Norio Sakai

Last Updated :2024/07/05

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Medicine), Professor
E-mail
nsakaihiroshima-u.ac.jp
Self-introduction
My research concern is neuropharmacology. Especially, I am interested in the functions of protein kinase C and serotonin transporter in central nervous system.

Basic Information

Major Professional Backgrounds

  • 1993/04/01, 1994/05/31, Japan Society for the Promotion of Science, Researcher
  • 1994/06/01, 1999/10/31, Kobe University, Research Associate
  • 1999/11/01, 2002/03/31, Kobe University, Associate Professor
  • 2002/04/01, 2019/03/31, Hiroshima University, Graduate School of Biomedical Sciences, Professor
  • 2019/04/01, Hiroshima University, Graduate School of Biomedical & Health Sciences, Professor

Educational Backgrounds

  • Kobe University, Graduate School, Division of Medicine, Dept of Pharmacology, Japan, 1989/04, 1993/03
  • Kobe University, Faculty of Medicine, Japan, 1979/04, 1986/03

Academic Degrees

  • Doctor of Philosophy in Medical Science, Kobe University

Educational Activity

  • [Bachelor Degree Program] School of Medicine : Program of Medicine : Medicine
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Biomedical Science
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Biomedical Sciences : Program of Medicine

In Charge of Primary Major Programs

  • Medicine

Research Fields

  • Medicine,dentistry, and pharmacy;Basic medicine;General pharmacology

Research Keywords

  • neural plasticity
  • neuropsychiatric disease
  • cell death
  • protein kinase
  • phosphorylation
  • spinno cerebellar ataxia
  • serotonin transporter
  • neurodegenerative disease
  • Protein kinase C

Affiliated Academic Societies

  • society for neuroscience, 1995
  • The Japanese Society of Clinical Pharmacology and Therapeutics
  • Japan Society for Medical education, 2017/04

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, 4Term, Exercise in Medical and Clinical Engineering 1
  2. 2024, Undergraduate Education, Intensive, Exercise in Medical and Clinical Engineering 2
  3. 2024, Undergraduate Education, Year, Biological Responses
  4. 2024, Undergraduate Education, 2Term, Introduction for medical research
  5. 2024, Undergraduate Education, Year, Methodology in advanced medical sciences
  6. 2024, Undergraduate Education, 4Term, Medical Neuroscience I
  7. 2024, Undergraduate Education, Intensive, Medical Neuroscience II
  8. 2024, Undergraduate Education, Year, Medical Neuroscience III
  9. 2024, Undergraduate Education, Intensive, Practice for medical research
  10. 2024, Undergraduate Education, 2Term, Clinical pharmacology
  11. 2024, Undergraduate Education, 2Term, Clinical pharmacology
  12. 2024, Undergraduate Education, 2Term, Clinical pharmacology
  13. 2024, Graduate Education (Master's Program) , First Semester, Seminar
  14. 2024, Graduate Education (Master's Program) , Second Semester, Seminar
  15. 2024, Graduate Education (Master's Program) , First Semester, Research
  16. 2024, Graduate Education (Master's Program) , Second Semester, Research
  17. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
  18. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
  19. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research
  20. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
  21. 2024, Graduate Education (Master's Program) , 4Term, General Pharmacology
  22. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Molecular and Pharmacological Neuroscience
  23. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Molecular and Pharmacological Neuroscience
  24. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Molecular and Pharmacological Neuroscience
  25. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Molecular and Pharmacological Neuroscience

