稲生 雄大YUDAI INABU

Last Updated :2026/02/10

所属・職名
大学院統合生命科学研究科　助教
メールアドレス
inabuhiroshima-u.ac.jp
その他連絡先
東広島市鏡山1-4-4 生物生産学部B棟B517室
TEL:0824247957

基本情報

主な職歴

  • 2019年04月, 2019年09月, 日本学術振興会, 特別研究員（DC2)
  • 2019年10月, 2023年09月, 九州大学, 助教
  • 2023年10月01日, 広島大学, 大学院統合生命科学研究科, 助教

学歴

  • 広島大学, 大学院生物圏科学研究科, 博士課程後期生物資源科学専攻, 日本, 2017年04月, 2019年09月
  • 広島大学, 大学院生物圏科学研究科, 博士課程前期生物資源科学専攻, 日本, 2015年04月, 2017年03月
  • 広島大学, 生物生産学部, 生物生産学科, 日本, 2011年04月, 2015年03月

学位

  • 博士（農学）　(広島大学)
  • 修士（農学）　(広島大学)

研究分野

  • 農学 / 動物生命科学 / 動物生産科学

研究キーワード

  • グルカゴン様ペプチド
  • 内分泌
  • 肉牛
  • 乳牛

所属学会

  • 日本暖地畜産学会
  • アメリカ酪農科学学会(ADSA)
  • 日本畜産学会

教育活動

授業担当

  1. 2025年, 学部専門, 集中, 基礎物理学実験
  2. 2025年, 学部専門, ３ターム, 生物資源科学リサーチフロント
  3. 2025年, 学部専門, ４ターム, 動物環境生理学
  4. 2025年, 学部専門, ２ターム, 応用動植物科学外書講読
  5. 2025年, 学部専門, ２ターム, 動物生産管理学
  6. 2025年, 学部専門, 集中, 動物生産学実験II
  7. 2025年, 学部専門, セメスター（後期）, 卒業論文I
  8. 2025年, 学部専門, セメスター（前期）, 卒業論文II
  9. 2025年, 学部専門, セメスター（後期）, 卒業論文III
  10. 2025年, 学部専門, 集中, (AIMS) Introduction Physiology of Domestic Animals
  11. 2025年, 学部専門, 集中, Introductory Physiology of Domestic Animals
  12. 2025年, 博士課程・博士課程後期, １ターム, 生命科学研究計画法
  13. 2025年, 修士課程・博士課程前期, ３ターム, 応用動物生産論

