Akio Kuroda

Last Updated :2022/01/05

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
Web Site
E-mail
akurodahiroshima-u.ac.jp
Other Contact Details
1-3-1 Kagamiyama, Higashi-Hiroshima, Hiroshima 739-8530, Japan
TEL : (+81)82-424-7758 FAX : (+81)82-424-7047

Basic Information

Major Professional Backgrounds

  • 1998/01/01, 2005/03/31, Hiroshima University, Graduate school of advanced sciences of Matter, Associated professor
  • 1997/04/01, 1998/01/01, Hiroshima University, Faculty of engineering, Associated professor
  • 1994/04/01, 1997/03/31, Hiroshima University, Faculty of engineering, Assistant professor
  • 2005/04/01, 2019/03/31, Hiroshima University, Graduate school of advanced sciences of Matter, Professor
  • 1988/04/01, 1994/03/31, Shinshu University, Faculty of textile science and technology, Assistant professor

Educational Backgrounds

  • Osaka University, Graduate School, Division of Engineering, Fermentation technology, Japan, 1986/04, 1988/03
  • Osaka University, Faculty of Engineering, Fermentation technology, Japan, 1982/04, 1986/03

Academic Degrees

  • Doctor of Engineering, Osaka University
  • Master of Engineering, Osaka University

Research Fields

  • Engineering;Process / Chemical engineering;Biofunction / Bioprocess
  • Environmental science;Environmental conservation;Environmental conscious materials and recycle
  • Environmental science;Environmental conservation;Environmental risk control and evaluation
  • Agricultural sciences;Agricultural chemistry;Applied microbiology

Research Keywords

  • protein engineering
  • silicon
  • asbestos
  • polyphosphate
  • phosphite
  • Luciferase
  • biosensing

Affiliated Academic Societies

  • The society for biotechnology, Japan
  • Phosphorus recycling promotion council
  • American society for microbiology

