KIYOMI NIGORIKAWA

Last Updated :2026/06/08

Affiliations, Positions
Graduate School of Biomedical and Health Sciences, Assistant Professor
E-mail
knigohiroshima-u.ac.jp
Self-introduction
I am investigating intracellular membrane traffic involving phosphoinositides.

Basic Information

Major Professional Backgrounds

  • 1995/10/01, 2002/03/31, Hiroshima University, Faculty of Medicine
  • 2002/04/01, 2004/03/31, Hiroshima University, Faculty of Medicine
  • 2004/04/01, 2007/03/31, Hiroshima University, Technical Center
  • 2007/04/01, 2012/03/31, Hiroshima University, Graduate School of Biomedical Sciences, Assistant Professor
  • 2012/04/01, 2019/03/31, Hiroshima University, Institute of Biomedical & Health Sciences, Assistant Professor
  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Assistant Professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Biophere Sciences, Japan

Academic Degrees

  • Doctor of Philosophy in Pharmaceutical Science, Hiroshima University
  • Master, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Pharmaceutical Sciences

In Charge of Primary Major Programs

  • Pharmaceutical Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Pharmacology in pharmacy

Research Keywords

  • membrane traffic
  • Phosphoinositides
  • CRISPR activation
  • autophagy
  • phagocytosis

Affiliated Academic Societies

  • The Pharmaceutical Society of Japan

Educational Activity

Course in Charge

  1. 2026, Undergraduate Education, 1Term, Biological Chemistry III
  2. 2026, Undergraduate Education, 2Term, Physiological Chemistry
  3. 2026, Undergraduate Education, Intensive, Physiological Chemistry
  4. 2026, Undergraduate Education, Second Semester, Experiments of Biological Chemistry
  5. 2026, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences I
  6. 2026, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences II
  7. 2026, Undergraduate Education, Second Semester, Special laboratory Works in Pharmaceutical Sciences III
  8. 2026, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy I
  9. 2026, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy II
  10. 2026, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy III
  11. 2026, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  12. 2026, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Physiological Chemistry
  13. 2026, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Physiological Chemistry
  14. 2026, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Physiological Chemistry
  15. 2026, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Physiological Chemistry
  16. 2026, Graduate Education (Master's Program) , First Semester, Seminar on Mechanisms of Signal Transduction
  17. 2026, Graduate Education (Master's Program) , Second Semester, Seminar on Mechanisms of Signal Transduction
  18. 2026, Graduate Education (Master's Program) , First Semester, Research on Mechanisms of Signal Transduction
  19. 2026, Graduate Education (Master's Program) , Second Semester, Research on Mechanisms of Signal Transduction
  20. 2026, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Physiological Chemistry for Medicinal Sciences
  21. 2026, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Physiological Chemistry for Medicinal Sciences
  22. 2026, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Physiological Chemistry for Medicinal Sciences
  23. 2026, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Physiological Chemistry for Medicinal Sciences