研究活動

学術論文（★は代表的な論文）

  1. Maternal administration of octanoate, a medium-chain fatty acid, improves feed efficiency of Japanese black calves through influencing gut bacteriome structure, Scientific Reports, 15巻, 33557号, 20250929
    DOIを用いた論文検索結果へのリンク
  2. Effect of Nitrate Supplementation on Digestion, Rumen Fermentation, Plasma Metabolites, and Nitrogen Use in Sheep Fed a High-Forage Diet, ANIMAL SCIENCE JOURNAL, 97巻, 1号, 20260106
    DOIを用いた論文検索結果へのリンク
  3. Comparison of milk production and endocrine profiles of dairy cows exposed to either white light-emitting diode or induction lighting, Domestic Animal Endocrinology, 93巻, 106958号, 20250613
    DOIを用いた論文検索結果へのリンク
  4. ★, Effect of feeding sodium butyrate to beef female cows during pre- and post-partum period on concentrations of glucagon-like peptides in plasma and colostrum, ANIMAL SCIENCE JOURNAL, 95巻, 1号, 202401
    DOIを用いた論文検索結果へのリンク
  5. Compost fermented with thermophilic Bacillaceae reduces heat stress–induced mortality in laying hens through gut microbial modulation, Animal Microbiome, 8巻, 9号, 20260203
    DOIを用いた論文検索結果へのリンク
  6. 乳用仔牛におけるグルカゴン様ペプチドの分泌と生理的な作用に関する研究, 家畜栄養生理研究会報, 64巻, 1-2号, pp. 33-41, 2020
  7. Effect of Ca-octanoate supplementation on concentrations of ghrelin and ghrelin-related hormones in plasma and milk of beef cattle, Heliyon, 9巻, 6号, pp. e16795, 202306
  8. Estimation of silent phenotypes of calf antibiotic dysbiosis, Scientific Reports, 13巻, pp. 6359, 202304
  9. Effect of kraft pulp inclusion in calf starter on performance, health, and plasma concentration of glucagon-like peptide 2 in calves, Journal of Dairy Science, 106巻, 6号, pp. 4443-4453, 202306
  10. Development of a novel feeding method for Japanese black calves with thermophile probiotics at postweaning, Journal of Applied Microbiology, 132巻, 5号, pp. 3870-3882, 202203
  11. Rumen microbiota and its relation to fermentation in lactose-fed calves, Journal of Dairy Science, 104巻, 10号, pp. 10744-10752, 202110
  12. Glucagon-like peptide 2 (GLP-2) in bovine colostrum and transition milk, Heliyon, 7巻, 5号, pp. e07046, 202105
  13. Effects of starch concentration of close-up diets on rumen pH and plasma metabolite responses of dairy cows to grain challenges after calving, Journal of Dairy Science, 103巻, 12号, pp. 11461-11471, 202012
  14. Effects of feeding high volumes of milk replacer on reproductive performance and on concentrations of metabolites and hormones in blood of Japanese black heifer calves, Animal Science Journal, 92巻, 1号, pp. e13505, 202101
  15. Effects of pre- and postpartum dietary starch content on productivity, plasma energy metabolites, and serum inflammation indicators of dairy cows, Journal of Dairy Science, 104巻, 4号, pp. 4362-4374, 202104
  16. ★, Effects of feeding a moderate- or high-energy close-up diet to cows on response of newborn calves to milk replacer feeding and intravenous injection of glucagon-like peptide 1, Domestic Animal Endocrinology, 74巻, 106528号, 202101
  17. Feeding colostrum or a 1:1 colostrum:milk mixture for 3 days postnatal increases small intestinal development and minimally influences plasma glucagon-like peptide-2 and serum insulin-like growth factor-1 concentrations in Holstein bull calves, Journal of Dairy Science, 103巻, 5号, pp. 4236-4251, 202003
  18. Effects of feeding a high- or moderate-starch prepartum diet to cows on newborn dairy heifer calf responses to intravenous glucose tolerance tests early in life, Journal of Dairy Science, 102巻, 10号, pp. 8931-8940, 201910
  19. The effect of tributyrin supplementation to milk replacer on plasma glucagon-like peptide 2 concentrations in pre-weaning calves, Animal Science Journal, 90巻, 9号, pp. 1185-1192, 201909
  20. ★, Effect of extended colostrum feeding on plasma glucagon-like peptide-1 concentration in newborn calves, Journal of Dairy Science, 102巻, 5号, pp. 4619-4627, 201903
  21. Effects of pulse-dose ruminal infusion of butyrate on plasma glucagon-like peptide 1 and 2 concentrations in dairy calves, Journal of Dairy Science, 102巻, 3号, pp. 2254-2265, 201903
  22. ★, Short communication: The effect of delayed colostrum feeding on plasma concentrations of glucagon-like peptide 1 and 2 in newborn calves, Journal of Dairy Science, 101巻, 7号, pp. 6627-6631, 201807
  23. ★, Plasma concentrations of glucagon-like peptide 1 and 2 in calves fed calf starters containing lactose, Journal of Dairy Science, 100巻, 11号, pp. 9361-9371, 201709
  24. Response of plasma glucagon-like peptide-2 to feeding pattern and intraruminal administration of volatile fatty acids in sheep, Domestic Animal Endocrinology, 60巻, pp. 31-41, 201707
  25. Effects of partial replacement of corn grain with lactose in calf starters on ruminal fermentation and growth performance, Journal of Dairy Science, 100巻, 8号, pp. 6177-6186, 201708