Educational Activity

Course in Charge

  1. 2021, Undergraduate Education, 2Term, Introduction to Fundamental Industry
  2. 2021, Undergraduate Education, 3Term, Biotechnology
  3. 2021, Undergraduate Education, Second Semester, Group Discussion of Current Biotechnology Topics
  4. 2021, Graduate Education (Master's Program) , 2Term, Lecture on Developing Communication Skills
  5. 2021, Graduate Education (Master's Program) , 2Term, Lecture on Developing Communication Skill
  6. 2021, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  7. 2021, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  8. 2021, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  9. 2021, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  10. 2021, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  11. 2021, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  12. 2021, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. Inhibition of quorum sensing in Pseudomonas aeruginosa by N-acyl cyclopentylamides, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(10), 3183-3188, 200705
  2. Oriented immobilization of antibodies on a silicon wafer using Si-tagged protein A, ANALYTICAL BIOCHEMISTRY, 385(1), 132-137, 20090201
  3. Reciprocating-flow ATP amplification system for increasing the number of amplification cycles, ANALYTICAL BIOCHEMISTRY, 395(2), 161-165, 20091215
  4. ★, Selective Detection of Airborne Asbestos Fibers Using Protein-Based Fluorescent Probes, ENVIRONMENTAL SCIENCE & TECHNOLOGY, 44(2), 755-759, 20100115
  5. Continuous-Flow ATP amplification system on a chip, SENSORS AND ACTUATORS B-CHEMICAL, 142(1), 118-122, 20091012
  6. ★, Endotoxin assay by bioluminescence using mutant firefly luciferase, ANALYTICAL BIOCHEMISTRY, 397(2), 152-155, 20100215
  7. ★, Single-step affinity purification of recombinant proteins using the silica-binding Si-tag as a fusion partner, PROTEIN EXPRESSION AND PURIFICATION, 71(1), 91-95, 201005
  8. Selective Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators, JAPANESE JOURNAL OF APPLIED PHYSICS, 49(4), 2010
  9. Electron Microscopic Analysis of Heat-Induced Leakage of Polyphosphate from a phoU Mutant of Escherichia coli, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 74(4), 865-868, 201004
  10. Bacterial phosphate metabolism and its application to phosphorus recovery and industrial bioprocesses, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 109(5), 423-432, 201005
  11. Production of 2-deoxyribose 5-phosphate from fructose to demonstrate a potential of artificial bio-synthetic pathway using thermophilic enzymes, JOURNAL OF BIOTECHNOLOGY, 148(4), 204-207, 20100802
  12. The silica-binding Si-tag functions as an affinity tag even under denaturing conditions, PROTEIN EXPRESSION AND PURIFICATION, 77(2), 173-177, 201106
  13. Detection of Antigen-Antibody Reaction Using Si Ring Optical Resonators Functionalized with an Immobilized Antibody-Binding Protein, JAPANESE JOURNAL OF APPLIED PHYSICS, 50(4), 201104
  14. Sensitivity Improvement of Biosensors Using Si Ring Optical Resonators, JAPANESE JOURNAL OF APPLIED PHYSICS, 50(4), 201104
  15. Overproduction of YjbB reduces the level of polyphosphate in Escherichia coli: a hypothetical role of YjbB in phosphate export and polyphosphate accumulation, FEMS MICROBIOLOGY LETTERS, 320(1), 25-32, 201107
  16. Why does the silica-binding protein Si-tag" bind strongly to silica surfaces? Implications of conformational adaptation of the intrinsically disordered polypeptide to solid surfaces", COLLOIDS AND SURFACES B-BIOINTERFACES, 86(2), 359-363, 20110901
  17. Feasibility of thermophilic adenosine triphosphate-regeneration system using Thermus thermophilus polyphosphate kinase, PROCESS BIOCHEMISTRY, 46(9), 1747-1752, 201109
  18. Application of an enteric coat increases the resistance to gastric juices for swallowable biosensors, SENSORS AND ACTUATORS B-CHEMICAL, 160(1), 379-383, 20111215
  19. Evaluation of Sensitivity of Fluorescence-Based Asbestos Detection by Correlative Microscopy, JOURNAL OF FLUORESCENCE, 22(1), 357-363, 201201
  20. ★, Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp strain 4506, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(4), 445-450, 201204
  21. Construction of membrane-anchoring fusion protein of Thermococcus kodakaraensis glycerol kinase and its application to repetitive batchwise reactions, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 113(4), 521-525, 201204
  22. Synthetic metabolic engineering-a novel, simple technology for designing a chimeric metabolic pathway, MICROBIAL CELL FACTORIES, 11, 20120906
  23. Cloning and characterization of chemotaxis genes in Pseudomonas aeruginosa, Bioscience= Biotechnology and Biochemistry, 63(1), 155-161, 19990401
  24. Cloning and characterization of Pseudomonas putida genes encoding the phosphate-specific transport system, Journal of Bioscience and Bioengineering, 87(3), 273-279, 19990401
  25. Regulation of bacterial phosphate Taxis and polyphosphate accumulation in pesponse to phosphate starvation stress, Journal of Bioscience, 23, 491-499, 19980401
  26. Isolation and characterization of two cryptic plasmids in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, JOURNAL OF BACTERIOLOGY, 181, 3375-3381, 19990401
  27. Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewates, Progress in Molecular and Subcellular biology, 23, 299-311, 19990401
  28. Software in microorganizms., 52(9), 11-16, 19940401
  29. Cloning of the Pseudomonas awruginosa gene encoding CDP-diglyceride synthetase, Gene, 172(1), 165-166, 19960401
  30. Molecular analysis of the phosphate sensing in Pseudomonas aeruginosa, Beijerinck Centennial Microbial physiology and gene regulation. Delf University Press, 181-182, 19950401
  31. Genetic engineering of polyphosphate accumulation in bacteria, Beijerinck Centennial Microbial physiology and gene regulation. Delf University Press, 233-234, 19950401
  32. Isolation and characterization of Enterobacter cloacae mutants which are defective in chemotaxis toward in organic phosphate, Journal of Bacriology, 179(19), 6192, 19970401