Research Activities

Academic Papers

  1. S-Palmitoylation of the serotonin transporter promotes its cell surface expression and serotonin uptake, Biochemical and Biophysical Research Communications, 662, 58-65, 2023
  2. Features and mechanisms of propofol-induced protein kinase C (PKC) translocation and activation in living cells, FRONTIERS IN PHARMACOLOGY, 14, 20231107
    Link to Paper Search Results by DOI
  3. Identification of protein kinase C domains involved in its translocation induced by propofol, EUROPEAN JOURNAL OF PHARMACOLOGY, 955, 20230915
    Link to Paper Search Results by DOI
  4. P2Y2 receptor mediates dying cell removal via inflammatory activated microglia, JOURNAL OF PHARMACOLOGICAL SCIENCES, 153(1), 55-67, 202309
    Link to Paper Search Results by DOI
  5. N-methyl-D-aspartate receptors and glycinergic transmission, respectively, mediate muscle relaxation and immobility of pentobarbital in mice, NEUROSCIENCE LETTERS, 802, 20230401
    Link to Paper Search Results by DOI
  6. Uptake of Staphylococcus aureus by keratinocytes is reduced by interferon-fibronectin pathway and filaggrin expression, JOURNAL OF DERMATOLOGY, 49(11), 1148-1157, 202211
    Link to Paper Search Results by DOI
  7. Comparison of two families with and without ataxia harboring novel variants in PRKCG, JOURNAL OF HUMAN GENETICS, 67(10), 595-599, 202210
    Link to Paper Search Results by DOI
  8. GPR3 expression in retinal ganglion cells contributes to neuron survival and accelerates axonal regeneration after optic nerve crush in mice, NEUROBIOLOGY OF DISEASE, 172, 20221001
    Link to Paper Search Results by DOI
  9. Characterization of intracellular calcium mobilization induced by remimazolam, a newly approved intravenous anesthetic., PLos One, 17(2), e0263395, 20220201
    Link to Paper Search Results by DOI
  10. Potential role of inducible GPR3 expression under stimulated T cell conditions., J. Pharmacol. Sci, 148(3), 307-314, 20220113
    Link to Paper Search Results by DOI
  11. Mutant PKC gamma in Spinocerebellar Ataxia Type 14 Disrupts Synapse Elimination and Long-Term Depression in Purkinje Cells In Vivo, JOURNAL OF NEUROSCIENCE, 31(40), 14324-14334, 20111005
    Link to Paper Search Results by DOI
  12. Effects of L-threo-DOPS, a noradrenaline precursor, on the long-term potentiation in the rat hippocampal mossy fiber-CA3 region., Brain Research, 567, 267-273, 19910401
  13. Inhibitory modulation of long-term potentiation via the 5-HT_1A_ receptor in slices of the rat hippocampal dentate gyrus., Brain Research, 613, 326-330, 19930401
  14. A role for potassium and calcium channels in induction of long-lasting synaptic enhancement by calyculin A in rat hippocampal CA1 region., Neurosci. Lett., 176, 181-184, 19940401
  15. Presynaptic and Ca^2+^-independent PKC subspecies modulates NMDAR1 current., NeuroReport, 7, 477-480, 19960401
  16. Neuronal and glial localization of two GABA transporters ( GAT1 and GAT3) in the rat cerebellum., Mol. Brain Res., 37, 309-316, 19960401
  17. Electrophysiological and pharmacological characteristics of ionotropic glutamate receptors in medial vestibular nucleus neurons: A whole cell patch clamp study in acutely dissociated neurons., Jpn J Pharmacol, 72, 335-346, 19960401
  18. Modulation of serotonin transporter activity by a PKC activator and an inhibitor of type1 and 2A serine/threonine phosphatases., J. Neurochem., 68, 2618-2624, 19970401
  19. GABAergic neurotransmission in rat taste buds; Immunocytochemical study for GABA and GABA traspoter subtypes., Mol. Brain Res., 49, 29-36, 19970401
  20. Effects of tricyclic and tetracyclic antidepressants on the three subtypes of GABAtransporter., Neurosci. Res., 29, 87-91, 19970401
  21. Three distinct mechanisms for translocation and activation of δ-subspecies of protein kinase C., Mol. Cell. Biol., 18, 5263-5271, 19980401
  22. Transgenic animals with inducible targeted gene expression in brain., Mol Pharmacol, 54, 495-503, 19980401
  23. Subspecies-specific targeting mechanism of protein kinase C., Jpn J Pharmacol, 78, 411-417, 19980401
  24. Association study of a polymorphsim of nonerythroid a-spectrin gene with schizophrenia., Am. J. Med. Genet. (Neuropsychiatr. Genet.), 88, 291-293, 19990401
  25. Advanced glycation end product (AGE) inhibition as a possible pharmacotherapeutic strategy in the treatment of Alzheimer’s disease., Drugs Fut., 24, 411-416, 19990401
  26. Polymorphism of the apolipoproteinE promoter and bleomycin hydrolase gene: Risk factors for Alzheimer’s dementia ?, Neurosci. Lett., 274, 37-40, 19990401
  27. Identification and functional analysis of novel CREB splice variants lacking the bZIP domain., Mol Pharmacol, 56, 917-925, 19990401
  28. Induction of 55 kDa protein of PKN by ischemia/reperfusion model of rat retina., Invest. Ophthal. Vis. Sci., 41, 29-35, 20000401