招待講演、口頭・ポスター発表等

  1. 乳用子牛への酪酸製剤給与がグルカゴン様ペプチド1分泌を介した糖代謝に及ぼす影響, 稲生　雄大・續宗　汰朗・杉野　利久・小櫃　剛人, 日本畜産学会第133回大会, 2025年09月, 通常, 日本語, 【目的】グルカゴン様ペプチド1(GLP-1)はインスリンの分泌促進や作用増強に関与し、その分泌は酪酸により刺激される。本研究は、代用乳への酪酸製剤添加によるGLP-1分泌促進が子牛の糖代謝に及ぼす影響を検討した。【方法】4週齢のホルスタイン種雌子牛10頭を供試し、代用乳を１日２回等分給与し、８週齢で離乳させた。代用乳に酪酸製剤を1日10g添加する酪酸区、添加しない対照区の２区を設けた。採血は毎週朝の哺乳前に実施した。さらに、4および8週齢時に耐糖能試験（GTT）を、6週齢時の代用乳給与前後で連続採血を行う食餌負荷試験（MTT）を実施し、血漿代謝物・ホルモン濃度を測定した。【結果】MTTにおいて、血漿GLP-1濃度は酪酸区で高値を示したが、インスリンを含むその他血漿成分に処理区間の差はなかった。5週齢時のGTTにおいて、血漿グルコース濃度は酪酸区で高値を示す傾向にあり、NEFA濃度はグルコース注入10分後において酪酸区で低値を示した。一方、8週齢時のGTTでは、血漿成分に処理区間の差はなかった。哺乳前において、血漿ASTおよびALT濃度は酪酸区において低値を示した。以上のことから、代用乳への酪酸製剤添加は、子牛の血漿GLP-1濃度を増加させるとともに、脂質代謝や肝機能に影響する可能性が示されたが、糖代謝に及ぼす影響は明確ではなかった。
  2. 分娩移行期における黒毛和種繁殖雌牛への酪酸ナトリウム給与がグルカゴン様ペプチドの血漿および乳中濃度に及ぼす影響, 稲生雄大・堀家洋志・山野晴樹・田口佑充・岡田隼之介・衛藤哲次・塩塚雄二・藤野亮一・髙橋秀之, 日本畜産学会第132回大会, 2024年09月, 通常, 日本語, 【目的】腸管L細胞から分泌される消化管ホルモンであるグルカゴン様ペプチド（GLP）-1および2の分泌は、食飼性酪酸によって強力に刺激される。初乳に含まれるホルモンは子牛の発育や健全性に重要な役割を担っており、これは母牛の血中から移行する。本研究は、分娩移行期の肉用牛への酪酸ナトリウム（SB）給与が、GLP-1・GLP-2の血漿、初乳および移行乳中濃度に及ぼす影響を検討した。【方法】黒毛和種繁殖雌牛12頭を供試し、分娩予定日60日前から分娩後3日まで、乾草および配合飼料を制限給与した。対照区および酪酸区の2区を設け、酪酸区では、給飼量の乾物あたり1.1％のSBを添加給与した。採血は分娩予定日の60、30、7日前、並びに分娩後0～3日に、乳サンプル採取は分娩後の採血後に実施し、代謝物およびホルモン濃度を測定した。【結果】血漿GLP-1濃度は分娩後3日において、酪酸区が対照区と比較し高値であった。一方、血漿GLP-2濃度は処理による影響を受けなかった。本研究により、GLP-1 およびGLP-2 がウシ初乳中に血漿濃度と比較し高濃度で含まれることが明らかとなった。一方、乳中代謝物およびホルモン濃度に処理区間の差は認められなかった。以上のことから、分娩移行期の肉用牛へのSB給与は分娩後の血漿GLP-1濃度を増加させたが、初乳・移行乳成分には明確に影響しないことが示唆された。
  3. Effect of feeding sodium butyrate to beef female cows during calving transition period on glucagon-like peptide 1 and 2 concentrations in colostrum and transition milk., 稲生雄大・堀家洋志・山野晴樹・田口佑充・岡田隼之介・衛藤哲次・塩塚雄二・藤野亮一・髙橋秀之, International Symposium on Ruminant Physiology (ISRP), 2024年, 通常, 英語, シカゴ, Secretion of glucagon-like peptide (GLP)-1 and 2 (GLP-2), a gut-derived peptide secreted from intestinal L-cells, is potently stimulated by dietary butyrate provision. Since the hormones contained in milk are transferred from the maternal blood circulation, an increase in circulating levels of GLP-1 and 2 due to dietary butyrate could result in increased levels in colostrum and transition milk. This study evaluated the effect of feeding beef cows with sodium butyrate (SB) during the late pregnancy and early post-partum periods on concentrations of GLP-1 and 2 in plasma, colostrum, and transition milk. Twelve Japanese Black female cows were fed hay and concentrate according to Japanese feeding standard for beef cows (2008) with (BUTY) or without (CON; n = 6 for each treatment) SB supplementation at 1.5% of the dietary dry matter from 60 d before the expected calving date (−60 d) to 4 d after calving. Blood samples were collected 1 h before feeding on −60, −30, and −7 d, and 0 (calving date), 1, 2, and 3 d after calving. Milk samples were collected immediately after blood sampling post-partum. Data were analyzed by ANOVA of JMP® 14 using fit model procedure. Plasma total cholesterol concentration was higher (P = 0.04) for the BUTY (151 ± 6.27 mg/dL) than for the CON (131 ± 6.27 mg/dL). In addition, plasma GLP-1 concentration was higher for the BUTY (0.82 ± 0.092 ng/mL) than for the CON (0.50 ± 0.092) at 3 d after calving (P < 0.05). On the other hand, plasma GLP-2 concentration was not affected by treatment. This study showed for the first time that GLP-1 and 2 were present in bovine colostrum at higher concentrations as compared to in plasma at −7 and 0 d (P < 0.01). On the other hand, treatment did not affect metabolite and hormone concentrations in colostrum and transition milk. In summary, feeding beef cows with SB during the calving transition period likely increases plasma GLP-1 concentrations post-partum without affecting the components of colostrum and transition milk.
  4. Effects of exposure to white LED or electrodeless induction lamp on milk production performance and blood compositions of dairy cows, Inabu Y, Takakura Y, Shinohara Y, Sunadome M, Watanabe R, Kushibiki S, Obitsu T, Sugino T., ADSA Annual Meeting, 2024年, 通常, 英語, Electrodeless induction lamp (EIL) provides lower blue light as compared to white LED (WLED) and are now widely used in various facilities. The objective of this study was to evaluate the response of milk production and physiological parameters to exposure to WLED or EIL in Holstein cows. Ten Holstein lactating cows (225 ± 32.5 DIM, 710 ± 24.6 kg initial BW, 2.56 ± 1.59 parity) were managed under the long-day photoperiod (16:8 h light-dark cycle) and were assigned to two treatments for 3 weeks each in a 2 by 2 crossover design