藤田 英明HIDEAKI FUJITA
Last Updated :2024/12/02
- 所属・職名
- 原爆放射線医科学研究所 助教
- メールアドレス
- fujitah
hiroshima-u.ac.jp
基本情報
学位
- 博士（理学） (早稲田大学)
- 修士（理学） (早稲田大学)
教育活動
授業担当
- 2024年, 教養教育, ２ターム, 初修物理学[1医,1歯歯,1歯工,1薬]
- 2024年, 学部専門, 集中, 医学研究実習
- 2024年, 博士課程・博士課程後期, セメスター（前期）, 幹細胞機能学特別演習
- 2024年, 博士課程・博士課程後期, セメスター（後期）, 幹細胞機能学特別演習
- 2024年, 博士課程・博士課程後期, セメスター（後期）, 放射線生物学入門
研究活動
学術論文（★は代表的な論文）
- Yuragi biomarker concept for evaluating human induced pluripotent stem cells using heterogeneity-based Raman finger-printing, BIOPHYSICS AND PHYSICOBIOLOGY, 21巻, 2024
- Bone morphogenetic protein signaling inhibitor improves differentiation and function of 3D muscle construct fabricated using C2C12, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 137巻, 6号, pp. 480-486, 202406
- Single-molecule tracking of Nanog and Oct4 in living mouse embryonic stem cells uncovers a feedback mechanism of pluripotency maintenance, EMBO JOURNAL, 42巻, 18号, 20230918
- Estimation of crossbridge-state during cardiomyocyte beating using second harmonic generation, LIFE SCIENCE ALLIANCE, 6巻, 8号, 202308
- Progress of Endogenous and Exogenous Nanoparticles for Cancer Therapy and Diagnostics, GENES, 14巻, 2号, 202302
- Application of the Dynamical Network Biomarker Theory to Raman Spectra, BIOMOLECULES, 12巻, 12号, 202212
- Recent Advances in Raman Spectral Imaging in Cell Diagnosis and Gene Expression Prediction, GENES, 13巻, 11号, 202211
- Recent advances in animal cell technologies for industrial and medical applications, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 133巻, 6号, pp. 509-514, 202206
- A novel c‐Src recruitment pathway from the cytosol to focal adhesions, Febs Letters, 591巻, 13号, pp. 1940-1946, 2017
- Non-label immune cell state prediction using Raman spectroscopy, Scientific reports, 6巻, 37562号, 2016
- Visualizing the appearance and disappearance of the attractor of differentiation using Raman spectral imaging, Scientific reports, 5巻, 11358号, 2015
- SH 3 domain of c‐Src governs its dynamics at focal adhesions and the cell membrane, The FEBS journal, 282巻, 20号, pp. 4034-4055, 2015
- Effect of cell-extracellular matrix interaction on myogenic characteristics and artificial skeletal muscle tissue, Journal of Bioscience and Bioengineering, 130巻, 1号, pp. 98-105, 2020
- Linking substrate and nucleus via actin cytoskeleton in pluripotency maintenance of mouse embryonic stem cells, Stem cell research, 41巻, pp. 101614, 2019
- Eurotium cristatum fermented okara as a potential food ingredient to combat diabetes, Scientific reports, 9巻, 1号, pp. 1-9, 2019
- Theoretical modeling reveals that regulatory T cells increase T-cell interaction with antigen-presenting cells for stable immune tolerance, International immunology, 31巻, 11号, pp. 743-753, 2019
- Single cell analysis reveals a biophysical aspect of collective cell-state transition in embryonic stem cell differentiation, Scientific reports, 8巻, 1号, pp. 1-13, 2018
- Cell type discrimination based on image features of molecular component distribution, Scientific reports, 8巻, 1号, pp. 1-8, 2018
- Raman spectral signature reflects transcriptomic features of antibiotic resistance in escherichia coli, Communications biology, 1巻, 1号, pp. 1-10, 2018
- Substrate rigidity-dependent positive feedback regulation between YAP and ROCK2, Cell adhesion & migration, 12巻, 2号, pp. 101-108, 2018