  29. Involvement of the actin cytoskeleton in the regulation of serotonin transporter (SET) activity: possible mechanism underlying SET regulation by protein kinase C., Neurochem. Int., 36, 567-579, 20000401
  30. Phospholipase A2 and its products are involved in the purinergic receptor-mediated translocation of protein kinase C in CHO-K1 cells., J. Cell Sci., 113, 1335-1343, 20000401
  31. Regulation of GFRα-1 and GFRα-2 mRNAs in rat brain by electroconvulsive seizure., Synapse, 39, 42-50, 20010401
  32. cAMP response element-mediated gene transcription is upregulated by chronic antidepressnt treatment., J. Neurosci., 20, 4030-4036, 20000401
  33. Subtype-specific translocation of diacylglycerol kinase α and γ and its correlation with protein kinase C., J. Biol. Chem., 275, 24760-24766, 20000401
  34. Importance of PKC targeting for the phosphorylation of its substrate, myristoylated alanine-rich C-kinase substrate (MARCKS), J. Biol. Chem., 275, 26449-26457, 20000401
  35. Subtype-specific translocation of δ subtype of protein kinase C and its activation by tyrosine phosphorylation induced by ceramide in Hela cells., Mol. Cell. Biol., 21, 1769-1783, 20010401
  36. Generation of a constitutively active fragment of PKN in Microglia/Macrophages after Middle Cerebral Artery Occlusion in Rats., J. Neurochem., 79, 903-913, 20010401
  37. Importance of C1B domain for lipid messenger-induced targeting of PKC., J. Biol. Chem., 277, 18037-18045, 20020401
  38. ★, Inducible and brain region specific CREB transgenic mice, Mol Pharmacol, 61, 1453-1464, 20020401
  39. A new neuromodulator pathway with a glial contribution mediated via A2a adrenergic receptors., Glia, 39, 133-147, 20020401
  40. The silencer activity of the novel human serotonin transporter linked polymorphic region., Neurosci. Lett., 327, 13-16, 20020401
  41. Induction of apoptosis by Protein kinase C delta is independent of its kinase activity., J. Biol. Chem., 277, 18037-17045, 20020401
  42. Subtype- and species-specific knockdown of PKC using short interfering RNA., Biochem. Biophys. Res. Commun., 298, 738-743, 20020401
  43. Inhibition of cAMP response element-binding protein or dynorphin in the nucleus accumbens produces an antidepressant-like effect., J. Neurosci., 24, 10883-10890, 20020401
  44. Novel variants of murine serotonin transporter mRNA and the promoter activity of its upstream site., Neurosci. Lett., 342, 175-178, 20030401
  45. Effects of D-Fraction, a polysaccharide from Grifola frondosa on tumor growth involve activation of NK cells, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 25(12), 1647-1650, 200212
    Link to Paper Search Results by DOI
  46. Ceramide-induced apoptosis by translocation, phosphorylation, and activation of protein kinase C delta in the Golgi complex, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(13), 12668-12676, 20040326
    Link to Paper Search Results by DOI
  47. Superoxide production at phagosomal cup/phagosome through beta I protein kinase C during Fc gamma R-mediated phagocytosis in microglia, JOURNAL OF IMMUNOLOGY, 173(7), 4582-4589, 20041001
    Link to Paper Search Results by DOI
  48. Importance of chroman ring and tyrosine phosphorylation in the subtype-specific translocation and activation of diacylglycerol kinase alpha by D-alpha-tocopherol, GENES TO CELLS, 10(4), 311-319, 200504
    Link to Paper Search Results by DOI
  49. Maitake D-fraction enhances antitumor effects and reduces immunosuppression by mitomycin-C in tumor-bearing mice, NUTRITION, 21(5), 624-629, 200505
    Link to Paper Search Results by DOI
  50. Protein kinase C-alpha a mediates TNF release process in RBL-2H3 mast cells, BRITISH JOURNAL OF PHARMACOLOGY, 145(4), 415-423, 200506
    Link to Paper Search Results by DOI
  51. Effects of continuous administration of paroxetine on ligand binding site and expression of serotonin transporter protein in mouse brain, BRAIN RESEARCH, 1053(1-2), 154-161, 20050816
    Link to Paper Search Results by DOI
  52. Fused protein of delta PKC activation loop and PDK1-interacting fragment (delta AL-PIF) functions as a pseudosubstrate and an inhibitory molecule for PDK1 when expressed in cells, GENES TO CELLS, 11(9), 1051-1070, 200609
    Link to Paper Search Results by DOI
  53. Spatiotemporal analysis of the molecular interaction between PICK1 and PKC, ACTA HISTOCHEMICA ET CYTOCHEMICA, 39(6), 173-181, 2006
    Link to Paper Search Results by DOI
  54. BDNF, NT-3, and NGF released from transplanted neural progenitor cells promote corticospinal axon growth in organotypic cocultures, SPINE, 32(12), 1272-1278, 20070520
    Link to Paper Search Results by DOI
  55. Enzymological analysis of mutant protein kinase C gamma causing spinocerebellar ataxia type 14 and dysfunction in Ca2+ homeostasis, JOURNAL OF BIOLOGICAL CHEMISTRY, 283(28), 19854-19863, 20080711
    Link to Paper Search Results by DOI
  56. Effects of ATP on the release of nitric oxide in microglia, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 127, 193-195, 2007
    Link to Paper Search Results by DOI
