YUDAI INABU

Last Updated :2026/02/10

Affiliations, Positions
Graduate School of Integrated Sciences for Life, Assistant Professor
E-mail
inabuhiroshima-u.ac.jp
Other Contact Details
1-4-4, Kagamiyama, Higashihiroshima, Japan
TEL : (+81) FAX : (+81)

Basic Information

Major Professional Backgrounds

  • 2019/04, 2019/09, Japan Society for the Promotion of Science, Special Postdoctoral Researcher（DC2)
  • 2019/10, 2023/09, Kyushu University, Assistant Professor
  • 2023/10/01, Hiroshima University, Graduate School of Integrated Sciences for Life, Assistant Professor

Educational Backgrounds

  • Hiroshima University, Graduate School of Biosphere Science , Japan, 2017/04, 2019/09
  • Hiroshima University, Graduate School of Biosphere Science , Japan, 2015/04, 2017/03
  • Hiroshima University, School of Applied Biological Science, Japan, 2011/04, 2015/03

Academic Degrees

  • Hiroshima University
  • Hiroshima University

Research Fields

  • Agricultural sciences;Animal life science;Animal production science

Research Keywords

  • Glucagon-like peptide
  • Endocrinology
  • Beef cows
  • Dairy cows

Affiliated Academic Societies

  • Warm Regional Society of Animal Science, Japan
  • American Dairy Science Association (ADSA)
  • Japanese Society of Animal Science

Educational Activity

Course in Charge

  1. 2025, Undergraduate Education, Intensive, Laboratory Work in General Physics
  2. 2025, Undergraduate Education, 3Term, Research Front of Bioresource Science
  3. 2025, Undergraduate Education, 4Term, Environmental Animal Physiology
  4. 2025, Undergraduate Education, 2Term, Reading of Foreign Literature in Applied Animal and Plant Schience
  5. 2025, Undergraduate Education, 2Term, Production System in Livestock
  6. 2025, Undergraduate Education, Intensive, Laboratory and Field Works in Animal Production II
  7. 2025, Undergraduate Education, Second Semester, Graduation Thesis I
  8. 2025, Undergraduate Education, First Semester, Graduation Thesis II
  9. 2025, Undergraduate Education, Second Semester, Graduation Thesis III
  10. 2025, Undergraduate Education, Intensive, (AIMS) Introduction Physiology of Domestic Animals
  11. 2025, Undergraduate Education, Intensive, Introductory Physiology of Domestic Animals
  12. 2025, Graduate Education (Doctoral Program) , 1Term, Research Plans in Life Science
  13. 2025, Graduate Education (Master's Program) , 3Term, Smart Livestock Farming

