Takanori Kumagai

Last Updated :2023/02/02

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Associate Professor
E-mail
tkumahiroshima-u.ac.jp
Self-introduction
I carry out studies using some kind of microorganism, called 'Actinomycetes', known as producers of various beneficial secondary metabolites.

Basic Information

Major Professional Backgrounds

  • 1995/04/01, 2003/03/31, Department of Pharmaceutical Sciences, Hiroshima University, Research Associate
  • 2003/04/01, 2004/01/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Assistant Professor
  • 2004/02/01, 2007/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Associate professor
  • 2007/04/01, 2012/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Associate professor
  • 2012/04/01, 2019/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Associate professor
  • 2019/04/01, Graduate School of Biomedical and Health Sciences, Associate Professor

Academic Degrees

  • Master of Pharmaceutical Science, Hiroshima University
  • Doctor of Philosophy in Pharmaceutical Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Medicinal Sciences : Medicinal Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Natural medicines

Research Keywords

  • secondary metabolism
  • Genome analysis
  • heterologous production
  • antibiotic-producer
  • antibiotics

Affiliated Academic Societies

  • The Society for Actinomycetes Japan, 1994/04
  • The Society for Biotechnology Japan, 1995/04
  • The Japanese Biochemical Society, 2005/04
  • The Pharmaceutical Society of Japan, 2005/04
  • Japanese Society for Bacteriology, 2018/02

Educational Activity

Course in Charge

  1. 2022, Undergraduate Education, 4Term, Biochemistry II
  2. 2022, Undergraduate Education, First Semester, Practice of Microbiology
  3. 2022, Undergraduate Education, 3Term, Genetic Engineering
  4. 2022, Undergraduate Education, Intensive, Genetic Engineering
  5. 2022, Undergraduate Education, First Semester, Experiments of Microbial Chemistry
  6. 2022, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences I
  7. 2022, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences II
  8. 2022, Undergraduate Education, Second Semester, Special laboratory Works in Pharmaceutical Sciences III
  9. 2022, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy I
  10. 2022, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy II
  11. 2022, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy III
  12. 2022, Liberal Arts Education Program1, 1Term, Introductory Seminar for First-Year Students
  13. 2022, Graduate Education (Master's Program) , First Semester, Lecture on Medicinal Science
  14. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Lecture on Medicinal Science
  15. 2022, Graduate Education (Master's Program) , First Semester, Seminar on Microbiology
  16. 2022, Graduate Education (Master's Program) , Second Semester, Seminar on Microbiology
  17. 2022, Graduate Education (Master's Program) , First Semester, Research on Microbiology
  18. 2022, Graduate Education (Master's Program) , Second Semester, Research on Microbiology
  19. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Microbiology
  20. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Microbiology
  21. 2022, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Microbiology
  22. 2022, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Microbiology
  23. 2022, Graduate Education (Master's Program) , First Semester, Seminar on Antibiotic Biosynthesis
  24. 2022, Graduate Education (Master's Program) , Second Semester, Seminar on Antibiotic Biosynthesis

