YOSHIKO OKAMURA

Last Updated :2024/06/04

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Professor
E-mail
okamurayhiroshima-u.ac.jp
Self-introduction
Focusing on the diversity and high functionality of marine bacteria, we have developed innovative gene detection and a novel gene synthesis technologies for the utilization of their useful genes. We are also producing biogenic metal crystals and biomass plastics from wastewater thorough their biological process.

Basic Information

Major Professional Backgrounds

  • 2011/01/01, 2019/03/31, Hiroshima University, Graduate School of Advanced Sciences of Matter, Associate Professor
  • 2019/04/01, 2020/03/31, Hiroshima University, Graduate School of Integrated Sciences for Life, Associate Professor
  • 2020/04/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Professor

Academic Degrees

  • Ph.D. in Engineering, Tokyo University of Agriculture and Technology
  • Master of Education, Tokyo Gakugei University

Educational Activity

  • [Bachelor Degree Program] School of Engineering : Cluster 3(Applied Chemistry, Biotechnology and Chemical Engineering) : Program of Biotechnology
  • [Master's Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology
  • [Doctoral Program] Graduate School of Integrated Sciences for Life : Division of Integrated Sciences for Life : Program of Biotechnology

In Charge of Primary Major Programs

  • Biotechnology

Research Fields

  • Chemistry;Applied chemistry;Energy-related chemistry
  • Chemistry;Applied chemistry;Bio-related chemistry
  • Engineering;Process / Chemical engineering;Biofunction / Bioprocess
  • Biological Sciences;Genome science;Genome biology

Research Keywords

  • biopolymer
  • Marine Biotechnology
  • Biomineralization
  • microbial genome
  • metagenome
  • Photosynthetic bacteria

Affiliated Academic Societies

  • Material Research Society
  • The Chemical Society of Japan
  • The Society for Biotechnology, Japan
  • The Molecular Biology Society of Japan
  • Japanese Society for Marine Biotechnology

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, First Semester, Training of Biotechnology I
  2. 2024, Undergraduate Education, 4Term, Genetic and protein engineering
  3. 2024, Undergraduate Education, Year, Graduation Thesis
  4. 2024, Graduate Education (Master's Program) , 2Term, societal implementation of life science
  5. 2024, Graduate Education (Master's Program) , Academic Year, Seminar in Biotechnology
  6. 2024, Graduate Education (Master's Program) , 1Term, Exercises in Biotechnology A
  7. 2024, Graduate Education (Master's Program) , 2Term, Exercises in Biotechnology A
  8. 2024, Graduate Education (Master's Program) , 3Term, Exercises in Biotechnology B
  9. 2024, Graduate Education (Master's Program) , 4Term, Exercises in Biotechnology B
  10. 2024, Graduate Education (Master's Program) , Academic Year, Research for Academic Degree Dissertation in Biotechnology
  11. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences
  12. 2024, Graduate Education (Doctoral Program) , Academic Year, Research for Academic Degree Dissertation in Integrated Life Sciences

