Takanori Kumagai

Last Updated :2024/06/04

Affiliations, Positions
Graduate School of Biomedical and Health Sciences(Pharmaceutical Sciences), Associate Professor
E-mail
tkumahiroshima-u.ac.jp
Self-introduction
I carry out studies using some kind of microorganism, called 'Actinomycetes', known as producers of various beneficial secondary metabolites.

Basic Information

Major Professional Backgrounds

  • 1995/04/01, 2003/03/31, Department of Pharmaceutical Sciences, Hiroshima University, Research Associate
  • 2003/04/01, 2004/01/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Assistant Professor
  • 2004/02/01, 2007/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Associate professor
  • 2007/04/01, 2012/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical Sciences, Hiroshima University, Associate professor
  • 2012/04/01, 2019/03/31, Department of Molecular Microbiology and Biotechnology, Graduate School of Biomedical and Health Sciences, Hiroshima University, Associate professor
  • 2019/04/01, Graduate School of Biomedical and Health Sciences, Associate Professor

Academic Degrees

  • Master of Pharmaceutical Science, Hiroshima University
  • Doctor of Philosophy in Pharmaceutical Science, Hiroshima University

Educational Activity

  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Pharmaceutical Sciences : Pharmaceutical Sciences
  • [Bachelor Degree Program] School of Pharmaceutical Sciences : Program of Medicinal Sciences : Medicinal Sciences
  • [Master's Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences
  • [Doctoral Program] Graduate School of Biomedical and Health Sciences : Division of Integrated Health Sciences : Program of Medicinal Sciences

Research Fields

  • Medicine,dentistry, and pharmacy;Pharmacy;Natural medicines

Research Keywords

  • secondary metabolism
  • Genome analysis
  • heterologous production
  • antibiotic-producer
  • antibiotics

Affiliated Academic Societies

  • The Society for Actinomycetes Japan, 1994/04
  • The Society for Biotechnology Japan, 1995/04
  • The Japanese Biochemical Society, 2005/04
  • The Pharmaceutical Society of Japan, 2005/04
  • Japanese Society for Bacteriology, 2018/02

Educational Activity

Course in Charge

  1. 2024, Undergraduate Education, 4Term, Biochemistry II
  2. 2024, Undergraduate Education, First Semester, Practice of Microbiology
  3. 2024, Undergraduate Education, 3Term, Genetic Engineering
  4. 2024, Undergraduate Education, Intensive, Genetic Engineering
  5. 2024, Undergraduate Education, First Semester, Experiments of Microbial Chemistry
  6. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences I
  7. 2024, Undergraduate Education, Year, Special laboratory Works in Pharmaceutical Sciences II
  8. 2024, Undergraduate Education, Second Semester, Special laboratory Works in Pharmaceutical Sciences III
  9. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy I
  10. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy II
  11. 2024, Undergraduate Education, Year, Special laboratory Works in Clinical Pharmacy III
  12. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Microbiology
  13. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Microbiology
  14. 2024, Graduate Education (Master's Program) , First Semester, Research on Microbiology
  15. 2024, Graduate Education (Master's Program) , Second Semester, Research on Microbiology
  16. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Microbiology
  17. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Microbiology
  18. 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Microbiology
  19. 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Microbiology
  20. 2024, Graduate Education (Master's Program) , First Semester, Seminar on Antibiotic Biosynthesis
  21. 2024, Graduate Education (Master's Program) , Second Semester, Seminar on Antibiotic Biosynthesis

