KOJI IKEGAMI

Last Updated :2024/06/04

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Professor
E-mail
k-ikegamihiroshima-u.ac.jp
Self-introduction
At the undergraduate level, I am in charge of anatomy at the medical school. Anatomy is a "study of observation" and is the first gateway for medical students who will examine patients in the future. In lectures and practical training, I not only teach knowledge and anatomical terms, but also emphasize the importance of perspectives and views, such as "how to see things".
    In research, including postgraduate education, our primary motto is "observation" as in education, and we conduct imaging-based research in which we carefully "watch" cells and various structures within cells through a microscope. In particular, we focus on primary cilia, which are single hairs that grow on the surface of cells, and the cytoskeleton, which is the "bone" inside cells that transports materials and functions during cell division. I would like to clarify how cells and those structures change in development and aging or respond to various stimuli by using live imaging and time-lapse imaging, which sometimes allow us to observe them as they are.

Basic Information

Major Professional Backgrounds

  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor
  • 2018/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor
  • 2011/08/01, 2018/03/31, Hamamatsu University School of Medicine, Associate Professor
  • 2008/08/01, 2011/07/31, Hamamatsu University School of Medicine, Assistant Professor (Special Appointment)
  • 2007/04/01, 2008/07/31, Mitsubishi Kagaku Institute of Life Sciences, Research associate
  • 2004/04/01, 2007/03/31, Mitsubishi Kagaku Institute of Life Sciences, Postdoctoral Fellow
  • 2001/04/01, 2004/03/31, JSPS, Special Researcher

Educational Backgrounds

  • Hokkaido University, Graduate School of Science, Doctor Course, Division of Biological Sciences, Japan, 2001/04, 2004/03
  • Hokkaido University, Graduate School of Science, Master Course, Division of Biological Sciences, Japan, 1999/04, 2001/03
  • HOKKAIDO UNIVERSITY, Faculty of Science, Japan, 1995/04, 1999/03

Academic Degrees

  • PhD, Hokkaido University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

In Charge of Primary Major Programs

  • Medicine
  • Life Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;General anatomy (including histology / embryology)
  • Biology;Biological Science;Cell biology
  • Biological Sciences;Neuroscience;Neurochemistry / Neuropharmacology

Research Keywords

  • cytoskeleton
  • cilia and flagella
  • post-translational modifications
  • extracellular vesicles

Affiliated Academic Societies

Educational Activity

Course in Charge

  1. 2024, Liberal Arts Education Program1, 1Term, Foundation biology for life science
  2. 2024, Undergraduate Education, 2Term, Introduction for medical research
  3. 2024, Undergraduate Education, 4Term, Medical Neuroscience I
  4. 2024, Undergraduate Education, Intensive, Practice for medical research
  5. 2024, Undergraduate Education, 2Term, Practice of Anatomy I
  6. 2024, Undergraduate Education, 2Term, Practice of Anatomy I
  7. 2024, Undergraduate Education, Second Semester, Practice of Anatomy II
  8. 2024, Undergraduate Education, Second Semester, Practice of Anatomy II
  9. 2024, Undergraduate Education, First Semester, Structure of human body
  10. 2024, Undergraduate Education, Second Semester, Structure of human body I
  11. 2024, Graduate Education (Master's Program) , First Semester, Seminar
  12. 2024, Graduate Education (Master's Program) , Second Semester, Seminar
  13. 2024, Graduate Education (Master's Program) , First Semester, Research
  14. 2024, Graduate Education (Master's Program) , Second Semester, Research
  15. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  16. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  17. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  18. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  19. 2024, Graduate Education (Master's Program) , First Semester, Human Anatomy
  20. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Anatomy and Developmental Biology
  21. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Anatomy and Developmental Biology
  22. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Anatomy and Developmental Biology
  23. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Anatomy and Developmental Biology