- Design and fabrication of devices for investigating cell-sheet stretch, BioChip Journal, 11巻, 3号, pp. 173-179, 2017
- Raman spectroscopy as a tool for ecology and evolution, Journal of The Royal Society Interface, 14巻, 131号, pp. 20170174, 2017
- Protein expression guided chemical profiling of living cells by the simultaneous observation of Raman scattering and anti-Stokes fluorescence emission, Scientific reports, 7巻, pp. 43569, 2017
- Substrate stiffness influences doxorubicin-induced p53 activation via ROCK2 expression, BioMed Research International, 2017巻, 2017
- The use of a genetically encoded molecular crowding sensor in various biological phenomena, Biophysics and Physicobiology, 14巻, pp. 119-125, 2017
- Simultaneous nano-tracking of multiple motor proteins via spectral discrimination of quantum dots, Biomedical optics express, 7巻, 7号, pp. 2475-2493, 2016
- Dependence of fluorescent protein brightness on protein concentration in solution and enhancement of it, Scientific reports, 6巻, pp. 22342, 2016
- Full control of polarization state with a pair of electro-optic modulators for polarization-resolved optical microscopy, Applied optics, 55巻, 5号, pp. 1082-1089, 2016
- Particle simulation of oxidation induced band 3 clustering in human erythrocytes, PLoS Comput Biol, 11巻, 6号, pp. e1004210, 2015
- Fluid driving system for a micropump by differentiating iPS cells into cardiomyocytes on a tent-like structure, Sensors and Actuators B: Chemical, 210巻, pp. 267-272, 2015
- Gene dynamics of core transcription factors for pluripotency in embryonic stem cells, Journal of bioscience and bioengineering, 119巻, 4号, pp. 406-409, 2015
- Comprehensive chemical secretory measurement of single cells trapped in a micro-droplet array with mass spectrometry, RSC advances, 5巻, 22号, pp. 16968-16971, 2015
- Displacement of p130Cas from focal adhesions links actomyosin contraction to cell migration, Journal of cell science, 127巻, 16号, pp. 3440-3450, 2014
- Culturing of mouse and human cells on soft substrates promote the expression of stem cell markers, Journal of bioscience and bioengineering, 117巻, 6号, pp. 749-755, 2014
- Induction of functional tissue-engineered skeletal muscle constructs by defined electrical stimulation, Scientific reports, 4巻, pp. 4781, 2014
- Visualizing cell state transition using Raman spectroscopy, PLoS One, 9巻, 1号, pp. e84478, 2014
- Metabolic flux analysis of genetically engineered Saccharomyces cerevisiae that produces lactate under micro-aerobic conditions, Bioprocess and biosystems engineering, 36巻, 9号, pp. 1261-1265, 2013
- Glycine insertion makes yellow fluorescent protein sensitive to hydrostatic pressure, PLoS One, 8巻, 8号, pp. e73212, 2013
- Four-dimensional spatial nanometry of single particles in living cells using polarized quantum rods, Biophysical journal, 105巻, 3号, pp. 555-564, 2013
- Heart extracellular matrix supports cardiomyocyte differentiation of mouse embryonic stem cells, Journal of bioscience and bioengineering, 115巻, 3号, pp. 320-325, 2013
- Fed-batch system for cultivating genetically engineered yeast that produces lactic acid via the fermentative promoter, Journal of bioscience and bioengineering, 115巻, 2号, pp. 193-195, 2013
- Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators, Journal of bioscience and bioengineering, 115巻, 2号, pp. 115-121, 2013
- Distinct Modulated Pupil Function System for Real-Time Imaging of Living Cells, Plos one, 7巻, 9号, pp. e44028, 2012
- p130Cas-dependent actin remodelling regulates myogenic differentiation, Biochemical Journal, 445巻, 3号, pp. 323-332, 2012
- Chromatin plasticity as a differentiation index during muscle differentiation of C2C12 myoblasts, Biochemical and biophysical research communications, 418巻, 4号, pp. 742-747, 2012
- Engineering strain-sensitive yellow fluorescent protein, Chemical Communications, 48巻, 63号, pp. 7871-7873, 2012
- Designing of a Si-MEMS device with an integrated skeletal muscle cell-based bio-actuator, Biomedical microdevices, 13巻, 1号, pp. 123-129, 2011
- Thin filament-reconstituted skinned muscle fibers for the study of muscle physiology, Journal of Biomedicine and Biotechnology, 2011巻, 2011
- Functional evaluation of artificial skeletal muscle tissue constructs fabricated by a magnetic force-based tissue engineering technique, Tissue Engineering Part A, 17巻, 1-2号, pp. 107-114, 2011
- Rapid decrease in active tension generated by C2C12 myotubes after termination of artificial exercise, Journal of muscle research and cell motility, 31巻, 4号, pp. 279-288, 2010
- Evaluation of serum‐free differentiation conditions for C2C12 myoblast cells assessed as to active tension generation capability, Biotechnology and bioengineering, 107巻, 5号, pp. 894-901, 2010
- Enhancement of C2C12 differentiation by perfluorocarbon-mediated oxygen delivery, Journal of bioscience and bioengineering, 110巻, 3号, pp. 359-362, 2010
- Fabrication of scaffold‐free contractile skeletal muscle tissue using magnetite‐incorporated myogenic C2C12 cells, Journal of tissue engineering and regenerative medicine, 4巻, 6号, pp. 437-443, 2010
- Identification of three distinct functional sites of insulin-mediated GLUT4 trafficking in adipocytes using quantitative single molecule imaging, Molecular biology of the cell, 21巻, 15号, pp. 2721-2731, 2010
- Novel method for measuring active tension generation by C2C12 myotube using UV‐crosslinked collagen film, Biotechnology and bioengineering, 106巻, 3号, pp. 482-489, 2010
- Oxygen plasma‐treated thermoresponsive polymer surfaces for cell sheet engineering, Biotechnology and bioengineering, 106巻, 2号, pp. 303-310, 2010
- Assembly of skeletal muscle cells on a Si-MEMS device and their generative force measurement, Biomedical microdevices, 12巻, 2号, pp. 247-252, 2010
- Micropatterning of single myotubes on a thermoresponsive culture surface using elastic stencil membranes for single-cell analysis, Journal of bioscience and bioengineering, 109巻, 2号, pp. 174-178, 2010
- Magnetic force-based tissue engineering of skeletal muscle, Journal of Biotechnology, 150巻, pp. 441, 2010
- Preparation of artificial skeletal muscle tissues by a magnetic force-based tissue engineering technique, Journal of bioscience and bioengineering, 108巻, 6号, pp. 538-543, 2009
- Novel method for fabrication of skeletal muscle construct from the C2C12 myoblast cell line using serum‐free medium AIM‐V, Biotechnology and bioengineering, 103巻, 5号, pp. 1034-1041, 2009