  33. Molecular Breeding of Microbes and Environmental Biotechnology, Microbes and Environmants, 13(2), 95, 19980401
  34. Molecular analysis of the phosphate-specific transport(pst)operon of Pseudomonas aeruginosa, Molecular and General Genetics, 250(6), 692-698, 19960401
  35. Cloning, sequencing and genetic mapping of a Bacillus subtilis cell wall hydrolase gene, J. Gen. Microbiol.=, 136, 2209-2216, 19900401
  36. Nucleotide sequences of the Bacillus subtilis flaD locus and a B. licheniformis homologue affecting the autolysin level and flagellation, Journal of General Microbiology(U. K. )=, 136, 1223-1230, 19900401
  37. Cloning of the Pseudomonas aeruginosa gene encoding CDP-diglyceride synthetase, Gene, 172, 165-166, 19960401
  38. Bacterial phosphonate degradation, phosphite oxidation and polyphosphate accumulation, Resources= Conservation and Recycling, 18, 125-134, 19960401
  39. Chemotactic signal transduction network in Pseudomonas aeruginosa, American Society for Microbiology, 188-194, 19960401
  40. Genetic identification of chemotactic transducers for amino acids in Pseudomonas aeruginosa, Microbiology, 143, 3223-3229, 19970401
  41. Development of a genetic system for an algal-lytic bacterium, Appl. Environ. Microbiol. =, 64, 2061-2064, 19980401
  42. Enhanced utilization of phosphonate and phosphite by Klebsiella aerogenes, Appl. Environ. Microbiol. =, 64, 3754-3758, 19980401
  43. The synthesis of optically pure enantiomers of N- acyl homoserine lactone autoinducers and their analogues, Chem. Lett., 314-315, 20010201
  44. Isolation and characterization of the Enterobacter cloacae cheR mutant defective in phosphate taxis, Biosci. Biotechnol. Biochem., 65(2), 456-458, 20010201
  45. Role of inorganic polyphosphate in promoting ribosomal protein degradation by the Lon protease in E. coli, Science, 293, 705-708, 20010701
  46. Development of rapid toxicity assays by using motile bacteria labeled with the green fluorescent protein (GFP) as a biosensor, Proceedings of 5th International Symposium on Environmental Biotechmology, 634-637, 20010701
  47. Release of polyphosphate granules from activated sludge by heat treatment and its application for phosphorus reuse and recycling, Proceedings of 5th International Symposium on Environmental Biotechmology, 23-26, 20010701
  48. Quorum sensing in gram-negative bacteria using synthetic autoinducers and thei analogues, Proceedings of 5th International Symposium on Environmental Biotechmology, 1037-1040, 20010701
  49. Polyphosphate overplus in Klebsiella aerogenes, Proceedings of 5th International Symposium on Environmental Biotechmology, 55-58, 20010701
  50. Purification and characterization of an algicidal extracellular protease of the marine bacterium Pseudoalteromonas sp. strain A28, Proceedings of 5th International Symposium on Environmental Biotechmology, 59-62, 20010701
  51. Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, J. Bacteriol., 182(3), 825-828, 20000201
  52. Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS, J. Biosci. Bioeng., 90(6), 688-690, 20000601
  53. Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa, J. Bacteriol., 182(12), 3400-3404, 20000601
  54. Development of a genetic transformation system for an alga-lysing bacterium, Appl. Environ. Microbiol. =, 64(6), 2061-2064, 19980601
  55. Physical Map Location of the Multicopy Genes Coding for Ammonia Monooxygenase and Hydroxylamine Oxidoreductase in the Ammonia-Oxidizing Bacterium Nitrosomonas sp.Strain ENI-11, J. Bacteriol., 182, 825-828, 20000401
  56. Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp. strain ENI-11, Biosc. Biochem. Biotechnol., 64, 1754-1757, 20000401
  57. Involvement of an extracellular protease in the algicidal activity of the marine bacterium Pseudoalteromonas sp. strain A28, Appl. Environ. Microbiol. =, 66, 4334-4339, 20000401
  58. Molecular analysis of polyphosphate accumulation in bacteria, Biochemistry (Moscow), 65, 362-367, 20000401
  59. The optical resolution of synthetic N-acyl-homoserine lactone autoinducers and their analogues, Chem. Lett., 314-315, 20010401
  60. Inorganic polyphosphate: a molecular tag to identify ribosomal proteins for degradation by Lon protease in E. coli, Science, 293, 705-708, 20010401
  61. ★, A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling, Biotechnol. Bioeng., 78, 333-338, 20010401
  62. A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP generation and bioluminescence reactions, Biochemical Engineering Journal, 9, 193-197, 20010401
  63. Chemotaxis of the Nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride, J. Biosci. Bioeng., 91, 322-324, 20010401
  64. Development of rapid toxicity assays by using motile bacteria labeled with the green fluorescent protein (GFP) as a biosensor., Proceedings of 5th International Symposium on Environmental Biotechmology, 634-637, 20010701
  65. Release of polyphosphate granules from activated sludge by heat treatment and its application for phosphorus reuse and recycling., Proceedings of 5th International Symposium on Environmental Biotechmology, 23-26, 20010701
  66. Quorum sensing in gram-negative bacteria using synthetic autoinducers and thei analogues., Proceedings of 5th International Symposium on Environmental Biotechmology, 1037-1040, 20010701
  67. Polyphosphate overplus in Klebsiella aerogenes, Proceedings of 5th International Symposium on Environmental Biotechmology, 55-58, 20010701
  68. Purification and characterization of polyphosphate-dependent glucokinase of Microlunatus phosphovorus, Proceedings of 5th International Symposium on Environmental Biotechmology, 1061-1064, 20010701
  69. Molecular analysis of the nitrification henes in Nitrosomonas sp. strain ENI-11, Proceedings of 5th International Symposium on Environmental Biotechmology, 67-70, 20010701
  70. Purification and characterization of an algicidal extracellular protease of the marine bacterium Pseudoalteromonas sp. strain A28, Proceedings of 5th International Symposium on Environmental Biotechmology, 59-62, 20010701
