大前 英司Eiji Oomae

Last Updated :2024/07/03

所属・職名
大学院統合生命科学研究科　助教
メールアドレス
ohmaehiroshima-u.ac.jp

基本情報

学位

  • 博士（理学）　(広島大学)
  • 修士（農学）　(名古屋大学)

研究分野

  • 生物学 / 生物科学 / 生物物理学

研究キーワード

  • 酵素の機能
  • 酵素の構造
  • 高圧力
  • 深海酵素

教育活動

授業担当

  1. 2024年, 学部専門, セメスター（前期）, 物理化学演習
  2. 2024年, 学部専門, ３ターム, バイオインフォマティクス
  3. 2024年, 学部専門, セメスター（前期）, 化学実験I
  4. 2024年, 学部専門, セメスター（後期）, 化学実験II
  5. 2024年, 修士課程・博士課程前期, １ターム, 分子生物物理学
  6. 2024年, 修士課程・博士課程前期, ２ターム, プロテオミクス
  7. 2024年, 修士課程・博士課程前期, ２ターム, 生命理学特論Ｃ
  8. 2024年, 修士課程・博士課程前期, ４ターム, 生命理学特論Ｄ

研究活動

学術論文（★は代表的な論文）

  1. Effects of pressure on enzyme function of Escherichia coli dihydrofolate reductase, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1784巻, 7-8号, pp. 1115-1121, 2008
    DOIを用いた論文検索結果へのリンク
  2. Pressure dependence of the apparent specific volume of bovine serum albumin: Insight into the difference between isothermal and adiabatic compressibilities, BIOPHYSICAL CHEMISTRY, 144巻, 1-2号, pp. 67-71, 200909
    DOIを用いた論文検索結果へのリンク
  3. Coupling effects of distal loops on structural stability and enzymatic activity of Escherichia coli dihydrofolate reductase revealed by deletion mutants, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1804巻, 4号, pp. 846-855, 201004
    DOIを用いた論文検索結果へのリンク
  4. Cloning and characterization of dihydrofolate reductases from deep-sea bacteria, JOURNAL OF BIOCHEMISTRY, 147巻, 4号, pp. 591-599, 201004
    DOIを用いた論文検索結果へのリンク
  5. Comparative study on dihydrofolate reductases from Shewanella species living in deep-sea and ambient atmospheric-pressure environments, EXTREMOPHILES, 15巻, 2号, pp. 165-175, 201103
    DOIを用いた論文検索結果へのリンク
  6. Pressure-dependent Activity of Dihydrofolate Reductase from a Deep-sea Bacterium \textit{Shewanella violacea} Strain DSS12, Chemistry Letters, 33巻, 7号, pp. 798-799, 20040701
  7. Mutational analysis of the damage-recognition and catalytic mechanism of human SMUG1 DNA glycosylase, Nucreic Acids Research, 32巻, 17号, pp. 5291-5302, 20041001
  8. ジヒドロ葉酸還元酵素のループ機能解析, 生物物理, 44巻, 2号, pp. 70-74, 20040401
  9. 高圧下における酵素科学, 月刊海洋, 36巻, 9号, pp. 692-697, 20040901
  10. Effects of mutation and ligand binding on the compressibility of protein, Progress in Biotechnology, 19巻, pp. 63-70, 20020401
  11. Mass spectrometry of hydrogen/deuterium exchange of \textit{Escherichia coli} dihydrofolate reductase: Effects of loop mutations, The Journal of Biochemistry, 135巻, pp. 487-494, 20040401
  12. Effects of point mutations at the flexible loop glycine-67 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 119巻, pp. 703-710, 19960401
  13. Acid and thermal unfolding of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 120巻, pp. 946-953, 19960401
  14. A large compressibility change of protein induced by a single amino acid substitution, Protein Science, 5巻, pp. 542-545, 19960401
  15. Nonadditive effects of double mutations at the flexible loops, glycine-67 and glycine-121, of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp. 33-41, 19980401
  16. Effects of point mutations at the flexible loop alanine-145 of \textit{Escherichia coli} dihydrofolate reductase on its stability and function, The Journal of Biochemistry, 123巻, pp. 839-846, 19980401
  17. Acetonitrile-protein interactions; amino acid solubility and preferential solvation, Biochimica et Biophysica Acta, 1387巻, pp. 195-205, 19980401
  18. Large flexibility of dihydrofolate reductase as revealed by temperature effects on the volume and compressibility, Chemistry Letters, 1999巻, pp. 507-508, 19990401
  19. Single amino acid substitutions in flexible loops can induce large compressibility changes in dihydrofolate reductase, The Journal of Biochemistry, 128巻, pp. 21-27, 20000401