Research Activities

Academic Papers

  1. Nonribosomal Peptide Synthetase Specific Genome Amplification Using Rolling Circle Amplification for Targeted Gene Sequencing., International Journal of Molecular Sciences, 25, 5089, 20240509
  2. Characterization of Biogenic PbS Quantum Dots, INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 24(18), 202309
  3. Development of a transformation system for Nitratireductor sp., Marine Biotechnology, Open access, 20230203
  4. Visualization of Gene Reciprocity among Lactic Acid Bacteria in Yogurt by RNase H-Assisted Rolling Circle Amplification-Fluorescence In Situ Hybridization, MICROORGANISMS, 9(6), 202106
  5. Evaluation of marine sediments as microbial sources for methane production from brown algae under high salinity, BIORESOURCE TECHNOLOGY, 169, 362-366, 201410
  6. Value-added lipid production from brown seaweed biomass by two-stage fermentation using acetic acid bacterium and thraustochytrid, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 98(22), 9207-9216, 201411
  7. Behavior of organics in kelp during hydrothermal pretreatment: Fundamental characteristics and effect of salt, Journal of the Japan Institute of Energy, 93, 531-535, 2014
  8. Isolation and characterization of a GDSL esterase from the metagenome of a marine sponge-associated bacteria, Marine Biotechnology, 12, 395-402, 201011
  9. Improved methane production from brown algae under high salinity by fed-batch acclimation, BIORESOURCE TECHNOLOGY, 187, 275-281, 201507
  10. Whole genome sequence of Desulfovibrio magneticus strain RS-1 revealed common gene clusters in magnetotactic bacteria, Genome Research, 19, 1801-1808, 2009
  11. Cellular response to electrochemical killing process by applying a constant potential in synchronously cultured Saccharomyces cerevisiae, Electrochemistry, 76, 603-605, 2008
  12. Development of cell surface display system in a Magnetotactic bacterium, Magnetospirillum magneticum AMB-1, Applied and Environmental Microbiology, 74, 3342-3348, 2008
  13. Cytoplasmic ATPase involved in ferrous ion uptake from magnetotactic bacterium Magnetospirillum magneticum AMB-1, FEBS Letter, 581, 3443-3448, 2007
  14. Origin of Magnetosome Membrane: Proteomic Analysis of Magnetosome Membrane and Comparison with Cytoplasmic Membrane, Proteomics, 6, 5234-5247, 2006
  15. Global gene expression analysis of iron-inducible genes in Magnetospirillum magneticum AMB-1, Journal of Bacteriology, 188, 2275-2279, 2006
  16. Dynamic analysis of genomeic island in Magnetospirillum sp. strain AMB-1 reveals how magnetosome synthesis developed, FEBS Letter, 580, 801-812, 2006
  17. Complete genome sequence of the facultative anaerobic magnetotactic bacterium Magnetospirillum sp. strain AMB-1, DNA Research, 12, 157-166, 2005
  18. Specific degradation of H. pylori urease by a catalytic antibody light chain, FEBS Journal, 272, 4497-4505, 2005
  19. Siderophore Production of a Periplasmic Transport Bionding Protein Kinase Gene Defective Mutant of Magnetospirillum magneticum AMB-1, Biochemical and Biophysical Research Communication, 323, 852-857, 2004
  20. Assembly of G protein-coupled receptors onto nanosized bacterial magnetic particles using Mms16 as an anchor molecule, Applied and Environmental Microbiology, 70, 2880-2885, 2004
  21. Biotechnological Application of Nano-Scale Engineered Bacterial Magnetic Particles, Journal of Materials Chemistry, 14, 2099-2105, 2004
  22. Design and application of a new cryptic-plasmid-based shuttle vector for Magnetospirillum magneticum, Applied and Environmental Microbiology, 69, 4274-4277, 2003
  23. Characterization of aldehyde ferredoxin oxidoreductase gene defective mutant in Magnetospirillum magneticum AMB-1, Biochemical and Biophysical Research Communication, 303, 223-229, 2003