Research Activities

Academic Papers

  1. Maternal administration of octanoate, a medium-chain fatty acid, improves feed efficiency of Japanese black calves through influencing gut bacteriome structure, Scientific Reports, 15(33557), 20250929
    Link to Paper Search Results by DOI
  2. Effect of Nitrate Supplementation on Digestion, Rumen Fermentation, Plasma Metabolites, and Nitrogen Use in Sheep Fed a High-Forage Diet, ANIMAL SCIENCE JOURNAL, 97(1), 20260106
    Link to Paper Search Results by DOI
  3. Comparison of milk production and endocrine profiles of dairy cows exposed to either white light-emitting diode or induction lighting, Domestic Animal Endocrinology, 93(106958), 20250613
    Link to Paper Search Results by DOI
  4. ★, Effect of feeding sodium butyrate to beef female cows during pre- and post-partum period on concentrations of glucagon-like peptides in plasma and colostrum, ANIMAL SCIENCE JOURNAL, 95(1), 202401
    Link to Paper Search Results by DOI
  5. Compost fermented with thermophilic Bacillaceae reduces heat stress–induced mortality in laying hens through gut microbial modulation, Animal Microbiome, 8(9), 20260203
    Link to Paper Search Results by DOI
  6. Effect of Ca-octanoate supplementation on concentrations of ghrelin and ghrelin-related hormones in plasma and milk of beef cattle, Heliyon, 9(6), e16795, 202306
  7. Estimation of silent phenotypes of calf antibiotic dysbiosis, Scientific Reports, 13, 6359, 202304
  8. Effect of kraft pulp inclusion in calf starter on performance, health, and plasma concentration of glucagon-like peptide 2 in calves, Journal of Dairy Science, 106(6), 4443-4453, 202306
  9. Development of a novel feeding method for Japanese black calves with thermophile probiotics at postweaning, Journal of Applied Microbiology, 132(5), 3870-3882, 202203
  10. Rumen microbiota and its relation to fermentation in lactose-fed calves, Journal of Dairy Science, 104(10), 10744-10752, 202110
  11. Glucagon-like peptide 2 (GLP-2) in bovine colostrum and transition milk, Heliyon, 7(5), e07046, 202105
  12. Effects of starch concentration of close-up diets on rumen pH and plasma metabolite responses of dairy cows to grain challenges after calving, Journal of Dairy Science, 103(12), 11461-11471, 202012
  13. Effects of feeding high volumes of milk replacer on reproductive performance and on concentrations of metabolites and hormones in blood of Japanese black heifer calves, Animal Science Journal, 92(1), e13505, 202101
  14. Effects of pre- and postpartum dietary starch content on productivity, plasma energy metabolites, and serum inflammation indicators of dairy cows, Journal of Dairy Science, 104(4), 4362-4374, 202104
  15. ★, Effects of feeding a moderate- or high-energy close-up diet to cows on response of newborn calves to milk replacer feeding and intravenous injection of glucagon-like peptide 1, Domestic Animal Endocrinology, 74(106528), 202101
  16. Feeding colostrum or a 1:1 colostrum:milk mixture for 3 days postnatal increases small intestinal development and minimally influences plasma glucagon-like peptide-2 and serum insulin-like growth factor-1 concentrations in Holstein bull calves, Journal of Dairy Science, 103(5), 4236-4251, 202003
  17. Effects of feeding a high- or moderate-starch prepartum diet to cows on newborn dairy heifer calf responses to intravenous glucose tolerance tests early in life, Journal of Dairy Science, 102(10), 8931-8940, 201910
  18. The effect of tributyrin supplementation to milk replacer on plasma glucagon-like peptide 2 concentrations in pre-weaning calves, Animal Science Journal, 90(9), 1185-1192, 201909
  19. ★, Effect of extended colostrum feeding on plasma glucagon-like peptide-1 concentration in newborn calves, Journal of Dairy Science, 102(5), 4619-4627, 201903
  20. Effects of pulse-dose ruminal infusion of butyrate on plasma glucagon-like peptide 1 and 2 concentrations in dairy calves, Journal of Dairy Science, 102(3), 2254-2265, 201903
  21. ★, Short communication: The effect of delayed colostrum feeding on plasma concentrations of glucagon-like peptide 1 and 2 in newborn calves, Journal of Dairy Science, 101(7), 6627-6631, 201807
  22. ★, Plasma concentrations of glucagon-like peptide 1 and 2 in calves fed calf starters containing lactose, Journal of Dairy Science, 100(11), 9361-9371, 201709
  23. Response of plasma glucagon-like peptide-2 to feeding pattern and intraruminal administration of volatile fatty acids in sheep, Domestic Animal Endocrinology, 60, 31-41, 201707
  24. Effects of partial replacement of corn grain with lactose in calf starters on ruminal fermentation and growth performance, Journal of Dairy Science, 100(8), 6177-6186, 201708

