KOJI IKEGAMI
Last Updated :2025/04/03
- Affiliations, Positions
- Graduate School of Biomedical and Health Sciences(Medicine), Professor
- E-mail
- k-ikegami
hiroshima-u.ac.jp
- Self-introduction
- At the undergraduate level, I am in charge of anatomy at the medical school. Anatomy is a "study of observation" and is the first gateway for medical students who will examine patients in the future. In lectures and practical training, I not only teach knowledge and anatomical terms, but also emphasize the importance of perspectives and views, such as "how to see things".
In research, including postgraduate education, our primary motto is "observation" as in education, and we conduct imaging-based research in which we carefully "watch" cells and various structures within cells through a microscope. In particular, we focus on primary cilia, which are single hairs that grow on the surface of cells, and the cytoskeleton, which is the "bone" inside cells that transports materials and functions during cell division. I would like to clarify how cells and those structures change in development and aging or respond to various stimuli by using live imaging and time-lapse imaging, which sometimes allow us to observe them as they are.
Basic Information
Major Professional Backgrounds
- 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor
- 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor
- 2011/08/01, 2018/03/31, Hamamatsu University School of Medicine, Associate Professor
- 2008/08/01, 2011/07/31, Hamamatsu University School of Medicine, Assistant Professor (Special Appointment)
- 2007/04/01, 2008/07/31, Mitsubishi Kagaku Institute of Life Sciences, Research associate
- 2004/04/01, 2007/03/31, Mitsubishi Kagaku Institute of Life Sciences, Postdoctoral Fellow
- 2001/04/01, 2004/03/31, JSPS, Special Researcher
Educational Backgrounds
- Hokkaido University, Graduate School of Science, Doctor Course, Division of Biological Sciences, Japan, 2001/04, 2004/03
- Hokkaido University, Graduate School of Science, Master Course, Division of Biological Sciences, Japan, 1999/04, 2001/03
- HOKKAIDO UNIVERSITY, Faculty of Science, Japan, 1995/04, 1999/03
Academic Degrees
Educational Activity
- [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
- [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
- [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
- [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine
In Charge of Primary Major Programs
Research Fields
- Medicine,dentistry, and pharmacy;Basic medicine;General anatomy (including histology / embryology)
- Biology;Biological Science;Cell biology
- Biological Sciences;Neuroscience;Neurochemistry / Neuropharmacology
Research Keywords
- cytoskeleton
- cilia and flagella
- post-translational modifications
- extracellular vesicles
Affiliated Academic Societies
- American Society for Cell Biology, 2006/06
Educational Activity
Course in Charge
- 2025, Liberal Arts Education Program1, 1Term, Foundation biology for life science
- 2025, Undergraduate Education, Second Semester, Early exposure of undergraduates to medicine
- 2025, Undergraduate Education, 2Term, Introduction for medical research
- 2025, Undergraduate Education, 4Term, Medical Neuroscience I
- 2025, Undergraduate Education, Intensive, Practice for medical research
- 2025, Undergraduate Education, 2Term, Practice of Anatomy I
- 2025, Undergraduate Education, 2Term, Practice of Anatomy I
- 2025, Undergraduate Education, Second Semester, Practice of Anatomy II
- 2025, Undergraduate Education, Second Semester, Practice of Anatomy II
- 2025, Undergraduate Education, Second Semester, Structure of human body I
- 2025, Undergraduate Education, First Semester, Structure of human body II
- 2025, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
- 2025, Graduate Education (Master's Program) , First Semester, Seminar
- 2025, Graduate Education (Master's Program) , Second Semester, Seminar
- 2025, Graduate Education (Master's Program) , First Semester, Research
- 2025, Graduate Education (Master's Program) , Second Semester, Research
- 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
- 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
- 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Research
- 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
- 2025, Graduate Education (Master's Program) , First Semester, Human Anatomy
- 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Anatomy and Developmental Biology
- 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Anatomy and Developmental Biology
- 2025, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Anatomy and Developmental Biology
- 2025, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Anatomy and Developmental Biology
Research Activities
Academic Papers
- ★, Circadian oscillation in primary cilium length by clock genes regulates fibroblast cell migration, EMBO REPORTS, 24(12), e56870, 20231206
- delta3-tubulin impairs mitotic spindle morphology and increases nuclear size in pancreatic cancer cells, MEDICAL MOLECULAR MORPHOLOGY, 57(1), 59-67, 202403
