大前 英司Eiji Oomae

Last Updated :2022/08/02

所属・職名
大学院統合生命科学研究科 助教
メールアドレス
ohmaehiroshima-u.ac.jp

基本情報

学位

  • 博士(理学) (広島大学)
  • 修士(農学) (名古屋大学)

研究分野

  • 生物学 / 生物科学 / 生物物理学

研究キーワード

  • 酵素の機能
  • 酵素の構造
  • 高圧力
  • 深海酵素

教育活動

授業担当

  1. 2022年, 学部専門, 1ターム, 化学概説A
  2. 2022年, 学部専門, 4ターム, 先端化学
  3. 2022年, 学部専門, セメスター(前期), 物理化学演習
  4. 2022年, 学部専門, 3ターム, バイオインフォマティクス
  5. 2022年, 学部専門, セメスター(前期), 化学実験I
  6. 2022年, 学部専門, セメスター(後期), 化学実験II
  7. 2022年, 修士課程・博士課程前期, 2ターム, プロテオミクス
  8. 2022年, 修士課程・博士課程前期, 2ターム, 生命理学特論C
  9. 2022年, 修士課程・博士課程前期, 4ターム, 生命理学特論D

研究活動

学術論文(★は代表的な論文)

  1. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp. 1115-1121, 2008
  2. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp. 67-71, 200909
  3. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp. 846-855, 201004
  4. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp. 591-599, 201004
  5. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp. 165-175, 201103
  6. Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium \textit{Shewanella violacea} Strain DSS12, Chemistry Letters, 33巻, 7号, pp. 798-799, 20040701
  7. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, Nucreic Acids Research, 32巻, 17号, pp. 5291-5302, 20041001
  8. ジヒドロ葉酸還元酵素のループ機能解析, 生物物理, 44巻, 2号, pp. 70-74, 20040401
  9. 高圧下における酵素科学, 月刊海洋, 36巻, 9号, pp. 692-697, 20040901
  10. Effects of mutation and ligand binding on the compressibility of protein, Progress in Biotechnology, 19巻, pp. 63-70, 20020401
  11. Mass spectrometry of hydrogen/deuterium exchange of \textit{Escherichia coli} dihydrofolate reductase: Effects of loop mutations, The Journal of Biochemistry, 135巻, pp. 487-494, 20040401
  12. Effects of point mutations at the flexible loop glycine-67 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 119巻, pp. 703-710, 19960401
  13. Acid and thermal unfolding of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 120巻, pp. 946-953, 19960401
  14. A large compressibility change of protein induced by a single amino acid substitution, Protein Science, 5巻, pp. 542-545, 19960401
  15. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp. 33-41, 19980401
  16. Effects of point mutations at the flexible loop alanine-145 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp. 839-846, 19980401
  17. Acetonitrile-protein interactions; amino acid solubility and preferential solvation, Biochimica et Biophysica Acta, 1387巻, pp. 195-205, 19980401
  18. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, Chemistry Letters, 1999巻, pp. 507-508, 19990401
  19. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, The Journal of Biochemistry, 128巻, pp. 21-27, 20000401
  20. High-pressure NMR reveals active-site motion of folate-bound \textit{Escherichia coli} dihydrofolate reductase, Biochemistry, 39巻, pp. 12789-12795, 20000401
  21. Effects of five-tryptophan mutations on structure, stability and function of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 130巻, pp. 439-447, 20010401
  22. Pressure effects on enzyme functions, J. Biol. Macromol., pp. 23-29, 20070701
  23. Discoveries of deep-sea piezophiles, and their pressure adapted enzymes., Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology, 1巻, pp. 114-121, 20071001
  24. Proteins, under high-pressure environments -Discoveries of deep-sea piezophiles, and their pressure adapted enzymes-., The proceedings of International Symposium on Extremophiles and Their Applications, pp. 148-155, 20071001
  25. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions, J. Biochem. (Tokyo), 137巻, pp. 643-672, 20051001
  26. 1000気圧の環境下でもはたらくタンパク質の不思議 深海好圧性微生物の探索とその耐圧酵素, BIONICS, 2巻, 10号, pp. 36-41, 20051001
  27. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: Implication of hydrophobic interactions, JOURNAL OF BIOCHEMISTRY, 137巻, 5号, pp. 643-652, 200505
  28. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp. 511-519, 201203
  29. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp. 111-114, 20130530
  30. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp. 701-709, 201309
  31. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp. 2782-2794, 201312
  32. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 201503
  33. Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119巻, 41号, pp. 13002-13008, 20151015
  34. High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase, BIOCHEMISTRY, 39巻, 42号, pp. 12789-12795, 2000
  35. Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria., Sub-cellular biochemistry, 72巻, 2015
  36. Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution., Extremophiles : life under extreme conditions, 20巻, 2号, 2016
  37. Similar structural stabilities of 3-isopropylmalate dehydrogenases from the obligatory piezophilic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1., Biochimica et biophysica acta, 1866巻, 5-6号
  38. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1., Extremophiles : life under extreme conditions, 21巻, 3号, 2017
  39. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 1号, pp. 33-41, 1998
  40. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 1号, pp. 33-41, 1998
  41. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 1号, pp. 33-41, 1998
  42. Effects of point mutations at the flexible loop alanine-145 of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 5号, pp. 839-846, 1998
  43. Effects of point mutations at the flexible loop alanine-145 of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 5号, pp. 839-846, 1998
  44. Effects of point mutations at the flexible loop alanine-145 of Escherichia coli dihydrofolate reductase on its stability and function, JOURNAL OF BIOCHEMISTRY, 123巻, 5号, pp. 839-846, 1998