Invited Lecture, Oral Presentation, Poster Presentation

  1. Effects of Butyric Acid Supplementation on Glucagon-Like Peptide-1 Secretion and Glucose Metabolism in Dairy Calves, Yudai Inabu, Taro Tsugumune, Toshihisa Sugino, Taketo Obitsu, 2025/09, Without Invitation, Japanese
  2. Effect of feeding sodium butyrate to beef female cows during calving transition period on glucagon-like peptide 1 and 2 concentrations in colostrum and transition milk., Yudai Inabu, Hiroshi Horike, Haruki Yamano, Yutaka Taguchi, Shunnosuke Okada, Tetsuji Etoh, Yuji Shiotsuka, Ryoichi Fujino, Hideyuki Takahashi, 2024/09, Without Invitation, Japanese
  3. Effect of feeding sodium butyrate to beef female cows during calving transition period on glucagon-like peptide 1 and 2 concentrations in colostrum and transition milk., Y. Inabu, H. Horike, H. Yamano, Y. Taguchi, S. Okada, T. Etoh, Y. Shiotsuka, R. Fujino, H. Takahashi, H. Horike, H. Yamano, Y. Taguchi, S. Okada, T. Etoh, Y. Shiotsuka, R. Fujino, H. Takahashi, International Symposium on Ruminant Physiology (ISRP), 2024, Without Invitation, English, Chicago, Secretion of glucagon-like peptide (GLP)-1 and 2 (GLP-2), a gut-derived peptide secreted from intestinal L-cells, is potently stimulated by dietary butyrate provision. Since the hormones contained in milk are transferred from the maternal blood circulation, an increase in circulating levels of GLP-1 and 2 due to dietary butyrate could result in increased levels in colostrum and transition milk. This study evaluated the effect of feeding beef cows with sodium butyrate (SB) during the late pregnancy and early post-partum periods on concentrations of GLP-1 and 2 in plasma, colostrum, and transition milk. Twelve Japanese Black female cows were fed hay and concentrate according to Japanese feeding standard for beef cows (2008) with (BUTY) or without (CON; n = 6 for each treatment) SB supplementation at 1.5% of the dietary dry matter from 60 d before the expected calving date (−60 d) to 4 d after calving. Blood samples were collected 1 h before feeding on −60, −30, and −7 d, and 0 (calving date), 1, 2, and 3 d after calving. Milk samples were collected immediately after blood sampling post-partum. Data were analyzed by ANOVA of JMP® 14 using fit model procedure. Plasma total cholesterol concentration was higher (P = 0.04) for the BUTY (151 ± 6.27 mg/dL) than for the CON (131 ± 6.27 mg/dL). In addition, plasma GLP-1 concentration was higher for the BUTY (0.82 ± 0.092 ng/mL) than for the CON (0.50 ± 0.092) at 3 d after calving (P < 0.05). On the other hand, plasma GLP-2 concentration was not affected by treatment. This study showed for the first time that GLP-1 and 2 were present in bovine colostrum at higher concentrations as compared to in plasma at −7 and 0 d (P < 0.01). On the other hand, treatment did not affect metabolite and hormone concentrations in colostrum and transition milk. In summary, feeding beef cows with SB during the calving transition period likely increases plasma GLP-1 concentrations post-partum without affecting the components of colostrum and transition milk.
  4. Effects of exposure to white LED or electrodeless induction lamp on milk production performance and blood compositions of dairy cows, Inabu Y, Takakura Y, Shinohara Y, Sunadome M, Watanabe R, Kushibiki S, Obitsu T, Sugino T., ADSA Annual Meeting, 2024, Without Invitation, English, Electrodeless induction lamp (EIL) provides lower blue light as compared to white LED (WLED) and are now widely used in various facilities. The objective of this study was to evaluate the response of milk production and physiological parameters to exposure to WLED or EIL in Holstein cows. Ten Holstein lactating cows (225 ± 32.5 DIM, 710 ± 24.6 kg initial BW, 2.56 ± 1.59 parity) were managed under the long-day photoperiod (16:8 h light-dark cycle) and were assigned to two treatments for 3 weeks each in a 2 by 2 crossover design as follows: exposure to WLED (453 nm peak wavelength, 231 Lux) or EIL (550nm peak wavelength, 237 Lux) during daytime. The dark period (21:00 h to 5:00 h) for all treatments was no illumination (0.0 lux). All cows were fed same TMR ad libitum throughout the experiment. Milk samples were collected