HIDEHIKO KAWAI

Last Updated :2024/04/03

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Associate Professor
E-mail
kawaihhiroshima-u.ac.jp
Self-introduction
I am conducting the research to understand the molecular mechanisms of cellular responses induced by various types of DNA damage, that is going to provide new insights in the prevention and therapy for diseases such as cancer.

Basic Information

Major Professional Backgrounds

  • 2000/04/01, 2002/12/31, Harvard University, Postdoctoral Fellow
  • 2003/01/01, 2003/10/31, Harvard University, Research Associate
  • 2003/11/01, 2007/03/31, Hiroshima University, Research Institute for Radiation Biology and Medicine, Research Assistant
  • 2007/04/01, 2017/08/31, Hiroshima University, Research Institute for Radiation Biology and Medicine, Assistant Professor
  • 2017/09/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical & Health Sciences (School of Pharmaceutical Sciences), Associate Professor

Educational Backgrounds

  • Hiroshima University, Graduate School, Division of Medical Sciences, Japan, 1997/04, 2000/03
  • Kanazawa University, Graduate School, Division of Pharmaceutical Sciences, Japan, 1995/04, 1997/03
  • Kanazawa University, Faculty of Pharmaceutical Science, Japan, 1991/04, 1995/03

Academic Degrees

  • Doctor of Philosophy in Medical Science, Hiroshima University
  • Master of Pharmaceutical Science, Kanazawa University

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Medicinal Sciences : Medicinal Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Pharmaceutical Sciences

Research Fields

  • Environmental science;Environmental analyses and evaluation;Risk sciences of radiation and chemicals

Research Keywords

  • radiobiology
  • Carcinogenesis
  • Senescence

Affiliated Academic Societies

  • The Molecular Biology Society of Japan
  • The Japan Radiation Research Society
  • The Japanese Environmental Mutagen Society
  • The Pharmaceutical Society of Japan

Educational Activity

Course in Charge

  1. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Nucleic Acids Biochemistry
  2. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Nucleic Acids Biochemistry
  3. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Nucleic Acids Biochemistry
  4. 2024, Graduate Education (Master's Program) , First Semester, Research on Nucleic Acids Biochemistry
  5. 2024, Graduate Education (Master's Program) , Second Semester, Research on Nucleic Acids Biochemistry
  6. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Structural Biology
  7. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Structural Biology
  8. 2024, Undergraduate Education, 2Term, Bio-Analytical Science
  9. 2024, Undergraduate Education, Second Semester, Experiments in Analytical Chemistry
  10. 2024, Graduate Education (Master's Program) , First Semester, Molecular Biology and Radiological Science
  11. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Nucleic Acids Biochemistry
  12. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Nucleic Acids Biochemistry
  13. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Nucleic Acids Biochemistry