Research Activities

Academic Papers

  1. Construction of a temperature-sensitive shuttle vector between lactic acid bacteria and Escherichia coli and its application to a Tn10-based random mutagenesis tool in lactic acid bacteria, Biol. Pharm. Bull., 46, 840-847, 20230601
  2. Effects of the order of exposure to antimicrobials on the incidence of multidrug-resistant Pseudomonas aeruginosa, Sci. Rep., 13, 8826, 20230531
  3. Whole-genome and plasmid comparative analysis of Campylobacter jejuni from human patients Toyama, Japan, from 2015 to 2019, Microbiol. Spectr., 11(1), e02659-22, 20230109
  4. Genome sequence-guided finding of lucensomycin production by Streptomyces achromogenes subsp. streptozoticus NBRC14001, Microorganisms, 10(1), 37, 20211226
    Link to Paper Search Results by DOI
  5. Catalytic specificity of the Lactobacillus plantarum cystathionine gamma-lyase presumed by the crystallographic analysis, SCIENTIFIC REPORTS, 10(1), 14886, 20200910
    Link to Paper Search Results by DOI
  6. Comprehensive analysis of resistance-nodulation-cell division superfamily (RND) efflux pumps from Serratia marcescens, Db10, Scientific Reports, 9(1), 4854, 201903
  7. Expression of Genes Involved in Bacteriocin Production and Self-Resistance in Lactobacillus brevis 174A Is Mediated by Two Regulatory Proteins, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 84(7), e02707-e02717, 201804
    Link to Paper Search Results by DOI
  8. Characterization of the SN35N Strain-Specific Exopolysaccharide Encoded in the Whole Circular Genome of a Plant-Derived Lactobacillus plantarum, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 41(4), 536-545, 201804
  9. Characterization of Exopolysaccharides Produced by Thermophilic Lactic Acid Bacteria Isolated from Tropical Fruits of Thailand, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 40(5), 621-629, 201705
  10. Antiobesity effect of Pediococcus pentosaceus LP28 on overweight subjects: a randomized, double-blind, placebo-controlled clinical trial, EUROPEAN JOURNAL OF CLINICAL NUTRITION, 70(5), 582-587, 201605
    Link to Paper Search Results by DOI
  11. Characterization of the LP28 starin-specific exopolysaccharide biosynthetic gene cluster found in the whole circular genome of Pediococcus pentosaceus, Biochemistry and Biophysics Reports, 5, 266-271, 2016
  12. Oral lactic acid bacteria that are related to occurrence and/or progression of dental caries in Japanese preschool children, Biosci. Microbiota Food Health, 34, 29-36, 2015
  13. The structural and mutational analyses of O-ureido-l-serine synthase necessary for d-cycloserine biosynthesis, FEBS JOURNAL, 282(20), 3929-3944, 201510
    Link to Paper Search Results by DOI
  14. ★, High-Level Heterologous Production of D-Cycloserine by Escherichia coli, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 81(22), 7881-7887, 201511
    Link to Paper Search Results by DOI
  15. Characterization and Mutational Analysis of a Two-Polypeptide Bacteriocin Produced by Citrus Iyo-Derived Lactobacillus brevis 174A, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 38(12), 1902-1909, 201512
  16. An alternative allosteric regulation mechanism of an acidophilic L-lactate dehydrogenase from Enterococcus mundtii 15-1A, FEBS OPEN BIO, 4, 834-847, 2014
    Link to Paper Search Results by DOI
  17. Aspergillus oryzae S-03 produces gingipain inhibitors as a virulence factor for Porphyromonas gingivalis, J. Bacteriol. Virol., 44, 152-161, 2014
  18. Establishment of an In Vitro D-Cycloserine-Synthesizing System by Using O-Ureido-L-Serine Synthase and D-Cycloserine Synthetase Found in the Biosynthetic Pathway, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 57(6), 2603-2612, 201306
    Link to Paper Search Results by DOI
  19. Crystallographic Study To Determine the Substrate Specificity of an L-Serine-Acetylating Enzyme Found in the D-Cycloserine Biosynthetic Pathway, JOURNAL OF BACTERIOLOGY, 195(8), 1741-1749, 201304
    Link to Paper Search Results by DOI
  20. Crystallographic and mutational analyses of tannase from Lactobacillus plantarum, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 81(11), 2052-2058, 201311
    Link to Paper Search Results by DOI
  21. The Obesity and Fatty Liver Are Reduced by Plant-Derived Pediococcus pentosaceus LP28 in High Fat Diet-Induced Obese Mice, PLOS ONE, 7(2), 20120217
    Link to Paper Search Results by DOI
  22. ★, Heme Protein and Hydroxyarginase Necessary for Biosynthesis of D-Cycloserine, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 56(7), 3682-3689, 201207
    Link to Paper Search Results by DOI
  23. A Molecular Mechanism for Copper Transportation to Tyrosinase That Is Assisted by a Metallochaperone, Caddie Protein, JOURNAL OF BIOLOGICAL CHEMISTRY, 286(34), 30219-30231, 20110826