  24. Siderophore production by the magnetic bacterium Magnetospirillum magneticum AMB-1., FEMS Microbiology Letter, 218, 371-375, 2003
  25. Genes and proteins involved in bacterial magnetic particle formation, Trends in Microbiology, 11(11), 536-541, 2003
  26. MAGNETIC BACTERIA AND THEIR APPLICATIONS, Biomolecular Engineering, 20(2), 49-49, 2003
  27. Bionanomagnet and its application, International Journal of Nanoscience, 1(5 & 6), 1-7, 2002
  28. Molecular mechanism of bacterial magnetite formation and its application, Material Research Society Symposium Proceeding, 724, N.1.4.1., 2002
  29. A magnetosome specific GTPase from the magnetic bacterium Magnetospirillum magneticum AMB-1, Journal of Biological Chemistry, 276, 48183-48188, 2001
  30. Cloning and characterization of a gene, mpsA, encoding a protein associated with intracellular magnetic particles from Magnetospirillum sp. strain AMB-1, Biochemical and Biophysical Research Communication, 268, 932-937, 2000
  31. Two-dimensional analysis of proteins specific to the bacterial magnetic particle membrane from Magnetospirillum sp. AMB-1, Applied Biochemistry and Biotechnology, 84-86, 441-446, 2000
  32. Identification of the gene cluster involved in ferredoxin in the flanking region of transposon from non-magnetic mutant of Magnetospirillum sp. AMB-1, Proceeding of "8th INTERNATIONAL CONFERENCE ON FERRITES, 84-86, 2000
  33. Determination of Mannitol Decomposition Rate under Hydrothermal Pretreatment Condition, JOURNAL OF THE JAPAN PETROLEUM INSTITUTE, 58(4), 252-255, 201507
  34. Selenium recovery and conversion by a Filamentous Fungus, Aspergillus oryzae Strain RIB40, Asia Pacific Journal of Sustainable Agriculture Food and Energy, 2(2), 5-8, 2014
  35. Effect of Salinity on Methanogenic Propionate Degradation by Acclimated Marine Sediment-Derived Culture, APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY, 177(7), 1541-1552, 201512
  36. Semi-continuous methane production from undiluted brown algae using a halophilic marine microbial community, BIORESOURCE TECHNOLOGY, 200, 616-623, 201601
  37. Dysgonomonas alginatilytica sp nov., an alginate-degrading bacterium isolated from a microbial consortium, INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 65, 3570-3575, 201510
  38. Isolation and characterization of bacterium producing lipid from short-chain fatty acids, BIORESOURCE TECHNOLOGY, 201, 215-221, 201602
  39. High-rate Fermentation of Acetate to Methane under Saline Condition by Aceticlastic Methanogens Immobilized in Marine Sediment, PEDIATRIC NEUROLOGY, 55, 9-15, 201602
  40. Characterization of a halotolerant acetoclastic methanogen highly enriched from marine sediment and its application in removal of acetate, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(2), 196-202, 201602
  41. Bacterial community structure and predicted alginate metabolic pathway in an alginate-degrading bacterial consortium, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 121(3), 286-292, 201603
  42. Development of a bench-top extra-cleanroom for DNA amplification., Bio Techniques, 61(1), 42-46, 201607
  43. Decomposition kinetics of uronic acids obtained from kelp under hydrothermal condition, Journal of the Energy Institute, 90, 185-190, 2017
  44. Decomposition kinetics of mannose, its sugar alcohol, and its uronic acid under hydrothermal condition., Journal of Chemical Engineering of Japan, 49, 663-667, 2016
  45. Characterization of a novel gene involved in cadmium accumulation screened from sponge-associated bacterial metagenome., Gene, 576, 618-625, 2016
  46. Role of carbonic anhydrase in photosynthesis of unicellular marine algae coccolithophorids (Haptophyta)., Research in photosynthesis, 3, 783-786, 1992