Research Activities

Academic Papers

  1. Methylmercury Decreases AMPA Receptor Subunit GluA2 Levels in Cultured Rat Cortical Neurons, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 46(2), 292-300, 202302
  2. DDX41 coordinates RNA splicing and transcriptional elongation to prevent DNA replication stress in hematopoietic cells, LEUKEMIA, 36(11), 2605-2620, 202211
    Link to Paper Search Results by DOI
  3. Correction of monomeric enhanced green fluorescent protein (mEGFP) gene by short 5'-tailed duplexes, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 134(3), 175-181, 202209
    Link to Paper Search Results by DOI
  4. Development of a versatile high-throughput mutagenesis assay with multiplexed short-read NGS using DNA-barcoded supF shuttle vector library amplified in E. coli, ELIFE, 11, 20221010
    Link to Paper Search Results by DOI
  5. ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION, RADIATION PROTECTION DOSIMETRY, 198(13-15), 1098-1103, 20220909
    Link to Paper Search Results by DOI
  6. RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance, LIFE SCIENCE ALLIANCE, 5(12), 202212
    Link to Paper Search Results by DOI
  7. Low-Dose-Rate Irradiation Suppresses the Expression of Cell Cycle-Related Genes, Resulting in Modification of Sensitivity to Anti-Cancer Drugs, CELLS, 11(3), 202202
    Link to Paper Search Results by DOI
  8. Gene correction by 5 '-tailed duplexes with short editor oligodeoxyribonucleotides, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 132(6), 552-559, 202112
    Link to Paper Search Results by DOI
  9. Single-stranded DNA versus tailed duplex in sequence conversion oflacZ alpha DNA, NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 39(9), 1245-1250, 20200901
    Link to Paper Search Results by DOI
  10. APC/C-Cdh1 is required for the termination of chromosomal passenger complex activity upon mitotic exit, JOURNAL OF CELL SCIENCE, 133(18), 20200901
    Link to Paper Search Results by DOI
  11. Preferential digestion of PCNA-ubiquitin and p53-ubiquitin linkages by USP7 to remove polyubiquitin chains from substrates, JOURNAL OF BIOLOGICAL CHEMISTRY, 294(11), 4177-4187, 20190315
    Link to Paper Search Results by DOI
  12. Spironolactone-induced XPB degradation depends on CDK7 kinase and SCFFBXL18 E3 ligase, GENES TO CELLS, 24(4), 284-296, 20190424
    Link to Paper Search Results by DOI
  13. Stepwise multipolyubiquitination of p53 by the E6AP-E6 ubiquitin ligase complex, JOURNAL OF BIOLOGICAL CHEMISTRY, 294(41), 14860-14875, 20191011
    Link to Paper Search Results by DOI
  14. Replication stress induces accumulation of FANCD2 at central region of large fragile genes., Nucleic Acids Research, 46((6)), 2932-2944, 20180406
  15. Radiation-Induced Myofibroblasts Promote Tumor Growth via Mitochondrial ROS-Activated TGF beta Signaling, MOLECULAR CANCER RESEARCH, 16(11), 1676-1686, 201811
    Link to Paper Search Results by DOI
  16. A comparison of radiation-induced mitochondrial damage between neural progenitor stem cells and differentiated cells, CELL CYCLE, 16(6), 565-573, 201703
    Link to Paper Search Results by DOI
  17. Overexpression of Rev1 promotes the development of carcinogen-induced intestinal adenomas via accumulation of point mutation and suppression of apoptosis proportionally to the Rev1 expression level, CARCINOGENESIS, 38(5), 570-578, 20170501
    Link to Paper Search Results by DOI
  18. Metabolomic screening using ESI-FT MS identifies potential radiation-responsive molecules in mouse urine, JOURNAL OF RADIATION RESEARCH, 58(3), 273-280, 20170501
    Link to Paper Search Results by DOI
  19. ATM-mediated mitochondrial damage response triggered by nuclear DNA damage in normal human lung fibroblasts, CELL CYCLE, 16(24), 2345-2354, 20171129
    Link to Paper Search Results by DOI
  20. Mitochondrial reactive oxygen species perturb AKT/cyclin D1 cell cycle signaling via oxidative inactivation of PP2A in low-dose irradiated human fibroblasts, ONCOTARGET, 7(3), 3559-3570, 20160119
  21. Hepcidin-2 in mouse urine as a candidate radiation-responsive molecule, JOURNAL OF RADIATION RESEARCH, 57(2), 142-149, 201603
    Link to Paper Search Results by DOI
  22. RAD18 Activates the G2/M Checkpoint through DNA Damage Signaling to Maintain Genome Integrity after Ionizing Radiation Exposure, PLOS ONE, 10(2), 20150212
    Link to Paper Search Results by DOI
  23. A Novel ATM/TP53/p21-Mediated Checkpoint Only Activated by Chronic gamma-Irradiation, PLOS ONE, 9(8), 20140805
    Link to Paper Search Results by DOI
  24. Selective Enhancing Effect of Early Mitotic Inhibitor 1 (Emi1) Depletion on the Sensitivity of Doxorubicin or X-ray Treatment in Human Cancer Cells, JOURNAL OF BIOLOGICAL CHEMISTRY, 288(24), 17238-17252, 20130614
    Link to Paper Search Results by DOI
  25. Aberrant microRNA Expression in Radiation-Induced Rat Mammary Cancer: The Potential Role of miR-194 Overexpression in Cancer Cell Proliferation, RADIATION RESEARCH, 179(2), 151-159, 201302
    Link to Paper Search Results by DOI
  26. Aberrant microRNA Expression in Radiation-Induced Rat Mammary Cancer: The Potential Role of miR-194 Overexpression in Cancer Cell Proliferation, RADIATION RESEARCH, 179(2), 151-159, 2013
  27. Aurora Kinase-A Inactivates DNA Damage-Induced Apoptosis and Spindle Assembly Checkpoint Response Functions of p73, CANCER CELL, 21(2), 196-211, 20120214
    Link to Paper Search Results by DOI