- Alignment of skeletal muscle myoblasts and myotubes using linear micropatterned surfaces ground with abrasives, Biotechnology and bioengineering, 103巻, 3号, pp. 631-638, 2009
- Application of a cell sheet–polymer film complex with temperature sensitivity for increased mechanical strength and cell alignment capability, Biotechnology and bioengineering, 103巻, 2号, pp. 370-377, 2009
- Contractile C2C12 myotube model for studying exercise-inducible responses in skeletal muscle, American Journal of Physiology-Endocrinology and Metabolism, 295巻, 5号, pp. E1191-E1204, 2008
- Accelerated de novo sarcomere assembly by electric pulse stimulation in C2C12 myotubes, Experimental cell research, 313巻, 9号, pp. 1853-1865, 2007
- A new muscle contractile system composed of a thick filament lattice and a single actin filament, Biophysical journal, 89巻, 1号, pp. 321-328, 2005
- Auto-oscillations of skinned myocardium correlating with heartbeat, Journal of Muscle Research & Cell Motility, 26巻, 2-3号, pp. 93-101, 2005
- Titin isoform-dependent effect of calcium on passive myocardial tension, American Journal of Physiology-Heart and Circulatory Physiology, 287巻, 6号, pp. H2528-H2534, 2004
- The effect of tropomyosin on force and elementary steps of the cross‐bridge cycle in reconstituted bovine myocardium, The Journal of Physiology, 556巻, 2号, pp. 637-649, 2004
- Calcium-dependent molecular spring elements in the giant protein titin, Proceedings of the National Academy of Sciences, 100巻, 23号, pp. 13716-13721, 2003
- The length of cooperative units on the thin filament in rabbit psoas muscle fibres, Experimental physiology, 87巻, 6号, pp. 691-697, 2002
- Myosin light chain 2 modulates MgADP‐induced contraction in rabbit skeletal and bovine cardiac skinned muscle, The Journal of physiology, 542巻, 1号, pp. 221-229, 2002
- Temperature effect on isometric tension is mediated by regulatory proteins tropomyosin and troponin in bovine myocardium, The Journal of Physiology, 539巻, 1号, pp. 267-276, 2002
- Elementary steps of the cross-bridge cycle in bovine myocardium with and without regulatory proteins, Biophysical Journal, 82巻, 2号, pp. 915-928, 2002
- Tropomyosin modulates pH dependence of isometric tension, Biophysical journal, 77巻, 3号, pp. 1540-1546, 1999
- Regulatory roles of MgADP and calcium in tension development of skinned cardiac muscle, Journal of Muscle Research & Cell Motility, 19巻, 8号, pp. 909-921, 1998
- Spontaneous oscillatory contraction without regulatory proteins in actin filament-reconstituted fibers, Biophysical journal, 75巻, 3号, pp. 1439-1445, 1998
- Spontaneous tension oscillation in skinned bovine cardiac muscle, Pflügers Archiv, 433巻, 1-2号, pp. 1-8, 1996
- Structural and functional reconstitution of thin filaments in the contractile apparatus of cardiac muscle, Biophysical journal, 71巻, 5号, pp. 2307-2318, 1996
- Activation probability of a single naive T cell upon TCR ligation is controlled by T cells interacting with the same antigen-presenting cell, FEBS LETTERS, 595巻, 11号, pp. 1512-1524, 202106
- Effect of hydrogen peroxide concentration on the maintenance and differentiation of cultured skeletal muscle cells, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 131巻, 5号, pp. 572-578, 202105