    Link to Paper Search Results by DOI
  24. Structural evidence that puromycin hydrolase is a new type of aminopeptidase with a prolyl oligopeptidase family fold, PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS, 79(10), 2999-3005, 201110
    Link to Paper Search Results by DOI
  25. Catalytic Mechanism of Bleomycin N-Acetyltransferase Proposed on the Basis of Its Crystal Structure, JOURNAL OF BIOLOGICAL CHEMISTRY, 285(2), 1446-1456, 20100108
    Link to Paper Search Results by DOI
  26. Establishment of an in Vitro Peyer's Patch Cell Culture System Correlative to in Vivo Study Using Intestine and Screening of Lactic Acid Bacteria Enhancing Intestinal Immunity, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(2), 289-293, 201002
    Link to Paper Search Results by DOI
  27. ★, Molecular Cloning and Heterologous Expression of a Biosynthetic Gene Cluster for the Antitubercular Agent D-Cycloserine Produced by Streptomyces lavendulae, ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 54(3), 1132-1139, 201003
    Link to Paper Search Results by DOI
  28. Establishment of an Efficient Fermentation System of Gamma-Aminobutyric Acid by a Lactic Acid Bacterium, Enterococcus avium G-15, Isolated from Carrot Leaves, BIOLOGICAL & PHARMACEUTICAL BULLETIN, 33(10), 1673-1679, 201010
    Link to Paper Search Results by DOI
  29. Crystal structure and mutagenic analysis of a bacteriocin immunity protein, Mun-im, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 378(3), 574-578, 20090116
    Link to Paper Search Results by DOI
  30. Crystal structure of SCCA1 and insight about the interaction with JNK1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 380(1), 143-147, 20090227
    Link to Paper Search Results by DOI
  31. Characterization of four plasmids harboured in a Lactobacillus brevis strain encoding a novel bacteriocin, brevicin 925A, and construction of a shuttle vector for lactic acid bacteria and Escherichia coli, MICROBIOLOGY-SGM, 155, 1726-1737, 200905
    Link to Paper Search Results by DOI
  32. A novel tyrosine-phosphorylated protein inhibiting the growth of Streptomyces cells, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 385(4), 534-538, 20090807
    Link to Paper Search Results by DOI
  33. Molecular Cloning and Functional Analysis of minD Gene from Streptomyces lavendulae ATCC25233, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 106(3), 303-305, 200809
    Link to Paper Search Results by DOI
  34. Self-protection mechanisms of antibiotic-producing microorganisms, Nippon rinsyo, 65, 669-673, 20070228
  35. ★, Cloning and characterization of a Streptomyces single module type non-ribosomal peptide synthetase catalyzing a blue pigment synthesis, JOURNAL OF BIOLOGICAL CHEMISTRY, 282(12), 9073-9081, 20070323
    Link to Paper Search Results by DOI
  36. Crystallographic evidence that the dinuclear copper center of tyrosinase is flexible during catalysis, JOURNAL OF BIOLOGICAL CHEMISTRY, 281(13), 8981-8990, 20060331
    Link to Paper Search Results by DOI
  37. Purification and characterization of the second Streptomyces phospholipase A(2) refolded from an inclusion body, PROTEIN EXPRESSION AND PURIFICATION, 50(1), 82-88, 200611
    Link to Paper Search Results by DOI
  38. The mitomycin C (MMC)-binding protein from MMC-producing microorganisms protects from the lethal effect of bleomycin: Crystallographic analysis to elucidate the binding mode of the antibiotic to the protein, JOURNAL OF MOLECULAR BIOLOGY, 360(2), 398-408, 20060707
    Link to Paper Search Results by DOI
  39. Identification and kinetic study of tyrosinase inhibitors found in sake lees, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 54(26), 9827-9833, 20061227
    Link to Paper Search Results by DOI
  40. A novel assay method for an amino acid racemase reaction based on circular dichroism, BIOCHEMICAL JOURNAL, 389, 491-496, 20050715
    Link to Paper Search Results by DOI
  41. An efficient method for the overexpression and purification of active tyrosinase from Streptomyces castaneoglobisporus, PROTEIN EXPRESSION AND PURIFICATION, 34(2), 202-207, 200404
    Link to Paper Search Results by DOI
  42. Self-protection mechanism in D-cycloserine-producing Streptomyces lavendulae - Gene cloning, characterization, and kinetics of its alanine racemase and D-alanyl-D-alanine ligase, which are target enzymes of D-cycloserine, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(44), 46143-46152, 20041029
    Link to Paper Search Results by DOI
  43. Structural evidence that alanine racemase from a D-cycloserine-producing microorganism exhibits resistance to its own product, JOURNAL OF BIOLOGICAL CHEMISTRY, 279(44), 46153-46161, 20041029
