渡邉 朋信TOMONOBU WATANABE

Last Updated :2022/11/01

所属・職名
原爆放射線医科学研究所 教授
メールアドレス
twatanabehiroshima-u.ac.jp

基本情報

学位

  • 修士(理学) (大阪大学)
  • 博士(理学) (大阪大学)

教育活動

授業担当

  1. 2022年, 学部専門, 集中, 医学研究実習
  2. 2022年, 修士課程・博士課程前期, セメスター(前期), 特別演習
  3. 2022年, 修士課程・博士課程前期, セメスター(後期), 特別演習
  4. 2022年, 修士課程・博士課程前期, セメスター(前期), 特別研究
  5. 2022年, 修士課程・博士課程前期, セメスター(後期), 特別研究
  6. 2022年, 博士課程・博士課程後期, セメスター(前期), 特別演習
  7. 2022年, 博士課程・博士課程後期, セメスター(後期), 特別演習
  8. 2022年, 博士課程・博士課程後期, セメスター(前期), 特別研究
  9. 2022年, 博士課程・博士課程後期, セメスター(後期), 特別研究
  10. 2022年, 博士課程・博士課程後期, セメスター(前期), 幹細胞機能学特別演習
  11. 2022年, 博士課程・博士課程後期, セメスター(後期), 幹細胞機能学特別演習
  12. 2022年, 博士課程・博士課程後期, セメスター(前期), 幹細胞機能学特別研究
  13. 2022年, 博士課程・博士課程後期, セメスター(後期), 幹細胞機能学特別研究
  14. 2022年, 修士課程・博士課程前期, 3ターム, 生命医科学セミナー A
  15. 2022年, 修士課程・博士課程前期, 3ターム, 生命医科学セミナー B
  16. 2022年, 博士課程・博士課程後期, 3ターム, 生命医科学セミナーC
  17. 2022年, 博士課程・博士課程後期, 3ターム, 生命医科学セミナーD
  18. 2022年, 博士課程・博士課程後期, 3ターム, 生命医科学セミナーE

研究活動

学術論文(★は代表的な論文)