  57. N-acetyl-L-aspartate activates hippocampal CA3 neurons in rodent slice preparations, BRAIN RESEARCH BULLETIN, 75(5), 663-667, 20080328
    Link to Paper Search Results by DOI
  58. Signaling and neuroprotective function of alpha 7 nicotinic acetylcholine receptors in microglia, YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 128, 38-40, 2008
    Link to Paper Search Results by DOI
  59. Metabotropic glutamate receptor subtype-1 is essential for in vivo growth of melanoma, ONCOGENE, 27(57), 7162-7170, 200812
    Link to Paper Search Results by DOI
  60. Cell-dependent physiological synaptic action of morphine in the rat habenular nucleus: Morphine both inhibits and facilitates excitatory synaptic transmission, NEUROSCIENCE LETTERS, 451(3), 270-273, 20090227
    Link to Paper Search Results by DOI
  61. mu-Opioid receptor-independent fashion of the suppression of sodium currents by mu-opioid analgesics in thalamic neurons, NEUROSCIENCE LETTERS, 453(1), 62-67, 20090327
    Link to Paper Search Results by DOI
  62. Stimulatory effects of the soy phytoestrogen genistein on noradrenaline transporter and serotonin transporter activity, MOLECULAR NUTRITION & FOOD RESEARCH, 54(4), 516-524, 201004
    Link to Paper Search Results by DOI
  63. Direct binding of RalA to PKC eta and its crucial role in morphological change during keratinocyte differentiation, MOLECULAR BIOLOGY OF THE CELL, 22(8), 1340-1352, 20110415
    Link to Paper Search Results by DOI
  64. JosD1, a Membrane-targeted Deubiquitinating Enzyme, Is Activated by Ubiquitination and Regulates Membrane Dynamics, Cell Motility, and Endocytosis, JOURNAL OF BIOLOGICAL CHEMISTRY, 288(24), 17145-17155, 20130614
    Link to Paper Search Results by DOI
  65. A Brain-specific Grb2-associated Regulator of Extracellular Signal-regulated Kinase (Erk)/Mitogen-activated Protein Kinase (MAPK) (GAREM) Subtype, GAREM2, Contributes to Neurite Outgrowth of Neuroblastoma Cells by Regulating Erk Signaling, JOURNAL OF BIOLOGICAL CHEMISTRY, 288(41), 29934-29942, 20131011
    Link to Paper Search Results by DOI
  66. The Role of Pak-Interacting Exchange Factor-beta Phosphorylation at Serines 340 and 583 by PKC gamma in Dopamine Release, JOURNAL OF NEUROSCIENCE, 34(28), 9268-9280, 20140709
    Link to Paper Search Results by DOI
  67. Identification and characterization of PKCP gamma, a kinase associated with SCA14, as an amyloidogenic protein, HUMAN MOLECULAR GENETICS, 24(2), 525-539, 20150115
    Link to Paper Search Results by DOI
  68. The Piccolo Intronic Single Nucleotide Polymorphism rs13438494 Regulates Dopamine and Serotonin Uptake and Shows Associations with Dependence-Like Behavior in Genomic Association Study, CURRENT MOLECULAR MEDICINE, 15(3), 265-274, 2015
  69. Adenoviral gene transfer of aspartoacylase into the tremor rat, a genetic model of epilepsy, as a trial of gene therapy for inherited epileptic disorder., Neurosci Lett, 328(3), 249-252, 2002
  70. Involvement of Ca(2+) channels in abnormal excitability of hippocampal CA3 pyramidal cells in noda epileptic rats., J. Pharmacol. Sci., 91(2), 2003
  71. Repeated administration of methamphetamine causes hypersensitivity of D2 receptor in rat ventral tegmental area., Neurosci. Lett., 347(2), 2003
  72. Perospirone, a novel antipsychotic agent, hyperpolarizes rat dorsal raphe neurons via 5-HT1A receptor., J. Pharmacol. Sci., 93(1), 114-117, 2003
  73. Electrophysiological characterization of nicotine-induced excitation of dopaminergic neurons in the rat substantia nigra., J. Pharmacol. Sci., 93(2), 143-148, 2003
  74. Postsynaptic alpha 4 beta 2 and alpha 7 type nicotinic acetylcholine receptors contribute to the local and endogenous acetylcholine-mediated synaptic transmissions in nigral dopaminergic neurons., J. Pharmacol. Sci., 1005(1-2), 143-148, 2004
  75. Adenoviral gene transfer of aspartoacylase ameliorates tonic convulsions of spontaneously epileptic rats., Neurochem. Int., 45(1), 171-178, 2004
  76. Antiepileptic effects of single and repeated oral administrations of S-312-d, a novel calcium channel antagonist, on tonic convulsions in spontaneously epileptic rats., J. Pharmacol. Sci., 95(3), 355-362, 2004
  77. Propagation of gammaPKC translocation along the dendrites of Purkinje cell in gammaPKC-GFP transgenic mice., Genes to Cells, 9(10), 945-957, 2004
  78. Involvement of alpha7- and alpha4beta2-type postsynaptic nicotinic acetylcholine receptors in nicotine-induced excitation of dopaminergic neurons in the substantia nigra: a patch clamp and single-cell PCR study using acutely dissociated nigral neurons., Mol. Brain Res., 129(1-2), 1-7, 2004
  79. Role of C-terminal region in the functional regulation of rat serotonin transporter (SERT)., Neurochem. Int, 46(2), 93-105, 2005
  80. Phosphorylation of PKC activation loop plays an important role in receptor-mediated translocation of PKC., Genes to Cells, 10(3), 225-239, 2005