  71. Bacterial sensing system as a potential tool for rapid toxicity assays, Core Research for Environmental Science and Technology International Aquatic Environment Workshop, 103-106, 20000201
  72. Inorganic polyphosphate kinase is required to stimulate protein degradation and for adaptation to amino acid starvation in Escherichia coli, Proc. Natl. Acad. Sci. USA, 96, 14264-14269, 19990401
  73. Polyphosphate kinase as a nucleotide diphosphate kinase in Escherichia coli and Pseudomonase aeruginosa, Proc. Natl. Acad. Sci. USA, 94, 439-442, 19970401
  74. Guanosine tetra- and pentaphosphate promote accumulation of inorganic polyphosphate in Escherichia coli, J. Biol. Chem., 272, 21240-21243, 19970401
  75. Molecular cloning of a CDP-diacylglycerol synthase gene of Pseudomonas aeruginosa, Gene, 172, 165-166, 19960401
  76. Molecular cloning and characterization of a chemotactic transducer gene in Pseudomonas aeruginosa, J. Bacteriol., 177, 7019-7025, 19950401
  77. Nucleotide sequence and regulation of a new putative cell wall hydrolase gene, cwlD, which affects germination in Bacillus subtilis, J. Bacteriol., 177, 5582-5589, 19950401
  78. Effect of degS-degU mutations on the expression of sigD, encoding an alternative sigma factor, and autoysin operon of Bacillus subtilis, J. Bacteriol., 176, 5177-5180, 19940401
  79. Molecular cloning of a sporulation-specific cell wall hydrolase gene of Bacillus subtilis, J. Bacteriol., 175, 6260-6268, 19930401
  80. Molecular cloning, sequence analysis, and characterization of a new cell wall hydrolase, CwlL, of Bacillus licheniformis, Mol. Gen. Genet., 241, 380-388, 19930401
  81. Bacillus subtilis mutant deficient in the major autolytic amidase and glucosaminidase is impaired in motility, FEMS Microbiol. Letters, 112, 135-140, 19930401
  82. High-level transcription of the major Bacillus subtilis autolysin operon depends on expression of the sigma D gene and is affected by a sin (flaD) mutation, J. Bacteriol., 175, 795-801, 19930401
  83. Characterization of the Bacillus subtilis CwbA protein which stimulates cell wall lytic amidases, FEMS Microbiol. Letters, 95, 109-114, 19920401
  84. Molecular cloning and sequencing of the upstream region of the major Bacillus subtilis autolysin gene: a modifier protein exhibiting sequence homology to the major autolysin and the spoIID product, J. Gen. Microbiol.=, 138, 1067-1076, 19920401
  85. Genetic structure, isolation and characterization of a Bacillus licheniformis cell wall hydrolase, Mol. Gen. Genet., 234, 129-137, 19920401
  86. Molecular cloning and sequencing of a major Bacillus subtilis autolysin gene, J. Bacteriol., 173, 7304-7312, 19910401
  87. Purification and characterization of a cell wall hydrolase encoded by the cwlA gene of Bacillus subtilis, FEMS Microbiol. Letters, 81, 9-14, 19910401
  88. Precipitation Behavior of 1,1,di(p-Hydroxyphenyl)-Cyclohexane Clathrate Crystals From Acetone Solutions Containing d-Limonene, J. Inclusion Phenomena, 10, 305-312, 19910401
  89. An Approach to Molecular Artificial Life: bacterial intelligent behavior and its computer model, Artificial Life V (C. G. Langton and K. Shimohara= Ed.), 395-401, 19960401
  90. Behavioral responses of the ciliated protozoan Paramecium caudatum to 2,4-dichlorophenoxyacetic acid and its analogues, J. Biosci. Bioeng., 93, 416-420, 20020401
  91. Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp. sain PCC6803, Appl. Environ. Microbiol., 68, 4107-4110, 20020401
  92. Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms, Jouranal of Biotechnology, 101(1), 11-18, 20030101
  93. A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, Journal of Bioscience and Bioengineering, 95(6), 637-640, 20030601
  94. Pilot plant tests on the novel process for phosphorus recovery from municipal wastewater, Journal of Chemical Engineering of Japan, 36(10), 1143-1146, 20031001
  95. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli, J. Biol. Chem., 279, 34406-34410, 20040401
  96. ★, ATP amplification for ultrasensitive bioluminescence assay: detection of a single bacterial cell, Biosci. Biotech. Biochem., 68, 1216-1220, 20040401
  97. Mutants of the nematode Caenorhabditis elegans that are defective specifically in their attraction to cycloheximide, J. Biosci. Bioeng., 96(2), 149-153, 20030801
  98. Strictly polyphosphate-dependent glucokinase in a polyphosphate accumulating bacterium, Microlunatus phosphovorus, J. Bacteriol., 185(18), 5654-5656, 20030401
  99. Chemotaxis proteins and transducers for aerotaxis in Psudomonas aeruginosa., FEMS Microbiol. Letters, 231, 247-252, 20040401
  100. The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa, J. Biosci. Bioeng., 97(3), 184-190, 20040301
  101. Characterization of pEC01, a novel plasmid required for phosphate taxis in Enterobacter cloacae IFO 3320, Microbs Environ., 19(1), 45-52, 20040401
  102. A laboratory-scale test of anaerobic digestion and methane production after phosphorus recovery from waste activated sludge, J. Biosci. Bioeng., 97(6), 365-368, 20040601
  103. Chemotaxis proteins and transducers fro aerotaxis in Pseudomonas putida, FEMS Microbiol. Lett., 231, 247-252, 20040401
  104. Expression of Pseudomonas aeruginosa aer-2, one of two aerotaxis transducer genes, is controlled by RpoS, J. Bacteriol. (in press), 20050401
  105. Chemotaxis as sensing system for environmental pollutions, The 10th APCChE Congress proceedings, 2J-05, 20040401
  106. Biotransformation of toluene to o-cresol in a two-phase system by a solvent-tolerant bacterium, The 10th APCChE Congress proceedings, 3L-11, 20040401
  107. Role of the maxillary palpus in the feeding preference behabior of the silk worm, Bombyx mori, The 10th APCChE Congress proceedings, 3P-01-050, 20040401
  108. Control of quorum sensing systems in Pseudomonas aeruginosa by acyl homoserine lactone analogues, The 10th APCChE Congress proceedings, 3P-01-053, 20040401
  109. Characterization of Rhodococcus opacus B-4, a strain able to grow with benzene in a two-pase (organic-aqueous) medium, The 10th APCChE Congress proceedings, 3P-01-084, 20040401