- Glycine insertion modulates the fluorescence properties of Aequorea victoria green fluorescent protein and its variants in their ambient environment, BIOPHYSICS AND PHYSICOBIOLOGY, 18巻, pp. 145-158, 2021
- Pressure-induced changes on the morphology and gene expression in mammalian cells, BIOLOGY OPEN, 10巻, 7号, 202107
- Microarray profiling of gene expression in C2C12 myotubes trained by electric pulse stimulation, JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 132巻, 4号, pp. 417-422, 202110
著書等出版物
- 2022年02月28日, ラマン分光スペクトルデータ解析事例集, ラマン散乱スペクトルと機械学習手法による細胞種類、状態の識別, 単行本（学術書）, 共著, 日, 田村 耕平、塚田 真也、片山 詔久、岸田 英夫、森田 成昭、毛利 真一郎、宮下 喜好、藤井 康裕、福岡 隆夫、正井 博和、福永 悠、中嶋 悟、岡田 哲男、篠崎 浩子、渡邉 朋信、鷹林 将、藤田 英明、藤井 直美、前田 寧、吉岡 信明、下赤 卓史、井上 元基、中圓尾 綾、坂根 稔康、橋本 知美、須納瀬 正範、來海 博央、海野 雅司、林 英樹、永川 栄泰、二村 道也、鍋田 真弓、福田 徳生、竹井 弘之、松本 拓也、源川 拓磨、西 野孝、中林 孝和、松尾 尚子、柴田 大輝、光山 昌宏、高橋 大智、鈴木 鋭二、梶本 真司、清野 智志、山越 博幸、岡村 槙二、、江越 脩祐、西山 博通、袖岡 幹子、犬飼 潤治、藤田 克昌、伊藤 隆、重藤 真介、池田 勝佳、赤木 祐香、須田 潤、木田 泰之、杉江 隆一、大嶋 佑介、梁剣波、南川 丈夫、市川 公善、奈良 雅之、寺地 徳之、丸山 雄介、小泉 聡 、服部 淳彦, ISBN:978-4-86104-872-2, 第４章第３節
招待講演、口頭・ポスター発表等
- Effects of radiation on human iPSC derived cardiomyocyte myosin function evaluated by SHG microscopy, 藤田英明、渡邉朋信, 2024年02月10日, 通常, 英語, フィラデルフィア, その他
- 人工多能性幹細胞とラマン顕微鏡を用いた放射線感受性の個人差推定法, 藤田英明、佐々木健介、渡邉朋信, 第61回生物物理学会年会, 2023年11月14日, 通常, 日本語, 日本生物物理学会, 名古屋, その他
- iPS細胞と光計測技術で晩発的な放射線被ばく障害を予測する, 藤田英明、渡邉朋信, 第6回放射線災害・医科学研究拠点カンファランス, 2022年06月04日, 通常, 日本語, 我々は、幹細胞に対する低線量被ばくの晩発性細胞機能障害を予測するための顕微鏡技術、特に非染色観察系の開発をおこなっている。今回、第二次高調波顕微鏡とラマン散乱顕微鏡について紹介する。
第二次高調波は、光が非中心対象分子にあたったとき、入射光の1/2の波長の光が発生する現象で、生体物質では、コラーゲンや微小管、ミオシンフィラメントが第二次高調波を強く発生させることが知られている。ミオシンフィラメントの場合、第二次高調波強度の偏光依存性は、結合しているミオシンヘッドの割合を示すため、収縮中の筋肉内でどれだけのミオシン分子が働いているかが推察できる。今回、紫外線照射によって放射線暴露を模倣し、ヒトiPS細胞に紫外線照射した後、心筋に分化誘導をおこなった。得られた心筋細胞は自発的拍動が観察されたが、第二次高調波顕微鏡を用いた観察から、紫外線照射を受けたiPS細胞から分化した心筋細胞内では、力発生に寄与しない強結合クロスブリッジの割合が増加していることが判明した（図１）。
ラマン散乱は、分子に入射した光が散乱するとき、一部が分子の固有振動数に由来するエネルギーを分子と受け渡すことにより、入射光とは波長が異なる光が散乱される現象である。波長のずれは物質固有なため、物質からのラマン散乱スペクトルを調べれば、物質を同定することができる。細胞内には様々な物質が含まれているため、細胞からのラマンスペクトルは複雑なものになるが、細胞内の物質構成は細胞状態に依存するため、細胞のラマン散乱スペクトルは細胞状態を反映する（図２）。我々は、低線量放射線暴露による細胞状態の変化を、細胞からのラマン散乱スペクトルによってとらえようとしている。
上記２手法は、どちらも染色や固定などの前処理を必要としない手法である。今後はこれらの手法を用いて、幹細胞に対する低線量放射線暴露の晩発性細胞機能障害予測を行っていく予定である。
- Raman spectral analysis of induced pluripotent stem cell during spontaneous differentiation, 第60回生物物理学会年会, 2022年09月29日, 通常, 日本語, 日本生物物理学会, 函館
- Detection of the onset of Radiation induced cell damage using human iPSC and Raman spectroscopy, 放射線災害・医科学研究拠点国際シンポジウム2022, 2022年02月07日, 通常, 英語
- Evaluation of the effects of hydrogen peroxide on C2C12 derived myotubes, 放射線災害・医科学研究拠点国際シンポジウム2021, 2021年02月08日, 通常, 英語, オンライン
- Fluctuation of Raman scattering can be an initial marker for iPSC differentiation, Hideaki Fujita, Kensuke Sasaki, Kazuhiro Sudo, Yumiko Koga, Yukio Nakamura, Kuniya Abe, Yasuhiko Yoshida, Takayuki Haruki, Keiichi Koizumi, Tomonobu M. Watanabe, Biophysical Society Annual Meeting USA, 2023年02月18日, 通常, 英語
社会活動
学術会議等の主催
- 第１回シンギュラリティー生物学×放射線医科学研究会, 2310年06月, 2310年06月
- 第4回骨格筋スマート社会実現コンソーシアム講演会, オーガナイザー, 2021年09月, 2021年09月
- 2021 国際大学連携動物細胞研究会, 大会実行委員長, 2021年09月, 2021年09月
- 第42回動物細胞工学会2021年度大会, 大会実行委員, 2021年07月, 2021年07月
- 2021シンギュラリティ生物学Web交流会, オーガナイザー, 2021年04月, 2022年03月
- 化学工学会第52会春季大会バイオ部会シンポジウム, オーガナイザー, 2021年09月, 2021年09月
- 2022シンギュラリティ生物学Web交流会, オーガナイザー, 2022年04月, 2023年03月
- 第5回骨格筋スマート社会実現コンソーシアム講演会, オーガナイザー, 2022年11月, 2022年11月