  81. Mutant protein kinase Cgamma found in spinocerebellar ataxia type 14 is susceptible to aggregation and causes cell death., J. Biol. Chem, 280(32), 29096-29106, 2005
  82. Microglial alpha7 nicotinic acetylcholine receptors drive a phospholipase C/IP3 pathway and modulate the cell activation toward a neuroprotective role., J. Neurosci Res., 83(8), 1461-1470, 2006
  83. Identification of a new family of spinocerebellar ataxia type 14 in the Japanese spinocerebellar ataxia population by the screening of PRKCG exon 4., Movement Disorders, 21(9), 1355-1360, 2006
  84. Embryonic stem cell-derived neuron models of Parkinson's disease exhibit delayed neuronal death., J. Neurochem., 98(1), 45-56, 2006
  85. R659S mutation of gammaPKC is susceptible to cell death: implication of this mutation/polymorphism in the pathogenesis of retinitis pigmentosa., Neurochem. Int., 49(7), 669-675, 2006
  86. Bone marrow stromal cells promoting corticospinal axon growth through the release of humoral factors in organotypic cocultures in neonatal rats., J. Neurosurg Spine, 6(5), 412-419, 2007
  87. Fragmentation of protein kinase N (PKN) in the hydrocephalic rat brain., Acta Histochem.Cytochem., 40(4), 113-121, 2007
  88. Magnetically labeled neural progenitor cells, which are localized by magnetic force, promote axon growth in organotypic cocultures., Spine, 32(21), 2320-2305, 2007
  89. Aggregate formation of mutant protein kinase C gamma found in spinocerebellar ataxia type 14 impairs ubiquitin-proteasome system and induces endoplasmic reticulum stress., Eur. J. Neurosci., 26(11), 3126-3140, 2007
  90. Mutant gammaPKC found in spinocerebellar ataxia type 14 induces aggregate-independent maldevelopment of dendrites in primary cultured Purkinje cells., Neurobiol. Dis., 33(2), 260-273, 2009
  91. The C-terminal region of serotonin transporter is important for its trafficking and glycosylation., J. Pharmacol. Sci, 111(4), 392-404, 2009
  92. Mutant protein kinase C gamma that causes spinocerebellar ataxia type 14 (SCA14) is selectively degraded by autophagy., Genes to Cells, 15(5), 425-437, 2010
  93. A critical role of conventional protein kinase C in morphological changes of rodent mast cells., Immunol. Cell Biol., 89(1), 149-159, 2011
  94. Effect of trehalose on the properties of mutant {gamma}PKC, which causes spinocerebellar ataxia type 14, in neuronal cell lines and cultured Purkinje cells., J Biol. Chem., 285(43), 33252-33264, 2010
  95. Congo red, an amyloid-inhibiting compound, alleviates various types of cellular dysfunction triggered by mutant protein kinase cγ that causes spinocerebellar ataxia type 14 (SCA14) by inhibiting oligomerization and aggregation., J. Pharmacol. Sci., 114(2), 33252-33264, 2010
  96. Extracellular ATP differentially modulates Toll-like receptor 4-mediated cell survival and death of microglia., J. Neurochem., 116(6), 1138-1147, 2011
  97. Elucidation of the molecular mechanism and exploration of novel therapeutics for spinocerebellar ataxia caused by mutant protein kinase Cγ., J. Pharmacol. Sci., 116(3), 239-247, 2011
  98. Molecular pathophysiology of neurodegenerative disease caused by γPKC mutations., World J. Biol. Psychiatry, 12 Suppl 1, 95-98, 2011
  99. Hypoxic stress activates chaperone-mediated autophagy and modulates neuronal cell survival., Neurochem. Int, 60(4), 431-442, 2012
  100. Establishment of a novel fluorescence-based method to evaluate chaperone-mediated autophagy in a single neuron., PLoS ONE, 7(2), e31232, 2012
  101. Long-term exposure of RN46A cells expressing serotonin transporter (SERT) to a cAMP analog up-regulates SERT activity and is accompanied by neural differentiation of the cells., J. Pharmacol. Sci., 121(1), 25-38, 2013
  102. Effects of the chemical chaperone 4-phenylbutylate on the function of the serotonin transporter (SERT) expressed in COS-7 cells., J. Pharmacol. Sci., 122(2), 71-83, 2013
  103. Principles for the use of in vivo transgene techniques: overview and an introductory practical guide for the selection of tetracycline-controlled transgenic mice., Methods Mol Biol., 1142, 2014
  104. Deregulation of the actin cytoskeleton and macropinocytosis in response to phorbol ester by the mutant protein kinase C gamma that causes spinocerebellar ataxia type 14., Frontiers in Physiology, 5, 2014
  105. Developmental expression of GPR3 in rodent cerebellar granule neurons is associated with cell survival and protects neurons from various apoptotic stimuli., Neurobiol. Dis., 68, 2014
  106. Selective blockade of N-methyl-D-aspartate channels in combination with dopamine receptor antagonism induces loss of the righting reflex in mice, but not immobility., Psychopharmacology, 232(1), 2015
  107. The Subcellular Dynamics of the Gs-Linked Receptor GPR3 Contribute to the Local Activation of PKA in Cerebellar Granular Neurons, PLOS ONE, 11(1), 20160122
    Link to Paper Search Results by DOI