  110. Improvement of phosphate recovery from wastewater by heatphos process, P1-018(ENV), 20050501
  111. Sommelier mice: Biosensor for odor discrimination among liquors, P3-035(CUR), 20050501
  112. Analysis of the activities in the medaka fish olfactory bulb by optical imaging, AROMA RESEARCH, 23(6), 276-282, 20050801
  113. Learning experience enables mice to discrimiinate odors among liquors, FFI Journal, 210(8), 765-769, 20050801
  114. Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp. strain A28, Bioscicence Biotechnology and Biochemistry, 66, 1366-1369, 20020601
  115. The effects of cyclodextrins on autoinducer activities of quorum sensing in Pseudomonas aeruginosa, Journal of Inclusion Phenomena and Macrocyclic Chemistry, 44, 381-382, 20021201
  116. Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase / oxygenase large and small subunits in Nitrosomonas sp. strain ENI-11, Bioscicence Biotechnology and Biochemistry, 66, 632-635, 20020301
  117. Development of novel biological process for recovering phosphorus from wastewater, Journal of Chemical Industry and Engineering (China), 53, SUPPL 21-22, 20021101
  118. Development of a genetic transformation system for Benzene-tolerant Rhodococcis opacus strains, Journal of Bioscience and Bioengineering, 99, 408-414, 20050425
  119. Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system, Journal of Industrial Microbiology and Biotechnology, 32, 542-547, 20051201
  120. Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitorosomonas sp. Strain ENI-11, Bioscicence Biotechnology and Biochemistry, 70(8), 1875-1881, 20060801
  121. Identification and chracterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene, The Journal of Bacteriology, 188(18), 6700-6702, 20060901
  122. Inorganic polyphosphate stimulates Lon-mediated proteolysis of nucleoid proteins in Escherichia coli, Cellular and Molecular Biology, 52(4), 23-29, 20061231
  123. Polyamines affect polyphosphate accumulation in Escherichia coli, Journa of Environmental Biotechnology, 6, 41-46, 20060601
  124. Polyphosphate-ATP amplification technology: Principle and its application to specific bacteria detection, Journa of Environmental Biotechnology, 6, 105-108, 20060601
  125. ★, The Si-tag for immobilizing proteins on a silica surface, Biotechnology and Bioengineering, 96, 1023-1029, 20070415
  126. ★, Increase in bioluminescence intensity of firefly luciferase using genetic modification, Analytical Biochemistry, 366, 131-136, 20070701
  127. Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection, J. Bioscience and Bioengineering, 104, 55-61, 20070725
  128. Effect of mineral elements on phosphorus release from heated sewage sludge, Bioresource Technology, 98, 2533-2537, 20070901
  129. ★, Use of an Escherichia coli recombinant producing thermostable polyphoaphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate, Applied and Environmental Microbiology, 73(17), 5676-5678, 20070901
  130. Transcriptional Analysis of the Multicopy hao Gene Coding for Hydroxylamine Oxidoreductase in Nitrosomonas sp. Strain ENI-11, Biosci. Biotechnol. Biochem., 1875-1881, 20070823
  131. Identification of Chemosensory Proteins for Trichloroethylene in Pseudomonas aeruginosa, Journal of bioscience and bioengeering, 99(4), 396-402, 20050425
  132. Isolation and Characterization of Benzene-Tolerant Rhodococcus opacus stains, Journal of Bioscience and Bioengineering, 99(4), 378-382, 20050425
  133. Ethylene Chemotaxis in Pseudomonas aeruginosa and Other Pseudomoonas Species, Microbes and Environments, 22(2), 186-189, 20070601
  134. Bioproduction of 3-Methlcatechol from Toluene in a Two-Phase(organic-Aqueous)System by a Genetically Modified solvent-Tolerant Pseudomonas putida Strain T-57, Journal of Environmental Biotechnology, 7(1), 39-44, 20070601
  135. ★, The Si-tag for Immobilizing Proteins on a Silicon Device, Technical Proceedings of the 2007 Nanotechnology Conference and Trade Show, 4, 571-574, 20070301
  136. Protein engineering of penicillinase as affinity ligands for bioprocessing, Journal of Fermentation and Bioengineering, 67, 315-320, 19890101
  137. Precipitation Behavior of 1,1,di(p-Hydroxyphenyl)-Cyclohexane Clathrate Crystals From Acetone Solutions Containing d-Limonene, J. Inclusion Phenomena, 10, 305-312, 19910401
  138. The role of attention in mice olfactory response, Proceedings of Asia-Pacific Biochemical Engineering Conference 2007, P1-41, 20071105
  139. Development of genome engineering technology for solvent-tolerant Rhodococcus opacus strain B4, Proceedings of Asia-Pacific Biochemical Engineering Conference 2007, P3-10, 20071107
  140. High Sensitivity Detection of ATP using Bioluminescence at an Optical Fiber End, Trans. IEEE of Japan, 10(127), 1498-1503, 20071001
  141. Bioluminescent Reaction by Immobilized Luciferase, IEEJ Trans. EIS, 131(1), 23-28, 20110101
  142. Detection of living Salmonella cells using bioluminescence, Biotechnology Letters., 31(5), 737-741, 20090501
  143. High-sensitivity detection of ATP using bioluminescence at an optical fiber end, Electronics and communications in Japan, 92(9), 1498-1503, 20091001
  144. Slot ring biosensors using silicon nitride waveguides with small temperature coefficient, Proc. SPIE, 8431(-), 84311S, 20120601
  145. Physical map location of the multicopy genes coding for ammonia monooxygenase and hydroxylamine oxidoreductase in the ammonia-oxidizing bacterium Nitrosomonas sp strain ENI-11, JOURNAL OF BACTERIOLOGY, 182(3), 825-828, 200002
  146. Molecular analysis of polyphosphate accumulation in bacteria, BIOCHEMISTRY-MOSCOW, 65(3), 304-308, 200003
  147. Identification and characterization of two chemotactic transducers for inorganic phosphate in Pseudomonas aeruginosa, JOURNAL OF BACTERIOLOGY, 182(12), 3400-3404, 200006
  148. Mutational analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 64(8), 1754-1757, 200008
  149. Involvement of an extracellular protease in algicidal activity of the marine bacterium Pseudoalteromonas sp strain A28, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(10), 4334-4339, 200010