as follows: exposure to WLED (453 nm peak wavelength, 231 Lux) or EIL (550nm peak wavelength, 237 Lux) during daytime. The dark period (21:00 h to 5:00 h) for all treatments was no illumination (0.0 lux). All cows were fed same TMR ad libitum throughout the experiment. Milk samples were collected every week to measure milk compositions. Serial blood sampling was performed on the last day of each treatment to measure metabolite and hormone concentrations in plasma. Data were analyzed by ANOVA using Fit Model procedure of JMP® 17 pro (SAS Institute Inc., Cary, NC). Dry matter intake, BW, milk yield and composition, rumination time and plasma metabolite concentrations were not affected by light treatment. Contrary to our hypothesis, plasma concentrations of melatonin and prolactin were not different between cows exposed to WLED or EIL. However, plasma cortisol concentration was lower (P < 0.01) for the EIL group (6.85 ± 4.49 ng/mL) than for the WLED group (23.9 ± 4.49 ng/mL) at dark period. Our results suggest that EIL exposure reduces stress experienced by cows as indicated by decreased cortisol concentration but did not affect lactational performance and plasma concentrations of melatonin and prolactin.
  5. 黒毛和種牛における耳標センサーの有用性, 村本　匠. 山野　晴樹. 田口　佑充. 金谷　晴一. 滝口　收. 衛藤　哲次. 塩塚　雄二. 藤野　亮一. 稲生　雄大. 髙橋　秀之., 第15回日本暖地畜産学会鹿児島大会, 2022年, 通常, 日本語
  6. 録音機を用いた音による行動検知, 山野　晴樹. 金谷　晴一. 滝口　收. 稲生　雄大. 衛藤　哲次. 塩塚　雄二. 藤野　亮一. 髙橋　秀之., 第15回日本暖地畜産学会鹿児島大会, 2022年, 通常, 日本語
  7. 代用乳多給が黒毛和種雌仔牛の繁殖成績および血液性状に及ぼす影響, 田口 佑充. 稲生 雄大. 早崎 幸輝. 前田 典之. 勘米良 善郎. 山崎 征二. 太田 昇. 武川 健司. 坪井 亜里沙. 宮本 浩邦. 衛藤 哲次. 塩塚 雄二. 藤野 亮一. 古瀬 充宏. 髙橋 秀之., 日本畜産学会第128回大会, 2021年, 通常, 日本語
  8. Effect of kraft pulp inclusion in calf starter on plasma concentration of glucagon-like peptide 2 in calves, Inabu Y, Kurosu K, Yasukawa Y, Hasunuma T, Ijima N, Funo H, Nishimura K, Kushibiki S, Kawashima K, Sugino T., International Symposium on Ruminant Physiology, 2019年, 通常, 英語, Glucagon-like peptide 2 (GLP-2), which plays a role in stimulation of intestinal growth, has been shown to be secreted in response to dietary fiber ingestion. Kraft pulp (KP), an intermediate product obtained when wood chips are converted to paper, contains high digestible neutral detergent fiber (NDF). The objective of this study was to evaluate the effect of KP inclusion in calf starter on plasma concentration of GLP-2 in calves. Holstein heifer calves (n = 25) were raised on a high plane of nutrition program using milk replacer [MR; 28.0 % crude protein (CP) and 15.0 % fat] until weaning at 49 d after birth. Calves were fed calf starter containing KP at 0 (CON; n = 14) or 12 % (KPS; n = 11) on a dry matter basis. Calf starters and timothy hay were offered ad libitum from the beginning of this study. All calf starters were formulated for 20.7% CP, and NDF content of CON- and KPS-starter were formulated for 16.4 and 22.6 %, respectively. Blood samples were collected at 4, 14, 21, 35, 49, 70, and 91 d after birth to measure plasma GLP-2 concentration. Data were analyzed by ANOVA of JMP® 14 using fit model procedure. Dry matter intake (DMI) of MR and calf starters did not differ among treatments, but hay DMI was lower (Treatment P = 0.02) for KPS (0.31 ± 0.06 kg/d) than for CON (0.44 ± 0.06 kg/d). Whereas, NDF intake was higher for KPS compared with CON from 56 to 84 d after birth (Treatment × time P < 0.01), which was due to lower NDF content for KPS caused by KP inclusion in calf staretr. Body weight and average daily gain were not affected by KP inclusion. Plasma GLP-2 concentration was not affected by dietary treatment at pre-weaning period but higher (Treatment P = 0.04) for KPS (0.6 ± 0.13 ng/mL) compared with CON (0.41 ± 0.13 ng/mL) at post-weaning period. These results indicate that KP inclusion in calf starter increases plasma GLP-2 concentration, which may be associated with greater fiber intake.