Research Activities

Academic Papers

  1. Tubulin Polyglutamylation by TTLL1 and TTLL7 Regulate Glutamate Concentration in the Mice Brain, Biomolecules, 13(5), 784, 20230501
  2. Analysis of motility and mucociliary function of tracheal epithelial cilia, Methods in Cell Biology, 176, 159-180, 20230421
  3. Time-lapse imaging of primary cilium behavior with physiological expression of fluorescent ciliary proteins, Methods in Cell Biology, 175, 45-68, 20230321
  4. Double-layered two-directional somatopleural cell migration during chicken body wall development revealed with local fluorescent tissue labeling, Anatomical Science International, 97(4), 380-390, 202209
  5. ★, A pair of primers facing at the double-strand break site enables to detect NHEJ-mediated indel mutations at a 1-bp resolution, SCIENTIFIC REPORTS, 12(1), 20220708
  6. Effects of long-acting muscarinic antagonists on promoting ciliary function in airway epithelium, BMC PULMONARY MEDICINE, 22(1), 20220508
  7. ★, Knock-in of Labeled Proteins into 5'UTR Enables Highly Efficient Generation of Stable Cell Lines, Cell Structure and Function, 46(1), 21-35, 20210316
  8. Current understandings of the relationship between extracellular vesicles and cilia, The Journal of Biochemistry, 169(2), 139-145, 20210305
  9. Copy number variation in DRC1 is the major cause of primary ciliary dyskinesia in the Japanese population, MOLECULAR GENETICS & GENOMIC MEDICINE, 8(3), 20200301
  10. Influenza A virus enhances ciliary activity and mucociliary clearance via TLR3 in airway epithelium, RESPIRATORY RESEARCH, 21(1), 20201027
  11. Impaired mucociliary motility enhances antigen-specific nasal IgA immune responses to a cholera toxin-based nasal vaccine, INTERNATIONAL IMMUNOLOGY, 32(8), 559-568, 20200801
  12. Live cell imaging of dynamic behaviors of motile cilia and primary cilium, MICROSCOPY, 68(2), 99-110, 201904
  13. Preferential Incorporation of Administered Eicosapentaenoic Acid Into Thin-Cap Atherosclerotic Plaques, Arteriosclerosis, Thrombosis, and Vascular Biology, 39(9), 1802-1816, 201909
  14. An in vitro model of region-specific rib formation in chick axial skeleton: Intercellular interaction between somite and lateral plate cells, Mechanisms of Development, 159, 103568, 2019
  15. A targeted next-generation sequencing panel reveals novel mutations in Japanese patients with primary ciliary dyskinesia, Auris Nasus Larynx, 45(3), 585-591, 201806
  16. Stearate-to-palmitate ratio modulates endoplasmic reticulum stress and cell apoptosis in non-B non-C hepatoma cells, Cancer Science, 109(4), 1110-1120, 201804
  17. Impaired airway mucociliary function reduces antigen-specific IgA immune response to immunization with a claudin-4-targeting nasal vaccine in mice, Scientific Reports, 8(1), 2904, 201802
  18. Environmental responsiveness of tubulin glutamylation in sensory cilia is regulated by the p38 MAPK pathway, SCIENTIFIC REPORTS, 8(1), 8392, 201805
  19. Role of caveolin-1 in hepatocellular carcinoma arising from non-alcoholic fatty liver disease, Cancer Science, 109(8), 2401-2411, 201808
  20. UBL3 modification influences protein sorting to small extracellular vesicles, Nature Communications, 9, 3936, 2018
  21. Three-dimensional tracking of microbeads attached to the tip of single isolated tracheal cilia beating under external load, Scientific Reports, 8, 15562, 201810
  22. ★, Proper cytoskeletal architecture beneath the plasma membrane of red blood cells requires Ttll4, Molecular Biology of the Cell, 28(4), 535-544, 201702
  23. ★, Increase in α-tubulin modifications in the neuronal processes of hippocampal neurons in both kainic acid-induced epileptic seizure and Alzheimer's disease, Scientific Reports, 7, 40205, 201701
  24. ★, Dynamic Remodeling of Membrane Composition Drives Cell Cycle through Primary Cilia Excision, Cell, 168(1-2), 264-279, 201701
  25. Ttll9-/- mice sperm flagella show shortening of doublet 7, reduction of doublet 5 polyglutamylation and a stall in beating, Journal of Cell Science, 129(14), 2757-2766, 201607
  26. Accumulation of arachidonic acid-containing phosphatidylinositol at the outer edge of colorectal cancer, Scientific Reports, 6, 29935, 201607
  27. Direct profiling of the phospholipid composition of adult Caenorhabditis elegans using whole-body imaging mass spectrometry, Analytical and Bioanalytical Chemistry, 407(25), 7589-7602, 201510
  28. Mechanical Stimulation by Postnasal Drip Evokes Cough, PloS One, 10(11), e0141823, 201511
  29. Single-cell time-of-flight secondary ion mass spectrometry reveals that human breast cancer stem cells have significantly lower content of palmitoleic acid compared to their counterpart non-stem cancer cells, Biochimie, 107(A), 73-77, 201412
  30. Single cell lipidomics of SKBR‐3 breast cancer cells by using time‐of‐flight secondary‐ion mass spectrometry, Surface and Interface Analysis, 46(S1), 181-184, 201411