Research Activities

Academic Papers

  1. Genome sequence-guided finding of lucensomycin production by Streptomyces achromogenes subsp. streptozoticus NBRC14001, Microorganisms, 10(1), 37, 20211226
  2. Catalytic specificity of the Lactobacillus plantarum cystathionine gamma-lyase presumed by the crystallographic analysis, SCIENTIFIC REPORTS, 10(1), 14886, 20200910
  3. Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10, Scientific Reports, 9(1), 4854, 201903
  4. Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 84(7), e02707-e02717, 201804
  5. Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived Lactobacillus plantarum, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 41(4), 536-545, 201804
  6. Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 40(5), 621-629, 201705
  7. Antiobesity effect of Pediococcus pentosaceus LP28 on overweight subjects: a randomized, double-blind, placebo-controlled clinical trial, EUROPEAN JOURNAL OF CLINICAL NUTRITION, 70(5), 582-587, 201605
  8. Characterization of the LP28 starin-specific exopolysaccharide biosynthetic gene cluster found in the whole circular genome of Pediococcus pentosaceus, Biochemistry and Biophysics Reports, 5, 266-271, 2016
  9. Oral lactic acid bacteria that are related to occurrence and/or progression of dental caries in Japanese preschool children, Biosci. Microbiota Food Health, 34, 29-36, 2015
  10. The structural and mutational analyses of O-ureido-l-serine synthase necessary for d-cycloserine biosynthesis, FEBS JOURNAL, 282(20), 3929-3944, 201510
  11. ★, High-Level Heterologous Production of D-Cycloserine by Escherichia coli, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(22), 7881-7887, 201511
  12. Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 38(12), 1902-1909, 201512
  13. An alternative allosteric regulation mechanism of an acidophilic L-lactate dehydrogenase from Enterococcus mundtii 15-1A, FEBS OPEN BIO, 4, 834-847, 2014
  14. Aspergillus oryzae S-03 produces gingipain inhibitors as a virulence factor for Porphyromonas gingivalis, J. Bacteriol. Virol., 44, 152-161, 2014
  15. Establishment of an In Vitro D-Cycloserine-Synthesizing System by Using O-Ureido-L-Serine Synthase and D-Cycloserine Synthetase Found in the Biosynthetic Pathway, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 57(6), 2603-2612, 201306
  16. Crystallographic Study To Determine the Substrate Specificity of an L-Serine-Acetylating Enzyme Found in the D-Cycloserine Biosynthetic Pathway, JOURNAL OF BACTERIOLOGY, 195(8), 1741-1749, 201304
  17. Crystallographic and mutational analyses of tannase from Lactobacillus plantarum, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 81(11), 2052-2058, 201311
  18. The Obesity and Fatty Liver Are Reduced by Plant-Derived Pediococcus pentosaceus LP28 in High Fat Diet-Induced Obese Mice, PLOS ONE, 7(2), 20120217
  19. ★, Heme Protein and Hydroxyarginase Necessary for Biosynthesis of D-Cycloserine, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56(7), 3682-3689, 201207
  20. A Molecular Mechanism for Copper Transportation to Tyrosinase That Is Assisted by a Metallochaperone, Caddie Protein, JOURNAL OF BIOLOGICAL CHEMISTRY, 286(34), 30219-30231, 20110826
  21. Structural evidence that puromycin hydrolase is a new type of aminopeptidase with a prolyl oligopeptidase family fold, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 79(10), 2999-3005, 201110
  22. Catalytic Mechanism of Bleomycin N-Acetyltransferase Proposed on the Basis of Its Crystal Structure, JOURNAL OF BIOLOGICAL CHEMISTRY, 285(2), 1446-1456, 20100108
  23. Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(2), 289-293, 201002
  24. ★, Molecular Cloning and Heterologous Expression of a Biosynthetic Gene Cluster for the Antitubercular Agent D-Cycloserine Produced by Streptomyces lavendulae, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 54(3), 1132-1139, 201003
  25. Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(10), 1673-1679, 201010
  26. Crystal structure and mutagenic analysis of a bacteriocin immunity protein, Mun-im, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 378(3), 574-578, 20090116
  27. Crystal structure of SCCA1 and insight about the interaction with JNK1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 380(1), 143-147, 20090227
  28. Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli, MICROBIOLOGY-SGM, 155, 1726-1737, 200905
  29. A novel tyrosine-phosphorylated protein inhibiting the growth of Streptomyces cells, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 385(4), 534-538, 20090807
  30. Molecular Cloning and Functional Analysis of minD Gene from Streptomyces lavendulae ATCC25233, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(3), 303-305, 200809
  31. Self-protection mechanisms of antibiotic-producing microorganisms, Nippon rinsyo, 65, 669-673, 20070228
  32. ★, Cloning and characterization of a Streptomyces single module type non-ribosomal peptide synthetase catalyzing a blue pigment synthesis, JOURNAL OF BIOLOGICAL CHEMISTRY, 282(12), 9073-9081, 20070323
  33. Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis, JOURNAL OF BIOLOGICAL CHEMISTRY, 281(13), 8981-8990, 20060331