  9. Effects of feeding moderate- or high-starch close-up diet to cows on response of newborn calves to intravenous injection of glucagon-like peptide 1, Inabu Y, Haisan J, Oba M, Sugino T., ADSA Annual Meeting, 2019年, 通常, 英語, The objective of this study was to evaluate the effects of feeding moderate- or high-starch close-up diet to close-up cows on response of newborn calves to intravenously (i.v.) injected glucagon-like peptide 1 (GLP-1). Holstein heifer calves (n = 37) born to cows fed a moderate- (M, 14% starch; n = 17) or high-starch (H, 26% starch; n = 20) diet during the last 28 d of gestation were assigned to one of two treatment groups, which were i.v. injected with 5 mL of saline as control (MC and HC, n = 9 and 10, respectively) or GLP-1 solution (MG and HG, n = 8 and 10, respectively; at 1.0 μg/kg BW) immediately after milk replacer (MR) feeding finished (within 5 s after MR feeding finished) at 2, 10, and 20 days after birth. Blood samples were collected at −10, 0, 10, 20, 30, 40, 50, 60, 90, and 120 min relative to treatment injection and plasma glucose, insulin, and GLP-1 concentrations were measured. Data were analyzed by ANOVA using fit model procedure of JMP® 14 pro (SAS Institute Inc., Cary, NC). Plasma GLP-1 concentration increased rapidly after GLP-1 injection and was higher for the calves injected with GLP-1 than those injected with saline (P < 0.01) at 2 (1.97 vs. 1.02 ng/mL for G and C, respectively), 10 (2.10 vs. 0.67 ng/mL for G and C, respectively) and 20 days after birth (1.79 vs. 0.38 ng/mL for G and C, respectively), but no difference was observed between MG and HG at all sampling days. Both in M and H calves, the rise in postprandial plasma glucose concentration was suppressed (P < 0.01) by direct glucose-lowering action of i.v. injected GLP-1 at 10 (137 vs. 150 mg/dL for G and C, respectively) and 20 days after birth (147 vs. 158 mg/dL for G and C, respectively), and similar tendency was observed at 2 days after birth (133 vs. 144 mg/dL for G and C, respectively; P = 0.09): this direct glucose-lowering action by GLP-1 was greater (P = 0.02) for H than for M calves at 20 days after birth (141 vs. 152 mg/dL for HG and MG, respectively). These results indicate that feeding a high-starch diet to cows during close-up period enhances glucose-lowering action by GLP-1 after feeding depending on age of calves, which can affect glucose status in newborn calves.
  10. カーフスターターへのクラフトパルプ配合が子牛のグルカゴン様ペプチド2分泌に及ぼす影響, 稲生　雄大. 黒須　一博. 安川　結夏子. 蓮沼 俊哉. 飯島　紀美. 布野　秀忠. 西村　慶子. 櫛引　史郎. 川島　賢二. 杉野　利久., 日本畜産学会第126回大会, 2019年, 通常, 日本語
  11. 乾乳後期のエネルギー水準が新生子牛のグルカゴン様ペプチド１（GLP-1）を介したグルコース代謝に及ぼす影響, 稲生　雄大. Jennifer Haisan. 大場　真人. 杉野　利久., 日本畜産学会第125回大会, 2019年, 通常, 日本語
  12. Effects of pulse-dose intraruminal butyrate infusion on glucagon-like peptide 2 in dairy calves, Hatew B, Inabu Y, Sugino T, Steele M., ADSA Annual Meeting, 2018年, 通常, 英語
  13. Effect of extended colostrum feeding on plasma glucagon-like peptide 1 concentration in newborn calves, Inabu Y, Pyo J, Pletts S, Steele M A, Sugino T., ADSA Annual Meeting, 2018年, 通常, 英語, Glucagon-like peptide 1 (GLP-1) plays a role in the regulation of appetite and glucose homeostasis via the stimulation of insulin secretion. The objective of this study was to evaluate the effect of extended colostrum feeding on plasma concentrations of GLP-1. Holstein bull calves (n = 18) were fed pooled colostrum at 7.5% of BW at 2 h after birth, then fed mature milk (M), mixture at a ratio of 50:50 for pooled colostrum and milk (CM), or pooled colostrum (C; n = 6 for each treatment) at 5 % of BW at 12 h after birth, and every 12 h thereafter until 72 h after birth. Blood samples were obtained before (1 and 2 h after birth) and after (until 75 h after birth) the first colostrum feeding, and plasma concentrations of GLP-1, insulin and glucose were measured. Data were analyzed by ANOVA of JMP® 13 with treatment, time and treatment by time interaction as fixed effects. Treatment by time interaction was observed for plasma insulin and glucose concentrations (P < 0.01), which was mainly the result of lower concentrations from 14 to 27 h after birth (from 1 to 2 d after birth) for CM or C than for M. Conversely, on 3 d after birth, difference between treatments was not observed for insulin and glucose. Plasma GLP-1 concentration tended to be higher (P = 0.05) for C (2.26 ± 0.24 ng/mL; LSM ± SEM) compared with M (1.37 ± 0.24 ng/mL). A treatment by time interaction was observed for GLP-1 (P < 0.01), but unlike glucose and insulin, this was mainly the result of higher concentrations from 54 to 70 h after birth (3 d after birth) for C than for CM or M. Plasma concentration of glucose was not correlated with that of GLP-1 but positively correlated with that of insulin on d 1 (r = 0.48, P < 0.01) and d 2 (r = 0.49, P < 0.01) after birth. On 3 d after birth, plasma concentration of insulin was not correlated with that of glucose but positively correlated with that of GLP-1 (r = 0.34, P < 0.01). In conclusion, these results indicate that extended colostrum feeding may increase plasma GLP-1 concentrations, especially on 3 d after birth, but further study is necessary to determine the effect on plasma insulin and glucose concentrations.