  150. Evaluation of phosphate removal from water by immobilized phosphate-binding protein PstS, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 90(6), 688-690, 200012
  151. Isolation and characterization of the Enterobacter cloacae cheR mutant defective in phosphate taxis, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 65(2), 456-458, 200102
  152. The synthesis of optically pure enantiomers of N-acyl-homoserine lactone autoinducers and their analogues, CHEMISTRY LETTERS, 314-315, 20010405
  153. Chemotaxis of the nematode Caenorhabditis elegans toward cycloheximide and quinine hydrochloride, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 91(3), 322-324, 200103
  154. ★, Role of inorganic polyphosphate in promoting ribosomal, protein degradation by the ion protease in E-coli, SCIENCE, 293(5530), 705-708, 20010727
  155. A sensitive method for detecting AMP by utilizing polyphosphate-dependent ATP regeneration and bioluminescence reactions, BIOCHEMICAL ENGINEERING JOURNAL, 9(3), 193-197, 20011220
  156. Isolation and characterization of cbbL and cbbS genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66(3), 632-635, 200203
  157. A simple method to release polyphosphate from activated sludge for phosphorus reuse and recycling, BIOTECHNOLOGY AND BIOENGINEERING, 78(3), 333-338, 20020505
  158. Behavioral responses of the ciliated protozoan Paramecium caudatum to 2,4-dichlorophenoxyacetic acid and its analogues, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93(4), 416-420, 200204
  159. Cloning and characterization of extracellular metal protease gene of the algicidal marine bacterium Pseudoalteromonas sp strain A28, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 66(6), 1366-1369, 200206
  160. Accumulation of inorganic polyphosphate in phoU mutants of Escherichia coli and Synechocystis sp strain PCC6803, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 68(8), 4107-4110, 200208
  161. Evaluation of bacterial aerotaxis for its potential use in detecting the toxicity of chemicals to microorganisms, JOURNAL OF BIOTECHNOLOGY, 101(1), 11-18, 20030227
  162. The effects of cyclodextrins on autoinducer activities of quorum sensing in Pseudomonas aeruginosa, JOURNAL OF INCLUSION PHENOMENA AND MACROCYCLIC CHEMISTRY, 44(1-4), 381-382, 200212
  163. Molecular mechanism of polyphosphate accumulation in bacteria and its application - Monograph, SEIBUTSU-KOGAKU KAISHI, 81(3), 104-111, 2003
  164. A method for screening polyphosphate-accumulating mutants which remove phosphate efficiently from synthetic wastewater, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 95(6), 637-640, 200306
  165. Strictly polyphosphate-dependent glucokinase in a polyphosphate-accumulating bacterium, Microlunatus phosphovorus, JOURNAL OF BACTERIOLOGY, 185(18), 5654-5656, 200309
  166. Mutants of the nematode Caenorhabditis elegans that are defective specifically in their attraction to cycloheximide, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 96(2), 149-153, 200308
  167. Pilot plant tests on the novel process for phosphorus recovery from municipal wastewater, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 36(10), 1143-1146, 200310
  168. ATP amplification for ultrasensitive bioluminescence assay: Detection of a single bacterial cell, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 68(6), 1216-1220, 200406
  169. Chemotaxis proteins and transducers for aerotaxis in Pseudomonas aeruginosa, FEMS MICROBIOLOGY LETTERS, 231(2), 247-252, 20040216
  170. The aerotaxis transducer gene aer, but not aer-2, is transcriptionally regulated by the anaerobic regulator ANR in Pseudomonas aeruginosa, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(3), 184-190, 200403
  171. New basic and applied aspects of polyphosphate in microorganisms, NIPPON NOGEIKAGAKU KAISHI-JOURNAL OF THE JAPAN SOCIETY FOR BIOSCIENCE BIOTECHNOLOGY AND AGROCHEMISTRY, 78(8), 738-743, 2004
  172. Effects of inorganic polyphosphate on the proteolytic and DNA-binding activities of Lon in Escherichia coli, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(33), 34406-34410, 20040813
  173. A laboratory-scale test of anaerobic digestion and methane production after phosphorus recovery from waste activated sludge, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 97(6), 365-368, 200406
  174. Expression of Pseudomonas aeruginosa aer-2, one of two aerotaxis transducer genes, is controlled by RpoS, JOURNAL OF BACTERIOLOGY, 187(4), 1533-1535, 200502
  175. Isolation and characterization of benzene-tolerant Rhodococcus opacus strains, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 378-382, 200504
  176. Identification of chemosensory proteins for trichloroethylene in Pseudomonas aeruginosa, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 396-402, 200504
  177. Development of a genetic transformation system for benzene-tolerant Rhodococcus opacus strains, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 99(4), 408-414, 200504
  178. Isolation and characterization of solvent-tolerant Pseudomonas putida strain T-57, and its application to biotransformation of toluene to cresol in a two-phase (organic-aqueous) system, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 32(11-12), 542-547, 200512
  179. A polyphosphate-Lon protease complex in the adaptation of Escherichia coli to amino acid starvation, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70(2), 325-331, 200602
  180. Transcriptional analysis of the multicopy hao gene coding for hydroxylamine oxidoreductase in Nitrosomonas sp strain ENI-11, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 70(8), 1875-1881, 200608
  181. Identification and characterization of the chemotactic transducer in Pseudomonas aeruginosa PAO1 for positive chemotaxis to trichloroethylene, JOURNAL OF BACTERIOLOGY, 188(18), 6700-6702, 200609
  182. The Si-Tag for immobilizing proteins on a silica surface, BIOTECHNOLOGY AND BIOENGINEERING, 96(6), 1023-1029, 20070415
  183. Inorganic polyphosphate stimulates Lon-mediated proteolysis of nucleoid proteins in Escherichia coli, CELLULAR AND MOLECULAR BIOLOGY, 52(4), 22-29, 2006