every week to measure milk compositions. Serial blood sampling was performed on the last day of each treatment to measure metabolite and hormone concentrations in plasma. Data were analyzed by ANOVA using Fit Model procedure of JMP® 17 pro (SAS Institute Inc., Cary, NC). Dry matter intake, BW, milk yield and composition, rumination time and plasma metabolite concentrations were not affected by light treatment. Contrary to our hypothesis, plasma concentrations of melatonin and prolactin were not different between cows exposed to WLED or EIL. However, plasma cortisol concentration was lower (P < 0.01) for the EIL group (6.85 ± 4.49 ng/mL) than for the WLED group (23.9 ± 4.49 ng/mL) at dark period. Our results suggest that EIL exposure reduces stress experienced by cows as indicated by decreased cortisol concentration but did not affect lactational performance and plasma concentrations of melatonin and prolactin.
  5. Effect of kraft pulp inclusion in calf starter on plasma concentration of glucagon-like peptide 2 in calves, Inabu Y, Kurosu K, Yasukawa Y, Hasunuma T, Ijima N, Funo H, Nishimura K, Kushibiki S, Kawashima K, Sugino T., International Symposium on Ruminant Physiology, 2019, Without Invitation, English, Glucagon-like peptide 2 (GLP-2), which plays a role in stimulation of intestinal growth, has been shown to be secreted in response to dietary fiber ingestion. Kraft pulp (KP), an intermediate product obtained when wood chips are converted to paper, contains high digestible neutral detergent fiber (NDF). The objective of this study was to evaluate the effect of KP inclusion in calf starter on plasma concentration of GLP-2 in calves. Holstein heifer calves (n = 25) were raised on a high plane of nutrition program using milk replacer [MR; 28.0 % crude protein (CP) and 15.0 % fat] until weaning at 49 d after birth. Calves were fed calf starter containing KP at 0 (CON; n = 14) or 12 % (KPS; n = 11) on a dry matter basis. Calf starters and timothy hay were offered ad libitum from the beginning of this study. All calf starters were formulated for 20.7% CP, and NDF content of CON- and KPS-starter were formulated for 16.4 and 22.6 %, respectively. Blood samples were collected at 4, 14, 21, 35, 49, 70, and 91 d after birth to measure plasma GLP-2 concentration. Data were analyzed by ANOVA of JMP® 14 using fit model procedure. Dry matter intake (DMI) of MR and calf starters did not differ among treatments, but hay DMI was lower (Treatment P = 0.02) for KPS (0.31 ± 0.06 kg/d) than for CON (0.44 ± 0.06 kg/d). Whereas, NDF intake was higher for KPS compared with CON from 56 to 84 d after birth (Treatment × time P < 0.01), which was due to lower NDF content for KPS caused by KP inclusion in calf staretr. Body weight and average daily gain were not affected by KP inclusion. Plasma GLP-2 concentration was not affected by dietary treatment at pre-weaning period but higher (Treatment P = 0.04) for KPS (0.6 ± 0.13 ng/mL) compared with CON (0.41 ± 0.13 ng/mL) at post-weaning period. These results indicate that KP inclusion in calf starter increases plasma GLP-2 concentration, which may be associated with greater fiber intake.
  6. Effects of feeding moderate- or high-starch close-up diet to cows on response of newborn calves to intravenous injection of glucagon-like peptide 1, Inabu Y, Haisan J, Oba M, Sugino T., ADSA Annual Meeting, 2019, Without Invitation, English, The objective of this study was to evaluate the effects of feeding moderate- or high-starch close-up diet to close-up cows on response of newborn calves to intravenously (i.v.) injected glucagon-like peptide 1 (GLP-1). Holstein heifer calves (n = 37) born to cows fed a moderate- (M, 14% starch; n = 17) or high-starch (H, 26% starch; n = 20) diet during the last 28 d of gestation were assigned to one of two treatment groups, which were i.v. injected with 5 mL of saline as control (MC and HC, n = 9 and 10, respectively) or GLP-1 solution (MG and HG, n = 8 and 10, respectively; at 1.0 μg/kg BW) immediately after milk replacer (MR) feeding finished (within 5 s after MR feeding finished) at 2, 10, and 20 days after birth. Blood samples were collected at −10, 0, 10, 20, 30, 40, 50, 60, 90, and 120 min relative to