  34. Purification and characterization of the second Streptomyces phospholipase A(2) refolded from an inclusion body, PROTEIN EXPRESSION AND PURIFICATION, 50(1), 82-88, 200611
  35. The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: Crystallographic analysis to elucidate the binding mode of the antibiotic to the protein, JOURNAL OF MOLECULAR BIOLOGY, 360(2), 398-408, 20060707
  36. Identification and kinetic study of tyrosinase inhibitors found in sake lees, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 54(26), 9827-9833, 20061227
  37. A novel assay method for an amino acid racemase reaction based on circular dichroism, BIOCHEMICAL JOURNAL, 389, 491-496, 20050715
  38. An efficient method for the overexpression and purification of active tyrosinase from Streptomyces castaneoglobisporus, PROTEIN EXPRESSION AND PURIFICATION, 34(2), 202-207, 200404
  39. Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae - Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(44), 46143-46152, 20041029
  40. Structural evidence that alanine racemase from a D-cycloserine-producing microorganism exhibits resistance to its own product, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(44), 46153-46161, 20041029
  41. Cell differentiation and p38(MAPK) cascade are inhibited in human osteoblasts cultured in a three-dimensional clinostat., In Vitro Cell Dev Biol Anim, 39(1-2), 89-97, 20030101
  42. Physical stress by magnetic force accelerates differentiation of human osteoblasts, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 311(1), 32-38, 20031107
  43. Molecular mechanism for the enhancement of arbekacin resistance in a methicillin-resistant Staphylococcus aureus, FEBS LETTERS, 546(2-3), 401-406, 20030710
  44. Molecular cloning of a D-cycloserine resistance gene from D-cycloserine-producing Streptomyces garyphalus, JOURNAL OF ANTIBIOTICS, 56(9), 762-767, 200309
  45. Streptomyces Phospholipase A2: Its Use in the Enzymatic Measurement of Calcium(II) Ion Content, Actinomycetologica, 16(2), 17-20, 20020401
  46. Molecular mechanisms for bleomycin resistance, Tanpakushitsu Kakusan Koso, 47(10), 1276-1284, 20020401
  47. The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus, JOURNAL OF BIOLOGICAL CHEMISTRY, 277(3), 2311-2320, 20020118
  48. Molecular and structural biology of bleomycin and its resistance determinants, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93(2), 105-116, 200202
  49. Use of bleomycin- and heat shock-induced calreticulin promoter for construction of a mammalian expression vector, JOURNAL OF BIOCHEMISTRY, 129(5), 671-674, 200105
  50. Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin, JOURNAL OF BIOLOGICAL CHEMISTRY, 276(13), 9992-9999, 20010330
  51. The 1.5 angstrom crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus, JOURNAL OF MOLECULAR BIOLOGY, 295(4), 915-925, 20000128
  52. Crystallization and preliminary X-ray diffraction studies of Streptomyces phospholipase A(2) in a calcium-binding form, JOURNAL OF INORGANIC BIOCHEMISTRY, 82(1-4), 221-223, 200011
  53. Characterization of the bleomycin resistance determinant encoded on the transposon Tn5, FEBS Lett, 442, 34-38, 19990401
  54. Mutation of the N-terminal Proline 9 of BLMA from Streptomyces verticillus abolishes the binding affinity for bleomycin, FEBS Lett, 450, 227-230, 19990401
  55. Crystallization and preliminary X-ray diffraction studies of bleomycin-binding protein encoded on the transposon Tn5, Acta Crystallogr. Sect. D, 55, 1095-1097, 19990501
  56. A mammalian vector carrying the bleomycin N-acetyltransferase gene from Streptomyces verticillus as a selective marker, J. Antibiot., 52, 895-899, 19990401
  57. Protection of mammalian cells from the toxicity of bleomycin by expression of a bleomycin-binding protein gene from Streptomyces verticillus, J. Biochem., 124, 835-841, 19980401
  58. Crystallization and preliminary X-ray diffraction studies of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus, Acta Crystallogr. Sect. D, 54, 127-128, 19980101
  59. Development of a useful vector system that enables visual detection of inhibitor of nucleic acid synthesis, J. Ferment. Bioeng., 84, 155-157, 19970401
  60. Blepharismin produced by a protozoan Blepharisma functions as an antibiotic effective against methicillin-resistant Staphylococcus aureus, FEMS Microbiol. Lett., 155, 67-71, 19970401
  61. Growth of human pancreatic cancer cells, induced by human pancreatic phospholipase A2, is mediated via its specific receptor but not via its catalytic property, INTERNATIONAL JOURNAL OF ONCOLOGY, 9, 1219-1225, 19961201
  62. Overproduction of the bleomycin-binding proteins from bleomycin-producing Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus in Escherichia coli and their immunological characterisation, FEBS Lett, 362, 80-84, 19950401
  63. Gene technology to overproduce the enzymes useful as diagnostic reagents, Rinsho Byori, 43(8), 765-771, 19950801
  64. Inactivation of bleomycin by an N-acetyltransferase in the bleomycin-producing strain Streptomyces verticillus, FEMS Microbiology Letters, 121, 81-86, 19940401
  65. Characterisation by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin, Gene, 151, 11-16, 19940401