    Link to Paper Search Results by DOI
  44. Cell differentiation and p38(MAPK) cascade are inhibited in human osteoblasts cultured in a three-dimensional clinostat., In Vitro Cell Dev Biol Anim, 39(1-2), 89-97, 20030101
  45. Physical stress by magnetic force accelerates differentiation of human osteoblasts, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 311(1), 32-38, 20031107
    Link to Paper Search Results by DOI
  46. Molecular mechanism for the enhancement of arbekacin resistance in a methicillin-resistant Staphylococcus aureus, FEBS LETTERS, 546(2-3), 401-406, 20030710
    Link to Paper Search Results by DOI
  47. Molecular cloning of a D-cycloserine resistance gene from D-cycloserine-producing Streptomyces garyphalus, JOURNAL OF ANTIBIOTICS, 56(9), 762-767, 200309
    Link to Paper Search Results by DOI
  48. Streptomyces Phospholipase A2: Its Use in the Enzymatic Measurement of Calcium(II) Ion Content, Actinomycetologica, 16(2), 17-20, 20020401
  49. Molecular mechanisms for bleomycin resistance, Tanpakushitsu Kakusan Koso, 47(10), 1276-1284, 20020401
  50. The 1.6-A crystal structure of the copper(II)-bound bleomycin complexed with the bleomycin-binding protein from bleomycin-producing Streptomyces verticillus, JOURNAL OF BIOLOGICAL CHEMISTRY, 277(3), 2311-2320, 20020118
    Link to Paper Search Results by DOI
  51. Molecular and structural biology of bleomycin and its resistance determinants, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 93(2), 105-116, 200202
    Link to Paper Search Results by DOI
  52. Use of bleomycin- and heat shock-induced calreticulin promoter for construction of a mammalian expression vector, JOURNAL OF BIOCHEMISTRY, 129(5), 671-674, 200105
    Link to Paper Search Results by DOI
  53. Crystal structures of the transposon Tn5-carried bleomycin resistance determinant uncomplexed and complexed with bleomycin, JOURNAL OF BIOLOGICAL CHEMISTRY, 276(13), 9992-9999, 20010330
    Link to Paper Search Results by DOI
  54. The 1.5 angstrom crystal structure of a bleomycin resistance determinant from bleomycin-producing Streptomyces verticillus, JOURNAL OF MOLECULAR BIOLOGY, 295(4), 915-925, 20000128
    Link to Paper Search Results by DOI
  55. Crystallization and preliminary X-ray diffraction studies of Streptomyces phospholipase A(2) in a calcium-binding form, JOURNAL OF INORGANIC BIOCHEMISTRY, 82(1-4), 221-223, 200011
    Link to Paper Search Results by DOI
  56. Characterization of the bleomycin resistance determinant encoded on the transposon Tn5, FEBS Lett, 442, 34-38, 19990401
  57. Mutation of the N-terminal Proline 9 of BLMA from Streptomyces verticillus abolishes the binding affinity for bleomycin, FEBS Lett, 450, 227-230, 19990401
  58. Crystallization and preliminary X-ray diffraction studies of bleomycin-binding protein encoded on the transposon Tn5, Acta Crystallogr. Sect. D, 55, 1095-1097, 19990501
  59. A mammalian vector carrying the bleomycin N-acetyltransferase gene from Streptomyces verticillus as a selective marker, J. Antibiot., 52, 895-899, 19990401
  60. Protection of mammalian cells from the toxicity of bleomycin by expression of a bleomycin-binding protein gene from Streptomyces verticillus, J. Biochem., 124, 835-841, 19980401
  61. Crystallization and preliminary X-ray diffraction studies of bleomycin-binding protein from bleomycin-producing Streptomyces verticillus, Acta Crystallogr. Sect. D, 54, 127-128, 19980101
  62. Development of a useful vector system that enables visual detection of inhibitor of nucleic acid synthesis, J. Ferment. Bioeng., 84, 155-157, 19970401
  63. Blepharismin produced by a protozoan Blepharisma functions as an antibiotic effective against methicillin-resistant Staphylococcus aureus, FEMS Microbiol. Lett., 155, 67-71, 19970401
  64. Growth of human pancreatic cancer cells, induced by human pancreatic phospholipase A2, is mediated via its specific receptor but not via its catalytic property, INTERNATIONAL JOURNAL OF ONCOLOGY, 9, 1219-1225, 19961201
  65. Overproduction of the bleomycin-binding proteins from bleomycin-producing Streptomyces verticillus and a methicillin-resistant Staphylococcus aureus in Escherichia coli and their immunological characterisation, FEBS Lett, 362, 80-84, 19950401
  66. Gene technology to overproduce the enzymes useful as diagnostic reagents, Rinsho Byori, 43(8), 765-771, 19950801
  67. Inactivation of bleomycin by an N-acetyltransferase in the bleomycin-producing strain Streptomyces verticillus, FEMS Microbiology Letters, 121, 81-86, 19940401
  68. Characterisation by molecular cloning of two genes from Streptomyces verticillus encoding resistance to bleomycin, Gene, 151, 11-16, 19940401