  31. Application of 2,5‐dihydroxyacetophenone with sublimation provides efficient ionization of lipid species by atmospheric pressure matrix‐assisted laser desorption/ionization imaging mass spectrometry, Surface and Interface Analysis, 46(12-13), 1219-1222, 201412
  32. Adventitial vasa vasorum arteriosclerosis in abdominal aortic aneurysm, PloS One, 8(2), e57398, 201302
  33. Lysophosphatidylcholine acyltransferase 1 altered phospholipid composition and regulated hepatoma progression, Journal of Hepatology, 59(2), 292-299, 201308
  34. Cytosolic carboxypeptidase 5 removes α- and γ-linked glutamates from tubulin, The Journal of Biological Chemistry, 288(42), 30445-30453, 201310
  35. ★, Cytosolic carboxypeptidase 1 is involved in processing α- and β-tubulin, The Journal of Biological Chemistry, 287(9), 6530-6517, 201202
  36. Axonal gradient of arachidonic acid-containing phosphatidylcholine and its dependence on actin dynamics, The Journal of Biological Chemistry, 287(8), 5290-5300, 201202
  37. CEP41 is mutated in Joubert syndrome and is required for tubulin glutamylation at the cilium, Nature Genetics, 44(2), 193-199, 201202
  38. ★, Ciliary and flagellar structure and function--their regulations by posttranslational modifications of axonemal tubulin, International Review of Cell and Molecular Biology, 294, 133-170, 2012
  39. Submolecular-scale imaging of α-helices and C-terminal domains of tubulins by frequency modulation atomic force microscopy in liquid, Biophysical Journal, 101(5), 1270-1276, 201109
  40. SIRT1 Regulates Thyroid-Stimulating Hormone Release by Enhancing PIP5Kgamma Activity through Deacetylation of Specific Lysine Residues in Mammals, PloS One, 5(7), e11755, 201007
  41. Detection of characteristic distributions of phospholipid head groups and fatty acids on neurite surface by time-of-flight secondary ion mass spectrometry, Medical Molecular Morphology, 43(3), 158-164, 201009
  42. Imaging mass spectrometry of gastric carcinoma in formalin-fixed paraffin-embedded tissue microarray, Cancer Science, 101(1), 267-273, 201001
  43. ★, Unique post-translational modifications in specialized microtubule architecture, Cell Structure and Function, 35(1), 15-22, 2010
  44. Developments and applications of mass microscopy, Medical Molecular Morphology, 43(1), 1-5, 201003
  45. Identification of tubulin deglutamylase among Caenorhabditis elegans and mammalian cytosolic carboxypeptidases (CCPs), The Journal of Biological Chemistry, 285(30), 22936-22941, 201007
  46. ★, Tubulin polyglutamylation is essential for airway ciliary function through the regulation of beating asymmetry, Proceedings of the National Academy of Sciences of the United States of America, 107(23), 10490-10495, 201006
  47. Imaging of lipids in cultured mammalian neurons by matrix assisted laser/desorption ionization and secondary ion mass spectrometry, Surface and Interface Analysis, 42(10-11), 1606-1611, 2010
  48. Recombinant mammalian tubulin polyglutamylase TTLL7 performs both initiation and elongation of polyglutamylation on beta-tubulin through a random sequential pathway, Biochemistry, 48(5), 1084-1093, 200902
  49. ★, TTLL10 can perform tubulin glycylation when co-expressed with TTLL8, FEBS Letters, 583(12), 1957-1963, 200906
  50. ★, TTLL10 is a protein polyglycylase that can modify nucleosome assembly protein 1, FEBS Letters, 582(7), 1129-1134, 200804
  51. Transmembrane and ubiquitin-like domain-containing protein 1 (Tmub1/HOPS) facilitates surface expression of GluR2-containing AMPA receptors, PloS One, 3(7), e2809, 200807
  52. Enhancement of Trk signaling pathways by the cholestane amide conjugate MCC-257, Journal of Pharmacological Sciences, 108(1), 131-134, 200809
  53. ★, Loss of alpha-tubulin polyglutamylation in ROSA22 mice is associated with abnormal targeting of KIF1A and modulated synaptic function, Proceedings of the National Academy of Sciences of the United States of America, 104(9), 3213-3218, 200702
  54. ★, TTLL7 is a mammalian beta-tubulin polyglutamylase required for growth of MAP2-positive neurites, The Journal of Biological Chemistry, 281(41), 30707-30716, 200610
  55. Hypo-osmotic shock induces nuclear export and proteasome-dependent decrease of UBL5, Biochemical and Biophysical Research Communications, 350(3), 610-615, 200611
  56. N-alpha-p-tosyl-L-lysine chloromethyl ketone (TLCK) suppresses neuritic degeneration caused by different experimental paradigms including in vitro Wallerian degeneration, Brain Research, 1030(1), 81-93, 200412
  57. ★, Non-apoptotic neurite degeneration in apoptotic neuronal death: Pivotal role of mitochondrial function in neurites, Neuroscience, 122(3), 617-626, 2003
  58. Membrane depolarization-mediated survival of sympathetic neurons occurs through both phosphatidylinositol 3-kinase- and CaM kinase II-dependent pathways, Brain Research, 866(1-2), 218-226, 200006

