastitis Research, 2017/04/15, With Invitation, Japanese
  26. changes in vial bacterial count in mastitic milk and its reason, Naoki Isobe, 2017/03/07, With Invitation, Japanese
  27. Mastitis and innate immunity, Naoki Isobe, 2016/11/05, With Invitation, Japanese, Rakuno Gakuen University
  28. Effect of steroid hormone on innate immune response induced by Staphylococcus aureus in mammary gland of goat., Isobe N, Kuwahara K, Yoshimura Y, The 17th AAAP Animal Science Congress, 2016/08/22, Without Invitation, English, AAAP, Kyusyusangyo University
  29. Changes in pathogen number during preservation of milk derived from mastitic dairy cows., Isobe N, Hisaeda K, Koshiishi T, Watanabe M, Miyake H, Yoshimura Y, The 6th International Seminar on Tropical Animal Production (ISTAP), 2015/10/20, Without Invitation, English, Yogyakarta, Indonesia
  30. Innate immunity in mammary gland, Naoki Isobe, 2015/02/15, With Invitation, Japanese
  31. Association of reproductive performance with somatic cell count in milk of dairy cows., Isobe N, Iwamoto C, Yoshimura Y, The 16th AAAP Animal Science Congress, 2014/10/10, Without Invitation, English, Yogyakarta, Indonesia
  32. Antimicrobial factors in milk, Naoki Isobe, 2014/08/22, With Invitation, Japanese, NOSAI

Awards

  1. 2021/09/28, English Presentation Award, Japan Animal Science Association, Relationship between milk Ig level and inflammation status induced by intramammary infusion of E.coli
  2. 2018/03/28, The 2018 Award for Excellence in Reviewing for Animal Science Journal in 2017, 公益社団法人日本畜産学会編集委員長
  3. 2015/03/28, Animal Science Journal Reviewers Award (Reviewers賞), 日本畜産学会編集委員会

External Funds

Acceptance Results of Competitive Funds

  1. Prevention of calf diarrhea by utilizing antimicrobial peptide in colostrum, 2017, 2017
  2. KAKENHI, 2015, 2017
  3. 2015
  4. KAKENHI, 2012, 2014
  5. 2012
  6. 2011, 2013
  7. KAKENHI, Studies on the mechanism of antimicrobial peptide synthesis for a strategy to enhance the innate immunity in hen oviduct, 2009, 2012
  8. KAKENHI, Development of Fertility Improvement Program in Dairy Cows based on Postpartum Reproductive Monitoring System, 2008, 2010
  9. KAKENHI, 2006, 2008
  10. KAKENHI, 2006, 2008
  11. KAKENHI, 2004, 2005
  12. KAKENHI, Environmental pollution by sex steroids and assessment for their effects on animals and plants, 2003, 2004
  13. KAKENHI, 2000, 2000
  14. KAKENHI, Studies on potential of domestic ruminants in tropical Asia, 2000, 2003
  15. KAKENHI, Feto-Placental Function during Pregnancy and the Calf Viability, 2000, 2001

Social Activities

History as Peer Reviews of Academic Papers

  1. 2021, Animal Science Journal, Editor
  2. 2020, Bioscience, Biotechnology, and Biochemistry, Others, 1
  3. 2020, Animal Science Journal, Editor, 5
  4. 2014, Animal Science Journal, Others
  5. 2014, Journal of Reproduction and Development
  6. 2014, Domestic Animal Endocrinology
  7. 2014, BMC Veterinary
  8. 2014, Current Protein & Peptide Science
  9. Veterinary Research