  108. The Toll-like receptor 4-activated neuroprotective microglia subpopulation survives via granulocyte macrophage colony-stimulating factor and JAK2/STAT5 signaling, NEUROCHEMISTRY INTERNATIONAL, 93, 82-94, 201602
    Link to Paper Search Results by DOI
  109. The Development of Screening Methods to Identify Drugs to Limit ER Stress Using Wild-type and Mutant Serotonin Transporter, ACTA HISTOCHEMICA ET CYTOCHEMICA, 49(6), 197-206, 2016
    Link to Paper Search Results by DOI
  110. Modifications of tau protein after cerebral ischemia and reperfusion in rats are similar to those occurring in Alzheimer's disease - Hyperphosphorylation and cleavage of 4-and 3-repeat tau, JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM, 37(7), 2441-2457, 201707
    Link to Paper Search Results by DOI
  111. Yokukansan enhances the proliferation of B65 neuroblastoma., J. Traditional Complement. Med., 7, 34-44, 2017
  112. Validation of Anti-CSP alpha, SNAP25, Tyrosine Hydroxylase, Ubiquitin, Cleaved Caspase 3, and pSer PKC Motif Antibodies for Utilization in Western Blotting, ACTA HISTOCHEMICA ET CYTOCHEMICA, 50(6), 177-180, 2017
    Link to Paper Search Results by DOI
  113. The Role of Cysteine String Protein alpha Phosphorylation at Serine 10 and 34 by Protein Kinase C gamma for Presynaptic Maintenance, JOURNAL OF NEUROSCIENCE, 38(2), 278-290, 20180110
    Link to Paper Search Results by DOI
  114. Propofol induced diverse and subtype-specific translocation of PKC families, JOURNAL OF PHARMACOLOGICAL SCIENCES, 137(1), 20-29, 201805
    Link to Paper Search Results by DOI
  115. Mutant γPKC that causes spinocerebellar ataxia type 14 upregulates Hsp70, which protects cells from the mutant's cytotoxicity., Biochem. Biophys. Res. Commun, 440, 25-30, 2013
  116. Pharmacological induction of heat shock proteins ameliorates toxicity of mutant PKC gamma in spinocerebellar ataxia type 14, JOURNAL OF BIOLOGICAL CHEMISTRY, 293(38), 14758-14774, 20180921
    Link to Paper Search Results by DOI
  117. SKF-10047, a prototype Sigma-1 receptor agonist, augmented the membrane trafficking and uptake activity of the serotonin transporter and its C-terminus-deleted mutant via a Sigma-1 receptor-independent mechanism, JOURNAL OF PHARMACOLOGICAL SCIENCES, 139(1), 29-36, 201901
    Link to Paper Search Results by DOI
  118. Spinocerebellar ataxia type 14 caused by a nonsense mutation in the PRKCG gene, MOLECULAR AND CELLULAR NEUROSCIENCE, 98, 46-53, 201907
    Link to Paper Search Results by DOI
  119. Histone deacetylase 10 knockout activates chaperone-mediated autophagy and accelerates the decomposition of its substrate, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 523(1), 246-252, 20200226
    Link to Paper Search Results by DOI
  120. Propofol induces the elevation of intracellular calcium via morphological changes in intracellular organelles, including the endoplasmic reticulum and mitochondria, EUROPEAN JOURNAL OF PHARMACOLOGY, 884, 20201005
    Link to Paper Search Results by DOI
  121. The indirect gamma-aminobutyric acid (GABA) receptor agonist gabaculine-induced loss of the righting reflex may inhibit the descending analgesic pathway, PHARMACOLOGY BIOCHEMISTRY AND BEHAVIOR, 198, 202011
    Link to Paper Search Results by DOI
  122. Detailed neuronal distribution of GPR3 and its co-expression with EF-hand calcium-binding proteins in the mouse central nervous system, BRAIN RESEARCH, 1750, 20210101
    Link to Paper Search Results by DOI
  123. Role of the E3 ubiquitin ligase HRD1 in the regulation of serotonin transporter function, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 534, 583-589, 20210101
    Link to Paper Search Results by DOI
  124. Component of nicotine-induced intracellular calcium elevation mediated through alpha 3-and alpha 5-containing nicotinic acetylcholine receptors are regulated by cyclic AMP in SH-SY 5Y cells, PLOS ONE, 15(11), 20201130
    Link to Paper Search Results by DOI
  125. Syntaxin 3 interacts with serotonin transporter and regulates its function, JOURNAL OF PHARMACOLOGICAL SCIENCES, 145(4), 297-307, 202104
    Link to Paper Search Results by DOI
  126. Evaluation of the Effectiveness of Post-Stroke Metformin Treatment Using Permanent Middle Cerebral Artery Occlusion in Rats, PHARMACEUTICALS, 14(4), 202104
    Link to Paper Search Results by DOI
  127. Effects of flurbiprofen on the functional regulation of serotonin transporter and its misfolded mutant, JOURNAL OF PHARMACOLOGICAL SCIENCES, 148(1), 187-195, 202201
    Link to Paper Search Results by DOI
  128. Pentobarbital may protect against neurogenic inflammation after surgery via inhibition of substance P release from peripheral nerves of rats, NEUROSCIENCE LETTERS, 771, 20220206
    Link to Paper Search Results by DOI
  129. GPR3 accelerates neurite outgrowth and neuronal polarity formation via PI3 kinase-mediating signaling pathway in cultured primary neurons, MOLECULAR AND CELLULAR NEUROSCIENCE, 118, 202201
    Link to Paper Search Results by DOI