Publications such as books

  1. 2016, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience, Humana Press, 2016, Scholarly Book, Joint work, English, 1493934708
  2. 2010, Imaging mass spectrometry - protocols for mass microscopy, Springer, 2010, Scholarly Book, Joint work, English, 4431094245

Invited Lecture, Oral Presentation, Poster Presentation

  1. Lipoquality-mediated regulation of primary cilia dynamics, Ikegami Koji, Setou Mitsutoshi, International Symposium on Imaging Frontier 2017, 2017/07/09, Without Invitation, English, Tokyo
  2. Evidence for release of ciliary components into extracellular fluid, Ikegami Koji, Setou Mitsutoshi, The 28th CDB meeting, 2016/11/29, Without Invitation, English, Kobe
  3. Release of cilia components into extracellular space, Ikegami Koji, Ireland – Japan Joint Seminar, 2016/10/12, With Invitation, English, Tokyo
  4. Posttranslational polymodifications of tubulin and other Targets, Ikegami Koji, Albert Einstein College of Medicine Special Seminar, 2013/07/01, Without Invitation, English, New York
  5. Analysis of Polymodification-performing Enzymes with Raster Image Correlation Spectroscopy, Ikegami Koji, IMB-Olympus Microscopy Suite Opening Ceremony, Live Cell Imaging Seminer, 2011/05, With Invitation, English, IMB/Olympus, Singapore
  6. Tubulin polyglutamylation for structural and functional asymmetry of cilia, Ikegami Koji, Sato Shobu, Nakamura Kenji, Setou Mitsutoshi, ASCB/JSCB/RIKEN CDB Meeting, 2009/09, Without Invitation, English, ASCB, Kyoto
  7. Loss of tubulin polyglutamylation is associated with a lack of central apparatus in tracheacilia axoneme, dysregulated cilia beating, and severe ceaseless cough in TTLL1-deficient mouse, Ikegami Koji, Sato Shobu, Setou Mitsutoshi, 47th ASCB Annual Meeting, 2007/12, Without Invitation, English, ASCB, Washington DC