- Tubulin Polyglutamylation by TTLL1 and TTLL7 Regulate Glutamate Concentration in the Mice Brain, BIOMOLECULES, 13(5), 20230501
- Analysis of motility and mucociliary function of tracheal epithelial cilia, Methods in Cell Biology, 176, 159-180, 20230421
- Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins, Methods in Cell Biology, 175, 45-68, 20230321
- Double-layered two-directional somatopleural cell migration during chicken body wall development revealed with local fluorescent tissue labeling, Anatomical Science International, 97(4), 380-390, 202209
- ★, A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution, SCIENTIFIC REPORTS, 12(1), 20220708
- Effects of long-acting muscarinic antagonists on promoting ciliary function in airway epithelium, BMC PULMONARY MEDICINE, 22(1), 20220508
- ★, Knock-in of Labeled Proteins into 5'UTR Enables Highly Efficient Generation of Stable Cell Lines, Cell Structure and Function, 46(1), 21-35, 20210316
- Current understandings of the relationship between extracellular vesicles and cilia, The Journal of Biochemistry, 169(2), 139-145, 20210305
- Copy number variation in DRC1 is the major cause of primary ciliary dyskinesia in the Japanese population, MOLECULAR GENETICS & GENOMIC MEDICINE, 8(3), 20200301
- Influenza A virus enhances ciliary activity and mucociliary clearance via TLR3 in airway epithelium, RESPIRATORY RESEARCH, 21(1), 20201027
- Impaired mucociliary motility enhances antigen-specific nasal IgA immune responses to a cholera toxin-based nasal vaccine, INTERNATIONAL IMMUNOLOGY, 32(8), 559-568, 20200801
- Live cell imaging of dynamic behaviors of motile cilia and primary cilium, MICROSCOPY, 68(2), 99-110, 201904
- Preferential Incorporation of Administered Eicosapentaenoic Acid Into Thin-Cap Atherosclerotic Plaques, Arteriosclerosis, Thrombosis, and Vascular Biology, 39(9), 1802-1816, 201909
- An in vitro model of region-specific rib formation in chick axial skeleton: Intercellular interaction between somite and lateral plate cells, Mechanisms of Development, 159, 103568, 2019
- A targeted next-generation sequencing panel reveals novel mutations in Japanese patients with primary ciliary dyskinesia, Auris Nasus Larynx, 45(3), 585-591, 201806
- Stearate-to-palmitate ratio modulates endoplasmic reticulum stress and cell apoptosis in non-B non-C hepatoma cells, Cancer Science, 109(4), 1110-1120, 201804
- Impaired airway mucociliary function reduces antigen-specific IgA immune response to immunization with a claudin-4-targeting nasal vaccine in mice, Scientific Reports, 8(1), 2904, 201802
- Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway, SCIENTIFIC REPORTS, 8(1), 8392, 201805
- Role of caveolin-1 in hepatocellular carcinoma arising from non-alcoholic fatty liver disease, Cancer Science, 109(8), 2401-2411, 201808
- UBL3 modification influences protein sorting to small extracellular vesicles, Nature Communications, 9, 3936, 2018
- Three-dimensional tracking of microbeads attached to the tip of single isolated tracheal cilia beating under external load, Scientific Reports, 8, 15562, 201810
- ★, Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4, Molecular Biology of the Cell, 28(4), 535-544, 201702
- ★, Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease, Scientific Reports, 7, 40205, 201701
- ★, Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision, Cell, 168(1-2), 264-279, 201701
- Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating, Journal of Cell Science, 129(14), 2757-2766, 201607
- Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer, Scientific Reports, 6, 29935, 201607
- Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry, Analytical and Bioanalytical Chemistry, 407(25), 7589-7602, 201510
- Mechanical Stimulation by Postnasal Drip Evokes Cough, PloS One, 10(11), e0141823, 201511
- Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells, Biochimie, 107(A), 73-77, 201412
- Single cell lipidomics of SKBR‐3 breast cancer cells by using time‐of‐flight secondary‐ion mass spectrometry, Surface and Interface Analysis, 46(S1), 181-184, 201411
- Application of 2,5‐dihydroxyacetophenone with sublimation provides efficient ionization of lipid species by atmospheric pressure matrix‐assisted laser desorption/ionization imaging mass spectrometry, Surface and Interface Analysis, 46(12-13), 1219-1222, 201412
- Adventitial vasa vasorum arteriosclerosis in abdominal aortic aneurysm, PloS One, 8(2), e57398, 201302
- Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression, Journal of Hepatology, 59(2), 292-299, 201308
- Cytosolic carboxypeptidase 5 removes α- and γ-linked glutamates from tubulin, The Journal of Biological Chemistry, 288(42), 30445-30453, 201310
- ★, Cytosolic carboxypeptidase 1 is involved in processing α- and β-tubulin, The Journal of Biological Chemistry, 287(9), 6530-6517, 201202
- Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics, The Journal of Biological Chemistry, 287(8), 5290-5300, 201202
- CEP41 is mutated in Joubert syndrome and is required for tubulin glutamylation at the cilium, Nature Genetics, 44(2), 193-199, 201202