  47. Molecular mechanism of magnetosome formation in Magnetospirillum magneticum AMB-1, BIOMINERALIZATION (BIOM2001) formation, diversity, evolution and application, 272-276, 2002
  48. Development of a bench-top extra-cleanroom for DNA amplification, BIOTECHNIQUES, 61(1), 42-46, 201607
  49. Kinetics of Sorbitol Decomposition under Hydrothermal Condition, JOURNAL OF THE JAPAN PETROLEUM INSTITUTE, 59(4), 149-154, 201607
  50. Decomposition Kinetics of Mannose, Its Sugar Alcohol, and Its Uronic Acid under Hydrothermal Condition, JOURNAL OF CHEMICAL ENGINEERING OF JAPAN, 49(7), 663-667, 201607
  51. Kinetics of sorbitol decomposition under hydrothermal condition, Journal of Japan Petroleum Institute, 59, 149-154, 2016
  52. In-situ mass spectroscopic analysis of glucose decomposition under hydrothermal condition: Quantitative analysis for reaction kinetics, Journal of the Japan Petroleum Institute, 60, 101-109, 2017
  53. In situ mass spectrometry of glucose decomposition under hydrothermal reactions, KOREAN JOURNAL OF CHEMICAL ENGINEERING, 34(5), 1524-1530, 201705
  54. Real-Time Mass Spectrometric Analysis of Hydrothermal Reaction Products, INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH, 56(36), 9993-9998, 20170913
  55. Improved methanization and microbial diversity during batch mode cultivation with repetition of substrate addition using defined organic matter and marine sediment inoculum at seawater salinity, BIORESOURCE TECHNOLOGY, 245, 833-840, 201712
  56. Quantitative In Situ Mass Spectrometry Analysis of Mannitol Decomposition Products under Hydrothermal Conditions, ENERGY & FUELS, 31(10), 10866-10873, 201710
  57. Efficient conversion of mannitol derived from brown seaweed to fructose for fermentation with a thraustochytrid, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 125(2), 180-184, 201802
  58. Isolation of High Carotenoid-producing Aurantiochytrium sp Mutants and Improvement of Astaxanthin Productivity Using Metabolic Information, JOURNAL OF OLEO SCIENCE, 67(5), 571-578, 201805
  59. ★, RNase H-assisted RNA-primed rolling circle amplification for targeted RNA sequence detection, SCIENTIFIC REPORTS, 8, 7770, 20180517
  60. Biomineralization of metallic tellurium by bacteria isolated from marine sediment off Niigata Japan, Proceedings of BIOMIN XIV, 2018
  61. Potential of Nitratireductor sp. OM-1 to produce more short-chain esters and less excess sludge during wastewater treatment, Papers of the 26th European Biomass Conference, 917-920, 201805
  62. Metabolite Profile Analysis of Aurantiochytrium limacinum SR21 Grown on Acetate-based Medium for Lipid Fermentation, JOURNAL OF OLEO SCIENCE, 68(6), 541-549, 201906
  63. RNA direct detection through rolling circle amplification, THE CHEMICAL TIMES, 251(1), 12-17, 201901
  64. ★, Discovery of a novel gene conferring tellurite tolerance through tellurite reduction to Escherichia coli transformant in marine sediment metagenomic library, Marine Biotechnology, 21(6), 762-772, 201912
  65. ★, Complete genome sequence of Nitratireductor sp. strain OM-1: A lipid-producing bacterium with potential use in wastewater treatment, Biotechnology Reports, 24, e00366, 201912
  66. ★, Direct detection of mRNA expression in microbial cells by fluorescence in situ hybridization using RNase H-assisted rolling circle amplification, SCIENTIFIC REPORTS, 10(1), 20200615
  67. Improvement of fatty acid productivity of thraustochytrid, Aurantiochytrium sp. by genome editing, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 131(4), 373-380, 20210401
  68. Screening of Neutrophil Activating Factors from a Metagenome Library of Sponge-Associated Bacteria, MARINE DRUGS, 19(8), 202108

