TOMONOBU WATANABE
Last Updated :2024/10/30
- Affiliations, Positions
- Research Institute for Radiation Biology and Medicine, Professor
- E-mail
- twatanabehiroshima-u.ac.jp
Basic Information
Academic Degrees
- Osaka University
- Osaka University
Educational Activity
Course in Charge
- 2024, Liberal Arts Education Program1, 2Term, Foundation physics for life science
- 2024, Undergraduate Education, Intensive, Practice for medical research
- 2024, Graduate Education (Master's Program) , First Semester, Seminar
- 2024, Graduate Education (Master's Program) , Second Semester, Seminar
- 2024, Graduate Education (Master's Program) , First Semester, Research
- 2024, Graduate Education (Master's Program) , Second Semester, Research
- 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar
- 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar
- 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research
- 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research
- 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Seminar on Stem Cell Biology
- 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Seminar on Stem Cell Biology
- 2024, Graduate Education (Doctoral Program) , First Semester, Advanced Research on Stem Cell Biology
- 2024, Graduate Education (Doctoral Program) , Second Semester, Advanced Research on Stem Cell Biology
- 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar A
- 2024, Graduate Education (Master's Program) , 3Term, Biomedical Science Seminar B
- 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar C
- 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar D
- 2024, Graduate Education (Doctoral Program) , 3Term, Biomedical Science Seminar E
Research Activities
Academic Papers
- Visualizing Singularity Phenomenon, Biophysics and Physicobiology, 202402
- Yuragi biomarker concept for evaluating human induced pluripotent stem cells using heterogeneity-based Raman finger-printing, BIOPHYSICS AND PHYSICOBIOLOGY, 21, 2024
- Quantitative prediction of rice starch digestibility using Raman spectroscopy and multivariate calibration analysis, FOOD CHEMISTRY, 435, 20240301
- Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance, EMBO JOURNAL, 42(18), 20230918
- Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation, LIFE SCIENCE ALLIANCE, 6(8), 202308
- Application of the Dynamical Network Biomarker Theory to Raman Spectra, BIOMOLECULES, 12(12), 202212
- Recent Advances in Raman Spectral Imaging in Cell Diagnosis and Gene Expression Prediction, GENES, 13(11), 202211
- Mitochondria-associated myosin 19 processively transports mitochondria on actin tracks in living cells, Journal of Biological Chemistry, 20220320
- Lattice-patterned collagen fibers and their dynamics in axolotl skin regeneration, ISCIENCE, 25(7), 20220715
- Valosin-containing protein regulates the stability of fused in sarcoma granules in cells by changing ATP concentrations, FEBS LETTERS, 596(11), 1412-1423, 202206
- Non-label immune cell state prediction using Raman spectroscopy., Scientific reports, 6, 2016
- Single- and multiple-molecule dynamics of the signaling from H-Ras to cRaf-1 visualized on the plasma membrane of living cells., Chemphyschem : a European journal of chemical physics and physical chemistry, 4(7), 2003
- A one-headed class V myosin molecule develops multiple large (approximately 32-nm) steps successively., Proceedings of the National Academy of Sciences of the United States of America, 101(26), 2004
- Single-molecule analysis of chemoattractant-stimulated membrane recruitment of a PH-domain-containing protein., Journal of cell science, 119(Pt 6), 2006
- Overlapping hand-over-hand mechanism of single molecular motility of cytoplasmic dynein., Proceedings of the National Academy of Sciences of the United States of America, 103(15), 2006
- Reduction in nonfluorescence state of quantum dots on an immunofluorescence staining., Biochemical and biophysical research communications, 351(1), 2006
- Input-output relationship in galvanotactic response of Dictyostelium cells., Bio Systems, 88(3), 2007
- Stepwise movements in vesicle transport of HER2 by motor proteins in living cells., Biophysical journal, 92(11), 2007
- Three-dimensional nanometry of vesicle transport in living cells using dual-focus imaging optics., Biochemical and biophysical research communications, 359(1), 2007
- Human myosin Vc is a low duty ratio nonprocessive motor., The Journal of biological chemistry, 283(16), 2008
- In vivo nano-imaging of membrane dynamics in metastatic tumor cells using quantum dots., The Journal of biological chemistry, 285(4), 2010
- Myosin-X induces filopodia by multiple elongation mechanism., The Journal of biological chemistry, 285(25), 2010
- Single molecular observation of self-regulated kinesin motility., Biochemistry, 49(22), 2010
- Identification of three distinct functional sites of insulin-mediated GLUT4 trafficking in adipocytes using quantitative single molecule imaging., Molecular biology of the cell, 21(15), 2010
- Mechanical characterization of one-headed myosin-V using optical tweezers., PloS one, 5(8), 2010
- Antibody-protein A conjugated quantum dots for multiplexed imaging of surface receptors in living cells., Molecular bioSystems, 6(11), 2010
- Real-time nanoscopy by using blinking enhanced quantum dots., Biophysical journal, 99(7), 2010
- Synthesis and Characterization of Anti-HER2 Antibody Conjugated CdSe/CdZnS Quantum Dots for Fluorescence Imaging of Breast Cancer Cells., Sensors (Basel, Switzerland), 9(11), 2009
- Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts., Biochemical and biophysical research communications, 418(4), 2012
- Engineering strain-sensitive yellow fluorescent protein., Chemical communications (Cambridge, England), 48(63), 2012
- Atg9 vesicles are an important membrane source during early steps of autophagosome formation., The Journal of cell biology, 198(2), 2012
- Multilayered, core/shell nanoprobes based on magnetic ferric oxide particles and quantum dots for multimodality imaging of breast cancer tumors., Biomaterials, 33(33), 2012
- Distinct modulated pupil function system for real-time imaging of living cells., PloS one, 7(9), 2012