treatment injection and plasma glucose, insulin, and GLP-1 concentrations were measured. Data were analyzed by ANOVA using fit model procedure of JMP® 14 pro (SAS Institute Inc., Cary, NC). Plasma GLP-1 concentration increased rapidly after GLP-1 injection and was higher for the calves injected with GLP-1 than those injected with saline (P < 0.01) at 2 (1.97 vs. 1.02 ng/mL for G and C, respectively), 10 (2.10 vs. 0.67 ng/mL for G and C, respectively) and 20 days after birth (1.79 vs. 0.38 ng/mL for G and C, respectively), but no difference was observed between MG and HG at all sampling days. Both in M and H calves, the rise in postprandial plasma glucose concentration was suppressed (P < 0.01) by direct glucose-lowering action of i.v. injected GLP-1 at 10 (137 vs. 150 mg/dL for G and C, respectively) and 20 days after birth (147 vs. 158 mg/dL for G and C, respectively), and similar tendency was observed at 2 days after birth (133 vs. 144 mg/dL for G and C, respectively; P = 0.09): this direct glucose-lowering action by GLP-1 was greater (P = 0.02) for H than for M calves at 20 days after birth (141 vs. 152 mg/dL for HG and MG, respectively). These results indicate that feeding a high-starch diet to cows during close-up period enhances glucose-lowering action by GLP-1 after feeding depending on age of calves, which can affect glucose status in newborn calves.
  7. Effects of pulse-dose intraruminal butyrate infusion on glucagon-like peptide 2 in dairy calves, Hatew B, Inabu Y, Sugino T, Steele M., ADSA Annual Meeting, 2018, Without Invitation, English
  8. Effect of extended colostrum feeding on plasma glucagon-like peptide 1 concentration in newborn calves, Inabu Y, Pyo J, Pletts S, Steele M A, Sugino T., ADSA Annual Meeting, 2018, Without Invitation, English, Glucagon-like peptide 1 (GLP-1) plays a role in the regulation of appetite and glucose homeostasis via the stimulation of insulin secretion. The objective of this study was to evaluate the effect of extended colostrum feeding on plasma concentrations of GLP-1. Holstein bull calves (n = 18) were fed pooled colostrum at 7.5% of BW at 2 h after birth, then fed mature milk (M), mixture at a ratio of 50:50 for pooled colostrum and milk (CM), or pooled colostrum (C; n = 6 for each treatment) at 5 % of BW at 12 h after birth, and every 12 h thereafter until 72 h after birth. Blood samples were obtained before (1 and 2 h after birth) and after (until 75 h after birth) the first colostrum feeding, and plasma concentrations of GLP-1, insulin and glucose were measured. Data were analyzed by ANOVA of JMP® 13 with treatment, time and treatment by time interaction as fixed effects. Treatment by time interaction was observed for plasma insulin and glucose concentrations (P < 0.01), which was mainly the result of lower concentrations from 14 to 27 h after birth (from 1 to 2 d after birth) for CM or C than for M. Conversely, on 3 d after birth, difference between treatments was not observed for insulin and glucose. Plasma GLP-1 concentration tended to be higher (P = 0.05) for C (2.26 ± 0.24 ng/mL; LSM ± SEM) compared with M (1.37 ± 0.24 ng/mL). A treatment by time interaction was observed for GLP-1 (P < 0.01), but unlike glucose and insulin, this was mainly the result of higher concentrations from 54 to 70 h after birth (3 d after birth) for C than for CM or M. Plasma concentration of glucose was not correlated with that of GLP-1 but positively correlated with that of insulin on d 1 (r = 0.48, P < 0.01) and d 2 (r = 0.49, P < 0.01) after birth. On 3 d after birth, plasma concentration of insulin was not correlated with that of glucose but positively correlated with that of GLP-1 (r = 0.34, P < 0.01). In conclusion, these results indicate that extended colostrum feeding may increase plasma GLP-1 concentrations, especially on 3 d after birth, but further study is necessary to determine the effect on plasma insulin and glucose concentrations.
  9. Pulse-dose intraruminal butyrate infusion increases plasma glucagon-like peptide 2 in dairy calves, Hatew B, Inabu Y, Steele M A., Western Canadian Dairy Seminar, 2018, Without Invitation, English