  20. Effects of five-tryptophan mutations on structure, stability and function of \textit{Escherichia coli} dihydrofolate reductase, The Journal of Biochemistry, 130巻, pp. 439-447, 20010401
  21. Pressure effects on enzyme functions, J. Biol. Macromol., pp. 23-29, 20070701
  22. Discoveries of deep-sea piezophiles, and their pressure adapted enzymes., Proceedings of the 4th International Conference on High Pressure Bioscience and Biotechnology, 1巻, pp. 114-121, 20071001
  23. Proteins, under high-pressure environments -Discoveries of deep-sea piezophiles, and their pressure adapted enzymes-., The proceedings of International Symposium on Extremophiles and Their Applications, pp. 148-155, 20071001
  24. Effects of mutation at methionine-42 of Escherichia coli dihydrofolate reductase on stability and function: implication of hydrophobic interactions, J. Biochem. (Tokyo), 137巻, pp. 643-672, 20051001
  25. １０００気圧の環境下でもはたらくタンパク質の不思議　深海好圧性微生物の探索とその耐圧酵素, BIONICS, 2巻, 10号, pp. 36-41, 20051001
  26. Pressure dependence of activity and stability of dihydrofolate reductases of the deep-sea bacterium Moritella profunda and Escherichia coli, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1824巻, 3号, pp. 511-519, 201203
    DOIを用いた論文検索結果へのリンク
  27. Vacuum-ultraviolet circular dichroism of Escherichia coli dihydrofolate reductase: Insight into the contribution of tryptophan residues, CHEMICAL PHYSICS LETTERS, 572巻, pp. 111-114, 20130530
    DOIを用いた論文検索結果へのリンク
  28. Thermodynamic and functional characteristics of deep-sea enzymes revealed by pressure effects, EXTREMOPHILES, 17巻, 5号, pp. 701-709, 201309
    DOIを用いた論文検索結果へのリンク
  29. Solvent environments significantly affect the enzymatic function of Escherichia coli dihydrofolate reductase: Comparison of wild-type protein and active-site mutant D27E, BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 1834巻, 12号, pp. 2782-2794, 201312
    DOIを用いた論文検索結果へのリンク
  30. Effects of salt on the structure, stability, and function of a halophilic dihydrofolate reductase from a hyperhalophilic archaeon, Haloarcula japonica strain TR-1, EXTREMOPHILES, 19巻, 2号, pp. 479-493, 201503
    DOIを用いた論文検索結果へのリンク
  31. Vacuum-Ultraviolet Circular Dichroism Spectra of Escherichia coli Dihydrofolate Reductase and Its Mutants: Contributions of Phenylalanine and Tyrosine Side Chains and Exciton Coupling of Two Tryptophan Side Chains, JOURNAL OF PHYSICAL CHEMISTRY B, 119巻, 41号, pp. 13002-13008, 20151015
    DOIを用いた論文検索結果へのリンク
  32. High pressure NMR reveals active-site hinge motion of folate-bound Escherichia coli dihydrofolate reductase, BIOCHEMISTRY, 39巻, 42号, pp. 12789-12795, 2000
  33. Environmental Adaptation of Dihydrofolate Reductase from Deep-Sea Bacteria., Sub-cellular biochemistry, 72巻, 2015
  34. Pressure adaptation of 3-isopropylmalate dehydrogenase from an extremely piezophilic bacterium is attributed to a single amino acid substitution., Extremophiles : life under extreme conditions, 20巻, 2号, 2016
  35. Similar structural stabilities of 3-isopropylmalate dehydrogenases from the obligatory piezophilic bacterium Shewanella benthica strain DB21MT-2 and its atmospheric congener S. oneidensis strain MR-1., Biochimica et biophysica acta, 1866巻, 5-6号
  36. Halophilic mechanism of the enzymatic function of a moderately halophilic dihydrofolate reductase from Haloarcula japonica strain TR-1., Extremophiles : life under extreme conditions, 21巻, 3号, 2017

著書等出版物

  1. 2019年, 単行本（学術書）, 共著
  2. 2013年, タンパク質の構造安定性と機能における水和の役割 : 深海微生物由来酵素の研究からわかること, 高圧力の科学と技術 / 日本高圧力学会 [編], 大前 英司
  3. Chapter 21. Environmental adaptation of dihydrofolate reductase from deep-sea bacteria., “High Pressure Bioscience” (Eds. Akasaka, K. and Matsuki, H.)/Springer, 大前, 英司;月向, 邦彦;加藤, 千明;Ohmae, Eiji;Gekko, Kunihiko;KATO, CHIAKI