  1. Mitochondria-associated myosin 19 processively transports mitochondria on actin tracks in living cells, Journal of Biological Chemistry, 20220320
  2. Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration, ISCIENCE, 25巻, 7号, 20220715
  3. Valosin-containing protein regulates the stability of fused in sarcoma granules in cells by changing ATP concentrations, FEBS LETTERS, 596巻, 11号, pp. 1412-1423, 202206
  4. Non-label immune cell state prediction using Raman spectroscopy., Scientific reports, 6巻, 2016
  5. Single- and multiple-molecule dynamics of the signaling from H-Ras to cRaf-1 visualized on the plasma membrane of living cells., Chemphyschem : a European journal of chemical physics and physical chemistry, 4巻, 7号, 2003
  6. A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successively., Proceedings of the National Academy of Sciences of the United States of America, 101巻, 26号, 2004
  7. Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein., Journal of cell science, 119巻, Pt 6号, 2006
  8. Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein., Proceedings of the National Academy of Sciences of the United States of America, 103巻, 15号, 2006
  9. Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining., Biochemical and biophysical research communications, 351巻, 1号, 2006
  10. Input-output relationship in galvanotactic response of Dictyostelium cells., Bio Systems, 88巻, 3号, 2007
  11. Stepwise movements in vesicle transport of HER2 by motor proteins in living cells., Biophysical journal, 92巻, 11号, 2007
  12. Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics., Biochemical and biophysical research communications, 359巻, 1号, 2007
  13. Human myosin Vc is a low duty ratio nonprocessive motor., The Journal of biological chemistry, 283巻, 16号, 2008
  14. In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dots., The Journal of biological chemistry, 285巻, 4号, 2010
  15. Myosin-X induces filopodia by multiple elongation mechanism., The Journal of biological chemistry, 285巻, 25号, 2010
  16. Single molecular observation of self-regulated kinesin motility., Biochemistry, 49巻, 22号, 2010
  17. Identification of three distinct functional sites of insulin-mediated GLUT4 trafficking in adipocytes using quantitative single molecule imaging., Molecular biology of the cell, 21巻, 15号, 2010
  18. Mechanical characterization of one-headed myosin-V using optical tweezers., PloS one, 5巻, 8号, 2010
  19. Antibody-protein A conjugated quantum dots for multiplexed imaging of surface receptors in living cells., Molecular bioSystems, 6巻, 11号, 2010
  20. Real-time nanoscopy by using blinking enhanced quantum dots., Biophysical journal, 99巻, 7号, 2010
  21. Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells., Sensors (Basel, Switzerland), 9巻, 11号, 2009
  22. Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts., Biochemical and biophysical research communications, 418巻, 4号, 2012
  23. Engineering strain-sensitive yellow fluorescent protein., Chemical communications (Cambridge, England), 48巻, 63号, 2012
  24. Atg9 vesicles are an important membrane source during early steps of autophagosome formation., The Journal of cell biology, 198巻, 2号, 2012
  25. Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors., Biomaterials, 33巻, 33号, 2012
  26. Distinct modulated pupil function system for real-time imaging of living cells., PloS one, 7巻, 9号, 2012
  27. Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging., Proceedings of the National Academy of Sciences of the United States of America, 110巻, 9号, 2013
  28. Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement., Nature communications, 4巻, 2013
  29. Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods., Biophysical journal, 105巻, 3号, 2013
  30. Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure., PloS one, 8巻, 8号, 2013
  31. Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers., Journal of bioscience and bioengineering, 117巻, 6号, 2014
  32. Visualizing cell state transition using Raman spectroscopy., PloS one, 9巻, 1号, 2014
  33. Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis., Cell, 157巻, 3号, 2014
  34. Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles., Frontiers in physiology, 5巻, 2014
  35. Gene dynamics of core transcription factors for pluripotency in embryonic stem cells., Journal of bioscience and bioengineering, 119巻, 4号, 2015
  36. Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging., Proceedings of the National Academy of Sciences of the United States of America, 112巻, 14号, 2015
  37. Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging., Scientific reports, 5巻, 2015
  38. SH3 domain of c-Src governs its dynamics at focal adhesions and the cell membrane., The FEBS journal, 282巻, 20号, 2015
  39. Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging., Scientific reports, 5巻, 2015
  40. Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy., Applied optics, 55巻, 5号, 2016
  41. Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it., Scientific reports, 6巻, 2016
  42. Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy: erratum., Applied optics, 55巻, 15号, 2016
  43. Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots., Biomedical optics express, 7巻, 7号, 2016
  44. Activated full-length myosin-X moves processively on filopodia with large steps toward diverse two-dimensional directions., Scientific reports, 7巻, 2017
  45. Human myosin VIIa is a very slow processive motor protein on various cellular actin structures., The Journal of biological chemistry, 292巻, 26号, 2017
  46. Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters., Biophysical reviews, 8巻, 2号, 2016
  47. A novel c-Src recruitment pathway from the cytosol to focal adhesions., FEBS letters, 591巻, 13号, 2017
  48. Raman spectroscopy as a tool for ecology and evolution., Journal of the Royal Society, Interface, 14巻, 131号, 2017
  49. In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis., Scientific reports, 7巻, 1号, 2017
  50. The use of a genetically encoded molecular crowding sensor in various biological phenomena., Biophysics and physicobiology, 14巻, 2017
  51. Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation., Scientific reports, 7巻, 1号, 2017
  52. Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process., Scientific reports, 8巻, 1号, 2018
  53. Cell type discrimination based on image features of molecular component distribution., Scientific reports, 8巻, 1号, 2018
  54. Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation., Scientific reports, 8巻, 1号, 2018
  55. Raman spectral signature reflects transcriptomic features of antibiotic resistance in Escherichia coli., Communications biology, 1巻, 2018
  56. Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport., The Journal of cell biology, 217巻, 12号, 2018
  57. Single-Cell Screening of Tamoxifen Abundance and Effect Using Mass Spectrometry and Raman-Spectroscopy., Analytical chemistry, 91巻, 4号, 2019
  58. Theoretical modeling reveals that regulatory T cells increase T-cell interaction with antigen-presenting cells for stable immune tolerance., International immunology, 31巻, 11号, 2019
  59. Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay., BMC bioinformatics, 20巻, 1号, 2019
  60. Second harmonic generation polarization microscopy as a tool for protein structure analysis., Biophysics and physicobiology, 16巻, 2019
  61. Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells., Stem cell research, 41巻, 2019
  62. Advanced CUBIC tissue clearing for whole-organ cell profiling., Nature protocols, 14巻, 12号, 2019
  63. In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice., Cancer research, 67巻, 3号, 2007
  64. An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation, CHEMICAL COMMUNICATIONS, 56巻, 25号, pp. 3625-3628, 20200328
  65. Multiplexed 129 Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells, Contrast Media & Molecular Imaging, 2020
  66. Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy, ANALYTICAL CHEMISTRY, 92巻, 22号, pp. 14915-14923, 20201117
  67. Quantitation of Cell Membrane Permeability of Cyclic Peptides by Single-Cell Cytoplasm Mass Spectrometry, ANALYTICAL CHEMISTRY, 93巻, 7号, pp. 3370-3377, 20210223
  68. Activation probability of a single naive T cell upon TCR ligation is controlled by T cells interacting with the same antigen-presenting cell, FEBS LETTERS, 595巻, 11号, pp. 1512-1524, 202106
  69. Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment, BIOPHYSICS AND PHYSICOBIOLOGY, 18巻, pp. 145-158, 2021
  70. Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy, OPTICS EXPRESS, 29巻, 15号, pp. 24278-24288, 20210719
  71. Pressure-induced changes on the morphology and gene expression in mammalian cells, BIOLOGY OPEN, 10巻, 7号, 202107
  72. Exploring rare cellular activity in more than one million cells by a transscale scope, SCIENTIFIC REPORTS, 11巻, 1号, 20210816

招待講演、口頭・ポスター発表等

  1. ラマン散乱スペクトルの揺らぎによる細胞状態遷移予測, 渡邉朋信, 日本生化学会北陸支部シンポジウム, 2022年06月04日, 招待, 日本語
  2. 「豊か」になる考え方, 智辯学園奈良カレッジ講演会, 2021年10月30日, 招待, 日本語
  3. 光を当てただけ!?散乱光を用いた生命科学研究ツールの開発, 渡邉朋信, 統合生命科学研究科シンポジウム, 2021年06月21日, 招待, 日本語
  4. 光を当てただけ!?散乱光を用いた生命科学研究ツールの開発, 渡邉朋信, 神戸学院大学セミナー, 2021年07月21日, 招待, 日本語