- ★, Ciliary and flagellar structure and function--their regulations by posttranslational modifications of axonemal tubulin, International Review of Cell and Molecular Biology, 294, 133-170, 2012
- Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid, Biophysical Journal, 101(5), 1270-1276, 201109
- SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals, PloS One, 5(7), e11755, 201007
- Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry, Medical Molecular Morphology, 43(3), 158-164, 201009
- Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray, Cancer Science, 101(1), 267-273, 201001
- ★, Unique post-translational modifications in specialized microtubule architecture, Cell Structure and Function, 35(1), 15-22, 2010
- Developments and applications of mass microscopy, Medical Molecular Morphology, 43(1), 1-5, 201003
- Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs), The Journal of Biological Chemistry, 285(30), 22936-22941, 201007
- ★, Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry, Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10490-10495, 201006
- Imaging of lipids in cultured mammalian neurons by matrix assisted laser/desorption ionization and secondary ion mass spectrometry, Surface and Interface Analysis, 42(10-11), 1606-1611, 2010
- Recombinant mammalian tubulin polyglutamylase TTLL7 performs both initiation and elongation of polyglutamylation on beta-tubulin through a random sequential pathway, Biochemistry, 48(5), 1084-1093, 200902
- ★, TTLL10 can perform tubulin glycylation when co-expressed with TTLL8, FEBS Letters, 583(12), 1957-1963, 200906
- ★, TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1, FEBS Letters, 582(7), 1129-1134, 200804
- Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors, PloS One, 3(7), e2809, 200807
- Enhancement of Trk signaling pathways by the cholestane amide conjugate MCC-257, Journal of Pharmacological Sciences, 108(1), 131-134, 200809
- ★, Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function, Proceedings of the National Academy of Sciences of the United States of America, 104(9), 3213-3218, 200702
- ★, TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites, The Journal of Biological Chemistry, 281(41), 30707-30716, 200610
- Hypo-osmotic shock induces nuclear export and proteasome-dependent decrease of UBL5, Biochemical and Biophysical Research Communications, 350(3), 610-615, 200611
- N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) suppresses neuritic degeneration caused by different experimental paradigms including in vitro Wallerian degeneration, Brain Research, 1030(1), 81-93, 200412
- ★, Non-apoptotic neurite degeneration in apoptotic neuronal death: Pivotal role of mitochondrial function in neurites, Neuroscience, 122(3), 617-626, 2003
- Membrane depolarization-mediated survival of sympathetic neurons occurs through both phosphatidylinositol 3-kinase- and CaM kinase II-dependent pathways, Brain Research, 866(1-2), 218-226, 200006
Publications such as books
- 2023/04/21, Cilia : from mechanisms to disease - part B, Academic Press, an imprint of Elsevie, 2023, 202304, Scholarly Book, Joint work, English, edited by José Manuel Bravo-San Pedro, Lorenzo Galluzzi, 9780443185885
- 2023/03/21, Cilia : from mechanisms to disease - part A, Academic Press, an imprint of Elsevie, 2023, 202303, Scholarly Book, Joint work, English, edited by José Manuel Bravo-San Pedro, Lorenzo Galluzzi, 9780443185861
- 2016, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience, Humana Press, 2016, Scholarly Book, Joint work, English, 1493934708
- 2010, Imaging mass spectrometry - protocols for mass microscopy, Springer, 2010, Scholarly Book, Joint work, English, 4431094245
Invited Lecture, Oral Presentation, Poster Presentation
- Lipoquality-mediated regulation of primary cilia dynamics, Ikegami Koji, Setou Mitsutoshi, International Symposium on Imaging Frontier 2017, 2017/07/09, Without Invitation, English, Tokyo
- Evidence for release of ciliary components into extracellular fluid, Ikegami Koji, Setou Mitsutoshi, The 28th CDB meeting, 2016/11/29, Without Invitation, English, Kobe
- Release of cilia components into extracellular space, Ikegami Koji, Ireland – Japan Joint Seminar, 2016/10/12, With Invitation, English, Tokyo
- Posttranslational polymodifications of tubulin and other Targets, Ikegami Koji, Albert Einstein College of Medicine Special Seminar, 2013/07/01, Without Invitation, English, New York
- Analysis of Polymodification-performing Enzymes with Raster Image Correlation Spectroscopy, Ikegami Koji, IMB-Olympus Microscopy Suite Opening Ceremony, Live Cell Imaging Seminer, 2011/05, With Invitation, English, IMB/Olympus, Singapore
- Tubulin polyglutamylation for structural and functional asymmetry of cilia, Ikegami Koji, Sato Shobu, Nakamura Kenji, Setou Mitsutoshi, ASCB/JSCB/RIKEN CDB Meeting, 2009/09, Without Invitation, English, ASCB, Kyoto
- Loss of tubulin polyglutamylation is associated with a lack of central apparatus in tracheacilia axoneme, dysregulated cilia beating, and severe ceaseless cough in TTLL1-deficient mouse, Ikegami Koji, Sato Shobu, Setou Mitsutoshi, 47th ASCB Annual Meeting, 2007/12, Without Invitation, English, ASCB, Washington DC