- Improving spinning disk confocal microscopy by preventing pinhole cross-talk for intravital imaging., Proceedings of the National Academy of Sciences of the United States of America, 110(9), 2013
- Rab6a releases LIS1 from a dynein idling complex and activates dynein for retrograde movement., Nature communications, 4, 2013
- Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods., Biophysical journal, 105(3), 2013
- Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure., PloS one, 8(8), 2013
- Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers., Journal of bioscience and bioengineering, 117(6), 2014
- Visualizing cell state transition using Raman spectroscopy., PloS one, 9(1), 2014
- Whole-brain imaging with single-cell resolution using chemical cocktails and computational analysis., Cell, 157(3), 2014
- Nano-scale measurement of biomolecules by optical microscopy and semiconductor nanoparticles., Frontiers in physiology, 5, 2014
- Gene dynamics of core transcription factors for pluripotency in embryonic stem cells., Journal of bioscience and bioengineering, 119(4), 2015
- Expanded palette of Nano-lanterns for real-time multicolor luminescence imaging., Proceedings of the National Academy of Sciences of the United States of America, 112(14), 2015
- Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging., Scientific reports, 5, 2015
- SH3 domain of c-Src governs its dynamics at focal adhesions and the cell membrane., The FEBS journal, 282(20), 2015
- Predictive diagnosis of the risk of breast cancer recurrence after surgery by single-particle quantum dot imaging., Scientific reports, 5, 2015
- Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy., Applied optics, 55(5), 2016
- Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it., Scientific reports, 6, 2016
- Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy: erratum., Applied optics, 55(15), 2016
- Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots., Biomedical optics express, 7(7), 2016
- Activated full-length myosin-X moves processively on filopodia with large steps toward diverse two-dimensional directions., Scientific reports, 7, 2017
- Human myosin VIIa is a very slow processive motor protein on various cellular actin structures., The Journal of biological chemistry, 292(26), 2017
- Design and development of genetically encoded fluorescent sensors to monitor intracellular chemical and physical parameters., Biophysical reviews, 8(2), 2016
- A novel c-Src recruitment pathway from the cytosol to focal adhesions., FEBS letters, 591(13), 2017
- Raman spectroscopy as a tool for ecology and evolution., Journal of the Royal Society, Interface, 14(131), 2017
- In vivo analysis of protein crowding within the nuclear pore complex in interphase and mitosis., Scientific reports, 7(1), 2017
- The use of a genetically encoded molecular crowding sensor in various biological phenomena., Biophysics and physicobiology, 14, 2017
- Myosin X is recruited to nascent focal adhesions at the leading edge and induces multi-cycle filopodial elongation., Scientific reports, 7(1), 2017
- Validation of Common Housekeeping Genes as Reference for qPCR Gene Expression Analysis During iPS Reprogramming Process., Scientific reports, 8(1), 2018
- Cell type discrimination based on image features of molecular component distribution., Scientific reports, 8(1), 2018
- Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation., Scientific reports, 8(1), 2018
- Raman spectral signature reflects transcriptomic features of antibiotic resistance in Escherichia coli., Communications biology, 1, 2018
- Kinesin-binding-triggered conformation switching of microtubules contributes to polarized transport., The Journal of cell biology, 217(12), 2018
- Single-Cell Screening of Tamoxifen Abundance and Effect Using Mass Spectrometry and Raman-Spectroscopy., Analytical chemistry, 91(4), 2019
- Theoretical modeling reveals that regulatory T cells increase T-cell interaction with antigen-presenting cells for stable immune tolerance., International immunology, 31(11), 2019
- Pairwise efficiency: a new mathematical approach to qPCR data analysis increases the precision of the calibration curve assay., BMC bioinformatics, 20(1), 2019
- Second harmonic generation polarization microscopy as a tool for protein structure analysis., Biophysics and physicobiology, 16, 2019
- Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells., Stem cell research, 41, 2019
- Advanced CUBIC tissue clearing for whole-organ cell profiling., Nature protocols, 14(12), 2019
- In vivo real-time tracking of single quantum dots conjugated with monoclonal anti-HER2 antibody in tumors of mice., Cancer research, 67(3), 2007
- An improved fluorescent protein-based expression reporter system that utilizes bioluminescence resonance energy transfer and peptide-assisted complementation, CHEMICAL COMMUNICATIONS, 56(25), 3625-3628, 20200328
- Multiplexed 129 Xe HyperCEST MRI Detection of Genetically Reconstituted Bacterial Protein Nanoparticles in Human Cancer Cells, Contrast Media & Molecular Imaging, 2020
- Following Embryonic Stem Cells, Their Differentiated Progeny, and Cell-State Changes During iPS Reprogramming by Raman Spectroscopy, ANALYTICAL CHEMISTRY, 92(22), 14915-14923, 20201117
- Quantitation of Cell Membrane Permeability of Cyclic Peptides by Single-Cell Cytoplasm Mass Spectrometry, ANALYTICAL CHEMISTRY, 93(7), 3370-3377, 20210223
- Activation probability of a single naive T cell upon TCR ligation is controlled by T cells interacting with the same antigen-presenting cell, FEBS LETTERS, 595(11), 1512-1524, 202106
- Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment, BIOPHYSICS AND PHYSICOBIOLOGY, 18, 145-158, 2021
- Improvement in image quality via the pseudo confocal effect in multidirectional digital scanned laser light-sheet microscopy, OPTICS EXPRESS, 29(15), 24278-24288, 20210719
- Pressure-induced changes on the morphology and gene expression in mammalian cells, BIOLOGY OPEN, 10(7), 202107
- Exploring rare cellular activity in more than one million cells by a transscale scope, SCIENTIFIC REPORTS, 11(1), 20210816