  14. Pulse-dose intraruminal butyrate infusion increases plasma glucagon-like peptide 2 in dairy calves, Hatew B, Inabu Y, Steele M A., Western Canadian Dairy Seminar, 2018年, 通常, 英語
  15. Effect of extended colostrum feeding duration on gastrointestinal tract structural development of Holstein bull calves, Pyo J G, Pletts S I, Inabu Y, He Z, Haines D, Sugino T, Guan L L, Steele M A., Western Canadian Dairy Seminar, 2018年, 通常, 英語
  16. The effects of extended colostrum feeding on gastrointestinal tract growth of the neonatal dairy calf, Pyo J, Pletts S, Romao J, Inabu Y, He Z, Haines D, Sugino T, Guan L, Steele M., ASAS-CSAS Annual Meeting, 2018年, 通常, 英語
  17. Effect of delayed colostrum feeding on plasma concentrations of glucagon-like peptide 1 and 2 in calves, Inabu Y, Fischer A, Sugino T, Oba M, Guan L L, Steele M A., ADSA Annual Meeting, 2017年, 通常, 英語, Glucagon-like peptide 1 (GLP-1) plays a role in the regulation of appetite and glucose homeostasis via the stimulation of insulin secretion. The objective of this study was to evaluate the effect of extended colostrum feeding on plasma concentrations of GLP-1. Holstein bull calves (n = 18) were fed pooled colostrum at 7.5% of BW at 2 h after birth, then fed mature milk (M), mixture at a ratio of 50:50 for pooled colostrum and milk (CM), or pooled colostrum (C; n = 6 for each treatment) at 5 % of BW at 12 h after birth, and every 12 h thereafter until 72 h after birth. Blood samples were obtained before (1 and 2 h after birth) and after (until 75 h after birth) the first colostrum feeding, and plasma concentrations of GLP-1, insulin and glucose were measured. Data were analyzed by ANOVA of JMP® 13 with treatment, time and treatment by time interaction as fixed effects. Treatment by time interaction was observed for plasma insulin and glucose concentrations (P < 0.01), which was mainly the result of lower concentrations from 14 to 27 h after birth (from 1 to 2 d after birth) for CM or C than for M. Conversely, on 3 d after birth, difference between treatments was not observed for insulin and glucose. Plasma GLP-1 concentration tended to be higher (P = 0.05) for C (2.26 ± 0.24 ng/mL; LSM ± SEM) compared with M (1.37 ± 0.24 ng/mL). A treatment by time interaction was observed for GLP-1 (P < 0.01), but unlike glucose and insulin, this was mainly the result of higher concentrations from 54 to 70 h after birth (3 d after birth) for C than for CM or M. Plasma concentration of glucose was not correlated with that of GLP-1 but positively correlated with that of insulin on d 1 (r = 0.48, P < 0.01) and d 2 (r = 0.49, P < 0.01) after birth. On 3 d after birth, plasma concentration of insulin was not correlated with that of glucose but positively correlated with that of GLP-1 (r = 0.34, P < 0.01). In conclusion, these results indicate that extended colostrum feeding may increase plasma GLP-1 concentrations, especially on 3 d after birth, but further study is necessary to determine the effect on plasma insulin and glucose concentrations.
  18. 成雌ヤギへの初乳製剤経口投与がインスリン分泌に及ぼす影響, 杉野　利久. 大伴　清人. 松川　祥子. 稲生　雄大. 上野　和俊. 吉村　幸則. 小櫃　剛人. 磯部　直樹., 日本畜産学会第122回大会, 2017年, 通常, 日本語
  19. 離乳移行期におけるカーフスターターへのラクトース配合が子牛のグルカゴン様ペプチド2（GLP-2）分泌に及ぼす影響, 稲生　雄大. 三枝　亮仁. 猪内　勝利. 小池　聡. 小櫃　剛人. 大場　真人. 杉野　利久., 日本畜産学会第122回大会, 2017年, 通常, 日本語
  20. Effect of lactose inclusion in the calf starter on starter intake, growth performance and digestive organ development, Inouchi K, Saegusa A, Inabu Y, Sugino T, Oba M., 2016年, 通常, 英語, The objective of this study was to evaluate the effects of lactose inclusion in the calf starter on starter intake, growth performance and digestive organ development. Sixty Holstein bull calves were raised on the intensified nursing program using milk replacer containing 28% CP and 15% fat, and fed the texturized calf starter containing lactose at 0 (Control), 5.0 (LAC5), or 10.0% of dietary DM (LAC10; n = 20 for each treatment). All calf starters were formulated for 23.1% CP. As the pellet portion contained lactose and all adjusted ingredients, treatment calf starters differed only in the pellet. Ethanol soluble carbohydrate concentrations of Control, LAC5, and LAC10 were 7.3, 12.3 and 16.8%, respectively. Starch concentrations of Control, LAC5, and LAC10 were 29.7, 27.0 and 21.4%, respectively. All calves were fed treatment calf starters ad libitum, and their hay intake were limited to 150 g/d. Body weight, hip height, withers height, body length, hip width and heart girth were measured weekly. Fifteen