Publications such as books

  1. 2018/04, Biomineralization From Molecular and Nano-structural Analyses to Environmental Science, Chapter 31. Biomineralization of metallic tellurium by bacteria isolated from marine sediment off Niigata Japan., Springer, 2018, 2018, Scholarly Book, Joint work, English, Madison Pascual Munar, Tadaaki Matsuo, Hiromi Kimura, Hirokazu Takahashi, Yoshiko Okamura, 978-981-13-1001-0, 413, 291-301

Invited Lecture, Oral Presentation, Poster Presentation

  1. Biofuel production by genetic engineering in Nitratireductor sp. OM-1 and Escherichia coli, Hiroto Maeda, Yuto Hirata, Hirokazu Takahashi, Kenshi Watanabe, Tsunehiro Aki, Yoshiko Okamura, Hiroto Maeda, Yuto Hirata, Hirokazu Takahashi, Kenshi Watanabe, Tsunehiro Aki, Yoshiko Okamura, International Symposium on Fuel & Energy 2022, 2022/07/05, Without Invitation, English, Advanced Core for Energetics Hiroshima University (HU-ACE), Online
  2. Thin film crystalline and spherical nanocrystalline biogenic PbS, Takumi Konishi, Tomoya Murakami, Yoshiko Okamura, Yoriko Tominaga, Takumi Konishi, Tomoya Murakami, Yoshiko Okamura, Yoriko Tominaga, MRS Spring meeting 2022, 2022/05/10, Without Invitation, English, Material Research Society, Hawaii, USA
  3. Biogenic Nanoparticles – The morphology is controlled even by BIM (biological induced mineralization), Yoshiko Okamura, MRS Spring meeting 2022, 2022/05/10, With Invitation, English, Material Research Society, Hawaii, USA
  4. Ultrafine nanoparticles of PbS synthesized by biologically induced mineralization, Yoshiko Okamura, Pacifichem2021, 2021/12/19, Without Invitation, English, Online
  5. What would you produce in wastewater? Products by photosynthetic bacteria in marine biomass waste , Yoshiko Okamura, The 2nd Japan-Hawaii Joint Workshop, 2014/11/06, Higashi-Hiroshima
  6. Oil production from organic acids derived from marine algal biomass by Nitratireductor sp. OM-1., Yoshiko Okamura, Shota Nakai, Tsunehiro Aki, Yutaka Nakashimada, Yukihiko Matsumura, Takahisa Tajima., APMBC 2017, 2017/05/23, Without Invitation, English, Asia-Pacific Marine Biotechnology conference, Hawaii, USA
  7. Potential of Nitratireductor sp. OM-1 to produce more short-chain esters and less excess sludge during wastewater treatment., Yoshiko Okamura, EUBCE 2018, 2018/05/14, Without Invitation, English, European Biomass Conference & Exibition, Copenhagen, Denmark
  8. Removal of ammonium, inhibitor of biodiesel production in Nitratireductor sp. OM – 1, toward wastewater utilization, Yusuke Noboru, Hirokazu Takahashi, Tsunehiro Aki, Yutaka Nakashimada, Yukihiko Matsumura, Yifeng Zhang, Irini Angelidaki, Yosiko Okamura, International Symposium on Fuel and Energy 2018, 2018/06/06, Without Invitation, English
  9. Characteristics of biogenic PbS towards future fabrication of biogenic semiconductor devices, Yoriko Tominaga, Ryo Shimizu, Sachiko Maki, Makoto Maeda, Yoshiko Okamura, The 3rd International Symposium on Biomedical Engineering, 2018/11/08, Without Invitation, English, Hiroshima, Japan
  10. Characteristics of biogenic sulfide semiconductor nanomaterials, Yoriko Tominaga, Ryo Shimizu, Sachiko Maki, Makoto Maeda, Yoshiko Okamura, Material Research Society Fall Meeting 2018, 2018/11/26, Without Invitation, English, Material Research Society, Boston MA, USA
  11. Challenges for Biodiesel Fuel Production from Wastewater with Nitratireductor sp. OM-1, Yoshiko Okamura, 2018 Japan-Denmark Workshop on Biomass Technology, 2019/03/04, With Invitation, English, Gifu Renewable Energy System Research Center Organization for Research and Community Development Gifu University, Gifu, Japan
  12. Biogenic nanoparticles – Potential as a semiconductor, Yoshiko Okamura, The 26th Assembly of Advanced Materials Congress, 2019/06/12, With Invitation, English, Stockholm, Sweden

Awards

  1. 2019/09/13, MBC2019 Student Poster Award, Marine Biotechnology Conference 2019 Chair, Analysis of lipid synthesis pathway in short-chain ester producing bacterium Nitratireductor sp. OM-1
  2. 2019/11/20, MEWE2019 The Best Poster Award, MEWE2019 Conference Chair, in situ mRNA detection toward elucidation of functional consortium
  3. 2002, Young Investigator Award, Gordon Research Conference on Biomineralization conference 2002, Molecular Mechanism of Bacterial Magnetite Formation

Social Activities

History as Committee Members

  1. International Journal of Molecular Science Editorial Board Member, 2020/04, International Journal of Molecular Science
  2. Marine Biotechnology Editorial Board Member, 2019/10, Marine Biotechnology

Organizing Academic Conferences, etc.

  1. Marine Biotechnology Conference2019, Local committee, 2018/08, 2019/09