  28. En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs, NUCLEIC ACIDS RESEARCH, 40(20), 10394-10407, 201211
    Link to Paper Search Results by DOI
  29. a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex., Nucleic Acids Research, 20120201
  30. Asymmetric nature of two subunits of RAD18, a RING-type ubiquitin ligase E3, in the human RAD6A-RAD18 ternary complex, NUCLEIC ACIDS RESEARCH, 40(3), 1065-1076, 201202
    Link to Paper Search Results by DOI
  31. En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs, NUCLEIC ACIDS RESEARCH, 40(20), 10394-10407, 2012
  32. The p53 inhibitors MDM2/MDMX complex is required for control of p53 activity in vivo, PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 108(29), 12001-12006, 20110719
    Link to Paper Search Results by DOI
  33. RING domain mediated interaction is a requirement for MDM2's E3 ligase activity., Cancer Research, 67(13), 6020-6030, 20070701
  34. Constitutive phosphorylation of aurora-a on ser51 induces its stabilization and consequent overexpression in cancer., PLos ONE, 2(9), E944, 20070926
  35. Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer, Cancer Research, 66, 6928-6935, 20060401
  36. Radiation-induced apoptosis and dephosphorylation of acidic ribosomal protein P2, ５９(４), 370-372, 20060401
  37. C-ABL interacts with the wave2 signaling complex to induce membrane ruffling and cell spreading., J. Biol. Chem., 281(42), 31290-31297, 20061020
  38. A novel bio-dosimetry system for exposure to ionizing radiation., Nagasaki Medical Journal, 81(特集号), 318-319, 20060901
  39. Caspase-3 mediated cleavage of LyGDI in ionizing irradiated cells: Expression of the cleaved LyGDI induced cell death, Nagasaki Medical Journal, 81(特集号), 315-317, 20060901
  40. Normal abscission of midbody during cytokinesis in X-irradaited cell having chromosome bridges., Nagasaki Medical Journal, 81(特集号), 311-314, 20060901
  41. Redistribution of Aurora-B kinase during thymic apoptosis induced by ionizing radaition, Nagasaki Medical Journal, 81(特集号), 307-310, 20060901
  42. Periostin promotes invasion and anchorage-independent growth in the metastatic process of head and neck cancer, CANCER RESEARCH, 66(14), 6928-6935, 20060715
    Link to Paper Search Results by DOI
  43. Aurora-B/AIM-1 kinase activity is involved in Ras-mediated cell transformation., Oncogene, 24(55), 1122-1127, 20050401
  44. Multiple mixed lineage leukemia (MLL) fusion proteins suppress p53-mediated response to DNA damage., J. Biol. Chem., 280(26), 24315-24321, 20050701
  45. c-Abl regulates Early Growth Response Protein (EGR1) in response to oxidative stress., Oncogene, 24(55), 8085-8092, 20051208
  46. Overexpression of Aurora-A potentiates HRAS-mediated oncogenic transformation and is implicated in oral carcinogenesis, ONCOGENE, 24(6), 1122-1127, 20050203
    Link to Paper Search Results by DOI
  47. RhoGDI-2: A major regulator of cell behavior after irradiation in hematopoietic cells., Cell Dynamics Research, 3(4), 56-63, 20040401
  48. Involvement of RhoGDI-2 in radiation-induced mammalianG2-checkpoint regulation, Journal of Cell Cycle Research, 35(6), 112-120, 20040601
  49. Phosphorylation by aurora kinase A induces Mdm2-mediated destabilization and inhibition of p53, NATURE GENETICS, 36(1), 55-62, 200401
    Link to Paper Search Results by DOI
  50. Molecular basis of p53 functional inactivation by the leukemic protein MLL-ELL., Mol. Cell. Biol., 23(12), 4230-4246, 20030601
  51. Critical contribution of the MDM2 acidic domain to p53 ubiquitination., Mol. Cell. Biol., 23(14), 4939-4947, 20030701
  52. DNA damage-induced MDMX degradation is mediated by MDM2., J. Biol. Chem., 278(46), 45946-45953, 20031101
  53. Mutual dependence of MDM2 and MDMX in their functional inactivation of p53., J. Biol. Chem., 277(22), 19251-19254, 20020501
  54. Inactivation of the NF-kappaB-dependent cell survival, a novel mechanism for the proapoptotic function of c-Abl., Mol. Cell. Biol., 22(17), 6079-6088, 20020901
  55. Stimulation of p300-mediated transcription by the kinase MEKK1., J. Biol. Chem., 276(19), 16310-16317, 20010501
  56. Mechanism of functional inactivation of a Li-Fraumeni syndrome p53 that has a mutation outside of the DNA-binding domain., Cancer Res., 61(4), 1741-1746, 20010201
  57. Subcellular distribution of p53 and p73 are differentially regulated by MDM2., Cancer Res., 61(18), 6703-6707, 20010901
  58. Dual role of p300 in the regulation of p53 stability., J. Biol. Chem., 276(49), 45928-45932, 20011201
  59. Functional interaction between p53 and NF-kB during X-ray-induced apoptosis in mouse thymocytes, 53(3), 205-208, 20000301
  60. Activation of cycline dependent kinase 2 in X-irradiated mouse thymic lymphoma cells, 75, 291-293, 20001201
  61. Molecular cloning of mouse thioredoxin reductases., Gene, 242(1-2), 321-330, 20000101
  62. Molecular cloning of mouse thioredoxin reductases, GENE, 242(1-2), 321-330, 20000125
    Link to Paper Search Results by DOI
  63. Down-regulation of nuclear factor kappaB is required for p53-dependent apoptosis in X-ray-irradiated mouse lymphoma cells and thymocytes., Cancer Res., 59(24), 6038-6041, 19991201
  64. Multiple signaling pathways controling radiation-induced apoptosis, 51(3), 284-286, 19980301
  65. Isolation and characterization of apoptosis-resistant mutants from a radiosensitive mouse lymphoma cell line., Radiat. Res., 149(1), 41-51, 19980101