  10. Effect of extended colostrum feeding duration on gastrointestinal tract structural development of Holstein bull calves, Pyo J G, Pletts S I, Inabu Y, He Z, Haines D, Sugino T, Guan L L, Steele M A., Western Canadian Dairy Seminar, 2018, Without Invitation, English
  11. The effects of extended colostrum feeding on gastrointestinal tract growth of the neonatal dairy calf, Pyo J, Pletts S, Romao J, Inabu Y, He Z, Haines D, Sugino T, Guan L, Steele M., ASAS-CSAS Annual Meeting, 2018, Without Invitation, English
  12. Effect of delayed colostrum feeding on plasma concentrations of glucagon-like peptide 1 and 2 in calves, Inabu Y, Fischer A, Sugino T, Oba M, Guan L L, Steele M A., ADSA Annual Meeting, 2017, Without Invitation, English, Glucagon-like peptide 1 (GLP-1) plays a role in the regulation of appetite and glucose homeostasis via the stimulation of insulin secretion. The objective of this study was to evaluate the effect of extended colostrum feeding on plasma concentrations of GLP-1. Holstein bull calves (n = 18) were fed pooled colostrum at 7.5% of BW at 2 h after birth, then fed mature milk (M), mixture at a ratio of 50:50 for pooled colostrum and milk (CM), or pooled colostrum (C; n = 6 for each treatment) at 5 % of BW at 12 h after birth, and every 12 h thereafter until 72 h after birth. Blood samples were obtained before (1 and 2 h after birth) and after (until 75 h after birth) the first colostrum feeding, and plasma concentrations of GLP-1, insulin and glucose were measured. Data were analyzed by ANOVA of JMP® 13 with treatment, time and treatment by time interaction as fixed effects. Treatment by time interaction was observed for plasma insulin and glucose concentrations (P < 0.01), which was mainly the result of lower concentrations from 14 to 27 h after birth (from 1 to 2 d after birth) for CM or C than for M. Conversely, on 3 d after birth, difference between treatments was not observed for insulin and glucose. Plasma GLP-1 concentration tended to be higher (P = 0.05) for C (2.26 ± 0.24 ng/mL; LSM ± SEM) compared with M (1.37 ± 0.24 ng/mL). A treatment by time interaction was observed for GLP-1 (P < 0.01), but unlike glucose and insulin, this was mainly the result of higher concentrations from 54 to 70 h after birth (3 d after birth) for C than for CM or M. Plasma concentration of glucose was not correlated with that of GLP-1 but positively correlated with that of insulin on d 1 (r = 0.48, P < 0.01) and d 2 (r = 0.49, P < 0.01) after birth. On 3 d after birth, plasma concentration of insulin was not correlated with that of glucose but positively correlated with that of GLP-1 (r = 0.34, P < 0.01). In conclusion, these results indicate that extended colostrum feeding may increase plasma GLP-1 concentrations, especially on 3 d after birth, but further study is necessary to determine the effect on plasma insulin and glucose concentrations.
  13. Effect of lactose inclusion in the calf starter on starter intake, growth performance and digestive organ development, Inouchi K, Saegusa A, Inabu Y, Sugino T, Oba M., ASAS-ADSA-CSAS-WSASAS Joint Annual Meeting, 2016, Without Invitation, English, The objective of this study was to evaluate the effects of lactose inclusion in the calf starter on starter intake, growth performance and digestive organ development. Sixty Holstein bull calves were raised on the intensified nursing program using milk replacer containing 28% CP and 15% fat, and fed the texturized calf starter containing lactose at 0 (Control), 5.0 (LAC5), or 10.0% of dietary DM (LAC10; n = 20 for each treatment). All calf starters were formulated for 23.1% CP. As the pellet portion contained lactose and all adjusted ingredients, treatment calf starters differed only in the pellet. Ethanol soluble carbohydrate concentrations of Control, LAC5, and LAC10 were 7.3, 12.3 and 16.8%, respectively. Starch concentrations of Control, LAC5, and LAC10 were 29.7, 27.0 and 21.4%, respectively. All calves were fed treatment calf starters ad libitum, and their hay intake were limited to 150 g/d. Body weight, hip height, withers height, body length, hip width and heart girth were measured weekly. Fifteen calves were killed at the age of 62 d and 45 calves were killed at the age of 80 d. Digestive organs were harvested, emptied, rinsed