calves were killed at the age of 62 d and 45 calves were killed at the age of 80 d. Digestive organs were harvested, emptied, rinsed and weighed. Starter dry matter intake was 267±45 (Control; mean±SE), 216±20 (LAC5), and 283±31 g/d (LAC10) before weaning (7-56 d), and 1516±156 (Control), 1344±105 (LAC5), and 1622±127 g/d (LAC10) during weaning transition (49-63 d), and 2778±164 (Control), 2636±109 (LAC5), and 2812±164 g/d (LAC10) after weaning (56-80 d). Average daily gain was 0.64±0.03 (Control), 0.64±0.03 (LAC5), and 0.71±0.34 kg/d (LAC10) before weaning (7-56 d), and 1.02±0.76 (Control), 1.03±0.08 (LAC5), and 1.17±0.08 kg/d (LAC10) during weaning transition (49-63 d), and 1.41±0.06 (Control), 1.40±0.06 (LAC5), and 1.34±0.06 kg/d (LAC10) after weaning (56-80 d). Wet mass of the reticulo-rumen was 1.37±0.14 (Control), 1.49±0.04 (LAC5), and 1.60±0.09%BW (LAC10)　at the age of 62 d, and 2.21±0.08 (Control), 2.03±0.07 (LAC5), and 1.97±0.16%BW (LAC10)　at the age of 80 d. None of the response variables above were statistically significant (P > 0.05). In addition, treatment did not affect the other primary response variables including body height, body length, heart girth, and wet mass of the other digestive organs. These results indicate that inclusion of lactose in the calf starter up to 10% of dietary DM may not affect starter intake, growth performance, and digestive organ development.
  21. Plasma concentrations of glucagon-like peptide 1 and 2 in calves fed calf starters containing lactose, Inabu Y, Saegusa A, Inouchi K, Oba M, Sugino T., ASAS-ADSA-CSAS-WSASAS Joint Annual Meeting, 2016年, 通常, 英語, The objective of this study was to evaluate the effect of lactose inclusion in calf starters on plasma concentrations of glucagon-like peptide 1 (GLP-1) and 2 (GLP-2). Holstein bull calves (n=60) were raised on an intensified nursing program using milk replacer containing 28.0 % CP and 15.0 % fat, and fed the texturized calf starter containing lactose at either 0 (Control), 5.0 (LAC5), or 10.0% (LAC10; n=20 for each treatment) on a DM basis. All calf starters were formulated for 23.1% CP. Ethanol soluble carbohydrate concentration of Control, LAC5 and LAC10 starters were 7.3, 12.3 and 16.8%, respectively. Starch concentrations of Control, LAC5 and LAC10 were 29.7, 27.0 and 21.4%, respectively. All calves were fed treatment calf starters ad libitum. Blood samples were obtained weekly from 1 wk to 11 wk of age and used to measure plasma GLP-1, GLP-2, insulin and β-hydroxyl butyric acid (BHB) concentrations. Plasma BHB concentrations were higher (P < 0.01) for LAC10 (169 ± 5.1 µmol/L; LSM ± SEM) compared with Control (153 ± 4.8 µmol/L) and LAC5 (148 ± 5.2 µmol/L). Plasma GLP-1 and GLP-2 concentrations were not affected by treatments. However, relative values of plasma GLP-1 concentrations compared with that of the baseline (1 wk of age) were higher (P < 0.01) for LAC10 (94.8 ± 5.01 %) compared with LAC5 (66.5 ± 5.11 %), and for LAC5 compared with Control (42.5 ± 4.73 %), and similar tendency was observed for GLP-2 concentrations relative to that of the baseline (80.6 ± 5.42 %, 74.7 ± 5.43 % and 73.3 ± 5.36 %, respectively for LAC10, LAC5, and Control, respectively; P = 0.09). Plasma insulin concentrations were lower (P < 0.01) for LAC5 (4.69 ± 0.58 ng/mL) and LAC10 (4.60 ± 0.58 ng/mL) compared with Control (5.52 ± 0.58 ng/mL). Lactose intake was positively correlated to plasma BHB concentrations (Spearman’s correlation coefficient; rs = 0.87, P < 0.01), and tended to be positively correlated to plasma GLP-1 concentrations (rs = 0.41, P = 0.07), but not correlated to plasma GLP-2 concentrations. In addition, plasma GLP-1 concentrations were positively correlated to plasma concentrations of BHB (rs = 0.85, P < 0.01). In conclusion, these results indicate that inclusion of lactose in calf starters may contribute to maintaining high plasma concentrations of GLP-1, which was associated with greater plasma BHB concentrations.
  22. Effect of lactose inclusion in calf starters on rumen fermentation of weaned calves, Saegusa A, Inouchi K, Ueno M, Inabu Y, Koike S, Sugino T, Oba M., ASAS-ADSA-CSAS-WSASAS Joint Annual Meeting, 2016年, 通常, 英語
  23. ヒツジにおけるグルカゴン様ペプチド2（GLP-2）分泌の日内変動, 石掛　以果. 稲生　雄大. 杉野　利久. 小櫃　剛人., 日本畜産学会第120回大会, 2015年, 通常, 日本語
  24. ヒツジにおけるVFAがグルカゴン様ペプチド2（GLP-2）分泌に及ぼす影響, 稲生　雄大. 杉野　利久. 小櫃　剛人. 石掛　以果, 日本畜産学会第120回大会, 2015年, 通常, 日本語, 酪農学園大学
  25. 哺乳期における抗菌薬無添加の代用乳給与が黒毛和種仔牛の成長に与える影響, 岡田 隼之介. 田口 祐充. 藤野 亮一. 塩塚 雄二. 衛藤 哲次. 稲生 雄大. 齋藤 昭. 髙橋 秀之., 日本畜産学会第128回大会, 通常, 日本語