Invited Lecture, Oral Presentation, Poster Presentation

  1. Application of G protein-coupled receptor 3 for axonal regeneration therapy in the retina using an adeno-associated virus vector, 2022/11/30, Without Invitation, Japanese
  2. OpTER: a low-cost method for measuring the transepithelial/endothelial electrical resistance TER of cell layers, which reduces research costs and the burden on us all, 2022/11/30, Without Invitation, Japanese
  3. NECAB2 is a major calcium-binding protein of GPR3-positive neurons in various regions of the mouse brain., F. IKAWA, S. TANAKA, K. HARADA, I. HIDE, N. SAKAI, Neuroscience 2019, 2019/10/23, Without Invitation, English, Society for Neuroscience, Chicago, USA
  4. The mechanism underlying the propofol-induced elevation of intracellular calcium, T. URABE, N. SAKAI, S. MOTOIKE, K. HARADA, I. HIDE, S. TANAKA, Neuroscience 2019, 2019/10/23, Without Invitation, English, Society for Neuroscience, Chicago, USA
  5. GPR3 is upregulated in rodent mast cells immediately after brain ischemia and modulates degranulation., S. TANAKA, Y. HAMAKAWA, Y. YANASE, M. YAMAMOTO, H. SHIRAKI, K. HARADA, I. HIDE, N. SAKAI, Neuroscience 2019, 2019/10/20, Without Invitation, English, Society for Neuroscience, Chicago, USA
  6. The potential role of G protein-coupled receptor 3 in mast cell degranulation following brain ischemia., Shigeru Tanaka, Yuhki Yanase, Yuki Hamakawa, Masahiro Yamamoto, Hiroko Shiraki, Kana Harada, Izumi Hide, Norio Sakai, 2019/07/26, Without Invitation, English
  7. Expression and function of P2Y2 and P2Y13 receptors in purinergic and Toll-like receptor 4-activated microglia, Izumi Hide, Yoichiro Morita, Hiroko Shiraki, Yuhki Yanase, Kana Harada, Shigeru Tanaka, Norio Sakai, 2019/07/26, Without Invitation, English
  8. Differentiation-induced G protein-coupled receptor 3 modulates phosphorylation of synapsin in PC12 cells., Humiaki Ikawa, Shigeru Tanaka, Kenta Sasaki, Tomohiro Noguchi, Kana Harada, Izumi Hide, Norio Sakai, 2019/07/26, Without Invitation, English
  9. Intrinsic expression of G protein-coupled receptor 3 facilitates formation of neuronal polarity in hippocampal neurons., Shigeru Tanaka, Naoto Shimada, Masahiro Yamamoto, Tatsuhiro Miyagi, Hiroko, Shiraki, Isumi Hide Norio Sakai, Neuroscience meeting 2018, 2018/11/04, Without Invitation, English, Society for Neuroscience, San Diego
  10. SKF-10047, a prototype Sigma-1 receptor agonist, facilitated the membrane trafficking and uptake activity of serotonin transporter and its mutant through thesigma-1 receptor-independent mechanism., Masaya Asano, Hikaru Yamamoto, Izumi Hide, Shigeru Tanaka, Norio Sakai, Neuroscience meeting 2018, 2018/11/04, Without Invitation, English, Society for Neuroscience, San Diego
  11. SKF-10047, a prototype Sigma-1 receptor agonist, facilitated the membrane trafficking and uptake activity of serotonin transporter and its mutant by the mechanism independent of Sigma-1receptor, Norio Sakai, Masaya Asano, Chika Yokota, Naoto Usuki, Hikaru Yamamoto, Izumi Hide, Shigeru Tanaka, 2018/09/08, Without Invitation, Japanese
  12. Potential role of G-protein-coupled receptor 3 in mast cells following brain ischemia., Shigeru Tanaka, Yuhki Yanase, Masahiro Yamamoto, Tatsuhiro Miyagi, Hiroko, Shiraki, Izumi Hide, Norio Sakai, 2018/07/03, Without Invitation, English
  13. SKF-10047, a prototype Sigma-1 receptor agonist, accelerated the membrane trafficking and uptake activity of serotonin transporter and its mutant via the mechanism independent of Sigma-1receptor., Norio Sakai, Masaya Asano, Chika Yokota, Naoto Usuki, Hikaru Yamamoto, Izumi Hide, Shigeru Tanaka, 2018/07/02, Without Invitation, English
  14. Purinergic receptor is involved in dying cell phagocytosisi and mediator production in Toll-like receptor 4-activated microglia, Izumi Hide, Hiroko Shiraki,, Yuhki Yanase, Toshihiko Shirafuji, Shigeru, Tanaka, Norio Sakai, 2018/07/02, Without Invitation, English
  15. The potential role of G-protein-coupled receptor 3 in the formation of neuronal polarity in rat hippocampal neurons, S. TANAKA, N. SHIMADA, T. MIYAGI, I. HIDE, T. SHIRAFUJI, N. SAKAI, Neuroscience meeting, 2017/11/12, Without Invitation, English
  16. The development of screening methods to identify drugs, which can relieve ER stress, using wild-type and mutant serotonin transporter, 2017/09/08, Without Invitation, English
  17. PKCgamma knockout Parkinson syndrome model: The role of PKCgamma for Parkinsonian symptoms, 2016/05/20, Without Invitation, Japanese
  18. Analyses of signaling pathway involved in the GPR3-mediated neurite outgrowth., Tanaka, S. Miyagi, T., Hide, I., Shirafuji, T.and Sakai, N., Neuroscience meeting 2015, 2015/10/17, Without Invitation, English
  19. Neuronal expression of GPR3 in rodent brain is associated with cell survival, Tanaka S, Miyagi T, Sakai N. et.al, 2014/11/19, Without Invitation, English
  20. The subcellular localization and local function of Gs-linked receptor GPR3 in neuronal cells, Miyagi T, Tanaka S, Sakai N et.al, Neuroscience meeting 2014　, 2014/11/18, Without Invitation, English
  21. The role of Cysteine string protein alpha (CSPa) phosphorylation at Ser10 and Ser34 by PKCg for the presynaptic maintenance,, Shirafuji, T., Ueyama, T., Adachi, N., Yoshino, K., Tanaka, S., Hide, I., Saito, N., Sakai, N., 20170917, Without Invitation, Japanese