Invited Lecture, Oral Presentation, Poster Presentation

  1. Comprehensive analyses of cellular responses to chronic -irradiation (II), Kawai, H., Cao, L., Shimizu, N., Sasatani, M., Iizuka, D., Matsui, H., Kanai, A., Inaba, T., Kamiya, K., Suzuki, F, The 5th International Symposium of RIRBM, 2015/03, Without Invitation, English
  2. Analysis of radiation induced mutations using next generation sequencer., Kanai, A., Nagamachi, A., Kawai, H., Matsui, H., Kamiya, K., Inaba, T, The 5th International Symposium of RIRBM, Hiroshima, 2015/03, Without Invitation, English
  3. Effects of low dose gamma irradiation on primary human endothelial cells, Zaharieva, E., Kawai, H., Inaba, T., Matusura, S., Kamiya, K., The 5th International Symposium of RIRBM, 2015/03, Without Invitation, English
  4. The effects of low dose radiation exposure in the first hours after fertilization on the development of early mouse embryos., Shimizu, N., Kawai, H., Sasatani, M., Endoh, M., Inaba, T., Kamiya, K., The 5th International Symposium of RIRBM, Without Invitation, English
  5. DNA Damage Response in Human Induced Pluripotent Stem Cells., Shimamoto, A., Kagawa, H., Kawai, H., Sakuma, T., Yamamoto, T., Shiotani B., Tahara, H., The 5th International Symposium of RIRBM, 2015/03, Without Invitation, English
  6. Perturbation of AKT/cyclin D1 signaling pathway by oxidative stresses following low dose long-term fractionated radiation in normal human fibroblasts., Shimura, T., Sasatani, M., Kamiya, K., Kawai, H., Kunugita, N., The 5th International Symposium of RIRBM, 2015/03, Without Invitation, English
  7. Dose rate-dependent cellular senescence in response to chronic -irradiation via the ATM/TP53/p21 pathway., Cao, L., Kawai, H., Sasatani, M., Iizuka, D., Inaba, T., Kamiya, K., Suzuki, F, The 5th International Symposium of RIRBM, 2015/03, Without Invitation, English
  8. Differential Response to Acute and Chronic Low Dose Gamma Irradiation in Primary Human Endothelial Cells, Elena K Zaharieva, Hidehiko Kawai, Toshiya Inaba, Shinya Matsuura, Kenji Kamiya, 15th International Congress of Radiation Research, 2015/05/26, Without Invitation, English
  9. An Integrated Approach Using High-throughput Sequencing and Imaging Technologies to the Study of Cellular Responses to Low Dose Radiation, Hidehiko Kawai, Lili Cao, Natsumi Shimizu, Hirotaka Matsui, Akinori Kanai, Tokuhisa Hirouchi, Toshiya Inaba, Kenji Kamiya, Fumio Suzuki, 15th International Congress of Radiation Research, 2015/05/26, Without Invitation, English

Awards

  1. 2015/05, Excellent Poster Award, ICRR 2015, An Integrated Approach Using High-throughput Sequencing and Imaging Technologies to the Study of Cellular Responses to Low Dose Radiation