受賞

  1. 日本畜産学会奨励賞第123号
  2. 日本畜産学会第125回大会優秀発表賞
  3. 平成30 年度広島大学エクセレントスチューデント
  4. 平成29 年度広島大学エクセレントスチューデント

外部資金

競争的資金等の採択状況

  1. 公益財団法人伊藤記念財団研究助成, 寒冷環境下における維持エネルギー量の増加を考慮した子牛の代用乳エネルギー含量の検討, 2024年
  2. 若手研究, 消化管内細菌による仔牛のグルカゴン様ペプチド2分泌制御機構の解明, 2024年, 2027年
  3. 公益財団法人伊藤記念財団研究助成, グルカゴン様ペプチド１に着目した仔牛のグルコース利用性向上, 2023年
  4. 若手研究, GLP-2分泌を刺激する酪酸を用いた新たな黒毛和種繁殖牛の飼養管理, 2020年, 2022年
  5. 特別研究員奨励費（DC2）, 子牛の栄養代謝機能発達に関する研究, 2019年, 2019年

社会活動

学術会議等の主催

  1. 若手研究者交流会（サイエンスナイト）, 2025年09月, 2025年09月

その他社会貢献活動（広大・部局主催含）

  1. 第9回広島大学酪農技術セミナー・第79回関西畜産学会広島大会ジョイントシンポジウム, 第9回 広島大学酪農技術セミナー, 広島大学生物生産学部, 2024年/11月, 2024年/11月, 運営参加・支援