KENJI TOYOTA

Last Updated :2024/12/03

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Assistant Professor
E-mail
toyotakhiroshima-u.ac.jp
Self-introduction
My group is conducting eco-physiological research with a focus on crustaceans. We are exploring endocrine research aimed at achieving high growth of useful fishery species such as the horsehair crab and snow crab, and approaches to apply these findings to understanding the unique life phenomena exhibited by coastal crustaceans. We also aim to construct basic data that will contribute to the conservation of ecosystems and biodiversity assessment, not only in coastal areas, but also in the interconnectedness of forests, villages, oceans, and rivers.

Basic Information

Major Professional Backgrounds

  • 2024/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Assistant Professor
  • 2022/10/01, 2024/03/31, Noto Marine Laboratory, Institute of Nature and Environmental Technology, Kanazawa University, Specially Appointed Assistant Professor
  • 2020/06/22, 2022/09/30, Marine Biological Station, Sado Island Center for Ecological Sustainability, Niigata University, Specially Appointed Assistant Professor
  • 2018/05/01, Tokyo University of Science, A visiting researcher
  • 2018/04/01, Research Institute for Integrated Science, Kanagawa University, A visiting researcher
  • 2018/04/01, 2018/09/30, Kanagawa University, A part-time lecturer
  • 2018/04/01, 2020/06/21, Kanagawa University, JSPS postdoctoral fellow PD
  • 2017/08/25, 2018/03/31, Wakayama Medical University, Postdoctoral Fellow
  • 2016/02/01, 2017/08/24, University of Birmingham, JSPS postdoctoral fellowship for research abroad
  • 2015/04/01, 2016/01/31, National Institute for Basic Biology, NIBB research fellow
  • 2012/04/01, 2015/03/31, National Institute for Basic Biology, The Graduate University for Advanced Studies (SOKENDAI), JSPS research fellow DC1
  • 2019/04/01, 2019/09/30, Kanagawa University, A part-time lecturer

Educational Backgrounds

  • The Graduate University for Advanced Studies (SOKENDAI), School of Life Science, Department of Basic Biology, Japan, 2010/04/01, 2015/03/31
  • Niihama National College of Technology, Advanced Engineering Course of Applied Chemistry and Biotechnology, Japan, 2008/04/01, 2010/03/31
  • Niihama National College of Technology, Department of Applied chemistry and Biotechnology, Japan, 2003/04/01, 2008/03/31

Academic Degrees

  • The Graduate University for Advanced Studies

Research Fields

  • Biology;Basic biology;Morphology / Structure
  • Biology;Basic biology;Animal physiology / Animal behavior
  • Biology;Basic biology;Evolutionary biology
  • Biology;Basic biology;Biodiversity / Systematics
  • Biology;Basic biology;Ecology / Environment
  • Agricultural sciences;Applied aquatic science;Aquatic life science
  • Agricultural sciences;Applied aquatic science;Aquatic bioproduction science

Research Keywords

  • Crustacean; Invertebrate; Linkage of forests, villages, oceans, and rivers, Islands; Seto Inland Sea; Sea of Japan; Sex differences; Biodiversity

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, Intensive, Laboratory Work in Hydrospheric BiologyII
  2. 2024, Undergraduate Education, 1Term, Exercises in Integrative Hydrospheric ScienceI
  3. 2024, Undergraduate Education, Second Semester, Graduation Thesis I
  4. 2024, Undergraduate Education, First Semester, Graduation Thesis II
  5. 2024, Undergraduate Education, Second Semester, Graduation Thesis III
  6. 2024, Undergraduate Education, Intensive, (AIMS) Fish Production