  184. Effect of mineral elements on phosphorus release from heated sewage sludge, BIORESOURCE TECHNOLOGY, 98(13), 2533-2537, 200709
  185. Increase in bioluminescence intensity of firefly luciferase using genetic modification, ANALYTICAL BIOCHEMISTRY, 366(2), 131-136, 20070715
  186. Evaluation of cell wall binding domain of Staphylococcus aureus autolysin as affinity reagent for bacteria and its application to bacterial detection, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 104(1), 55-61, 200707
  187. Performance of mice in discrimination of liquor odors: Behavioral evidence for olfactory attention, CHEMICAL SENSES, 33(3), 283-290, 200803
  188. Use of an Escherichia coli recombinant producing thermostable polyphosphate kinase as an ATP regenerator to produce fructose 1,6-diphosphate, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 73(17), 5676-5678, 200709
  189. Ethylene chemotaxis in Pseudomonas aeruginosa and other Pseudomonas species, MICROBES AND ENVIRONMENTS, 22(2), 186-189, 2007
  190. ★, Detection of chrysotile asbestos by using a chrysotile-binding protein, BIOTECHNOLOGY AND BIOENGINEERING, 99(2), 285-289, 20080201
  191. Enhanced dibenzothiophene biodesulfurization in a microchannel reactor, BIOTECHNOLOGY LETTERS, 30(3), 451-454, 200803
  192. Single bacterial cell detection using a mutant luciferase, BIOTECHNOLOGY LETTERS, 30(6), 1051-1054, 200806
  193. Continuous-flow ATP amplification system for increasing the sensitivity of quantitative bioluminescence assay, ANALYTICAL BIOCHEMISTRY, 379(1), 116-120, 20080801
  194. Pseudomonas aeruginosa as a model microorganism for investigation of chemotactic behaviors in ecosystem, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(1), 1-7, 200807
  195. Fabrication of Si Nanowire Field-Effect Transistor for Highly Sensitive, Label-Free Biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 48(6), 200906
  196. Detection of living Salmonella cells using bioluminescence, BIOTECHNOLOGY LETTERS, 31(5), 737-741, 200905
  197. Si Ring Optical Resonators for Integrated On-Chip Biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 48(4), 200904
  198. High-Sensitivity Detection of ATP Using Bioluminescence at an Optical Fiber End, ELECTRONICS AND COMMUNICATIONS IN JAPAN, 92(9), 53-59, 200909
  199. The Silicon Layer Supports Acid Resistance of Bacillus cereus Spores, JOURNAL OF BACTERIOLOGY, 192(1), 111-116, 201001
  200. Stable polyphosphate accumulation by a pseudo-revertant of an Escherichia coli phoU mutant, BIOTECHNOLOGY LETTERS, 35(5), 695-701, 201305
  201. ★, A New Subfamily of Polyphosphate Kinase 2 (Class III PPK2) Catalyzes both Nucleoside Monophosphate Phosphorylation and Nucleoside Diphosphate Phosphorylation, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 80(8), 2602-2608, 201404
  202. Affinity purification of recombinant proteins using a novel silica-binding peptide as a fusion tag, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(12), 5677-5684, 201406
  203. Application of a phosphite dehydrogenase gene as a novel dominant selection marker for yeasts, JOURNAL OF BIOTECHNOLOGY, 182, 68-73, 20140720
  204. Automated enzyme-linked immunosorbent assay using beads in a single tip (BIST) technology coupled with a novel anchor protein for oriented antibody immobilization, ANALYTICAL METHODS, 6(16), 6232-6235, 20140821
  205. Development of an automated asbestos counting software based on fluorescence microscopy, ENVIRONMENTAL MONITORING AND ASSESSMENT, 187(1), 201501
  206. A BRET-Based Homogeneous Insulin Assay Using Interacting Domains in the Primary Binding Site of the Insulin Receptor, ANALYTICAL CHEMISTRY, 87(5), 2764-2770, 20150303
  207. A FRET-based DNA nano-tweezer technique for the imaging analysis of specific mRNA, ANALYST, 140(4), 999-1003, 2015
  208. A split G-quadruplex-based DNA nano-tweezers structure as a signal-transducing molecule for the homogeneous detection of specific nucleic acids, BIOSENSORS & BIOELECTRONICS, 74, 222-226, 20151215
  209. Porphyrin-uptake in liposomes and living cells using an exchange method with cyclodextrin, RSC ADVANCES, 5(127), 105279-105287, 2015
  210. The C-Terminal Zwitterionic Sequence of CotB1 Is Essential for Biosilicification of the Bacillus cereus Spore Coat, JOURNAL OF BACTERIOLOGY, 198(2), 276-282, 201601
  211. Detection of antibody-antigen reaction by silicon nitride slot-ring biosensors using protein G, OPTICS COMMUNICATIONS, 365, 16-23, 20160415
  212. Surface Modification of Cell Scaffold in Aqueous Solution Using TiO2 Photocatalysis and Linker Protein L2 for Patterning Primary Neurons, E-JOURNAL OF SURFACE SCIENCE AND NANOTECHNOLOGY, 13, 213-218, 2015
  213. Differential Si ring resonators for label-free biosensing, JAPANESE JOURNAL OF APPLIED PHYSICS, 55(4), 201604
  214. A Lactobacillus mutant capable of accumulating long-chain polyphosphates that enhance intestinal barrier function, BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY, 80(5), 955-961, 201605
  215. Rapid on-site detection of airborne asbestos fibers and potentially hazardous nanomaterials using fluorescence microscopy-based biosensing, BIOTECHNOLOGY JOURNAL, 11(6), 757-767, 201606
  216. Continuous Monitoring of Specific mRNA Expression Responses with a Fluorescence Resonance Energy Transfer-Based DNA Nano-tweezer Technique That Does Not Require Gene Recombination, ANALYTICAL CHEMISTRY, 88(16), 7894-7898, 20160816
  217. Application of volcanic ash particles for protein affinity purification with a minimized silica-binding tag, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 122(5), 633-638, 201611
  218. Differential Counting of Asbestos Using Phase Contrast and Fluorescence Microscopy, ANNALS OF OCCUPATIONAL HYGIENE, 60(9), 1104-1115, 201611
  219. Engineering of Escherichia coli to facilitate efficient utilization of isomaltose and panose in industrial glucose feedstock, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 101(5), 2057-2066, 201703
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Publications such as books