Research Activities

Academic Papers

  1. Screening strategy to identify Cas9 variants with higher HDR activity based on diphtheria toxin, JOURNAL OF BIOMEDICAL SCIENCE, 32(1), 20251203
    Link to Paper Search Results by DOI
  2. Cell cycle-dependent regulation of CRISPR-Cas9 repetitive activation by anti-CRISPR and Cdt1 fusion in the CRISPRa system, FEBS LETTERS, 599(6), 828-837, 202503
    Link to Paper Search Results by DOI
  3. Sulfoxide-Mediated Cys-Trp-Selective Bioconjugation that Enables Protein Labeling and Peptide Heterodimerization, Chemistry Europe, 2(3-4), e202400014, 20240608
    Link to Paper Search Results by DOI
  4. A high dose KRP203 induces cytoplasmic vacuoles associated with altered phosphoinositide segregation and endosome expansion, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 718, 20240723
    Link to Paper Search Results by DOI
  5. CRISPRa Analysis of Phosphoinositide Phosphatases Shows That TMEM55A Is a Positive Regulator of Autophagy, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 47(6), 1148-1153, 202406
  6. Discovery of Potent DAG-Lactone Derivatives as HIV Latency Reversing Agents, ACS INFECTIOUS DISEASES, 10(6), 2250-2261, 20240521
    Link to Paper Search Results by DOI
  7. SpCas9-HF1 enhances accuracy of cell cycle-dependent genome editing by increasing HDR efficiency, and by reducing off-target effects and indel rates, MOLECULAR THERAPY NUCLEIC ACIDS, 35(1), 20240312
    Link to Paper Search Results by DOI
  8. Cas9-Geminin and Cdt1-fused anti-CRISPR protein synergistically increase editing accuracy, FEBS LETTERS, 597(7), 985-994, 202304
    Link to Paper Search Results by DOI
  9. PIKfyve accelerates phagosome acidification through activation of TRPML1 while arrests aberrant vacuolation independent of the Ca2+ channel, JOURNAL OF BIOCHEMISTRY, 165(1), 75-84, 201901
    Link to Paper Search Results by DOI
  10. Lysophosphatidylinositol-acyltransferase-1 is involved in cytosolic Ca2+ oscillations in macrophages, GENES TO CELLS, 24(5), 366-376, 201905
    Link to Paper Search Results by DOI
  11. Sac1 Phosphoinositide Phosphatase Regulates Foam Cell Formation by Modulating SR-A Expression in Macrophages, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 42(6), 923-928, 201906
    Link to Paper Search Results by DOI
  12. Phosphorylation of TMEM55B by Erk/MAPK regulates lysosomal positioning, JOURNAL OF BIOCHEMISTRY, 166(2), 175-185, 201908
    Link to Paper Search Results by DOI
  13. TMEM55a localizes to macrophage phagosomes to downregulate phagocytosis, JOURNAL OF CELL SCIENCE, 131(5), 201803
    Link to Paper Search Results by DOI
  14. Inhibitory receptor FcRIIb mediates the effects of IgG on a phagosome acidification and a sequential dephosphorylation system comprising SHIPs and Inpp4a, INNATE IMMUNITY, 23(4), 401-409, 201705
    Link to Paper Search Results by DOI
  15. Phosphoinositide phosphatase Sac3 regulates the cell surface expression of scavenger receptor A and formation of lipid droplets in macrophages, EXPERIMENTAL CELL RESEARCH, 357(2), 252-259, 20170815
    Link to Paper Search Results by DOI
  16. Myeloid cell-specific inositol polyphosphate-4-phosphatase type I knockout mice impair bacteria clearance in a murine peritonitis model, INNATE IMMUNITY, 22(6), 444-451, 201608
    Link to Paper Search Results by DOI
  17. Inositol Polyphosphate-4-Phosphatase Type I Negatively Regulates Phagocytosis via Dephosphorylation of Phagosomal PtdIns (3,4)P-2, PLOS ONE, 10(11), 20151104
    Link to Paper Search Results by DOI
  18. Inpp5e increases the Rab5 association and phosphatidylinositol 3-phosphate accumulation at the phagosome through an interaction with Rab20, BIOCHEMICAL JOURNAL, 464, 365-375, 20141215
    Link to Paper Search Results by DOI
  19. Involvement of Class II Phosphoinositide 3-Kinase alpha-Isoform in Antigen-Induced Degranulation in RBL-2H3 Cells, PLOS ONE, 9(10), 20141030
    Link to Paper Search Results by DOI
  20. PIKfyve Regulates the Endosomal Localization of CpG Oligodeoxynucleotides to Elicit TLR9-Dependent Cellular Responses, PLOS ONE, 8(9), 20130909
    Link to Paper Search Results by DOI
  21. Essential roles of PIKfyve and PTEN on phagosomal phosphatidylinositol 3-phosphate dynamics, FEBS LETTERS, 586(22), 4010-4015, 20121116
    Link to Paper Search Results by DOI
  22. Class-IA Phosphoinositide 3-Kinase p110 beta Triggers GPCR-Induced Superoxide Production in p110 gamma-Deficient Murine Neutrophils, JOURNAL OF PHARMACOLOGICAL SCIENCES, 120(4), 270-279, 201212
    Link to Paper Search Results by DOI
  23. C5a controls TLR-induced IL-10 and IL-12 production independent of phosphoinositide 3-kinase, JOURNAL OF BIOCHEMISTRY, 149(3), 265-274, 201103
    Link to Paper Search Results by DOI
  24. Phosphoinositide 3-Kinasec Controls the Intracellular Localization of CpG to Limit DNA-PKcs-Dependent IL-10 Production in Macrophages, PLOS ONE, 6(10), 20111028
    Link to Paper Search Results by DOI
  25. Specific role of phosphoinositide 3-kinase p110{alpha} in the regulation of phagocytosis and pinocytosis in macrophages, Biochem J, 423(1), 99-108, 20090914
  26. Negative Regulation of Class IA Phosphoinositide 3-kinase by Protein Kinase C Limits Fc Receptor-Mediated Phagocytosis in Macrophages, JOURNAL OF BIOCHEMISTRY, 145(1), 87-94, 200901
    Link to Paper Search Results by DOI
  27. Critical roles of the p110{beta} subtype of phosphoinositide 3-kinase in lipopolysaccharide-induced Akt activation and negative regulation of nitrite production in RAW 264.7 cells, J Immunol, 180(4), 2054-2061, 20080215
  28. Role of phosphoinositide 3-kinase in innate immunity, Biol Pharm Bull, 30(9), 1617-1623, 20070901
  29. Inhibition of PTEN and activation of Akt by menadione, Biochim Biophys Acta, 1770(4), 687-693, 20070401
  30. Protein kinase C{delta} binds TIRAP/Mal to participate in TLR signaling, Mol Immunol, 44(9), 2257-2264, 20070301
  31. Protein kinase C delta binds TIRAP/Mal to participate in TLR signaling, MOLECULAR IMMUNOLOGY, 44(9), 2257-2264, 200703
    Link to Paper Search Results by DOI
  32. A naphthoquinone derivative, shikonin, has insulin-like actions by inhibiting both phosphatase and tensin homolog deleted on chromosome 10 and tyrosine phosphatases, Mol Pharmacol, 70(3), 1143-1149, 20060901
  33. Opposite effects of wortmannin and 2-(4-morpholinyl)-8-phenyl-1(4H)-benzopyran-4-one hydrochloride on toll-like receptor-mediated nitric oxide production: negative regulation of nuclear factor-{kappa}B by phosphoinositide 3-kinase., Mol Pharmacol, 69(5), 1717-1724, 20060501
  34. The effect of anionic amphiphiles on the recruitment of Rac in neutrophils, J Biochem, 136(4), 463-470, 20041001
  35. Pervanadate activates NADPH oxidase via protein kinase C-independent phosphorylation of p47-phox, Arch. Biochem. Biophys., 361(1), 1-6, 19990101
  36. A SELECTIVE GAS SENSOR USING A POLYPYRROLE THIN-FILM AS A SENSITIVE MATRIX ON A PIEZOELECTRIC CRYSTAL, JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 396(1-2), 563-567, 19951031
  37. A SELECTIVE ORGANIC VAPOR SENSOR-BASED ON SIMULTANEOUS MEASUREMENTS OF CHANGES OF MASS AND RESISTANCE OF A POLY(PYRROLE) THIN-FILM, JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 873-874, 19940407