Research Activities

Academic Papers

  1. Identification and physiological assays of crustacean hyperglycemic hormones in the Japanese spiny lobster Panulirus japonicus., Zoological Science, 41, 14-20, 202402
  2. Gender identification of the horsehair crab, Erimacrus isenbeckii (Brandt, 1848), by image recognition with a deep neural network., Scientific Reports, 13, 19190, 20231113
  3. Fluorene is highly toxic to zoea larvae of the red-clawed crab Chiromantes haematocheir., International Journal of Zoological Investigations, 9, 1-7, 2023
  4. Analysis of seawater toxicity at five concentrated seawater discharge sites in Korea using a goldfish scale-based in vitro bioassay system., International Journal of Biological and Environmental Investigations., 3, 1-8, 2023
  5. Stanniocalcin in the corpuscles of stannius inhibits the osteoclastic activation by regulating the rankl/opg expression., Journal of Biological Regulators and Homeostatic Agents., 37, 5141-5149, 20231020
  6. Comparative analysis of gonadal transcriptomes between turtle and alligator identifies common molecular cues activated during the temperature-sensitive period for sex determination., Gene, 20231220
  7. ★, Dual roles of crustacean female sex hormone during juvenile stage in the kuruma prawn Marsupenaeus japonicus., General and Comparative Endocrinology, 344, 114374, 20231201
  8. Sacculina-induced morphological feminization in the grapsid crab, Pachygrapsus crassipes., Zoological Science, 40, 367-374, 202310
  9. Sexual dimorphic eyestalk transcriptome of kuruma prawn Marsupenaeus japonicus., Gene, 885, 14770, 20231115
  10. The role of mesenchymal estrogen receptor 1 in mouse uterus in response to estrogen., Scientific Reports, 13, 12293, 20230729
  11. Data on plasma cortisol levels in nibbler fish Girella punctata reared under high-density conditions in either surface seawater or deep ocean water., Data in Brief, 49, 103961, 20230704
  12. Dietary effects on larval survival and development of three sesarmid crabs., Plankton and Benthos Research, 18, 84-92, 20230531
  13. Kynurenine promotes Calcitonin secretion and reduces cortisol in the Japanese flounder Paralichthys olivaceus., Scientific Reports, 13, 8700, 20230529
  14. Deep ocean water alters the cholesterol and mineral metabolism of squid Todarodes pacificus and suppresses its weight loss., Scientific Reports, 13, 7591, 20230510
  15. ★, Eyestalk transcriptome and methyl farnesoate titers provide insight into the physiological changes in the male snow crab, Chionoecetes opilio, after its terminal molt., Scientific Reports, 13, 7204, 20230503
  16. Adaptation to the shallow sea-floor environment of a species of marine worms, Oligobrachia mashikoi, generally inhabiting deep-sea water., Scientific Reports, 13, 6299, 20230418
  17. Functional analysis of a matrix peptide involved in calcification of the exoskeleton of the kuruma prawn, Marsupenaeus japonicus., Aquaculture, 559, 738437, 20221015
  18. Purification and characterization of red pigment concentrating hormone from the Alaska pink shrimp Pandalus eous., Aquatic Animals, AA2022-8., 2022
  19. Laterally biased diffusion of males of the water flea Daphnia magna., Journal of Experimental Zoology Part A, 337, 626-638, 202207
  20. Uncovering the role of endocrine factors in decapod physiology., Impact, 4, 23-24, 2022
  21. Juvenile hormone synthesis and signaling disruption triggering male offspring induction and population decline in cladocerans (water flea) : Review and adverse outcome pathway development., Aquatic Toxicology, 243, 106058, 2022
  22. Chemical synthesis and functional evaluation of the crayfish insulin-like androgenic gland factor, Bioorganic Chemistry, 122, 105738, 202205
  23. Effect of blue light on the growth of the red swamp cray fish Procambraus clarkii larvae -seasonal and sexual differences-, Zoological Studies, 61, 3, 20220223
  24. Annual reproductive cycle of the greasyback shrimp Metapenaeus ensis in Ise Bay, Japan., Fisheries Science, 88, 63-73, 20211129
  25. Novel validating indices to indicate sexual differences in the horsehair crab Erimacrus isenbeckii (Brandt, 1848)., Aquatic Animals, AA2021-6, 2021
  26. Sex determination and differentiation in decapod and cladoceran crustaceans: an overview of endocrine regulation., Genes, 12, 305, 2021
  27. Photoperiod-dependent reproductive plasticity of the brine shrimp Artemia franciscana., Aquatic Animals, AA2020-3, 2021
  28. Characterization of G protein-coupled estrogen receptors in Japanese medaka, Oryzias latipes, Journal of Applied Toxicology, 41, 1390-1399, 202109
  29. Methyl farnesoate regulatory mechanisms underlying photoperiod-dependent sex determination in the freshwater crustacean Daphnia magna., Journal of Applied Toxicology, 41, 216-223, 202102
  30. Molecular insights into structural and ligand binding features of Methoprene-tolerant in daphnids., Chemical Research in Toxicology, 33, 2785-2792, 20201116
  31. Morphometric approaches reveal sexual differences in the carapace shape of the horsehair crab, Erimacrus isenbeckii (Brandt, 1848)., Aquatic Animals, AA2020-1, 2020
  32. Estrogen receptor 1 (ESR1) agonist induces ovarian differentiation and aberrant Müllerian duct development in Chinese soft-shelled turtle, Pelodiscus sinensis, Zoological Studies, 59, 54, 20201111
  33. ★, Impacts of methyl farnesoate and 20-hydroxyecdysone on larval mortality and metamorphosis in the kuruma prawn Marsupenaeus japonicus, Frontiers in Endocrinology, 11, 475, 20200728
  34. Transgenerational response to early spring warming in Daphnia., Scientific Reports, 9, 4449, 20190314
  35. Functional distinctions associated with the diversity of sex steroid hormone receptors ESR and AR., The Journal of Steroid Biochemistry and Molecular Biology, 184, 38-46, 2018
  36. Photoperiodism of male offspring production in the water flea Daphnia pulex., Zoological Science, 34, 312-317, 201708
  37. Ecdysone receptor agonism leading to lethal molting disruption in arthropods: Review and adverse outcome pathway development., Environmental Science & Technology, 51, 4142-4157, 2017
  38. Comparative ovarian microarray analysis of juvenile hormone-responsive genes in water flea Daphnia magna: potential targets for toxicity., Journal of Applied Toxicology, 37, 374-381, 201703
  39. Protein kinase C is involved with upstream signaling of methyl farnesoate for photoperiod-dependent sex determination in the water flea Daphnia pulex., Biology Open, 6, 161-164, 20170215
  40. Toxic effects of chemical dispersant Corexit 9500 on water flea Daphnia magna, Journal of Applied Toxicology, 37, 201-206, 201702
  41. Neverland regulates embryonic moltings through the regulation of ecdysteroid synthesis in the water flea Daphnia magna, and may thus act as a target for chemical disruption of molting, Journal of Applied Toxicology, 36, 1476-1485, 201611
  42. ★, Metabolomics reveals an involvement of pantothenate for male production responding to the short-day stimulus in the water flea, Daphnia pulex., Scientific Reports, 6, 25125, 20160426
  43. Comparative developmental staging of the female and male water fleas Daphnia pulex and Daphnia magna during embryogenesis., Zoological Science, 33, 31-37, 201602
  44. RFLP analysis reveals the genealogical relation of novel Japanese persimmon Saijo., 199, 71-74, 20160216
  45. ★, Methyl farnesoate synthesis is necessary for the environmental sex determination in water flea Daphnia pulex., Journal of Insect Physiology, 80, 22-30, 201509
  46. ★, NMDA receptor activation upstream of methyl farnesoate signaling for short day-induced male offspring production in the water flea, Daphnia pulex., BMC Genomics, 16, 186, 20150514
  47. Diofenolan induces male offspring production through binding to juvenile hormone receptor in Daphnia magna., Aquatic Toxicology, 159, 44-51, 201502
  48. Targeted gene disruption by use of transcription activator-like effector nuclease (TALEN) in the water flea Daphnia pulex., BMC Biotechnology, 14, 95, 20141118
  49. Roles of ecdysteroids for progression of reproductive cycle in the fresh water crustacean Daphnia magna., Frontiers in Zoology, 11, 60, 20140819
  50. Molecular impact of juvenile hormone agonists on neonatal Daphnia magna., Journal of Applied Toxicology, 34, 537-544, 20130905
  51. Comparison of JH signaling in insects and crustaceans., Current Opinion in Insect Science, 1, 81-87, 2014
  52. Development of microinjection system for RNA interference in the water flea Daphnia pulex., BMC Biotechnology, 13, 96, 20131105
  53. A mutation in the receptor Methoprene-tolerant alters juvenile hormone response in insects and crustaceans, Nature Communications, 4, 1856, 20130514
  54. Molecular cloning of doublesex genes of four cladocera (water flea) species., BMC Genomics, 14, 239, 20130410