  45. Acetonitrile-protein interactions: amino acid solubility and preferential solvation, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1387巻, 1-2号, pp. 195-205, 1998
  46. Acetonitrile-protein interactions: amino acid solubility and preferential solvation, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1387巻, 1-2号, pp. 195-205, 1998
  47. Acetonitrile-protein interactions: amino acid solubility and preferential solvation, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY, 1387巻, 1-2号, pp. 195-205, 1998
  48. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, CHEMISTRY LETTERS, 6号, pp. 507-508, 1999
  49. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, CHEMISTRY LETTERS, 6号, pp. 507-508, 1999
  50. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, CHEMISTRY LETTERS, 6号, pp. 507-508, 1999
  51. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 128巻, 1号, pp. 21-27, 2000
  52. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 128巻, 1号, pp. 21-27, 2000
  53. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 128巻, 1号, pp. 21-27, 2000
  54. Effects of five-tryptophan mutations on structure, stability and function of Escherichia coli dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 130巻, 3号, pp. 439-447, 2001
  55. Effects of five-tryptophan mutations on structure, stability and function of Escherichia coli dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 130巻, 3号, pp. 439-447, 2001
  56. Effects of five-tryptophan mutations on structure, stability and function of Escherichia coli dihydrofolate reductase, JOURNAL OF BIOCHEMISTRY, 130巻, 3号, pp. 439-447, 2001
  57. Mass spectrometry of hydrogen/deuterium exchange of Escherichia coli dihydrofolate reductase: Effects of loop mutations, JOURNAL OF BIOCHEMISTRY, 135巻, 4号, pp. 487-494, 2004
  58. Mass spectrometry of hydrogen/deuterium exchange of Escherichia coli dihydrofolate reductase: Effects of loop mutations, JOURNAL OF BIOCHEMISTRY, 135巻, 4号, pp. 487-494, 2004
  59. Mass spectrometry of hydrogen/deuterium exchange of Escherichia coli dihydrofolate reductase: Effects of loop mutations, JOURNAL OF BIOCHEMISTRY, 135巻, 4号, pp. 487-494, 2004
  60. Pressure-dependent activity of dihydrofolate reductase from a deep-sea bacterium Shewanella violacea strain DSS12, CHEMISTRY LETTERS, 33巻, 7号, pp. 798-799, 2004
  61. Pressure-dependent activity of dihydrofolate reductase from a deep-sea bacterium Shewanella violacea strain DSS12, CHEMISTRY LETTERS, 33巻, 7号, pp. 798-799, 2004
  62. Pressure-dependent activity of dihydrofolate reductase from a deep-sea bacterium Shewanella violacea strain DSS12, CHEMISTRY LETTERS, 33巻, 7号, pp. 798-799, 2004
  63. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, NUCLEIC ACIDS RESEARCH, 32巻, 17号, pp. 5291-5302, 2004
  64. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, NUCLEIC ACIDS RESEARCH, 32巻, 17号, pp. 5291-5302, 2004
  65. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, NUCLEIC ACIDS RESEARCH, 32巻, 17号, pp. 5291-5302, 2004
  66. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: Implication of hydrophobic interactions, JOURNAL OF BIOCHEMISTRY, 137巻, 5号, pp. 643-652, 2005
  67. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: Implication of hydrophobic interactions, JOURNAL OF BIOCHEMISTRY, 137巻, 5号, pp. 643-652, 2005
  68. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: Implication of hydrophobic interactions, JOURNAL OF BIOCHEMISTRY, 137巻, 5号, pp. 643-652, 2005
  69. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp. 1115-1121, 2008
  70. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp. 1115-1121, 2008
  71. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp. 1115-1121, 2008
  72. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp. 67-71, 2009
  73. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp. 67-71, 2009
  74. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp. 67-71, 2009
  75. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp. 846-855, 2010
  76. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp. 846-855, 2010
  77. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp. 846-855, 2010
  78. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp. 591-599, 2010
  79. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp. 591-599, 2010
  80. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp. 591-599, 2010
  81. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp. 165-175, 2011
  82. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp. 165-175, 2011
  83. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp. 165-175, 2011
  84. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp. 511-519, 2012
  85. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp. 511-519, 2012
  86. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp. 511-519, 2012
  87. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp. 111-114, 2013
  88. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp. 111-114, 2013
  89. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp. 111-114, 2013
  90. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp. 701-709, 2013
  91. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp. 701-709, 2013
  92. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp. 701-709, 2013
  93. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp. 2782-2794, 2013
  94. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp. 2782-2794, 2013
  95. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp. 2782-2794, 2013
  96. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 2015
  97. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 2015
  98. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 2015