  1. 2000/00, Molecular genetics of bacterial polyphosphate accumulation to better understand the mechanism underlying biological phosphorus removal. , 2000, 00, Scholarly Book, Joint work, 15
  2. 2003/00, Applications of polyphosphate and other biopolmers in wastewater treatment. , 2003, 00, Scholarly Book, Joint work, 18
  3. 1999/02, New current of biotechnology , 1999, 02, Textbook, Cocompilation, 4254171064, 156
  4. 1996, Chemotactic signal transduction network in Pseudomonas aeruginosa , Molecular Biology of Pseudomonads. American Society for Microbiology= Washington USA, 1996
  5. 1999, Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater , Inorganic polyphosphates, 1999
  6. 1999, Progress in Molecular and Subcellar Biology, --Genetic improvement of bacteria for enhanced biological removal of phosphate from wastewater , Springer-Verlag, 1999, Joint work
  7. 2003, Applications of polyphosphate and other biopolmers in wastewater treatment , Wiley-VCHA= "Biopolymers: Vol.10 General Aspects and Special "Biopolymers: Vol.10 General Aspects and Special Biopolymers: Vol.10 General Aspects and Special Applications (A. Steinbuchel [ed.]), 2003, Scholarly Book, Joint work, 18
  8. 1997, An Approach to Molecular Artificial Life: bacterial intelligent behavior and its computer modelin Artificial Life V (C. G. Langton and K. Shimohara, Ed.), Artificial Life V, 1997, Scholarly Book, Joint work, 7
  9. 2004/11, Basic molecular biology for biotechnology , 2004, 11, Textbook, Cocompilation, 4759809686, 199
  10. 2008/08, ATP regeneration and biological production of phosphorylated compounds using thermostable polyphosphate kinase, 2008, 08, Cocompilation, 8
  11. 2012/02, FIBER OPTIC SENSOR Chapter 19: High-sensitivity Detection of Bioluminescnece at an Optical Fiber End for an ATP sensor, 2012, 02, Scholarly Book, Cocompilation, 9789533079226, 518

Awards

  1. 1995/10, "Human frontier science program, Long-term fellowship award."