Publications such as books

  1. 2023/11/08, Arthropods - New Advances and Perspective, Larval development of non-insect Arthropods: Metamorphosis and sexual differentiation, Scholarly Book, Joint work, English, Kenji Toyota, Yuta Sakae, Taisen Iguchi.
  2. 2018, Reproductive and Developmental Strategies: the Continuity of Life. Part 2: Diversity in Sex Differentiation., Environmental control on sex differentiation in Daphnia, Scholarly Book, Joint work, English, Kenji Toyota, Norihisa Tatarazako, Taisen Iguchi.
  3. 2016/04/06, RNA interference, Microinjection-based RNA interference method in the water flea, Daphnia pulex and Daphnia magna., Scholarly Book, Joint work, English, Kenji Toyota, Shinichi Miyagawa, Yukiko Ogino, Taisen Iguchi.
  4. Daphnia: Biology and Mathmatics Perspectives, Sexual reproduction is a key element in the life history strategy of water fleas, Daphnia magna and Daphnia pulex. -Casting a spotlight on male induction and its morphology-, 2014, 1, Scholarly Book, Joint work, English, Chizue Hiruta, Kenji Toyota, Hitoshi Miyakawa, Eri Sumiya, Taisen Iguchi.

External Funds

Acceptance Results of Competitive Funds

  1. 2024/07/01, 2025/03/31
  2. 2024/07/01, 2026/03/31
  3. 2024/04/01, 2027/03/31
  4. 2024/04/01, 2027/03/31
  5. 2024/04/01, 2028/03/31
  6. 2023/04/01, 2027/03/31
  7. 2023/06/01, 2024/04/01
  8. 2022/04/01, 2026/03/31
  9. 2020/09/01, 2022/03/31
  10. 2020/04/01, 2023/03/31
  11. 2021/04/01, 2024/03/31
  12. 2019/04/01, 2020/03/31
  13. 2019/04/01, 2020/03/31
  14. 2018/04/01, 2021/03/31
  15. 2018/04/01, 2020/03/31
  16. 2015/04/01, 2016/03/31
  17. 2012/04/01, 2015/03/31
  18. 2011/04/01, 2012/03/31
  19. 2011/04/01, 2012/03/31