Patented

  1. Patent

External Funds

Acceptance Results of Competitive Funds

  1. 2023, 2025
  2. 2023, 2025
  3. 2006
  4. 2003
  5. 2007
  6. 2003
  7. 2007
  8. 1999
  9. 2000
  10. 2007
  11. 2008
  12. 2009
  13. 2012, 2014
  14. KAKENHI, 2013, 2015
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  15. KAKENHI, 2012, 2014
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  16. KAKENHI, Development of screening methods to find novel therapeutic drugs for neurodegenerative disease using fluorescence-based probe and high-content microscope., 2012, 2013
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  17. KAKENHI, Development of therapeutic strategy for intractable neuropsychiatric disease using chemical chaperones, 2010, 2012
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  18. KAKENHI, Novel signaling pathways and anti-inflammatory roles of. 7 nicotinic acetylcholine receptors in microglia, 2009, 2011
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  19. KAKENHI, 2006, 2007
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  20. KAKENHI, Molecular characterization of mutant protein kinase C gamma found in spinocerebellar ataxia type 14 and its application to screening of therapeutics, 2006, 2007
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  21. KAKENHI, Functional analysis of protein kinase C(PKC), a molecule involved in plasticity, development and cell death in central nervous system, using gene-manipulated mice., 2004, 2005
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  22. KAKENHI, 2004, 2004
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  23. KAKENHI, Roles of protein kinase C (PKC) in the formation of neural plasticity. -Investigation using GFP-tagged PKC, 2002, 2003
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  24. KAKENHI, 2001, 2002
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  25. KAKENHI, Spatio-temporal signaling mechanism of protein kinase C, 2001, 2002
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  26. KAKENHI, 2000, 2001
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  27. KAKENHI, 2000, 2000
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  28. KAKENHI, Studies on neural plasticity using transgenic mice expressing GFP-tagged PKC, 2000, 2001
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  29. KAKENHI, 1999, 1999
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  30. KAKENHI, Tyrosine phosphorylation and activation of PKC and its role in celluar regulation, 1998, 1999
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  31. KAKENHI, Research for modulation of expression of serotonin transporter and its involvement in mental disorder, 1997, 1998
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  32. KAKENHI, 1997, 1997
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  33. KAKENHI, 1997, 1997
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  34. KAKENHI, 1996, 1996
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  35. KAKENHI, 1995, 1995
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  36. KAKENHI, 2016, 2018
  37. KAKENHI, 2016, 2017
  38. KAKENHI, 2015, 2018
  39. KAKENHI, 2015
  40. KAKENHI(Grant-in-Aid for Scientific Research (B)), 2019, 2021

Social Activities

Organizing Academic Conferences, etc.

  1. 第１３３回日本薬理学会近畿部会, 1806/01, 1806/01
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History as Peer Reviews of Academic Papers

  1. 2023, Hiroshima JOurnal of Medical Sciences, Chief editor
  2. 2015, Hiroshima Journal of Medical Sciences, Chief editor
  3. 2016, Hiroshima Journal of Medical Sciences, Chief editor
  4. 2017, Hiroshima Journal of Medical Sciences, Chief editor
  5. 2016, Journal of Pharmacological Sciences, Others
  6. 2017, Journal of Pharmacological Sciences, Others
  7. 2018, Hiroshima Journal of Medical Sciences, Chief editor
  8. 2019, Hiroshima Journal of Medical Sciences, Chief editor
  9. 2020, Hiroshima Journal of Medical Sciences, Chief editor
  10. 2021, Hiroshima Journal of Medical Sciences, Chief editor
  11. 2022, Hiroshima Journal of Medical Sciences, Chief editor