and weighed. Starter dry matter intake was 267±45 (Control; mean±SE), 216±20 (LAC5), and 283±31 g/d (LAC10) before weaning (7-56 d), and 1516±156 (Control), 1344±105 (LAC5), and 1622±127 g/d (LAC10) during weaning transition (49-63 d), and 2778±164 (Control), 2636±109 (LAC5), and 2812±164 g/d (LAC10) after weaning (56-80 d). Average daily gain was 0.64±0.03 (Control), 0.64±0.03 (LAC5), and 0.71±0.34 kg/d (LAC10) before weaning (7-56 d), and 1.02±0.76 (Control), 1.03±0.08 (LAC5), and 1.17±0.08 kg/d (LAC10) during weaning transition (49-63 d), and 1.41±0.06 (Control), 1.40±0.06 (LAC5), and 1.34±0.06 kg/d (LAC10) after weaning (56-80 d). Wet mass of the reticulo-rumen was 1.37±0.14 (Control), 1.49±0.04 (LAC5), and 1.60±0.09%BW (LAC10)　at the age of 62 d, and 2.21±0.08 (Control), 2.03±0.07 (LAC5), and 1.97±0.16%BW (LAC10)　at the age of 80 d. None of the response variables above were statistically significant (P > 0.05). In addition, treatment did not affect the other primary response variables including body height, body length, heart girth, and wet mass of the other digestive organs. These results indicate that inclusion of lactose in the calf starter up to 10% of dietary DM may not affect starter intake, growth performance, and digestive organ development.
  14. Plasma concentrations of glucagon-like peptide 1 and 2 in calves fed calf starters containing lactose, Inabu Y, Saegusa A, Inouchi K, Oba M, Sugino T., ASAS-ADSA-CSAS-WSASAS Joint Annual Meeting, 2016, Without Invitation, English, The objective of this study was to evaluate the effect of lactose inclusion in calf starters on plasma concentrations of glucagon-like peptide 1 (GLP-1) and 2 (GLP-2). Holstein bull calves (n=60) were raised on an intensified nursing program using milk replacer containing 28.0 % CP and 15.0 % fat, and fed the texturized calf starter containing lactose at either 0 (Control), 5.0 (LAC5), or 10.0% (LAC10; n=20 for each treatment) on a DM basis. All calf starters were formulated for 23.1% CP. Ethanol soluble carbohydrate concentration of Control, LAC5 and LAC10 starters were 7.3, 12.3 and 16.8%, respectively. Starch concentrations of Control, LAC5 and LAC10 were 29.7, 27.0 and 21.4%, respectively. All calves were fed treatment calf starters ad libitum. Blood samples were obtained weekly from 1 wk to 11 wk of age and used to measure plasma GLP-1, GLP-2, insulin and β-hydroxyl butyric acid (BHB) concentrations. Plasma BHB concentrations were higher (P < 0.01) for LAC10 (169 ± 5.1 µmol/L; LSM ± SEM) compared with Control (153 ± 4.8 µmol/L) and LAC5 (148 ± 5.2 µmol/L). Plasma GLP-1 and GLP-2 concentrations were not affected by treatments. However, relative values of plasma GLP-1 concentrations compared with that of the baseline (1 wk of age) were higher (P < 0.01) for LAC10 (94.8 ± 5.01 %) compared with LAC5 (66.5 ± 5.11 %), and for LAC5 compared with Control (42.5 ± 4.73 %), and similar tendency was observed for GLP-2 concentrations relative to that of the baseline (80.6 ± 5.42 %, 74.7 ± 5.43 % and 73.3 ± 5.36 %, respectively for LAC10, LAC5, and Control, respectively; P = 0.09). Plasma insulin concentrations were lower (P < 0.01) for LAC5 (4.69 ± 0.58 ng/mL) and LAC10 (4.60 ± 0.58 ng/mL) compared with Control (5.52 ± 0.58 ng/mL). Lactose intake was positively correlated to plasma BHB concentrations (Spearman’s correlation coefficient; rs = 0.87, P < 0.01), and tended to be positively correlated to plasma GLP-1 concentrations (rs = 0.41, P = 0.07), but not correlated to plasma GLP-2 concentrations. In addition, plasma GLP-1 concentrations were positively correlated to plasma concentrations of BHB (rs = 0.85, P < 0.01). In conclusion, these results indicate that inclusion of lactose in calf starters may contribute to maintaining high plasma concentrations of GLP-1, which was associated with greater plasma BHB concentrations.
  15. Effect of lactose inclusion in calf starters on rumen fermentation of weaned calves, Saegusa A, Inouchi K, Ueno M, Inabu Y, Koike S, Sugino T, Oba M., ASAS-ADSA-CSAS-WSASAS Joint Annual Meeting, 2016, Without Invitation, English

External Funds

Acceptance Results of Competitive Funds

  1. 2024
  2. 2024, 2027
  3. 2023
  4. 2020, 2022
  5. 2019, 2019